| production of amylase by a submerged culture of aspergillus wentii. | soluble starch was hydrolysed to maltose by aspergillus wentii wehmer (imi 17295). studies on nutritional requirements of aspergillus wentii for production of amylase revealed that the optimum conditions were achieved in fermentation culture medium containing 1% starch, and incubated at 20 degrees c for 3 days at ph 6.0. tryptophan was the best nitrogen source. the amylase activity was completely inhibited when 1 mm sodium iodoacetate was incorporated into the medium. with 10 mm sodium citrate t ... | 1978 | 23985 |
| ultraviolet induced mutations in aspergillus wentii. | aspergillus wentii (imi 17295) and its three nutritional mutant strains were irradiated with uv rays. new mutants obtained differed from the parent strains in colour of the conidia, growth factor requirements and amylase activity. arginine deficient strains showed greater amylase activity. | 1978 | 86282 |
| reaction of beta-d-glucosidase a3 from aspergillus wentii with d-glucal. | | 1979 | 389631 |
| relative effectiveness of mutagens on amylase production in aspergillus wentii. | | 1977 | 614190 |
| [effect of a stimulating factor formed by aspergillus wentii on exoprotease biosynthesis by a culture of aspergillus kanagawaensis]. | the object of this work was to study the composition of proteases synthesized by an active culture of aspergillus kanagawaensis (i) as well as that in the presence of a cultural broth of asp. wentii (ii) which did not produce exoproteases. enzyme complexes i and ii are similar in qualitative composition but complex ii has a higher proteolytic activity. the preparation containing the stimulating factor produced by asp. wentii has been separated by gel filtration and ultrafiltration. biosynthesis ... | 1978 | 745553 |
| emodin, a toxic metabolite of aspergillus wentii isolated from weevil-damaged chestnuts. | a diarrheagenic toxin from culture extracts of aspergillus wentii wehmer isolated from weevil-damaged chinese chestnuts was identified as emodin (2-methyl-4,5,7-trihydroxyanthraquinone). the orange-red, crystalline toxin (mp 255 to 257 c) showed ultraviolet absorption maxima in ethyl alcohol at 223, 250, 267, 290, and 442 nm, and infrared absorption maxima at 3,400 cm-1 (oh), 1,635, and 1,625 cm-1. chemical shifts and coupling constants of the proton magnetic resonance spectra of the a. wentii t ... | 1975 | 1147616 |
| cleavage of cardiac steroid glucosides by pure beta-glucosidase from aspergillus ventii. | electrophoretically pure beta-glucosidase (beta-glucoside glucohydrolase, ec 3.2.1.21) a3 from aspergillus wentii was shown to deglucosylate cardiac steroid biosides, triosides and tetraosides with a terminal beta-d-glucoside residue linked to the aglycon sugar in 1 leads to 6 or 1 leads to 4 position. in dependence on the structure and conformation of the aglycon, the deglucosylation rate may vary by six orders of magnitude. | 1975 | 1227250 |
| the interaction of 1-fluoro-d-glucopyranosyl fluoride with glucosidases. | 1. 1-fluoro-d-glucopyranosyl fluoride undergoes ph-independent loss of f- ion at a rate of 1 x 10(-8) s-1 at 50.0 degrees c, some 10(3)-fold slower than alpha-d-glucopyranosyl fluoride and 4 x 10(4)-fold slower than beta-d-glucopyranosyl fluoride. 2. the (inverting) amyloglucosidase ii of aspergillus niger hydrolyses the difluoride according to michaelis-menten kinetics (km 34 mm and kcat. 0.27 s-1), by apparently the same (simple) mechanism by which it hydrolyses alpha-d-glucopyranosyl fluoride ... | 1991 | 1953653 |
| localization of pyruvate carboxylase in organic acid-producing aspergillus strains. | the localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different aspergillus species (14 strains). in some species (a. aculeatus, a. flavus, a. foetidus, a. nidulans, a. ochraceus, and a. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of aspergillus wentii. in aspergillus oryzae and in five strains of aspergillus niger, ... | 1990 | 2383004 |
| nucleotide sequence of the clostridium thermocellum bgib gene encoding thermostable beta-glucosidase b: homology to fungal beta-glucosidases. | the nucleotide sequence of the bglb gene, coding for the thermostable beta-glucosidase b of clostridium thermocellum was determined. the coding region of 2265 bp was identified by comparison with the n-terminal amino acid sequence of beta-glucosidase b purified from escherichia coli. the derived amino acid sequence corresponding to a polypeptide of mr 84,100 was confirmed by sequencing of the c-terminal peptide generated by cleavage with cyanogen bromide. the protein bears no resemblance to othe ... | 1989 | 2505054 |
| sequence and transcription of the beta-glucosidase gene of kluyveromyces fragilis cloned in saccharomyces cerevisiae. | the complete nucleotide sequence of the beta-glucosidase gene of kluyveromyces fragilis has been determined. this sequence contains an open reading frame of 2535 base pairs encoding a protein of 845 amino acids. analysis of the transcription products revealed only one transcript of about 3 kb identical in both kluyveromyces fragilis and in the expression host saccharomyces cerevisiae. the protein molecular weight of 93,811 kd deduced from the sequence is consistent with the 90,000 kd determined ... | 1987 | 2835179 |
| synthesis of 5-amino-5-deoxy-d-galactopyranose and 1,5-dideoxy-1,5-imino-d-galactitol, and their inhibition of alpha- and beta-d-galactosidases. | a 12-step route is presented starting from 1,2:5,6-di-o-isopropylidene-alpha-d-glucofuranose for the preparation of the title compounds and their l-altro analogues. their synthesis is based on the reduction with raney nickel of a protected 5-hydroxyimino derivative of l-arabino-hexofuranos-5-ulose, with the following improvements for the preparation of a d-galactofuranose derivative: oxidation at c-3 with pyridinium dichromate-acetic anhydride, stereospecific reduction of a 3-o-acetyl-hex-3-enof ... | 1986 | 3024831 |
| isolation and amino acid sequence of a hexadecapeptide from the active site of beta-glucosidase a3 from aspergillus wentii. | | 1974 | 4611895 |
| toxic metabolite produced by aspergillus wentii. | mycelial extracts of an aspergillus wentii strain grown on yeast-extract sucrose medium and initially isolated from country-cured ham were highly toxic when inoculated into chicken embryos or fed to mice. moldy corn and rice were less toxic when fed to mice. water extracts of moldy corn or rice or culture filtrates from yeast-extract sucrose medium were not toxic. purification by thin-layer chromatography followed by crystallization yielded orange-red crystals that showed high toxicity and had a ... | 1974 | 4823420 |
| variability in amylase production among morphological mutants of aspergillus wentii. | | 1974 | 4855489 |
| composition, n-terminal amino acids, and chain length of a -glucosidase from aspergillus wentii. | | 1972 | 5009834 |
| production of aflatoxin by aspergillus wentii wehmer. | | 1969 | 5344742 |
| [on the mechanism of action of glycoside-splitting enzymes. vi. catalytically active groups of a beta-glucosidase from aspergillus wentii]. | | 1970 | 5425946 |
| [studies on the mechanism of action of glycoside-splitting enzymes, ii. isolation and enzymatic properties of 2 beta-glucosidases from aspergillus wentii]. | | 1967 | 5586469 |
| [investigations on the mechanism of action of glycoside-splitting enzymes. 3. labelling of the active center of a beta-glucosidase from aspergillus wentii with (14c) conduritol b epoxide]. | | 1968 | 5722821 |
| synthesis of 5-amino-5-deoxy-d-mannopyranose and 1,5-dideoxy-1,5-imino-d-mannitol, and inhibition of alpha- and beta-d-mannosidases. | the title compounds and the corresponding l-gulo derivatives were synthesised in 6 steps from benzyl 2,3:5,6-di-o-isopropylidene-alpha-d-mannofuranoside. the ki values, determined from inhibition studies with alpha-d-mannosidases from jack beans, almonds, and calf liver, and beta-d-mannosidase from aspergillus wentii, ranged from 70 to 400 microm for the mannitol derivative and from 1.2 to 20 microm for 5-amino-5-deoxy-d-mannopyranose, i.e., inhibition is 10(2)-10(4)-fold stronger than with d-ma ... | 1984 | 6234061 |
| [isolation and study of the biological properties of a protease biosynthesis stimulating factor in an associative fungal culture]. | a factor stimulating the production of exoproteases by aspergillus kanagawaensis both in a single culture and during combined cultivation with aspergillus wentii was isolated from the cultural broth of a component in the microbial association according to our scheme. the factor is a pigment of the hydroxyanthraquinone nature. it made the enzyme biosynthesis increase 3.7 times. its effect on the biosynthesis was compared to that produced by other anthraquinone pigments, viz. alizarin, rubomycin a ... | 1983 | 6363889 |
| [occurrence and analysis of the mycotoxin emodin]. | besides other mould species, aspergillus wentii wehmer is assumed to form the hydroxyanthraquinone derivative emodin which has toxic and gene mutagenic properties. as a pectinase producer this fungus is used for the industrial isolation of pectinolytic enzymes for the food industry so that a contamination of such preparations by emodin cannot be excluded. however, preliminary thin-layer chromatographic studies of extracts of the corresponding pectinolytic enzyme preparations and aspergillus went ... | 1984 | 6717559 |
| isolation and structure of a tryptic glycopeptide from the active site of beta-glucosidase a3 from aspergillus wentii. | a radioactive glycopeptide with a molecular weight of 13 200 was isolated from beta-glucosidase a3 after labeling the active site with [3h]conduritol b epoxide and cleavage with trypsin. the glycopeptide consists of 63 amino acids and 29 +/- 1 sugar residues. its amino acid sequence was derived from the results of sequence analysis of peptic and cyanogen bromide peptides. the radioactive inhibitor is bound to aspartic acid 12 of the sequence, the sugar residues are probably bound as n-glycosides ... | 1980 | 6783081 |
| role of sugar hydroxyl groups in glycoside hydrolysis. cleavage mechanism of deoxyglucosides and related substrates by beta-glucosidase a3 from aspergillus wentii. | the contribution of the hydroxyl groups at c-2 and c-4 and of the hydroxy-methyl group at c-5 of beta-glucopyranosides to their hydrolysis by beta-glucosidase a3 (beta-d-glucoside glucohydrolase, ec 3.2.1.21) from aspergillus wentii was investigated with 4-methylumbelliferyl-beta-glucosides with appropriate structural modifications. relative hydrolysis rates expressed by kcat/kcat (glucoside) are: 2-deoxy, 4. 10(-6); 2-deoxy-2-amino, 2.4 . 10(-4); 2-deoxy-2-ammonio, less than 1 . 10(-6); 4-deoxy ... | 1981 | 6783098 |
| [role of an exoprotease biosynthesis-stimulating microbial factor in aspergillus kanagawaensis in the process of its growth and development]. | morphofunctional changes were studied in the culture of aspergillus kanagawaensis producing exoproteases; the changes accompanied an increase in the rate of biosynthesis of these enzymes in the presence of a stimulating factor produced by aspergillus wentii. the germination of conidia and the formation of the secondary mycelium in a. kankagawaensis were delayed by 6--12 hours under the action of the stimulating factor. the monoculture of the fungus producing exoproteases is characterized by the ... | 1980 | 7010090 |
| new secondary metabolites of aspergillus wentii wehmer. the positive and negative ion mass spectra produced by electron impact. | the positive and negative ion mass spectra of three secondary metabolites of aspergillus wentii wehmer, namely 15-acetoxyhexadecyl-, 15-oxohexadecyl- and 15-hydroxyhexadecylcitraconic anhydrides are reported and discussed. the positive ion mass spectra, which have been studied in detail by exact mass measurements and metastable ion detection using a defocusing method, show very weak molecular ions and complex fragmentation pathways, strongly influenced by the group on c-15. however, the negative ... | 1980 | 7448329 |
| interactions among xerophilic fungi associated with dried salted fish. | interactions were investigated among five xerophilic fungi, polypaecilum pisce, basipetospora halophila, eurotium rubrum, aspergillus wentii and a. penicillioides, isolated from indonesian dried salted fish. a range of water activities (aw) (0.98, 0.95, 0.90 and 0.84) and temperatures (15 degrees, 25 degrees and 30 degrees c) were studied on agar media in petri dishes, and with dried fish as a substrate at 0.90 and 0.84 aw at 30 degrees c. generally, the fungi exhibited one of two interaction ty ... | 1993 | 8444646 |
| microbial degradation of aquatic biomass by trichoderma viride 992 and aspergillus wentii 669 with reference to the physical structure. | there was a considerable change in the structural parameters of both the lignocellulosic substrates. this change was confirmed by infrared (ir) spectroscopy and x-ray diffraction data, which revealed the presence of phenolic groups in the peaks range 1310-1410 and 1200 cm-1 with large crystalline substrates. these were found to be reduced after treatment with alkali. enzyme/s activity were found to be increased after pretreatment of hydrolysed substrates. the structural modifications in both the ... | 1993 | 8478790 |
| detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes. | aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ... | 1997 | 9458383 |
| studies on toxigenic fungi in roasted foodstuff (salted seed) and halotolerant activity of emodin-producing aspergillus wentii. | commercial roasted salted peanuts (3% nacl), popcorn (1% nacl), summer-squash (9% nacl), sunflower (3% nacl) and wild-melon (3% nacl) seeds are polluted with fungi, mostly aspergillus flavus, a. niger, penicillium chrysogenum, p. corylophilum and rhizopus stolonifer. contamination of popcorn with the fungi is about 10 times higher than in the other foods. these fungi, common also on unsalted seeds, are significantly inhibited in seeds (30% moisture content) treated with 9-21% nacl. the halotoler ... | 1998 | 9821293 |
| [effect of trace metals on cell morphology, enzyme activation, and production of citric acid in a strain of aspergillus wentii]. | data concerning the effect of very low concentrations of metals on citric acid production by microorganisms, as well as on the activity of enzymes presumptively involved in the process, are confuse. the bulk of information was obtained mainly studying selected strains of aspergillus niger. information concerning other citric acid producer filamentous fungi, such as a. wentii, is scanty. in the present article we report the effect of different cations on the growth pattern of a. wentii p1 as well ... | 1999 | 10425661 |
| cloning, expression, characterization, and nucleophile identification of family 3, aspergillus niger beta-glucosidase. | the beta-glucosidase from aspergillus niger (cmi cc 324262) was purified, and an n-terminal sequence and two internal sequences were determined. bgli genomic gene and the cdna were cloned from a genomic library and by reverse transcriptase-polymerase chain reaction, respectively. the cdna was successfully expressed in saccharomyces cerevisiae and pichia pastoris. sequence analysis revealed that the gene encodes a 92-kda enzyme that is a member of glycosidase family 3. (1)h-nmr analysis of the re ... | 2000 | 10671536 |
| ammonium oxidation by cell-free extracts of aspergillus wentii. | | 1964 | 14209400 |
| differences among the cell wall galactomannans from aspergillus wentii and chaetosartorya chrysella and that of aspergillus fumigatus. | the alkali extractable and water-soluble cell wall polysaccharides f1ss from aspergillus wentii and chaetosartorya chrysella have been studied by methylation analysis, 1d- and 2d-nmr, and maldi-tof analysis. their structures are almost identical, corresponding to the following repeating unit: [--> 3)-beta-d-gal f -(1 --> 5)-beta-d-gal f-(1 -->]n --> mannan core. the structure of this galactofuranose side chain differs from that found in the pathogenic fungus aspergillus fumigatus, in other asper ... | 2004 | 15115908 |
| method to estimate growth of trichoderma reesei and aspergillus wentii in mixed culture on cellulosic substrates. | a simple differential method based on measurement of an intracellular pigment of aspergillus wentii was developed for estimation of the individual growths of two fungi, trichoderma reesei and a. wentii, in mixed-culture fermentation of an insoluble cellulosic substrate. | 1989 | 16347888 |
| starch hydrolysis by conidia of aspergillus wentii. | soluble starch was hydrolyzed to glucose by conidia of aspergillus wentii nrrl 2001. peak yields of glucose were achieved in 3 days. a glucoamylase-like enzyme was assumed to be responsible since maltose was not detected during the conversion. spore age, storage conditions, and temperature affected the level of glucose accumulated. iodoacetate inhibited catabolism of the glucose formed and this inhibition increased product yield. spores of other fungi also hydrolyzed starch but none accumulated ... | 1968 | 16349820 |
| effect of culture phasing and mannanase on production of cellulase and hemicellulase by mixed culture of trichoderma reesei d 1-6 and aspergillus wentii pt 2804. | significant increase in extracellular cellulase and hemicellulase activities was observed in the biosynthesis of cellulase enzyme in mixed culture fermentation of trichoderma reesei d 1-6 and aspergillus wentii pt 2804 when the a. wentii inoculation was phased by 15 h. the optimal conditions of fermentation by the mixed culture have been established. presence of mannanase has been found to affect the release as well as activity of cellulase enzyme produced in mixed culture. | 1985 | 18553825 |
| effect of culture phasing and a polysaccharide on production of xylanase by mixed culture of trichoderma reesei d1-6 and aspergillus wentii pt 2804. | a significant increase in extracellular xylanase activity was observed in the mixed culture fermentation of trichoderma reesei d1-6 and aspergillus wentii pt 2804 when a. wentii inoculation was phased by 15 h. a. wentii produced a polysaccharide, chiefly consisting of glucose monomeric units, which was required for expression of maximum xylanase activity. expression of high activity of xylanase in the a. wentii phased mixed culture compared to that in either t. reesei or a. wentii single culture ... | 1987 | 18581522 |
| necrotising external otitis caused by aspergillus wentii: a case report. | summary necrotising external otitis (neo) is a destructive, potentially fatal, infection usually seen in elderly diabetics or the immunocompromised. the commonest causative organism is pseudomonas but immunocompromised patients are additionally susceptible to opportunistic infections. here we describe the first reported case of neo caused by a previously unknown human pathogen -aspergillus wentii. a review of the literature reveals that fungal neo is associated with a high rate of cranial nerve ... | 2010 | 20059696 |
| biotransformation of some steroids by aspergillus wentii. | first steroid biotransformations performed by aspergillus wentii mrc 200316 are reported. testosterone (1) yields 6beta-hydroxytestosterone (3) and 14alpha-hydroxytestosterone (4) while progesterone (2) yields 11alpha-hydroxyprogesterone (5). | 2010 | 21319711 |
| in vitro antioxidant potential of some soil fungi: screening of functional compounds and their purification from penicillium citrinum. | fungal isolates (aspergillus wentii 1, a. wentii 2, penicillium citrinum, penicillium granulatum) were selected to study their in vitro antioxidant potential by various assay procedures. czapek-dox's medium was selected for the growth of fungi as it supported the best antioxidant activity based on their ec(50) values, p. citrinum was the best followed by p. granulatum, a. wentii 1, and a. wentii 2. the chromatographic analyses showed several compounds possessing antioxidant activity in the funga ... | 2011 | 21603952 |
| does fungicide application in vineyards induce resistance to medical azoles in aspergillus species? | this study assessed if the use of sterol demethylase inhibitor fungicides in vineyard production can induce resistance to azoles in aspergillus strains and if it can induce selection of resistant species. we also tried to identify the aspergillus species most prevalent in the vineyards. two vineyards from northern portugal were selected from "vinhos verdes" and "douro" regions. the vineyards were divided into plots that were treated or not with penconazole (pen). in each vineyard, air, soil, and ... | 2014 | 24833021 |
| population structure and aflatoxin production by aspergillus sect. flavi from maize in nigeria and ghana. | aflatoxins are highly toxic carcinogens that contaminate crops worldwide. previous studies conducted in nigeria and ghana found high concentrations of aflatoxins in pre- and post-harvest maize. however, little information is available on the population structure of aspergillus sect. flavi in west africa. we determined the incidence of aspergillus sect. flavi and the level of aflatoxin contamination in 91 maize samples from farms and markets in nigeria and ghana. aspergillus spp. were recovered f ... | 2014 | 24750813 |
| exposure assessment of lovastatin in pu-erh tea. | this paper reports the results of an extensive survey on the levels of lovastatin in pu-erh tea samples. the microbial source of lovastatin was assessed by testing the ability of fungi with higher isolation frequency in the pu-erh tea samples to produce lovastatin on czapek yeast extract agar (cya). lovastatin was not detected in any of the raw pu-erh tea samples without storage but was found in almost all the ripe pu-erh tea samples, with lovastatin contents ranging from 20.61 ng/gdw to 226.38 ... | 2013 | 23587710 |
| tetranorlabdane diterpenoids from the deep sea sediment-derived fungus aspergillus wentii sd-310. | two new tetranorlabdane diterpenoids, asperolides d (1) and e (2), along with six related known congeners (3-8), were isolated and identified from the culture extract of the deep sea sediment-derived fungus aspergillus wentii sd-310. the structures of these compounds were established on the basis of spectroscopic interpretation, and the skeleton and absolute configurations of asperolides d (1) and e (2) were determined by x-ray crystallographic analysis. compounds 1 and 2 were evaluated for thei ... | 2016 | 27257768 |
| aspewentins d-h, 20-nor-isopimarane derivatives from the deep sea sediment-derived fungus aspergillus wentii sd-310. | five new 20-nor-isopimarane diterpenoids, aspewentins d-h (1-5), along with a related known congener, aspewentin a (6), were isolated from the culture extract of aspergillus wentii sd-310, a fungal strain obtained from a deep-sea sediment sample. the structures of these compounds were established on the basis of spectroscopic interpretation, and the absolute configurations of compounds 1-5 were determined by x-ray crystallographic analysis and tddft-ecd calculations. the isolated compounds were ... | 2016 | 27148955 |
| extraordinary solute-stress tolerance contributes to the environmental tenacity of mycobacteria. | mycobacteria are associated with a number of well-characterized diseases, yet we know little about their stress biology in natural ecosystems. this study focuses on the isolation and characterization of strains from yellowstone national park (ynp) and glacier national park (gnp; usa), the majority of those identified were mycobacterium parascrofulaceum, mycobacterium avium (ynp) or mycobacterium gordonae (gnp). generally, their windows for growth spanned a temperature range of > 60 °c; selected ... | 2015 | 26059202 |
| concomitant osmotic and chaotropicity-induced stresses in aspergillus wentii: compatible solutes determine the biotic window. | whereas osmotic stress response induced by solutes has been well-characterized in fungi, less is known about the other activities of environmentally ubiquitous substances. the latest methodologies to define, identify and quantify chaotropicity, i.e. substance-induced destabilization of macromolecular systems, now enable new insights into microbial stress biology (cray et al. in curr opin biotechnol 33:228-259, 2015a, doi: 10.1016/j.copbio.2015.02.010 ; ball and hallsworth in phys chem chem phys ... | 2015 | 26055444 |
| isolation and evaluation of native cellulose degrading microorganisms for efficient bioconversion of weed biomass and rice straw. | cellulose decomposing microorganisms (cdms) are important for efficient bioconversion of plant biomasses. to this end, we isolated seven fungal isolates (aspergillus wentii, fusarium solani, mucor sp., penicillum sp., trichoderma harzaianum, trichoderma sp.1 and trichoderma sp.2) and three bacterial isolates (bacterial isolate i, ii and iii) from partially decomposed farm yard manure, rice straw and vermicompost, and evaluated them for decomposition of rice straw (oryza sativa), ipomoea camea an ... | 2014 | 25004759 |
| aspewentins a-c, norditerpenes from a cryptic pathway in an algicolous strain of aspergillus wentii. | through addition of suberoylanilide hydroxamic acid, two new aromatic norditerpenes, aspewentins a (1) and b (2), along with an oxygenated derivative, aspewentin c (3), were obtained from the culture of an aspergillus wentii strain (na-3) isolated from the tissue of the brown alga sargassum fusiforme. the structures and absolute configurations were unambiguously elucidated by spectroscopic analyses and quantum chemical calculations. aspewentins a-c were produced before sporulation and exhibited ... | 2014 | 24499164 |
| wentilactone b induces g2/m phase arrest and apoptosis via the ras/raf/mapk signaling pathway in human hepatoma smmc-7721 cells. | hepatocellular carcinoma (hcc) is generally acknowledged as the most common primary malignant tumor, and it is known to be resistant to conventional chemotherapy. wentilactone b (wb), a tetranorditerpenoid derivative extracted from the marine algae-derived endophytic fungus aspergillus wentii en-48, has been shown to trigger apoptosis and inhibit metastasis in hcc cell lines. however, the mechanisms of its antitumor activity remain to be elucidated. we report here that wb could significantly ind ... | 2013 | 23744357 |
| three new xanthone derivatives from an algicolous isolate of aspergillus wentii. | three new xanthone derivatives, yicathin a (1), yicathin b (2), and yicathin c (3), and three known anthraquinone derivatives, alatinone (4), 1,5-dihydroxy-3-methoxy-7-methylanthraquinone (5), and 5-hydroxy-1,3-dimethoxy-7-methylanthraquinone (6), were isolated from the cultures of aspergillus wentii pt-1, an endophytic fungus isolated from the marine red alga gymnogongrus flabelliformis. their structures were unambiguously elucidated by nmr and mass spectroscopic methods as well as quantum chem ... | 2013 | 23172582 |
| chemical characterization of a water insoluble (1→3)-α-d-glucan from an alkaline extract of aspergillus wentii. | the chemical structure of a water insoluble α-glucan isolated from the cell wall of aspergillus wentii was described on the basis of total acid hydrolysis, methylation analysis, and 1d and 2d nmr studies (tocsy, dqf-cosy, noesy and hsqc) as well as other instrumental techniques. it was established that the analyzed preparation contained a linear polymer composed almost exclusively of (1→3)-linked α-d-glucose, with a molecular mass of about 850kda. the polymer was divided into subunits separated ... | 2013 | 23121952 |
| wentilactone b from aspergillus wentii induces apoptosis and inhibits proliferation and migration of human hepatoma smmc-7721 cells. | wentilactone b (en-48-57) is one of the six derivatives separated from aspergillus wentii (en-48). of these derivatives, wentilactone b exerted a more significant antibacterial and cytotoxic activity in several tumor cell lines. the present study demonstrates that wentilactone b could efficiently induce smmc-7721 cells apoptosis, but not normal hepatic cells, as measured by an inverted microscope, 4',6-diamidino-2-phenylindole staining and flow cytometry. in addition, wentilactone b could inhibi ... | 2012 | 22972522 |
| asperolides a-c, tetranorlabdane diterpenoids from the marine alga-derived endophytic fungus aspergillus wentii en-48. | bioassay-guided fractionation of the culture extract of aspergillus wentii en-48, an endophytic fungus isolated from an unidentified marine brown algal species of the genus sargassum, led to the isolation of three new tetranorlabdane diterpenoids, asperolides a-c (1-3), and five related derivatives (4-8). the structures of these compounds were established on the basis of spectroscopic interpretation, and compound 1 was confirmed by x-ray crystallographic analysis. the absolute configuration of 1 ... | 2012 | 22283451 |