biosynthesis of flavins and its regulation in the yeast pichia guilliermondii. | the nature of riboflavin precursors was studied in the yeast pichia guilliermondii. by means of mutants with blocked gmp-synthetase the purine precursors of riboflavin were shown to belong to guanylic compounds. accumulation of 2,4,5-triamino-6-oxypyrimidine, 2,5-diamino-6-oxy-4-ribitylaminopyrimidine, 2,6-dioxy-5-amino-4-ribitylaminopyrimidine (doarap) and 6,7-dimethyl-8-ribityllumasine occurs in the riboflavin-deficient mutants divided into five biochemical groups. this fact evidences for iden ... | 1975 | 834 |
specific p-nitrophenyl phosphatase of yeast pichia guilliermondii. | the cells of yeast p. guilliermondii contain specific p-nitrophenyl phosphatase (pnppase), the level of which depends on the cells supply with inorganic phosphorus. partially purified enzyme is activated by ions mg2+, co2+ and somewhat weaker -- by ions fe2+. with the presence of mg2+ the enzyme activity is inhibited by ions cd2+, zn2+, f-, be2+, cu2+, mn2+, ca2+, moo42-, fe3+, fe2+, inorganic phosphate as well as by edta. a mixture ions be2+ and f- causes a complete inhibition of the activity. ... | 1975 | 1885 |
regulation of 6-hydroxy-2,4,5-triaminopyrimidine synthesis by riboflavin and iron in riboflavin-deficient mutants of pichia guilliermondii yeast. | the effect of riboflavin and iron on 6-hydroxy-2,4,5-triaminopyrimidine synthesis rate was investigated in the cultures of the yeast pichia guilliermondii (rib2 mutants) with the blocked second reaction to flavinogenesis. it was shown that riboflavin inhibited the 6-hydroxy-2,4,5-triaminopyrimidine synthesis rate in iron-rich and iron-deficient cells of mutants with low riboflavin requirements. cycloheximide did not prevent the stimulation of 6-hydroxy-2,4,5-triaminopyrimidine synthesis caused b ... | 1976 | 6053 |
purification and properties of the riboflavin kinase of the yeast pichia guilliermondii. | riboflavin kinase (e.c.2.7.1.26) was isolated from the cells of the yeast pichia guilliermondii. the enzyme was 680-fold purified uzing ammonium sulphate fractionation, chromatography on deae-sephadex a-50 and cm-sephadex c-50 and gel-filtration through sephadex g-75. purified enzyme preparation was free from phosphatases and fad-synthetase. the ph optimum was 8,7, the temperature optimum-45 degrees c. the enzyme was activated by zn2+, mg2+ and co2+ ions. km for riboflavin was 1,0x10(-5) m, for ... | 1976 | 6079 |
study of acid phosphatase ii from pichia guilliermondii yeasts. | acid phosphatase ii (ap ii) was isolated from the cell-free extract of pichia guilliermondii wickerham atcc 9058 and partially purified. the enzyme is a non-specific phosphomonoesterase. it hydrolyzes p-nitrohenyl-phophate (npp), beta-glycerophosphate, glucose-6-phosphate, guanosine-5'-monophosphate, adenosine-5'-monophosphate, cytidine-5'-monophosphate, uridine-5'-monophosphate, alpha-naphtylphosphate, fmn. the order of the substrates corresponds to the degree of their hydrolysis decrease. the ... | 1976 | 8863 |
anabolic and catabolic enzymes of urea metabolism in a carbohydrate-utilizing strain of candida guilliermondii. | the yeast "h" of the genus candida guilliermondii can grow on hydrocarbons as the only source for carbon. urea can serve as a nitrogen source for this yeast which lacks detectable urease activity. during urea metabolism ammonia has never been accumulated in the culture medium. however, transferring the yeast from complete urea-medium into an urea containing phophate-buffer, the degradation of urea continues and ammonia is accumulated as well as co2 evolved. in cell-free extracts of the yeast ure ... | 1977 | 24924 |
dicarboxylic acid formation by candida guilliermondii h strain h 17 during the degradation of n-tridecane in batch culture. | | 1978 | 25511 |
cultivation of feed yeasts on media with carbohydrates. | the different culture of yeasts oxidizing hydrocarbons and the accompanying microflora are, under certain physico-chemical conditions, in metabiotic relationships which provide stability of the system. these relationships were studied with yeast cultures belonging to the candida genus and oxidizing hydrocarbons. the relationships between the hydrocarbon oxidizing yeast candida guilliermondii and other species of the genus candida were determined by a combination of active and passive antogonism, ... | 1978 | 26855 |
cytochrome p-450 and alkane hydroxylase activity in candida guilliermondii. | in the investigated candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nmol cytochrome p-450 per g cells (wet weight) could be detected. cytochrome p-450 and alkane hydroxylase activity was found in the 100 000 xg pellet. cofactor studies and inhibition experiments revealed the existence of a nadph-dependent cytochrome p-450 alkane hydroxylase system. | 1978 | 30246 |
origin of the ribityl side-chain of riboflavin from the ribose moiety of guanosine triphosphate in pichia guilliermondii yeast. | in wild-type cells and some riboflavin-deficient mutants of p. guilliermondii gtp is transformed to the ribitylated intermediates 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine and 5-amino-2,6-dihydroxy-4-ribitylaminopyrimidine of the riboflavin biosynthetic path. we were able to show that these compounds were formed in vitro as well as in permeabilized cells by reactions including a reductive conversion of the product of gtp cyclohydrolase ii action upon gtp. in order to analyse the pyrimidine ... | 1978 | 30490 |
[effect of glucose and its derivatives on systems of riboflavin uptake and excretion in the yeast pichia guilliermondii]. | riboflavin uptake by washed cells of riboflavin deficient mutant ms1-3 of pichia guilliermondii yeast was strongly depressed by d-glucose, l-sorbose, alpha-methyl-d-glucoside, sucrose, trehalose, maltose and salicin but not by d-mannose, d-galactose, d-fructose or ribitol. glucose decreased also the initial uptake rate of riboflavin analogue, 8-piperidyl-10-(1'-d-galactityl) isoalloxazine; the inhibition having a competitive character (ki==5,7 mm). apparently riboflavin permease is able to accep ... | 1978 | 32927 |
[studies on lytic enzyme activities of bacteria and streptomycetes against candida guilliermondii after partial purification with ammonium sulfate from shaking- and fermentation cultures (author's transl)]. | two bacteria and two streptomycetes strains, capable to degrade cell walls of candida guilliermondii, were isolated from soils. growth and enzyme production of these strains were investigated on synthetic and complex media in shaking culture and under fermentation conditions. the isolated microorganisms possessed lytic enzyme activities against living and autoclaved yeast cells. the lytic enzyme complex of the culture medium of microorganisms was partially purified by ammonium sulfate precipitat ... | 1979 | 44474 |
biosynthesis of beta-phenethyl alcohol in candida guilliermondii. | | 1976 | 57780 |
defective neutrophil function in chronic granulocytic leukaemia. | the ability of neutrophils to phagocytose, kill and digest candida guilliermondii was investigated in twelve patients with chronic granulocytic leukaemia (cgl). following ingestion of organisms there was considerable reduction in the ability of cgl cells to kill candida and this was not explained by a mild impairment of phagocytosis. histochemical staining showed that granules containing lysosomal enzymes disappear form the cytoplasm of normal neutrophils during killing and digestion of the fung ... | 1976 | 60123 |
[on the similarity of antigen between fungi and salmonella. 4. communication (author's transl)]. | the immune sera for candida membranefaciens (c. majoricensis), candida guilliermondii var. carpophila, metschnikowia bicuspidata, syringospora stellatoidea and torulopsis candida agglutinated salmonella cholerae-suis (6,7). the immune serum for s. cholerae-suis agglutinated candida membranefaciens (c. majoricensis), candida guilliermondii var. carpophila, metschnikowia biscuspidata, syringospora stelladoidea and torulopsis candida. absorption and agglutination cross test demonstrated common anti ... | 1976 | 65072 |
[coordinate regulation of riboflavin permease and alpha-glucosidase synthesis in the yeast pichia guilliermondii]. | riboflavin permease of the yeast pichia guilliermondii appear to be inducible transport system. its synthesis is induced by sucrose, maltose, alpha-methyl-d-glocoside, melizitose and raffinose, but not by d-glucose, trehalose or cellobiose. the synthesis of riboflavin permease in the presence of sucrose of maltose is depressed by cycloheximide, actinomycin d and 8-hydroxyquinoline. these results suggest that the synthesis of riboflavin permease is regulated on the transcription level. the induce ... | 1979 | 116690 |
the alkane-hydroxylating enzyme system of the yeast candida guilliermondii. | the growth of the investigated candida guilliermondii strain on n-alkanes induces an alkane-hydroxylating enzyme system, which consists of a cytochrome p-450 and a nadph-dependent reductase. the cytochrome p-450 was purified to 4 nmoles per mg protein. long-chain alkanes, preferably hexadecane to octadecane, are hydroxylated to the corresponding primary alcohol by this enzyme system. the substrate induces a type i spectrum, other compounds checked type ii spectra. | 1979 | 117657 |
microbicidal function of the neutrophils in hairy-cell leukaemia. | the ability of neutrophils from patients with hairy-cell leukaemia to kill candida guilliermondii and staphylococcus aureus in vitro was investigated. of 10 patients, 8 showed defective candida killing, 5 defective bacteria killing, in comparison with normal controls. study of those patients who underwent splenectomy suggests that neutrophil function can be improved by splenectomy. the possible causes of defective function in this disease are discussed. | 1979 | 119411 |
[paraffin oxidizing system of the yeast candida guilliermondii]. | it has been demonstrated that the enzyme system of candida guilliermondii responsible for hydrocarbon oxidation involves nadph-cytochrome c-reductase (ec 1623) and cytochrome p-450. the system is located in the microsomal fraction. cytochrome p-450 synthesis is induced by hexadecane occurring in the medium. the cellular content of cytochrome p-450 varies in the course of the culture growth. there is a correlation between the cellular content of cytochrome p-450 and the synthesis of primary produ ... | 1979 | 119961 |
[determination of the relative volume of the incorporations of a lipid nature by candida guilleirmondii yeast cultured on hydrocarbons]. | the paper describes a method for measuring a relative volume of lipid incorporations into yeast candida guilliermondii cultivated on hydrocarbons. a relative volume of the incorporations (mean ratio of the volume of lipid incorporations to the cell volume expressed as percentage) as compared with the hydrocarbon content in the cells expressed as percentage of the yeast dry weight is given for each yeast sample. a direct correlation between the hydrocarbon content and relative volume of lipid inc ... | 1975 | 171643 |
[activities of some yeast flavogenic enzymes in situ]. | effects of digitonin, dimethylsulfoxide and protamine sulfate on yeast pichia guilliermondii were studied in order to produce cells with increased permeability and possessing the gtp-cyclohydrolase, riboflavinsynthetase and riboflavinkinase activities. the digitonin-treated cells exhibited a higher cyclohydrolase activity than the cell-free extracts; the activities of riboflavinsynthetase and riboflavinkinase in the cells and cell-free extracts were found to be similar. treatment of cells with d ... | 1977 | 199288 |
impaired neutrophil function and myeloperoxidase deficiency in myeloid metaplasia. | the ability of neutrophils to phagocytose and kill candida guilliermondii was investigated in 12 patients with myeloid metaplasia (mm). following ingestion there was a considerable impairment in the ability of mm neutrophils to kill and digest candida which was not explained by the very mild impairment in phagocytosis. quantitative myeloperoxidase measurement revealed an overall deficiency of this enzyme in mm neutrophils and a highly significant correlation between low myeloperoxidase levels an ... | 1977 | 203312 |
[effect of oxygen on pyrophosphate and atp accumulation in candida guilliermondii yeasts cultivated on petroleum paraffins]. | | 1978 | 207500 |
[inhibition of alkaline phosphatase i of pichia guilliermondii yeast in vitro and in vivo]. | the rate of p-nitrophenyl phosphate and flavin mononucleotide (fmn) hydrolysis by the partially purified preparation of alkaline phosphatase i of pichia guilliermondii flavinogenic yeast was studied as affected by different substrates and inorganic ions. their km was established to be 2.0 x 10(-4) m and 2.5 x 10(-4) m, respectively. dephosphorylation of p-nitrophenylphosphate and fmn was inhibited competitively by beta-glycerophosphate (ki = 3.1 x 10(-3) m, respectively). the presence of inorgan ... | 1978 | 208203 |
[substrate and inhibitory specificity of riboflavin kinase from pichia guilliermondii yeast]. | the interaction of purified riboflavin kinase (ec 2.7.1.26) from pichia guilliermondii with 44 structural vitamin b2 analogues is studied. the presence of d-ribityl lateral chain in an analogue structure is found to be necessary for the substrate activity. the substitution of ch3 groups in the 7 and 8 positions of isoalloxazine ring in the riboflavin molecule for cf3, cl, h, nh2 and n(ch3)2 resulted in the decrease of the analogue affinity to riboflavin kinase as compared with the natural substr ... | 1978 | 217452 |
[activity of certain dehydrogenases in accumulation and continuous-flow culture of paraffin-oxidizing yeast of the genus candida]. | lability of nadp- and nad-dependent glutamate dehydrogenases and malate dehydrogenases has been demonstrated during a change of the physiological activity of yeast candida guilliermondii in the exponential phase of growth. variations in the isoenzyme spectrum of the enzymes and total dehydrogenase activity of the yeast has been found during their transition from the accumulative to the continuous cultivation. changes in the physiological state of yeast during their growth are accompanied by a re ... | 1975 | 241990 |
defective hydrogen peroxide production in chronic granulocytic leukaemia neutrophils. | hydrogen peroxide (h2o2) production by polymorphonuclear neutrophils (pmn) during phagocytosis was fluorometrically vestimated over an incubation period of 80 min during which dead candida guilliermondii were ingested. ten patients with chronic granulocytic leukaemia (cgl) were tested and compared with 15 normal subjects. the production of h2o2 by cgl neutrophils was about 29% of normal. there was no correlation between h2o2 production and the impaired killing of candida guilliermondii by the ne ... | 1979 | 283836 |
experimental hematogenous endophthalmitis due to candida: species variation in ocular pathogenicity. | the ocular pathogenicity of species of candida other than albicans was studied in the rabbit model of hematogenous candida endophthalmitis by injection of 10(5)-10(8) colony-forming units of candida krusei, candida parapsilosis, candida guilliermondii, candida tropicalis, candida stellateoidea, and candida albicans (control). at one and three weeks after infection, rabbits were examined by indirect ophthalmoscopy and were sacrificed for culture of eyes and kidneys. with an inoculum of 10(8) orga ... | 1977 | 320277 |
[riboflavin transport in cells of riboflavin-dependent yeast mutants]. | riboflavin was transported at a high rate into yeast cells of pichia guilliermondii and schwanniomyces occidentalis mutants capable of growth in a medium containing low concentrations of riboflavin, and having multiple susceptibility to some antibiotics and antimetabolites. sucrose and sodium azide inhibited transport of riboflavin. other riboflavin dependent mutants of pichia guilliermondii, pichia ohmeri, torulopsis candida, and saccharomyces cerevisiae, also growing in media containing low co ... | 1977 | 329070 |
[comparative study of the protein makeup in diploid and haploid forms of saccharomyces and pichia]. | the rates of growth, biomass accumulation, and electrophoretic spectra of mobile cytoplasmic proteins were studied with nonisogenous haploid and diploid cultures of saccharomyces cerevisiae and pichia guilliermondii as well as with isogenous haploid-diploid pairs of saccharomyces cerevisiae and pichia pinus. on a mineral medium with glucose, differences in these parameters in various yeast strains were found to be due to the genotype of a strain rather than to ploidy: nonisogenous haploid and di ... | 1979 | 384183 |
identification of species of candida, cryptococcus, and torulopsis by gas-liquid chromatography. | gas-liquid chromatography was used to identify species of candida, cryptococcus, and torulopsis by fatty-acid analysis of the whole-cell hydrolysate. candida albicans characteristically revealed 2-oh c14:0 and c19:0 (chain length:number of double bonds); these were absent in other organisms. candida curvata was characterized by a ratio of c16:1 to c16:0 of greater than 1.0. candida guilliermondii contained c10:0, and candida tropicalis had no c12:0, these features were used for their identificat ... | 1979 | 396339 |
affinity chromatographic purification of beta-glucosidase of candida gulliermondii. | a beta-glucosidase was isolated from candida guilliermondii, a yeast capable of growth on cellobiose. the enzyme was partially purified by treatment with polyethyleneimine and ammonium sulfate precipitation. further purification was achieved by affinity chromatography using a sepharose 4b matrix to which oxidized salicin was coupled through adipic dihydrazide. the final product was a 12.5-fold purification of the crude extract with a recovery of 27% of the initial enzyme activity. polyacrylamide ... | 1978 | 417311 |
[change in the morphological structure of the population and in the flavin level in the cells of candida guilliermondii yeasts under periodic cultivation]. | the growth of candida guilliermondii on media with glucose and acetate is described by statistical functions for cell volume (size) distribution. special instrument has been used to measure the volume of cells and volume distributions. changes in the morphological structure of populations (both in the average size of cells and in the extent of the size heterogeneity) were detected throughout the whole period of the cultural growth including the exponential phase. these changes hardly differed du ... | 1979 | 440160 |
[biomass accumulation by polyploid forms of candida guilliermondii yeasts]. | under the action of a mitotic poison (acenaphthene), the haploid culture of candida guilliermondii assimilating n-alkanes yielded a polyploid form whose cells were four times larger, on the average, than in the haploid culture. when the pure culture was grown under stationary conditions in media with glucose, the economical coefficient, i. e. the ratio between the assimilated glucose and the accumulated biomass, was by 16, 47 and 157% higher in the polyploid culture than in the haploid one after ... | 1979 | 470627 |
[taxonomic significance of the "hydrocarbon assimilation" trait for pichia guilliermondii wickerham yeasts]. | | 1979 | 481265 |
[product of second-stage riboflavin biosynthesis in pichia guilliermondii]. | dialyzed extracts of the mutants of pichia guilliermondii rg80 (rib3, his-) and ra39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted gtp into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of nadph2. apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydro ... | 1979 | 481291 |
[effect of polyene antibiotics and their perhydrovderivatives on intact cells and protoplasts of yeast candida guilliermondii]. | perhydroderivatives of polyene antibiotics have a much lower activity against eukaryotic cells than the polyene antibiotics itself. bacterial cells are normally resistant against most polyene antibiotics and their perhydroderivatives. in earlier experiments with wall less l-form cells of escherichia coli we have shown that the bacterial cell wall may be responsible for the resistance of the intact bacterial cells against polyene antibiotics and their perhydroderivatives by masking internal targe ... | 1979 | 483868 |
[effect of the polyene antibiotic, roseofungin, on the liberation of the intracellular components from candida guilliermondii cells and from rat erythrocytes]. | liberation of inorganic ions (potassium and phosphorus), nucleotides and protein from the cells of candida guilliermondii and rat erythrocytes was studied. it was found that leakage of these components depended on the incubation time, antibiotic dose and amount of the cell material. the potassium ions proved to be most sensitive to the antibiotic: even at low concentrations of roseofungin (2-4 microgram/ml) the amount of the intracellular potassium changed. a decrease in the dry mass and volume ... | 1979 | 485111 |
[genetic classification of riboflavin-dependent mutants of pichia guilliermondii yeasts]. | 114 riboflavinless mutants were selected from the genetic line of pichia guilliermondii yeast. by means of accumulation test the mutants were divided into five biochemical groups. in genetic experiments seven complementation classes were found among 106 mutants. the strains of the i biochemical group, accumulating no specific products, corresponded to complementation class rib1; ii group, accumulating 2,4,5-triaminopyrimidine - to the class rib2; iii group, accumulating 2,6-dihydroxy-4-ribitylam ... | 1979 | 488712 |
comparison of lipid composition of candida guilliermondii grown on glucose, ethanol and methanol as the sole carbon source. | the carbon and energy source for aerobically grown cultures of candida guilliermondii profoundly influenced the neutral lipid content and the fatty acid composition of the individual lipid components. methanol (0.80%, w/v) grown cells cultivated at 30 c in presence of 0.025% ammonium sulfate contained 12% total lipids, 67% of which was neutral lipids. glucose (0.74%), w/v) or ethanol (0.53%, w/v) grown cells contained 21--22% total lipids, 80% of which was neutral lipids, under the same conditio ... | 1979 | 513982 |
[effect of ubiquinones and their analogs on the respiratory chain enzyme activity of candida guilliermondii yeasts]. | the effect of ubiquinones with different length of their chain (coq0, coq1, coq2, coq6, coq9) and their synthetic analogues (analogues of ubiquinone-1, hexahydroubiquinone-4, monophytylquinone, diphytylquinone, triphytylquinone) on the activity of ubiquinone dependent enzyme systems was studied in mitochondrial fractions from the yeast candida guilliermondii. all of the ubiquinone homologues studied activated these systems. the synthetic analogues of ubiquinone nonspecifically inhibited the acti ... | 1979 | 530141 |
[regulation of synthesis of gtp-cyclohydrolase participating in yeast falvinogenesis by iron]. | pichia guilliermondii, schwanniomyces occidentalis, torulopsis candida and several riboflavin-dependent mutants of torulopsis candida were grown in a medium with a low concentration of iron. in these conditions, the activity of gtp-cyclohydrolase which catalyzes the first step of flavinogenesis increases. the activity of the enzyme increases also when the cells of t. candida and p. guilliermondii with a high content of iron are incubated with alpha, alpha'-dipyridyl which induces overproduction ... | 1977 | 561294 |
[preliminary and postradiation effect of long-wave ultraviolet rays on cells of different species of candida irradiated with short-wave ultraviolet rays]. | certain characteristics of photoprotection induced by near-uv were studied in candida guilliermondii. candida tropicalis was also capable of photoprotection from the lethal effect of far-uv. the spectrum of action of photoprotection was recorded. the photoprotective effect was not found in candida utilis. the postradiation effect of near-uv was studied. the lethal action of far-uv was intensified in c. guilliermondii and c. tropicalis incapable of photoreactivation in contrast to c. utilis capab ... | 1979 | 572905 |
repair of 313-nm induced lesions and photoprotection in yeast candida guilliermondii. | | 1977 | 594172 |
[the activity of enzymes involved in synthesis and hydrolysis of flavin adenine dinucleotide is pichia guilliermondii studies at different levels of flavinogenesis]. | the activity of fad-pyrophosphorylase and fad-hydrolase (nucleotidepyrophosphatase) was studied in extracts of pichia guilliermondii atcc 9058 capable of riboflavin over-production. the specific activity of the enzymes was highest at the logarithmic growth phase (2.6 and 3.8 mcmoles of fad per 1 min per 1 mg of protein x10(-5), respectively), and did not increase upon the induction of riboflavin overproduction. a decrease in the content of hemin compounds and a low content of flavins in the cell ... | 1977 | 600094 |
[antigenic relationship among candida guilliermondii strains (author's transl)]. | in this short communication the results of cross aglutination and absorption tests among three sandard candida guilliermondii strains (c. guilliermondii cbs--566, c. guilliermondii var. carpophila cbs--5256, c. guilliermondii var. japonica cbs--6021) are given. it showed that these microorganisms have the same antigenic structure. | 1977 | 600117 |
[mutants of pichia guilliermondii yeasts with multiple sensitivity to antibiotics and antimetabolites. i. the selection and properties of the mutants]. | riboflavin deficient mutant pichia guilliermondii ms1 which requires approximately 1000-fold lower concentration of exogenous vitamin b2 for growth when compared with a non-adapted riboflavin deficient mutants of this species was isolated by means of of uv-irradiation. the growth of the mutant was strongly inhibited by actinomycin d and l-canavanine. the revertant ms8 and ms14 which synthesized riboflavin were selected from the strain ms1. these revertants posses a multiple sensitivity to actino ... | 1977 | 611044 |
[structure of mannan of candida guilliermondii h]. | the mannan of candida guilliermondii was acetolyzed and the seperation by gel chromatography gave five neutral fragments with the characteristic nuclear magnetic resonance spectra, mannose, mannobiose, mannotriose, mannotetraose, and mannopentaose. all oligosaccharide fragments were composed of mannose in alpha-linkage. methylation analysis combined with gas chromatography-mass spectrometry demonstrated (1 leads to 6)-linked d-mannopyranose main chains with (1 leads to 2)-linked d-mannopyranose ... | 1978 | 645109 |
[role of endogenous substances in creating a background of increased radioresistance. 10. the nature of the varying radioresistance of yeast cells (experiments on pichia guilliermondii of varying ploidy)]. | | 1978 | 652974 |
[demonstration of alpha-mannosidase and beta-glucosidase in a yeast cell-wall lytic system of streptomyces spec. 5s]. | streptomyces spec. 5s accumulates in the culture fluid lytic activities against living cells of candida guilliermondii h. from the culture fluid a freeze-dried dialyzed ammonium sulfate precipitate was prepared. except beta-glucosidase-activity of alpha-mannosidase which was characterized. separation of both enzymes by chromatography on polyacrylamide gel was possible. | 1978 | 664754 |
[electron microscopic study of the action of submicroscopic levorin structures on candida guillermondii]. | in the course of preparation of aqueous solutions of the polyene antibiotic levorin, the latter is recovered in the solid phase forming granular submicroscopic structures. if the cells of candida guilliermondii are treated with submicroscopic granular structures (smgs) of levorin, the structures are adsorbed on the surface of the yeast cell walls. some visible changes occur in the ultrastructure of the yeast cells incubated with smgs of levorin for 5 min: the inner layer of the cell wall becomes ... | 1978 | 713884 |
defective microbicidal function of neutrophils in haematological malignancies and lymphomas: correction by levamisole in vitro. | the ability of neutrophils to kill candida guilliermondii was investigated in patients with various haematological malignancies and lymphomas. twelve of 24 patients showed significant depression of candida killing in vitro in comparison with normal controls. the addition of levamisole to the test system, in a concentration of 1 x 10(-7)m, resulted in the correction of the candida killing defect in 7 of 8 patients. | 1978 | 719129 |
[regulation of the activity of gtp-cyclohydrolase, the enzyme of the first step of flavinogenesis in yeasts]. | the kinetic properties and regulation of activity of gtp-cyclohydrolase, the enzyme of the first step of flavinogenesis in the pichia guilliermondii yests, partially purified by gel-filtration were studied. it was found that the curve of the dependence of reaction rate on substrate concentration is non-hyperbolic. fad inhibited the enzyme activity, while riboflavin and fmn had no such effect. in addition to fad, 5'-amp, 3',5'-amp, adp, atp, nad and nadp inhibited the enzyme activity. under combi ... | 1978 | 737222 |
[respiratory system of pichia guilliermondii yeasts with different levels of flavinogenesis]. | the yeast pichia guilliermondii was grown on media with different content of iron and its respiration system was studied. when the yeast was cultivated on a complete medium, its respiratory chain operated at the maximum rate in the exponential growth phase, i. e. all the three points of phosphorylation were involved; cytochrome oxidase was the only terminal oxidase. when the growth was decelerated and at the stationary phase, the alternative autooxidable cyanide-resistant pathway inhibited with ... | 1978 | 745565 |
role of opportunistic fungi in ocular infections in nigeria. | the importance of opportunistic fungal pathogens in causing ocular infections is emphasized. a study was conducted over a period of 4 years (1974--1977) to investigate the role of opportunistic fungi in causing mycotic keratitis and to elucidate certain aspects of epidemiology of this disease in nigeria. fifty-nine cases of corneal ulcers of suspected mycotic etiology were investigated. fungal etiology was confirmed in 42 of these cases. the predominant etiological agent was fusarium solani in 1 ... | 1978 | 745623 |
[excretion of organic compounds bei microorganisms in presence of diphenylamine]. | diphenylamine (dpa) causes an increased leakage of organic compounds with resting cells of candida guilliermondii, bacillus subtilis, escherichia coli, and staphylococcus aureus already after a treatment of 1 h. the leakage depends both on the concentration of dpa and the duration of the treatment. moreover, dpa influences the composition of the leaked ninhydrine-positive compounds. after 20 h of treatment an increased release of mannan and ribose is observed in addition to the stronger leakage ... | 1976 | 823713 |
[repair of damage caused by 313 nm light and photoprotection in candida guilliermondii]. | the study of the effect of near-uv light (300-380 nm) on the yeast candida guilliermondii has shown that certain doses of 313 nm light cause inactivation which is reversible after the cells have been kept for some time in a non-nutrient medium. the findings suggest a different nature of the lesions resulting in killing the cells irradiated with light of 313 and 254 nm. the data obtained are interpreted in terms of a new, yet unknown, repair mechanism, other than the classical dark reparation, wh ... | 1977 | 859465 |
[alterations in the excess synthesis of riboflavin in pichia guilliermondii under the influence of branched-chain amino acids]. | the effect of the branched-chain amino acids: l-valine, l-isoleucine and l-leucine on riboflavin overproduction was studied in the pichia (candida) guilliermondii (cast.) lang. et g. yeast, l-val, l-ile and l-leu were found to inhibit riboflavin overproduction only under iron-deficient growth conditions. other amino acids used did not show this effect. in crude extracts of p. guilliermondii the specific activity of the alpha-acetolactate forming enzyme, ph 8.0, is inhibited by l-val. it is revea ... | 1977 | 867527 |
[influence of carbon source on lipid biosynthesis by candida gluilliermondii]. | the biosynthesis of lipids by candida guilliermondii was studied during the growth of the yeast on carbon substrates which resulted in different pathways of lipid biosynthesis. the highest content of lipids was found during the growth on octadecane. the quantitative ratio between fractions of neutral lipids and phospholipids depended on the carbon substrate whereas the quanlitative composition of the fractions remained the same. the fatty acid composition of lipid fractions was studied, and poss ... | 1977 | 882010 |
[active transport of riboflavin in the yeast pichia guilliermondii. detection and some properties of the cryptic riboflavin permease]. | in contrast to cells and protoplasts of the wild strain intact cells and protoplasts of riboflavin (rf)-deficient mutants of pichia guilliermondii yeast possessing multiple sensitivity to antibiotics and antimetabolites were found capable to accomplish active transport of rf. the accumulation of rf against concentration gradient was energized by endogenous energy sources and was strongly depressed by uncouplers of oxidative phosphorylation and by inhibitors of respiration. rf transport was also ... | 1977 | 922070 |
[selection of a genetic strain of pichia guilliermondii yeasts capable of forming a significant quantity of spores]. | | 1977 | 929679 |
[a flavinogenic mutant of the yeast pichia guilliermondii with impaired iron transport]. | a mutant of the yeast pichia guilliermondii was produced by means of uv; the mutant was capable of riboflavin overproduction in the presence of high concentrations of iron in the medium. the content of total and non-hemin iron and cytochrome c, and the activity of catalase, were lower in the cells of the mutant than in the parent cells, while the activity of riboflavin synthetase was higher. the content of iron in the cells increased when the mutant was cultivated on media with citric acid, side ... | 1976 | 933879 |
phytochrome system of the yeast candida guilliermondii and recovery from ultraviolet injury. | the effect of red (660 nm) and far-red (730 nm) light on the stability of the yeast candida guilliermondii to lethal u.v. radiation has been studied. reactivation and protection were exhibited for 30 min after treatment with red light and were abolished by far-red exposure applied within this time period. the temperature dependence of the reactivation effect was also studied. the data obtained showed that the properties of recovery and protection against u.v. exposure are associated with the phy ... | 1976 | 956776 |
[changes in the polysaccharide biosynthesis of candida guilliermondii induced by diphenylamine]. | diphenylamine (dpa) inhibits the formation of glucan in candida guilliermondii while stimulating that of mannan. the total polysaccharide of cells decreases when growing in the presence of dpa. these effects are dependent on the concentration of dpa. compared with 14 structure-like substances, dpa was found to have the strongest activity. | 1976 | 969574 |
[detection in pichia guilliermondii of gtp-cyclohydrolase, an enzyme involved in the 1st stage of flavinogenesis]. | | 1976 | 976065 |
[localization of ubiquinone-9 in electron transport chain of candida guilliermondii]. | the activity of ubiquinone-dependent enzyme systems was studied in mitochondrial fractions of the yeast candida guilliermondii cultivated on the medium containing carbohydrates of hydrocarbons as a source of carbon. the activity of nadh2-oxidase and succinate-coq-reductase was higher in systems with mitochondrial fractions from the cells grown on the medium with n-paraffins than in mitochondrial fractions from the cells cultivated on the medium with glucose. about 30% of intracellular ubiquinone ... | 1976 | 1004242 |
bovine vesiculitis and epididymitis due to feed contamination by candida guilliermondii. | | 1976 | 1036341 |
[mechanism of retroinhibition in e regulation of flavinogenesis in yeasts of genus pichia]. | the kinetics of the synthesis of a riboflavin (rf) precursor, 2,6-dihydroxy-5-amino-4-ribitylaminopyrimidine (dharap), was studied using the washed cells of rf-deficient mutants of pichia guilliermondii r7g and pichia ohmeri r32 with blocked lumasine synthetase. rf inhibited the synthesis of dharap while cycloheximide in the absence of rf had no effect on this process. the data suggest that flavins regulate the biosynthesis of rf in p. guillier mondii and p. ohmeri b7 means of feed-back inhibiti ... | 1975 | 1160631 |
[nature of riboflavin precursors in pichia guilliermondi yeasts]. | thirty-nine riboflavin-deficient mutants have been isolated from three yeast strains of pichia guilliermondii (atss 9058, vkm y-1256, vkm y-1257) and f5-121 mutant which is capable of production of large amounts of riboflavin in the presence of iron in the medium. all mutants were divided into five groups according to the nature of precursors accumulated in the medium and growth reaction in media with 6,7-dimethyl-8-ribityllumasine and diacetyl. the mutants of the first group did not accumulate ... | 1975 | 1160636 |
[variability in the flavinogenic activity of pichia guilliermondi yeasts]. | the natural and induced variability of the flavinogenic activity was studied in the strain of pichia guilliermondii atcc 9058. the flavinogenic activity of the collection strain showed normal distribution; the amount of riboflavin(rf) accumulated in the medium differed several times in the extreme variants. in the clones with the maximum and minimum accumulation of rf, the distribution of the variants was asymmetric, due to the appearance of the cells with an average flavinogenic activity. the c ... | 1975 | 1160648 |
[protoplast formation and cell degradation of n-alkane-grown candida guilliermondii and candida spec]. | | 1975 | 1189471 |
[study of the regulation of the synthesis and activity of imp-dehydrogenase in the yeast pichia guilliermondii]. | activity of imp-dehydrogenase was studied in the cells of p. guilliermondii atcc 9058 from different growth phase; the yeast was cultivated in iron-rich and iron-deficient media. the highest activity of the enzyme was found in the iron-rich cells from the logarithmic growth phase. in the iron-deficient cells synthetizing great amounts of riboflavin the imp-dehydrogenase activity was somewhat higher in negative growth acceleration phase than that of iron-rich cells from the same stage. guanine co ... | 1975 | 1202696 |
[effect of urea and amino acids of the ornithine cycle on the biomass accumulation and amino acids synthesis by candida guilliermondii]. | when assimilating urea, arginine, ornithine and citrulline as the sole source of nitrogen, c. guilliermondii shows a higher economic coefficient of biomass accumulation (54.2, 59.7, 40.6% respectively) as compared with ammonium sulphate whose coefficient is 35.6%. nitrogen sources exert a significant influence on the content of essential amino acids in the alcohol soluble fraction of cell biomass. for instance, urea and arginine are responsible for the accumulation of ornithine (220 and 480 mug/ ... | 1975 | 1208387 |
[enzynatic method of disrupting yeast cell walls]. | the influence of the preparation of mucolytic enzymes obtained from the culture liquid filtrate of actinomyces griseinus on the yeast candida guilliermondii was investigated. lipids, hydrocarbons and proteins were extracted and free amino acids were separated from the biomass made of disrupted yeast cells. the extract contained 73% of cytoplasmic substances whereas normal yeast (enzymically untreated) contain on the average 13.7%. lipids and hydrocarbons were measured. | 1975 | 1208420 |
[effect of magnesium concentration in the culture medium on the trace element requirement of yeasts]. | the effect of different magnesium concentrations in the culture liquid (ranging from 2=6 to 220 mg/l) on the accumulation of mg and mn ions in the yeast candida guilliermondii was studied during their continuous cultivation on purified liquid paraffins. a reverse correlation between the accumulation of magnesium and manganese in the yeast biomass was established. the magnesium content in the biomass increased with an increase of its concentration in the nutrient medium. the biomass yield was opt ... | 1975 | 1208428 |
[flavinogenesis and regulation of purine biosynthesis de novo in pichia guilliermondi mutants resiatant to 8-azaguanine]. | 93 mutants resistant to 8-azaguanine (agr-mutants) were derived from the strain of pichia guilliermondii with blocked guanine deaminase (ec 3.5.4.3.) by uv-irradiation. the mutants retained the ability to uptake 8-azaguanine and guanine but could not deaminate guanine. some of the agr-mutants were found to accumulate large amounts of hypoxanthine and small amounts of guanine in the cultural medium. the inhibitory effect of guanine and 8-azaguanine but not adenine on the purine biosynthesis de no ... | 1975 | 1218707 |
[increased flavin synthesis in yeasts utilizing hydrocarbons]. | comparative studies of the process of possible overproduction of flavins by cultures with different flavinogenous activity grown on media with hydrocarbons and glucose have been carried out. the strains with a high flavinogenous activity, candida guilliermondii and torulopsis famata o-3, produced more flavins on media containing hydrocarbons than the cultures with a low flavinogenous activity. at a high content of iron in the medium, which is unfavourable for overproduction of riboflavin the rat ... | 1975 | 1239655 |
[participation of flavins and iron in regulating the rate of the 1st flavinogenesis reaction in pichia guilliermondii yeasts]. | | 1976 | 1248355 |
[effects of light shielding and reversible inactivation of candida guilliermondii, induced by 313 nm light]. | | 1976 | 1269401 |
demonstration of antibodies against candida guilliermondii var. guilliermondii in asymptomatic infertile men. | candida guilliermondii (c.g.) occurs mostly in animals, but it can affect humans too. using serological screening methods studies have been carried out in 57 (30 normozoospermic and 27 oligozoospermic) asymptomatic infertile men, of whom 15 (26.3%; 9 oligozoospermic and 6 normozoospermic) were found to have responded to candida guilliermondii var. guilliermondii. sperm parameters (cell count, motility, morphology) were determined and various tests (hypo-osmotic swelling test, bovine mucus penetr ... | 1992 | 1291877 |
in vitro susceptibility of 245 yeast isolates to amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole. | the in vitro activity of amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole was tested against 245 yeast strains isolated from clinical specimens (68 candida albicans, 74 candida tropicalis, 43 candida krusei, 28 candida glabrata, 19 candida parapsilosis, 8 candida lusitaniae and 5 candida guilliermondii). an agar dilution method was employed to carry out testing. minimal inhibitory concentrations to restrain 90% of isolate growth (mic90) ranged from 0.12 to 2 mg/l for ... | 1992 | 1337508 |
colony morphotype on sabouraud-triphenyltetrazolium agar: a simple and inexpensive method for candida subspecies discrimination. | a new method of candida subspecies discrimination on sabouraud-triphenyltetrazolium agar is reported. five hundred sixty-two strains of candida and torulopsis glabrata, previously identified by conventional mycological methods, were studied. each strain received a three-letter code and a number based on its colonial morphology. sixteen morphotypes were found for candida albicans, 6 were found for candida parapsilosis, 4 were found for both candida guilliermondii and candida krusei, and 12 were f ... | 1992 | 1400981 |
[analysis of 84 cases with fungemia]. | eighty-four patients with fungemia were analyzed. fungi had been isolated by culture of blood samples, including blood from the catheter for intravenous hyperalimentation, between 1986-1990. candida albicans (39.3%), candida parapsilosis (20.2%), candida tropicalis (11.9%), candida glabrata (10.7%), candida guilliermondii (4.8%) and trichosporon beigelii (4.8%) were the most frequently isolated fungal pathogens. four patients' blood yielded two different fungal species. fifty-nine cases were mal ... | 1992 | 1402094 |
detection of serum candida antigens by enzyme-linked immunosorbent assay and a latex agglutination test with anti-candida albicans and anti-candida krusei antibodies. | serum samples from 197 patients with and without candidiasis were assayed for candida albicans mannan and candida krusei mannan by an enzyme-linked immunosorbent assay (elisa) and a latex agglutination test (la) and for d-arabinitol by the enzymatic fluorometric method. of the 43 patients positive for c. albicans mannan (> or = 0.2 ng/ml), 34 were infected with c. albicans and 9 were infected with candida tropicalis. c. krusei mannan (> or = 0.3 ng/ml) was detected in 10 patients infected with c ... | 1992 | 1452696 |
selection of strains capable of utilizing d-xylose and cellobiose to produce ethanol by electric field-induced protoplast fusion. | the protoplast fusion between candida guilliermondii s208 (arg-) and saccharomyces cerevisiae 314 was induced by three pulses (18kv/cm, 10 microseconds duration) applied at an interval of 1 s with the model gh-401 electric induced gene transfer/cell fusion system. the frequency of appearance of prototrophic hybrids was 3.6 x 10(-3). stable fusion products were obtained after continual subculture over 20 times on selected and unselected media. comparative studies showed that the fusion products w ... | 1992 | 1457722 |
mitotically unstable polyploids in the yeast pichia guilliermondii. | attempts to obtain triploids or tetraploids of p. guilliermondii by sexual hybridization led to mitotically stable hybrids. however, their dna content per cell was not higher than in diploids. the results of random spore analysis demonstrate that these hybrids were in fact aneuploids which obviously suffered drastic chromosome losses immediately after mating. this phenomenon could have been caused either by aneuploidy already present in the parental strains or it might have been due to a general ... | 1992 | 1487815 |
physiological study on riboflavin production by hydrocarbon-utilizing candida guilliermondii wickerham. | the production of riboflavin (vitamin b2) by candida guilliermondii wickerham cultivated on solar-containing medium was stimulated in the presence of corn oil (0.1 g%), arginine, phenylalanine (1 m mole/l) or coso4.7h2o (1000 micrograms/l). on the other hand, emulsifying agents strongly inhibited yeast growth and vitamin production. similarly, feso4.7h2o and mnso4.4h2o at 50 and 200 micrograms/l levels, respectively, also showed inhibitory effect. among the tested purines, xanthine enhanced vita ... | 1992 | 1609556 |
headspace flavour compounds produced by yeasts and lactobacilli during fermentation of preferments and bread doughs. | production of volatile flavour compounds during fermentation with pure cultures of saccharomyces cerevisiae and candida guilliermondii, lactobacillus brevis and lactobacillus plantarum have been investigated, using wheat doughs and several preferements as substrates. for yeast, preferments consisted of 10% (w/v) glucose, maltose and sucrose solutions, whereas for lactobacilli they consisted of supplemented and unsupplemented (3% and 10% (w/v)) glucose solutions, and a 10% (w/v) wheat flour slurr ... | 1992 | 1622751 |
[in vitro antifungal activity of itraconazole, a new triazole antifungal agent, against clinical isolates from patients with systemic mycoses]. | the in vitro antifungal activity of itraconazole (itz), a new oral triazole antifungal agent, against clinical isolates from patients with systemic mycoses were compared with those of existing systemic antifungals, viz. ketoconazole (kcz), miconazole or amphotericin b. the studies were performed with 65 isolates of pathogenic yeasts and 13 isolates of aspergillus spp. using the agar dilution method on casitone agar. itz showed the most potent antifungal activities against isolates of pathogenic ... | 1991 | 1652654 |
pseudooutbreak of candida guilliermondii fungemia in a neonatal intensive care unit. | during a 3-week period multiple blood cultures obtained from 14 neonatal intensive care unit infants and 3 newborn unit babies grew candida guilliermondii, a yeast rarely associated with infections in humans. at the time of detection of positive cultures, most infants had been hospitalized for days or weeks for serious perinatal conditions and treated with antibiotics and intravenous hyperalimentation. two critically ill premature infants from whom the yeast was isolated were given amphotericin ... | 1991 | 1766709 |
biosorption of copper by yeasts. | the ability to accumulate copper from aqueous solutions was determined with different yeast species. yeast cells did not show any significant differences in process kinetics. the uptake was very fast and was influenced by environmental factors. the metal-accumulating capacity differed among the tested strains. the yeast candida tropicalis and pichia guilliermondii were chosen for extensive research. cells of the stationary growth phase were able to adsorb a high amount of copper. the uptake capa ... | 1991 | 1777357 |
long-term laboratory preservation of pathogenic yeasts in water. | a total of 1583 yeast isolates, mostly candida albicans, has been maintained under sterile distilled water for periods ranging from 1 to 18 years. overall, 71 (4.5%) of the isolates were not recoverable at 37 degrees c. survival of the yeasts was 97% in the first 5 years and 96% after 10 years. isolates of candida krusei and saccharomyces cerevisiae were particularly unlikely to survive storage in water and these species should be preserved by other means if they are to be kept for more than a f ... | 1991 | 1815033 |
near-uv activation of yeast growth. | near-uv radiation (337 nm) at fluences of 2-10 kj m-2 activates, during the lag-phase, subsequent growth of the yeast candida guilliermondii. photostimulation occurs only after a temperature-dependent time interval (1-2 h) between short-period irradiation and the onset of cell growth on a nutrient medium. a photoactivated enzymatic synthesis of an intermediate metabolite regulating cell growth is proposed as the cause of the biochemical changes responsible for the photostimulatory effect. on the ... | 1991 | 1835498 |
in vitro susceptibility of 119 yeast isolates to fluconazole, 5-fluorocytosine, amphotericin b and ketoconazole. | the in vitro activity of fluconazole was tested against 13 yeast species (119 strains) isolated from clinical specimens during a 3-month period. for comparative purposes, three other antifungal compounds (5-fluorocytosine, amphotericin b and ketoconazole) were also tested. the tests were carried out using a microautomated method previously developed in our laboratory. the method allowed us to determine the minimum inhibitory concentration (mic) of the four antifungal drugs used. for each of the ... | 1991 | 2013239 |
fermentation and aerobic metabolism of cellodextrins by yeasts. | the fermentation and aerobic metabolism of cellodextrins by 14 yeast species or strains was monitored. when grown aerobically, candida wickerhamii, c. guilliermondii, and c. molischiana metabolized cellodextrins of degree of polymerization 3 to 6. c. wickerhamii and c. molischiana also fermented these substrates, while c. guilliermondii fermented only cellodextrins of degree of polymerization less than or equal to 3. debaryomyces polymorphus, pichia guilliermondii, clavispora lusitaniae, and one ... | 1991 | 2039228 |
regulation of chorismate mutase activity of various yeast species by aromatic amino acids. | the regulatory properties of chorismate mutase, its cellular localization and isoenzyme pattern were investigated in 23 yeast species. all yeasts contained only a single form of the enzyme, which is localized exclusively in the cytosol. the enzyme activity from all sources was activated 3-(rhodotorula aurantiaca) to 185-fold (candida maltosa) by tryptophan. the tryptophan concentration, which was necessary to obtain half maximum velocity was determined to be between 2 (pichia guilliermondii) and ... | 1991 | 2059012 |
[comparative studies of the growth behavior of wild strains and fusion hybrids of pichia guilliermondii in discontinuous and continuous culture]. | the influence of several temperatures on the wild strain pichia guilliermondii pg 0799 and a selected fusion product fp 3/9 was studied in batch- and continuous cultures. growth and substrate utilization of both strains were compared by determination of specific growth rates, yield coefficients and biomass production. the results showed, that strain fp 3/9 has in general a higher optimum of temperature for biomass production. | 1991 | 2068871 |
suppurative oral candidosis. review of the literature and report of a case. | a case of a dento-alveolar abscess due to a polymicrobial infection with a yeast, candida guilliermondii and two other oral bacteria are described. a review of the literature has revealed only a single case of an odontogenic abscess due to candida species and we believe this to be the first report of a dentoalveolar infection where candida guilliermondii is associated as a co-pathogen. | 1990 | 2124592 |
discriminant analysis of cellular fatty acids of candida species, torulopsis glabrata, and cryptococcus neoformans determined by gas-liquid chromatography. | we used discriminant analysis of cellular fatty acid compositions determined by gas-liquid chromatography to differentiate yeastlike fungi (a total of 190 strains; including 37 candida albicans strains, 21 candida krusei strains, 13 candida guilliermondii strains, 37 candida tropicalis strains, 10 candida pseudotropicalis strains, 24 candida parapsilosis strains, 32 torulopsis glabrata strains, and 16 cryptococcus neoformans strains). previous results with a standard strain of c. albicans indica ... | 1990 | 2199515 |
studies on the 4-carbon precursor in the biosynthesis of riboflavin. purification and properties of l-3,4-dihydroxy-2-butanone-4-phosphate synthase. | the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1h,3h)-pyrimidinedione requires a phosphorylated 4-carbon intermediate which has been designated as compound x (neuberger, g., and bacher, a. (1985) biochem. biophys. res. commun. 127, 175-181). the enzyme catalyzing the formation of compound x has been purified about 600-fold from the cell extract of the flavinogenic yeast candida guilliermondii by chromatographic procedures. the purified ... | 1990 | 2246238 |