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molecular cloning of the glucoamylase gene of aspergillus shirousami and its expression in aspergillus oryzae.the glucoamylase enzyme (gaase) gene from aspergillus shirousami was cloned and sequenced from genomic and cdna libraries. the genomic gene was located in the 5.4 kb ecori fragment. the deduced amino acid sequence of gaase contained 639 amino acid residues with a relative molecular mass of approximately 68,000 daltons (non-glycosylated form). the genomic gene of a. shirousami gaase was introduced into aspergillus oryzae. these transformants had increased gaase and raw starch degradation (rsd) ac ...19901368603
isolation and sequencing of a new glucoamylase gene from an aspergillus niger aggregate strain (dsm 823) molecularly classified as aspergillus tubingensis.based on morphological characteristics the taxa included in the aspergillus aggregate can hardly be differentiated. for that reason the phylogeny of this genus was revised several times as different criteria, from morphological to later molecular, were used. we found, comparing nucleotide sequences of the its-region, that the strain aspergillus niger (dsm 823) which is claimed to be identical to the strains atcc 10577, imi 027809, nctc 7193 and nrrl 2322 can be molecularly classified as aspergil ...200516284933
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