| activation parameters for the cleavage reaction of cytidine-2',3'-monophosphate catalyzed by penicillium brevicompactum and aspergillus clavatus rnases. | on the basis of coincidence of all activation parameters (e, deltah*, deltaf* and deltas*) for the reaction of cleavage of cytidine-2';3'-monophosphate catalyzed by "acid" (ph-optimum 4.7) nonspecific rnases from aspergillus clavatus and penicillium brevicompactum (ec 3. 1. 4. 23) it has been proposed that the mechanism of action on this reaction stage (hydrolysis) for both enzymes is equal. | 1978 | 23869 |
| microbial transformations of natural antitumor agents. 9. o-demethylation of 9-methoxyellipticine. | microbial transformation studies were conducted with 9-methoxyellipticine (1). several microorganisms including botrytis allii (nrrl 2502), cunninghamella echinulata (nrrl 1386), c. echinulata (nrrl 3655), and penicillium brevicompactum (atcc 10418) achieved o-demethylation of (1) in good yield. p. brevicompactum was used to prepare 9-hydroxyellipticine (4) from 1 for isolation and complete identification. high-performance liquid chromatography was used to verify the identity of the major metabo ... | 1979 | 575547 |
| volatile metabolites produced by six fungal species compared with other indicators of fungal growth on cereal grains. | six fungal species, penicillium brevicompactum, p. glabrum, p. roqueforti, aspergillus flavus, a. versicolor, and a. candidus, were inoculated on moistened and autoclaved wheat and oat grains. they were cultivated in glass vessels provided with an inlet and outlet for air. air was passed through the vessels to collect volatile fungal metabolites on porous polymer adsorbents attached to the outlet. samples were collected at two fungal growth stages. adsorbed compounds were thermally desorbed, sep ... | 1992 | 1514807 |
| keratinolytic and keratinophilic fungi in the soils of papua new guinea. | a study was made of soil samples collected during an expedition to the highlands of papua new guinea. fungi were isolated from the samples by the method of hair baiting (to-ka-va). of the 33 species isolated, about half showed keratinolytic activity. such activity is previously unreported for mucor hiemalis f. hiemalis, myrothecium roridum, paecilomyces carneus, p. marquandii, penicillium brevicompactum, rhinocladiella mansonii and verticillium lecanii. the species most active keratinolytically ... | 1991 | 1723786 |
| serum igg antibody concentrations against environmental microbes in mares and foals during different seasons and effect of stabling practices. | over periods of 22 and 14 months, igg antibody concentrations in serum samples obtained monthly from 14 mares and 19 foals, respectively, were measured by use of elisa against antigens of the following environmental microbes: aspergillus umbrosus, penicillium brevicompactum, rhodotorula glutinis, absidia corymbifera, aspergillus fumigatus, humicola grisea, micropolyspora faeni, and thermoactinomyces vulgaris. the mares and foals were on pasture from early june until early october, then were stab ... | 1990 | 2327613 |
| [analysis of the guanyl-specific ribonuclease structures in fungi: determination of the amino acid sequence and prediction of the secondary ribonuclease structure in penicillium brevicompactum]. | | 1985 | 3922722 |
| [extracellular rnaase of penicillium brevicompactum]. | | 1971 | 4332106 |
| [phase i study of a new anticancer agent cam--results of cooperative study]. | cam is a derivative compound of mycophenolic acid produced by penicillium brevicompactum, and is a new oral purine antagonistic anticancer agent. the phase i study was carried out cooperatively in ten hospitals. the results are as follows: the administration method was single administration and the starting dose was 200 mg/m2 (1n). the dose level was escalated according to varied fibonacci formula. the number of total cases was thirty-one: three cases at 1n level, four at 2n, six at 3.3n, six at ... | 1982 | 7184385 |
| fungal propagules in house dust. ii. relation with residential characteristics and respiratory symptoms. | as part of a case-control study on the relation between home dampness and respiratory symptoms of children, house-dust samples were collected from bedroom floors and mattresses in 60 homes in the netherlands. the house-dust samples were analyzed for the presence of fungal propagules by plating 30 mg of dust directly onto dg18 agar. a checklist and questionnaire were used to obtain information on the home characteristics and occupant behavior that may have an effect on the presence of fungal prop ... | 1994 | 7825721 |
| nmr studies of a complex of rnase from penicillium brevicompactum with dinucleoside phosphonate and the implications for the mechanism of enzyme action. | the chemical-shift dependences of the proton signals of the guanosine and uridine moieties were measured as a function of the relative amount of gpcu complexed with rnase pb1 (ec 3.1.27.3). the equal values of the chemical-shift changes of the guanosine c8-protons on complex formation between gpcu and rnase pb1 and that of the 3'-gmp and rnase pb1 allow to conclude that the guanosine base is bound in the same manner in these protein-ligand complexes. the guanosine moiety of gpcu is also most pro ... | 1993 | 8104037 |
| adenophostins, newly discovered metabolites of penicillium brevicompactum, act as potent agonists of the inositol 1,4,5-trisphosphate receptor. | potent inositol 1,4,5-trisphosphate (ip3) receptor agonists, adenophostin a and b, were found from fungal products. in spite of the striking structural difference from ip3, adenophostins were found to inhibit [3h]ip3 binding more potently than ip3: the ki values for adenophostin a and b were both calculated to be 0.18 nm, while that of ip3 was 15 nm. adenophostins induced ca2+ release both from cerebellar microsomes and from intracellular ca2+ stores in permeabilized ng108-15 cells. adenophostin ... | 1994 | 8276820 |
| mushroom worker's lung: serologic reactions to thermophilic actinomycetes present in the air of compost tunnels. | vast numbers of spores of the thermophilic actinomycetes excellospora flexuosa, thermomonospora alba, t. curvata and t. fusca were collected from the air in fermentation tunnels during the spawning of mushroom compost, i.e. over 10(9) cfu m-3 of air. five different genera of fungi, namely, aspergillus, aureobasidium, cladosporium, penicillium and scytalidium, were found at only 10(3) cfu m-3 of air. agaricus bisporus, used for spawning, was absent. sera of 10 mushroom growers affected by mushroo ... | 1993 | 8326995 |
| production of feruloyl/rho-coumaroyl esterase activity by penicillium expansum, penicillium brevicompactum and aspergillus niger. | extracellular esterase production by penicillium expansum, penicillium brevicompactum and aspergillus niger was determined in both liquid and solid-state culture. methyl ferulate was used as the main carbon source in liquid culture whereas wheat bran and sugar beet pulp were used in solid-state culture. extracted enzyme for each fungus showed activity in the presence of onp butyrate, methyl ferulate, methyl coumarate and two 'natural' feruloylated carbohydrate esters. higher enzyme recoveries we ... | 1995 | 8557619 |
| penicillium brevicompactum as the cause of a necrotic lung ball in an allogeneic bone marrow transplant recipient. | the non-candida, non-aspergillus fungal infections are being reported with increasing frequency in bmt patients. one of these agents is penicillium which has rarely been implicated as a pathogen in these patients. only a few cases of isolated fungemias have been reported to date. we present the first documented case of invasive lung infection due to penicillium brevicompactum in an allogeneic bmt recipient. as this case shows, the diagnosis of non-candida, non-aspergillus fungal infections may b ... | 1996 | 8971395 |
| chlorine inactivation of fungal spores on cereal grains. | although 0.4% chlorine for 2 min has been recommended for surface disinfection of food samples before direct plating for fungal enumeration, this procedure may not be adequate for highly contaminated products. the effectiveness of a range of chlorine solutions was investigated using barley samples artificially contaminated with four different concentrations of aspergillus flavus. a. niger, a. ochraceus, eurotium repens, penicillium brevicompactum p. chrysogenum and cladosporium cladosporioides. ... | 1997 | 9105923 |
| effects of adenophostin-a and inositol-1,4,5-trisphosphate on cl- currents in xenopus laevis oocytes. | adenophostin-a, a novel compound isolated from cultures of penicillium brevicompactum, has been shown to stimulate ca2+ release from inositol-1,4,5-trisphosphate (ip3)-sensitive ca2+ stores in microsomal preparations, permeabilized cells, and lipid vesicles containing purified ip3 receptor. the purpose of the current study was to compare the effects of adenophostin-a and ip3 on ca2+ release from stores and ca2+ influx in intact xenopus laevis oocytes. ca2+ influx though store-operated ca2+ chann ... | 1997 | 9106635 |
| structural analogues of d-myo-inositol-1,4,5-trisphosphate and adenophostin a: recognition by cerebellar and platelet inositol-1,4,5-trisphosphate receptors. | adenophostins a and b, which are metabolic products of the fungus penicillium brevicompactum, are potent agonists at the d-myo-inositol-1,4,5-trisphosphate [ins(1,4,5)p3] receptor. in the current study, adenophostin a was approximately 50-fold more potent than ins(1,4,5)p3 at both releasing ca2+ from the intracellular stores of permeabilized platelets and displacing [3h]ins(1,4,5)p3 from its receptor on rat cerebellar membranes. various analogues bearing structural features found in the adenopho ... | 1997 | 9380038 |
| qualitative identification of volatile metabolites from two fungi and three bacteria species cultivated on two media. | two fungal species, aspergillus fumigatus and penicillium brevicompactum and three bacteria, klebsiella pneumoniae, enterobacter agglomerans and streptomyces albus were cultivated on two media, malt extract agar and dichloran glycerol agar. the volatile metabolite samples from the cultures were adsorbed on tenax ta and analyzed qualitatively by thermal desorption gas chromatography and with a mass selective detector. various hydrocarbons, alcohols, ketones, esters and terpenes were identified. t ... | 1998 | 9919382 |
| availability of phosphorus and sulfur of insecticide origin by fungi. | thirteen fungal species isolated from soil treated with pesticides were tested for their ability to mineralize and degrade three organophosphate insecticides currently used in egypt (cyolan, malathion and dursban) in liquid media free from phosphorus (p) and sulfur (s). all fungal species grew successfully on the culture media treated with the three used doses of insecticides (10, 50 and 100 ppm active ingredient) but the growth rate varied with the species, the insecticide and the doses. at 10 ... | 1998 | 10192894 |
| the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. | the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ... | 1999 | 10349723 |
| species-specific production of microbial volatile organic compounds (mvoc) by airborne fungi from a compost facility. | thirteen airborne fungal species frequently isolated in composting plants were screened for microbial volatile organic compounds (mvoc), i.e., aspergillus candidus, a. fumigatus, a. versicolor, emericella nidulans, paecilomyces variotii, penicillium brevicompactum, penicillium clavigerum, penicillium crustosum, penicillium cyclopium, penicillium expansum, penicillium glabrum, penicillium verruculosum, and tritirachium oryzae. air samples from pure cultures were sorbed on tenax gr and analyzed by ... | 1999 | 10448558 |
| synthesis and biological evaluation of new analogues of the active fungal metabolites n-(2-methyl-3-oxodecanoyl)-2-pyrroline and n-(2-methyl-3-oxodec-8-enoyl)-2-pyrroline. | to evaluate the effect of simplifying the beta-ketoamide system present in active isolated metabolites from penicillium brevicompactum (2 and 3) on the activity, new analogues with a monocarbonylic amide functionality have been obtained. in this way, the insecticidal and fungicidal activities have been improved in relation to the natural products taken as lead molecules. thus, two of the synthetic analogues (5a and 5b) showed very important insecticidal activities against third-instar nymphs of ... | 1999 | 10552736 |
| mycoflora and mycotoxins of brazilian cashew kernels. | kernel samples of common and dwarf brazilian cashew nuts were highly contaminated with field and storage fungi in comparison to healthy ones. in general, dwarf cashews were more contaminated than common. a total of 37 fungal species were identified. aspergillus niger was the dominant species with more colonies being isolated from dwarf kernels. a. flavus was the next most frequently isolated species. penicillium brevicompactum, and p. glabrum were the most frequently isolated penicillia, with hi ... | 1999 | 10598070 |
| igg antibodies, chronic bronchitis, and pulmonary function values in farmer's lung patients and matched controls. | we measured igg antibody levels against eight different microbes in farmer's lung (fl) patients an average of 14 years after the first diagnosed episode of fl and in matched controls. the study population consisted of 87 fl patients and 81 control farmers, matched by age, sex, and smoking habits. clinical studies included the measurement of igg antibody levels against absidia corymbifera, aspergillus umbrosus, a. fumigatus, humicola grisea, saccharopolyspora rectivirgula, penicillium brevicompac ... | 1999 | 10604554 |
| species-specific profiles of mycotoxins produced in cultures and associated with conidia of airborne fungi derived from biowaste. | the potential to produce mycotoxins and non-volatile secondary metabolites was investigated for approximately 250 freshly isolated fungal strains. among the eleven most relevant species, viz. aspergillus flavus, a. fumigatus, a. niger, a. parasiticus, a. versicolor, emericella nidulans, paecilomyces variotii, penicillium brevicompactum, p. clavigerum, p. crustosum, and p. polonicum, a wide range of metabolites partly of toxicological relevance was identified. several unknown metabolites were fou ... | 2000 | 11109562 |
| performance of air-o-cell, burkard, and button samplers for total enumeration of airborne spores. | performance of three devices used for the total enumeration of airborne spores-the air-o-cell sampling cassette, the burkard personal volumetric air sampler, and the button aerosol sampler--was evaluated under controlled laboratory conditions. the first two are glass-slide impactors; the third collects spores on a filter. the samplers were challenged with 0.44-5.10 microm polystyrene latex particles and five microorganisms of 0.84-3.07 microm mean aerodynamic diameter: streptomyces albus, bacill ... | 2000 | 11192220 |
| mycoflora and mycotoxins in brazilian black pepper, white pepper and brazil nuts. | a wide range of field and storage fungi were isolated from black pepper, white pepper and brazil nut kernels from amazonia. a total of 42 species were isolated from both peppers. aspergillus flavus and a. niger were isolated more frequently from black than from white pepper. other potential mycotoxigenic species isolated included: a. ochraceus, a. tamarii, a. versicolor, emericella nidulans and chaetomium globosum, penicillium brevicompactum, p. citrinum, p. islandicum and p. glabrum. species is ... | 2000 | 11229375 |
| detection of microbial volatile organic compounds (mvocs) produced by moulds on various materials. | twelve fungal species were screened for microbial volatile organic compounds (mvocs): aspergillus fumigatus, a. versicolor, a. niger, a. ochraceus, trichoderma harzianum, t. pseudokoningii, penicillium brevicompactum, p. chrysogenum, p. claviforme, p. expansum, fusarium solani and mucor sp. more than 150 volatile substances derived from fungal cultures have been analysed by head-space solid-phase microextraction (hs-spme). each species had a defined mvoc profile which may be subjected to conside ... | 2001 | 11759153 |
| limitations of monoclonal antibodies for monitoring of fungal aerosols using penicillium brevicompactum as a model fungus. | molds are ubiquitous in every environment and many species have been recently associated with an increase in opportunistic infections in immunocompromised patients or the exacerbation of asthmatic episodes in allergic patients. the degree of environmental contamination with fungi thus needs to be monitored and in this study we report the development of a monoclonal antibody (mab)-mediated enzyme-linked immunosorbent assay (elisa) for the detection of spores of penicillium brevicompactum in exper ... | 2003 | 14659915 |
| the presence of penicillium and penicillium mycotoxins in food wastes. | a total of 97 samples (48 summer and 49 winter) of food waste from private households were investigated for penicillium and for mycotoxins. twenty-five penicillium species were isolated and penicillium crustosum, penicillium brevicompactum, penicillium chrysogenum, penicillium expansum, penicillium roqueforti, penicillium spinulosum, penicillium viridicatum, penicillium commune, penicillium citrinum and penicillium solitum were, in decreasing order, the most frequently identified species. mycoto ... | 2004 | 14698099 |
| development and use of flow cytometry for detection of airborne fungi. | traditional methods for the enumeration of airborne fungi are slow, tedious, and rather imprecise. in this study, the possibility of using flow cytometry (fcm) for the assessment of exposure to the fungus aerosol was evaluated. epifluorescence microscopy direct counting was adopted as the standard for comparison. setting up of the method was achieved with pure suspensions of aspergillus fumigatus and penicillium brevicompactum conidia at different concentrations, and then analyses were extended ... | 2004 | 15006754 |
| mould contamination in production of semi-hard cheese. | air, equipment, plastic film, brine and milk were sampled from four cheese factories in norway during the period september 1997 to may 1999 in order to identify the critical points for mould contamination in the production process. altogether, 672 samples were collected. penicillium brevicompactum was the most frequently isolated species from three of the factories, while geotrichum candidum was the most frequently isolated species from the fourth. p. commune, p. palitans, p. solitum and p. roqu ... | 2004 | 15135581 |
| multilocus dna sequence analysis shows that penicillium biourgeianum is a distinct species closely related to p. brevicompactum and p. olsonii. | penicillium brevicompactum and other isolates with the compact, complex conidiogenous apparatus typical of the species were sequenced in one ribosomal and two protein coding regions. the aligned dna sequences were analyzed by maximum parsimony and the data from different loci were tested for compatibility using the partition homogeneity test. analysis of each of the three loci revealed three clades corresponding to p. brevicompactum, p. olsonii and p. biourgeianum. using the phylogenetic species ... | 2004 | 15209283 |
| methods to maximise the staining of fungal propagules with fluorescent dyes. | the spores and conidia of most fungi have very thick and resistant cell walls that severely impede the staining with fluorescent dyes to allow epifluorescence microscopy to be employed for their direct detection and quantification in natural habitats. in this study, oxidation by sodium hypochlorite and microwave irradiation (mwi) were used to enhance the staining of aspergillus fumigatus and penicillium brevicompactum conidia with six fluorescent dyes. sodium hypochlorite resulted in high percen ... | 2004 | 15488280 |
| mites and fungi in heavily infested stores in the czech republic. | toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ... | 2004 | 15666776 |
| the isoepoxydon dehydrogenase gene of patulin biosynthesis in cultures and secondary metabolites as candidate pcr inhibitors. | the european union (eu) has introduced statutory limits for patulin in fruit products. species definitions need to be unambiguous for this capability, especially to determine 'weak spots' in food, drink and feed production. fungi were analysed for the isoepoxydon dehydrogenase (idh) gene of the patulin metabolic pathway to indicate potential patulin production. in several cases inhibition of pcr product formation was indicated. dilution as a means of overcoming pcr inhibition and to determine op ... | 2004 | 15757179 |
| mycotoxin production and postharvest storage rot of ginger (zingiber officinale) by penicillium brevicompactum. | twenty naturally infected ginger (zingiber officinale) rhizomes displaying visible mold growth were examined to identify the fungi and to evaluate the presence of fungal secondary metabolites. penicillium brevicompactum was the predominant species isolated from 85% of the samples. mycophenolic acid was identified from corresponding tissue extracts. because mycophenolic acid is a potent immunosuppressant and synergistic mycotoxicosis studies involving human consumption have not been carried out o ... | 2005 | 15771190 |
| inflammatory and cytotoxic responses in mouse lungs exposed to purified toxins from building isolated penicillium brevicompactum dierckx and p. chrysogenum thom. | in vitro and in vivo studies have shown that building-associated penicillium spores and spore extracts can induce significant inflammatory responses in lung cells and animal models of lung disease. however, because spores and spore extracts comprise mixtures of bioactive constituents often including toxins, it is impossible to resolve which constituent mediates inflammatory responses. this study examined dose-response (0.5 nm, 2.5 nm, 5.0 nm, 12.5 nm/g body weight (bw) animal) and time-course (3 ... | 2005 | 15958659 |
| novel inhibitors of the mitochondrial respiratory chain: oximes and pyrrolines isolated from penicillium brevicompactum and synthetic analogues. | the capacity of inhibition of the mammalian mitochondrial respiratory chain of brevioxime 5a, a natural insecticide compound isolated from penicillium brevicompactum culture broth, and another 15 analogue compounds, other oximes 5b and 5c; two diastereomeric pyrrolidines 1c' and 1c' '; five pyrrolines 3c', 3c' ' (diastereomers between them), 3a, 3b, and 6; two oxazines 4c' and 4c' ' (also diastereomers between them); and four pyrrol derivatives 7-10, are analyzed in this paper. compounds 3b, 3c' ... | 2005 | 16218678 |
| toxic micromycetes in grain raw material during its processing. | in 2003-2005 micromycetes were isolated and identified from wheat, barley, rye, buckwheat grain brought into mills or from processing enterprises. contamination of the produced flour with micromycete propagules (cfu g(-1)), changes in micromycete diversity and abundance in the course of flour storage, preparation and baking of bread, production of groats or other food products and fodder were determined. most attention was given to widely distributed micromycetes, known producers of toxins: alte ... | 2006 | 16841886 |
| antimicrobial and cytotoxic knotwood extracts and related pure compounds and their effects on food-associated microorganisms. | knotwood or bark extracts prepared from 30 species of hard and soft wood trees as well as selected pure compounds (lignans, stilbenes and flavonoids) were assayed for their antimicrobial activity against a battery of both gram positive and negative bacteria, yeasts, and filamentous fungi (bacillus cereus, staphylococcus aureus, listeria monocytogenes, lactobacillus plantarum, escherichia coli, salmonella infantis, pseudomonas fluorescens, candida albicans, saccharomyces cerevisiae, aspergillus f ... | 2007 | 17188387 |
| fungi in bottled water: a case study of a production plant. | a one-year fungal survey of a water bottling plant was conducted in order to evaluate the incidence and fluctuations of the mycobiota. the dominant fungal genera in order of highest numbers isolated were penicillium, cladosporium and trichoderma followed by aspergillus, paecilomyces, and others. as expected, the highest number of isolates were collected during the warmer months, particularly may and june. indeed during these two months there were more fungi present in the water, indicating that ... | 2006 | 17196019 |
| polymerase chain reaction (pcr) identification of penicillium brevicompactum, a grape contaminant and mycophenolic acid producer. | penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. the present work has obtained evidence, by liquid chromatography (lc)-ion-trap mass spectrometry, of the ability of p. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. in order to facilitate early diagnosis of this species on commodities for human and animal consumption, ... | 2007 | 17364917 |
| fungal pneumonia caused by penicillium brevicompactum in a young staffordshire bull terrier. | | 2007 | 17468325 |
| combinations of antimycotics to inhibit the growth of molds capable of producing 1,3-pentadiene. | some species of molds are capable of degrading sorbic acid to produce 1,3-pentadiene, a volatile compound with an unpleasant hydrocarbon-like odor. the effectiveness of reduced concentrations of sorbate, in combination with other antimycotics, to control the growth of these molds has not been described. we did a study to evaluate potassium sorbate, sodium benzoate, calcium propionate, disodium ethylenediaminetetraacetic acid (edta), and natamycin, alone and in combination, for their effectivenes ... | 2008 | 17993388 |
| use of (1-3)-beta-d-glucan concentrations in dust as a surrogate method for estimating specific fungal exposures. | indoor exposure to fungi has been associated with respiratory symptoms,often attributed to their cell wall component, (1-3)-beta-d-glucan. performing(1-3)-beta-d-glucan analysis is less time consuming and labor intensive than cultivation or microscopic counting of fungal spores. this has prompted many to use(1-3)-beta-d-glucan as a surrogate for fungal exposure. the aim of this study was to examine which indoor fungal species are major contributors to the (1-3)-beta-d-glucan concentration in fie ... | 2008 | 18429996 |
| fungal flora in indoor and outdoor air of different residential houses in tekirdag city (turkey): seasonal distribution and relationship with climatic factors. | this study was investigated the density and monthly distribution of indoor and outdoor microfungi in six different residential houses in tekirdag city through the exposure of petri dishes containing rose-bengal streptomycin agar media. samples were collected in 1-month intervals over a period of 12 months between march, 2001, and february, 2002. we used 432 petri dishes and counted a total of 4,205 microfungi colonies, 1,790 from indoor air and 2,415 from outdoor air. as a result, 42 species bel ... | 2009 | 18509736 |
| mold species in dust from the international space station identified and quantified by mold-specific quantitative pcr. | dust was collected over a period of several weeks in 2007 from hepa filters in the u.s. laboratory module of the international space station (iss). the dust was returned on the space shuttle atlantis, mixed, sieved and the dna was extracted. using a dna-based method called mold-specific quantitative pcr (msqpcr), 39 molds were measured in the dust. potential opportunistic pathogens aspergillus flavus and aspergillus niger and potential moderate toxin producers penicillium chrysogenum and penicil ... | 2008 | 18602989 |
| field evaluation of a personal, bioaerosol cyclone sampler. | a personal cyclone sampler (cyclone) was operated continuously alongside a 25-mm filter sampler (filter), a slit impactor (burkard slide), and a high-volume cyclone sampler (burkard cyclone) at an outdoor location with abundant naturally occurring fungi (n = 30; sampling time: 12.5 +/- 2.3 hr). air concentrations (spore m(-3)) of 28 fungal groups were determined for all samplers by microscopy. cyclone performance was judged using various indices to determine if it agreed with the other samplers ... | 2008 | 18780236 |
| biotransformation of the marine sesquiterpene pacifenol by a facultative marine fungus. | continued investigation of the marine alga laurencia claviformis has led to the isolation of pacifenol, a halogenated sesquiterpene as the major metabolite. the microbial transformation of pacifenol is reported. it was cultivated with a marine strain of penicillium brevicompactum yielding a new compound. the structure was elucidated on the basis of spectroscopic data. the anti-microbial activity of pacifenol derivatives is reported. | 2008 | 19085420 |
| evaluation of quantitative pcr and culture methods for detection of house dust fungi and streptomycetes in relation to moisture damage of the house. | microbial concentrations in vacuumed house dust samples (n = 71) were analysed by culture and quantitative polymerase chain reaction (qpcr) methods and their association with extent of moisture damage in the house was studied. | 2008 | 19241524 |
| expression and characterization of pen b 26 allergen of penicillium brevicompactum in escherichia coli. | pen b 26 is one of the allergens produced by penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. pen b 26 wa scloned and expressed as an n-terminal as well as a c-terminal his6 tagged fusion protein in escherichia coli. this allergen was purified by immobilized ni2+-affinity chromatography. the purified pen b 26 was characterized by immunological, biochemical and biophysical methods. c-his6 tagged pen ... | 2009 | 19248220 |
| determination of microbial volatile organic compounds (mvocs) by passive sampling onto charcoal sorbents. | passive sampling combined with gc-ms was applied for the detection of microbial volatile organic compounds (mvocs) emitted by mould cultures. the fungi species penicillium expansum, penicillium chrysogenum, penicillium brevicompactum, aspergillus fumigatus, aspergillus versicolor, aspergillus niger, and cladosporium cladosporoides were cultivated on dichloran glycerol agar. the emitted volatiles were collected by charcoal-based passive diffusion monitors during the first four weeks of growth. se ... | 2009 | 19289243 |
| the mycobiota of three dry-cured meat products from slovenia. | the surface mycobiota of three types of slovenian dry-cured meat products were isolated from a total of 75 items of product that were sampled periodically during the drying/ripening stage of processing. the predominant filamentous fungal genus isolated was penicillium. eurotium spp., aspergillus versicolor and cladosporium spp. were isolated from only two of the products. eight penicillium species were identified. penicillium nordicum was recovered frequently. penicillium nalgiovense was recover ... | 2010 | 21356440 |
| versatile enzyme expression and characterization system for aspergillus nidulans, with the penicillium brevicompactum polyketide synthase gene from the mycophenolic acid gene cluster as a test case. | assigning functions to newly discovered genes constitutes one of the major challenges en route to fully exploiting the data becoming available from the genome sequencing initiatives. heterologous expression in an appropriate host is central in functional genomics studies. in this context, filamentous fungi offer many advantages over bacterial and yeast systems. to facilitate the use of filamentous fungi in functional genomics, we present a versatile cloning system that allows a gene of interest ... | 2011 | 21398493 |
| adaptive evolution of drug targets in producer and non-producer organisms. | mpa (mycophenolic acid) is an immunosuppressive drug produced by several fungi in penicillium subgenus penicillium. this toxic metabolite is an inhibitor of impdh (imp dehydrogenase). the mpa-biosynthetic cluster of penicillium brevicompactum contains a gene encoding a b-type impdh, impdh-b, which confers mpa resistance. surprisingly, all members of the subgenus penicillium contain genes encoding impdhs of both the a and b types, regardless of their ability to produce mpa. duplication of the imp ... | 2012 | 21916847 |
| properties of the bubble protein, a defensin and an abundant component of a fungal exudate. | colonies of the ascomycete fungus penicillium brevicompactum dierckx produce bright yellow-green fluorescent exudate bubbles on its surface when grown on standard plant cell culture medium. according to sds-page analysis, the exudate is enriched in one protein, named bubble protein (bp). detailed characteristics of bp are described, and also its corresponding genomic promoter and terminator sequences that flank sequences encoding signal peptide and a precursor sequence upstream of that of the ma ... | 2011 | 21906643 |
| a new class of imp dehydrogenase with a role in self-resistance of mycophenolic acid producing fungi. | many secondary metabolites produced by filamentous fungi have potent biological activities, to which the producer organism must be resistant. an example of pharmaceutical interest is mycophenolic acid (mpa), an immunosuppressant molecule produced by several penicillium species. the target of mpa is inosine-5'-monophosphate dehydrogenase (impdh), which catalyses the rate limiting step in the synthesis of guanine nucleotides. the recent discovery of the mpa biosynthetic gene cluster from penicilli ... | 2011 | 21923907 |
| surface hydrophobicity of culture and water biofilm of penicillium spp. | fungal surface hydrophobicity is involved in several functions in fungal growth and development. water contact angles measurement has been used as a direct and simple approach for its characterisation in solid cultures. microsphere adhesion assay is said to be the best method to assess cell hydrophobicity of filamentous fungi. this study aimed to apply these two methods to study hydrophobicity of penicillium expansum and penicillium brevicompactum grown as mycelial mats in solid culture, liquid ... | 2011 | 22011877 |
| microbial synthesis of gold nanoparticles using the fungus penicillium brevicompactum and their cytotoxic effects against mouse mayo blast cancer c 2 c 12 cells. | microorganisms, their cell filtrates, and live biomass have been utilized for synthesizing various gold nanoparticles. the shape, size, stability as well as the purity of the bio synthesized nanoparticles become very essential for application purpose. in the present study, gold nanoparticles have been synthesized from the supernatant, live cell filtrate, and biomass of the fungus penicillium brevicompactum. the fungus has been grown in potato dextrose broth which is also found to synthesize gold ... | 2011 | 21894479 |
| [properties and specificity of the extracellular acid ribonuclease of penicillium brevicompactum]. | | 2016 | 4791847 |
| mycophenolic acid production by penicillium brevicompactum in two media. | penicillium brevicompactum produces mycophenolic acid as it grows vegetatively, not only on a simple medium where growth is slow but also on a richer medium where growth is less restricted. the implications of this finding on the association of fungal secondary metabolism with the idiophase in liquid and solid culture are discussed. | 1990 | 526891 |
| [gradual synthesis of oligonucleotides. the specificity of the synthetic function of penicillium brevicompactum ribonuclease and the structure of the phosphate acceptor]. | | 2015 | 4352774 |
| characterization of in vitro antifungal activities of small and american cranberry (vaccinium oxycoccos l. and v. macrocarpon aiton) and lingonberry (vaccinium vitis-idaea l.) concentrates in sugar reduced fruit spreads. | in this study, cranberry and lingonberry concentrates were added to commercial sugar-reduced fruit spreads (raspberry-aloe vera, strawberry-guava, and strawberry-lime), and tested for their antifungal activities. selected strains of the species absidia glauca, penicillium brevicompactum, saccharomyces cerevisiae and zygosaccharomyces bailii, as well as xerophilic environmental isolates of the genera penicillium and eurotium were used for challenge testing. initially, varying concentrations of sy ... | 2015 | 25868124 |
| molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time pcr. | microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction (pcr). a total of 37,191 high quality sequences were obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. the shannon diversity index for archaea, bacteria and fungi was respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioae ... | 2015 | 25872733 |
| insertion mutation in hmg-coa lyase increases the production yield of mpa through agrobacterium tumefaciens-mediated transformation. | mycophenolic acid (mpa) is an antibiotic produced by penicillium brevicompactum. mpa has antifungal, antineoplastic, and immunosuppressive functions, among others. β-hydroxy-β-methylglutaryl-coa (hmg-coa) lyase is a key enzyme in the bypass metabolic pathway. the inhibitory activity of hmg-coa lyase increases the mpa biosynthetic flux by reducing the generation of by-products. in this study, we cloned the p. brevicompactum hmg-coa lyase gene using the thermal asymmetric interlaced polymerase cha ... | 2016 | 27558440 |
| some chronic rhinosinusitis patients have elevated populations of fungi in their sinuses. | to measure the populations of 36 fungi in the homes and sinuses of chronic rhinosinusitis (crs) and non-crs patients. | 2012 | 22532289 |
| plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations. | few studies describing eukaryotic communities in the human gut microbiota have been published. the objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient. | 2013 | 23555039 |
| occurrence of moulds from bee pollen in central italy--a preliminary study. | the present study aimed to preliminary evaluate the occurrence of fungi in 40 specimens of trapped pollen collected from april-september 2015 in 40 apiaries from tuscany (central italy). cultural and microscopical examinations allowed the recognition of cladosporium sp., alternaria sp., humicola sp. mucoraceae and acremonium sp. penicillium brevicompactum, penicillium chrysogenum, aspergillus flavus , aspergillus nidulans, aspergillus niger and aspergillus terreus were also identified. yeasts an ... | 2016 | 27010410 |
| assessment of multi-contaminant exposure in a cancer treatment center: a 2-year monitoring of molds, mycotoxins, endotoxins, and glucans in bioaerosols. | indoor air quality in health care facilities is a major public health concern, particularly for immunocompromised patients who may be exposed to microbiological contaminants such as molds, mycotoxins, endotoxins, and (1,3)-ß-d-glucans. over 2 years, bioaerosols were collected on a monthly basis in a cancer treatment center (centre f. baclesse, normandy, france), characterized from areas where there was no any particular air treatment. results showed the complexity of mycoflora in bioaerosols wit ... | 2017 | 28012082 |
| antifungal properties of silver nanoparticles against indoor mould growth. | the presence of moulds in indoor environments causes serious diseases and acute or chronic toxicological syndromes. in order to inhibit or prevent the growth of microorganisms on building materials, the disruption of their vital processes or the reduction of reproduction is required. the development of novel techniques that impair the growth of microorganisms on building materials is usually based on silver nanoparticles (agnps). it makes them an alternative to other biocides. agnps have proven ... | 2015 | 25847174 |
| generation and characterization of indoor fungal aerosols for inhalation studies. | in the indoor environment, people are exposed to several fungal species. evident dampness is associated with increased respiratory symptoms. to examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. the aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. indoor airborne fungi were sampled ... | 2016 | 26921421 |
| involvement of a natural fusion of a cytochrome p450 and a hydrolase in mycophenolic acid biosynthesis. | mycophenolic acid (mpa) is a fungal secondary metabolite and the active component in several immunosuppressive pharmaceuticals. the gene cluster coding for the mpa biosynthetic pathway has recently been discovered in penicillium brevicompactum, demonstrating that the first step is catalyzed by mpac, a polyketide synthase producing 5-methylorsellinic acid (5-moa). however, the biochemical role of the enzymes encoded by the remaining genes in the mpa gene cluster is still unknown. based on bioinfo ... | 2012 | 22544261 |
| does spore count matter in fungal allergy?: the role of allergenic fungal species. | fungi have been known to be important aeroallergens for hundreds of years. most studies have focused on total fungal concentration; however, the concentration of specific allergenic fungi may be more important on an individual basis. | 2016 | 27334778 |
| degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures. | contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating european water resources. the aim of this work was to evaluate the ability of the fungi fusarium oxysporum, aspergillus oryzae, lentinula edodes, penicillium brevicompactum and lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. these pesticides ar ... | 2012 | 22878100 |
| diversity and bioprospection of fungal community present in oligotrophic soil of continental antarctica. | we surveyed the diversity and capability of producing bioactive compounds from a cultivable fungal community isolated from oligotrophic soil of continental antarctica. a total of 115 fungal isolates were obtained and identified in 11 taxa of aspergillus, debaryomyces, cladosporium, pseudogymnoascus, penicillium and hypocreales. the fungal community showed low diversity and richness, and high dominance indices. the extracts of aspergillus sydowii, penicillium allii-sativi, penicillium brevicompac ... | 2015 | 25809294 |
| release and characteristics of fungal fragments in various conditions. | intact spores and submicrometer size fragments are released from moldy building materials during growth and sporulation. it is unclear whether all fragments originate from fungal growth or if small pieces of building materials are also aerosolized as a result of microbial decomposition. in addition, particles may be formed through nucleation from secondary metabolites of fungi, such as microbial volatile organic compounds (mvocs). in this study, we used the elemental composition of particles to ... | 2016 | 26789361 |
| effects of fungal species, cultivation time, growth substrate, and air exposure velocity on the fluorescence properties of airborne fungal spores. | real-time bioaerosol monitoring is possible with fluorescence based instruments. this study provides information on major factors that can affect the fluorescence properties of airborne fungal spores. two fluorescence-based bioaerosol detectors, bioscout, and ultraviolet aerodynamic particle sizer (uvaps), were used to study fluorescent particle fractions (fpfs) of released spores of three fungal species (aspergillus versicolor, cladosporium cladosporioides, and penicillium brevicompactum). two ... | 2015 | 25292152 |
| susceptibility of green and conventional building materials to microbial growth. | green building materials are becoming more popular. however, little is known about their ability to support or limit microbial growth. the growth of fungi was evaluated on five building materials. two green, two conventional building materials and wood as a positive control were selected. the materials were inoculated with aspergillus versicolor, cladosporium cladosporioides and penicillium brevicompactum, in the absence and presence of house dust. microbial growth was assessed at four different ... | 2015 | 24975616 |
| protease and lipase activities of fungal and bacterial strains derived from an artisanal raw ewe's milk cheese. | we previously identified the microbiota present during cheese ripening and observed high protease and lipase activity in divle cave cheese. to determine the contribution of individual isolates to enzyme activities, we investigated a range of species representing this microbiota for their proteolytic and lipolytic ability. in total, 17 fungal, 5 yeast and 18 bacterial strains, previously isolated from divle cave cheese, were assessed. qualitative protease and lipase activities were performed on s ... | 2016 | 27541978 |
| detection of volatile metabolites of moulds isolated from a contaminated library. | the principal fungal species isolated from a contaminated library environment were tested for their microbial volatile organic compound (mvoc) production ability. aspergillus creber, a. penicillioides, cladosporium cladosporioides, eurotium chevalieri, e. halophilicum, penicillium brevicompactum and p. chrysogenum were cultivated on suitable culture media inside sample bottles specifically designed and created for direct mvoc injection to a gc-ms instrument. the fungal emissions were monitored o ... | 2016 | 27392938 |
| isolation, expression and characterization of a minor allergen from penicillium crustosum. | a ribosomal p1 protein, pen b 26 from penicillium brevicompactum, was previously identified as a major allergen. a homolog protein was isolated and characterized from penicillium crustosum which is not known to be allergenic mold. a cdna library of p. crustosum was constructed and screened using a probe based on the dna sequence of pen b 26. a positive clone was isolated, expressed in escherichia coli, purified and characterized by comparing its immunological and physical properties to pen b 26. ... | 2014 | 23902156 |
| identification of antifungal substances of lactobacillus sakei subsp. ali033 and antifungal activity against penicillium brevicompactum strain fi02. | this study was performed to investigate the antifungal substances and the antifungal activity against fungi of lactic acid bacteria (lab) isolated from kimchi. lab from kimchi in imsil showed antifungal activity against penicillium brevicompactum strain fi02. lab li031 was identified as lactobacillus sakei subsp. antifungal substances contained in l. sakei subsp. ali033 culture media were unstable at high ph levels. both, the control and proteinase k and protease treated samples showed clear zon ... | 2016 | 27069906 |
| the root growth-regulating brevicompanine natural products modulate the plant circadian clock. | plant growth regulating properties of brevicompanines (brvs), natural products of the fungus penicillium brevicompactum, have been known for several years, but further investigations into the molecular mechanism of their bioactivity have not been performed. following chemical synthesis of brevicompanine derivatives, we studied their activity in the model plant arabidopsis by a combination of plant growth assays, transcriptional profiling and numerous additional bioassays. these studies demonstra ... | 2017 | 28379676 |
| production of mycophenolic acid by penicillium brevicompactum-a comparison of two methods of optimization. | production of mycophenolic acid (mpa) by submerged fermentation using the microfungus penicillium brevicompactum mtcc 8010 is reported here. screening experiments were used to identify: the suitable media composition; the optimal initial ph; and the optimal incubation temperature to maximize the production of mpa in batch cultures. the initial concentrations of the selected sources of carbon (glucose), nitrogen (peptone) and the precursors (methionine, glycine) were then optimized by: (1) one-at ... | 2016 | 28352543 |
| brevianamides and mycophenolic acid derivatives from the deep-sea-derived fungus penicillium brevicompactum dffscs025. | four new compounds (1-4), including two brevianamides and two mycochromenic acid derivatives along with six known compounds were isolated from the deep-sea-derived fungus penicillium brevicompactum dffscs025. their structures were elucidated by spectroscopic analysis. moreover, the absolute configurations of 1 and 2 were determined by quantum chemical calculations of the electronic circular dichroism (ecd) spectra. compound 9 showed moderate cytotoxicity against human colon cancer hct116 cell li ... | 2017 | 28218640 |
| genetic basis for mycophenolic acid production and strain-dependent production variability in penicillium roqueforti. | mycophenolic acid (mpa) is a secondary metabolite produced by various penicillium species including penicillium roqueforti. the mpa biosynthetic pathway was recently described in penicillium brevicompactum. in this study, an in silico analysis of the p. roqueforti fm164 genome sequence localized a 23.5-kb putative mpa gene cluster. the cluster contains seven genes putatively coding seven proteins (mpaa, mpab, mpac, mpade, mpaf, mpag, mpah) and is highly similar (i.e. gene synteny, sequence homol ... | 2017 | 27889155 |
| production of mycophenolic acid by penicillium brevicompactum using solid state fermentation. | solid-state fermentation using the microfungus penicillium brevicompactum for the production of mycophenolic acid is reported in this paper. of the initial substrates tested (whole wheat, cracked wheat, long grain basmati rice, and short grain parmal rice), parmal rice proved to be the best. under initial conditions, using steamed parmal rice with 80% (w/w) initial moisture content, a maximum mycophenolic acid concentration of 3.4 g/kg substrate was achieved in 12 days of fermentation at 25 °c. ... | 2016 | 27844340 |
| purification and characterization of the enzymes involved in nicotinamide adenine dinucleotide degradation by penicillium brevicompactum nrc 829. | the present study was conducted to investigate a new pathway for the degradation of nicotinamide adenine dinucleotide (nad) by penicillium brevicompactum nrc 829 extracts. enzymes involved in the hydrolysis of nad, i.e. alkaline phosphatase, aminohydrolase and glycohydrolase were determined. alkaline phosphatase was found to catalyse the sequential hydrolysis of two phosphate moieties of nad molecule to nicotinamide riboside plus adenosine. adenosine was then deaminated by aminohydrolase to inos ... | 2016 | 28330104 |
| characterization of a farnesyl diphosphate synthase gene from penicillium brevicompactum mucl 19011. | farnesyl diphosphate synthase is a critical enzyme in the isoprenoids biosynthesis pathway responsible for ergosterol and secondary metabolites biosynthesis in fungi. | 2016 | 26334936 |
| antifungal activity of essential oils against selected terverticillate penicillia. | the aim of this study was to screen 15 essential oils of selected plant species, viz. lavandula angustifolia, carum carvi, pinus mungo var. pulmilio, mentha piperita, chamomilla recutita l., pinus sylvestris, satureia hortensis l., origanum vulgare l., pimpinella anisum, rosmarinus officinalis l., salvia officinalis l., abietis albia etheroleum, chamomilla recutita l. rausch, thymus vulgaris l., origanum vulgare l. for antifungal activity against five penicillium species: penicillium brevicompac ... | 2015 | 25780826 |
| functional characterization of mpag', the o-methyltransferase involved in the biosynthesis of mycophenolic acid. | mycophenolic acid (mpa, 1) is a clinically important immunosuppressant. in this report, a gene cluster mpa' responsible for the biosynthesis of 1 was identified from penicillium brevicompactum nrrl 864. the s-adenosyl-l-methionine-dependent (sam-dependent) o-methyltransferase encoded by the mpag' gene was functionally and kinetically characterized in vitro. mpag' catalyzes the methylation of demethylmycophenolic acid (dmmpa, 6) to form 1. it also showed significant substrate flexibility by methy ... | 2015 | 25630520 |
| fungal endophyte diversity and bioactivity in the mediterranean cypress cupressus sempervirens. | fungal endophytes were isolated from the mediterranean cypress cupressus sempervirens. eleven taxa of fungi, all within the ascomycota, were identified based on pcr amplification and sequencing of the internal transcribed spacer sequences of nuclear ribosomal dna (its rdna) with taxonomic identity assigned using the ncbi nucleotide megablast search tool. the endophytic fungi included alternaria multiformis, didymella sp., phoma sp., phoma herbarum, pyrenochaeta sp. (dothideomycetes), penicillium ... | 2015 | 25527365 |
| detection and identification of xerophilic fungi in belgian chocolate confectionery factories. | chocolate confectionery fillings are generally regarded as microbiologically stable. the stability of these fillings is largely due to the general practice of adding either alcohol or preservatives. consumer demands are now stimulating producers to move away from adding alcohol or other preservatives to their confectionery fillings and instead to search for innovative formulations. such changes in composition can influence the shelf life of the product and may lead to spoilage by xerophilic fung ... | 2015 | 25475302 |
| penicillium strains isolated from slovak grape berries taxonomy assessment by secondary metabolite profile. | the secondary metabolite profiles of microfungi of the genus penicillium isolated from samples of grape berries collected in two different phases during two vegetative seasons in slovakia is described to assess the taxonomy. three slovak vine regions have been selected for this study, based on their climatic differences and national economic importance. cultures of microfungi isolated from berries were incubated on different selective media for macro and micromorphology identification. the speci ... | 2014 | 25109845 |
| assessing a mixture of biosurfactant and enzyme pools in the anaerobic biological treatment of wastewater with a high-fat content. | the use of rhamnolipid-type biosurfactant produced by pseudomonas aeruginosa was evaluated for solubilization of fat present in effluent from a poultry processing plant, followed by anaerobic biological treatment. the rhamnolipid was used in combination with enzyme pools produced by solid medium fermentation of the fungi penicillium simplicissimum and penicillium brevicompactum. in experiments with raw effluent, the accumulation of fat caused the specific methane production (smp) to be much less ... | 2014 | 24956798 |
| [establishment of a genetic transformation system for penicillium brevicompactum to produce mycophenolic acid]. | we established a genetic transformation system for penicillium brevicompactum to produce mycophenolic acid. | 2013 | 24617265 |
| two new penicillium species penicillium buchwaldii and penicillium spathulatum, producing the anticancer compound asperphenamate. | penicillium buchwaldii sp. nov. (type strain cbs 117181(t) = ibt 6005(t) = imi 30428(t) ) and penicillium spathulatum sp. nov. (cbs 117192(t) = ibt 22220(t) ) are described as new species based on a polyphasic taxonomic approach. isolates of p. buchwaldii typically have terverticillate conidiophores with echinulate thick-walled conidia and produce the extrolites asperphenamate, citreoisocoumarin, communesin a and b, asperentin and 5'-hydroxy-asperentin. penicillium spathulatum is unique in ha ... | 2013 | 23173673 |
| applications of ultrasound to enhance mycophenolic acid production. | reducing production costs for fermentation-based drugs (e.g., antibiotics) is crucial for the long-term sustainability of healthcare. in this study, we propose a novel low-intensity pulsed ultrasound (lipus) stimulation scheme using a nominal frequency of 1.5 mhz with a 20% duty cycle (200 μs ultrasound on and 800 μs ultrasound off) to increase production of fermentation-based drugs. we chose penicillium brevicompactum as a model system to demonstrate the performance of our lipus system. penicil ... | 2012 | 22749819 |
| microscopic fungi recovered from honey and their toxinogenity. | the objective of this investigation was to contribute towards the knowledge of microbiology of honey, more than 50 samples of honey from slovakia and other countries were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi from penicillium genera. the study revealed that out of 13 genera recovered, penicillium was the most frequent and diverse genus, followed by aspergillus and cladosporium being found in 65.91 % (29 samples), 34.1 % ... | 2012 | 22702826 |