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expression and sequence comparison of the aspergillus niger and aspergillus tubigensis genes encoding polygalacturonase ii.the structure and expression of the polygalacturonase-encoding pgaii genes of two recently recognized species, aspergillus niger and aspergillus tubigensis, was investigated. while the structure of the pgaii genes is very similar, showing 83% dna sequence identity and 94% identity at the amino acid level, they have diverged significantly. the nh2-terminal sequence suggests that these pgs are made as pre pro-proteins and the secretory propeptide of the pgii precursors shows sequence homology with ...19911878999
mitochondrial dna restriction fragment length polymorphisms in field isolates of the aspergillus niger aggregate.the mitochondrial dnas (mtdnas) and the ribosomal repeat unit (ribosomal dna, rdna) of black aspergillus isolates collected in various parts of the world were examined. wide-ranging mtdna variation was observed in natural populations of the aspergillus niger aggregate. most isolates were classifiable as a. niger or aspergillus tubingensis according to their rdna and mtdna patterns. the mtdna variation was distributed unevenly in the populations studied. the mtdnas of most of the isolates collect ...19947922884
double-stranded rna mycoviruses in section nigri of the aspergillus genus.double-stranded rna bands were detected electrophoretically in about 7% of natural isolates and in 13 of 51 collection strains belonging in section nigri of the genus aspergillus. the identity of these bands was proved by s1 nuclease and rnase treatment. most of the virus-containing natural isolates came from indonesia. electron microscopic examination of the strains revealed the presence of virus-like particles in the mycelia of the strains examined. all of the virus-like particles were isometr ...19948039055
regulation of the xylanase-encoding xlna gene of aspergillus tubigensis.a gene encoding an endo-1,4-beta-xylanase from aspergillus tubigensis was cloned by oligonucleotide screening using oligonucleotides derived from amino acid sequence data obtained from the purified protein. the isolated gene was functional as it could be expressed in the very closely related fungus aspergillus niger. the xylanase encoded by this gene is synthesized as a protein of 211 amino acids. after cleavage of the presumed prepropeptide this results in a mature protein of 184 amino acids wi ...19948065265
structural features of a polygalacturonase gene cloned from aspergillus oryzae kbn616.a genomic gene encoding a polygalacturonase from aspergillus oryzae, used in soy sauce production, was cloned and sequenced. the structural gene comprises 1227 bp coding for 363 amino acids with a putative prepropeptide of 28 amino acids and the open reading frame is disrupted by two short introns of 57 bp and 81 bp. the deduced amino acid sequence of the mature protein showed 63, 63, 63 and 64% homology with those of aspergillus niger polygalacturonase i, aspergillus niger polygalacturonase ii, ...19938359678
primary structure and characterization of an exopolygalacturonase from aspergillus tubingensis.from the culture fluid of the hyphal fungus aspergillus tubingensis, an exopolygalacturonase with a molecular mass of 78 kda, an isoelectric point in the ph-range 3.7-4.4 and a ph optimum of 4.2 was purified. the enzyme has been characterized as an exopolygalacturonase [poly(1,4-alpha-d-galacturonide)galacturonohydrolase] that cleaves monomer units from the non-reducing end of the substrate molecule. k(m) and vmax for polygalacturonic acid hydrolysis were 3.2 mg ml-1 and 3.1 mg ml-1 and 255 u mg ...19968856078
arabinoxylan degradation by fungi: characterization of the arabinoxylan-arabinofuranohydrolase encoding genes from aspergillus niger and aspergillus tubingensis.the genes encoding the enzyme arabinoxylan arabinofuranohydrolase, which releases l-arabinose from arabinoxylan, have been cloned from the closely related fungi aspergillus niger and aspergillus tubingensis and were shown to be functional in a. niger. integration of multiple copies in the genome resulted in over-expression of the enzymes. the arabinofuranohydrolases encoded comprise 332 amino acids and have 94% amino acid identity. their primary structure is not related to those of other alpha-l ...19979000377
cloning of a protopectinase gene of trichosporon penicillatum and its expression in saccharomyces cerevisiae.a protopectinase (ppase)-encoding gene, pse3, from trichosporon penicillatum was cloned by colony hybridization using two oligonucleotide probes synthesized from the n-terminal amino acid sequences of native ppase se1 and one peptide from a lysyl endopeptidase digest. nucleotide sequencing revealed that pse3 contains an orf encoding a 367 amino acid protein. mature ppase se3 is composed of 340 amino acids and the n-terminus of the orf appeared to correspond to a signal peptide and a propeptide p ...19979168614
molecular cloning, primary structure, and properties of a new glycoamidase from the fungus aspergillus tubigensis.a new glycoamidase, peptide-n4-(n-acetyl-beta-d-glucosaminyl)asparagine amidase (pngase) at, was discovered in the eukaryote aspergillus tubigensis. the enzyme was purified to homogeneity, and the dna sequence was determined by cloning in escherichia coli. over 80% of the deduced amino acid sequence was verified independently by edman analysis and/or electrospray ionization-mass spectrometry of protease fragments of native pngase at. this glycoamidase contains 12 potential asparagine-linked glyc ...19979312552
the faea genes from aspergillus niger and aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides.we report the cloning and characterization of a gene encoding a ferulic acid esterase, faea, from aspergillus niger and aspergillus tubingensis. the a. niger and a. tubingensis genes have a high degree of sequence identity and contain one conserved intron. the gene product, faea, was overexpressed in wild-type a. tubingensis and a protease-deficient a. niger mutant. overexpression of both genes in wild-type a. tubingensis and an a. niger protease-deficient mutant showed that the a. tubingensis g ...19979406381
agua, the gene encoding an extracellular alpha-glucuronidase from aspergillus tubingensis, is specifically induced on xylose and not on glucuronic acid.an extracellular alpha-glucuronidase was purified and characterized from a commercial aspergillus preparation and from culture filtrate of aspergillus tubingensis. the enzyme has a molecular mass of 107 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 112 kda as determined by mass spectrometry, has a determined pi just below 5.2, and is stable at ph 6.0 for prolonged times. the ph optimum for the enzyme is between 4.5 and 6.0, and the temperature optimum is 70 d ...19989440512
targeted mutants of cochliobolus carbonum lacking the two major extracellular polygalacturonases.the filamentous fungus cochliobolus carbonum produces endo-alpha 1,4-polygalacturonase (endopg), exo-alpha 1,4-polygalacturonase (exopg), and pectin methylesterase when grown in culture on pectin. residual activity in a pgn1 mutant (lacking endopg) was due to exopg activity, and the responsible protein has now been purified. after chemical deglycosylation, the molecular mass of the purified protein decreased from greater than 60 to 45 kda. the gene that encodes exopg, pgx1, was isolated with pcr ...19989546185
overexpression of pngase at from baculovirus-infected insect cells.peptide-n4-(n-acetyl-beta-d-glucosaminyl asparagine amidase) from aspergillus tubigensis (pngase at) was expressed in baculovirus-infected insect cells. the recombinant pngase at was secreted and purified to homogeneity with a yield of 9.5 mg per liter of infected cell medium. recombinant pngase at migrated upon sds-page as a single-chain protein with a molecular mass of 78 kda. this contrasts with the native aspergillus enzyme which is "nicked" and migrates as two subunits each with a molecular ...19989790895
the exopolygalacturonase from aspergillus tubingensis is also active on xylogalacturonan.apple-pectin hairy regions were prepared from apple pectin by combined action of the recombinant aspergillus niger enzymes endopolygalacturonase ii and pectin methylesterase and the a. tubigensis exopolygalacturonase. using this enzymically prepared pectin fraction, an additional activity of the a. tubigensis exopolygalacturonase was discovered only when the substrate was chemically saponified and when d-galacturonate, a potent inhibitor of the enzyme, was removed from the incubation mixture. th ...199910467119
new pcr method to differentiate species in the aspergillus niger aggregate.the dna that encodes the 5.8s gene of the ribosomal rna and the two intergenic spacers its1 and its2 of the two proposed type strains of the aspergillus niger aggregate (a. niger and aspergillus tubingensis) have been sequenced. by comparison of sequences we have found that both species could be differentiated by rsai digestion of the pcr products of the mentioned regions. this method could be a useful tool in the identification of strains of the a. niger aggregate, especially in studies that in ...199910556711
performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates.two monomethyl esters of alpha-(1-4)-linked d-galacturonic dimers and three monomethyl esters of alpha-(1-4)-linked d-galacturonic acid trimers were synthesized chemically and further used as substrates in order to establish the substrate specificity of six different endopolygalacturonases from aspergillus niger, one exopolygalacturonase from aspergillus tubingensis, and four selected erwinia chrysanthemi pectinases; exopolygalacturonan hydrolase x (pehx), exopolygalacturonate lyase x (pelx), ex ...199910601263
endo-xylogalacturonan hydrolase, a novel pectinolytic enzyme.we screened an aspergillus tubingensis expression library constructed in the yeast kluyveromyces lactis for xylogalacturonan-hydrolyzing activity in microwell plates by using a bicinchoninic acid assay. this assay detects reducing carbohydrate groups when they are released from a carbohydrate by enzymatic activity. two k. lactis recombinants exhibiting xylogalacturonan-hydrolyzing activity were found among the 3,400 colonies tested. the cdna insert of these recombinants encoded a 406-amino-acid ...200010618200
cloning and disruption of pgx4 encoding an in planta expressed exopolygalacturonase from fusarium oxysporum.fusarium oxysporum f. sp. lycopersici, the causal agent of tomato vascular wilt, produces an array of pectinolytic enzymes, including at least two exo-alpha1,4-polygalacturonases (exopgs). a gene encoding an exopg, pgx4, was isolated with degenerate polymerase chain reaction primers derived from amino acid sequences conserved in two fungal exopgs. pgx4 encodes a 454 amino acid polypeptide with nine potential n-glycosylation sites and a putative 21 amino acid n-terminal signal peptide. the deduce ...200010755298
inverting character of alpha-glucuronidase a from aspergillus tubingensis.alpha-glucuronidase a from aspergillus tubingensis was found to be capable of liberating 4-o-methyl-d-glucuronic acid (meglca) only from those beechwood glucuronoxylan fragments in which the acid is attached to the non-reducing terminal xylopyranosyl residue. reduced aldotetrauronic acid, 4-o-methyl-d-glucuronosyl-alpha-1,2-d-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylitol, was found to be a suitable substrate to follow the stereochemical course of the hydrolytic reaction catalyzed by the ...200010779688
dihydrocarbazole alkaloids from aspergillus tubingensis.investigation of the fungus aspergillus tubingensis has led to the isolation and identification of two dihydrocarbazole-containing compounds (1 and 2). details of the purification and structure elucidation of 1 and 2 are described. this is the first known report of dihydrocarbazole-containing compounds to be isolated from a living system.200111421760
biosolubilization of poorly soluble rock phosphates by aspergillus tubingensis and aspergillus niger.three isolates of aspergillus tubingensis and two isolates of aspergillus niger isolated from rhizospheric soils were tested on solubilization of different rock phosphates. all the isolates of aspergillus were capable of solubilizing all the natural rock phosphates. a. tubingensis (at1) showed maximum percent solubilization in all the rock phosphates tested in this study when compared to other isolates. this isolate also showed highest phosphorus (p) solubilization when grown in the presence of ...200212139336
curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant.a presumed antimicrobial enzyme system, the curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. in the presence of hydrogen peroxide, curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. the curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (pseudomonas spp., escherichia coli, serratia marcescens, aeromonas salmonicida, s ...200312902249
mitochondrial dna organisation of the mtdna type 2b of aspergillus tubingensis compared to the aspergillus niger mtdna type 1a.a physical and functional map of aspergillus tubingensis mtdna type 2b was constructed and compared to aspergillus niger typei a mtdna. the gene content and order, as well as the patterns of restriction sites, were similar. two unidentified orfs and several repeat elements were found in the region between the ndhl and ndh4 genes on both mtdnas. the sizes of the a. niger and a. tubingensis mtdnas were 31.23 and 33.06 kb. respectively, the difference was principally attributed to the altered intro ...200415600010
aspergillus vadensis, a new species of the group of black aspergilli.a strain from the group of black aspergilli was analysed in detail to determine the species to which it belongs. a detailed analysis of morphology, rflp patterns and metabolite profiles was carried out. in addition, a phylogenetic tree was constructed for the black aspergilli using the its and the beta-tubulin sequences of the individual strains. the new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone b, nigragillin, asp ...200515803385
study of spanish grape mycobiota and ochratoxin a production by isolates of aspergillus tubingensis and other members of aspergillus section nigri.the native mycobiota of five grape varieties grown in spain has been studied. four (bobal, tempranillo, garnacha, and monastrell) were red varieties and one (moscatel) was white. the main fungal genera isolated were alternaria, cladosporium, and aspergillus. the isolation frequency of aspergillus spp. section nigri in contaminated samples was 82%. ochratoxin a (ota) production was assessed using yeast extract-sucrose broth supplemented with 5% bee pollen. cultures of 205 isolates from this secti ...200516085865
cloning, sequencing and expression of an alpha-l-arabinofuranosidase from aspergillus sojae.the arabinofuranosidase gene was cloned from the cdna of aspergillus sojae. it was found to contain an open reading frame composed of 984 base pairs (bp) and to encode 328 amino acid residues (aa). the cdna sequence suggested that the mature enzyme is preceded by a 26-aa signal sequence and the molecular mass was predicted to be 32,749 da. the a. sojae arabinofuranosidase consists of a single catalytic domain; it does not have a specific substrate-binding domain such as the xylan-binding domain ...200016232740
isolation and sequencing of a new glucoamylase gene from an aspergillus niger aggregate strain (dsm 823) molecularly classified as aspergillus tubingensis.based on morphological characteristics the taxa included in the aspergillus aggregate can hardly be differentiated. for that reason the phylogeny of this genus was revised several times as different criteria, from morphological to later molecular, were used. we found, comparing nucleotide sequences of the its-region, that the strain aspergillus niger (dsm 823) which is claimed to be identical to the strains atcc 10577, imi 027809, nctc 7193 and nrrl 2322 can be molecularly classified as aspergil ...200516284933
ochratoxin a production and amplified fragment length polymorphism analysis of aspergillus carbonarius, aspergillus tubingensis, and aspergillus niger strains isolated from grapes in italy.ochratoxin a is a potent nephrotoxin and a possible human carcinogen that can contaminate various agricultural products, including grapes and wine. the capabilities of species other than aspergillus carbonarius within aspergillus section nigri to produce ochratoxin a from grapes are uncertain, since strain identification is based primarily on morphological traits. we used amplified fragment length polymorphisms (aflps) and genomic dna sequences (rrna, calmodulin, and beta-tubulin genes) to ident ...200616391107
aflp characterization of southern europe population of aspergillus section nigri from grapes.members of aspergillus belonging to section nigri are distributed worldwide and are mainly responsible for the ochratoxin a accumulation in grapes and wine, particularly in southern europe. limited information is available on the species composition and genetic variability of black aspergilli strains occurring on grapes. we analyzed 283 representative strains from the main wine producing european countries collected in 2001-2002 (italy, france, spain, portugal, greece and israel) using amplified ...200616682091
an its-rflp method to identify black aspergillus isolates responsible for ota contamination in grapes and wine.ochratoxigenic mycobiota in grapes from representative wine regions in valencia was identified. black aspergilli were predominant among the different aspergillus spp. isolated. restriction digestion analysis of the its products was tested as a rapid method to identify isolates of black aspergillus species from grapes. restriction endonuclease digestion of the its products using the endonucleases hhai, nlaiii and rsai, distinguished five types of restriction fragment length polymorphism (rflp) co ...200716870292
asperpyrone d and other metabolites of the plant-associated fungal strain aspergillus tubingensis.bioactivity-guided fractionation of a cytotoxic extract of aspergillus tubingensis, a fungal strain occurring in the rhizosphere of the sonoran desert plant, fallugia paradoxa, afforded a dimeric naphtho-gamma-pyrone asperpyrone d, nine known naphtho-gamma-pyrones, funalenone, and the cytotoxic cyclic penta-peptide, malformin a1.200717150233
aspergillus brasiliensis sp. nov., a biseriate black aspergillus species with world-wide distribution.a novel species, aspergillus brasiliensis sp. nov., is described within aspergillus section nigri. this species can be distinguished from other black aspergilli based on intergenic transcribed region, beta-tubulin and calmodulin gene sequences, by amplified fragment length polymorphism analysis and by extrolite profiles. a. brasiliensis isolates produced naphtho-gamma-pyrones, tensidol a and b and pyrophen in common with aspergillus niger and aspergillus tubingensis, but also several unique comp ...200717684283
polymerase chain reaction (pcr) identification of aspergillus niger and aspergillus tubingensis based on the calmodulin gene.aspergillus niger and a. tubingensis, species belonging to section nigri, are commonly found in plant products and processed food, such as grapes, cereals, coffee, and derived products. these two species are very difficult to differentiate by classical morphological criteria and some isolates are known to produce ochratoxin a. the exact identification of these two species is very important to avoid the overestimation of toxicological contamination and related risks. a polymerase chain reaction ( ...200717886188
inhibition of species of the aspergillus section nigri and ochratoxin a production in grapes by fusapyrone.fusapyrone (fp), an antifungal natural compound, was tested against the three main ochratoxigenic species of the aspergillus section nigri. the mics at 24 h were 6.0, 11.6, and 9.9 mug/ml for aspergillus carbonarius, aspergillus tubingensis, and aspergillus niger, respectively. strong inhibition of growth and morphological changes were still observed at half the mic after 7 days. the application of a 100 mug/ml fp solution in a laboratory assay on artificially inoculated grapes resulted in a sig ...200818263739
comparative analysis of the complete mitochondrial genomes of aspergillus niger mtdna type 1a and aspergillus tubingensis mtdna type 2b.to understand the differences in the organization of mitochondrial genomes of the very closely related aspergillus niger and aspergillus tubingensis species, we determined the complete genome sequence of the 1a mtdna type of a. niger and 2b mtdna type of a. tubingensis and now we provide a comparative analysis of the two mtdnas. we found that (1) the organization (gene content and order) of the two genomes is almost identical and (2) the size difference between them is principally attributed to ...200818318841
effect of carbon and nitrogen sources on phosphate solubilization by a wild-type strain and uv-induced mutants of aspergillus tubingensis.the mechanisms of action of phosphate solubilization were studied in the wild-type strain aspergillus tubingensis and the phenotypic mutants derived from it. the p solubilization activities of these isolates were measured in liquid media using different carbon and nitrogen sources. all the mutants showed higher p solubilization compared to the wild type. glucose and sucrose significantly promoted p solubilization compared to fructose, lactose, galactose, and xylose. potassium nitrate significant ...200818663523
cr(vi) reduction by an aspergillus tubingensis strain: role of carboxylic acids and implications for natural attenuation and biotreatment of cr(vi) contamination.experiments conducted in shake flask cultures, in minimal medium of ph 5.3 containing 50 microg ml(-1)cr(vi) with glucose as a carbon source, indicated that the biomass of aspergillus sp. strain ed8, a chromate-tolerant fungal strain previously isolated from a chromium-polluted soil, responds to the presence of citrate in the medium by increasing the rate of cr(vi) reduction; this effect required the use of live biomass and was not observed in medium with lactate. other natural carboxylic acids ...200919286242
infectious keratitis caused by aspergillus tubingensis.to report 2 cases of keratomycosis caused by aspergillus tubingensis.200919654512
impact of some environmental factors on growth and production of ochratoxin a of/by aspergillus tubingensis, a. niger, and a. carbonarius isolated from moroccan grapes.the effects of temperature, water activity (aw), incubation time, and their combinations on radial growth and ochratoxin a (ota) production of/by eight aspergillus niger aggregate strains (six a. tubingensis and two a. niger) and four a. carbonarius isolated from moroccan grapes were studied. optimal conditions for the growth of most studied strains were shown to be at 25 degrees c and 0.95 aw. no growth was observed at 10 degrees c regardless of the water activity and isolates. the optimal temp ...200919763414
isolation and identification of two new fungal strains for xylanase production.fungi are well known for their ability to excrete enzymes into the environment. the aim of this work was to evaluate xylanase production by fungi isolated from soil. one hundred and thirty-six fungal isolates were screened for xylanase production. two xylanase producing isolates, fss117 and fss129, were identified on the basis of analyses of 5,8s gene sequencing. the closest phylogenetic neighbors according to 5,8s gene sequence data for the two isolates were aspergillus tubingensis and aspergil ...201020383603
identification and characterisation of human pathogenic filamentous fungi and susceptibility to thymus schimperi essential oil.summary twenty-eight clinical fungal isolates were characterised by morphological (macro- and micro-features and growth response at 25, 30 and 37 degrees c) and molecular (nuclear rdna-internal transcriber spacer, calmodulin, cytochrome c oxidase 1 and the largest subunit of rna polymerase ii) analyses. the clinical fungal isolates were ascribed to the following taxa: penicillium chrysogenum, verticillium sp., aspergillus tubingensis, aspergillus minutus, beauveria bassiana and microsporum gypse ...201020633233
Genetic re-identification and antifungal susceptibility testing of Aspergillus section Nigri strains of the BCCM/IHEM collection.Black Aspergilli are widely distributed in the environment and are frequently reported as causative agents of different types of mycoses. Many taxonomical revisions have been made, and presently 19 different species are accepted. In this study we (re-) identified 123 strains of the Aspergillus niger group of the BCCM/IHEM collection to check for the presence of species other than A. niger in both environmental and clinical samples. The susceptibility for antifungal drugs was compared between A. ...201121929560
Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran.Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carri ...201121895787
Aspergillus niger contains the cryptic phylogenetic species A. awamori.Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger 'aggregate' represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compar ...201122036292
production of xylanase under solid-state fermentation by aspergillus tubingensis jp-1 and its application.the production of extracellular xylanase by a locally isolated strain of aspergillus tubingensis jp-1 was studied under solid-state fermentation. among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. the influence of various parameters such as initial ph, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. the production of the xylanase reached a peak ...201222271252
season and tissue type affect fungal endophyte communities of the indian medicinal plant tinospora cordifolia more strongly than geographic location.a total of 1,151 endophytic fungal isolates representing 29 taxa were isolated from symptom-less, surface-sterilized segments of stem, leaf, petiole, and root of tinospora cordifolia which had been collected at three locations differing in air pollution in india (ramnagar, banaras hindu university, maruadih) during three seasons (summer, monsoon, winter). endophytes were most abundant in leaf tissues (29.38% of all isolates), followed by stem (18.16%), petiole (10.11%), and root segments (6.27%) ...201222430503
identification and mycotoxigenic capacity of fungi associated with pre- and postharvest fruit rots of pomegranates in greece and cyprus.pre- and postharvest fruit rots of fungal origin are an important burden for the pomegranate industry worldwide, affecting the produce both quantitatively and qualitatively. during 2013, local orchards were surveyed and 280 fungal isolates from greece (gr) and cyprus (cy) were collected from pomegranates exhibiting preharvest rot symptoms, and additional 153 isolates were collected postharvest from cold-stored fruit in gr. molecular identification revealed that preharvest pomegranate fruit rots ...201526057112
species distribution and in vitro azole susceptibility of aspergillus section nigri isolates from clinical and environmental settings.aspergillus section nigri includes species of interest for animal and human health, although studies on species distribution are limited to human cases. data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. forty-two black aspergillus strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their in vitro triazole susceptibilities inve ...201627413191
aspergillus tubingensis and aspergillus niger as the dominant black aspergillus, use of simple pcr-rflp for preliminary differentiation.this work aimed to identify the species distribution of common clinical and environmental isolates of black aspergilli based on simple restriction fragment length polymorphism (rflp) analysis of the β-tubulin gene. a total of 149 clinical and environmental strains of black aspergilli were collected and subjected to preliminary morphological examination. total genomic dnas were extracted, and pcr was performed to amplify part of the β-tubulin gene. at first, 52 randomly selected samples were spec ...201626852194
comparison of species composition and fumonisin production in aspergillus section nigri populations in maize kernels from usa and italy.fumonisin contamination of maize is considered a serious problem in most maize-growing regions of the world, due to the widespread occurrence of these mycotoxins and their association with toxicosis in livestock and humans. fumonisins are produced primarily by species of fusarium that are common in maize grain, but also by some species of aspergillus sect. nigri, which can also occur on maize kernels as opportunistic pathogens. understanding the origin of fumonisin contamination in maize is a ke ...201425087207
taxonomic re-evaluation of black koji molds.black koji molds including its albino mutant, the white koji mold, have been widely used for making the distilled spirit shochu in northeast asia because they produce citric acid which prevents undesirable contamination from bacteria. since inui reported aspergillus luchuensis from black koji in okinawa in 1901, many fungal names associated with black koji molds were reported. however, some species are similar and differentiation between species is difficult. fungal taxonomists tried to arrange ...201424281756
distribution and mycotoxigenic potential of aspergillus section nigri species in naturally contaminated almonds.in a previous study, inedible almond pick-out samples were assayed for aflatoxin and aflatoxigenic aspergillus species. these samples contained high populations of black-spored aspergillus section nigri species. to investigate whether these species may contribute to the total potential mycotoxin content of almonds, aspergillus section nigri strains were isolated from these samples and assayed for ochratoxin a (ota) and fumonisin b2 (fb2). the majority of isolates (117 strains, 68%) were identifi ...201323575138
multi-resistant aspergillosis due to cryptic species.reports of cryptic species causing aspergillosis in humans are increasing in the literature. cryptic species are defined as those which are morphologically indistinguishable, although their identifications can be confirmed using molecular or other techniques which continue to become more widely available in the clinical setting. antifungal resistance has often been noted in these cases, and indeed there does appear to be a higher prevalence of reduced susceptibility in cryptic species. many of t ...201424969615
aspergillus species as emerging causative agents of onychomycosis.onychomycosis is a common nail infection caused by dermatophytes, non-dermatophyte molds (ndm), and yeasts. aspergillus species are emerging as increasing causes of toenail onychomycosis. the purpose of this study was species delineation of aspergillus spp. isolated from patients with onychomycosis.201525656408
characterization of aspergillus section nigri species populations in vineyard soil using droplet digital pcr.identification of populations of aspergillus section nigri species in environmental samples using traditional methods is laborious and impractical for large numbers of samples. we developed species-specific primers and probes for quantitative droplet digital pcr (ddpcr) to improve sample throughput and simultaneously detect multiple species in each sample. the ddpcr method was used to distinguish aspergillus niger, aspergillus welwitschiae, aspergillus tubingensis and aspergillus carbonarius in ...201627604045
diversity of black aspergilli isolated from raisins in argentina: polyphasic approach to species identification and development of scar markers for aspergillus ibericus.aspergillus section nigri is a heterogeneous fungal group including some ochratoxin a producer species that usually contaminate raisins. the section contains the series carbonaria which includes the toxigenic species aspergillus carbonarius and nontoxigenic aspergillus ibericus that are phenotypically undistinguishable. the aim of this study was to examine the diversity of black aspergilli isolated from raisins and to develop a specific genetic marker to distinguish a. ibericus from a. carbonari ...201526114593
detection and discrimination of four aspergillus section nigri species by pcr.species of aspergillus section nigri are not easily distinguished by traditional morphological techniques, and typically are identified by dna sequencing methods. we developed four pcr primers to distinguish between aspergillus niger, aspergillus welwitschiae, aspergillus carbonarius and aspergillus tubingensis, based on species-conserved differences in the calmodulin gene sequence. pcr amplification from total dna using these primers was species specific; no amplification occurred from nontarge ...201525384730
analyses of black aspergillus species of peanut and maize for ochratoxins and fumonisins.the genus aspergillus section nigri, or the black aspergilli, represents genetically closely related species that produce the mycotoxins, ochratoxins and the fumonisins. fumonisin b1 (fb1) is of an added concern because it is also a virulence factor for maize. our preliminary data indicated that black aspergilli could develop asymptomatic infections with maize and peanuts plants. symptomless infections are potential problems, because under favorable conditions, there is a potential for accumulat ...201424780336
ecophysiological characterization of aspergillus carbonarius, aspergillus tubingensis and aspergillus niger isolated from grapes in spanish vineyards.the aim of this study was to evaluate the diversity of black aspergilli isolated from berries from different agroclimatic regions of spain. growth characterization (in terms of temperature and water activity requirements) of aspergillus carbonarius, aspergillus tubingensis and aspergillus niger was carried out on synthetic grape medium. a. tubingensis and a. niger showed higher maximum temperatures for growth (>45 °c versus 40-42 °c), and lower minimum aw requirements (0.83 aw versus 0.87 aw) th ...201424412963
biodiversity of aspergillus section nigri populations in argentinian vineyards and ochratoxin a contamination.aspergillus section nigri are described as the main source of ochratoxin a (ota) contamination in grapes and wine worldwide. the knowledge of the factors affecting grape contamination by species included in this section and ota production is essential to be able to reduce their presence, not only to improve wine quality, but also to maintain their safety. therefore, the aims of this study were to determine the incidence of aspergillus section nigri species harvested in different grape-growing re ...201324010597
genetic diversity of aspergillus species isolated from onychomycosis and aspergillus hongkongensis sp. nov., with implications to antifungal susceptibility testing.thirteen aspergillus isolates recovered from nails of 13 patients (fingernails, n=2; toenails, n=11) with onychomycosis were characterized. twelve strains were identified by multilocus sequencing as aspergillus spp. (aspergillus sydowii [n=4], aspergillus welwitschiae [n=3], aspergillus terreus [n=2], aspergillus flavus [n=1], aspergillus tubingensis [n=1], and aspergillus unguis [n=1]). isolates of a. terreus, a. flavus, and a. unguis were also identifiable by matrix-assisted laser desorption/i ...201626658315
characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents.a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ...201525832181
sexual reproduction in aspergillus tubingensis from section nigri.a sclerotium-forming member of aspergillus section nigri was sampled from a population in a single field in north carolina, usa, and identified as a. tubingensis based on genealogical concordance analysis. aspergillus tubingensis was shown to be heterothallic, with individual strains containing either a mat1-1 or mat1-2 mating-type gene. strains of opposite mating type were crossed on mixed cereal agar and incubated for 5-6 months. stromata typically formed 1-2 indehiscent ascocarps containing a ...201323709489
characterization of thermophilic fungal community associated with pile fermentation of pu-erh tea.this study aimed to characterize the thermophilic fungi in pile-fermentation process of pu-erh tea. physicochemical analyses showed that the high temperature and low ph provided optimal conditions for propagation of fungi. a number of fungi, including blastobotrys adeninivorans, thermomyces lanuginosus, rasamsonia emersonii, aspergillus fumigatus, rhizomucor pusillus, rasamsonia byssochlamydoides, rasamsonia cylindrospora, aspergillus tubingensis, aspergillus niger, candida tropicalis and fusari ...201627046629
fungal isolates from a pu-erh type tea fermentation and their ability to convert tea polyphenols to theabrownins.the natural microbiota involved in the fermentation influence the quality and taste of fully postfermented teas such as china's pu-erh tea. ten microbial isolates representing 6 species were recovered from a solid-state fermentation of a pu-erh type tea. the isolates were aspergillus tubingensis, aspergillus marvanovae, rhizomucor pusillus, rhizomucor tauricus, aspergillus fumigatus, and candida mogii. with the exception of a. marvanovae and c. mogii, all these microorganisms have been previousl ...201525799937
what causes canine sino-nasal aspergillosis? a molecular approach to species identification.on the basis of phenotypic identification methods, aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (sna). however, definitive identification of aspergillus spp. using phenotypic features alone is unreliable. the aim of this study was to determine the molecular identities of fungal species causing sna in dogs. genomic dna was extracted from 91 fungal isolates from 90 dogs diagnosed with sna in australia, the usa and belgium, ...201424559745
analysis of the mat1-1 and mat1-2 gene ratio in black koji molds isolated from meju.aspergillus luchuensis is known as an industrially important fungal species used for making fermented foods such as awamori and shochu in japan, makgeolli and meju in korea, and pu-erh tea in china. nonetheless, this species has not yet been widely studied regarding mating-type genes. in this study, we examined the mat1-1 and mat1-2 gene ratio in black koji molds (a. luchuensis, aspergillus niger, and aspergillus tubingensis) and in aspergillus welwitschiae isolated from meju, a fermented soybea ...201628154484
design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.microbial interactions are important for metabolism as they can improve or reduce metabolic efficiency. to improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including trichoderma reesei, penicillium decumbens, aspergillus tubingensis, and aspergillus niger. however, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strain ...201728039824
effect of fungal mycelia on the hplc-uv and uv-vis spectrophotometric assessment of mycelium-bound epoxide hydrolase using glycidyl phenyl ether.the use of mycelia as biocatalysts has technical and economic advantages. however, there are several difficulties in obtaining accurate results in mycelium-catalysed reactions. firstly, sample extraction, indispensable because of the presence of mycelia, can bring into the extract components with a similar structure to that of the analyte of interest; secondly, mycelia can influence the recovery of the analyte. we prepared calibration standards of 3-phenoxy-1,2-propanediol (ppd) in the pure solv ...201626902669
triazole susceptibilities in thermotolerant fungal isolates from outdoor air in the seoul capital area in south korea.emerging fungi resistant to triazoles are a concern because of the increased use of medical triazoles and exposure to agricultural triazoles. however, little is known about the levels of triazole susceptibility in outdoor airborne fungi making it difficult to assess the risks of inhalation exposure to airborne, antifungal-resistant fungi. this study examined triazole susceptibilities of the airborne thermotolerant fungi isolated from the ambient air of the seoul capital area of south korea. we u ...201526405807
sexual recombination in aspergillus tubingensis.aspergillus tubingensis from section nigri (black aspergilli) is closely related to a. niger and is used extensively in the industrial production of enzymes and organic acids. we recently discovered sexual reproduction in a. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. progeny were obtained from six crosses involving five mat1-1 strains and two mat1-2 strains. we examined three loci, including mating type (mat), rna polymerase ii (rpb2) and β-tubulin (b ...201525572097
identification and susceptibility of aspergillus section nigri in china: prevalence of species and paradoxical growth in response to echinocandins.molecular identification and in vitro antifungal susceptibility tests of 43 aspergillus section nigri isolates from china were performed. aspergillus niger and aspergillus tubingensis were present in almost equal numbers. all of the isolates had low mic/mecs (minimum effective concentrations) for the 7 common antifungals, and a paradoxical effect was observed for the first time in response to caspofungin and micafungin.201525502526
overexpression of aspergillus tubingensis faea in protease-deficient aspergillus niger enables ferulic acid production from plant material.the production of ferulic acid esterase involved in the release of ferulic acid side groups from xylan was investigated in strains of aspergillus tubingensis, aspergillus carneus, aspergillus niger and rhizopus oryzae. the highest activity on triticale bran as sole carbon source was observed with the a. tubingensis t8.4 strain, which produced a type a ferulic acid esterase active against methyl p-coumarate, methyl ferulate and methyl sinapate. the activity of the a. tubingensis ferulic acid este ...201424664515
involvement of the opportunistic pathogen aspergillus tubingensis in osteomyelitis of the maxillary bone: a case report.aspergillus tubingensis is a black aspergillus belonging to the aspergillus section nigri, which includes species that morphologically resemble aspergillus niger. recent developments in species determination have resulted in clinical isolates presumed to be aspergillus niger being reclassified as aspergillus tubingensis by sequencing. we present a report of a patient with an osteomyelitis of the maxillary bone with a probable invasive aspergillus tubingensis infection.201323374883
microbial dynamics of commercial makgeolli depending on the storage temperature.market fresh makgeolli was stored at different temperatures of 4°c and 25°c to assess the change of the microbial diversity according to the storage temperature and period. yeast counts increased until day 3 of storage and decreased thereafter. general and lactic acid bacterial counts continuously increased during storage. the data indicated that the control of growth of microorganisms, particularly general bacteria and lactic acid bacteria (lab), is essential. total acid levels started to decre ...201222713986
decolorization and semi-batch continuous treatment of molasses distillery wastewater by aspergillus tubingensis dct6.large quantities of deeply pigmented molasses distillery wastewater (mdw), are discharged during the production of bio-ethanol from molasses. conventional biological wastewater treatment is not effective in removing the molasses pigments. in the present study, a mdw treatment system was developed with combination treatment involving biodecolorization and biotreatment by aspergillus tubingensis dct6, together with physical decolorization by ozonation after treatment by activated sludge. a. tubing ...200919494457
interpretation of mtdna rflp variability among aspergillus tubingensis isolates.aspergillus tubingensis isolates collected from distant geographic areas were earlier classified into six groups on the basis of the mtdna rflp variability they exhibited (mtdna types 2a-2f). in the present work, we investigated the reason for the intraspecific mtdna variability and we describe here how this fungus, with a relatively small mitochondrial genome, can display intraspecific polymorphism due to intron acquisition and also sporadic point mutations affecting the recognition motifs of t ...200717043909
characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium thermotoga maritima.an intracellular pectinolytic enzyme, pelb (tm0437), from the hyperthermophilic bacterium thermotoga maritima was functionally produced in escherichia coli and purified to homogeneity. pelb belongs to family 28 of the glycoside hydrolases, consisting of pectin-hydrolysing enzymes. as one of the few bacterial exopolygalacturonases, it is able to remove monogalacturonate units from the nonreducing end of polygalacturonate. detailed characterization of the enzyme showed that pelb is highly thermo-a ...200516262687
identification, classification and phylogeny of the aspergillus section nigri inferred from mitochondrial cytochrome b gene.the partial mitochondrial cytochrome b gene from 32 strains of 12 species belonging to aspergillus section nigri was amplified by the polymerase chain reaction and sequenced directly. using 402 nucleotide characters, nucleotide-based and amino acid-based phylogenetic trees were inferred and the genetic divergence among the species was evaluated. based on analyses of the 402-bp nucleotide and 133-amino acid sequences, strains were divided into 11 dna types and five amino acid types. aspergillus n ...200111425482
beta-glucosidase multiplicity from aspergillus tubingensis cbs 643.92: purification and characterization of four beta-glucosidases and their differentiation with respect to substrate specificity, glucose inhibition and acid tolerance.from aspergillus tubingensis cbs 643.92 four distinct beta-glucosidases (i-iv) were purified by a four-step purification procedure. sds-page revealed molecular masses of 131, 126, 54 and 54 kda, respectively, and their isoelectric points were determined to be 4.2, 3.9, 3.7 and 3.6, respectively. the beta-glucosidases exhibited high diversity with respect to ph and temperature optima and stability, as well as to substrate specificity and glucose tolerance. the major beta-glucosidase (i) preferent ...200111330708
molecular characterization and antimicrobial activity of an endolichenic fungus, aspergillus sp. isolated from parmelia caperata of similipal biosphere reserve, india.endolichenic fungi are microbes that inhabit healthy inner lichen tissues without any disease symptoms. they have been reported to produce new and interesting bioactive metabolites. in the present study, an endolichenic fungus frequently isolated from surface-sterilized lichen thallus of parmelia caperata has been described. the fungus was identified as aspergillus tubingensis based on morphological traits and its rdna sequence. crude metabolites extracted from the culture broth exhibited consid ...201626792125
aspergillus tubingensis: a major filamentous fungus found in the airways of patients with lung disease.the black aspergillus group comprises a. niger and 18 other species, which are morphologically indistinguishable. among this species subset, a. tubingensis, described in less than 30 human cases before 2014, is primarily isolated from ear, nose, and throat samples. recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has emerged as a powerful technique to identify microbes in diagnostic settings. we applied this method to identify 1,720 filamentous fungi routine ...201626773134
a new pyrone derivative from an endophytic aspergillus tubingensis of lycium ruthenicum.a new pyrone named 6-isovaleryl-4-methoxy-pyran-2-one (1), along with three known pyrone compounds, rubrofusarin b (2), asperpyrones a (3) and campyrone a (4), was isolated from fermentation of aspergillus tubingensis in lycium ruthenicum. their structures were confirmed by spectroscopic techniques, such as ir, nmr and hresi-ms. compound 2 indicated strong inhibitory activity against escherichia coli, with mic value of 1.95 μg/ml.201626610038
fungal diversity in grape must and wine fermentation assessed by massive sequencing, quantitative pcr and dgge.the diversity of fungi in grape must and during wine fermentation was investigated in this study by culture-dependent and culture-independent techniques. carignan and grenache grapes were harvested from three vineyards in the priorat region (spain) in 2012, and nine samples were selected from the grape must after crushing and during wine fermentation. from culture-dependent techniques, 362 isolates were randomly selected and identified by 5.8s-its-rflp and 26s-d1/d2 sequencing. meanwhile, genomi ...201526557110
biocontrol of tomato plant diseases caused by fusarium solani using a new isolated aspergillus tubingensis ctm 507 glucose oxidase.the present study focuses on the potential of glucose oxidase (god) as a promising biocontrol agent for fungal plant pathogens. in fact, a new god producing fungus was isolated and identified as an aspergillus tubingensis. god (125 au) has been found to inhibit fusarium solani growth and spore production. indeed, god caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. in vivo assays, god acted as a curative treatment ...201526299190
purification, biochemical characterization and antifungal activity of a novel aspergillus tubingensis glucose oxidase steady on broad range of ph and temperatures.this study was carried out to evaluate the in vitro and in vivo antifungal efficiency of aspergillus tubingensis ctm 507 glucose oxidase (god) against plant pathogenic fungi. god displayed a wide inhibitory spectrum toward different fungi at a concentration of 20 au. the god had a strong inhibitor effect on mycelia growth and spore germination of pythium ultimum. interestingly, the god exhibited a potent in vivo antifungal effect against p. ultimum responsible for potato plants disease. the anti ...201526280215
anti-tmv activity of malformin a1, a cyclic penta-peptide produced by an endophytic fungus aspergillus tubingensis fjbj11.plant-associated microorganisms are known to produce a variety of metabolites with novel structures and interesting biological activities. an endophytic fungus fjbj11, isolated from the plant tissue of brucea javanica (l.) merr. (simaroubaceae), was proven to be significantly effective in producing metabolites with anti-tobacco mosaic virus (tmv) activities. the isolate was identified as aspergillus tubingensis fjbj11 based on morphological characteristics and its sequence. bioassay-guided isola ...201525775156
kinetic and thermodynamic characterization of a halotolerant β-galactosidase produced by halotolerant aspergillus tubingensis gr1.β-galactosidase from halotolerant aspergillus tubingensis gr1 was purified by two-step purification process comprising ammonium sulfate precipitation followed by size exclusion chromatography (sec). the recovery of β-galactosidase after sec was found to be 1.40% with 58.55-fold increase in specific activity. the molecular weight of β-galactosidase protein was found to be 93 kda by sds-page. activation energy for o-nitrophenol β-d-galactopyranoside (onpg) hydrolysis was 32.88 kj mol(-1), while te ...201525656109
biodegradation of ochratoxin a by aspergillus tubingensis isolated from meju.ochratoxin a (ota), a mycotoxin, contaminates agricultural products and poses a serious threat to public health worldwide. microbiological methods are known to be a promising approach for ota biodegradation because physical and chemical methods have practical limitations. in the present study, a total of 130 fungal isolates obtained from 65 traditional korean meju (a fermented starter for fermentation of soybeans) samples were examined for ota-biodegradation activity using thin-layer chromatogra ...201627558439
influence of temperature, water activity and incubation time on fungal growth and production of ochratoxin a and zearalenone by toxigenic aspergillus tubingensis and fusarium incarnatum isolates in sorghum seeds.the major objective of this study was to describe the effect of water activity and temperature on radial growth and production of ochratoxin a (ota) and zearalenone (zea) on sorghum grains of three aspergillus tubingensis and three fusarium incarnatum isolates. the water activity range was 0.91-0.99 aw for f. incarnatum isolates and 0.88-0.99 aw for a. tubingensis isolates. temperatures of incubation were 15, 25 and 37°c for both species. mycotoxin production was determined after 7, 14, 21 and 2 ...201727883966
sensitivity of neurospora crassa to a marine-derived aspergillus tubingensis anhydride exhibiting antifungal activity that is mediated by the mas1 protein.the fungus aspergillus tubingensis (strain oy907) was isolated from the mediterranean marine sponge ircinia variabilis. extracellular extracts produced by this strain were found to inhibit the growth of several fungi. among the secreted extract components, a novel anhydride metabolite, tubingenoic anhydride a (1) as well as the known 2-carboxymethyl-3-hexylmaleic acid anhydride, asperic acid, and campyrone a and c were purified and their structure elucidated. compound 1 and 2-carboxymethyl-3-hex ...201425257783
mechanisms of interaction of chromium with aspergillus niger var tubingensis strain ed8.experiments were conducted to determine the mechanisms of interaction with chromium of aspergillus niger var tubingensis strain ed8 in batch culture and in bioreactor experiments. results obtained in this work showed that the interaction of a. niger var tubingensis ed8 with cr(vi) is based mainly in a reduction process and also, secondly, in a sorption process. using electron microscopy techniques the ultrathin sections obtained from the mycelium biomass produced by the fungus in batch cultures ...201424607453
conversion of fusaric acid to fusarinol by aspergillus tubingensis: a detoxification reaction.the fungus fusarium oxysporum causes wilt diseases of plants and produces a potent phytotoxin fusaric acid (fa), which is also toxic to many microorganisms. an aspergillus tubingensis strain with high tolerance to fa was isolated from soil and designated as cdrat01. hplc analysis of culture filtrates from a. tubingensis isolate cdrat01 grown with the addition of fa indicated the formation of a metabolite over time that was associated with a decrease of fa. spectral analysis and chemical synthesi ...201424352475
raw starch conversion by saccharomyces cerevisiae expressing aspergillus tubingensis amylases.starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. cost-effective utilisation of starch requires consolidated bioprocessing (cbp) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol.201324286270
fungal diversity of rice straw for meju fermentation.rice straw is closely associated with meju fermentation and it is generally known that the rice straw provides meju with many kinds of microorganisms. in order to elucidate the origin of meju fungi, the fungal diversity of rice straw was examined. rice straw was collected from 12 jang factories where meju are produced, and were incubated under nine different conditions by altering the media (mea, drbc, and dg18), and temperature (15°c, 25°c, and 35°c). in total, 937 strains were isolated and ide ...201324043125
crystal structure of endo-xylogalacturonan hydrolase from aspergillus tubingensis.endo-xylogalacturonan hydrolase is a member of glycoside hydrolase family 28 (gh28) that hydrolyzes the glycosidic bond between two β-xylose-substituted galacturonic acid residues in pectin. presented here is the x-ray crystal structure of the endo-xylogalacturonan hydrolase from aspergillus tubingensis (xgha) at 1.75 å resolution. the high degree of structural conservation in the active site and catalytic apparatus compared with polygalacturonases indicates that cleavage of the substrate procee ...201324034788
biodegradation of polyester polyurethane by aspergillus tubingensis.the xenobiotic nature and lack of degradability of polymeric materials has resulted in vast levels of environmental pollution and numerous health hazards. different strategies have been developed and still more research is being in progress to reduce the impact of these polymeric materials. this work aimed to isolate and characterize polyester polyurethane (pu) degrading fungi from the soil of a general city waste disposal site in islamabad, pakistan. a novel pu degrading fungus was isolated fro ...201728318785
development of enzyme technology for aspergillus oryzae, a. sojae, and a. luchuensis, the national microorganisms of japan.this paper describes the modern enzymology in japanese bioindustries. the invention of takadiastase by jokiti takamine in 1894 has revolutionized the world of industrial enzyme production by fermentation. in 1949, a new γ-amylase (glucan 1,4-α-glucosidase, ec 3.2.1.3) from a. luchuensis (formerly designated as a. awamori), was found by kitahara. rnase t1 (guanyloribonuclease, ec 3.1.27.3) was discovered by sato and egami. ando discovered aspergillus nuclease s1 (single-stranded nucleate endonucl ...201627151561
discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen.sulfhydryl oxidases (sox) are fad-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. although the quantity of scientific papers and suggested applications for sox is constantly increasing, only a limited number of microbial sox have been reported and are commercially available. hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal sox. the screening was based on the ellman's reagent, i.e. 5,5 ...201323417429
optimization of inulinase production by a newly isolated aspergillus tubingensis cr16 using low cost substrates.production of an extracellular, thermostable inulinase was carried out by a newly isolated strain of aspergillus tubingensis cr16 using wheat bran and corn steep liquor (csl) under solid state fermentation (ssf). response surface methodology (rsm) involving box behnken design (bbd) was employed for the optimization of process parameters viz. time period of fermentation, % moisture content, inoculum size and ph of the medium. maximum yield of inulinase was 257±11.4 u/g, obtained by inoculating 5 ...201224751068
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