| pichia norvegensis sp. nov. | a new yeast species pichia norvegensis leask et yarrow is described as the perfect state of candida norvegensis (dietrichson) van uden et farinha ex van uden et buckley. strains of this species were isolated on 3 occasions from human vaginas. this species differs from other pichia species that assimilate glucose but not galactose, sucrose, maltose, lactose, d-mannitol and d-glucitol by assimilating cellobiose. | 1976 | 1265575 |
| amino acid sequence of yeast hemoglobin. a two-domain structure. | the complete amino acid sequence of a hemoglobin from yeast (candida norvegensis) has been determined by peptide and cdna sequence analyses. the protein is composed of 387 amino acid residues and its amino terminus was blocked by an acetyl group. a computer search showed that the sequence of 155 n-terminal residues has 39% homology with that of vitreoscilla hemoglobin. on the other hand, the sequence of 230 c-terminal residues showed a small, but notable, degree of similarity with that of a meth ... | 1992 | 1404399 |
| candida norvegensis peritonitis and invasive disease in a patient on continuous ambulatory peritoneal dialysis. | we report a case of candida norvegensis invasive disease in an immunosuppressed renal transplant patient on continuous ambulatory peritoneal dialysis. multiple cultures of peritoneal fluid, blood, and tracheal suction done over a 2-week period were positive for this unusual isolate. despite treatment with amphotericin b and flucytosine the patient died. this is the first report of c. norvegensis fungemia documented by culture. | 1990 | 2380389 |
| in vitro susceptibilities of sucrose-negative candida tropicalis, candida lusitaniae, and candida norvegensis to amphotericin b, 5-fluorocytosine, miconazole, and ketoconazole. | the mics and minimal lethal concentrations of four antimycotics, amphotericin b, 5-fluorocytosine, miconazole nitrate, and ketoconazole, were determined for 25 yeast isolates representing species uncommonly implicated in candidiasis. a microdilution procedure was employed with complex and synthetic media. the isolates, in general, were susceptible to the same antimicrobial agents shown to be effective against candida albicans, but differences between some of the species in relative susceptibilit ... | 1984 | 6325491 |
| the unique structures of protozoan myoglobin and yeast hemoglobin: an evolutionary diversity. | a hemoglobin-like protein is found in some of the single-celled organisms, but its structure is quite different from that of mammalian myoglobin or hemoglobin. for instance, a protozoan myoglobin isolated from paramecium caudatum consists of 116 amino acid residues, so that this contracted form is nearly two thirds of sperm whale myoglobin. yeast hemoglobin from candida norvegensis, on the other hand, is composed of a single polypeptide chain with 387 amino acid residues, but of two distinct dom ... | 1995 | 7584595 |
| ability of rapid yeast plus system to identify 304 clinically significant yeasts within 5 hours. | the rapid yeast plus system (innovative diagnostic systems, norcross, ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. the ability of the rapid yeast plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. the rapid yeast plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. a further 12 strains (3.9%) were classif ... | 1996 | 8727877 |
| species-specific identification of candida krusei by hybridization with the ckf1,2 dna probe. | the species specificity of the candida krusei dna fingerprinting probe ckf1,2 has been investigated. a total of 149 pathogenic and nonpathogenic fungal and bacterial dnas were screened with ckf1,2. the probe was cold labeled with peroxidase, and its specificity was assessed by using southern blot, dot blot, and colony blot hybridization. its sensitivity was determined by dot blot hybridization. the ckf1,2 probe proved to be species specific. it hybridized with dna for the 112 c. krusei strains s ... | 1996 | 8784578 |
| invasive candida norvegensis infection in immunocompromised patients. | invasive infection with candida norvegensis has previously been reported only once. three new cases of invasive infection with c. norvegensis are described. one patient with acute myeloid leukaemia and neutropenic septicaemia had positive blood cultures with c. norvegensis, and 2 patients with aids and prolonged unexplained febrile cachexia had positive cultures of c. norvegensis from multiple sites, including pericardium, liver, kidneys lymph nodes and bone marrow, on autopsy. in severely immun ... | 1996 | 8863369 |
| isolation of candida norvegensis from clinical specimens: four case reports. | | 1996 | 8922831 |
| itraconazole for prophylaxis of systemic mycoses in neutropenic patients with haematological malignancies. | the efficacy of oral itraconazole 2 x 200 mg capsules daily for prevention of systemic mycoses was investigated in granulocytopenic patients with haematological malignancies. of 241 patients, 197 were evaluable for prophylactic efficacy, and 214 for adverse events. patients with similar characteristics receiving oral amphotericin b as antifungal prophylaxis, observed over 15 months before introduction of itraconazole, served as control group (n = 223). with itraconazole prophylaxis, 13 cases of ... | 1996 | 9023642 |
| analysis of population structure of cactophilic yeast from the genus pichia: p. cactophila and p. norvegensis. | dnas from 40 strains of pichia cactophila and pichia norvegensis, yeasts characteristic of cactus necroses, were compared using randomly amplified polymorphic dna (rapd) banding patterns and killer/sensitive phenotypes. both species belong to the same species complex within the genus. the levels of between-strain rapd variation were high in both species (higher in the automictic p. cactophila than in the heterothallic p. norvegensis), although there is little variation in physiological abilities ... | 1997 | 9057294 |
| species differentiation by internally transcribed spacer pcr and hhai digestion of fluconazole-resistant candida krusei, candida inconspicua, and candida norvegensis strains. | pcr amplification of the regions containing the internally transcribed spacers and 5.8s rrna gene of candida krusei, c. inconspicua, and c. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. hhai digestion of these fragments yielded species-specific bands. random amplification of polymorphic dna with primer r108 showed interspecific discriminatory band patterns. susceptibilities to fluconazole and amphotericin b were determined. | 1997 | 9157129 |
| candida norvegensis: a fluconazole-resistant species. | candida norvegensis has been an unusual cause of infections in humans. in norway this species was isolated from eight patients from 1990 to 1996 and was of probable pathogenic significance in four of them. all isolates were resistant to fluconazole. the same was true for two c. norvegensis isolates from before 1940, and it is therefore assumed that the fluconazole resistance is inherent. | 1997 | 9174202 |
| nosocomial breakthrough fungaemia during antifungal prophylaxis or empirical antifungal therapy in 41 cancer patients receiving antineoplastic chemotherapy: analysis of aetiology risk factors and outcome. | forty-one episodes of breakthrough fungaemia occurring over a 7.5 year period in the national and st elizabeth's cancer institutes in bratislava, slovakia, were analysed. five of them occurred during prophylaxis with fluconazole (one torulopsis glabrata, one hansenula anomala, two candida krusei and one candida parapsilosis), ten with itraconazole (three trichosporon pullulans, one trichosporon beigelii, one cryptococcus laurentii, three candida albicans and two t. glabrata), 11 during prophylax ... | 1998 | 9578164 |
| [evaluation of a new method for antifungal drugs susceptibility testing to yeasts]. | we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ... | 2000 | 10783576 |
| successful treatment with liposomal amphotericin b of an intraabdomianl abscess due to candida norvegensis associated with a gore-tex mesh infection. | there are few reports of severe infections caused by candida norvegensis. we here describe a case of c. norvegensis-associated intraabdominal abscess and gore-tex mesh-associated infection, successfully treated with liposomal amphotericin b and removal of the mesh. this is, to our knowledge, the first report of c. norvegensis causing this type of infection. | 2000 | 11055666 |
| fungal contamination of food in hematology units. | the prevalence of thermotolerant fungi on non-heat-sterilizable food was determined. aspergillus spp. were noted in 100% of pepper and regular tea samples, 12 to 66% of fruits, 27% of herbal teas, and 20% of freeze-dried soup samples. all soft cheese samples were contaminated by geotrichum and yeast (candida norvegensis) but candida albicans was never identified. | 2000 | 11060109 |
| characterization of two novel yeast strains used in mediated biosensors for wastewater. | after isolation from a pulp mill wastewater treatment facility, two yeast strains, designated spt1 and spt2, were characterized and used in the development of mediated biochemical oxygen demand (bod) biosensors for wastewater. 18s rrna gene sequence analysis revealed a one nucleotide difference between the sequence of spt1 and those of candida sojae and candida viswanthii. while spt2 had the highest overall homology to pichia norvegensis, at only 73.5%, it is clearly an ascomycete, based on blas ... | 2002 | 12109881 |
| yeast flavohemoglobin from candida norvegensis. its structural, spectral, and stability properties. | flavohemoglobin was isolated directly from the yeast candida norvegensis and studied on its structural, spectral, and stability properties. in candida flavohemoglobin, the 155 n-terminal residues make a heme-containing domain, while the remaining 234 c-terminal residues serve as a fad-containing reductase domain. a pair of his-95 and gln-63 was assigned to the proximal and distal residues, respectively. in purification procedure fad was partially dissociated on a butyl-toyopearl column, so that ... | 2002 | 12192008 |
| development and evaluation of the nuclisens basic kit nasba for the detection of rna from candida species frequently resistant to antifungal drugs. | we describe a nucleic acid sequence based amplification (nasba) protocol to detect 6 different candida species (candida krusei, candida glabrata, candida inconspicua, candida dubliniensis, candida norvegensis, candida lusitaniae) and compare it to a pcr assay. nasba showed a sensitivity of 1 colony forming unit and detected rna from all 6 candida species within 1 working day. all 5 patients with documented candidiasis showed identical results by both methods. this assay offers a sensitive, speci ... | 2003 | 12663165 |
| restriction enzyme analysis of ribosomal dna shows that candida inconspicua clinical isolates can be misidentified as candida norvegensis with traditional diagnostic procedures. | we identified 29 yeast isolates from 22 patients using the api id32c panel. twenty-eight of these isolates were candida norvegensis and one was c. inconspicua. although c. norvegensis is considered a pseudohypha-producing species, only one isolate produced pseudohyphae. restriction enzyme analysis of pcr-amplified ribosomal dna with four different enzymes proved that all isolates were c. inconspicua. | 2003 | 14605175 |
| surveillance of triazole susceptibility of colonizing yeasts in patients with haematological malignancies. | the species distribution and antifungal susceptibility of yeasts isolated from colonization cultures, and the contribution of previous fluconazole prophylaxis to the emergence of resistance, was prospectively studied in 87 neutropenic patients treated for haematological malignancies. minimal inhibition concentrations (mics) to fluconazole, itraconazole and voriconazole for 123 yeast isolates (65 candida albicans, 22 candida glabrata, 15 saccharomyces cerevisiae, 5 candida krusei, 5 candida norve ... | 2004 | 15764173 |
| reverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens. | we evaluated a combined panfungal pcr-reverse line blot (rlb) hybridization assay based on internal transcribed spacer 1 (its1) and its2 region polymorphisms to identify 159 candida, cryptococcus neoformans, and aspergillus isolates (22 species). its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. its sequence analysis was performed to resolve discrepant identifications or where no rlb result was obtained. species-specific its2- and its1-based probes ... | 2007 | 17634313 |
| evaluation of new colorimetric vitek 2 yeast identification card by use of different source media. | the new colorimetric vitek 2 yst card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. the vitek 2 yst card achieved satisfactory results for all yeast species tested, with the exception of candida guilliermondii, candida norvegensis, candida parapsilosis, candida rugosa, and candida tropicalis. after simple additional tests, 93.7% of all the strains tested were correctly identified. a significant influence of the isolation medium ... | 2008 | 18799700 |
| candida norvegensis fungaemia in a neutropenic patient with acute non-lymphoblastic leukaemia. | we report a case of fungaemia resulting from candida norvegensis in a patient with acute non-lymphoblastic leukaemia-m4 from turkey. candida norvegensis was isolated from two different peripheral blood samples that were taken at 2-day intervals. despite treatment with liposomal amphotericin b, the patient died of multi-organ system failure. | 2010 | 19496937 |
| biodiversity and phytase capacity of yeasts isolated from tanzanian togwa. | the focus of the present investigation was on the tanzanian fermented food togwa as a source for dietary iron and zinc, and the potential for mineral availability improvements using selected yeasts. to establish the content of target minerals and main inhibitor for intestinal uptake, iron and zinc as well as the mineral chelating phytic acid, (ip(6) or phytate) were determined in naturally fermented togwa. yeasts were isolated from sorghum, maize and cassava based togwa, and identified by sequen ... | 2010 | 19906458 |
| molecular identification of closely related candida species using two ribosomal intergenic spacer fingerprinting methods. | recent changes in the epidemiology of candidiasis highlighted an increase in non- candida albicans species emphasizing the need for reliable identification methods. molecular diagnostics in fungal infections may improve species characterization, particularly in cases of the closely related species in the candida complexes. we developed two pcr/restriction fragment length polymorphism assays, targeting either a part of the intergenic spacer 2 or the entire intergenic spacer (igs) of ribosomal dna ... | 2010 | 21227390 |
| diversity and killer activity of yeasts in malaysian fermented food samples. | the biodiversity and the killer activity of yeasts isolated from various types of fermented food in malaysia were investigated in this study. of 252 yeasts isolated from 48 fermented food samples in this study, 19 yeast species were identified based on sequence analysis of the its1-5.8s-its2 partial fragments of the yeasts. a total of 29 (11.5%) of the yeast isolates demonstrated killer activity to at least one candida species tested in this study; including 22 isolates of trichosporon ... | 2011 | 22041766 |
| Fungemia and renal fungus ball formation with Candida norvegensis in a child with acute lymphoblastic leukemia. | Invasive fungal infections cause significant morbidity and mortality in pediatric cancer patients. Candida species are the most frequently isolated pathogen. Candida species may cause bloodstream and deep-seated infection in neutropenic children with cancer. The gastrointestinal system, lung, liver and spleen are the most frequently involved organs. Isolated renal involvement presented as abscess formation has been reported rarely in children with cancer. Herein, we report a patient with acute l ... | 2011 | 21980850 |
| distribution of yeast species associated with oral lesions in hiv-infected patients in southwest uganda. | oropharyngeal candidiasis remains a significant clinical problem in hiv-infected and aids patients in regions of africa where anti-retroviral therapy isn't readily available. in this study we identified the yeast populations associated with oral lesions in hiv-infected patients in southwest uganda who were receiving treatment with nystatin and topical clotrimazole. samples were taken from 605 patients and 316 (52%) of these yielded yeast growth following incubation on sabouraud dextrose agar. sa ... | 2011 | 21905950 |
| candida inconspicua and candida norvegensis: new insights into identification in relation to sexual reproduction and genome organization. | candida inconspicua and candida (pichia) norvegensis are two emerging pathogenic species that exhibit reduced susceptibility to azole derivatives. conventional (biochemical) approaches do not readily differentiate between the two species. the first aim of this work was to analyze the performance of biochemical, proteomic (matrix-assisted laser desorption ionization-time of flight [maldi-tof]), and molecular approaches in the precise identification of these species. these results then led us to s ... | 2015 | 25762773 |
| genetic diversity of medically important and emerging candida species causing invasive infection. | genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis. | 2015 | 25887032 |
| unpasteurised commercial boza as a source of microbial diversity. | boza is a cereal-based fermented beverage widely consumed in many countries of the balkans. the aim of this study was to investigate the microbiota of three bulgarian boza samples through a combination of culture-dependent and -independent methods with the long-term objective of formulating a multi-strain starter culture specifically destined for the manufacture of new cereal-based drinks. the isolation campaign for lactic acid bacteria (lab) allowed the identification of lactobacillus parabuchn ... | 2015 | 25437059 |