| kinetics and stability of glucooxidase from penicillium vitale. | the kinetics, thermal stability and ultrasonic resistance of glucosoxidase from penicillium vitale have been investigated. its oxidative constant is close to, catalytic constant is 1.7 times less and the reductive constant is 5.4 times more than the respective constants of the enzyme from aspergillus niger. the relationship between the thermal inactivation constant (k(i)=2.1 pt 15.3. the same relationship is true of the ultrasonic inactivation constant and ph in the ph range of 6.5-8.0. in a mor ... | 1978 | 27781 |
| penicillopepsin from penicillium janthinellum crystal structure at 2.8 a and sequence homology with porcine pepsin. | the polypeptide chain of the acid protease penicillo pepsin folds via an 18-stranded mixed beta-sheet into two distinct lobes separated by a 30-a long groove which is the extended substrate binding site. the catalytic residues asp-32 and asp-215 are located in this groove and their carboxyl groups are in intimate contact. alignment of the amino acid sequence with that of pepsin shows regions of high homology. | 1977 | 323722 |
| penicillopepsin: 2.8 a structure, active site conformation and mechanistic implications. | the crystal structure of penicillopepsin, an extracellular acid protease isolated from the mold penicillium janthinellum, has been determined at 2.8 a resolution by the method of multiple isomorphous replacement. the resulting electron density map computed from the native structure factor amplitudes and mir phases has an overall mean figure of merit of 0.90. the molecule is decidedly nonspherical, with the majority of residues in beta-structure. there is an 18-stranded mixed beta-sheet which for ... | 1977 | 339694 |
| the primary structure of calf chymosin. | the complete amino acid sequence of calf chymosin (rennin) (ec 3.4.23.4) has been determined. the sequence consists of a single peptide chain of 323 amino acid residues. the primary structure of the precursor part of calf prochymosin was published previously (pedersen, v.b., and foltmann, b. (1975) eur. j. biochem. 55, 95-103), thus we are now able to account for the total 365 amino acid residues of calf prochymosin. comparison of the sequence of calf prochymosin with that of pig pepsinogen a (e ... | 1979 | 381305 |
| production of tremorgenic toxins by penicillium janthinellum biourge: a possible aetiological factor in ryegrass staggers. | topsoil, herbage and faeces collected during an outbreak of ryegrass staggers in sheep were examined for tremorgenic penicillia. no such fungi were recovered from the plant material, but they were found among the predominant fungi in the soil and faecal samples. the commonest species of penicillium, and almost the only tremorgenic species encountered, was penicillium janthinellum biourge. when fed to sheep, the mycelium of this fungus evoked a number of the clinical signs seen in field cases of ... | 1979 | 475667 |
| the effects of temperature on growth of four high arctic soil fungi in a three-phase system. | the effect of temperature on the growth of chrysosporium pannorum, cylindrocarpon sp., penicillium janthinellum, and phoma herbarum, isolated from tundra soils, was studied. the growth in two systems, glucose-mineral agar plates and sand, moistened with glucose-mineral broth, was compared. all isolates showed an exponential increase in mass (measured as protein increase) in sand and a linear rate of extension on agar. radial increase on agar was shown not to be a good index of growth in sand. tr ... | 1978 | 565246 |
| [effect of amino acids on transformation of glucose oxidase as antigen in tissues of immunized animals]. | the effect of certain amino acids on transformation of glucoseoxidase as an antigen in different tissues of the animals immunized with it showed that the used glucoseoxidase of the fungus penicillium vitale pidopl. et bilaj possesses antigenic properties peculiar to this enzyme isolated from other sources. 1.5 minutes after a single administration of the antigen to nonimmunized rabbits it is determined in them in descending amounts in such an order: blood, lungs, liver, kidneys, lymphatic nodes. ... | 1978 | 567396 |
| [spectral properties of the prosthetic group of penicillium vitale catalase]. | differences in the absorption spectrum of the penicillum vitale catalase in the visible region as compared to the absorption spectrum for catalase of animal origin are established to be due to the prosthetic group of the enzyme. a molecule of p. vitale catalase is determined to contain 0.051 +/- 0.0003% of iron. it corresponds to two iron atoms per enzyme molecule and to a twice as low content of iron as in a molecule of the bovine liver catalase. an assumption is advanced that the p. vitale cat ... | 1978 | 664035 |
| [method of purification of glucose oxidase by means of affinity chromatography on immunoabsorbent]. | the possibility to purify glucose oxidase from penicillium vitale on immunosorbent containing specific antibodies to the enzyme covalently bound with sepharose 4b is studied. the method of affinity chromatography was applied, beside routine methods of fractionating blood serum proteins, to isolate specific antibodies from antiserum of rabbits immunized with glucose oxidase. immobilized on sepharose glucose oxidase was used as biospecific sorbent. specific antibodies to the enzyme were isolated u ... | 1975 | 1203397 |
| kininase and anti-inflammatory activities of acid carboxypeptidase from penicillium janthinellum. | the acid carboxypeptidase from penicillium janthinellum catalyzed the rapid release of arginine, and the slow release of phenylalanine, proline, serine and glycine from the carboxy-terminal of bradykinin at ph 4.15 to 4.8. anti-inflammatory activity of the acid carboxypeptidase seems to suggest that the enzyme hydrolyzed bradykinin in vivo. | 1975 | 1204716 |
| microbial degradation of the thiolcarbamate herbicide, diallate, in soils and by pure cultures of soil microorganisms. | the disappearance of the herbicide, avadex (40% diallate), from five agricultural soils (differing in either ph, carbon content, or nitrogen content), incubated under sterile and non-sterile conditions, was followed for a period of 20 weeks. avadex was rapidly lost from microbiologically active soils, with over 50% of the applied (2.5 ppm) dosage disappearing within four weeks; losses from sterile soils were much slower with recoveries of over 50% after 20 weeks. incubation of soil with avadex t ... | 1976 | 1267481 |
| [dermatomycoses in workers in enterprises of the microbiological industry]. | mycologic examination of 54 patients with clinical manifestations of dermatomycosis, engaged in glucose oxidase and catalase production has found a producer fungus, penicillium vitale in 31 (38%); 3 out of 37 people contacting with producers of glucamylase and pectofetidine appeared to be infected by aspergillus awamori (1.1%) and aspergillus foetidus (1.1%), respectively. the producers were isolated from the skin of 40 (16.44%) out of 243 workers having no clinical signs of dermatomycosis, main ... | 1992 | 1427292 |
| laminarinase from penicillium funiculosum and its role in release of beta-glucosidase. | an extracellular laminarinase (1----3)-beta-glucan glucohydrolase (ec 3.2.1.6) was purified from culture filtrates of penicillium funiculosum. it was homogeneous on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. it had a mr of 14,000 and isoelectric point of ph 4.2. the apparent km value for lamimarinase was 8.3 mg/ml and vmax was 8 mumol/min/mg. the distribution of beta-glucosidase activity in two different species of penicillium showed that p. funicul ... | 1991 | 1904246 |
| differentiation of species and strains among filamentous fungi by dna fingerprinting. | we have analyzed 11 strains and clones, representing five species (penicillium janthinellum, p. citrioviridae, p. chrysogenum, aspergillus niger, trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the dna of phage m13. the oligonucleotide probes (ct)8, (gtg)5 and (gaca)4, as well as m13 dna, are informative probes for fingerprinting in all genera and species tested. the pro ... | 1991 | 1907892 |
| electrophoretic karyotype of cellulolytic penicillium janthinellum strains. | the genome of the cellulolytic fungus penicillium janthinellum biourge was resolved completely by rotating field electrophoresis. the gel pattern revealed 8-10 different chromosomes. on the basis of data for yeast chromosome size standards from schizosaccharomyces pombe and saccharomyces cerevisiae, chromosome sizes in the range from 2.0 to 8 mb were estimated. by southern hybridization with heterologous probes, the chromosomal locations of rdna, the elongation factor ef1, actin and ubiquitin ha ... | 1991 | 1934133 |
| comparative spectral analysis of mammalian, fungal, and bacterial catalases. resonance raman evidence for iron-tyrosinate coordination. | resonance raman spectra are reported for catalases from bovine liver, the ascomycete fungus aspergillus niger, and the bacterium micrococcus luteus. the vibrational frequencies of the oxidation-, spin-, and coordination number-sensitive spectral bands are indicative of high spin pentacoordinate hemes in the resting ferric enzymes of each of these organisms. this result is in accord with the crystal structure of bovine catalase (fita, i., and rossmann, m.g. (1985) j. mol. biol. 185, 21-37). in co ... | 1989 | 2753885 |
| amino acid sequence of endothiapepsin. complete primary structure of the aspartic protease from endothia parasitica. | the amino acid sequence of endothiapepsin, the aspartic protease from endothia parasitica has been determined. the enzyme consists of 330 residues. the sequence determination was performed exclusively at the protein level. the homology of this fungal milk-clotting enzyme with aspartic proteases is demonstrated by alignment with pepsin, chymosin, gastricsin, renin, and cathepsin d from various vertebrates and proteinase a from saccharomyces cerevisiae showing 25-30% identity. the identity with mu ... | 1987 | 3305016 |
| nucleotide sequence of the saccharomyces cerevisiae ctt1 gene and deduced amino-acid sequence of yeast catalase t. | a 2642-base-pair dna fragment containing the catalase t (ctt1) structural gene of the yeast saccharomyces cerevisiae and its flanking regions has been sequenced. the gene codes for a protein of 562 amino acids (relative molecular mass 64,449) and appears to contain no intron. the amino acid sequence of catalase t derived from the dna sequence shows 40.7% homology (52.2% including conservative replacements) to that of bovine liver catalase. all amino acids previously postulated to participate dir ... | 1986 | 3536508 |
| comparison of beef liver and penicillium vitale catalases. | the structures of penicillium vitale and beef liver catalase have been determined to atomic resolution. both catalases are tetrameric proteins with deeply buried heme groups. the amino acid sequence of beef liver catalase is known and contains (at least) 506 amino acid residues. although the sequence of p. vitale catalase has not yet been determined chemically, 670 residues have been built into the 2 a resolution electron density map and have been given tentative assignments. a large portion of ... | 1986 | 3712444 |
| the nadph binding site on beef liver catalase. | beef liver and human erythrocyte catalases (ec 1.11.1.6) bind nadp tenaciously [kirkman, h. n. & gaetani, g. f. (1984) proc. natl. acad. sci. usa 81, 4343-4348]. the position of nadp on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. in contrast to nearly all other known structures of protein-bound nadp, nad, and fad, the nadp molecule of beef l ... | 1985 | 3856839 |
| autoradiographic method to screen for soil microorganisms which accumulate zinc. | an autoradiographic method was developed to screen for and isolate soil microorganisms which accumulate zinc (zn). diluted soil samples (rubicon fine sand, entic haplorthods [ph 5.9]) were plated on soil extract-glucose agar containing radioactive 65zn. after 7 days of incubation, individual colonies which accumulated sufficient 65zn could be detected by autoradiography. these colonies were isolated and confirmed as zn accumulators in pure culture by using the autoradiographic plate technique. m ... | 1985 | 3883897 |
| action of serine carboxypeptidases on endopeptidase substrates, peptide-4-methyl-coumaryl-7-amides. | carboxypeptidase y hydrolyzed n-substituted peptide-4-methylcoumarin-7-amides (peptide-nh-mec) at ph 7 by releasing 7-amino-4-methylcoumarin (nh2-mec) which was then followed by carboxypeptidase action. in particular, a chymotrypsin-directed substrate, suc-leu-leu-val-tyr-nh-mec, was hydrolyzed by the enzyme with a second-order rate constant of 7200 m-1 s-1, which is compatible with the rate for an anilide substrate and some n-substituted dipeptides. the activity was completely inhibited by phen ... | 1985 | 3905405 |
| the active center of catalase. | the refined structure of beef liver catalase (i. fita, a. m. silva, m. r. n. murthy & m. g. rossmann, unpublished results) is here examined with regard to possible catalytic mechanisms. the distal side of the deeply buried heme pocket is connected with the surface of the molecule by one (or possibly two) channel. the electron density representing the heme group, in each of the two crystallographically independent subunits, is consistent with degradation of the porphyrin rings. the heme group app ... | 1985 | 4046038 |
| submerged production, purification, and crystallization of acid carboxypeptidase from penicillium janthinellum ifo-8070. | penicillium janthinellum ifo-8070 produced an acid carboxypeptidase of molecular weight 51,000 in a liquid medium at 25 c. maximum enzyme concentration was obtained within 3 to 6 days in a medium containing 2% wheat bran, 1% defatted soybean, and 1% kh(2)po(4); the initial ph was 2 to 4. when submerged aerobic conditions were used, a 51,000-molecular-weight acid carboxypeptidase was produced and no detectable amounts of 160,000-molecular-weight acid carboxypeptidase were produced. acid carboxype ... | 1974 | 4474829 |
| [formation of glucose oxidase and catalase during the growth of penicillium vitale pidopl. et bilai on media with different nitrogen and carbon ratios]. | | 1972 | 4667024 |
| the janthitrems: fluorescent tremorgenic toxins produced by penicillium janthinellum isolates from ryegrass pastures. | new tremorgenic mycotoxins named janthitrem a, b, and c (molecular weights 601, 585, and 569, respectively) were produced by more than half of 21 penicillium janthinellum isolates obtained from ryegrass pastures involved in ryegrass staggers outbreaks in sheep. | 1980 | 7356319 |
| the oxidation of pyrene and benzo[a]pyrene by nonbasidiomycete soil fungi. | the purpose of this study was to determine the ability of nonbasidiomycete soil fungi to oxidize pyrene (four rings) and benzo[a]pyrene (bap) (five rings). fungi were isolated from five different soils in which the polycyclic aromatic hydrocarbon content ranged from 0.8 to 80 micrograms/g dry soil. approximately 50% of the isolates in all sites were able to oxidize pyrene. the pyrene-oxidizing species belonged to all fungal divisions except basidiomycetes. the most common were penicillium spp. o ... | 1995 | 7627908 |
| crystal structure of catalase hpii from escherichia coli. | catalase is a ubiquitous enzyme present in both the prokaryotic and eukaryotic cells of aerobic organisms. it serves, in part, to protect the cell from the toxic effects of small peroxides. escherichia coli produces two catalases, hpi and hpii, that are quite distinct from other catalases in physical structure and catalytic properties. hpii, studied in this work, is encoded by the kate gene, and has been characterized as an oligomeric, monofunctional catalase containing one cis-heme d prosthetic ... | 1995 | 7663946 |
| the primary structure of carboxypeptidase s3 from penicillium janthinellum ibt 3991. | the complete amino acid sequence of penicillopeptidase s3, a serine carboxypeptidase isolated from penicillium janthinellum ibt 3991, has been determined. the enzyme consists of 481 amino acids arranged in a single polypeptide chain. six glycosylation sites were established in positions 41, 218, 256, 326, 384 and 392. the molecule contains six cysteinyl residues among which disulfide bridges was established between cys-71-cys-333 and cys-233-cys-289. carboxypeptidase s3 is homologous to carboxyp ... | 1995 | 7664873 |
| production of extracellular xylanases by penicillium janthinellum. effect of selected growth conditions. | xylanase production by penicillium janthinellum using 10-100 mm of 2,2-dimethylsuccinate (dms) buffer, in a range of ph 4.5-6.0 was studied. the enzyme activity was enhanced using oat xylan as the carbon source. under these conditions a culture produced 1.14 mumol/min (11.4 u/ml or 84.4 u/mg) of beta-xylanase after 5 d of growth in a 10-mm buffer solution at ph 4.5. protease was absent in the dms buffer except when 100 mm phosphate buffer at ph 6.0 was used (4 u/ml). beta-xylosidase was only fou ... | 1994 | 7944349 |
| pea (pisum sativum l.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product. | the complete amino acid sequences of the alpha-subunits of pea (pisum sativum l.) seed and root lectin, the c-terminal amino acids of the beta-subunits of pea seed lectin, and most of the sequence of the beta-subunit of pea root lectin were determined. in contrast to earlier reports it was shown that the beta-subunits of both seed isolectins end at asn-181. the alpha 1 subunits end at gln-241 (major fraction) or lys-240 (minor fraction), whereas the alpha 2 subunits end at ser-239, ser-238, ser- ... | 1994 | 8111028 |
| cloning, sequencing, and heterologous expression of a cellulase-encoding cdna (cbh1) from penicillium janthinellum. | from a penicillium janthinellum cdna library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a trichoderma reesei cellulase-encoding gene probe (egl1). both cdnas have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. in the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase i of t. reesei and 70% homology with cellobiohydrolase i ... | 1993 | 8440481 |
| molecular analysis and overexpression of the gene encoding endothiapepsin, an aspartic protease from cryphonectria parasitica. | the gene, epn-1, encoding endothiapepsin (epn), an aspartic protease (aspp) synthesized and secreted by the ascomycete fungus responsible for chestnut blight, cryphonectria (endothia) parasitica, was identified and characterized. inspection of the nucleotide and deduced amino acid (aa) sequences revealed perfect agreement with the experimentally derived 330-aa sequence of mature epn [barkholt, eur. j. biochem. 167 (1987) 327-338] and an additional 89 aa of putative preprosequence. of the nine fu ... | 1993 | 8462868 |
| distribution and properties of fructosyl amino acid oxidase in fungi. | fructosyl amino acid oxidase, and enzyme that can be used for the determination of glycated proteins in blood samples from diabetic patients, was used to screen cultures in our microorganism culture collection. fructosyl amino acid oxidase was found only in the strains of four genera of fungi, aspergillus, fusarium, gibberella, and penicillium and exhibited different substrate specificities against fructosyl valine and n epsilon-fructosyl n alpha-z-lysine. a fructosyl valine-specific enzyme from ... | 1995 | 8534116 |
| structure of the heme d of penicillium vitale and escherichia coli catalases. | a heme d prosthetic group with the configuration of a cis-hydroxychlorin gamma-spirolactone has been found in the crystal structures of penicillium vitale catalase and escherichia coli catalase hydroperoxidase ii (hpii). the absolute stereochemistry of the two heme d chiral carbon atoms has been shown to be identical. for both catalases the heme d is rotated 180 degrees about the axis defined by the alpha-gamma-meso carbon atoms, with respect to the orientation found for heme b in beef liver cat ... | 1996 | 8621527 |
| endoglucanase ii (egii) of penicillium janthinellum: cdna sequence, heterologous expression and promotor analysis. | the cdna coding for the endoglucanase egii of p. janthinellum was cloned and sequenced. the open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the t. reesei egl2. the cellulose-binding domain of egii represents a typical member of the a family of cellulases. the egl2 gene is only induced by cellulose or cellobiose and not by sophorose. a promotor fragment including 1 kb was cloned and sequenced. three major transcription startp ... | 1996 | 8625430 |
| primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins. | fructosyl amino acid oxidase (faod), which is active toward model compounds of the glycated proteins in blood, n epsilon-fructosyl n sigma-z-lysine and n-fructosyl valine, was purified to homogeneity from aspergillus terreus gp1. though the enzyme did not use glycated proteins directly as its substrate, it used glycated human serum albumin (hsa) when hsa was treated with a protease. linear relationships between both the concentration and the increase in absorbance and the glycation rate of glyca ... | 1996 | 9022674 |
| [complete amino acid sequence of catalase from the fungus penicillium vitale]. | the polypeptide sequence of the unmodified catalase from penicillium vitale containing 696 amino acid residues was deduced. the sequences of 76 tryptic peptides of the unmodified catalase, 63 tryptic peptides of the catalase with modified lys residues, 48 peptides resulting from catalase cleavage by the staphylococcus aureus v8 protease, and 9 fragments obtained by brcn-treatment were considered, and a comparison with the sequences of other catalases was made. | 1998 | 9612556 |
| the amino acid sequences of carboxypeptidases i and ii from aspergillus niger and their stability in the presence of divalent cations. | the amino acid sequences of serine carboxypeptidase i (cpd-i) and ii (cpd-ii), respectively, from aspergillus niger have been determined by conventional edman degradation of the reduced and vinylpyridinated enzymes and peptides hereof generated by cleavage with cyanogen bromide, iodobenzoic acid, glutamic acid cleaving enzyme, aspn-endoproteinase and endolysc proteinase. cpd-i consists of a single peptide chain of 471 amino acid residues, three disulfide bridges and nine n-glycosylated asparagin ... | 1998 | 9748653 |
| degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures. | this study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (pahs) in liquid media and soil by bacteria (stenotrophomonas maltophilia vun 10,010 and bacterial consortium vun 10,009) and a fungus (penicillium janthinellum vuo 10, 201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. the bacteria could use pyrene as their sole carbon and energy source in a basal salts medium (bsm) and mineralized significant amounts ... | 2000 | 10698765 |
| penicillopepsin-jt2, a recombinant enzyme from penicillium janthinellum and the contribution of a hydrogen bond in subsite s3 to k(cat). | the nucleotide sequence of the gene (pepa) of a zymogen of an aspartic proteinase from penicillium janthinellum with a 71% identity in the deduced amino acid sequence to penicillopepsin (which we propose to call penicillopepsin-jt1) has been determined. the gene consists of 60 codons for a putative leader sequence of 20 amino acid residues, a sequence of about 150 nucleotides that probably codes for an activation peptide and a sequence with two introns that codes for the active aspartic proteina ... | 2000 | 10850809 |
| isolation and characterization of acid- and al-tolerant microorganisms. | acid- and aluminum (al)-tolerant microorganisms were isolated from tea fields, from which six strains were selected and identified as cryptococcus humicola, rhodotorula glutinis, aspergillus flavus link, penicillium sp., penicillium janthinellum biourge and trichoderma asperellum. they were tolerant to al up to 100-200 mm and could grow at low ph, 2.5-2.2. in a glucose medium (ph 3.5) the ph of the spent medium decreased to below 3.0. the toxic inorganic monomeric al in the spent medium decrease ... | 2000 | 10930728 |
| the chitinolytic activity of penicillium janthinellum p9: purification, partial characterization and potential applications. | to purify and characterize the chitinolytic activity of penicillium janthinellum p9 and to evaluate possible uses of the purified enzymes in the control of fungal growth and spore germination. | 2001 | 11556916 |
| degradation of pyrene by indigenous fungi from a former gasworks site. | indigenous fungi isolated from soil of a former gasworks site were investigated in submerged cultures with pyrene as the sole carbon source. five fungal strains capable of degrading pyrene included one strain of trichoderma harzianum and four strains with characteristics of the genus penicillium. these are identified as penicillium simplicissimum, penicillium janthinellum, penicillium funiculosum and penicillium terrestre. a maximum of 75% of 50 mg l(-1) and 67% of 100 mg l(-1) of pyrene was rem ... | 2002 | 12044679 |
| the extremely high al resistance of penicillium janthineleum f-13 is not caused by internal or external sequestration of al. | penicillium janthinellum f-13 has been isolated in previous work as a fungus tolerating the presence of high concentrations of al (as high as 100 mm alcl3). here its growth rate and yield in three acidic (ph 3.0) media of different composition with varying concentrations of al are reported. the presence of al did not affect these parameters. except that the growth yield was somewhat lower in gm (a glucose/peptone/yeast extract-containing medium) with the highest concentration tested (100 mm alcl ... | 2002 | 12046925 |
| effect of salinity and temperature on coccidioides immitis and three antagonistic soil saprophytes. | egeberg, roger o. (university of southern california, los angeles), ann f. elconin, and margaret c. egeberg. effect of salinity and temperature on coccidioides immitis and three antagonistic soil saprophytes. j. bacteriol. 88:473-476. 1964.-experiments exploring the factors which determine the ability of coccidioides immitis to survive and reproduce in the soil in situ are described and discussed. previous work indicated that soil temperatures and salinity influence the growth and distribution o ... | 1964 | 14203366 |
| liquid chromatography-tandem mass spectrometry for the identification of l-tetrahydropalmatine metabolites in penicillium janthinellum and rats. | l-tetrahydropalmatine (l-thp) is an active alkaloid from stephania ainiaca diels. in order to compare the similarities and differences of microbial and mammalian metabolisms of l-thp, the microbial transformation by penicillium janthinellum and metabolism in rats were investigated. biotransformation of l-thp by penicillium janthinellum as 3.510 resulted in the formation of three metabolites. their structures were identified as l-corydalmine, l-corypalmine and 9-o-desmethyl-l-thp, respectively, b ... | 2006 | 15959869 |
| subcellular localization of fructosyl amino acid oxidases in peroxisomes of aspergillus terreus and penicillium janthinellum. | fructosyl amino acid oxidase (faod) is the enzyme catalyzing the oxidative deglycation of amadori compounds, such as fructosyl amino acids, yielding the corresponding amino acids, glucosone, and h(2)o(2). in a previous report, we determined the primary structures of cdnas coding for faods from two fungal strains aspergillus terreus ap1 and penicillium janthinellum and we found that both fungal faods included the putative peroxisome targeting signal 1 (pts1) at the carboxyl terminal (yoshida, n. ... | 1999 | 16232435 |
| studies on the interaction between grape-associated filamentous fungi on a synthetic medium. | eleven fungi isolated from grapes and sun-dried grapes (alternaria alternata, cladosporium herbarum, eurotium amstelodami, penicillium janthinellum, p. decumbens, trichoderma harzianum, candida sp., aspergillus carbonarius ota-negative, a. carbonarius ota-positive, a. niger var. niger. and a. japonicus var. aculeatus), were grown in snm medium at different water activities (0.82-0.97) and temperature (20-40 degrees c) levels for 18 days. pairs of one aspergillus faced with one non-aspergillus we ... | 2007 | 16996158 |
| [collagenolytic activity in several species of deuteromycetes under various storage conditions]. | the ability of deuteromycetes of the genera penicillium, aspergillus, and botrytis to retain collagenolytic activity was studied after both 2 and 10 years of storage on a czapek medium under a layer of mineral oil at 4 degrees c, as well as in silica gel granules at 20 and -60 degrees c. the enzymatic activity of several species, including botrytis terrestris, penicillium janthinellum, penicillium chrysogenum, and penicillium citrinum, was retained under both conditions of storage. aspergillus r ... | 2006 | 17022462 |
| effect of intra and interspecific interaction on ota production by a. section nigri in grapes during dehydration. | the aim of this study was to assess the colonizing capability and ota production of different populations of aspergillus section nigri spp. in grapes, as affected by the interactions with other fungi, during a simulated in vitro sun-drying. mature white grapes were divided into two lots of healthy and artificially injured grapes and inoculated with a. section nigri spp. (a. carbonarius ota producer, a. niger aggregate ota producer, a. niger aggregate ota non-producer), eurotium amstelodami and p ... | 2007 | 17188203 |
| the structures and electronic configuration of compound i intermediates of helicobacter pylori and penicillium vitale catalases determined by x-ray crystallography and qm/mm density functional theory calculations. | the structures of helicobacter pylori (hpc) and penicillium vitale (pvc) catalases, each with two subunits in the crystal asymmetric unit, oxidized with peroxoacetic acid are reported at 1.8 and 1.7 a resolution, respectively. despite the similar oxidation conditions employed, the iron-oxygen coordination length is 1.72 a for pvc, close to what is expected for a fe=o double bond, and 1.80 and 1.85 a for hpc, suggestive of a fe-o single bond. the structure and electronic configuration of the oxof ... | 2007 | 17358056 |
| indole alkaloids produced by a marine fungus isolate of penicillium janthinellum biourge. | three new indole alkaloids, shearinines d, e, and f (1-3), together with the known shearinine a (4) were isolated from the marine-derived strain of the fungus penicillium janthinellum biourge. the chemical structures of 1-4 were established by 2d nmr and hreims data. shearinines a, d, and e induce apoptosis in human leukemia hl-60 cells, and shearinine e also inhibits egf-induced malignant transformation of jb6 p+ cl 41 cells in a soft agar. | 2007 | 17555349 |
| effect of preharvest fungicides and interacting fungi on aspergillus carbonarius growth and ochratoxin a synthesis in dehydrating grapes. | to evaluate the effect of preharvest grape pesticides in aspergillus section nigri infection in dehydrating grapes and the final ochratoxin a (ota) content. additionally, the effect of coinoculation of moulds frequently isolated from grapes and raisins on aspergillus section nigri infection was studied. | 2007 | 17651218 |
| effect of germicidal uvc light on fungi isolated from grapes and raisins. | to examine how uvc affects the different genera of fungi commonly isolated from grapes, with the aim of understanding changes in mycobiota during grape ripening and possible applications for preventing grape decay during storage. | 2007 | 17718833 |
| a new indoloditerpene derivative, penijanthine a, isolated from penicillium janthinellum. | in a screen searching for new bioactive agents, a new indoloditerpene, penijanthine a (1), was isolated from penicillium janthinellum ifm 55557. the structure of 1 was established on the basis of spectroscopic and chemical investigation, as well as detailed comparison with the spectroscopic and physico-chemical data of paxilline (2), which was isolated along with 1. | 2009 | 18850072 |
| the molecular mechanism of the catalase reaction. | catalases are ubiquitous enzymes that prevent cell oxidative damage by degrading hydrogen peroxide to water and oxygen (2h(2)o(2) --> 2 h(2)o + o(2)) with high efficiency. the enzyme is first oxidized to a high-valent iron intermediate, known as compound i (cpd i) which, in contrast to other hydroperoxidases, is reduced back to the resting state by further reacting with h(2)o(2). by means of hybrid qm/mm car-parrinello metadynamics simulations, we have investigated the mechanism of the reduction ... | 2009 | 19653683 |
| functional characterization of the copper-transporting p-type atpase gene of penicillium janthinellum strain gxcr. | copper (cu)-transporting p-type atpase (ctpa) genes have been documented to play an important role in resistance to heavy metals. however, our understanding of roles of ctpa genes of the filamentous fungi was based only on sequence similarity prediction before. in a previous study, we isolated a penicillum janthinellum strain gxcr of higher tolerance to cu (200 mm). in this study, we cloned the partial cdna of ctpa gene, named pcpa, from the strain gxcr. sequence alignment indicated that the clo ... | 2010 | 20127468 |
| culture-independent characterization of bacteria and fungi in a poultry bioaerosol using pyrosequencing: a new approach. | work in animal production facilities often results in exposure to organic dusts. previous studies have documented decreases in pulmonary function and lung inflammation among workers exposed to organic dust in the poultry industry. bacteria and fungi have been reported as components of the organic dust produced in poultry facilities. to date, little is known about the diversity and concentration of bacteria and fungi inside poultry buildings. all previous investigations have utilized culture-base ... | 2010 | 21058154 |
| screening filamentous tropical fungi for their nutritional potential as sources of crude proteins, lipids and minerals. | the use of fungal and yeast biomass in foodstuff, either as supplements or as major ingredients in formulations is an area of growing interest for the modern food industry. the aim of this study was to evaluate the nutritional potential of biomasses obtained from filamentous fungi penicillium sclerotiorum, penicillium janthinellum, rhizopus stolonifer and syncephalastrum racemosum. biomasses presented 26-37% of total proteins, 1.7-3.5% of lipids and 4.6-9.1% of ashes. the humidity level reached ... | 2010 | 21339148 |
| proton transfer drives protein radical formation in helicobacter pylori catalase but not in penicillium vitale catalase. | heme catalases prevent cells from oxidative damage by decomposing hydrogen peroxide into water and molecular oxygen. here we investigate the factors that give rise to an undesirable side reaction competing with normal catalase activity, the migration of a radical from the heme active site to the protein in the principal reaction intermediate compound i (cpd i). recently, it has been proposed that this electron transfer reaction takes place in cpd i of helicobacter pylori catalase (hpc), but not ... | 2011 | 21381757 |
| Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1. | Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l(-1) of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation ... | 2011 | 21920586 |
| thirty years of heme catalases structural biology. | about thirty years ago the crystal structures of the heme catalases from penicillium vitale (pvc) and, a few months later, from bovine liver (blc) were published. both enzymes were compact tetrameric molecules with subunits that, despite their size differences and the large phylogenetic separation between the two organisms, presented a striking structural similarity for about 460 residues. the high conservation, confirmed in all the subsequent structures determined, suggested a strong pressure t ... | 2011 | 22209752 |
| [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut]. | We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping. | 2011 | 21866710 |
| biotransformation of imperatorin by penicillium janthinellum. anti-osteoporosis activities of its metabolites. | imperatorin (imp) is a major constituent of many herbal medicines and possesses anti-osteoporosis activity. the present research work aimed to study the biotransformation processes of imp and evaluated the anti-osteoporosis activity of the transformed metabolites. among 18 strains of filamentous fungi screened, penicillium janthinellum as 3.510 exhibited good capability to metabolise imp to the new derivatives. ten transformed products were isolated and purified, and their structures were identi ... | 2012 | 23497884 |
| isolation, identification and antimicrobial activity of propolis-associated fungi. | propolis is a natural product widely known for its medicinal properties. in this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. twenty-two fungal isolates were obtained, some of which were identified as alternaria alternata, aspergillus flavus, bipolaris hawaiiensis, fusarium merismoides, lasiodiplodia theobromae, penicillium citrinum, penicillium crustosum, penicillium janthinellum, penicillium purpurogenum, pestalot ... | 2013 | 23439233 |
| antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina. | breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from. | 2013 | 23727185 |
| [characteristics of the eukaryotic community structure in acid mine drainage lake in anhui province, china]. | we characterized eukaryotic community structure and the relationship between the community structure and environmental factors in acidic mine drainage (amd) lake of a sulfide mine in anhui province, china. | 2012 | 23115972 |
| structure, recombinant expression and mutagenesis studies of the catalase with oxidase activity from scytalidium thermophilum. | scytalidium thermophilum produces a catalase with phenol oxidase activity (catpo) that catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds. a codon-optimized catpo gene was cloned and expressed in escherichia coli. the crystal structures of native and recombinant s. thermophilum catpo and two variants, h82n and v123f, were determined at resolutions of 2.7, 1.4, 1.5 and 1.9 å, respectively. the structure of catpo reveals a homotetram ... | 2013 | 23519415 |
| non-hydrolytic disruption of crystalline structure of cellulose by cellulose binding domain and linker sequence of cellobiohydrolase i from penicillium janthinellum. | the cooperation between cellobiohydrolase (cbhi) and endoglucanase (eg) is necessary for biodegradation of native cellulose, but its mechanism is still poorly understood. the present paper report at the first time that an isolated component, the cellulose binding domain with its linker sequence of cellobiohydrolase i from penicillium janthinellum (cbd(cbhi)), plays an important role in the synergism between cbhi and egi during cellulose biodegradation. a recombinantplasmid (puc18c), containing t ... | 2001 | 12053182 |
| concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome. | microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ... | 2017 | 28336093 |
| demethylation of arsenic limits its volatilization in fungi. | arsenic (as) biomethylation is increasingly being regarded as a promising method to volatize as from the environment; however, the as volatilization efficiency of most microorganisms is low. here, the speciation transformation of dimethylarsinic acid (dma) as an important methylation intermediate in the cells of fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1 were investigated. these fungal strains have been certified to volatilize as from as-loade ... | 2015 | 25951513 |
| arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using hplc-hg-afs and in-situ xanes. | microorganisms dominated speciation of arsenic (as) play an important role in the biogeochemical cycling of as. in the study, species transformation of arsenite [as(iii)] and as(iii) influx and efflux across the cell membranes of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 cells were studied using a cellular lysis plus chromatographic separation method and further the in-situ x-ray absorption near edge structure (xanes) analysis. the results in ... | 2015 | 25460757 |
| arsenate reduction and methylation in the cells of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 investigated with x-ray absorption near edge structure. | synchrotron radiation-based x-ray absorption near edge structure (xanes) was introduced to directly analysis chemical species of arsenic (as) in the cells of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 capable of as accumulation and volatilisation. after exposure to as(v) of 500 mg l(-1) for 15 days, a total of 60.5% and 65.3% of the accumulated as in the cells of t. asperellum sm-12f1 and p. janthinellum sm-12f4, respectively, was as(iii), fol ... | 2012 | 23122191 |
| penicilones a-d, anti-mrsa azaphilones from the marine-derived fungus penicillium janthinellum hk1-6. | four new azaphilones, penicilones a-d (1-4), were isolated from the mangrove rhizosphere soil-derived fungus penicillium janthinellum hk1-6. their planar structures and absolute configurations were determined by extensive analysis of nmr spectroscopic data, ecd spectra, the modified mosher's method, and chemical conversions. interestingly, 1 and 2 had the opposite configuration at c-7 compared to the closely related chloro analogues 3 and 4. ester hydrolysis of 2 and 4 afforded their parental az ... | 2017 | 28248508 |
| chemical characterization of fatty acids, alkanes, n-diols and alkyl esters produced by a mixed culture of trichoderma koningii and penicillium janthinellum grown aerobically on undecanoic acid, potatoe dextrose and their mixture. | little is known about the mixed fungal synthesis of high-value aliphatics derived from the metabolism of simple and complex carbon substrates. trichoderma koningii and penicillium janthinellum were fed with undecanoic acid (uda), potatoe dextrose broth (pdb), and their mixture. pyrolysis field ionization mass spectrometry (py-fims) together with (1)h and (13)c nuclear magnetic resonance (nmr) characterized chcl3 soluble aliphatics in the fungal cell culture. data from nmr and py-fims analysis we ... | 2016 | 26852878 |
| metabolism of nc11 fatty acid fed to trichoderma koningii and penicillium janthinellum ii: production of intracellular and extracellular lipids. | little is known about the fungal metabolism of nc10 and nc11 fatty acids and their conversion into lipids. a mixed batch culture of soil fungi, t. koningii and p. janthinellum, was grown on undecanoic acid (uda), a mixture of uda and potato dextrose broth (uda+pdb), and pdb alone to examine their metabolic conversion during growth. we quantified seven intracellular and extracellular lipid classes using iatroscan thin-layer chromatography with flame ionization detection (tlc-fid). gas chromatogra ... | 2014 | 25310811 |
| metabolism of n-c10:0 and n-c11:0 fatty acids by trichoderma koningii, penicillium janthinellum and their mixed culture: i. biomass and co2 production, and allocation of intracellular lipids. | the capacity of two soil fungi, trichoderma koningii and penicillium janthinellum, to oxidize n-c10:0 and n-c11:0 fatty acids to co2 and store intracellular lipids during growth is unknown. this article reports for the first time the metabolism of decanoic acid (da, c10:0), undecanoic acid (uda, n-c11:0), a mixture of the acids (uda+da) and a mixture of uda+ potato dextrose broth (pdb) by t. koningii and p. janthinellum and their mixed culture. a control pdb complex substrate was used as a subst ... | 2014 | 25310810 |
| fungal communities in the garden chamber soils of leaf-cutting ants. | leaf-cutting ants modify the properties of the soil adjacent to their nests. here, we examined whether such an ant-altered environment impacts the belowground fungal communities. fungal diversity and community structure of soil from the fungus garden chambers of atta sexdens rubropilosa and atta bisphaerica, two widespread leaf-cutting ants in brazil, were determined and compared with non-nest soils. culture-dependent methods revealed similar species richness but different community compositions ... | 2014 | 23681670 |
| janthinolide a-b, two new 2,5-piperazinedione derivatives from the endophytic penicillium janthinellum isolated from the soft coral dendronephthya sp. | two new 2,5-piperazinedione derivatives, janthinolide a and b (1-2), along with deoxymycelianamide, griseofulvin and dechlorogriseofulvin were isolated from the fermentation broths of the endophytic fungus penicillium janthinellum isolated from a soft coral, dendronephthya sp. their structures were established by extensive spectroscopic data analysis. | 2006 | 17283665 |
| production of xylanase and protease by penicillium janthinellum crc 87m-115 from different agricultural wastes. | five agricultural wastes were evaluated in submerged fermentation for xylanolytic enzymes production by penicillium janthinellum. the wastes were hydrolyzed in acid medium and the liquid fraction was used for cultivation. corn cob (55.3 u/ml) and oat husk (54.8 u/ml) were the best inducers of xylanase. sugar cane bagasse (23.0 u/ml) and corn husk (23.8 u/ml) were moderately good, while cassava peel was negligible. protease production was very low in all agro-industrial residues. the maximum biom ... | 2006 | 15953719 |
| pyrene is metabolized to bound residues by penicillium janthinellum sfu403. | we have previously shown that the filamentous fungus, penicillium janthinellum sfu403 (sfu403) oxidizes pyrene to pyrene 1,6- and 1,8-quinones and that the level of pyrenequinones (pqs) subsequently declines suggesting that pqs are not terminal metabolites. the purpose of this study was to determine the fate of pqs in sfu403. first, we compared the fate of 14c-pyrene in sfu403 and a non-pyrene-oxidizing fungus, a paecilomyces sp.. after 7 days of incubation, more than 80% of the radioactivity wa ... | 2000 | 11487060 |
| antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi. | medicinal plants are used by many ethnic groups as a source of medicine for the treatment of various ailments in both humans and domestic animals. these plants produce secondary metabolites that have antimicrobial properties, thus screening of medicinal plants provide another alternative for producing chemical fungicides that are relatively non-toxic and cost-effective. | 2016 | 28852739 |
| antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi. | major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of alzheimer's disease have led to searching new control drugs. the present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. for such finality, five filamentous fungal species (t ... | 2017 | 28818332 |
| cytotoxic metabolites from the cultures of endophytic fungi from panax ginseng. | two strains of endophytic fungi, penicillium melinii yuan-25 and penicillium janthinellum yuan-27, with strong anti-pyricularia oryzae activity, were obtained from the roots of panax ginseng. based on bioactivity-oriented isolation, a new benzaldehyde derivative, ginsenocin (1), together with six known compounds, methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate (2), 3,4,5-trimethyl-1,2-benzenediol (3), penicillic acid (4), mannitol (5), ergosterol (6), and ergosterol peroxide (7), were separated fro ... | 2013 | 23793342 |
| penicillium araracuarense sp. nov., penicillium elleniae sp. nov., penicillium penarojense sp. nov., penicillium vanderhammenii sp. nov. and penicillium wotroi sp. nov., isolated from leaf litter. | several species of the genus penicillium were isolated during a survey of the mycobiota of leaf litter and soil in colombian amazon forest. five species, penicillium penarojense sp. nov. (type strain cbs 113178(t) = ibt 23262(t)), penicillium wotroi sp. nov. (type strain cbs 118171(t) = ibt 23253(t)), penicillium araracuarense sp. nov. (type strain cbs 113149(t) = ibt 23247(t)), penicillium elleniae sp. nov. (type strain cbs 118135(t) = ibt 23229(t)) and penicillium vanderhammenii sp. nov. (type ... | 2011 | 20639229 |
| isolation of a fungus pencicillium sp. with zinc tolerance and its mechanism of resistance. | a zinc (zn)-tolerant fungus, designated bc109-2, was isolated from rhizosphere soil and was identified as penicillium janthinellum bc109-2 based on its sequence analysis. to understand its zn tolerance mechanisms, a series of studies was carried out addressing the subcellular distribution of zn, its chemical forms, and the antioxidant system (superoxide dismutase, catalase, peroxidase, glutathione reductase, glutathione s-transferase, reduced glutathione, oxidized glutathione and malondialdehyde ... | 2017 | 28887685 |
| a proteomic-based investigation of potential copper-responsive biomarkers: proteins, conceptual networks, and metabolic pathways featuring penicillium janthinellum from a heavy metal-polluted ecological niche. | filamentous fungi-copper (cu) interactions are very important in the formation of natural ecosystems and the bioremediation of heavy metal pollution. however, important issues at the proteome level remain unclear. we compared six proteomes from cu-resistant wild-type (wt) penicillium janthinellum strain gxcr and a cu-sensitive mutant (ec-6) under 0, 0.5, and 3 mmol/l cu treatments using itraq. a total of 495 known proteins were identified, and the following conclusions were drawn from the result ... | 2017 | 28488414 |
| penicisulfuranols a-f, alkaloids from the mangrove endophytic fungus penicillium janthinellum hdn13-309. | six new epipolythiodioxopiperazine (etp) alkaloids, penicisulfuranols a-f (1-6), were isolated from the mangrove endophytic fungus penicillium janthinellum hdn13-309. all structures including absolute configurations were elucidated on the basis of comprehensive spectroscopic data and ecd calculations. they belong to the unusual family of etps containing sulfur atoms on both α- and β-positions of amino acid residues and a rare 1,2-oxazadecaline core moiety. in addition, compounds 1-6 also possess ... | 2017 | 27992183 |
| production, purification and characterization of an exo-polygalacturonase from penicillium janthinellum sw09. | a soil isolate, penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-pg). by optimizing growth conditions, p. janthinellum sw09 produced high amount of exo-pg (16.54 units/ml). the crude enzyme was purified by gel filtration chromatography and two exo-pg activity peaks (designated as pgi and pgii) were revealed. on sds-page analysis, purified pgii using deae-sepharose ff column, was foun ... | 2016 | 27142550 |
| untreated wheat straw: potential source for diverse cellulolytic enzyme secretion by penicillium janthinellum ems-uv-8 mutant. | study describes the production of cellulases by penicillium janthinellum ems-uv-8 using untreated wheat straw (ws), treated ws (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-ii and carboxymethyl cellulose). severely pretreated ws and cellulose-ii produced more cellulolytic enzymes than untreated samples. xrd and ftir analysis revels that the increase in the amorphous structure of pretreated ws/cellulose increases enzyme production. enzyme samples pr ... | 2015 | 26291411 |
| biodegradation of kerosene: study of growth optimization and metabolic fate of p. janthinellum sdx7. | penicillum janthinellum sdx7 was isolated from aged petroleum hydrocarbon-affected soil at the site of anand, gujarat, india, and was tested for different ph, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of ph 6.0, 30 °c and 180 rpm agitation. the gc/ms chromatograms revealed that then-alkane fractions are easily degra ... | 2015 | 26273254 |
| paths and determinants for penicillium janthinellum to resist low and high copper. | copper (cu) tolerance was well understood in fungi yeasts but not in filamentous fungi. filamentous fungi are eukaryotes but unlike eukaryotic fungi yeasts, which are a collection of various fungi that are maybe classified into different taxa but all characterized by growth as filamentous hyphae cells and with a complex morphology. the current knowledge of cu resistance of filamentous fungi is still fragmental and therefore needs to be bridged. in this study, we characterized cu resistance of pe ... | 2015 | 26265593 |
| phytohormones enabled endophytic fungal symbiosis improve aluminum phytoextraction in tolerant solanum lycopersicum: an examples of penicillium janthinellum lk5 and comparison with exogenous ga3. | this work investigates the potentials of fungal-endophyte penicillium janthinellum lk5 (pjlk5) and its inherent gibberellic acid (ga3) as reference to enhance aluminum (al) induced toxicity in tolerant tomato (solanum lycopersicum) plants. initial screening showed significantly higher uptake of al by pjlk5. aluminum stress (100 μm) significantly retarted plant growth in control plants. conversely pjlk5 and ga3 application significantly increased morphological attributes of al-tolerant tomato pla ... | 2015 | 25885165 |
| selection and molecular characterization of cellulolytic-xylanolytic fungi from surface soil-biomass mixtures from black belt sites. | plant biomass is an abundant renewable natural resource that can be transformed into chemical feedstocks. enzymes used in saccharification of lignocellulosic biomass are a major part of biofuel production costs. a cocktail of cellulolytic and xylanolytic enzymes are required for optimal saccharification of biomass. accordingly, thirty-two fungal pure cultures were obtained from surface soil-biomass mixtures collected from black belt sites in alabama by culturing on lignocellulosic biomass medium ... | 2015 | 25817459 |
| penicillium janthinellum: a source of efficient and high levels of β-glucosidase. | penicillium janthinellum strain isolated from leaf litters of oak trees from montane alpine forests of shivalik hills (india) produced high levels of β-glucosidase both during solid-state fermentation (796 units/gds) and shake flask cultures (65.3 units/ml). the peptide mass fingerprinting of the secretome showed a variety of glycosyl hydrolases. β-glucosidase was purified and characterized to be a gh3 family member that had a molecular weight (m r) of 101 kda and pi of 4.5. β-glucosidase was op ... | 2015 | 25351628 |
| bioethanol production from wheat straw via enzymatic route employing penicillium janthinellum cellulases. | this study concerns in-house development of cellulases from a mutant penicillium janthinellum ems-uv-8 and its application in separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes for bioethanol production from pre-treated wheat straw. in a 5l fermentor, the above strain could produce cellulases having activity of 3.1 fpu/ml and a specific activity of 0.83 fpu/mg of protein. in-house developed cellulase worked more efficiently in case of ss ... | 2014 | 25086433 |
| production and purification of a fungal chitosanase and chitooligomers from penicillium janthinellum d4 and discovery of the enzyme activators. | chitosanases have received much attention because of their wide range of applications. although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (spp)-containing penicillium janthinellum d4 medium and purified by ammonium sulphate precipitation and combined column chromatography. the purified d4 chitosanase exhibited optimum activity at ph 7-9, 60°c and was stable at ph 7-11, 25-50°c. the d4 chitosanase that w ... | 2014 | 24751281 |
| fungal endophyte penicillium janthinellum lk5 improves growth of aba-deficient tomato under salinity. | an endophytic fungus was isolated from the roots of tomato (solanum lycopersicum mill) and identified as penicillium janthinellum lk5. the culture filtrate (cf) of p. janthinellum significantly increased the shoot length of gibberellins (gas) deficient mutant waito-c and normal dongjin-beyo rice seedlings as compared to control. the cf of p. janthinellum contained gas (ga3, ga4, ga7 and ga12). to assess endophyte-growth promoting and stress-tolerance potential, the cf along with the propagules o ... | 2013 | 23842755 |
| production of intracellular β-xylosidase from the submerged fermentation of citrus wastes by penicillium janthinellum mtcc 10889. | production of intracellular β-xylosidase was studied in cultures of penicillium janthinellum grown on citrus fruit waste supplemented cultivation media. both dried orange peel and sweet lime peel could induce the production of this enzyme. the working strain showed a pronounced optimum ph and temperature for β-xylosidase production at 6.0 and 27 °c, respectively. the enzyme production was found to remain stable for a long period of 120 h. orange peel and sweet lime peel showed different response ... | 2013 | 28324373 |