the structures of five new didehydropeptides related to neoechinulin, isolated from aspergillus amstelodami. 1977558214
[synthetic studies on echinulin and related natural products. iv. isolation, structure and synthesis of flavoglaucin-auroglaucin type natural products isolated from aspergillus amstelodami (author's transl)]. 1977560464
[synthetic studies on echinulin and related natural products. v. isolation, structure and synthesis of echinulin-neoechinulin type alkaloids isolated from aspergillus amstelodami (author's transl)]. 1977560465
the electron impact induced fragmentation of aspergillus amstelodami alkaloids and derivatives.the mass spectral data of four isoprenylated indole alkaloids containing a piperazinedione group, neoechinuline, cryptoechinuline c, and a and echinuline, isolated from aspergillus amstelodami cultures, and of two derivatives obtained by catalytic hydrogenation of neoechinuline and echinuline, are reported and discussed. their fragmentation patterns are strongly dependent upon the presence of a single or a double bond betueen c-8 and c-9.1977890072
how relevant are house dust mite-fungal interactions in laboratory culture to the natural dust system?both house dust and house dust mites dermatophagoides pteronyssinus contained a wider range of fungi than laboratory mite cultures. in total, nine species of fungi were isolated from d. pteronyssinus in house dust, and these included three xerophilic species (eurotium amstelodami, aspergillus penicillioides and wallemia sebi) commonly found in laboratory cultures of d. pteronyssinus. it is concluded that mites do interact with a similar range of fungi in natural dust and in laboratory culture, b ...19921493747
cloning and sequencing of the melb gene encoding the melibiose permease of salmonella typhimurium lt2.the nucleotide sequence of the melb gene coding for the na+ (li+)/melibiose symporter of salmonella typhimurium lt2 was determined, and its amino acid sequence was deduced. it consists of 1428 bp, corresponding to a protein of 476 amino acid residues (calculated molecular weight 52,800). the amino acid sequence is homologous to that of the melibiose permease of escherichia coli k12, with 85% identical residues. all, except one, of the amino acid residues that have been reported to be important f ...19921495487
keratinophilic fungi and other moulds associated with air-dust particles from and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees c. using the hair-baiting technique with horse hair, 10 species of chrysosporium were isolated: c. asperatum, c. state of arthroderma tuberculatum, c. indicum, c. inops, c. keratinophilum, c. merdarium, c. pannorum, c. queenslandicum, c. tropicum and c. xerophilum. true dermatophytes were isolated: trichophyton verrucosum and trichophyton sp. ...19901702081
potential for the development of tolerance by aspergillus amstelodami, a. repens and a. ruber after repeated exposure to potassium sorbate.three strains of a. amstelodami, a. repens and a. ruber were exposed to various levels of potassium sorbate, and the mics were determined. selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compound to determine whether increased tolerance might develop. the mic of sorbate (ph 5.5 or 6.5) for 3 species of aspergillus was 0.07%. increasing levels of sorbate resulted in increasing growth suppression of the molds. the 3 aspergillus species were tested for incr ...19902371795
mitochondrial dna of chlamydomonas reinhardtii: the nd4 gene encoding a subunit of nadh dehydrogenase.the nd4 gene encoding a subunit of respiratory nadh dehydrogenase has been identified on the linear 15.8 kb mitochondrial dna of chlamydomonas reinhardtii. the gene maps downstream of nd5. the 1,332 bp nucleotide sequence presented is the first complete reported nd4 sequence from a photoautotrophic organism. the deduced protein of 443 amino acid residues shows 34%, 29% and 27% homology to the protein sequences of aspergillus amstelodami, drosophila yakuba and mouse, respectively. nd4 is the fift ...19892791036
behaviour of a replicating mitochondrial dna sequence from aspergillus amstelodami in saccharomyces cerevisiae and aspergillus amplified sequence of mitochondrial dna from a ragged (rgd) mutant of aspergillus amstelodami has been shown to exist in multimeric circular form, suggesting that it is excised from the genome and can exist independently of it. this sequence has replicative (ars) activity in saccharomyces cerevisiae, and a subfragment responsible for this activity has been identified and sequenced. a homologous sequence from aspergillus nidulans mtdna also has ars activity in s. cerevisiae. both a. amstelodam ...19883042169
prevalence of airborne aspergillus flavus in khartoum (sudan) airspora with reference to dusty weather and inoculum survival in simulated summer conditions.khartoum air was scanned for airborne aspergillus flavus for 12 months using the horizontal gravitational settling method. frequency of occurrence was related to total fungal catch and dusty weather. the aspergilli were prevalent (68% of total isolated/plate/month) and a. flavus constituted 31% of the total aspergilli. in june (hot, dry & dusty) aspergilli constituted 79% of the total isolates, whilst a. flavus represented 30% from amongst the other aspergilli. a. flavus, a. niger, a. nidulans ( ...19883148861
water relations of paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, xerophilic fungi isolated from indonesian dried fish.the water relations of four xerotolerant fungi, paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, isolated from dried salt fish, were examined at 25 degrees c, on media in which water activity (aw) was controlled by nacl or a glucose/fructose mixture. all fungi were less tolerant of nacl than glucose/fructose at low aw. p. variotii grew 2 to 3 times faster on glucose/fructose media than on nacl. the minimum aw permitting germination varied from 0.753 for ...19883275312
eurotium spp. and echinulin in feed refused by swine.feed refused by swine contained a high-propagule density of eurotium chevalieri mangin (anamorph, aspergillus chevalieri (mangin) thom and church), eurotium amstelodami mangin (anamorph, aspergillus amstelodami (mangin thom and church), and aspergillus candidus link. echinulin (8 micrograms/g of feed) was detected in the feed. isolates of e. chevalieri and e. amstelodami but not a. candidus produced echinulin on rice or cracked corn. mice refused to drink water containing 90 micrograms of echinu ...19883377495
isoprene-containing metabolites of aspergillus amstelodami. 19724340780
correlated meiotic and mitotic maps in aspergillus amstelodami. 19695367372
biosynthesis of echinulin by aspergillus amstelodami from cyclo-l-alanyl-l-tryptophyl-14c. 19705460486
amplification of a mitochondrial dna sequence in the cytoplasmically inherited 'ragged' mutant of aspergillus amstelodami.a comparison has been made between mtdna of the cytoplasmically inherited 'ragged' mutant of aspergillus amstelodami and that of the wild-type strain. ragged mitochondria contain both the wild-type mitochondrial genome and several large dna molecules which are not cleaved by the restriction endonucleases bamhi, haeiii, hhai, hindii, hindiii, psti and mboi, but are converted by either ecori or hpaii into a single 820-840 base-pair fragment. restriction analysis and molecular hybridization data in ...19806249580
detection of fungi in cerebrospinal fluid.with the exception of cryptococcus neoformans, fungi are rarely detected in cerebrospinal fluid obtained from patients having or suspected of having fungal meningitis. a review of the literature reveals that several fungi have been either isolated, observed, or both in cerebrospinal fluid specimens. these fungi include acremonium species, aspergillus amstelodami, a. flavus, a. fumigatus, a. oryzae, a. terreus, blastomyces dermatitidis, candida albicans, c. tropicalis, c. viswanathii, coccidioide ...19836349340
lack of mutagenic effects on conidia of aspergillus amstelodami irradiated by 8.7175-ghz cw microwaves.conidial suspensions of aspergillus amstelodami were exposed to 8.7175-ghz cw microwaves in the near field of a horn antenna. conidia were irradiated for 3- and 6-hour time intervals and at a temperature ranging between 28 and 30 degrees c. the density of microwave power incident on the suspension was 2.09 mw/cm2. two types of mutant, conidial colour variants and 8-azaguanine resistant mutants, were looked for. the treatment produced no significant change in the frequencies at which the two clas ...19826926529
biocontrol of mold growth in high-moisture wheat stored under airtight conditions by pichia anomala, pichia guilliermondii, and saccharomyces cerevisiae.pichia anomala inhibits the growth of penicillium roqueforti and aspergillus candidus on agar. in this investigation, antagonistic activity on agar against 17 mold species was determined. the abilities of pichia anomala, pichia guilliermondii, and saccharomyces cerevisiae to inhibit the growth of the mold penicillium roqueforti in nonsterile high-moisture wheat were compared by adding 10(3) penicillium roqueforti spores and different amounts of yeast cells per gram of wheat. inoculated grain was ...19957793907
lipolytic activity and degradation of rapeseed oil and rapeseed by spoilage fungi.aspergillus, eurotium and penicillium spp. from rapeseed were able to grow and produce lipases over a range of water activities (aw) at both 15 and 25 degrees c on tributyrin agar. the ability to produce lipases was not directly related to growth rate. the clearing zone: growth rate ratios gave lipase indices which varied markedly between test fungi and with aw. the fungi with the highest indices were aspergillus candidus and aspergillus versicolor and penicillium expansum and penicillium hordei ...19938217518
survey of mycoflora and mycotoxins of some dried fruits in egypt.fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-czapek's agar medium at 28 +/- 2 degrees c. penicillium, aspergillus and cladosporium were the common genera on the two types of media used. a ...19938229671
evaluation of modified dichloran 18% glycerol (dg18) agar for enumerating fungi in wheat flour: a collaborative study.dichloran 18% glycerol agar base supplemented with 100 micrograms of chloramphenicol ml-1 (dg18 agar) was compared to dg18 agar supplemented with 100 micrograms of triton x-301 ml-1 (dg18t) and dg18 agar supplemented with 1 microgram of iprodione [3-(3,5-dichlorophenyl)-n-(1-methyl-ethyl)-2,4-dioxo-1-imidazolidine- carboxamide] ml-1 (dg18i agar) for enumeration of fungi in ten brands of wheat flour. as the flours contained low fungal populations, all were inoculated with two to four strains of x ...19968796418
effect of water activity, modified atmosphere packaging and storage temperature on spore germination of moulds isolated from prunes.the combined effect of medium water activity (aw) modified atmosphere packaging (map) on the conidial germination of aspergillus niger, eurotium amstelodami, penicillium chrysogenum and fusarium oxysporum was studied by applying response surface methodology (rsm) for 45 days incubation at 20, 30 and 40 degrees c. oxytetracycline glucose yeast extract (ogye) agar media were prepared over a wide range of aw (0.80-0.95), controlled by glucose. the headspace atmosphere composition varied from 0-20% ...19979081224
fungal microflora biodiversity as a function of pollution in oued sebou (morocco).an inventory of the fungal microflora present in sediments collected at 11 sites along oued sebou has been established. the influence of some climatic parameters (temperature, rainfall) and of pollution on the composition of the fungal communities has been questioned. emericella nidulans, eurotium amstelodami, neosartotya fisheri var. glabrum (ascomycetes), dreschslera biseptata (dematiaceae), penicillium citrinum (mucedinaceae) and phoma sp. (sphaeropsidale) can be considered as strains sensiti ...19979253167
concentration- and time-dependent upregulation and release of the cytokines mip-2, kc, tnf, and mip-1alpha in rat alveolar macrophages by fungal spores implicated in airway inflammation.inhalation of fungal spores has been shown to cause primary or secondary infection and respiratory inflammation and diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome, which are rarely reported in the absence of predisposing factors. biochemical and molecular markers of inflammation were measured in rat bronchial alveolar lavage cells (> 95% macrophages) following stimulation with fungal spores isolated from pathogenic and nonpathogenic fungi that have been impl ...19989490662
water activity and temperature effects on germination and growth of eurotium amstelodami, e. chevalieri and e. herbariorum isolates from bakery products.this study investigated the effect of temperature (5-30 degrees c), water activity (0.775-0.90 aw) and their interactions on the temporal rates of germination and mycelial growth of three species of eurotium on flour wheat sucrose medium. germination was quite rapid at aw >0.85, with an almost linear increase with time for all isolates. however, under more extreme water stress, germination was slower. the aw minima for germination were usually lower than those for growth and varied with temperat ...199910540239
water activity and temperature effects on growth of eurotium amstelodami, e. chevalieri and e. herbariorum on a sponge cake analogue.eurotium is a widespread storage fungal genus that has been frequently isolated from bakery products. the objective of this study was (i) to obtain a method for studying the growth of xerophilic fungi on bakery products, and (ii) to determine the effects of water activity (a(w)), temperature, isolate and their interactions on mycelial growth of eurotium spp. on an analogue medium of sponge cake. statistical analysis showed that there were intra-isolate differences (p<0.001) due to a(w), temperat ...199910573396
microbial populations, ammonification and nitrification in soil treated with urea and inorganic salts.soil fertilization with urea at rates of 0.2 and 0.5 mg n/g soil was toxic for total counts of bacteria and fungi except with cellulolytic fungi where growth was promoted by addition of urea after 90-d incubation. also, the population numbers of both bacteria and fungi were significantly decreased when soil was amended with cacl2 and k2so4 applied at two levels (50 and 100 mumol/g soil). some alleviation of the toxic effect of either urea or the inorganic salts was observed when they were applie ...199910588055
tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages.studies from our laboratory (shahan, t. a., sorenson, w. g., and lewis, d. m. (1994) environ. res. 67, 98-104) demonstrated that spores from different fungal species differentially activate rat alveolar macrophages as detected by the measurement of superoxide anion and cytokine production (shahan, t. a., siegel, p. d., sorenson, w. g., kuschner, w. g., and lewis, d. m. (1998) am. j. respir. cell mol. biol. 18, 435-441). spores from aspergillus candidus stimulated production of the highest levels ...200010744701
effect of modified atmosphere packaging and water activity on growth of eurotium amstelodami, e. chevalieri and e. herbariorum on a sponge cake analogue.the combined effects of water activity (aw), oxygen (o2) and carbon dioxide (co2) levels on growth variables of three species of eurotium (two isolates of each) on a sponge cake analogue were studied. the use of a multivariate statistical method (pls) for the analysis of data showed that in general, the fungi had the same behaviour, according to their growth responses, to changes in the three tested factors. however, the maximal growth of e. herbariorum was somewhat more affected by the o2 level ...200010792518
impact of environment and interspecific interactions between spoilage fungi and aspergillus ochraceus on growth and ochratoxin production in maize grain.using layers of irradiated but still fertile maize grain, the effects of water activity (0.995, 0.95 a(w)) and temperature (18, 30 degrees c) on interspecific interactions between aspergillus ochraceus and five other spoilage fungi were examined. asp. ochraceus was not competitive against asp. flavus, asp. niger, or alternaria alternata at 18 degrees c when water was freely available (0.995 a(w)), while at 0.95 a(w) it was dominant against asp. candidus, asp. flavus and alt. alternata. at 30 deg ...200011028955
impact of environmental factors on fungal respiration and dry matter losses in wheat automatic electrolytic respirometer enabled replicated determinations of the respiration rates of individual fungi on sterile straw, and the mixed mycoflora of naturally contaminated wheat straw at different steady-state temperatures (10-30 degrees c) and water activities (a(w), 0.75-0.98) over periods of 8-14 days. generally, the respiratory activity of individual spoilage fungi (alternaria alternata, cladosporium cladosporioides, eurotium amstelodami, fusarium culmorum and penicillium auran ...200011124368
a cardinal model to describe the effect of water activity on the growth of moulds.a simple model was proposed to describe the effect of water activity (aw) on the radial growth rate of moulds. this model is deduced from the cardinal model family proposed by rosso in 1995, which is only defined from cardinal values of environmental factors (minimum, optimum and maximum values), the growth rate observed at the optimal value of the environmental factors, and n, a shape parameter. for aw, a simple form of cardinal model is proposed. this form is obtained for n = 2 and aw(max) = 1 ...200111246910
role of molds in farmer's lung disease in eastern france.farmer's lung disease (fld) is common in the east of france. in the absence of the primary recognized fld agent, saccharopolyspora rectivirgula, its etiology remains unknown. a prospective case-control study was performed to find the etiology of fld in this area. eleven patients were matched with 11 healthy control farmers. twenty-two urban subjects constituted the nonexposed control group. microorganisms from cowshed air and fodder were identified and counted. the antigens of the microorganisms ...200111401869
occurrence of xerophilic fungi in bakery gingerbread production.the production process of a coconut type of gingerbread was examined for possible sources of contamination with xerophilic fungi. in the production flow chart, critical control points were found at which some effective steps could be made to eliminate undesirable fungal contamination, affected by water activity and temperature, and to prevent mould spoilage of the products. the following xerophilic fungi were detected, isolated and identified: eurotium amstelodami, e. chevalieri, e. herbariorum, ...200211843418
influence of buffered propionic acid on the development of micro-organisms in hay.we tested the benefit of using buffered propionic acid (bpa) as a means of preventing farmer's lung disease (fld). bpa, a new formulation of propionic acid, a hay preservative with no deleterious effect on farm machinery or cattle, reduces the development of micro-organisms in hay. twenty pairs of round bales were analysed for concentration of micro-organisms measured in the winter following hay treatment. each pair included one untreated bale and one bale treated with bpa during haymaking. our ...200212100537
identity of the xerophilic species aspergillus penicillioides: integrated analysis of the genotypic and phenotypic characters.we examined the identity of aspergillus penicillioides, the typical xerophilic and strictly anamorphic species, using an integrated analysis of the genotypic and phenotypic characters. our experimental methods on two genotypic characters, i.e., dna base composition using the hplc method and dna relatedness using the nitrocellulose filter hybridization technique between a. flavus, a. oryzae, and their close relations revealed a good agreement with the values by buoyant density (for dna base compo ...199912501399
antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery investigate the volatile fractions of 16 essential oils for activity against the more common fungi causing spoilage of bakery products, eurotium amstelodami, e. herbariorum, e. repens, e. rubrum, aspergillus flavus, a. niger and penicillium corylophilum.200312694455
metabolic products of fungi. ii. metabolic products of aspergillus amstelodami (mangin) thom et church. 195313155128
[a sector variation with proliferating sterigma of aspergillus amstelodami (mangin) thom and church]. 196213869985
[studies on the morphology and physiology of sterigma proliferation in aspergillus amstelodami (mangin) thom and church]. 196213869986
antibiotics from aspergillus amstelodami. 196314121196
modified atmosphere packaging for prevention of mold spoilage of bakery products with different ph and water activity levels.a sponge cake analog was used to study the influence of ph, water activity (aw), and carbon dioxide (co2) levels on the growth of seven fungal species commonly causing bakery product spoilage (eurotium amstelodami, eurotium herbariorum, eurotium repens, eurotium rubrum, aspergillus niger, aspergillus flavus, and penicillium corylophilum). a full factorial design was used. water activity, co2, and their interaction were the main factors significantly affecting fungal growth. water activity at lev ...200314572225
microbiological evolution of hay and relapse in patients with farmer's lung.recent studies in france have shown that absidia corymbifera and, to a lesser degree eurotium amstelodami and wallemia sebi, play a role in farmer's lung disease (fld), but that saccharopolyspora rectivirgula, classically incriminated, does not. little is known about farmers' reactions to these fungi or the circumstances which lead to exposure.200414691284
microbial exposure and mold-specific serum igg levels among children with respiratory symptoms in 2 school this study, the authors determined the association between serum mold-specific immunoglobulin g (igg) levels in primary school children (n = 181) with asthma, wheezing, or cough symptoms and exposure to indoor mold in 2 schools, with and without mold damage. microbial exposure was determined with environmental sampling. serum igg antibody concentrations against 24 microbial strains were determined with enzyme-linked immunosorbent assay. children's microbe-specific igg levels were often higher ...200314738273
early detection of fungal growth in bakery products by use of an electronic nose based on mass spectrometry.this paper presents the design, optimization, and evaluation of a mass spectrometry-based electronic nose (ms e-nose) for early detection of unwanted fungal growth in bakery products. seven fungal species (aspergillus flavus, aspergillus niger, eurotium amstelodami, eurotium herbariorum, eurotium rubrum, eurotium repens, and penicillium corylophillum) were isolated from bakery products and used for the study. two sampling headspace techniques were tested: static headspace (sh) and solid-phase mi ...200415453668
evaluation of mycological contamination of dental unit waterlines.the quality of dental unit water is of great importance since patients and dental staff are regularly exposed to water from aerosols generated during work. the main purpose of this investigation was mycological evaluation of dental unit waterlines (duwl). the author determined the number and species of fungi present in the water from a unit reservoir which is the source of water for a dental unit, in the water flowing from a high-speed handpiece of a unit, and in the swab sample collected from t ...200516028882
farmer's lung disease and microbiological composition of hay: a case-control study.previous studies performed in france have suggested that handling hay contaminated with high amounts of moulds, and especially absidia corymbifera and eurotium amstelodami, may favour farmer's lung disease. the circumstances favouring farmer's lung disease and the distinctive microbiological composition of hay samples that provoke attacks need to be specified. we present a case-control study which investigates the agricultural practices and the microbiological composition of hay handled in patie ...200516244894
evaluation of chemicals for restricting colony spreading by a xerophilic mold, eurotium amstelodami, on dichloran-18% glycerol agar.twenty chemicals were screened for their effectiveness in restricting colony spreading by four strains of a xerophilic mold, eurotium amstelodami, on dichloran-18% glycerol agar. triton x-100, triton x-301, tergitol np-7, and tergitol 15-s-3 (each at 200 mug/ml) and 1,000 mug of sodium deoxycholate, 1 mug of iprodione, 0.1 mug of propiconazole, and 0.01 mug of maxim per ml were judged to be most effective for restricting the rate of colony spreading.199216348725
microbial transformation of artemisinin to 5-hydroxyartemisinin by eurotium amstelodami and aspergillus niger.transformation of the anti-malarial drug artemisinin by the fungi eurotium amstelodami and aspergillus niger were investigated. cultures were grown in sucrose/malt broth with artemisinin for 14 days and extracted with ethyl acetate. extracts were characterized by liquid chromatography. two metabolites from each fungal extract were isolated and identified using mass spectrometry and nuclear magnetic resonance. 5beta-hydroxyartemisinin and 7beta-hydroxyartemisinin were isolated in 63 and 32% yield ...200616389544
evaluation of salting as a hay preservative against farmer's lung disease agents.salting is a traditional, empirical practice used commonly in dairy farming regions to prevent moulding and heating in hay. our aim was to evaluate the effect of salting hay on the proliferation of microorganisms, particularly thermophilic actinomycetes and moulds involved in farmer's lung disease. fifty-one pairs of salted and unsalted hay bales from 14 farms were produced during the haymaking season between march and july. both the salted and the unsalted bales came from the same field, and we ...200516457476
[study on the quantitative and qualitative composition of fungi in dried medicinal plants].the quantitative and qualitative composition of fungi was determined in selected dried medicinal plants purchased in one of the herbal shops in szczecin, poland. the samples examined were as follows: chamomile (flos chamomillae), peppermint (folium menthae piperitae), lemon balm (folium melissae), st. john's wort (herba hyperici), and two herbal mixtures. the fungal composition depended on the specified sample. xerophilic fungi, i.e. eurotium amstelodami, e. herbariorum, e. rubrum and wallemia s ...200516610669
effects of fungal interaction on ochratoxin a production by a. carbonarius at different temperatures and a(w).ochratoxin a is a well-known mycotoxin produced by species of the genera penicillium and aspergillus. ota-producing species from a. section nigri are considered the source of ota detected in grapes, dried vine fruits and wines. other fungi present in grapes during their maturation can grow and interact with ota-producing aspergillus species and affect ota production. in this study seven fungi (alternaria alternata, cladosporium herbarum, eurotium amstelodami, trichoderma harzianum, penicillium d ...200616735071
studies on the interaction between grape-associated filamentous fungi on a synthetic medium.eleven fungi isolated from grapes and sun-dried grapes (alternaria alternata, cladosporium herbarum, eurotium amstelodami, penicillium janthinellum, p. decumbens, trichoderma harzianum, candida sp., aspergillus carbonarius ota-negative, a. carbonarius ota-positive, a. niger var. niger. and a. japonicus var. aculeatus), were grown in snm medium at different water activities (0.82-0.97) and temperature (20-40 degrees c) levels for 18 days. pairs of one aspergillus faced with one non-aspergillus we ...200716996158
diagnostic value of serum precipitins to mould antigens in active hypersensitivity pneumonitis.serum precipitins have a controversial diagnostic value in hypersensitivity pneumonitis (hp). the present authors' objective was to assess their diagnostic value by developing scores from a panel of specific antigens tested by two techniques (electrosyneresis and double diffusion) to discriminate active hp from other interstitial lung diseases. consecutive patients presenting with a condition for which hp was considered in the differential diagnosis were included in the study. all patients under ...200717182654
effect of intra and interspecific interaction on ota production by a. section nigri in grapes during dehydration.the aim of this study was to assess the colonizing capability and ota production of different populations of aspergillus section nigri spp. in grapes, as affected by the interactions with other fungi, during a simulated in vitro sun-drying. mature white grapes were divided into two lots of healthy and artificially injured grapes and inoculated with a. section nigri spp. (a. carbonarius ota producer, a. niger aggregate ota producer, a. niger aggregate ota non-producer), eurotium amstelodami and p ...200717188203
antifungal efficacy of hydrogen peroxide in dental unit waterline disinfection.the concentration and composition of fungal flora in dental unit waterlines (duwl) were evaluated. for this purpose, water samples from unit reservoirs and high-speed handpieces, and biofilm samples from the waterline walls from units were collected. subsequently, analogous samples from duwl were taken before and after disinfection using agent containing hydrogen peroxide. in the examined samples, the yeast-like fungi candida albicans and candida curvata were found. the following species of moul ...200617196007
effect of preharvest fungicides and interacting fungi on aspergillus carbonarius growth and ochratoxin a synthesis in dehydrating evaluate the effect of preharvest grape pesticides in aspergillus section nigri infection in dehydrating grapes and the final ochratoxin a (ota) content. additionally, the effect of coinoculation of moulds frequently isolated from grapes and raisins on aspergillus section nigri infection was studied.200717651218
assessment of four serological techniques in the immunological diagnosis of farmers' lung' lung disease (fld) is a pulmonary disease that results from repeated inhalation of antigens from mouldy hay or straw. the objective of this prospective study was to assess the reliability of four serological techniques in fld diagnosis. sera from 15 consecutive patients with fld, 15 healthy control farmers and 30 urban controls were analysed using four serological techniques [electrosyneresis (es), ouchterlony double diffusion (dd), elisa and western blot (wb)] with four antigens (absid ...200717893167
characterization of fungi in chronic rhinosinusitis using polymerase chain reaction and sequencing.the role of fungi in chronic rhinosinusitis (crs) remains unknown. fungi were also determined as one of the responsible agents in the etio-pathogenesis, while several studies found fungi in 6-93% of the cases. the aim of this study is to test the presence of fungi in samples taken from the middle meatus of patients with crs, using traditional culture methods and polymerase chain reaction (pcr), and to compare the efficacy of these methods. thirty patients diagnosed with crs, with or without nasa ...200818026743
moulds and ochratoxin a on surfaces of artisanal and industrial dry sausages.the use of moulds as a seasoning for sausage can have both desirable and undesirable consequences. the desirable consequences are the creation of a successful product that appeals to consumers. the undesirable consequences are due to the growth of undesirable moulds that produce highly toxic secondary metabolites referred to as mycotoxins. the aim of the paper was to investigate the presence of moulds producing ochratoxin a (ota) on the surface of sausages from northern italy. a total of 757 mou ...200919028307
evaluation of quantitative pcr and culture methods for detection of house dust fungi and streptomycetes in relation to moisture damage of the house.microbial concentrations in vacuumed house dust samples (n = 71) were analysed by culture and quantitative polymerase chain reaction (qpcr) methods and their association with extent of moisture damage in the house was studied.200819241524
comparison of three antigenic extracts of eurotium amstelodami in serological diagnosis of farmer's lung france and finland, farmer's lung disease (fld), a hypersensitivity pneumonitis common in agricultural areas, is mainly caused by eurotium species. the presence of antibodies in patients' serum is an important criterion for diagnosis. our study aimed to improve the serological diagnosis of fld by using common fungal particles that pollute the farm environment as antigens. fungal particles of the eurotium species were observed in handled hay. a strain of eurotium amstelodami was grown in vitro ...201019906892
new evidence of the involvement of lichtheimia corymbifera in farmer's lung disease.farmer's lung disease (fld) is a form of hypersensitivity pneumonitis resulting from recurrent exposure to moldy plant materials. we investigated and compared the initial response of respiratory epithelium after exposure to extracts of sacharopolyspora rectivirgula, lichtheimia corymbifera (formerly absidia corymbifera), eurotium amstelodami and wallemia sebi. the two criteria for selection of these species were their high prevalence in the hay handled by fld patients and the presence of high le ...201020353311
experimental induction of recurrent airway obstruction with inhaled fungal spores, lipopolysaccharide, and silica microspheres in evaluate experimental induction of recurrent airway obstruction (rao) with inhaled fungal spores, lipopolysaccharide, and silica microspheres in horses.201020513185
surface mycobiota on argentinean dry fermented sausages.the diversity of the mycobiota community occurring on the surface of fermented dry sausages and in the environment of the processing plants was studied. the manufacturing plants (five in total) were located in three different areas of southern buenos aires province (argentina). samples were collected from two types of fermented sausages (short and long ripening time) at two times of the year (winter and summer) and at different types of plants (artisanal and industrial). a total of 342 samples w ...201020643490
effects of ozone exposure on the xerophilic fungus, eurotium amstelodami is-sab-01, isolated from naan bread.xerophilic moulds cause contamination and spoilage of low moisture foods. this study examined the effect of ozone fumigation on growth of a eurotium species isolated from naan bread. two ozone treatments were used - a low-level long-term exposure (0.4 μmol/mol for 21 days) and high-level short-term exposure (300 μmol/mol for 5 to 120 min). for the low level exposure the combination of different media sucrose concentrations (0, 5, 10 and 20% w/v) with ozone treatment was also assessed. the growth ...201021093088
hypersaline waters - a potential source of foodborne toxigenic aspergilli and penicillia.previous studies of hypersaline environments have revealed the dominant presence of melanized yeast-like fungi and related cladosporium spp. in this study, we focused on the genera aspergillus and penicillium and their teleomorphic forms. from oligotrophic and eutrophic hypersaline waters around the world, 60 different species were identified, according to their morphological characteristics and extrolite profiles. for the confirmation of five new species, additionally, sequence analysis of the ...201121477006
taxonomy of eurotium species isolated from meju.eurotium strains were isolated from 77 loaves of meju (dried fermented soybeans), in various regions of korea from 2008 to 2010. morphological characteristics and dna sequences of β-tubulin were examined. they were identified as eurotium amstelodami, e. chevalieri, e. herbariorum, e. repens, e. rubrum, and e. tonophilum. of these species, e. chevalieri and e. tonophilum had not been previously reported in association with meju. e. chevalieri and e. repens were the species isolated most frequentl ...201121887653
variable beta-glucans production by different states of eurotium amstelodami explains differences in inflammatory responses in airway cells.eurotium amstelodami, a mold frequently identified in housing and farm air samples, is a suspected cause of respiratory diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome. this fungus is present in the air in three different states (ascospores, conidia, and hyphae). the aim of this study was to test in vitro the differential inflammatory response of airway cells exposed to 1,3 betaglucanase-treated protein extract (bgpe), from e. amstelodami ascospores, conidia, ...201121851418
fret-based quantum dot immunoassay for rapid and sensitive detection of aspergillus this study, a fluorescence resonance energy transfer (fret)-based quantum dot (qd) immunoassay for detection and identification of aspergillus amstelodami was developed. biosensors were formed by conjugating qds to igg antibodies and incubating with quencher-labeled analytes; qd energy was transferred to the quencher species through fret, resulting in diminished fluorescence from the qd donor. during a detection event, quencher-labeled analytes are displaced by higher affinity target analytes ...201122163961
pcr-sscp-based reconstruction of the original fungal flora of heat-processed meat processing of spoiled meat is prohibited by law, since it is a deception and does not comply with food safety aspects. in general, spoilage of meat is mostly caused by bacteria. however, a high contamination level of fungi could be also found in some meat or meat products with certain preserving conditions. in case that unhygienic meat is used to produce heat processed products, the microorganisms will be deactivated by heat, so that they cannot be detected by a standard cultivation method. ...201323361099
an impedance-based mold sensor with on-chip optical reference.a new miniaturized sensor system with an internal optical reference for the detection of mold growth is presented. the sensor chip comprises a reaction chamber provided with a culture medium that promotes the growth of mold species from mold spores. the mold detection is performed by measuring impedance changes with integrated electrodes fabricated inside the reaction chamber. the impedance change in the culture medium is caused by shifts in the ph (i.e., from 5.5 to 8) as the mold grows. in ord ...201627690039
glycerol enhances fungal germination at the water-activity limit for life.for the most-extreme fungal xerophiles, metabolic activity and cell division typically halts between 0.700 and 0.640 water activity (approximately 70.0-64.0% relative humidity). here, we investigate whether glycerol can enhance xerophile germination under acute water-activity regimes, using an experimental system which represents the biophysical limit of earth's biosphere. spores from a variety of species, including aspergillus penicillioides, eurotium halophilicum, xerochrysium xerophilum (form ...201727631633
antifungal properties of fermentates and their potential to replace sorbate and propionate in pound cake.the major objective of this study was to assess the antifungal activities of commercially available 'clean label' fermentates and their potential to replace the preservative function of sorbate and propionate in cake. this study was performed in two parts. in the first part of the study the inhibitory activities of selected fermentates - fa, fb, fc and fd - towards aspergillus tritici and aspergillus amstelodami were assessed as a function of ph (5.0-6.5) on malt extract agar (mea). in the secon ...201627574008
immunological consequences of intestinal fungal dysbiosis.compared to bacteria, the role of fungi within the intestinal microbiota is poorly understood. in this study we investigated whether the presence of a "healthy" fungal community in the gut is important for modulating immune function. prolonged oral treatment of mice with antifungal drugs resulted in increased disease severity in acute and chronic models of colitis, and also exacerbated the development of allergic airway disease. microbiota profiling revealed restructuring of fungal and bacterial ...201627237365
evaluation of the risk of fungal spoilage when substituting sucrose with commercial purified stevia glycosides in sweetened bakery products.the objectives of this study were to compare the effect of different stevia-based sugar substitutes (s1-s3), sucrose alone and a mixture of sucrose+s1 on: (a) humectant properties, (b) relative colonisation rates of sponge cake slices at 0.90 aw by strains of aspergillus flavus, eurotium amstelodami, fusarium graminearum and penicillium verrucosum at 20 and 25°c and (c) shelf-life periods in days prior to visible growth. results showed that sucrose, s1 commercial sugar substitute and the mixture ...201627183230
positive fungal quantitative pcr and th17 cytokine detection in bronchoalveolar lavage fluids: complementary biomarkers of hypersensitivity pneumonitis?interstitial lung disease (ild) is a large group of diseases, including hypersensitivity pneumonitis (hp), idiopathic pulmonary fibrosis (ipf) and sarcoidosis.201627098083
control of household mycoflora in fermented sausages using phenolic fractions from olive mill wastewaters.biopreservation using polyphenols represents an alternative to chemical molecules for improving food safety. in this work, we evaluated the antifungal activity of polyphenols extracted from olive mill wastewater (omwwp) to reduce or eliminate the growth of undesired fungi on the surface of dry fermented sausages. antagonism against penicillium expansum dsmz 1282, penicillium verrucosum dsmz 12639, penicillium nalgiovense ms01, aspergillus ochraceus dsmz 63304, cladosporium cladosporioides ms12, ...201525996624
chaophilic or chaotolerant fungi: a new category of extremophiles?it is well known that few halophilic bacteria and archaea as well as certain fungi can grow at the highest concentrations of nacl. however, data about possible life at extremely high concentrations of various others kosmotropic (stabilizing; like nacl, kcl, and mgso4) and chaotropic (destabilizing) salts (nabr, mgcl2, and cacl2) are scarce for prokaryotes and almost absent for the eukaryotic domain including fungi. fungi from diverse (extreme) environments were tested for their ability to grow a ...201425566222
survey of bovine mycotic mastitis in different mammary gland statuses in two north-eastern regions of algeria.the aim of this study was to evaluate the prevalence of mycotic mastitis in different mammary gland statuses. the study was conducted on 304 dairy cows from ten farms in two north-eastern regions in algeria; guelma and souk ahras with 922 and 199 samples, respectively, forming thus a total number of 1,121 milk samples. a total of 321 milk samples were collected from clinical mastitis, 544 milk samples from subclinical mastitis and 256 milk samples from healthy mammary glands. mycological analyse ...201525481847
identification and characterisation of organisms associated with chocolate pralines and sugar syrups used for their production.spoilage of chocolate pralines, due to growth of microorganisms tolerating low water activity, causes problems in the confectionary industry. therefore, an increased knowledge on which organisms are present in the chocolate fillings and their tolerance towards low aw, ph, ethanol and other preservatives is needed. using media containing 40-50% glucose (aw 0.872-0.925) bacteria, yeasts and moulds were isolated from chocolate pralines (aw 0.70-0.898) of nine manufactures and sugar syrups (aw 0.854 ...201424997272
anti-inflammatory activity of questinol isolated from marine-derived fungus eurotium amstelodami in lipopolysaccharide-stimulated raw 264.7 the present study, an anthraquinone derivative, questinol was successfully isolated from the broth extract of the marine-derived fungus eurotium amstelodami for the first time. the structure of questinol was determined based on the analysis of the ms and nmr spectral data as well as comparison of those data with the published data. moreover, the anti-inflammatory effect of questinol in lipopolysaccharide (lps)-stimulated raw 264.7 cells was investigated. the results showed that questinol did ...201424986678
does fungicide application in vineyards induce resistance to medical azoles in aspergillus species?this study assessed if the use of sterol demethylase inhibitor fungicides in vineyard production can induce resistance to azoles in aspergillus strains and if it can induce selection of resistant species. we also tried to identify the aspergillus species most prevalent in the vineyards. two vineyards from northern portugal were selected from "vinhos verdes" and "douro" regions. the vineyards were divided into plots that were treated or not with penconazole (pen). in each vineyard, air, soil, and ...201424833021
evaluation of the conjunctival fungal flora and its susceptibility to antifungal agents in healthy horses in characterize the conjunctival fungal flora and to determine the susceptibility of 2 isolated molds to antifungal drugs in samples of 64 healthy horses from the national stud in switzerland.201423910390
human monocyte-derived dendritic cells exposed to microorganisms involved in hypersensitivity pneumonitis induce a th1-polarized immune response.hypersensitivity pneumonitis (hp) is an immunoallergic disease characterized by a prominent interstitial infiltrate composed predominantly of lymphocytes secreting inflammatory cytokines. dendritic cells (dcs) are known to play a pivotal role in the lymphocytic response. however, their cross talk with microorganisms that cause hp has yet to be elucidated. this study aimed to investigate the initial interactions between human monocyte-derived dcs (modcs) and four microorganisms that are different ...201323720369
ulcerative fungal keratitis in a brown swiss 11-year-old brown swiss cow was referred to the farm animal department of the veterinary teaching hospital in zurich, switzerland, because of lateral recumbency due to puerperal hemolytic anemia. the animal had developed enophthalmos due to dehydration at the time of presentation. two days after hospitalization, the cow showed blepharospasm and epiphora of the right eye. ophthalmic examination of the right eye revealed a fluorescein-positive, paraxial, superficial corneal ulcer with focal ede ...201323437914
identification of volatile markers for indoor fungal growth and chemotaxonomic classification of aspergillus species.microbial volatile organic compounds (mvocs) were collected in water-damaged buildings to evaluate their use as possible indicators of indoor fungal growth. fungal species isolated from contaminated buildings were screened for mvoc production on malt extract agar by means of headspace solid-phase microextraction followed by gas chromatography-mass spectrometry (gc-ms) analysis. some sesquiterpenes, specifically derived from fungal growth, were detected in the sampled environments and the corresp ...201222954337
[farmer's lung antigens in germany].recent studies suggest that besides the long-known farmer's lung antigen sources saccharopolyspora rectivirgula (micropolyspora faeni), thermoactinomyces vulgaris, and aspergillus fumigatus, additionally the mold absidia (lichtheimia) corymbifera as well as the bacteria erwinia herbicola (pantoea agglomerans) and streptomyces albus may cause farmer's lung in germany. in this study the sera of 64 farmers with a suspicion of farmer's lung were examined for the following further antigens: wallemia ...201222477566
anatomy of amplified mitochondrial dna in "ragged" mutants of aspergillus amstelodami: excision points within protein genes and a common 215 bp segment containing a possible origin of replication.the extranuclearly-inherited ragged growth phenotype (rgd) of aspergillus amstelodami is always accompanied by excision and head-to-tail amplification of mtdna sequences. in one mutant strain (rgd1) the amplified mtdna segment (rgd1 dna, monomeric length 0.9 kb) maps downstream of the large subunit ribosomal rna gene (region 1), whereas in all other strains analyzed the amplified sequences (rdg3-7dna) are located in region 2 between genes coding for cytochrome b and atpase subunit 6. the various ...198124185955
analyses of fungal community by illumina miseq platforms and characterization of eurotium species on liupao tea, a distinctive post-fermented tea from china.liupao tea is a distinctive chinese dark tea obtained by indigenous tea fermentation facilitated by the symbiotic association of bacteria and fungi. the composition of fungal community in 4 liupao tea samples stored for several years under natural microbial fermentation was evaluated by miseq sequencing. taxonomic analysis revealed 3 phyla, 6 families, 8 genera. the genera eurotium and aspergillus were dominant fungi in almost all the samples. a total of 85 strains found in 41 other tea samples ...201728784527
characterization of the translation elongation factor 1-α gene in a wide range of pathogenic aspergillus species.we aimed to evaluate the resolving power of the translation elongation factor (tef)-1α gene for phylogenetic analysis of aspergillus species.201728425876
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