| [lactate oxidation by hansenula anomala cells]. | lactate oxidation by freeze-dried cells of hansenula anomala was studied in the presence of exogenous electron acceptors, potassium ferricyanide (pf) and phenazine metosulfate (pms). the product of oxidation is pyruvate. the apparent michaelis constants for lactate, pf and pms are 1.2, 0.6 and 0.05, respectively. the ph optimum of lactate oxidation is 8.0. the freeze-dried cells of the yeast are inactivated by 5 mm bromopyruvate by 50% within 1.5 min. treatment of the intact cells with ultrasoun ... | 1979 | 39226 |
| [some patterns of inosinic acid precursor accumulation by purine-dependent mutants of candida pelliculosa]. | | 1976 | 967050 |
| transient fungaemia due to candida pelliculosa in a patient with aids. | candida pelliculosa redaelli (syn. hansenula anomala) was isolated from blood cultures of a patient with acquired immunodeficiency syndrome. the transient candidaemia was apparently associated with intravenous drug addiction. the isolate was identified by standard methods such as api 20 c aux and 16 discs carbon auxanogram. the isolate was sensitive to amphotericin b, 5-fluorocytosine and ketoconazole. this organism adds to the growing list of candida species associated with acquired immunodefic ... | 1991 | 1803237 |
| [hansenula anomala infection in acute leukemia]. | hansenula anomala infection is a clinical rarity which has only been reported in 21 cases. although most reported cases had one or more of the risk factors of fungemia, only three developed in hematological patients. in the present study we report two cases of hansenula anomala infection in acute leukemia, one in its teleomorphic form and another in the asexual form of this yeast, candida pelliculosa. the sustained chemotherapy and steroid and antibiotic treatment were some of the risk factors o ... | 1990 | 2081172 |
| hansenula anomala infections in children: from asymptomatic colonization to tissue invasion. | | 1991 | 2067890 |
| electron-transfer steps involved in the reactivity of hansenula anomala flavocytochrome b2 as deduced from deuterium isotope effects and simulation studies. | the l-lactate-flavocytochrome b2-ferricyanide electron-transfer system from the yeast hansenula anomala was investigated by rapid-reaction techniques. the kinetics of reduction of oxidized flavocytochrome b2 by l-lactate and l-[2h]lactate were biphasic both for flavin and haem prosthetic groups and at all concentrations tested. the first-order rate constants of the rapid and slow phases depended upon substrate concentrations, a saturation behaviour being exhibited. substitution of the c alpha-h ... | 1991 | 2001234 |
| 'antibiobodies': antibiotic-like anti-idiotypic antibodies. | pathogenic micro-organisms such as candida albicans may be susceptible to the activity of antimicrobial products like yeast killer toxins due to the presence of specific cell wall receptors for these agents. anti-idiotypic antibodies (anti-ids) were produced that competed for these receptors with the yeast killer toxin of a strain of pichia anomala. we report here that affinity chromatography purified anti-ids may kill c. albicans cells in vitro which are susceptible to the activity of the yeast ... | 1991 | 1941431 |
| differential toxinogenesis in the genus pichia detected by an anti-yeast killer toxin monoclonal antibody. | the differential toxinogenesis of 25 isolates belonging to species of the potential yeast killer genus pichia that were previously classified in the genus hansenula was comparatively demonstrated by two serologic techniques (indirect immunofluorescence and double immunodiffusion) by using a monoclonal antibody against a yeast killer toxin produced by a selected strain of pichia anomala (ucsc 25f). the killer phenotypes of the pichia isolates were evaluated by their ability to kill each other. th ... | 1991 | 1907819 |
| molecular cloning of cdna for antimycin a-inducible mrna and its role in cyanide-resistant respiration in hansenula anomala. | a cdna for mrna induced by antimycin a in hansenula anomala was cloned. the mrna for the cdna was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. the nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cdna. an antibody recognizing the protein encoded by the open reading frame was produced. immunoblotting of h. anomala proteins with this antibody showed that a 36 kda pr ... | 1991 | 1883836 |
| bioconversion of quininone to quinidine by resting cells of hansenula anomala var. schneggii. | | 1991 | 1874545 |
| immobilization of yeast and the function of immobilized yeast. | hansenula anomala, which catalyzes an asymmetric reduction, was immobilized in bulk or spherical crosslinked polymers. the catalytic activity of the yeast for enantio-selective reduction of 3,8-dioxo-4-methoxycarbonyl-9-methyl-delta 4(10)-octalin (lab) was severely affected by the immobilization conditions, such as the crosslink density, and by hydrophobicity of polymers and other components used for immobilization, oxygen concentration in the medium, etc. in some immobilized systems, the ratio ... | 1991 | 1799952 |
| hansenula anomala fungemia in an infant with gastric and cardiac complications with a review of the literature. | a 6-month-old female infant, with a birth weight of 2.74 kilograms, was born with multiple congenital abnormalities, including gastric and gastrointestinal defects. she was admitted to the hospital with hematemesis. the patient could not be fed orally, and parenteral nutrition was initiated through a central venous catheter. following pyloroplasty, she developed superior vena cava syndrome, renal disfunction and episodes of sepsis. stool and respiratory specimens were negative for fungi, but fou ... | 1992 | 1644153 |
| yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops. | the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ... | 1990 | 2321936 |
| laser flash photolysis study of the kinetics of electron transfer reactions of flavocytochrome b2 from hansenula anomala: further evidence for intramolecular electron transfer mediated by ligand binding. | intramolecular electron transfer between the heme and flavin cofactors of flavocytochrome b2 is an obligatory step during the enzymatic oxidation of l-lactate and subsequent reduction of cytochrome c. previous kinetic studies using both steady-state and transient methods have suggested that such intramolecular electron transfer is inhibited when pyruvate, the two-electron oxidation product of l-lactate, is bound at the active site of hansenula anomala flavocytochrome b2. in contrast to this, we ... | 1992 | 1547219 |
| occurrence of the general control of amino acid biosynthesis in yeasts. | the response of three amino acid biosynthetic enzymes, threonine dehydratase, tyrosine aminotransferase and saccharopine dehydrogenase, to conditions of histidine, tryptophan or lysine limitation was investigated in 15 yeast species. the activities of all these enzymes increased about two- to fourfold as a result of action of the general control of amino acid biosynthesis in brettanomyces anomalus, candida maltosa, hansenula polymorpha, rhodosporidium toruloides, saccharomyces cerevisiae and yar ... | 1990 | 2338623 |
| effect of nitrogen source on the levels of nitrate reductase in the yeast hansenula anomala. | levels of nitrate reductase (nr) protein in hansenula anomala and hansenula wingei were determined using specific antiserum raised against the enzyme from h. anomala. extracts from nitrate-grown cells contained nr protein, while in those from cells grown on ammonium, glutamine or peptone, no cross-reacting material could be observed. enzyme activity correlated with the levels of cross-reacting material. when nitrate was used as nitrogen source, nr was always present, even in cultures with ammoni ... | 1992 | 1512576 |
| ultrastructural immunodetection of a pichia anomala killer toxin: a preliminary study. | a monoclonal antibody (mab kt4), produced against a pichia anomala killer toxin, was used to study the secretion process of toxin producing cells. the indirect immunofluorescence assay, performed with large concentrations of mab kt4, showed a homogeneous distribution of the epitope at the cell surface of the p anomala cells. when increasing dilutions of mab kt4 were employed, a 'punctuated' labeling appeared on the yeast's cell wall which suggested a heterogeneous secretion of the killer toxin. ... | 1992 | 1381254 |
| studies on the enzymatic reduction of n-boc-4s-amino-3-oxo-5-phenylpentanoic acid methylester. | the enzymatic reduction of n-boc-4s-amino-3-oxo-5-phenylpentanoic acid methylester, the key intermediate in the stereoselective synthesis of a statinanalogue, was studied with hansenula anomala and hansenula silvicola. using whole cells of h. anomala gives complete conversion and a diastereomeric excess of 88% of the desired 3s, 4s statinanalogue. the strain contains two nadph-dependent oxidoreductases, that can be separated by ion exchange chromatography or gelfiltration, yielding the 3s, 4s or ... | 1992 | 1369149 |
| [presence of pathogenic yeasts in the feces of the semi-domesticated pigeon (columba livia, gmelin 1789, urban type) from the city of turin]. | the results of a survey on the presence of pathogenic yeasts in pigeon droppings collected in turin, are shown. the study was carried out in 8 densely populated areas, where human-animal contact is highest. a total of 427 pigeon dropping samples, most of which fresh, were collected. 550 yeast colonies, clinically interesting or at least pathologically significant, were isolated by the identification routine methods. the yeasts belong to the following species: candida albicans, c. humicola, c. kr ... | 1989 | 2487001 |
| a yeast biosensor for glucose determination. | a yeast potentiometric biosensor for glucose determination is described. after induction of glycolytic enzyme synthesis a cell suspension of the yeast hansenula anomala is retained in calcium alginate gel on the surface of a glass electrode. this biosensor gives a nernstian response in glucose concentration of 5 x 10(-4)-5 x 10(-3) mol/l with a response time of 5 min and a life-time of at least 2 months. mannose and fructose are the only significantly interfering substances. the biosensor was us ... | 1991 | 1367229 |
| fungemia caused by hansenula anomala: successful treatment with fluconazole. | fungemia, due to hansenula anomala, developed in an adult patient with small cell lung cancer who received anti-cancer chemotherapy and plasmapheresis for a sensori-motor neuropathy complication. treatment with intravenous infusion of fluconazole in addition to the removal of the central venous catheter was successful in treating the fungemia. pathogenic hansenula anomala infections are rare, but reports of this infection have been increasing. the use of fluconazole treatment for this infection ... | 1992 | 1324035 |
| possible involvement of superoxide anion in the induction of cyanide-resistant respiration in hansenula anomala. | a chemiluminescence study showed that qi site inhibitors such as antimycin a induce o2- generation in respiring cyanide-sensitive mitochondria from the yeast, hansenula anomala. the o2- generation was suppressed by radical scavengers such as flavone, butylated hydroxyanisole, and co0. induction of cyanide-resistant respiration in h. anomala cells by qi site inhibitors was also inhibited by these radical scavengers. furthermore, antimycin a-induced synthesis of the mitochondrial 36-kda protein, w ... | 1992 | 1318225 |
| fungaemia due to candida pelliculosa in a case of acute pancreatitis. | we describe a case of fungaemia due to candida pelliculosa (teleomorph: hansenula anomala) in an otherwise non-immunocompromised patient with acute necrotizing pancreatitis of unknown origin. this species of candida should be added to the list of pathogenic fungi which are increasingly important not only in patients with underlying immunosuppressive disease but also in patients with, for instance, severe surgical illness. | 1992 | 1302804 |
| comparison between the yeast flora of middle eastern and western european soft drinks. | samples of a carbonated orange drink, raw materials, and intermediate products originating from 6 iraqi bottling plants were examined. 69 drinks, 4 flavoured syrups and 19 simple syrups contained yeasts, whereas all samples from one plant and all samples of beverage base were free from viable yeasts. from the orange drink 2 species were isolated viz. saccharomyces montanus and torulopsis stellata. the following species were present in simple syrup: hansenula anomala, sacch. bisporus var. mellis, ... | 1976 | 1087865 |
| lipids of the yeast hansenula anomala. | an analysis of the free lipids of hansenula anomala was performed. the main fatty acids obtained by saponification of whole cell crude lipids were palmitic, c18:1, c18:2 and c18:3 acids. in mitochondrial lipids the tri-unsaturated acid was present as traces. fatty acid composition of each class of lipids was also determined. phosphatidylcholine and phosphatidylethanolamine were the main phospholipids; phosphatidylserine, phosphatidylinositol and cardiolipin were also characterized. the most abun ... | 1977 | 870084 |
| haematological consequences of feeding trout with a single cell protein (hansenula anomala) [proceedings]. | | 1978 | 733780 |
| a standard high-yield purification of l-(+)-lactate: cytochrome c oxidoreductase (cytochrome b2) from the yeast hansenula anomala. | | 1978 | 697031 |
| [effect of raised temperatures on the protein and rna synthesis rate in yeasts]. | the rates of incorporation of labelled precursors of protein (14c-dl-leucine) and rna (14c-uracil) into the cells of synchronous yeast cultures of pichia membranaefaciens, hansenula anomala, saccharomyces cerevisiae and saccharomyces (fabospora) fragilis were studied at different temperatures. synthesis of rna and then of protein was inhibited in h. anomala if the temperature was increased above the optimal one. this is manifested even more distinctly in sacch. cerevisiae and p. membranaefaciens ... | 1979 | 370520 |
| structural basis for the kinetic differences between flavocytochromes b2 from the yeasts hansenula anomala and saccharomyces cerevisiae. | to understand the structural basis for the different catalytic behaviour of the flavocytochromes b2 from saccharomyces cerevisiae and hansenula anomala we have cloned and sequenced the gene encoding the latter. we have compared the amino acid sequences of the mature proteins in the context of the known crystal structure of s. cerevisiae flavocytochrome b2. overall there is 60% sequence identity, but two surface loops in particular are strikingly different in primary structure and net charge. | 1989 | 2688640 |
| yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil. | samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ... | 1989 | 2750315 |
| the association--dissociation states of subunits in the l (+) lactate cytochrome c oxidoreductases (cytochromes b2) extracted from saccharomyces cerevisiae and hansenula anomala yeasts. | | 1978 | 348238 |
| [complexes between l (+) lactate: cytochrome c oxidoreductases from the yeasts saccharomyces cerevisiae or hansenula anomala and horse heart cytochrome c]. | | 1977 | 200281 |
| infection and colonisation of neonates by hansenula anomala. | over a 13-month period 52 neonates (10% of those admitted to the mersey regional neonatal intensive care unit) were found to be colonised with the ascosporagenous yeast hansenula anomala. 8 babies became infected, all but 1 of whom were heavily colonised before infection. 7 of the 8 infected babies were of very low birth-weight (less than 1500 g). all 8 had multiple problems associated with low birth-weight and prematurity and were kept in the intensive care unit. 5 babies had fungaemia, 2 had f ... | 1986 | 2868164 |
| catheter-related fungemia by hansenula anomala. | | 1989 | 2919947 |
| the effect of papulacandin b on (1----3)-beta-d-glucan synthetases. a possible relationship between inhibition and enzyme conformation. | the antibiotic, papulacandin b, inhibited growth or (1----3)-beta-d-glucan synthetase (or both) in the fungi saccharomyces cerevisiae, hansenula anomala, neurospora crassa, cryptococcus laurentii, schizophyllum commune and wangiella dermatitidis. no effect was observed on achlya ambisexualis. there was no apparent correlation between the inhibition of growth and that of the synthetase. with most of the fungal extracts, the inhibition of glucan synthetase by papulacandin b became less pronounced ... | 1986 | 2942248 |
| regulation of fungal cell wall growth: a guanine nucleotide-binding, proteinaceous component required for activity of (1----3)-beta-d-glucan synthase. | by treatment with detergent and nacl, particulate (1----3)-beta-d-glucan synthase (ec 2.4.1.34) from hansenula anomala or neurospora crassa was dissociated into a "soluble fraction" and a "membrane fraction." each fraction alone was almost inactive, but enzymatic activity could be reconstituted by mixing the two fractions and adding gtp or one of its analogs. based on their lability to heat and to incubation with trypsin, the activity in both fractions is proteinaceous. the active component in t ... | 1986 | 2942941 |
| proteolysis of l-(+)-lactate cytochrome c oxidoreductase (cytochrome b2) extracted from saccharomyces cerevisiae and hansenula anomala yeasts. | the l-(+)-lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts saccharomyces cerevisiae and hansenula anomala were partially hydrolysed in various concentrations of trypsin. conditions were found which allowed the isolation from the hansenula enzyme of a 140 000 +/- 10 000-dalton flavoprotein. the prosthetic flavin groups were still reducible by substrate (spectroscopic evidence) but the flavoprotein was unable to form a complex with cytochrome c, the physiological acceptor in th ... | 1977 | 195807 |
| essential role of ferrous iron in cyanide-resistant respiration in hansenula anomala. | antimycin a-dependent induction of cyanide-resistant respiration in hansenula anomala was completely blocked by o-phenanthroline, alpha,alpha'-dipyridyl, or 8-hydroxyquinoline. pulse-labeling of the cells with [35s]methionine in the presence of both antimycin a and o-phenanthroline indicated that the 36-kda protein previously reported to be involved in cyanide-resistant respiration [(1989) j. biochem. 105, 864-866] was formed in mitochondria even under these conditions. the addition of fe2+, but ... | 1990 | 2114321 |
| hydrophobic cluster analysis: procedures to derive structural and functional information from 2-d-representation of protein sequences. | hydrophobic cluster analysis (hca) [15] is a very efficient method to analyse and compare protein sequences. despite its effectiveness, this method is not widely used because it relies in part on the experience and training of the user. in this article, detailed guidelines as to the use of hca are presented and include discussions on: the definition of the hydrophobic clusters and their relationships with secondary and tertiary structures; the length of the clusters; the amino acid classificatio ... | 1990 | 2126461 |
| cloning of candida pelliculosa beta-glucosidase gene and its expression in saccharomyces cerevisiae. | candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. from a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a s. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as an indicator. it was proved by southern analysis that the dna fragment carrying the beta-glucosidase gene originated from c. pelliculosa. beta-glucosidase produced by s. cerevisiae transformants was se ... | 1986 | 3012284 |
| immunochemical and structural analysis of the cell wall mannan as the basis of the taxonomic reidentification of a yeast strain. | the immunochemical properties and structural features of d-mannans from two candida parapsilosis strains were studied. weak cross-reactivity of d-mannans with antisera produced to c. parapsilosis strains was observed, as well as significant differences in the mannans structures were found by means of methylation analysis, acetolysis and 1h-nmr spectroscopy. in view of the discrepancies found, new taxonomic identification of one strain was carried out. on the basis of assimilation of potassium ni ... | 1990 | 2129042 |
| detection by immunofluorescent anti-idiotypic antibodies of yeast killer toxin cell wall receptors of candida albicans. | yeast killer toxin cell wall receptors of candida albicans were observed by indirect immunofluorescence using an affinity purified rabbit anti-idiotypic antiserum. the antiserum had been raised against a monoclonal antibody neutralizing the in vitro activity of a killer toxin produced by a selected strain of hansenula anomala ucsc 25f. this simple procedure permitted the location of killer toxin cell wall receptors in various morphological phases of the yeast cells. the use of the indirect immun ... | 1990 | 2170534 |
| involvement of a cell wall receptor in the mode of action of an anti-candida toxin of pichia anomala. | hanes-woolf, dixon, and hill plots of growth rates of candida albicans rc1 grown in various concentrations of glucose and a pichia anomala wc65 toxin suggested the presence of toxin-binding sites. indirect immunofluorescence microscopy with antitoxin antibodies demonstrated binding of the toxin to the cell wall. resistance to the toxin of a mutant saccharomyces cerevisiae deficient in cell wall beta-1-6-d-glucan suggests that the glucan either served as the receptor or influenced the number or c ... | 1990 | 2201251 |
| production of yeast killer toxin in experimentally infected animals. | the ability of a killer yeast (pichia anomala, ucsc 25f) to produce toxin in vivo was demonstrated, for the first time, in tissues of normal and immunosuppressed experimentally infected mice by means of a fluorescent antibody technique and a killer toxin specific monoclonal antibody. the possible significance of the findings is discussed. | 1990 | 2201918 |
| a 13c comparative nuclear magnetic resonance study of organic solute production and excretion by the yeasts hansenula anomala and saccharomyces cerevisiae in saline media. | glycerol, arabitol and trehalose were the principle solutes detected in cellular extracts of hansenula anomala, using natural-abundance 13c nuclear magnetic resonance spectroscopy. only the two polyols accumulated in response to increased salinity, glycerol increase being far greater. arabitol content also increased with culture age, independently of the presence or absence of salt and in line with the evolution of trehalose content. glycerol retention potential was 15 times greater for hansenul ... | 1988 | 3061619 |
| urinary tract infection caused by hansenula anomala. | a case of urinary tract infection due to hansenula anomala is reported. the infection occurred in a cadaver kidney transplant patient who was receiving immuno-suppression therapy. survey of the literature revealed that human infections due to this organism are rare and its causal relationship in urinary tract infection has not been previously reported. | 1988 | 3065645 |
| characterization of cyanide-resistant respiration and appearance of a 36 kda protein in mitochondria isolated from antimycin a-treated hansenula anomala. | mitochondria exhibiting cyanide-resistant respiration were isolated from hansenula anomala which had been incubated in the presence of antimycin a to induce cyanide-resistant respiration. the cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin a or myxothiazol, suggesting that the branching of the pathway from the normal cyanide-sensitive pathway takes place at the coenzyme q level. analysis of mitochondrial proteins by sodium dodecyl sulfate gel electrophoresis ... | 1990 | 2229020 |
| stimulation of beta(1----3)glucan synthetase of various fungi by nucleoside triphosphates: generalized regulatory mechanism for cell wall biosynthesis. | particulate fractions from the taxonomically diverse fungi achlya ambisexualis, hansenula anomala, neurospora crassa, cryptococcus laurentii, schizophyllum commune, and wangiella dermatitidis were found to catalyze the time-dependent incorporation of glucose from udp-[14c]glucose into a water-insoluble material. the reaction was stimulated by bovine serum albumin. the product was characterized as beta(1----3)glucan on the basis of its resistance to alpha- and beta-amylase and susceptibility to b ... | 1985 | 3156122 |
| evaluation of the updated vitek yeast identification data base. | using 398 isolates of yeasts and yeastlike fungi comprising 9 genera and 26 species, as well as the hyphomycete geotrichum candidum and the achlorophyllous alga prototheca wickerhamii, we compared the api 20c yeast identification system with the modified vitek yeast identification system with an expanded data base. we found 11 discrepancies between the two systems: five (1.3%) of the isolates (blastoschizomyces capitatus, 1; candida albicans, 1; hansenula anomala, 1; rhodotorula minuta, 2) had b ... | 1990 | 2229376 |
| a 36-kda mitochondrial protein is responsible for cyanide-resistant respiration in hansenula anomala. | antimycin a-dependent induction of cyanide-resistant respiration in hansenula anomala was reversibly blocked by carbonylcyanide-m-chlorophenylhydrazone (cccp). when the cells were pulse-labeled with [35s]methionine in the presence of both antimycin a and cccp, the radioactivity was incorporated into a 39 kda mitochondrial protein. upon removal of cccp, this protein was processed into a 36 kda form. the increase in the 36 kda protein completely paralleled that in cyanide-resistant respiration act ... | 1990 | 2338139 |
| transport of malic acid and other dicarboxylic acids in the yeast hansenula anomala. | dl-malic acid-grown cells of the yeast hansenula anomala formed a saturable transport system that mediated accumulative transport of l-malic acid with the following kinetic parameters at ph 5.0: vmax, 0.20 nmol.s-1.mg (dry weight)-1; km, 0.076 mm l-malate. uptake of malic acid was accompanied by proton disappearance from the external medium with rates that followed michaelis-menten kinetics as a function of malic acid concentration. fumaric acid, alpha-ketoglutaric acid, oxaloacetic acid, d-mali ... | 1990 | 2339872 |
| production and characterization of yeast killer toxin monoclonal antibodies. | monoclonal antibodies were obtained after fusion of mouse myeloma cells with spleen cells isolated from mice primed with a crude extract of yeast killer toxin produced by a strain of hansenula anomala. hybridomas were selected by specific immunoassay reaction of their fluid with crude yeast killer toxin extract. among the monoclonal antibodies, which were characterized by the western blot technique, one (designated kt4) proved to have precipitating properties, thus permitting the neutralization ... | 1987 | 2434524 |
| fungemia due to hansenula anomala. | | 1986 | 3466066 |
| bod measurement system with flowthrough electrode. | biochemical oxygen demand (bod) is one of the most important and widely used parameters in measurement of organic pollution. bod determination by the conventional method requires five days and is too protracted and complicated for use in process control. we constructed a microbial sensor for bod estimation consisting of immobilized yeast cells (hansenula anomala) and an oxygen electrode. the electrode was carefully designed and a flowthrough measuring system constructed. a biosensor response tim ... | 1989 | 2491327 |
| hansenula anomala infection. | | 1989 | 2501853 |
| serological study of yeast killer toxins by monoclonal antibodies. | yeast killer toxins coded by determined and undetermined killer plasmids or presumptive nuclear gene(s) in various genera (saccharomyces, kluyveromyces, pichia and candida) have been serologically investigated by a monoclonal antibody (kt4), produced against the yeast killer toxin of pichia (hansenula) anomala ucsc 25f. double immunodiffusion with the killer toxins as antigens and indirect immunofluorescence on whole cells of the corresponding killer yeast have been used. in both the serological ... | 1989 | 2515441 |
| purification and characterization of the anti-candida toxin of pichia anomala wc 65. | pichia anomala wc 65 secretes a toxin that is inhibitory to a variety of yeasts, including strains of the animal pathogen candida albicans. the toxin was purified to homogeneity by ultrafiltration, ethanol precipitation, ion-exchange chromatography with a mono q column, and gel permeation chromatography with a superose 12 column. the toxin had a molecular weight of 83,300 as determined by electrophoresis on sodium dodecyl sulfate-polyacrylamide gradient gels and a molecular weight of 85,290 as d ... | 1989 | 2653213 |
| potential therapeutic effect of yeast killer toxin. | experimental infections were produced in guinea pigs, rabbits and dogs with lesions similar to those seen in human seborrheic dermatitis and otitis externa by cutaneous application of cultures of malassezia furfur and m. pachydermatis. infected animals were treated by topical application of a concentrated yeast killer toxin (hansenula anomala ucsc 25f). clinical recovery as well as negative mycological test cultures of infected animals proved to the clearly associated with the treatment by the k ... | 1986 | 3796712 |
| regulation of dehydrogenases/one-electron transferases by modification of flavin redox potentials. effect of product binding on semiquinone stabilization in yeast flavocytochrome b2. | spectroscopic and potentiometric measurements have been carried out, at room temperature, during anaerobic titrations of hansenula anomala l-lactate cytochrome c oxidoreductase (or flavocytochrome b2) both in the presence and in the absence of pyruvate (the physiological reaction product). under the same conditions, the flavin spectral contribution was estimated and the flavosemiquinone proportion was directly determined by electron paramagnetic resonance measurements. in the present study, we s ... | 1986 | 3007132 |
| pulse radiolysis study of a yeast cytochrome c from hansenula anomala. | the reduction of hansenula anomala yeast cytochrome c by e-aq and co-.2 was investigated by pulse radiolysis, at a high reductant to protein concentration ratio. the reactivity of the radicals was studied by observing absorbance changes in the cytochrome c spectrum over the wavelength range 280-600 nm. at ph 7, over the time scale of the radical decays (i.e. 0-4 microseconds for e-aq; 0-40 microseconds for co-.2s) and beyond, the hemoprotein was reduced without any spectrally detected intermedia ... | 1986 | 3015259 |
| comparison of the processes involved in reduction by the substrate for two homologous flavocytochromes b2 from different species of yeast. | a detailed study of the electron exchanges involved between fmn and haem b2 groups within flavocytochrome b2 of yeast hansenula anomala (h-enzyme) was performed. the results were compared with those for the homologous enzyme of yeast saccharomyces cerevisiae (sx-enzyme) re-investigated at 5 degrees c. the mid-point reduction potentials of fmn and haem were determined by two complementary methods: potentiometric titration with substrate, l-lactate, in the presence of dye mediators with quantifica ... | 1986 | 3026360 |
| fermenting yeasts associated with softening and gas-pocket formation in olives. | fermenting, pectolytic yeasts were isolated from a massive commercial outbreak of softening and gas-pocket formation in olives that had been stored in acidified, low-salt brines in an attempt to reduce the problem of brine disposal. the suspected yeasts represented three different species: saccharomyces oleaginosus, s. kluyveri, and hansenula anomala var. anomala. all pectolytic cultures produced pectin esterase and polygalacturonase but no pectic acid trans-eliminase when grown in nutrient gluc ... | 1972 | 5017677 |
| pure l-lactate: cytochrome c oxidoreductase of the yeast hansenula anomala offers new prospect for the study of intramolecular multi-electron transfer. | | 1971 | 5148879 |
| [study of l (+)-lactate: cytochrome c oxidoreductase (cytochrome b 2 ) of the yeast hansenula anomala. i. preparation and characterization of the pure enzyme]. | | 1971 | 5150963 |
| utilization of bagassse pith for the production of fodder yeast by a new isolate of candida pelliculosa. | | 1969 | 5311992 |
| [effect of tryptophan analogues on l-tryptophan biosynthesis by hansenula anomala 315-21]. | | 1967 | 5618174 |
| hansenula anomala fungemia. | fungi of the genus hansenula have rarely been reported as pathogenic in humans. a case of catheter-related hansenula anomala fungemia in a patient with acute leukemia in remission is presented, and the clinical features of 11 additional patients infected with hansenula species are reviewed. the spectrum of disease with these organisms ranges from asymptomatic fungemia to life-threatening disseminated infection. predisposing factors appear to be immunosuppression, use of intravenous devices, and ... | 1988 | 3060946 |
| formation of ethyl acetate in hansenula anomala. | | 1968 | 5700790 |
| a clone coding for schizophyllum commune beta-glucosidase: homology with a yeast beta-glucosidase. | three identical clones coding for a partial sequence of the schizophyllum commune beta-glucosidase were isolated from a cdna library in lambda gt11, using polyclonal antibody to the enzyme. the identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-c digest with the sequence inferred from the cdna sequence. a comparison of the sequence with that reported for a beta-glucosidase from candida pelliculosa revealed a region in the latter with 43% identity in amino ... | 1986 | 3091028 |
| lytic action of beta-(1-3)-glucanase on yeast cells. | candida utilis, saccharomyces cerevisiae, s. fragilis, pichia polymorpha, and hansenula anomala yeast cells, harvested in the early logarithmic phase, were attacked with purified beta(1-3)-glucanase from micromonospora chalcea, which resulted in the liberation of protoplasts. the treated cells were observed under the electron microscope before the protoplasts were liberated. differences in the cell walls of the enzyme-treated and untreated cells were observed. the action of the glucanase was als ... | 1967 | 6027993 |
| pellicle formation and ester production by hansenula anomala. | | 1967 | 6054682 |
| [regulation of the biosynthesis and localization of nitrate deductase in hansenula anomala]. | | 1967 | 6059740 |
| yeast killer toxin-like anti-idiotypic antibodies. | anti-idiotypic antibodies (anti-ids) were raised in a rabbit against a murine monoclonal antibody (mab) neutralizing the yeast killer toxin produced by a strain of pichia (hansenula) anomala. in an immunodiffusion test, the anti-ids produced in the rabbit recognized the antigen-binding site of the mab used as the immunogen (kt4) but not that of another heterologous mab. the absence of any significant cross-reactivity among the anti-ids raised in a rabbit for a heterologous mab suggested that the ... | 1988 | 2451680 |
| flavin and heme structures in lactate:cytochrome c oxidoreductase: a resonance raman study. | resonance raman spectra of hansenula anomala l-lactate:cytochrome c oxidoreductase (or flavocytochrome b2), of its cytochrome b2 core, and of a bis(imidazole) iron-protoporphyrin complex were obtained at the soret preresonance from the oxidized and reduced forms. raman contributions from both the isoalloxazine ring of flavin mononucleotide (fmn) and the heme b2 were observed in the spectra of oxidized flavocytochrome b2. raman diagrams showing frequency differences of selected fmn modes between ... | 1989 | 2605171 |
| [killer protein, formed by the yeast hansenula anomala (hansen) h. et p. sydow]. | | 1989 | 2612351 |
| the microbial ecology of tape ketan fermentation. | the growth of fungi, yeasts and bacteria was followed during the fermentation of tape ketan. the tape was prepared using samples of indonesian ragi as inoculum. fermentation was characterized by the dominant growth of amylomyces rouxii and candida pelliculosa (10(5)-10(7) cfu/g), and a lesser contribution from saccharomyces cerevisiae. hansenula anomala grew to a limited extent during some fermentations. bacteria of the genera bacillus and acetobacter contributed also to the fermentation, produc ... | 1989 | 2641491 |
| sexual behavior and its pheromonal regulation in ascosporogenous yeasts. | we reviewed our investigations on sexual behaviors and interactions including sexual cell agglutination and pheromone action mainly in non-conventional yeasts, hansenula anomala, h. wingei, pichia amethionina, p. heedi, p. opuntiae, saccharomyces kluyveri, s. globsus, s. exiguus, saccharomycodes ludwigii. the techniques and genetic models including the cassette model and alpha 1-alpha 2 hypothesis which had been developed largely in s. cerevisiae were applicable to these yeasts in principle. the ... | 1989 | 2651648 |
| isolation of the flavodehydrogenase domain of hansenula anomala flavocytochrome b2 after mild proteolysis by an h. anomala proteinase. | the protomeric chain of hansenula anomala flavocytochrome b2 was previously shown to be built as the covalent association of two functional domains: an l-lactate dehydrogenase domain and a cytochrome c reductase domain, joined together by a proteolytically sensitive zone. this paper concerns the specific cleavage of this latter zone with a h. anomala proteinase(s) preparation and the purification of the resulting l-lactate dehydrogenase moiety of the molecule with at least 25% recovery, (i.e. on ... | 1989 | 2659353 |
| possible role of a 36 kda protein induced by respiratory inhibitors in cyanide-resistant respiration in hansenula anomala. | the antimycin a or cyanide-dependent appearance of a 36 kda protein in the particulate fraction was observed in l-[35s]methionine pulse-labeling experiments on cells of hansenula anomala, in which cyanide-resistant respiration was induced. the combined addition of cycloheximide or anaerobiosis, which block the induction of cyanide-resistant respiration, repressed the synthesis of this protein. these results suggest the involvement of the particulate 36 kda protein in cyanide-resistant respiratio ... | 1989 | 2768214 |
| nucleotide sequence of the hansenula anomala gene encoding flavocytochrome b2 (l-lactate:cytochrome c oxidoreductase). | | 1989 | 2813072 |
| nucleotide sequence of candida pelliculosa beta-glucosidase gene. | the nucleotide sequence of the dna fragment containing the beta-glucosidase gene of candida pelliculosa was determined. analysis of the sequence revealed three open reading frames which could encode 65,825, and 412 amino acid residues. the presence of the second frame was found to be sufficient for the expression of the beta-glucosidase gene in a heterologous host saccharomyces cerevisiae. putative protein encoded by this gene had hydrophobic amino acids, resembling a signal peptide, at its n-te ... | 1985 | 2995925 |
| fluorescence studies on the interaction between two cytochromes extracted from the yeast, hansenula anomala. | the binding of cytochrome c to the cytochrome b2 core, both extracted from the yeast, hansenula anomala, has been studied. cytochrome b2 core heme is extracted and replaced by the fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate (tns). a dissociation constant in the range of 85 microm is found for the tns-apoprotein complex with a stoichiometry of 1:1. the interaction between the two proteins is followed by monitoring changes in the tns fluorescence. we find the interaction between the c ... | 1985 | 2998284 |
| sensitivity of heat-stressed yeasts to essential oils of plants. | eight strains of yeasts (candida lipolytica, debaryomyces hansenii, hansenula anomala, kloeckera apiculata, lodderomyces elongisporus, rhodotorula rubra, saccharomyces cerevisiae, and torulopsis glabrata) were examined for changes in sensitivity to eight essential oils of plants (allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme) after being sublethally heat stressed. with the exception of garlic oil for all test yeasts, onion oil for s. cerevisiae, and oregano oil for r. rubr ... | 1984 | 6712207 |
| [joint cultivation of streptococcus lactis, lmu strain, and various species of yeasts]. | when s. lactis, strain lmu and yeast were plated out simultaneously on media containing different amounts of amine nitrogen and vitamins, the yeast did not stimulate the synthesis of nisin. plating out of s. lactis on media with grown cultures of the rostov baker's yeast, american baker's yeast, candida guilliermondii, candida clausenii, fabospora fragilis, fabospora macedoniensis, octosporomyces octosporus, saccharomyces cerevisiae, races "ya" and "m" and saccharomyces globosus resulted in inhi ... | 1985 | 3923918 |
| structure and chemical composition of prospheroplast envelopes of saccharomyces cerevisiae and hansenula anomala. | cells of saccharomyces cerevisiae and hansenula anomala were digested with snail enzyme under conditions yielding prospheroplasts. surrounding envelopes were isolated after lysis of prospheroplasts in distilled water. the envelope material was embedded and sectioned for electron microscopy, and thin, hollow structures still retaining the elongated form of the original cells were seen. the envelopes were of low electron density in sections stained with uranyl magnesium acetate and lead citrate, b ... | 1972 | 4552997 |
| 1h-nmr investigation of yeast cytochrome c. interaction with the corresponding specific reductase (l-lactate cytochrome). | 1h-nmr spectroscopy has been used to study the modifications of certain characteristic resonances of the hansenula anomala yeast cytochrome c on binding to its specific reductase (flavocytochrome b2) or to the isolated cytochrome domain obtained from the entire molecule. normal titration curves are observed for the resonances at 37.8 ppm assigned to heme c methyl 8 and at 19.4 ppm, line of cytochrome b2 spectrum. in contrast, the shifts near 3.2 and 3.4 ppm for trimethyl-lysine resonances of thi ... | 1987 | 3038091 |
| killer systems and pathogenic fungi. | our own studies on the yeast killer phenomenon have been concentrated on its application for the differentiation of opportunistic pathogenic yeast isolates within the same species and its use as an epidemiological marker in nosocomial infections caused by yeasts. our most recent investigations have led us to reevaluate the potential uses of this phenomenon, since it is now apparent that other microorganisms, unrelated to yeasts, are susceptible to the effects of these toxins. the yeast killer ph ... | 1988 | 3203721 |
| hansenula anomala: a new fungal pathogen. two case reports and a review of the literature. | fungal infections are characteristic of severely immunocompromised patients. noncandidal yeasts represent a growing proportion of such infections. risk factors for developing fungal infections include the use and abuse of central venous catheters. two patients with gynecologic malignant neoplasms became fungemic with hansenula anomala, a yeast of the ascomycetes class, after insertion of central venous catheters. frequent catheter manipulation and prolonged use favored the development of fungemi ... | 1988 | 3284503 |
| anti-candida albicans activity of pichia anomala as determined by a growth rate reduction assay. | killer toxin activity of pichia anomala wc65 appeared fungicidal for p. bimundalis wc38 and fungistatic for candida albicans rc1. inhibitory activity against sensitive c. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. the plot of toxin concentrations against growth rates was hyperbolic, as is characteristic of saturation kinetics. sensitivity of c. albicans to the toxin decreased with increased cell age. the measurement of growth r ... | 1988 | 3291760 |
| amino-acid sequence of the cytochrome-b5-like heme-binding domain from hansenula anomala flavocytochrome b2. | flavocytochrome b2 (l-lactate dehydrogenase) from baker's yeast is composed of two structural and functional domains. its first 100 residues constitute the heme-binding core, which is homologous to cytochrome b5 [b. guiard, o. groudinsky & f. lederer (1974) proc. natl acad. sci. usa 71, 2539-2543]. we report here the amino acid sequence of the heme-binding domain isolated by tryptic proteolysis of hansenula anomala flavocytochrome b2. the sequence was established by automated degradation of the ... | 1987 | 3319613 |
| biosensor for lactate determination in biological fluids. i. construction and properties of the biosensor. | the preparation of a biosensor for lactate determination is described. the biosensor is based on an immobilized suspension of the aerobic yeast hansenula anomala, containing flavocytochrome b2 in high activity. the conditions for yeast cultivation were optimized to gain a sufficiently high activity of this enzyme converting lactate in the cells. the properties of the biosensor are compared with those of a sensor based on immobilized enzyme flavocytochrome b2. the yeast lactate biosensor has a su ... | 1987 | 3549065 |
| the induction of cyanide-resistant respiration in hansenula anomala. | cyanide-resistant respiration was induced in the yeast, hansenula anomala in the presence of cyanide or antimycin a, which blocks the electron transport after ubiquinone. the de novo protein synthesis in cytosol and oxygen were deduced to be involved in this induction process. the period required for the induction varied during the growth stage, suggesting that involvement of additional physiological factor(s) in this induction process. the organism could multiply in the presence of antimycin a ... | 1987 | 3654588 |
| biosensor for lactate determination in biological fluids. 2. interference studies. | the selectivity of a yeast lactate biosensor with immobilized cells of aerobic yeast hansenula anomala was studied. reducing substances potentially present in blood plasma influenced both enzyme and yeast biosensors in the same way; the highest positive error was observed in the case of uric acid. with respect to the metabolic activity of the yeast cells the biosensor was absolutely specific for lactate during the first two weeks; later on the biosensor responded slightly to some other metabolit ... | 1987 | 3665087 |
| kinetics of electron transfer between two hansenula anomala flavocytochrome b2 derivatives and two simple copper proteins (azurin and stellacyanin). | two derivatives of hansenula anomala flavocytochrome b2 have been prepared, one deprived of the flavin prosthetic group (deflavocytochrome b2), and the other consisting of the heme-b-carrying globule (b2 core). the redox potential of the heme in the two derivatives is -5 (+/- 5) mv and -10 (+/- 5) mv respectively, fairly similar to the value of -20 (+/- 5) mv reported for the holoenzyme, indicating a minor effect of the flavin and of the flavodehydrogenase domain on heme potential. the kinetics ... | 1986 | 3780753 |
| interactions between killer yeasts and pathogenic fungi. | a total of 17 presumptive killer yeast strains were tested in vitro for growth inhibitory and killing activity against a range of fungal pathogens of agronomic, environmental and clinical significance. several yeasts were identified which displayed significant activity against important pathogenic fungi. for example, isolates of the opportunistic human pathogen, candida albicans, were generally very sensitive to williopsis mrakii killer yeast activity, whilst killer strains of saccharomyces cere ... | 1995 | 7758935 |
| infective endocarditis of a bicuspid aortic valve caused by hansenula anomala. | infective endocarditis due to hansenula anomala developed on a bicuspid aortic valve in a 40-year-old man. h. anomala, an ascomycetous yeast, may be a member of the normal flora of the throat and alimentary tract in humans but has not been previously known to be pathogenic in humans. a past history of intravenous drug use may have contributed to the development of disease in this patient. | 1987 | 3799679 |
| spontaneous dissociation of a cytochrome core and a biglobular flavoprotein after mild trypsinolysis of the bifunctional saccharomyces cerevisiae flavocytochrome b2. | saccharomyces cerevisiae flavocytochrome b2 is known as a bifunctional enzyme which behaves as the association of an fmn flavodehydrogenase with its specific acceptor, a b5-like cytochrome. mild trypsinolysis gives rise to three complementary fragments (n, x, beta'), both prosthetic groups being still bound. after such proteolysis the separation of a biglobular flavoprotein domain (carrying fmn) from a cytochrome domain (with the heme) is obtained by molecular sieving under non-denaturing condit ... | 1980 | 7007037 |
| exo-beta-glucanases in yeast. | 1. a number of yeast species were examined for the presence of beta-glucanases. extracts obtained by cell disruption of saccharomyces cerevisiae, fabospora fragilis and hansenula anomala hydrolysed laminarin and pustulan with the production of glucose. enzymic activities were also detected in the culture fluids of f. fragilis and h. anomala grown aerobically in buffered mineral medium with glucose as the carbon source. 2. f. fragilis and h. anomala possessed approximately sevenfold higher beta-( ... | 1968 | 5685856 |
| yeasts in ensiled high-moisture corn. | a total of 1,365 yeasts were selected from ensiled high-moisture corn at various stages in the ensiling process to determine the sequence and relative numbers of yeast species. the yeast species most frequently isolated from freshly harvested corn were candida parapsilosis and c. intermedia; these two species were isolated infrequently after the third week of storage. species of yeasts that predominate after the 12th day of storage were hansenula anomala (66% of the isolates studied) and c. krus ... | 1966 | 5914494 |
| influence of hansenula anomala yeast intake on the liver and kidney metabolism of the trout (salmo gairdneri). | the nutritional and metabolic effects of the use of hansenula anomala yeast as the only protein source were studied in the trout. food intake, weight and length increments significantly decreased in trout fed on yeast diet. hepatosomatic index did not suffer any change. soluble protein content in liver and kidney rose significantly. liver alkaline phosphatase activity increased significantly. liver lactate dehydrogenase (ldh) activity did not change in the experimental conditions. glutamic-oxala ... | 1983 | 6137315 |