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properties of alkaline phosphatase of the halotolerant yeast debaryomyces hansenii.the molecular weight of a partially purified alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, ec 3.1.3.1) from the halotolerant yeast debaryomyces hansenii was estimated to 110,000 by gel filtration. the isoelectric point determined by electrofocusing was at approximately ph 4.4. the enzyme had a broad specificity against phosphomonoesters and also attacked some acid anhydrides. arsenate, molybdate, and orthophosphate acted as competitive inhibitors. various metal-binding agents ...1978413579
[the antigenic relationship between salmonella 0:7 antigen and debaromyces hansenii and its synonym strains].in this publication antigenic relationship between salmonella 0:7 antigen and debaryomyces marama, debaryomyces hansenii and some synonym strains of debaryomyces hansenii are shown together.1979555795
the antigenic relationship between brettanomyces-debaryomyces strains and the salmonella cholerae-suis o antigen.the immune sera for brettanomyces lambicus, b. claussenii, debaryomyces hansenii and d. marama agglutinated salmonella cholerae-suis (0:6(2), 7). the immune serum for s. cholerae-suis agglutinated b. lambicus, b. clausenni, d. hansenii and d. marama. absorption and agglutination cross-tested demonstrated common antigen factor(s) in the tested yeasts and salmonella 0:7 antigen.1978567026
ploidy, ascus formation and recombination in torulaspora (debaryomyces) hansenii.x-ray inactivation studies on the type strain of torulaspora hansenii carried out to determine ploidy, provided proof that the species has a haplontic life cycle, a fact which hitherto has only been presumed. observations on the genesis of the ascus by light microscopy and transmission electron microscopy provide no evidence for, what some earlier workers in this field have presumed to be, heterogamous conjugation between a mother cell and its bud. they do, however, show that asci, bearing obliq ...1977596863
[candida mogii and debaryomyces hansenii yeast development in the presence of metal chlorides].the yeasts candida mogii 2 and debaryomyces hansenii 8 isolated from salted fish spawn in the process of its storage were found to be capable of growth in 4 m kcl, 3 m mgcl2, 2.5 m nacl, 1.5 bacl2 and 1 m cacl2. the activity of water (aw) in these solutions varied from 0.983 to 0.719; the maximum osmotic pressure was 380 atm. the cultures grew also in a 3 m sucrose solution, at low concentrations of osmotically active substances and without them. therefore, they can be regarded as osmotolerant m ...1978651691
[lipolytic activity of osmotolerant yeasts].the ability to hydrolyse olive oil, cod-liver oil, caviar oil, and yeast oil was studied in osmotolerant yeasts candida mogii 2, debaryomyces hansenii 8, and rhodotorula rubra 9, isolated from salted caviar. the lipolytic activity was manifested at 5, 15 and 26 degrees c and ph 4.0--9.2, even in the presence of high concentrations (0.5--3 m) of such chlorides as kcl, nacl and cacl2. the lipolytic activity of s variant of d. hansenii 8 was higher than the activity of its r variant.1978661632
the effect of the water activity of the milieu on rates of glucose uptake by the osmophilic yeasts saccharomyces rouxii and debaryomyces hansenii.rates of glucose uptake in baker's yeast and in the osmophilic yeasts d. hansenii and s. rouxii were investigated at different values of water activity of the milieu, as regulated either by glycerol or sodium chloride. in both cases, d. hansenii could maintain relatively higher rates of glucose uptake. at lower values of water activity, sodium chloride exerted an inhibitory effect on rates of glucose uptake by s. rouxii, while in the presence of glycerol, rates of glucose uptake shown by s. roux ...1978749415
purification of alkaline phosphatase of the halotolerant yeast debaryomyces hansenii.alkaline phosphatase (orthophosphoric monoester phosphohydrolase, ec 3.1.3.1) of the halotolerant yeast debaryomyces hansenii was purified by a procedure involving cell disruption, dnaase treatment, ethanol precipitation, gel filtration, chromatography on deae-sephadex, and preparative polyacrylamide gel electrophoresis. the specific activity was increased 1250-fold as compared to the activity of cell free extract. the total recovery was 30%. various modifications of the growth conditions had sl ...1976948964
on the mechanism of salt tolerance. production of glycerol and heat during growth of debaryomyces hansenii.as glycerol was suggested as an osmotic agent in the salt tolerant debaryomyces hansenii the concentrations of total, intracellular, and extracellular glycerol produced by this yeast was followed during growth in 4 mm, 0.68 m, and 2.7 m nacl media. the total amount of glycerol was not directly proportional to biomass production but to the cultural salinity with maximum concentrations just prior to or at the beginning of the stationary phase. in all cultures the cells lost some glycerol to the me ...19761015945
electron microscopy of ascus formation in the yeast debaryomyces hansenii.ascus formation in debaryomyces hansenii includes fusion of two cells, usually mother and daughter while still attached to each other, through short protuberances developed from the cross wall between them. nuclear fusion takes place in the channel connecting the two cells; meiosis apparently occurs in the mother cell. generally, only one lobe of the meiotic nucleus is surrounded by a prospore wall and it becomes the nucleus of a spore with a warty wall. the rest of the nucleus disappears. the s ...19751176954
isolation and characterization of an autonomously replicating sequence (arsd) from the marine yeast debaryomyces hansenii.the marine yeast debaryomyces hansenii is known to tolerate salinities ranging from 0 to 24%. as a first step toward the molecular analysis of halotolerance in this organism, we report the isolation of an autonomously replicating sequence (ars) and its use in the construction of a shuttle vector. the ars from d. hansenii (arsd) is 0.4 kbp long, and the function rests in 0.13 kbp of the sequence. sequence analysis of arsd shows strong homology to ars from other organisms, including a 12-bp consen ...19921308205
study of surface yeast flora of roquefort cheese.the change in yeast flora on the surface of two batches of roquefort cheese was monitored over a period of 6 months. 401 isolates were determined and their technological properties were investigated. the main species isolated were: debaryomyces hansenii and its non sporulating form candida famata, kluyveromyces lactis and its non sporulating form candida sphaerica and candida species. the species debaryomyces hansenii inoculated on the surface of the cheese in one of the batches just before the ...19921476870
study of surface yeast flora of roquefort cheese.the change in yeast flora on the surface of two batches of roquefort cheese was monitored over a period of 6 months. 401 isolates were determined and their technological properties were investigated. the main species isolated were: debaryomyces hansenii and its non sporulating form candida famata, kluyveromyces lactis and its non sporulating form candida sphaerica and candida species. the species debaryomyces hansenii inoculated on the surface of the cheese in one of the batches just before the ...19921476870
isolation and sequence analysis of the small subunit ribosomal rna gene from the euryhaline yeast debaryomyces hansenii.the small subunit ribosomal rna gene (ssu rdna) from the euryhaline yeast debaryomyces hansenii has been isolated and sequenced. after appropriate alignment of this sequence with ssu rdna sequences from 30 other taxa, phylogenetic reconstruction using distance matrix and maximum parsimony methods indicates that d. hansenii is most closely affiliated with candida albicans, and occurs in the cluster of the yeasts saccharomyces cerevisiae, torulaspora delbruekii, candida glabrata, and kluyveromyces ...19921525872
fungal arthritis due to infection by candida famata in a horse. 19921586319
fungal arthritis due to infection by candida famata in a horse. 19921586319
torulopsis candida (candida famata) endophthalmitis simulating propionibacterium acnes syndrome.four months after undergoing extracapsular cataract extraction with implantation of a posterior chamber intraocular lens, a 74-year-old woman developed granulomatous anterior uveitis. although she initially responded well to corticosteroid therapy, she experienced multiple recurrences on discontinuation of this therapy. slit-lamp examination showed the ocular inflammation to be associated with white cortical material within the lens capsular sac. she underwent removal of the implant as well as t ...19911841584
torulopsis candida (candida famata) endophthalmitis simulating propionibacterium acnes syndrome.four months after undergoing extracapsular cataract extraction with implantation of a posterior chamber intraocular lens, a 74-year-old woman developed granulomatous anterior uveitis. although she initially responded well to corticosteroid therapy, she experienced multiple recurrences on discontinuation of this therapy. slit-lamp examination showed the ocular inflammation to be associated with white cortical material within the lens capsular sac. she underwent removal of the implant as well as t ...19911841584
silver tolerance and accumulation in yeasts.debaryomyces hansenii (ncyc 459 and strain 75-21), candida albicans (3153a), saccharomyces cerevisiae (x2180-1b), rhodotorula rubra (ncyc 797) and aureobasidium pullulans (imi 45533 and atcc 42371) were grown on solid medium supplemented with varying concentrations of agno3. although ag+ is highly toxic towards yeasts, growth on solid media was still possible at ag concentrations of 1-2 mm. further subculture on higher ag concentrations (up to 5 mm) resulted in elevated tolerance. the extent of ...19911878280
natural abundance 13c-nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress.analysis of seventeen yeast strains by 13c-nmr spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt-stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. ribitol was detected in some species, including debaryomyces hansenii, although ribitol accumulation did not correlate with the osmotic pressure of the medium. relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmo ...19911936945
purification and characterization of glycerol-3-phosphate dehydrogenase (nad+) in the salt-tolerant yeast debaryomyces hansenii.the nad-dependent glycerol-3-phosphate dehydrogenase (ec 1.1.1.8) of the salt-tolerant yeast debaryomyces hansenii was purified by poly(ethylene glycol) precipitation and a combination of chromatographic procedures. the enzyme existed in two forms with different ionic characters and specific activity. on sds-polyacrylamide gel electrophoresis, both forms yielded one predominant band with an apparent molecular weight of 42,000. the specific activity of the enzyme was dependent on the concentratio ...19901972335
the yeasts of cheese brines.a total of 365 yeasts were isolated from the brines of soft, semihard and hard cheeses from different manufacturers. identification was based on 131 characteristics, primarily employing a method with microtitration plates. most brines exhibited a characteristic yeast flora. the predominant strains proved to be mainly debaryomyces hansenii and candida versatilis. in a few brines trichosporon beigelii, c. rugosa, c. intermedia, kluyveromyces marxianus, saccharomyces sp. and c. tenuis/polymorpha we ...19902282287
osmoregulation of the salt-tolerant yeast debaryomyces hansenii grown in a chemostat at different salinities.the intracellular solute composition of the salt-tolerant yeast debaryomyces hansenii was studied in glucose-limited chemostat cultures at different concentrations of nacl (4 mm, 0.68 m, and 1.35 m). a strong positive correlation between the total intracellular polyol concentration (glycerol and arabinitol) and medium salinity was demonstrated. the intracellular polyol concentration was sufficient to balance about 75% of the osmotic pressure of the medium in cultures with 0.68 and 1.35 m nacl. t ...19902318802
yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops.the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ...19902321936
yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops.the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ...19902321936
a procedure for enrichment and isolation of mutants of the salt-tolerant yeast debaryomyces hansenii having altered glycerol metabolism.the salt-tolerant yeast debaryomyces hansenii produces and accumulates glycerol when subjected to salt stress, whereby the buoyant density of the cells is changed. this property allows for enrichment of mutants with altered glycerol metabolism by density gradient centrifugation. colonies derived from cells with rapidly changing density following an osmotic shock were screened for increased glycerol production by observing their ability to support growth of a glycerol-requiring strain of escheric ...19902379814
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil.samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ...19892750315
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil.samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ...19892750315
glycerol production in relation to the atp pool and heat production rate of the yeasts debaryomyces hansenii and saccharomyces cerevisiae during salt stress.changes in glycerol production and two parameters related to energy metabolism i.e. the heat production rate and the atp pool, were assayed during growth of saccharomyces cerevisiae and debaryomyces hansenii in 4 mm and 1.35 m nacl media. for both of the yeasts, the specific atp pool changed during the growth cycle and reached maximum values around 10 nmol per mg dry weight in both types of media. the levels of glycerol were markedly enhanced by high salinity. in the presence of 1.35 m nacl, d. ...19873304183
osmotic significance of glycerol accumulation in exponentially growing yeasts.natural-abundance 13c-nuclear magnetic resonance spectroscopy has shown glycerol to be the major osmotically significant low-molecular-weight solute in exponentially growing, salt-stressed cells of the yeasts saccharomyces cerevisiae, zygosaccharomyces rouxii, and debaromyces hansenii. measurement of the intracellular nonosmotic volume (i.e., the fraction of the cell that is osmotically unresponsive) by using the boyle-van't hoff relationship (for nonturgid cells, the osmotic volume is directly ...19873314706
torulopsis candida, a new opportunistic pathogen.we described the first documented case of intravenous-catheter-associated fungemia caused by torulopsis candida (candida famata). the microorganism was isolated from two blood cultures and one intravenous catheter tip in a bone marrow transplant patient. both the intravascular cannula and the immunological status of the patient are believed to have played major roles in predisposing the patient to such an infection. uneventful recovery occurred after the removal of the catheter and amphotericin ...19863533991
torulopsis candida, a new opportunistic pathogen.we described the first documented case of intravenous-catheter-associated fungemia caused by torulopsis candida (candida famata). the microorganism was isolated from two blood cultures and one intravenous catheter tip in a bone marrow transplant patient. both the intravascular cannula and the immunological status of the patient are believed to have played major roles in predisposing the patient to such an infection. uneventful recovery occurred after the removal of the catheter and amphotericin ...19863533991
oxidation of amines by yeasts grown on 1-aminoalkanes or putrescine as the sole source of carbon, nitrogen and energy.the maximum growth rate of trichosporon cutaneum cbs 8111 in chemostat cultures was 0.185 h-1 on ethylamine and 0.21 h-1 on butylamine, that of candida famata cbs 8109 was 0.32 h-1 on putrescine. the amine oxidation pattern of the ascomycetous strains studied, viz. candida famata cbs 8109, stephanoascus ciferrii cbs 4856 and trichosporon adeninovorans cbs 8244 was independent of the amine that had been used as the growth substrate. it resembled that of benzylamine/putrescine oxidase found in oth ...19863813525
oxidation of amines by yeasts grown on 1-aminoalkanes or putrescine as the sole source of carbon, nitrogen and energy.the maximum growth rate of trichosporon cutaneum cbs 8111 in chemostat cultures was 0.185 h-1 on ethylamine and 0.21 h-1 on butylamine, that of candida famata cbs 8109 was 0.32 h-1 on putrescine. the amine oxidation pattern of the ascomycetous strains studied, viz. candida famata cbs 8109, stephanoascus ciferrii cbs 4856 and trichosporon adeninovorans cbs 8244 was independent of the amine that had been used as the growth substrate. it resembled that of benzylamine/putrescine oxidase found in oth ...19863813525
glycerol metabolism and osmoregulation in the salt-tolerant yeast debaryomyces hansenii.a glycerol-nonutilizing mutant of the salt-tolerant yeast debaryomyces hansenii was isolated. when subjected to salt stress the mutant produced glycerol, and the internal level of glycerol increased linearly in proportion to increases of external salinity as in the wild-type strain. however, at increased salinity the mutant showed a more pronounced decrease of growth rate and growth yield and lost more glycerol to the surrounding medium than did the wild type. uptake experiments showed glycerol ...19853980438
biogenesis and metabolic significance of microbodies in urate-utilizing yeasts.growth of candida famata and trichosporon cutaneum on uric acid as the sole source of carbon and nitrogen was associated with the development of a number of microbodies in the cells. cytochemical staining experiments showed that the organelles contained urate oxidase, a key enzyme of uric acid metabolism, and catalase. transfer of cells, precultured on glucose or glycerol, into uric acid-containing media indicated that these microbodies originated from the organelles, originally present in the i ...19854004201
biogenesis and metabolic significance of microbodies in urate-utilizing yeasts.growth of candida famata and trichosporon cutaneum on uric acid as the sole source of carbon and nitrogen was associated with the development of a number of microbodies in the cells. cytochemical staining experiments showed that the organelles contained urate oxidase, a key enzyme of uric acid metabolism, and catalase. transfer of cells, precultured on glucose or glycerol, into uric acid-containing media indicated that these microbodies originated from the organelles, originally present in the i ...19854004201
ultrastructure of debaryomyces hansenii. 19704193325
regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeasts.by using the isotope pairs (22)na-(24)na and (42)k-(86)rb, the uptake and retention of na and k was studied in the salt-tolerant debaryomyces hansenii and in the less tolerant saccharomyces cerevisiae at nacl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. the ratio of k to na is much higher in the cells than in the media, and higher in d. hansenii than in s. cerevisiae under comparable conditions. the difference between the two species is due to a better na extrusion and a better uptake ...19695354950
effects of topical anesthetics on pullalaria pullulans and debaryomyces hansenii.the inhibitory effects of three topical anesthetics of various concentrations on the growth of pullularia pullulans, debaryomyces hansenii, and on pigment production by p. pullulans were investigated. the topical anesthetics were benoxinate hydrochloride, proparacaine hydrochloride, and tetracaine hydrochloride. in decreasing order, the inhibiting effects of the drugs on growth were benoxinate, tetracaine, and proparacaine for p. pullulans, and tetracaine, benoxinate, and proparacaine for d. han ...19695392897
distribution of c-14 from glucose-1-c-14 in the lipid fractions of debaryomyces hansenii.merdinger, emanuel (roosevelt university, chicago, ill.), and rosalind h. frye. distribution of c(14) from glucose-1-c(14) in the lipid fractions of debaryomyces hansenii. j. bacteriol. 91:1831-1833. 1966.-debaryomyces hansenii cells were grown in a medium containing yeast extract, malt extract, glucose, sodium chloride, and nutrient salts, to which glucose-1-c(14) was added. the lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. of the total c( ...19665937239
antagonistic activity of debaryomyces hansenii towards clostridium tyrobutyricum and cl. butyricum.a study of the inhibitory action of debaryomyces hansenii (31 strains) on clostridium tyrobutyricum (5 strains) and cl. butyricum (2 strains) on laboratory media showed that deb. hansenii inhibited the growth of these organisms, and that this effect was due not only to competition for nutrients but also to the production of both extra- and intracellular antimicrobial metabolites. the inhibitory effect varied with strain and occurred whether the yeasts were grown aerobically or under reduced o2 t ...19836417206
the yeasts of british fresh sausage and minced beef.three hundred and eighty three yeasts isolated from samples of unsulphited or sulphited sausages and skinless sausages and minced beef were characterized in detail. debaryomyces hansenii was the most commonly isolated yeast from most samples followed by candida zeylanoides and pichia membranaefaciens. the presence of sulphite in sausages did not appear to affect the numbers and range of yeasts present but did affect their relative proportions. a survey of one factory showed that meat intended fo ...19846486769
growth of candida famata and trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts.yeast strains capable of utilizing uric acid as the sole source of carbon and energy were isolated from soil by the enrichment culture method. the strains were identified as candida famata (harrison) meyer et yarrow and trichosporon cutaneum (de beurm., gougerot et vaucher) ota. on the subcellular level growth of yeasts on uric acid was accompanied with the development of a number of large microbodies in the cells.19836651286
growth of candida famata and trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts.yeast strains capable of utilizing uric acid as the sole source of carbon and energy were isolated from soil by the enrichment culture method. the strains were identified as candida famata (harrison) meyer et yarrow and trichosporon cutaneum (de beurm., gougerot et vaucher) ota. on the subcellular level growth of yeasts on uric acid was accompanied with the development of a number of large microbodies in the cells.19836651286
[ascospore formation in yeasts related to torulopsis candida].ascospore formation was found in the type strains torulopsis famata (harrison) lodder et kreger van rij, t. minor (pollaci et nannizi) lodder and candida flareri (ciferri et redaelli) langeron et guerra. the names of these species should be considered as a synonym of debaryomyces hansenii (zopf) lodder et kreger van rij.19836686647
sensitivity of heat-stressed yeasts to essential oils of plants.eight strains of yeasts (candida lipolytica, debaryomyces hansenii, hansenula anomala, kloeckera apiculata, lodderomyces elongisporus, rhodotorula rubra, saccharomyces cerevisiae, and torulopsis glabrata) were examined for changes in sensitivity to eight essential oils of plants (allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme) after being sublethally heat stressed. with the exception of garlic oil for all test yeasts, onion oil for s. cerevisiae, and oregano oil for r. rubr ...19846712207
effect of nacl on kinetics of d-glucosamine uptake in yeasts differing in halotolerance.the initial rate of d-glucosamine uptake by the non-halotolerant yeast saccharomyces cerevisiae was approximately halved as the apparent half saturation constant (km) and the apparent maximum velocity (vmax) changed from 6.6 mm to 16.4 mm and from 22 mumol.g-1.min-1 to 16 mumol.g-1.min-1, respectively, when the salinity in the medium was increased from zero m to 0.68 m nacl. corresponding changes in a high affinity transport system in the halotolerant yeast debaryomyces hansenii were from 1.1 mm ...19817044305
bone infection caused by debaryomyces hansenii in a normal host: a case report.debaryomyces hansenii was isolated from a cystic lesion in the distal tibia of a healthy 23-year-old woman. ascospores were demonstrated when the organism was incubated at 25 but not at 30 degrees c. electron microscopy was necessary to demonstrate the warty surface of the ascospore wall. growth was inhibited in vitro by low concentrations of amphotericin b, 5-fluorocytosine, miconazole, and ketoconazole. four previous cases of infection caused by debaryomyces spp. and one caused by the related ...19827130364
organic growth factor requirements of some yeasts.some sporogenous yeasts (brettanomyces bruxellensis, debaryomyces hansenii, hansenula ciferrii, hansenula polymorpha, pichia polymorpha, saccharomycopsis guttulata, and saccharomyces chevalieri), isolated from various fruits have been examined for their organic growth factor requisites. h. ciferrii was completely deficient in thiamine, biotin, inositol, riboflavin, niacin, and partially deficient in pantothenic acid. it required an external supply of 0.1-1.0 ppm thiamine, 0.01-0.1 ppm biotin, 10 ...19807242379
pathogenicity of some ordinarily harmless yeasts for cortisone-treated mice.the pathogenicity for normal and cortisone-treated mice of candida requinyii, pichia etchellsii, candida famata (torulopsis candida) trichosporon cutaneum and sterigmatomyces aphidis, isolated from foodstuffs or clinical material, is reported. the test species proved nonpathogenic to normal mice inoculated intravenously with up to 10(7) cells. there was neither any mortality nor gross or microscopic lesions, and cultures were negative from the visceral organs and brain when these animals were ne ...19807455862
pathogenicity of some ordinarily harmless yeasts for cortisone-treated mice.the pathogenicity for normal and cortisone-treated mice of candida requinyii, pichia etchellsii, candida famata (torulopsis candida) trichosporon cutaneum and sterigmatomyces aphidis, isolated from foodstuffs or clinical material, is reported. the test species proved nonpathogenic to normal mice inoculated intravenously with up to 10(7) cells. there was neither any mortality nor gross or microscopic lesions, and cultures were negative from the visceral organs and brain when these animals were ne ...19807455862
fungi in activated sludge biocenosis.it has been found that yeasts and yeast-like microorganisms are stable constituents of activated sludge biocenosis treating municipal wastewaters with a mixture of different industrial wastewaters at b upsilon values in the range of 1.3-1.7 kg cod m-3day-1 and b chi = 0.22-0.42 kg cod kg-1day-1. the number of these microorganisms reached about 10(4) cells mg vss-1 and as typical species saccharomyces cerevisiae and candida famata can be considered. considerably less numerous (4-8 cells mg vss-1) ...19937516619
fungi in activated sludge biocenosis.it has been found that yeasts and yeast-like microorganisms are stable constituents of activated sludge biocenosis treating municipal wastewaters with a mixture of different industrial wastewaters at b upsilon values in the range of 1.3-1.7 kg cod m-3day-1 and b chi = 0.22-0.42 kg cod kg-1day-1. the number of these microorganisms reached about 10(4) cells mg vss-1 and as typical species saccharomyces cerevisiae and candida famata can be considered. considerably less numerous (4-8 cells mg vss-1) ...19937516619
nadh dehydrogenase subunit genes in the mitochondrial dna of yeasts.the genes encoding the nadh dehydrogenase subunits of respiratory complex i have not been identified so far in the mitochondrial dna (mtdna) of yeasts. in the linear mtdna of candida parapsilosis, we found six new open reading frames whose sequences were unambiguously homologous to those of the genes known to code for nadh dehydrogenase subunit proteins of different organisms, i.e., nd1, nd2, nd3, nd4l, nd5, and nd6. the gene for nd4 also appears to be present, as judged from hybridization exper ...19947521869
hybrids obtained by protoplast fusion with a salt-tolerant yeast.an industrial strain of saccharomyces cerevisiae was fused with an osmotolerant yeast, debaryomyces hansenii, to obtain hybrids having increased tolerance to elevated salt concentrations. the hybrids were intermediate to parent species in production of ethanol and polyols.19957662292
response of bacteria and fungi to high-pressure stress as investigated by two-dimensional polyacrylamide gel electrophoresis.in an attempt to generalize previous observations (jaenicke et al., appl. environ. microbiol. 1988, 54, 2375-2380) and to find a convenient model system for studies of the pressure response, we tested the suitability of escherichia coli and thermotoga maritima (bacteria), and of five different eukaryotic species including the filamentous fungi asteromyces cruciatus and dendryphiella salina, and the marine yeasts debaryomyces hansenii, rhodosporidium sphaerocarpum, and rhodotorula rubra. using tw ...19947720693
effect of roasting and fermentation on viscosity of cereal-legume based food formulas.with the view of preparing semi-liquid weaning foods of high energy content, the influence of roasting (stationary hot air treatment) and fermentation (with natural and pure culture inocula) on the viscosity of maize-sorghum-soya porridges was investigated. roasting resulted in porridges of significantly higher viscosity (cooked porridge cooled to 40 degrees c). porridges made from the individual ingredients (maize, sorghum, soya) did not show this behaviour. natural fermentation of mixed ingred ...19947855078
effect of roasting and fermentation on viscosity of cereal-legume based food formulas.with the view of preparing semi-liquid weaning foods of high energy content, the influence of roasting (stationary hot air treatment) and fermentation (with natural and pure culture inocula) on the viscosity of maize-sorghum-soya porridges was investigated. roasting resulted in porridges of significantly higher viscosity (cooked porridge cooled to 40 degrees c). porridges made from the individual ingredients (maize, sorghum, soya) did not show this behaviour. natural fermentation of mixed ingred ...19947855078
the effect of hydroxycinnamic acids and potassium sorbate on the growth of 11 strains of spoilage yeasts.hydroxycinnamic acids and their derivatives occur widely in plants, fruits and wine. the effect of the common hydroxycinnamic acids (caffeic, coumaric and ferulic acids), at concentrations of 100 and 500 mg l-1, on growth of 11 strains of spoilage yeasts was measured spectrophotometrically and compared with that of potassium sorbate. ferulic acid was the most generally inhibitory hydroxycinnamic acid. at 500 mg l-1 it appreciably inhibited pichia anomala, debaryomyces hansenii and saccharomyces ...19957883649
[causal agents of onychomycosis].the results obtained with 307 specimens from putatively immunocompetent patients between may 1991 and may 1992 were reviewed, to determine the frequency of isolation of fungal species causing onychomycoses. sixty eight percent of the specimen were positive for microscopic examination and/or cultures. onychomycoses occurred with double frequency in women than in men (table 1), and 77% of cases were diagnosed in patients aged between 30 and 70 years (figure 1). out of 182 patients with positive cu ...19947938504
evaluation of two commercialized systems for the rapid identification of medically important yeasts.a total of 77 recent clinical isolates of candida albicans and other medically important yeasts were identified by two different commercial tests, rapidec albicans (api-biomérieux) and fongiscreen 4h (sanofi diagnostics pasteur), and conventional mycological methods. the strains were from 13 different species of yeasts and consisted of strains of 36 c. albicans, three of candida famata, nine of candida (torulopsis) glabrata, five of candida guilliermondii, two of candida kefyr, three of candida ...19938015561
evaluation of two commercialized systems for the rapid identification of medically important yeasts.a total of 77 recent clinical isolates of candida albicans and other medically important yeasts were identified by two different commercial tests, rapidec albicans (api-biomérieux) and fongiscreen 4h (sanofi diagnostics pasteur), and conventional mycological methods. the strains were from 13 different species of yeasts and consisted of strains of 36 c. albicans, three of candida famata, nine of candida (torulopsis) glabrata, five of candida guilliermondii, two of candida kefyr, three of candida ...19938015561
fatal candida famata peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis who was treated with fluconazole. 19948038334
fatal candida famata peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis who was treated with fluconazole. 19948038334
linear dna plasmids from pichia etchellsii, debaryomyces hansenii and wingea robertsiae.linear dna plasmids were found in the following yeasts: four strains of kluyveromyces lactis, one of debaryomyces hansenii, one of wingea robertsiae and four of pichia etchellsii. in each case, the plasmids were present as a pair of dna molecules of different sizes. the plasmids of k. lactis strains were associated with a killer activity and their structure was similar to the known killer plasmids pgkl1 and 2. the plasmids from the other three species were different from pgkl plasmids and showed ...19948081497
[autochthonous yeasts isolated in tenerife wines and their influence on ethyl acetate and higher alcohol concentrations analyzed by gas chromatography].a taxonomic study of yeasts present on tenerife wines, (tacoronte-acentejo specific denomination) has been carried out. nine species of the genera: saccharomyces, torulaspora, brettanomyces, kluyveromyces, debaryomyces, saccharomycodes, hansenula, pichia and candida have been isolated. parallely we analysed volatile compounds of the wines such as ethyl acetate, methanol, isobutanol and amylic alcohols by gas chromatography. appreciable quantities of ethyl acetate were detected due to the low fer ...19938172687
[in vitro susceptibility to fluconazole of fungal strains freshly isolated from child patients with deep-seated mycoses].the susceptibility to fluconazole (flcz) were evaluated for 40 strains of 8 yeast species and 4 strains of 3 aspergillus species, all of which were isolated from clinical specimens during clinical trials of flcz in child patients performed from january 1991 to january 1993 in this country. the in vitro activity of flcz against yeast isolates and aspergillus isolates were measured using the microdilution method with semisolid saamf (synthetic amino acid medium fungal) agar and the macrodilution m ...19938230734
[in vitro susceptibility to fluconazole of fungal strains freshly isolated from child patients with deep-seated mycoses].the susceptibility to fluconazole (flcz) were evaluated for 40 strains of 8 yeast species and 4 strains of 3 aspergillus species, all of which were isolated from clinical specimens during clinical trials of flcz in child patients performed from january 1991 to january 1993 in this country. the in vitro activity of flcz against yeast isolates and aspergillus isolates were measured using the microdilution method with semisolid saamf (synthetic amino acid medium fungal) agar and the macrodilution m ...19938230734
ability to assimilate nitrogenous oxides is limited to a few species of yeasts.several species of yeasts, saccharomyces cerevisiae, debaryomyces hansenii, candida utilis, c. albicans, c. pelliculosa and c. tropicalis, were tested for their ability to utilise different inorganic nitrogen compounds as sole sources of nitrogen, and the results are presented as a function of their growth. all of them grew well on reduced nitrogenous compounds such as ammonium sulphate (ammonia) and l-asparagine, while only c. utilis and c. pelliculosa grew, at a slower rate, on oxidised nitrog ...19938366830
osmophilic linear plasmids from the salt-tolerant yeast debaryomyces hansenii.three novel linear plasmids, pdhl1 (8.4 kb), pdhl2 (9.2 kb) and pdhl3 (15.0 kb), were discovered in the halophilic (salt-tolerant) yeast debaryomyces hansenii. exonuclease treatment indicated that all three plasmids were blocked at their 5' ends, presumably, by analogy with most other eukaryotic linear plasmids which involved protein attachment. the debaryomyces plasmids were entirely cured simply by growing cells in normal culture medium, but were stably maintained in culture medium containing ...19938391396
survey of the physiological properties of the most frequent yeasts associated with commercial chilled foods.a comparative analysis of the initial and final population of yeasts, lactic acid bacteria and psychrotrophic bacteria, in a number of chilled foods, varying in ingredients, physico-chemical characters and origin, gave evidence that yeasts could play a significant role in the spoilage. the yeast populations appeared to be unexpectedly uniform and comprised principally strains of yarrowia lipolytica, debaryomyces hansenii and pichia membranaefaciens. a survey of 62 isolates, comprising physiologi ...19938466806
ascomycetous yeast communities of marine invertebrates in a southeast brazilian mangrove ecosystem.the ascomycetous yeast communities associated with 3 bivalve mollusk, and 4 crab species were studied in the mangrove at coroa grande on sepetiba bay in rio de janeiro, brazil. these were made up mostly of diverse but sparse and apparently allochtonous yeast populations. the striking exception was a prevalent population of the species kluyveromyces aestuarii, which predominated the yeast communities of 2 detritus feeding crabs, sesarma rectum and uca spp., and the shipworm neoteredo reynei. howe ...19958546456
ascomycetous yeast communities of marine invertebrates in a southeast brazilian mangrove ecosystem.the ascomycetous yeast communities associated with 3 bivalve mollusk, and 4 crab species were studied in the mangrove at coroa grande on sepetiba bay in rio de janeiro, brazil. these were made up mostly of diverse but sparse and apparently allochtonous yeast populations. the striking exception was a prevalent population of the species kluyveromyces aestuarii, which predominated the yeast communities of 2 detritus feeding crabs, sesarma rectum and uca spp., and the shipworm neoteredo reynei. howe ...19958546456
use of chromagar candida medium for isolation of yeasts from dental samples.a new differential medium, chromagar candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. the recovery of yeasts on the medium was not significantly different from the recovery on sabouraud dextrose agar. the identities of 450 green colonies on chromagar candida, presumptively identified as candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-n-acetylgalacto ...19958576366
use of chromagar candida medium for isolation of yeasts from dental samples.a new differential medium, chromagar candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. the recovery of yeasts on the medium was not significantly different from the recovery on sabouraud dextrose agar. the identities of 450 green colonies on chromagar candida, presumptively identified as candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-n-acetylgalacto ...19958576366
three-dimensional structure of tubular networks, presumably golgi in nature, in various yeast strains: a comparative study.in the yeast saccharomyces cerevisiae, the golgi apparatus consists of discrete units distributed throughout the cytoplasm. when such units are examined in three dimensions, in relatively thick sections prepared for the electron microscope, they usually appear as small tubular networks with a stained material accumulating in dilations located at the junctions of membranous tubules. to see whether such tubular networks are observed in other yeast species, the three-dimensional structure of organe ...19958579247
[ferrireductase from pichia guilliermondii: properties and regulation of activity and synthesis].properties of pichia guilliermondii ferrireductase of the crude extracts and ammonium sulfate preparations were studied. nadh and cu (ii) ions are necessary for ferrireductase activity. mg (ii) also stimulates this reaction while oxygen acts as a slight inhibitor. ferrireductase reduces fe (iii) in complex with citrate, iron-binding peptide from the culture medium of p. guilliermondii, rhodotorulic acid, coprogen, desferrioxamine b and edta. mutants rib80, rib81 and hit of pichia guilliermondii ...19958588251
[ferrireductase from pichia guilliermondii: properties and regulation of activity and synthesis].properties of pichia guilliermondii ferrireductase of the crude extracts and ammonium sulfate preparations were studied. nadh and cu (ii) ions are necessary for ferrireductase activity. mg (ii) also stimulates this reaction while oxygen acts as a slight inhibitor. ferrireductase reduces fe (iii) in complex with citrate, iron-binding peptide from the culture medium of p. guilliermondii, rhodotorulic acid, coprogen, desferrioxamine b and edta. mutants rib80, rib81 and hit of pichia guilliermondii ...19958588251
the effect of substrate on the radiation resistance of yeasts isolated from sausage meat.the radiation resistance of a selection of yeasts isolated from sausages was assessed in phosphate-buffered saline and in sausage meat. the yeasts candida zeylanoides, debaryomyces hansenii and trichosporon cutaneum exhibited sigmoidal survival curves in both substrates whilst the more sensitive sporobolomyces roseus exhibited an exponential survival curve in buffer but a sigmoidal curve in meat. irradiating c. zeylanoides, d. hansenii and t. cutaneum in sausage meat changed the shape of their s ...19968588892
the occurrence and growth of yeasts in camembert and blue-veined cheeses.yeast populations greater than 10(6) cfu/g were found in approximately 54% and 36%, respectively in surface samples of retail camembert (85 samples) and blue-veined (45 samples) cheeses. the most predominant species isolated were debaryomyces hansenii, candida catenulata, c. lipolytica, c. kefyr, c. intermedia, saccharomyces cerevisiae, cryptococcus albidus and kluyveromyces marxianus. the salt concentration of the surface samples of the cheeses varied between 2.5-5.5% (w/w) (camembert) and 7.5- ...19968652347
ability of rapid yeast plus system to identify 304 clinically significant yeasts within 5 hours.the rapid yeast plus system (innovative diagnostic systems, norcross, ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. the ability of the rapid yeast plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. the rapid yeast plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. a further 12 strains (3.9%) were classif ...19968727877
ability of rapid yeast plus system to identify 304 clinically significant yeasts within 5 hours.the rapid yeast plus system (innovative diagnostic systems, norcross, ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. the ability of the rapid yeast plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. the rapid yeast plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. a further 12 strains (3.9%) were classif ...19968727877
flocculation of the yeast candida famata (debaryomyces hansenii): an essential role for peptone.aggregation of candida famata (debaryomyces hansenii) is consistent with being a form of lectin-mediated yeast flocculation. flocculation of c. famata is unusual in that it requires the presence of peptone, either in the growth medium or added later to harvested cells in buffer. flocculation after peptone addition was rapid, being largely complete within 10 min. heat-killed cells also flocculated, arguing for direct participation of peptone in the flocculation binding mechanism. flocculent c. fa ...19968740415
flocculation of the yeast candida famata (debaryomyces hansenii): an essential role for peptone.aggregation of candida famata (debaryomyces hansenii) is consistent with being a form of lectin-mediated yeast flocculation. flocculation of c. famata is unusual in that it requires the presence of peptone, either in the growth medium or added later to harvested cells in buffer. flocculation after peptone addition was rapid, being largely complete within 10 min. heat-killed cells also flocculated, arguing for direct participation of peptone in the flocculation binding mechanism. flocculent c. fa ...19968740415
yeast population during ripening of dry-cured iberian ham.the relationship between the superficial yeast population and the ripening conditions of iberian dry cured hams has been studied for three different locations. tentative identifications were carried out for 836 isolates. candida zeylanoides was the dominating yeast in early stages, whereas more than 99% of isolates from the surface of matured hams were identified as debaryomyces hansenii. a great diversity of strains of c. zeylanoides and d. hansenii was found. the characteristic pattern of isol ...19968796428
inhibitory effects of vanillin on some food spoilage yeasts in laboratory media and fruit purées.the effect of vanillin and essential oil of mint on the growth of different strains of food spoilage yeasts in laboratory media and fruit purées was studied. growth of saccharomyces cerevisiae, zygosaccharomyces rouxii, debaryomyces hansenii and z. bailii was inhibited in culture media and apple purée containing 2000 ppm of vanillin for 40 days storage at 27 degrees c and a(w) 0.99 or 0.95. but 3000 ppm of the spice were not effective to inhibit z. bailii growth in banana purée. growth of yeasts ...19968796437
evaluation of a commercial medium for identification of candida species.chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ...19968801088
evaluation of a commercial medium for identification of candida species.chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ...19968801088
utilization of acetonitrile and other aliphatic nitriles by a candida famata strain.a variant of a yeast strain identified as candida famata isolated from gold mine effluent was able to grow on acetonitrile, acrylonitrile, butyronitrile, isobutyronitrile, methacrylnitrile, propionitrile, succinonitrile, valeronitrile, acetamide, isobutyamide, and succinamide as sole nitrogen source, after acclimatization. the yeast grew on acetonitrile and acetamide at concentrations up to 4%. the utilisation of acetonitrile and acetamide by the c. famata strain probably involves hydrolysis in ...19968870254
utilization of acetonitrile and other aliphatic nitriles by a candida famata strain.a variant of a yeast strain identified as candida famata isolated from gold mine effluent was able to grow on acetonitrile, acrylonitrile, butyronitrile, isobutyronitrile, methacrylnitrile, propionitrile, succinonitrile, valeronitrile, acetamide, isobutyamide, and succinamide as sole nitrogen source, after acclimatization. the yeast grew on acetonitrile and acetamide at concentrations up to 4%. the utilisation of acetonitrile and acetamide by the c. famata strain probably involves hydrolysis in ...19968870254
random amplified polymorphic dna analysis of culture collection strains of candida species.random amplified polymorphic dna (rapd) profiles, obtained when screening culture collection strains of candida spp., showed several species to be highly heterogeneous. the rapd-defined groups were as follows: c. catenulata, two groups among five strains; debaryomyces hansenii (anamorph c. famata), three groups among five strains; c. sake, three groups among three strains; c. intermedia, two groups among two strains; c. rugosa, two groups among three strains and c. parapsilosis, three groups amo ...19968873891
growth of yeasts in milk and associated changes to milk composition.the growth of several yeast species in milk containing added sodium chloride (0-15%, w/v) at 25 degrees c and 10 degrees c was examined in conjunction with yeast metabolism of milk constituents. depending on conditions, all yeasts grew to maximum populations of 10(7)-10(8) cfu/ml. kluyveromyces marxianus gave strong utilisation of lactose and weak metabolism of citrate, protein and fat with the production of ethanol, glycerol, lactic acid and propionic acid. as measured by the production of free ...19968880309
the combined effects of environmental conditions on lipolysis of pork fat by lipases of the meat starter culture organisms staphylococcus xylosus and debaryomyces hansenii.the effects of environmental conditions on lipolysis by cell-free extracts from the meat starter culture organisms staphylococcus xylosus and debaryomyces hansenii were studied using pork fat emulsions as model systems. for the individual effects of temperature and ph it was found that the optimal conditions for the lipolysis by s. xylosus lipase were 37 degrees c and ph 7.0, and 37 degrees c and ph 6.5 for the lipolysis by d. hansenii lipase. for the combined effects of conditions relevant to m ...19968880328
phylogenetic relationships among members of the ascomycetous yeast genera brettanomyces, debaryomyces, dekkera, and kluyveromyces deduced by small-subunit rrna gene sequences.a molecular systematic investigation of members of the ascomycetous yeast genera brettanomyces, debaryomyces, dekkera, and kluyveromyces was performed by using 18s rrna gene sequence analysis. our comparative sequence analysis revealed that brettanomyces anomalus and brettanomyces bruxellensis were closely related to one another and also to their teleomorphs, dekkera anomala and dekkera bruxellensis, respectively. together with dekkera custersiana and dekkera naardenensis, these four species for ...19968934908
structure and antigenicity of the mannans of candida famata and candida saitoana: comparative study with the mannan of candida guilliermondii.the chemical structure of the mannans of antigenic factor 9-expressing yeast, candida famata and candida saitoana, was analyzed by acetolysis and nmr. the structural study of the oligosaccharides and mannans using one- and two-dimensional nmr indicated that the mannan of c. saitoana contains a third type of beta-1,2-linked mannose unit. on the other hand, the mannan of c. famata does not contain any beta-1,2-linked mannose units. the mannan of c. saitoana gave two groups of beta-1,2 linkage-cont ...19968951034
structure and antigenicity of the mannans of candida famata and candida saitoana: comparative study with the mannan of candida guilliermondii.the chemical structure of the mannans of antigenic factor 9-expressing yeast, candida famata and candida saitoana, was analyzed by acetolysis and nmr. the structural study of the oligosaccharides and mannans using one- and two-dimensional nmr indicated that the mannan of c. saitoana contains a third type of beta-1,2-linked mannose unit. on the other hand, the mannan of c. famata does not contain any beta-1,2-linked mannose units. the mannan of c. saitoana gave two groups of beta-1,2 linkage-cont ...19968951034
taxonomic position of clinical isolates of candida famata.we examined five clinical isolates formerly classified as candida famata to clarify their taxonomic position by dna-dna reassociation studies and several phenotypic features. four of the five isolates, m 5101, m 5102, m 5111 and m 5112, produced ascospores after being grown for 1 week on ym agar at 20 degrees c. by the api 20c system, three of the five isolates were identified as c. famata, and the other two strains as c. guilliermondii/c. famata. the antigenic patterns of all the isolates exami ...19968971631
taxonomic position of clinical isolates of candida famata.we examined five clinical isolates formerly classified as candida famata to clarify their taxonomic position by dna-dna reassociation studies and several phenotypic features. four of the five isolates, m 5101, m 5102, m 5111 and m 5112, produced ascospores after being grown for 1 week on ym agar at 20 degrees c. by the api 20c system, three of the five isolates were identified as c. famata, and the other two strains as c. guilliermondii/c. famata. the antigenic patterns of all the isolates exami ...19968971631
[the yeast flora of blood-sucking mosquitoes].yeast flora composition has been studied in natural populations of blood-sucking mosquitoes of genera aedes. anopheles and culex dwelling in the territory of ukraine. the authors have distinguished 18 yeast species belonging to 7 genera. species candida famata, c. (yarrowia) lipolytica as well as the yeast of genera rhodotorula and cryptococcus dominate on the larvae and imago of mosquitoes. the distinguished strains are the components of normal microflora of blood-sucking mosquitoes and take no ...19968983519
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