| the nucleotide sequence of the 5s ribosomal rna gene of pyrenophora graminea. | | 1991 | 1923773 |
| genomic regions determining resistance to leaf stripe (pyrenophora graminea) in barley. | leaf stripe is a seed-borne disease of barley (hordeum vulgare) caused by pyrenophora graminea. little is known about the genetics of resistance to this pathogen. in the present work, qtl analysis was applied on two recombinant inbred line (ril) populations derived from two- and six-rowed barley genotypes with different levels of partial resistance to barley leaf stripe. quantitative trait loci for partial resistance were identified using the composite interval mapping (cim) method of plabqtl so ... | 2002 | 12033613 |
| gypsy-like retrotransposons in pyrenophora: an abundant and informative class of molecular markers. | this paper describes the development of s-sap (sequence-specific amplified polymorphism) using a primer derived from the ltr (long terminal repeat) of the pyggy retrotransposon isolated from pyrenophora graminea. fragments were amplified by s-sap from different pyrenophora spp., indicating the presence of pyggy-like sequences in these genomes. the bands were highly polymorphic between isolates and the number of bands differed by as much as 10-fold between species, demonstrating the potential of ... | 2004 | 15190369 |
| in vitro antifungal activity of the tea tree (melaleuca alternifolia) essential oil and its major components against plant pathogens. | the aim of this study was to examine the effect of melaleuca alternifolia essential oil (tto) and its principal components on four cereal-pathogenic fungi. | 2007 | 17576222 |
| the application of real-time pcr to the identification, detection and quantification of pyrenophora species in barley seed. | summary a real-time quantitative pcr technique has been used to develop a rapid and sensitive seed health test for pyrenophora spp. on barley seed. using the fluorescent reporter dye sybr green i for real-time detection of pcr amplification, pathogen dna extracted from infected seed can be quantified to the picogram level. the amount of pyrenophora dna extracted from seed samples of an artificial infection level gradient, constructed by mixing infected and uninfected seed, correlated with good a ... | 2001 | 20572991 |
| efficiency of irap and its-rflp marker systems in accessing genetic variation of pyrenophora graminea. | the usefulness of irap (inter-retrotransposon amplified polymorphism) and its-rflp (restriction of pcr-amplified internal transcribed spacers of the rdna) markers in the analysis of 39 pyrenophora graminea isolates was determined. each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. irap and its-rflp produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (pic) of 0.38 ... | 2010 | 21637490 |
| pcr amplification and characterization of the intergenic spacer region of the ribosomal dna in pyrenophora graminea. | successful amplification of the whole intergenic spacer region of the nuclear ribosomal repeat (igs) in pyrenophora graminea was obtained with a pcr-based assay. single amplification products showed length differences. depending on the length of the igs-pcr product, ca. 3.8 or 4.4 kb, two groups of isolates could be identified. the rflp patterns of isolates obtained with the 6-base cutting enzymes apal, bglii, drai, ecorv, hindiii and saci were similar within each group and different between the ... | 1998 | 9741081 |
| Molecular and xylanolytic variation identified among strains of Pyrenophora graminea. | The inter-retrotransposon amplified polymorphism (IRAP) was used to confirm the genetic variation among 22 strains of Pyrenophora graminea differing in their xylanase production. A total of 162 bands were scored of which 151 (93.21%) were polymorphic. The molecular parameter used showed that P. graminea strains reside in four phylogenetic groups. There was observed the resolution between clustering strains and their xylanase production. Hence, the described approach presented here constitutes no ... | 2011 | 21861376 |
| Heterogeneity in the ITS of the ribosomal DNA of Pyrenophora graminea isolates differing in xylanase and amylase production. | Xylanase and amylase have gained increasing interest because of their various biotechnology applications. In this research, the restriction of PCR-amplified internal transcribed spacers (ITS) of ribosomal DNA (rDNA) was used to confirm the genetic variation among 22 isolates of Pyrenophora graminea differing in their xylanase and amylase production. The fingerprints generated from the six restriction digestions of the rDNA ITS region showed high levels ofintraspecific variation within the P. gra ... | 2011 | 22073549 |
| the cc-nb-lrr-type rdg2a resistance gene confers immunity to the seed-borne barley leaf stripe pathogen in the absence of hypersensitive cell death. | leaf stripe disease on barley (hordeum vulgare) is caused by the seed-transmitted hemi-biotrophic fungus pyrenophora graminea. race-specific resistance to leaf stripe is controlled by two known rdg (resistance to drechslera graminea) genes: the h. spontaneum-derived rdg1a and rdg2a, identified in h. vulgare. the aim of the present work was to isolate the rdg2a leaf stripe resistance gene, to characterize the rdg2a locus organization and evolution and to elucidate the histological bases of rdg2a- ... | 2010 | 20844752 |
| identification and mapping of the leaf stripe resistance gene rdg1a in hordeum spontaneum. | leaf stripe of barley, caused by pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown nordic and mediterranean barley districts. two barley segregating populations represented by 103 recombinant inbred lines (rils) of the cross l94 (susceptible) x vada (resistant) and 194 rils of the cross arta (susceptible) x hordeum spontaneum 41-1 (resistant) were analysed with two highly virulent leaf stripe isolates, dg2 and dg5, to identify loci f ... | 2010 | 20041226 |
| histological and molecular analysis of rdg2a barley resistance to leaf stripe. | barley (hordeum vulgare l.) leaf stripe is caused by the seed-borne fungus pyrenophora graminea. we investigated microscopically and molecularly the reaction of barley embryos to leaf stripe inoculation. in the resistant genotype nil3876-rdg2a, fungal growth ceased at the scutellar node of the embryo, while in the susceptible near-isogenic line (nil) mirco-rdg2a fungal growth continued past the scutellar node and into the embryo. pathogen-challenged embryos of resistant and susceptible nils show ... | 2008 | 18705861 |
| phylogeny and evolution of mating-type genes from pyrenophora teres, the causal agent of barley "net blotch" disease. | the main aim of this study was to test the patterns of sequence divergence and haplotype structure at the mat locus of pyrenophora teres, the causal agent of barley 'net blotch' disease. p. teres is a heterothallic ascomycete that co-occurs in two symptomatological forms, the net form (nf) and the spot form (sf). the mating-type genes mat1-1-1 and mat1-2-1 were sequenced from 22 nf isolates (12 mat1-1-1 and 10 mat1-2-1 sequences) and 17 sf isolates (10 mat1-1-1 and seven mat1-2-1 sequences) coll ... | 2007 | 17426975 |
| high-resolution genetic mapping of the leaf stripe resistance gene rdg2a in barley. | the dominant gene rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen pyrenophora graminea is located in the distal region of chromosome arm 1 (7h)s. as the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an f(2) population of 1,400 plants (thibaut rdg2axmirco). the map included six classes of resistance gene analogues (rgas) tightly associated with rdg2a. rdg2a was delimited to a genetic interval of 0.14 cm be ... | 2004 | 14689188 |
| barley leaf stripe disease. | leaf stripe is one of the most important diseases of barley in iran especially in gorgan, mazandran and near tehran (varamin). most obvious symptoms of the disease are described. long pale or yellow stripes become darker as the fungus sporulates on the leaf surface. infected plants usually are stunted and produce sterile spikes, rarely a few seeds are produced. infected spikes and late-forming tillers may produce fertile spikes. the fungus is seed brone and survives in the outer layers of infect ... | 2002 | 12701433 |
| isolate-specific qtls of resistance to leaf stripe (pyrenophora graminea) in the 'steptoe' x 'morex' spring barley cross. | leaf stripe caused by the fungus pyrenophora graminea represents a serious threat to grain yield in organically grown barley and in conventional nordic and mediterranean districts, for which resistant cultivars are necessary. a medium-density, molecular marker map derived from a 'steptoe' (partially resistant) x 'morex' (susceptible) spring barley cross and its derived doubled-haploid mapping population inoculated with the fungus made it possible to identify qtls of resistance to leaf stripe. in ... | 2003 | 12595996 |