| microbial transformations of natural antitumor agents: o-demethylation of vindoline by sepedonium chrysospermum. | vindoline (1a) was transformed to o-demethylvindoline (1b) by sepedonium chrysospermum atcc 13378 in 33% yield. thin-layer and high-pressure liquid chromatographic methods distinguished 1b from previously reported microbial metabolites of vindoline. the structural proof of 1b was based on its mass spectral fragmentation pattern and on its chemical and nuclear magnetic resonance spectral properties. | 1978 | 568911 |
| dna probe for the identification of histoplasma capsulatum. | a 1.85-kilobase hindiii nuclear dna probe from histoplasma capsulatum g217b detected polymorphic restriction fragments within whole-cell dna from different clinical isolates of h. capsulatum, consistent with the previous system of classification. the probe failed to hybridize to dna from blastomyces dermatitidis, candida spp., saccharomyces cerevisiae, sepedonium chrysospermum, and chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent t ... | 1989 | 2573616 |
| production of a monoclonal antibody specific to the genus trichoderma and closely related fungi, and its use to detect trichoderma spp. in naturally infested composts. | studies of the interactions between hyperparasitic fungi and their hosts are severely hampered by the absence of methods that allow the unambiguous identification of individual genera in complex environments that contain mixed populations of fungi, such as soil or compost. this study details the development of a monoclonal antibody (mf2) that allows the detection and recovery of trichoderma spp. in naturally infested composts, and the visualization of hyperparasitic strains of trichoderma during ... | 2002 | 11988501 |
| chrysosporide, a cyclic pentapeptide from a new zealand sample of the fungus sepedonium chrysospermum. | a new cyclic pentapeptide, chrysosporide (1), was isolated from a new zealand sample of the mycoparasitic fungus sepedonium chrysospermum by bioactivity-guided fractionation. the planar structure was deduced by detailed spectroscopic analysis, and the absolute configurations of the amino acid residues were defined by marfey's method. as both enantiomers of leu occurred in chrysosporide, molecular mechanics calculations were applied to the analysis to distinguish between the possible structural i ... | 2006 | 17067166 |
| production of sepedonin by sepedonium chrysospermum nt-1 in submerged culture. | strains of sepedonium chrysospermum and the anamorph strain of hypomyces chrysospermus (congruent with apiocrea chrysosperma) were isolated and purified from parasitic filamentous fungi on the fruiting bodies of boletaceae, such as the gyroponus and suillus genera in japan, and identified from formations of conidia and chlamydospores. it is known that these strains produce sepedonin. s. chrysospermum nt-1 strain was selected from these strains and isolated. as the optimum medium (cy-1 medium), 0 ... | 2006 | 17095998 |
| phylogenetic position of the yeast-like symbiotes of tagosodes orizicolus (homoptera: delphacidae) based on 18s ribosomal dna partial sequences. | tagosodes orizicolus muir (homoptera: delphacidae), the endemic delphacid species of tropical america carries yeast-like symbiotes (yls) in the abdominal fat bodies and the ovarial tissues, like other rice planthoppers of asia. these yls are obligate symbiotes, which are transmitted transovarially, and maintain a mutualistic relationship with the insect host. this characteristic has made in vitro culture and classification of yls rather difficult using conventional methods. nevertheless, microor ... | 2004 | 17361570 |
| evolving trends in the dereplication of natural product extracts. 2. the isolation of chrysaibol, an antibiotic peptaibol from a new zealand sample of the mycoparasitic fungus sepedonium chrysospermum. | by the application of an hplc bioactivity profiling/microtiter technique in conjunction with capillary nmr instrumentation and access to the antimarin database the conventional evaluation/isolation dereplication/characterization procedures can be dramatically truncated. this approach is illustrated using the isolation of a new peptaibol, chrysaibol (1), from a new zealand isolate of the mycoparasitic fungus sepedonium chrysospermum. the unique nature of chrysaibol was recognized by bioactivity-g ... | 2008 | 18702471 |
| basidiomycetous yeasts from boletales fruiting bodies and their interactions with the mycoparasite sepedonium chrysospermum and the host fungus paxillus. | interactions between mushrooms, yeasts, and parasitic fungi are probably common in nature, but are rarely described. bolete fruiting bodies are associated with a broad spectrum of microorganisms including yeasts, and they are commonly infected with filamentous mycoparasites of the genus sepedonium (teleomorph hypomyces). we report the isolation of 17 yeast strains from paxillus and xerocomus, 16 of which were obtained from the surface tissue, the primary site of sepedonium infection. phylogeneti ... | 2011 | 21833540 |
| a hemolytic peptide from the mycophilic fungus sepedonium chrysospermum (bull.) fr. | the hemolytic activity of an extract of the mycoparasite sepedonium chrysospermum (teleomorph hypomyces chrysospermus) was detected and characterized. extraction of the fungal biomass by methanol yielded a fraction in which the hemolytic activity against human red blood cells corresponded to a peptide with a molecular mass of 7,653.72 da and an isoelectric point of approximately 5.8. the peptide was temperature resistant, and the hemolysis was only partially inhibited, even after a 30-min pre-in ... | 2011 | 22080344 |