| the phenol oxidases of the ascomycete podospora anserina. xii. affinity of laccases ii and iii to substrates with different substitution patterns. | for the low molecular weight laccases ii and iii of podospora anserina the kinetic parameters michaelis constant (km) and maximum reaction velocity (v) were determined polarographically under ph optimum conditions for representative substrates of different substitution patterns. laccase ii showed two peaks in its ph optimum curve, each with a different substrate specificity, indicating structural differences to laccase iii which exhibits only one broad peak. under optimum conditions the affiniti ... | 1977 | 14600 |
| [electron microscopy of mitochondrial dna in podospora anserina and the presence of a multimeric range of circular dna molecules from senescent cultures]. | mitochondrial dna from young cultures of race s of podospora anserina was isolated. its density in cesium chloride density equilibrium gradients was 1.694 g/cc. examination by the electron microscope revealed that ca 1% of this dna consisted of circles, 31 micrometer in contour length; the remaining dna was composed of linear molecules ranging in length from 2 to 33 micrometer. in dna of similar density obtained from senescent cultures of the same race s, about 11% of the molecules consisted of ... | 1978 | 100254 |
| the phenoloxidases of the ascomycete podospora anserina. structural differences between laccases of high and low molecular weight. | in order to investigate the extent of the relationship between the three copper-containing glycoproteins, laccases i, ii and iii (mr70000, 80000 and 390000 respectively) of podospora anserina, the following experiments were carried out on laccases ii and iii: (a) determination of amino acid composition; (b) determination of n-terminal and c-terminal amino acid; (c) determination of sugar composition; (d) dissociation studies on native and denatured laccases and also after removal of copper from ... | 1978 | 101375 |
| dominant enhancer effect of the meiotic mei4 mutant on recombination frequencies restricted to linkage group vi in podospora anserina. | a mutant which increases second division segregation (sds) frequency of locus 110 (linkage group vi) was isolated. it was called mei4 because of its meiotic deficiency. the present paper deals with its effect on meiotic recombination when heterozygous. mei4 then only acts on linkage group vi. the sds frequencies were increased for all markers used, except locus 5 located very close to the centromere. this quasi general enhancement results exclusively in an enlargement of map distance on linkage ... | 1979 | 289899 |
| evidence for non-random spatial distribution of meiotic exchanges in podospora anserina: comparison between linkage groups 1 and 6. | in podospora anserina, positive and very efficient chiasma interference is observed. however, its modalities are different for the two linkage groups 1 (lg1) and 6 (lg6) studied here. in the right arm of lg1, two zones exist in which always occurs only one crossing-over. they are formed independently each other. moreover, the genetic map consists of clusters of genes located near the centromere and at the limit between the two interference zones. it is postulated that this structure of the map r ... | 1979 | 295403 |
| occurrence of a major protein associated with fruiting body development in neurospora and related ascomycetes. | electrophoretic and immunological analysis of fruiting body (perithecial) extracts demonstrates the occurrence of a major phase-specific perithecial protein in all neurospora species and in the closely related gelasinospora cerealis and sordariafimicola. the perithecial proteins from these different species fall into a number of groups with different electrophoretic mobilities. they appear to be immunologically closely related but not identical to one another even within the same genus, with onl ... | 1977 | 410027 |
| isolation and partial characterization of the multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in the fungus podospora anserina. | three dna-dependent rna polymerases have been isolated and partially purified from the mycelium of the fungus podospora anserina. separated by deae-sephadex chromatography, they have been designated rna polymerases i, ii, and iii according to their order of elution. their catalytic properties and alpha-amanitin sensitivity are in agreement with those of the homologous enzymes found in other eukaryotic organisms. the three enzymes exhibit rather sharp monophasic ammonium sulfate dependence with o ... | 1979 | 574141 |
| ribosomal proteins in the fungus podospora anserina: evidence for an electrophoretically altered 60s protein in a cycloheximide resistant mutant. | proteins of cytoplasmic ribosomes of the podospora anserina were analyzed by two dimensional gel electrophoresis. the numbers of proteins were estimated to be 28 in the small subunit and 41 in the large subunit. the l21 protein of the large subunit was found to migrate differently in a cycloheximide resistant mutant. | 1977 | 600264 |
| quantitative variation of a 60s ribosomal protein during growth of the fungus podospora anserina. | during the growth phase, in the fungus podospora anserina, a variation is observed in the composition of the ribosomal proteins. a protein of the 60s subunit which is absent in the ribosomes from 2 days old cultures becomes gradully more abundant as the culture time is prolonged. | 1977 | 603789 |
| genetic and biochemical analysis of cycloheximide resistance in the fungus podospora anserina. | genetic analysis of cycloheximide-resistant mutants has shown that at least three genes control the resistance to cycloheximide in podospora anserina and that the antibiotic resistance is recessive to sensitivity. in vitro and in vivo studies of protein synthesis indicated that for two mutants cycloheximide resistance is associated with the ribosomes. for one of these mutants, the elongation step in protein biosynthesis is insensitive to cycloheximide over a wide range of concentration. in this ... | 1978 | 678295 |
| cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete podospora anserina. | a double mutant strain combining two ribosomal mutations conferring resistance to cycloheximide exhibits a cold-sensitive phenotype. at low temperature the biosynthesis of the 60s subunit is impaired. genetic analysis of cold-resistant revertants have shown that this double mutant strain can be used efficiently to isolate new ribosomal mutations. | 1978 | 739974 |
| prevention of senescence in the ascomycete podospora anserina by the antibiotic tiamulin. | | 1977 | 834297 |
| mitochondrial genes in podospora anserina: recombination and linkage. | a fifth cytoplasmic mutation (capr 1) obtained in podospora anserina is described. in addition to chloramphenicol resistance it confers a strong deficiency in cytochrome aa3 and impairs the germination of ascospores. genetic analysis shows: 1) strict maternal inheritance of (capr 1) allele; 2) selection against the (capr 1) allele as well in sexual crosses as during vegetative growth; 3) complete reversion of this selection by even low concentration of cap. on the basis of their cytoplasmic inhe ... | 1977 | 887068 |
| genetic evidence of ribosomal antisuppressors in podospora anserina. | antisuppressors were screened for with the help of informational suppressors in podospores anserina. four mutations in the as1 locus and two in the as2 locus were isolated, using allele non specific suppressors supposed to be ribosomal ambiguity mutations. four mutations in the as3 locus and 45 in the as4 locus were obtained, using a nonsense (t-rna like) suppressor. all antisuppressors are partially dominant. most mutations in the as4 locus are lethal. the four mutants at the as3 locus and 6 ou ... | 1976 | 967160 |
| uv and gamma-ray sensitivity of meiosis-deficient mutants in podospora anserina. | two mutants, mei1 and mei2, were isolated by screening for deficiencies occurring in the meiotic process. the sensitivity of mei1 and mei2 mutant strains to uv irradiation showed a significant increase as compared with that of the wild-type stock, whereas the sensitivity to gamma-rays remained unchanged. the double-mutant strains were no more sensitive than each single mutant. the data indicate that both mei1 and mei2 loci are probably involved in the same pathway of excision-repair of uv-induce ... | 1976 | 1012300 |
| cytoplasmic mutations isolated from protoplasts of podospora anserina. | | 1975 | 1183805 |
| the molecular mechanism of protoplasmic incompatibility and its relationship to the formation of protoperithecia in podospora anserina. | in podospora anserina, protoplasmic incompatibility due to interactions between non-allelic genes was suppressed by the effect of mutations in two modifier genes, mod-i and mod-2. it is shown that mod-i and mod-2 are involved in the production of three specific proteins, a phenoloxidase and two previously identified proteases (bégueret & bernet 1973 a) which are associated with the phenomenon of protoplasmic disintegration. these enzymes, whose messengers are normallly latent during vegetative g ... | 1976 | 1245840 |
| genes inhibiting senescence in the ascomycete podospora anserina. | senescence occurs in all wild strains of podospora anserina after continued growth. this syndrome can be inhibited by a synergistic interaction of two linked genes, incoloris and vivax. whereas the wild strain starts to become senescent after 26 d and the mutants incoloris and vivax after 42 and 66 d respectively, the double mutant shows no signs of aging after culture for more than one year. | 1976 | 1264062 |
| regulation of proteolytic enzymes in podospora anserina: selection and properties of self-lysing mutant strains. | previous results showed that cell disintegration in the fungus podospora anserina occured through the action of two proteases, enzymes whose messengers were normally latent during the extension stage of the thallus. we selected three mutant strains in which the constitutive activity of the protease messengers was expressed by an arrest of growth early in development (10 to 30 hours after spore germination) and a reaction of cell disintegration, in the thallus, suppressible with beta-phenyl pyruv ... | 1976 | 1272246 |
| integrative transformation of the ascomycete podospora anserina: identification of the mating-type locus on chromosome vii of electrophoretically separated chromosomes. | protoplasts of wild-type strain s and a long-lived extrachromosomal mutant (al2) of the ascomycete podospora anserina were transformed using a plasmid (pan7-1) which contains the hygromycin b phosphotransferase gene (hph) of escherichia coli under the control of aspergillus nidulans regulatory sequences. after optimizing the transformation procedure, transformation efficiencies of 15-21 transformants/micrograms plasmid dna were obtained. using a second selectable vector (pbt3), which contains th ... | 1991 | 1367277 |
| cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cdna from the plant pathogenic fungus glomerella cingulata. | the glyceraldehyde-3-phosphate dehydrogenase gene (gpda) has been identified from a genomic dna library prepared from the plant pathogenic fungus glomerella cingulata. nucleotide sequence data revealed that this gene codes for a putative 338-amino-acid protein encoded by two exons of 129 and 885 bp, separated by an intron 216 bp long. the 5' leader sequence is also spliced by an intron of 156 bp. a cdna clone was prepared using the polymerase chain reaction, the sequence of which was used to con ... | 1992 | 1452034 |
| the mitochondrial tyrosyl-trna synthetase of podospora anserina is a bifunctional enzyme active in protein synthesis and rna splicing. | the neurospora crassa mitochondrial tyrosyl-trna synthetase (mt tyrrs), which is encoded by the nuclear gene cyt-18, functions not only in aminoacylation but also in the splicing of group i introns. here, we isolated the cognate podospora anserina mt tyrrs gene, designated yts1, by using the n. crassa cyt-18 gene as a hybridization probe. dna sequencing of the p. anserina gene revealed an open reading frame (orf) of 641 amino acids which has significant similarity to other tyrrss. the yts1 orf i ... | 1992 | 1531084 |
| the mating types of podospora anserina: functional analysis and sequence of the fertilization domains. | the two idiomorphic alleles called mat+ and mat-, which control the mating types in podospora anserina, have been cloned. mat+ and mat- encompass 3.8 kb and 4.7 kb respectively, of unrelated dna sequences flanked by common sequences. subcloning allowed the identification and localization in each locus of the gene that controls fertilization, probably by determining the mating type. the mat+ gene, called fpr1, encodes a protein with a potential dna-binding hmg domain. the presence of this motif s ... | 1992 | 1534866 |
| sequence analysis of the gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of podospora anserina: use of homologous regulatory sequences to improve transformation efficiency. | the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of podospora anserina has been isolated from a genomic library by heterologous hybridization with the corresponding gene of curvularia lunata. the coding region consists of 1014 nucleotides and is interrupted by a single intron. the amino-acid sequence encoded by the gpd gene shows a high degree of sequence identity with the corresponding gene products of various fungi. multiple alignments of all fungal gpd sequences so far available result ... | 1992 | 1563046 |
| small subunit ribosomal rna of blastomyces dermatitidis: sequence and phylogenetic analysis. | we determined the small subunit (18s) ribosomal rna sequence of the dimorphic fungus blastomyces dermatitidis. the sequence was compared to that of fourteen other eukaryotic organisms, ten of which were higher fungi, and an evolutionary tree was constructed based on these sequences. b. dermatitidis aligned most closely with the ascomycetes neurospora crassa and podospora anserina, in agreement with previous phylogenetic analysis based on morphological criteria. phase-specific cdna clones derived ... | 1992 | 1564447 |
| identification of a family of bacteriophage t4 genes encoding proteins similar to those present in group i introns of fungi and phage. | the bacteriophage t4 sega gene lies in a genetically unmapped region between the gene beta gt (beta-glucosyltransferase) and uvsx (recombination protein) and encodes a protein of 221 amino acids. we have found that the first 100 amino acids of the sega protein are highly similar to the n termini of four other predicted t4 proteins, also of unknown function. together these five proteins, sega-e (similar to endonucleases of group i introns), contain regions of similarity to the endonuclease i-tev ... | 1992 | 1631169 |
| incipient mitochondrial evolution in yeasts. ii. the complete sequence of the gene coding for cytochrome b in saccharomyces douglasii reveals the presence of both new and conserved introns and discloses major differences in the fixation of mutations in evolution. | we have determined the complete sequence of the mitochondrial gene coding for cytochrome b in saccharomyces douglasii. the gene is 6310 base-pairs long and is interrupted by four introns. the first one (1311 base-pairs) belongs to the group id of secondary structure, contains a fragment open reading frame with a characteristic giy ... yig motif, is absent from saccharomyces cerevisiae and is inserted in the same site in which introns 1 and 2 are inserted in neurospora crassa and podospora anseri ... | 1991 | 1708831 |
| cloning the mating types of the heterothallic fungus podospora anserina: developmental features of haploid transformants carrying both mating types. | dnas that encode the mating-type functions (mat+ and mat-) of the filamentous fungus podospora anserina were cloned with the use of the mating-type a probe from neurospora crassa. cloning the full mat information was ascertained through gene replacement experiments. molecular and functional analyses of haploid transformants carrying both mating types lead to several striking conclusions. mat+ mat- strains are dual maters. however, the resident mat information is dominant to the mat information a ... | 1991 | 1831427 |
| sequence of the nuclear atp synthase subunit 9 gene of podospora anserina: lack of similarity to the mitochondrial genome. | the nuclear gene coding for the mitochondrial subunit 9 of the f0f1-atp synthase complex was isolated from a genomic library of podospora anserina. nucleotide sequencing revealed an open reading frame capable to code for 144 amino acids including an amino-terminal pre-sequence of 63 amino acid residues for mitochondrial import of the pre-proteolipid. the p. anserina proteolipid shows extensive sequence identity with the corresponding gene products of the related filamentous fungi neurospora cras ... | 1991 | 1834355 |
| repa, a repetitive and dispersed dna sequence of the filamentous fungus podospora anserina. | the sequences of homologous dna regions of two wild-type strains of the fungus podospora anserina, revealed in one strain the presence of a 349bp insertion leading to a rflp. this dna sequence is repeated in the genome and some of its locations are different in various wild-type strains. this dna element exhibits structural similarities with the yeast solo delta, sigma or tau elements. | 1990 | 1975665 |
| a site-specific deletion in mitochondrial dna of podospora is under the control of nuclear genes. | in the filamentous fungus podospora anserina, the association of two nuclear genes inevitably leads to a "premature death" phenotype consisting of an early end of vegetative growth a few days after ascospore germination. mycelia showing this phenotype contain a mitochondrial chromosome that always bears the same deletion. one of the break points is exactly at the 5' splice site of a particular mitochondrial intron, suggesting that the deletion event could result from molecular mechanisms also in ... | 1991 | 2023905 |
| insertion of an lrdna gene fragment and of filler dna at a mitochondrial exon-intron junction in podospora. | a rearrangement of the mitochondrial genome of a long lived mutant of podospora anserina is presented. it consists in the insertion of 191 bp of the lrdna gene (coding for the large ribosomal rna) at the junction between exon1 and intron alpha of gene co1 (coding for subunit 1 of cytochrome oxidase). this insertion is accompanied by a 53 bp deletion of the junction and the presence of extra a and t nucleotides at both sides of the inserted sequence. we discuss possible mechanisms of production o ... | 1990 | 2156230 |
| mitochondrial dna sequence analysis of the cytochrome oxidase subunit ii gene from podospora anserina. a group ia intron with a putative alternative splice site. | a 5 kb region of the 95 kb mitochondrial genome of podospora anserina race s has been mapped and sequenced (1 kb = 10(3) base-pairs). this dna region is continuous with the sequence for the nd4l and nd5 gene complex in the accompanying paper. we show that this sequence contains the gene for cytochrome oxidase subunit ii (coii). this gene is 4 kb in length and is interrupted by a subgroup ib intron (1267 base-pairs (bp) in length) and a subgroup ia intron (1992 bp in length). this group ia intron ... | 1990 | 2157023 |
| self-splicing of the mobile group ii intron of the filamentous fungus podospora anserina (coi i1) in vitro. | the first intron of the mitochondrial gene coding for cytochrome oxidase subunit i (coi i1) of podospora anserina can undergo self-splicing in vitro at high concentrations of nh4cl or kcl. under these conditions cleavage at the 5' splice junction takes place without branch formation probably via hydrolysis by water or oh- and the intron is released in a linear form. in vitro transcripts that contain mutated introns with large deletions in nonconserved domain iv comprising greater than 50% of the ... | 1990 | 2162769 |
| dna sequence analysis of the mitochondrial nd4l-nd5 gene complex from podospora anserina. duplication of the nd4l gene within its intron. | a 15 kb region of the 100 kb mitochondrial genome of podospora anserina has been mapped and sequenced (1 kb = 10(3) base-pairs). the genes for nd4l and nd5 are identified as contiguous genes with overlapping termination and initiation codons. in race a (101 kb) the gene for nd4l (4.3 kb) has a gene duplication within an intron including a second subgroup ic intron. race s (95 kb) lacks this second gene complex. each intron has the identical 5' exon boundary. secondary structure analysis showed t ... | 1990 | 2319602 |
| the complete dna sequence of the mitochondrial genome of podospora anserina. | the complete 94,192 bp sequence of the mitochondrial genome from race s of podospora anserina is presented (1 kb = 10(3) base pairs). three regions unique to race a are also presented bringing the size of this genome to 100,314 bp. race s contains 31 group i introns (33 in race a) and 2 group ii introns (3 in race a). analysis shows that the group i introns can be categorized according to families both with regard to secondary structure and their open reading frames. all identified genes are tra ... | 1990 | 2357736 |
| development of a genetic transformation system for histoplasma capsulatum: complementation of uracil auxotrophy. | we have developed a simple and efficient transformation system for the dimorphic fungus histoplasma capsulatum. mutants of h. capsulatum defective in orotidine-5'-monophosphate pyrophosphorylase were transformed to prototrophy by the cloned ura5 gene of the filamentous fungus podospora anserina. abortive and mitotically stable transformants were obtained. the stable transformants had integrated copies of the plasmid, some in tandem head-to-tail orientation. free plasmid identical to the transfor ... | 1990 | 2381417 |
| genetic and molecular analysis of a long-lived strain of podospora anserina. | a genetic and molecular analysis of a long-lived strain of podospora anserina, mn19, was undertaken to detect mutations in genes responsible for senescence. in crosses between mn19 and wild type about 15% of the progeny were long-lived, regardless of the female parent. molecular analysis of the long-lived progeny showed that none of the strains inherited a mtdna rearrangement characteristic of the mn19 parent. instead, all long-lived strains initially inherited wild-type mtdna. over time the mtd ... | 1990 | 2397883 |
| a dna polymerase activity with characteristics of a reverse transcriptase in podospora anserina. | in the filamentous ascomycete fungus podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. during senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. in this paper, we report our studies on nuclear-mitochondrial extracts for the presence of rna-dependent dna polymerase activity (reverse transcriptase). in so-called middle aged mycelia of race a, we detected a dna polymerase possessing properti ... | 1986 | 2450680 |
| an additional class ii intron with homology to reverse transcriptase in rapidly senescing podospora anserina. | senescence in podospora anserina is maternally inherited and the parameters of senescence are race specific. we have compared the restriction enzyme fragment maps of race a, the most rapidly senescing race, with race s and have found three inserts in race a which are not present in race s mitochondrial dna. fragment a was mapped and found to be located downstream of the so-called alpha sendna, a class ii intron, near the 5' end of the coi gene, separated from alpha sendna by two class i introns. ... | 1986 | 2452024 |
| dna sequence, structure, and phylogenetic relationship of the small subunit rrna coding region of mitochondrial dna from podospora anserina. | dna sequence analysis and the localization of the 5' and 3' termini by s1 mapping have shown that the mitochondrial (mt) small subunit rrna coding region from podospora anserina is 1980 bp in length. the analogous coding region for mt rrna is 1962 bp in maize, 1686 bp in saccharomyces cerevisiae, and 956 bp in mammals, whereas its counterpart in escherichia coli is 1542 bp. the p. anserina mt 16s-like rrna is 400 bases longer than that from e. coli, but can be folded into a similar secondary str ... | 1989 | 2494352 |
| dna sequence and secondary structures of the large subunit rrna coding regions and its two class i introns of mitochondrial dna from podospora anserina. | dna sequence analysis has shown that the gene coding for the mitochondrial (mt) large subunit ribosomal rna (rrna) from podospora anserina is interrupted by two class i introns. the coding region for the large subunit rrna itself is 3715 bp and the two introns are 1544 (r1) and 2404 (r2) bp in length. secondary structure models for the large subunit rrna were constructed and compared with the equivalent structure from escherichia coli 23s rrna. the two structures were remarkably similar despite ... | 1989 | 2494353 |
| recombination and replication of plasmid-like derivatives of a short section of the mitochondrial chromosome of neurospora crassa. | the 21-kbp mitochondrial chromosome of the stp-ruv strain of neurospora crassa undergoes regional amplification yielding plasmid-like supercoiled circles varying in size from subunit length to very high multimers. a comparison of the base sequence of the five plasmids studied, with the region of the chromosome from which they were derived, indicated that the amplified chromosomal segments were determined by a recombination-excision process near or within two structurally distinctive regions. one ... | 1989 | 2524421 |
| multipartite structure of mitochondrial dna in a fungal longlife mutant. | rearrangements in the mitochondrial genome of the longlife mutant ex1 of the ascomycete podospora anserina have led to a heterogeneous population of subgenomic molecules. the restriction maps of individual subcircles were established using overlapping recombinant lambda phages isolated from an ex 1 mtdna/embl3 library. the formation of the subcircles and the resulting multipartite organization of the ex1 mtdna are discussed. | 1989 | 2524849 |
| dna sequence analysis of the 24.5 kilobase pair cytochrome oxidase subunit i mitochondrial gene from podospora anserina: a gene with sixteen introns. | the dna sequence of a 26.7 kilobase pair (10(3) base pairs = 1 kb) region of the mitochondrial genomes of races s and a from podospora anserina was determined. within this region, the 24.5 kb cytochrome oxidase subunit i gene was located and its exon sequences determined by computer analysis comparisons with other fungal genes. the podospora coi gene was interrupted by two group ii introns (one in race s) and fourteen group i introns ranging in size from about 2.2 kb to 404 bp. earlier studies o ... | 1989 | 2558809 |
| transformation by integration in podospora anserina. iii. replacement of a chromosome segment by a two-step process. | we have developed in podospora anserina a two-step procedure for dna sequence replacement through transformation which might be applicable to other filamentous fungi. targeting of transforming dnas to their homologous locus is achieved provided a cosmid vector is used. southern blot analysis of genomic dnas from a set of transformants is presented. the data confirm that cosmids integrate into the chromosome through mostly homologous recombination which leads to a duplicated sequence separated by ... | 1989 | 2575706 |
| dna sequence analysis of the apocytochrome b gene of podospora anserina: a new family of intronic open reading frame. | the 5,969 bp (base pair) dna sequence of the apocytochrome b mitochondrial (mt) gene of race a podospora anserina was located in a 8.5 kbp region. this gene contained a 2,499 bp subgroup ib and a 1,306 bp subgroup id intron as well as a 990 bp subgroup ib intron which is present in race a but not race s. the large subgroup ib intron and the race a specific ib intron both contained potential alternate splice sites which brought their open reading frames into phase with their upstream exon sequenc ... | 1989 | 2611913 |
| heat shock at an elevated temperature improves transformation efficiency of protoplasts from podospora anserina. | we have developed an improved transformation procedure for the filamentous fungus podospora anserina. this procedure is based on the observation that a heat shock at an elevated temperature (48 degrees c) improves the competence of p. anserina protoplasts for transformation 5- to 10-fold. this is observable only if the heat shock is applied before the addition of transforming dna. an increase in competence is observed immediately after the heat shock, and heat-shocked cells are still competent a ... | 1989 | 2621442 |
| transformation in fungi. | transformation with exogenous deoxyribonucleic acid (dna) now appears to be possible with all fungal species, or at least all that can be grown in culture. this field of research is at present dominated by saccharomyces cerevisiae and two filamentous members of the class ascomycetes, aspergillus nidulans and neurospora crassa, with substantial contributions also from fission yeast (schizosaccharomyces pombe) and another filamentous member of the class ascomycetes, podospora anserina. however, tr ... | 1989 | 2651864 |
| the ura5 gene of the ascomycete sordaria macrospora: molecular cloning, characterization and expression in escherichia coli. | we cloned the ura5 gene coding for the orotate phosphoribosyl transferase from the ascomycete sordaria macrospora by heterologous probing of a sordaria genomic dna library with the corresponding podospora anserina sequence. the sordaria gene was expressed in an escherichia coli pyre mutant strain defective for the same enzyme, and expression was shown to be promoted by plasmid sequences. the nucleotide sequence of the 1246-bp dna fragment encompassing the region of homology with the podospora ge ... | 1989 | 2663654 |
| mitochondrial group i introns from the filamentous fungus podospora anserina code for polypeptides related to maturases. | | 1987 | 2819827 |
| the onset of senescence is affected by dna rearrangements of a discontinuous mitochondrial gene in podospora anserina. | mapping and transcription studies have revealed that in podospora anserina the causative agent of senescence, a mitochondrial plasmid (pldna), is identical with intron 1 of the discontinuous gene for cytochrome-c-oxidase subunit 1 (coi), which is 2 kpb from the discontinuous gene for cytochrome b (cytb). a mitochondrial mutant (ex1) devoid of the coi, but not of the cytb gene provides longevity. a molecular model for the onset of senescence is presented. | 1985 | 2836091 |
| mitochondrial dna rearrangements are correlated with a delayed amplification of the mobile intron (pldna) in a long-lived mutant of podospora anserina. | a new long-lived mutant of podospora anserina has been isolated and characterized. its longevity is maternally inherited as revealed by reciprocal crosses. a molecular analysis resulted in the identification of an amplified dna species (designated pal2-1) with homology to mitochondrial dna (mtdna). the presence of this dna species is correlated with mtdna rearrangements and a delayed amplification of the mobile intron (pldna). | 1989 | 2911274 |
| sequence analysis of mitochondrial dna from podospora anserina. pervasiveness of a class i intron in three separate genes. | a 48 kb region of the 95 kb mitochondrial genome of podospora anserina has been mapped and sequenced (1 kb = 10(3) base-pairs). the dna sequence of the genes for nd2, 3, 4, atpase 6 and urfc are presented here. as in neurospora crassa, the nd2 and 3 genes consist of a unit separated by one taa stop codon. nd3, 4 and atpase 6 are interrupted by class i introns. all three introns are remarkably similar in the c-domain of their secondary structure, sufficient enough to designate them as new subgrou ... | 1988 | 2975708 |
| cloning and characterization of the rdna repeat unit of podospora anserina. | dna coding for ribosomal rna in podospora anserina has been cloned and was found as a tandemly repeated 8.3 kb sequence. the cloned rdna was characterized by restriction endonuclease mapping. the location of 5.8s, 18s and 28s rrna coding regions was established by dna-rna hybridization and s1 nuclease mapping. the organization of p. anserina rrna genes is similar to that of neurospora crassa and aspergillus nidulans. the rdna unit does not contain the sequence coding for 5s rna. | 1985 | 2987647 |
| excision-amplification of mitochondrial dna during senescence in podospora anserina. dna sequence analysis of three unique "plasmids". | during senescence in the filamentous fungus podospora anserina, specific regions of the mitochondrial genome, termed sendna are excised, ligated and amplified. we have cloned in their entirety three such autonomously replicating plasmids, alpha, beta and epsilon sendna. none of these plasmids displayed cross-hybridization nor did we detect any significant dna homology by computer analysis. the complete dna sequence of the 2.5 kb alpha, the 5.5 kb epsilon and about 3.4 kb of the 9.8 kb beta sendn ... | 1985 | 2997455 |
| the mitochondrial urf1 gene in neurospora crassa has an intron that contains a novel type of urf. | in neurospora crassa, a 2670 base-pair segment of the mitochondrial dna was sequenced including a gene homologous to the mammalian urf1 that was recently shown to encode a subunit of the respiratory chain nadh dehydrogenase complex. urf1 of n. crassa is interrupted by an intron of 1118 base-pairs that divides the protein-coding sequence into two exons of 636 and 480 base-pairs length, respectively. the deduced urf1 polypeptide of 371 residues was aligned with that of other eukaryotes, revealing ... | 1985 | 3003362 |
| podospora anserina mutant defective in protoperithecium formation, ascospore germination, and cell regeneration. | a mutant (modx) was selected on the basis of the suppression of self-lysis due to a recessive mutation (modb). modx, a dominant mutation, reduced hyphal branching from nonapical cells, abolished protoperithecium formation, and induced the death of stationary cells only when these were isolated to obtain further development. mutant ascospores, formed in the fruiting bodies which occasionally occur under specific conditions (32 degrees c on starved medium), showed a delay in the germination proces ... | 1979 | 118158 |
| a constitutive mutation in a posttranscriptional regulator gene for a phenoloxidase and proteases in podospora anserina. | a recessive mutation in the gene mod-2 results in the synthesis at low temperatures of a phenoloxidase and an arrest of growth, reversible by beta-phenyl-pyruvic acid, a protease inhibitor. phenoloxidase synthesis is 5-fluorouracil resistant and cycloheximade sensitive. suppression of both cold sensitivity and phenoloxidase synthesis by common factors (higher nh4+ concentrations or mutations) suggests that the protease, suspected to be responsible for cold sensitivity, also arises from preexisti ... | 1976 | 137717 |
| the phenoloxidases of the ascomycete podospora anserina. xi. the state of copper of laccases i, ii and iii. | 1. laccases i, ii and iii were (ec 1.14.18.1) prepared from the mycelium of the ascomycete podospora anserina. the tetrameric laccase i(mol. wt 340 000, 16 copper atoms) and the monomeric laccases ii and ii (mol. wt 80 000, 4 copper atoms) have been studied by optical absorption-, circular dichroism-(cd)and electron paramagnetic resonance spectroscopy (epr). 2. the visible and near ultraviolet difference absorption spectrum, which is apparently identical for all three laccases, shows two maxima ... | 1975 | 166666 |
| mitochondrial dna from podospora anserina. i. isolation and characterization. | mitochondrial (mt) dna from podospora anserina was isolated and characterized with respect to density in cscl, contour length and endonuclease restriction enzymes. the density of mt dna for four races examined was 1.694 g/cm3, compared with 1.712 g/cm3 for nuclear dna. extraction in the presence of a nuclease inhibitor, aurintricarboxylic acid and isolation in dapi cscl gradients allowed us to isolate high molecular weight dna. mt dna isolated by total dna extraction contained ca. 1% of circular ... | 1979 | 286867 |
| mitochondrial dna from podospora anserina. ii. properties of mutant dna and multimeric circular dna from senescent cultures. | mitochondrial (mt) dna from mitochondrial mutants of race s podospora anserina and from senescent cultures of races s and a was examined. in mutants, we observed that fewer full length circles (31 mu) were present; instead, smaller circles characteristic for each mutant studied were found. eco r1 digestion of these mutant mtdnas indicated that in certain mutants, although specific fragments were absent, the total molecular weight of the fragments was not much different than wild-type. the proper ... | 1979 | 286868 |
| chromosomal and extrachromosomal control of senescence in the ascomycete podospora anserina. | in podospora anserina senescence leading to cellular death occurs regularly after prolonged vegetative propagation. however, the life span of this ascomycete may be extended by various means: 1. mutations in a least 8 morphogenetic genes belonging to 4 linkage groups postpone drastically or even prevent in certain pairwise combinations (e.g. i viv) the onset of senescence. 2. inhibitors of mt dna and of mitochondrial protein synthesis show a life prolonging effect when added in low concentration ... | 1979 | 288965 |
| the phenoloxidases of the ascomycete podospora anserina. xiii. action and interaction of genes controlling the formation of laccase. | 1. eight mutants were isolated following mutagen treatment which are deficient in laccase formation. seven of these had a pleiotropic effect and exhibited defects in growth rate and in mycelial and sexual morphology. 2. by means of tetrad analysis the mutations were assigned to 6 loci. three mutations were in the incolora locus, the others were non-allelic. only two of these loci were closely linked. 3. all genes exhibit numerous interactions. these concern the morphological expression of the la ... | 1977 | 414070 |
| an attempt to establish a model for the rhythmic phenomena affecting the growth or sporulation of certain fungi. | a simple two-variable mathematical model is proposed, able to acount for periodic variations relating to growth in podospora anserina and fructification in aspergillus niger. this basic model is open to generalization. | 1979 | 520098 |
| antioxidants prolong life span and inhibit the senescence-dependent accumulation of fluorescent pigment (lipofuscin) in clones, of podospora anserina. | culture of clones of podospora anserina s+ with either nordihydroguaiaretic acid or reduced glutathione (gsh) at concentrations that were not inhibitory to growth significantly prolonged the average time to onset of senscence. gsh also prolonged the average time to onset of clonal death. the specific concentration of chloroform-methanol soluble fluorescent pigment was larger in senescent than in pre-senescent cells. the pigment exhibited fluorescence excitation and emission spectra and fluoresce ... | 1978 | 672262 |
| evidence for plasmid like dna in a filamentous fungus, the ascomycete podospora anserina. | the existence of plasmid like dna was demonstrated in senescent mycelia of podospora anserina (strain s) by biophysical and electronmicroscopic methods. according to their contour length of about 1.4 and 2.7 micron respectively the molecular weight for the monomer is in the range of 3.10(6). | 1978 | 683172 |
| ribosomal suppressors and antisuppressors in podospora anserina: resistance to cycloheximide. | informational suppressors and antisuppressors have been previously isolated in podospora anserina, and a range of exclusively genetic arguments have led to the assumption that they correspond to ribosomal mutations. an in vivo and in vitro comparison of the effect of the ribosomal inhibitor cycloheximide on wildtype and mutant strains described in this paper confirms the ribosomal hypothesis for at least some mutants. indeed, the four mutants in the as3 gene were cycloheximide resistant, and the ... | 1977 | 893344 |
| oxidative stress and ageing in the fungus podospora anserina. | the ageing phenomenon exhibited by the ascomycetous fungus podospora anserina can be either delayed or induced by either different carbon sources or effectors. as these effects seem to have analogy to catabolite-repression of respiratory genes, experiments concerning respiratory functions have been carried out. ageing is parallelled by switching from cytochrome c-oxidase-mediated respiration to alternative, cyanide-resistant respiration for reasons still unknown. the latter is always accompanied ... | 1992 | 1331631 |
| self-splicing of a mitochondrial group i intron from the cytochrome b gene of the ascomycete podospora anserina. | we have shown that the second intron of the podospora mitochondrial gene coding for cytochrome b (cytb 12) splices autocatalytically, using in vitro transcripts generated from the t7 promoter. the reaction takes place at 37 degrees c in the presence of 50 mm tris-hcl ph 7.5, 60 mm mgcl2 and 1 mm gtp but shows a low efficiency even at high kcl concentrations of up to 1.2 m. under these conditions, intron bi2 follows the conventional pathway of group i splicing, and all characteristic products, wi ... | 1992 | 1376408 |
| the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase. | a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ... | 1992 | 1421143 |
| the pyr1 gene of the plant pathogenic fungus colletotrichum graminicola: selection by intraspecific complementation and sequence analysis. | a spontaneous uridine-requiring auxotroph of colletotrichum graminicola was recovered by selection for resistance to 5-fluoro-orotic acid. the auxotroph lacked orotate phosphoribosyl transferase (oprtase) and was complemented with a clone from a cosmid library of c. graminicola dna. a 3.1 kb hindiii-sali fragment was subcloned from the cosmid and it could efficiently transform the auxotrophic strain to uridine prototrophy and integrate by site-specific recombination. this dna fragment contains a ... | 1992 | 1435732 |
| the linear mitochondrial plasmid pal2-1 of a long-lived podospora anserina mutant is an invertron encoding a dna and rna polymerase. | the molecular characterization of an additional dna species (pal2-1) which was identified previously in a long-lived extrachromosomal mutant (al2) of podospora anserina revealed that this element is a mitochondrial linear plasmid. pal2-1 is absent from the corresponding wild-type strain, has a size of 8395 bp and contains perfect long terminal inverted repeats (tirs) of 975 bp. exonuclease digestion experiments indicated that proteins are covalently bound at the 5' termini of the plasmid. two lo ... | 1992 | 1473181 |
| two allelic genes responsible for vegetative incompatibility in the fungus podospora anserina are not essential for cell viability. | vegetative incompatibility is a lethal reaction that destroys the heterokaryotic cells formed by the fusion of hyphae of non-isogenic strains in many fungi. that incompatibility is genetically determined is well known but the function of the genes triggering this rapid cell death is not. the two allelic incompatibility genes, s and s, of the fungus podospora anserina were characterized. both encode 30 kda polypeptides, which differ by 14 amino acids between the two genes. these two proteins are ... | 1991 | 1886611 |
| cloning and sequencing of ura10, a second gene encoding orotate phosphoribosyl transferase in saccharomyces cerevisiae. | orotate phosphoribosyl transferase (op-rtase) catalyses the transformation of orotate to omp in the pyrimidine pathway. in the yeast saccharomyces cerevisiae, the ura5 gene is known to encode this enzyme activity. in this paper we present the cloning and sequencing of a yeast gene, named ura10, encoding a second oprtase enzyme. comparison of the predicted amino acid sequences between ura5 and ura10 genes shows more than 75% similarity. these sequences have also been compared to those of escheric ... | 1990 | 2182197 |
| cloning and structure of the pyre gene of lactobacillus plantarum ccm 1904. | the pyre gene of lactobacillus plantarum ccm 1904, coding for the orotate phosphoribosyl transferase involved in the pyrimidine biosynthetic pathway, was cloned in escherichia coli and sequenced. the predicted polypeptide sequence extending over 212 amino acids (mw 22,690) was compared to those of e. coli and to those of lower eukaryotes (saccharomyces cerevisiae, podospora anserina, sordaria macrospora, dictyostelium discoideum). important conserved stretches were revealed, implying that these ... | 1990 | 2210335 |
| a mitochondrial dna rearrangement and three new mitochondrial plasmids from long-lived strains of podospora anserina. | the excision-junction sites of a mtdna rearrangement of a long-lived strain of podospora anserina, mn19, were cloned and sequenced. analysis of sequence and hybridization data lead to the conclusion that the mn19 mtdna consists of two nonoverlapping circular molecules. three plasmids, lmt-2, lmt-3, and lmt-4, cloned from long-lived progeny of crosses between the mn19 strain and wild type were cloned and sequenced. these plasmids share features and excision-junction sites with previously describe ... | 1990 | 2270228 |
| detection of a protein encoded by a class ii mitochondrial intron of podospora anserina. | in the filamentous fungus podospora anserina, the amplification as circular dna molecules of the first intron (intron alpha) of the co1 mitochondrial gene, encoding the cytochrome oxidase subunit 1, is known to be strongly associated with aging of strains. in this study we have attempted to detect the protein potentially encoded by the open reading frame (orf) contained in this intron. this was done by the western blot technique using specific antisera raised against three polypeptides encoded b ... | 1990 | 2277641 |
| excision-amplification of mitochondrial dna during senescence in podospora anserina. a potential role for an 11 base-pair consensus sequence in the excision process. | three novel mitochondrial excision-amplification plasmids of podospora anserina were identified and the excision-junction sites on the mitochondrial genome determined. all three plasmids were at least partially derived from a common region of the mitochondrial genome termed ecori-7 (e7). the entire 5651 base-pair sequence of e7 is presented. included within this sequence are the e7-specific excision-junction sites of these novel plasmids, the localizations of nine trna genes, and the localizatio ... | 1987 | 2448479 |
| structure and expression of the ura5 gene of saccharomyces cerevisiae. | the ura5 gene of saccharomyces cerevisiae encodes orotate phosphoribosyl transferase (ec 2.4.2.10; oprtase) which catalyses the transformation of orotate to omp in the pyrimidine pathway. we present in this paper the cloning and the sequencing of this gene, the last in the yeast pyrimidine pathway to be cloned. we have deduced the protein sequence of the oprtase of s. cerevisiae from the dna sequence and compared it to that of escherichia coli, podospora anserina and dictyostelium discoideum. so ... | 1989 | 2651891 |
| a novel family of mitochondrial plasmids associated with longevity mutants of podospora anserina. | we have identified a novel family of plasmids, each containing very short monomeric units, in podospora anserina longevity mutants. these plasmids, termed small mitochondrial dnas (smt-dnas), are derived from a highly ordered 368-base pair region of the mitochondrial genome. a total of five direct repeat sequences and seven significant regions of dyad symmetry (i.e. palindromes) were found within a 434-base pair mitochondrial sequence, which includes this 368-base pair region. mitochondrial dna ... | 1987 | 3029071 |
| dna sequence and organization of the mitochondrial nd1 gene from podospora anserina: analysis of alternate splice sites. | earlier, we reported that the nd1 mitochondrial gene of podospora anserina is mosaic, containing at least three class i introns. we have now completed the sequence of the nd1 gene and have determined that it contains four class i introns of 1,820, 2,631, 2,256 and 2,597 bp with the entire gene complex containing 10,505 bp, only 1,101 of which are exon sequences. introns 1 and 3 appear to be related in that their open reading frames (orfs) exhibit extensive amino acid sequence similarity and like ... | 1988 | 3197134 |
| senescence in podospora anserina: a possible role for nucleic acid interacting proteins suggested by the sequence analysis of a mitochondrial dna region specifically amplified in senescent cultures. | in podospora anserina, the phenomenon of senescence was previously shown to be correlated with the presence of a senescence-specific dna (sen-dna) resulting from the amplification of some regions (alpha, beta, gamma, epsilon) of the mitochondrial chromosome. the beta region gives rise to sen-dnas with variable sizes and junctions which share a 1,100-bp common sequence. here we report the complete nucleotide sequence of one 4-kb beta sen-dna. included in the sequence are a large part of the first ... | 1988 | 3246349 |
| non-homologous integration of transforming vectors in the fungus podospora anserina: sequences of junctions at the integration sites. | transformation of the ura5-6 mutant strain of podospora anserina with a recombinant vector carrying the ura5+ gene often results in the integration of the transforming plasmid by non-homologous recombination outside of the genomic ura5 locus. to investigate the mechanism of such integration, we rescued the integrated plasmid from three transformants. in two cases, the rescued plasmid was highly altered compared with the original transforming vector. we cloned the junctions between plasmidic dna ... | 1988 | 3246350 |
| identification of two genes controlling kasugamycin resistance in the filamentous fungus podospora anserina. | | 1988 | 3417144 |
| genetics of translational fidelity in podospora anserina: are all the genes involved in this ribosomal function identified? | su12-1 and su12-2 are two ribosomal suppressor mutations previously described in the fungus podospora anserina. revertants were isolated on the criteria of either improved growth at 27 degrees c (for su12-1) or suppression of the paromomycin hypersensitivity (for su12-2). among 45 mutations lying outside the su12 locus, only one was found which defines a new antisuppressor locus, as9. about 3/4 of these mutations are antisuppressor mutations localized in the previously identified as6 and as7 gen ... | 1986 | 3442829 |
| plasmids of mitochondrial origin in senescent mycelia of podospora curvicolla. | podospora curvicolla displays symptoms of senescence similar but not quite identical to those reported for podospora anserina. in podospora curvicolla single hyphae may escape from death leading to a new growth front and consequently to a mode of growth characterized by alternating phases of growth and non-growth. restriction analyses and hybridization experiments have revealed that the podospora curvicolla type of senescence is correlated with plasmids originating from amplification of a single ... | 1986 | 3447738 |
| positive screening and transformation of ura5 mutants in the fungus podospora anserina: characterization of the transformants. | to develop a transformation system in the filamentous fungus podospora anserina we have selected ura5 mutants deficient in orotidylic acid pyrophosphorylase using a positive screening. these mutants could be transformed to prototrophy by an hybrid vector carrying the ura5 gene of this organism. the properties of the transformants have been analysed. in most cases integration of the transforming vector occurred outside the ura5 locus and frequently repeated tandem copies of the vector were found. ... | 1986 | 3447739 |
| nonintegrative transformation in the filamentous fungus podospora anserina: stabilization of a linear vector by the chromosomal ends of tetrahymena thermophila. | the effect of the chromosomal ends of tetrahymena thermophila on the stability of linear transforming molecules in the filamentous fungus podospora anserina was tested. a derivative of an integrative vector for this fungus has been constructed, so that after linearization, the ends of the plasmid are the telomeric sequences of t. thermophila. after transformation, this linear molecule was maintained as an extrachromosomal plasmid with no integrated copies in about 50% of the transformants. under ... | 1987 | 3600642 |
| the ura5 gene of the filamentous fungus podospora anserina: nucleotide sequence and expression in transformed strains. | we have sequenced the ura5 gene of the filamentous fungus podospora anserina. the deduced sequence for the orotidylic acid pyrophosphorylase (ompppase) has been compared with the escherichia coli enzyme which is the only known sequence for this enzyme. this comparison shows extensive blocks of homology. the expression of the ura5 gene has been studied in a ura5 mutant which has been transformed by a recombinant plasmid carrying the ura5 gene. we observed that strains carrying integrated multicop ... | 1987 | 3609748 |
| a podospora anserina longevity mutant with a temperature-sensitive phenotype for senescence. | a podospora anserina longevity mutant was identified with a temperature-sensitive phenotype for senescence. this mutant, termed ts1, grew for over 3 m at 27 degrees c, but when shifted to 34 degrees c, it underwent senescence between 10 and 18 cm. a previously described senescence-associated plasmid, alpha sendna, derived from the mitochondrial genome, was not detected in ts1 at 27 degrees c but was present in senescent cultures at 34 degrees c. a similar result was observed in progeny strains o ... | 1987 | 3670311 |
| at least seven ribosomal proteins are involved in the control of translational accuracy in a eukaryotic organism. | in the filamentous fungus podospora anserina, ribosomal proteins of 60 mutants impaired in the control of translational fidelity have been submitted to electrophoretic analysis. the "four corners" system combining four different two-dimensional polyacrylamide gel electrophoretic systems has been used. an altered electrophoretic pattern has been observed for 12 mutants. in mutants su3, su12 and su11 (decreased translational fidelity), proteins s1, s7 and s8, respectively, are altered. for as muta ... | 1986 | 3795267 |
| podospora anserina does not senesce when serially passaged in liquid culture. | a procedure was developed for the prolonged growth of the ascomycete fungus podospora anserina in liquid culture to determine the effects of such growth on the senescence phenotype. senescence in p. anserina, which is maternally inherited and associated with the excision and amplification of specific mitochondrial plasmids, occurs when this species is grown on solid medium. in two independent experiments no evidence of senescence was observed as mycelia were serially passaged in liquid culture. ... | 1987 | 3804968 |
| identification of the structural gene for the s9 ribosomal protein in the fungus podospora anserina: a new protein involved in the control of translational accuracy. | as9-1 was isolated as a mutation restoring growth in a strain carrying the ribosomal mutation su12-1. the as9-1 mutation confers a weak antisuppressor effect and a low level of resistance to paromomycin. two-dimensional polyacrylamide gel electrophoresis patterns of the ribosomal proteins from as9-1 strains show an altered s9 protein which is more basic than the wild-type form. the presence of the two forms of the protein (wild-type and mutant) in heterocaryotic strains strongly suggests that as ... | 1985 | 3861930 |
| cloning of opal suppressor trna genes of a filamentous fungus reveals two trnaseruga genes with unexpected structural differences. | the informational suppressors su4-1 and su8-1 of podospora anserina were isolated by transformation of schizosaccharomyces pombe uga mutants. the dna sequence revealed that they were opal (uga) suppressor trnas. wild-type alleles were also isolated by hybridization. the dna sequence showed that they both encode species of trnaseruga. the gene su8 has an 18-bp intervening sequence and its primary sequence is very different from that of su4. | 1985 | 3937728 |
| analysis of class i introns in a mitochondrial plasmid associated with senescence of podospora anserina reveals extraordinary resemblance to the tetrahymena ribosomal intron. | recently, the nucleotide sequences for three "mitochondrial plasmids" associated with senescence of podospora anserina were determined (cummings et al. 1985). one of these sequences, corresponding to the plasmid termed epsilon sendna, contains three class i introns, all within a protein coding sequence equivalent to the mammalian "urf1" gene. here, we present primary and secondary structure analyses for two of these introns as well as a partial analysis for the third, which extends beyond the dn ... | 1985 | 3940064 |
| different alterations of the ribosomal protein s7 lead to opposite effects on translational fidelity in the fungus podospora anserina. | in the fungus podospora anserina, the su12-1 mutation was previously found to decrease translational accuracy and to alter the ribosomal protein s7. the mutant protein is more basic than the wild type. among the revertants of the two ribosomal mutations su12-1 and su12-2, 29 contained a second mutation very closely linked to su12. biochemical analysis of these revertants by functional poly(u) tests and electrophoretical study of the ribosomes led to two conclusions. first, some revertant strains ... | 1986 | 3949806 |
| ribosomal suppressors in podospora anserina: evidence for two new loci by means of a new screening procedure. | | 1985 | 3996914 |
| the mitochondrial genome of the fission yeast schizosaccharomyces pombe. the cytochrome b gene has an intron closely related to the first two introns in the saccharomyces cerevisiae cox1 gene. | the dna sequence of the cob region of the schizosaccharomyces pombe mitochondrial dna has been determined. the cytochrome b structural gene is interrupted by an intron of 2526 base-pairs, which has an open reading frame of 2421 base-pairs in phase with the upstream exon. the position of the intron differs from those found in the cob genes of saccharomyces cerevisiae, aspergillus nidulans or neurospora crassa. the sch. pombe cob intron has the potential of assuming an rna secondary structure almo ... | 1985 | 4046021 |
| incompatibility in the fungus podospora anserina. are the mutations abolishing the incompatibility reaction ribosomal mutations? | | 1973 | 4126298 |