| microorganisms and characteristics of laban. | laban had a titratable acidity of about 1.0%, a ph of 4.25, an ethanol content of 1.25%, and contained 4.2 mug acetaldehyde and 34 mug acetoin/ml. there was no diacetyl. five microorganisms, classified as streptococcus thermophilus, lactobacillus acidophilus, leuconostoc lactis, kluyveromyces fragilis, and saccharomyces cerevisiae, were responsible for the fermentation. streptococcus thermophilus and l. acidophilus were responsible for acid production with s. thermophilus producing acid more rap ... | 1976 | 2630 |
| histone-like protein fractions of kluyveromyces fragilis and their relation to the cell cycle. | protein fractions were obtained from kluyveromyces fragilis by ph titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized. the inclusion of a distilled water step resulted in a tenfold purification of the fraction obtained at ph 2.20 as compared with ph titration alone. in synchronous cultures induced with 2'-deoxyadenosine or prepared by selection this fraction displayed a stepwise accumulation, ... | 1976 | 9743 |
| characterisation and metabolic studies of saccharomyces cerevisiae and kluyveromyces fragilis by flow microcalorimetry. | the use of microcalorimetry in the routine identification of microorganisms is critically discussed and assessed. by use of flow microcalorimetric studies on saccharomyces cerevisiae and kluyveromyces fragilis the role of physical parameters and that of oxygen tension are discussed. the conclusion reached is that identification of microorganisms by microcalorimetry and subsequent discussion of metabolic events revealed by the thermogram, except under restrictive conditions, is inappropriate. how ... | 1979 | 45217 |
| defective blood mononuclear phagocyte function in patients with leprosy. | patients with lepromatous leprosy possess a defective lymphocyte function in vivo and in vitro that is less evident in the tuberculoid form. data concerning their macrophage ability to digest mycobacterium leprae are controversial. the purpose of this study was to determine whether monocytes from patients with either tuberculoid or lepromatous leprosy were altered in their enzyme systems, that is myeloperoxidase-dependent and myeloperoxidase-independent systems. the ability of adherent blood mon ... | 1979 | 122627 |
| [the effect of dissolved oxygen tension on the energy metabolism of yeasts]. | the specific atp generation rate in yeasts was examined on the glycolytic pathway and on the respiratory chain as a function of the dissolved oxygen tension of the culture medium. two different strains were used: saccharomyces cerevisiae sensitive to the glucose effect and kluyveromyces fragilis insensitive to the catabolite respression when growing on lactose. the oxidative atp generation rate followed by these two strains a michaelis menten kinetics against the dissolved oxygen concentration. ... | 1977 | 146544 |
| defective lung macrophages in pulmonary alveolar proteinosis. | lung macrophages obtained by segmental lavage from three patients with pulmonary alveolar proteinosis were studied in vitro. the macrophages exhibited morphologic abnormalities including excessive lipid accumulation and giant secondary lysosome formation. these cells survived poorly in tissue culture, showed impaired chemotactic activity, and had decreased adhesiveness to glass. they phagocytized normally but had substantially decreased capacity to kill ingested candida pseudotropicalis. evidenc ... | 1976 | 183578 |
| two-step separation of human peripheral blood monocytes on discontinuous density gradients of colloidal silica-polyvinylpyrrolidinone. | normal human peripheral blood monocytes were purified by a two-step separation. the first step, the standard ficoll--hypaque (f--h) buoyant density centrifugation, yielded mainly mononuclear cells, of which 24 +/- 9% were monocytes. isopycnic centrifugation on discontinuous gradients of colloidal silica polyvinylpyrrolidinone (cs-pvp) further separated these mononuclear cells. the density interface between 1.070 and 1.060 g/ml yielded 82 +/- 7% monocytes, 5 +/- 4% granulocytes and 13 +/- 8% lymp ... | 1977 | 201698 |
| the immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. | saccharomyces cerevisiae cells, kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. ethanol production from glucose solutions by an immobilized preparation of s. cerevisiae was deomonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days. immobilized k. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining pr ... | 1977 | 321046 |
| environmentally-induced changes in the neutral lipids and intracellular vesicles of saccharomyces cerevisiae and kluyveromyces fragilis. | saccharomyces cerevisiae, grown aerobically or anaerobically under conditions which induce a requirement for a sterol and an unsaturated fatty acid, synthesized approximately the same amounts of neutral lipid and intracellular low-density vesicles, although the neutral lipids in aerobically-grown cells contained more esterified sterol and less triacylglycerol than those in anaerobically-grown cells. kluyveromyces fragilis synthesized much less neutral lipid and a smaller quantity of low-density ... | 1977 | 334099 |
| evaluation of industrial yeasts for pathogenicity. | eleven yeasts representative of species of industrial interest were compared with candida albicans for their potential pathogenicity for untreated and cortisone-treated mice. only c. tropicalis produced a progressive infection similar to that produced by c. albicans. candida lipolytica, torulopsis spp., and hansenula polymorpha were not recovered from mice 6 days after inoculation. kluyveromyces fragilis, c. pseudotropicalis, c. utilis, c. guilliermondii and c. maltosa were recovered from mice b ... | 1979 | 441902 |
| studies on the growth of kluyveromyces fragilis in whey for the production of yeast protein. | | 1978 | 583484 |
| flow microcalorimetric investigation of yeast growth in a complex medium. | the growth of kluyveromyces fragilis in a complex medium under anaerobic and aerobic growth conditions has been conducted using a flow microcalorimeter. growth under defined conditions is characterized by an initial exponential rise in heat output rate to a maximum, followed by a decline to a baseline deflection. inadequate oxygenation can result in a more structured thermogram. the results are used to illustrate the deficiencies of some calorimetric incubations, and observations reported by oth ... | 1978 | 753949 |
| ultrastructural changes, caused by the new antibiotic mucidin in candida pseudotropicalis. | | 1976 | 947119 |
| a study on transformation in a mutant of candida pseudotropicalis. | this paper reports on the possible influence of various factors on the frequency of transformation in candida pseudtropicalis. the concentration of dna used varied between 25 mug and 100 mug/ml. the maximum percentage transformation 0-16% occured at 75 mug/ml. | 1976 | 959946 |
| accumulation of ergosterol during the cell cycle of the budding yeast kluyveromyces fragilis. | | 1976 | 983132 |
| [alcohol dehydrogenase (adh) in yeast cells. i. cytoplasmic, mitochondrial and nuclear adh in saccharomyces carlsbergensis and kluyveromyces fragilis]. | when grown in a medium containing lactat, saccharomyces carlsbergensis produces 5 times more ground-plasmatic adh than kluyveromyces fragilis. upon gelectrophoresis, k. fragilis exhibits 7 bands while s. carlsbergensis shows only one. in a polyacrylamid gradient, the adh's of both strains yield one band, the position of which corresponds to a molecular weight of about 160 000 d. in cell homogenates and mitochondrial fractions of s. carlsbergensis treated with ultrasound, an adh is detected which ... | 1976 | 983135 |
| 2'-deoxyadenosine and a23187 as agents for inducing synchrony in the budding yeast, kluyveromyces fragilis. | the effects of induction synchrony by 2'-deoxyadenosine and the ionophore a23187 were compared with selection synchrony methods in kluyveromyces fragilis. 2'-deoxyadenosine synchronizes nuclear division and this is followed by synchronous cell division, but dna synthesis is not synchronized. the ionophore a23187 also induces synchronous nuclear division and synchronous cell division, but likewise does not synchronize dna synthesis. | 1975 | 1100777 |
| the effects of various growth temperatures on nuclear division, dna and rna in the budding yeast, kluyveromyces fragilis. | | 1975 | 1176961 |
| selection of strain, growth conditions, and extraction procedures for optimum production of lactase from kluyveromyces fragilis. | forty-one strains of kluyveromyces fragilis (jörgensen) van der walt 1909 varied 60-fold in ability to produce lactase (beta-galactosidase). the four best strains were ucd no. 55-31 (northern regional research center nrrl y-1196), ucd no. c21(-), ucd no. 72-297(-), and ucd no. 55-61 (nrrl y-1109). biosynthesis of lactase during the growth of k. fragilis strain ucd no. 55-61 was followed on both lactose and sweet whey media. maximum enzyme yield was obtained at the beginning of the stationary pha ... | 1975 | 1194465 |
| synergistic action of amphotericin b and rifampin against candida species. | amphotericin b and rifampin act synergistically against certain yeasts in vitro. whether this synergism is a general phenomenon or whether the effect has strict species and strain requirements was studied. included in a survey of the genus candida were eight human isolates of candida albicans and one strain each of candida krusei, candida tropicalis, candida pseudotropicalis, candida parapsilosis, candida guilliermodnii, and candida stellatoidea. cultures in both control and drug-containing liqu ... | 1976 | 1245767 |
| [the role of candida kefyr (c. pseudotropicalis) in reproductive tract infections]. | frequency of appearance of c. kefyr strains, their diagnosis, susceptibility to drugs and importance in pathogenesis of reproductive tract mycoses were investigated. the investigated material consisted of 2717 strains, and there were 67 strains of c. kefyr isolated from vaginal mucous membrane. c. kefyr strains constituted 2.5% of total number of 2717 isolated strains and 8.2% within fungi other than c. albicans species. vaginal mycosis caused by c. kefyr occurs in presence of lactobacillus sp. ... | 1992 | 1297040 |
| photodynamic treatment of yeast cells with the dye toluidine blue: all-or-none loss of plasma membrane barrier properties. | photodynamic treatment of kluyveromyces marxianus with the sensitizer toluidine blue leads to the loss of colony forming capacity. in this paper, the influence of this treatment on the barrier properties of the plasma membrane has been studied. photodynamic treatment with the dye toluidine blue resulted in efflux of potassium ions and e260-absorbing material. moreover, cells became stainable with erythrosine. it is concluded that the permeability change induced by photodynamic treatment proceeds ... | 1992 | 1322699 |
| trichothecene synergism, additivity, and antagonism: the significance of the maximally quiescent ratio. | the interactive effect of the combinations of trichothecene mycotoxins often found in fungus infected plants, contaminated grain, and other biological systems is poorly understood. growth inhibition of the yeast kluyveromyces marxianus was used to measure the effects of ht-2 toxin, roridin a, and t-2 toxin as individual toxins or as binary mixtures. a value, the combination index, was derived which indicates the interactive effects of a binary mixture of toxins. the interaction is affected by th ... | 1992 | 1344899 |
| a protein homologous to glyceraldehyde-3-phosphate dehydrogenase is induced in the cell wall of a flocculent kluyveromyces marxianus. | a protein with an apparent molecular weight of 37,000 (p37) is present in very large amounts in the cell wall of kluyveromyces marxianus, after the induction of flocculation of the yeast. this protein was isolated by preparative gel electrophoresis and its purity checked by sds-page and reverse-phase hplc. sds-page, endoglycosidase-h treatment and peptide sequencing indicated that p37 is a glycoprotein with a high identity to cytosolic glyceraldehyde-3-phosphate dehydrogenase from saccharomyces ... | 1992 | 1390912 |
| microenvironment at the substrate binding subsite of the active site of udpglucose 4-epimerase from kluyveromyces fragilis using a fluorescent analog of ump. | a chromophorics and fluorescent analog of uridine 5'-monophosphate (ump), a known competitive inhibitor of udpglucose 4-epimerase was synthesised. this analog, namely 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) uridine 5'-monophosphate, was found to be a powerful reversible inhibitor of udpglucose 4-epimerase indicating its interaction with the substrate binding site of the enzyme. the extreme sensitivity of the fluorescence emission spectrum of this analog to solvent polarity makes it an excel ... | 1992 | 1398715 |
| synthesis of some hydroxamic acid derivatives of benzimidazole and their antibacterial and antifungal activities. | a number of 1h-benzimidazole-2-propanoic acid derivatives have been synthesized by phillips method, and their antibacterial and antifungal activities have been tested. the chemical structures of the synthesized compounds were elucidated by spectroscopic (ir, nmr, mass) and elementary analysis. investigation of antimicrobial activity of the compounds was done by agar dilution technique using bacteria (staphylococcus aureus atcc 25923, escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853 ... | 1992 | 1418079 |
| growth of fermentative and non-fermentative yeasts in natural yoghurt, stored in polystyrene cartons. | permeation of oxygen through polystyrene packaging is a factor in the growth of yeasts in natural yoghurt. diffusion of oxygen through the packaging material can permit the growth of non-fermentative yeasts in yoghurt stored at refrigeration temperatures. yarrowia lipolytica, a non-fermentative yeasts which does not utilize lactose was isolated from yoghurt. the growth in natural yoghurt of yarrowia lipolytica and the lactose-fermenting yeast kluyveromyces marxianus was investigated. both yeasts ... | 1992 | 1419544 |
| structural and putative regulatory sequences of kluyveromyces ribosomal protein genes. | the transcription of the majority of the ribosomal protein (rp) genes of saccharomyces cerevisiae is activated by cis-acting elements, designated rpg boxes, which specifically bind the multifunctional protein rap1 in vitro. to investigate to what extent this global system of transcription regulation has been conserved, we have isolated a number of rp genes of the related yeast species kluyveromyces lactis and kluyveromyces marxianus, whose counterparts in saccharomyces are controlled by rap1. th ... | 1992 | 1481569 |
| structure and expression of the abf1-regulated ribosomal protein s33 gene in kluyveromyces. | the abundant multifunctional protein abf1 of saccharomyces cerevisiae binds to the upstream region of several genes, including some ribosomal protein genes like the one encoding protein s33. deletion of the abf1-binding sequence lowers the transcription of these genes three- to more than ten-fold. we have isolated the s33 genes of two related yeast species, kluyveromyces lactis and kluyveromyces marxianus. comparison of the nucleotide sequences of these s33 genes with their counterpart from s. c ... | 1992 | 1481571 |
| effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. | addition of benzoate to the medium reservoir of glucose-limited chemostat cultures of saccharomyces cerevisiae cbs 8066 growing at a dilution rate (d) of 0.10 h-1 resulted in a decrease in the biomass yield, and an increase in the specific oxygen uptake rate (qo2) from 2.5 to as high as 19.5 mmol g-1 h-1. above a critical concentration, the presence of benzoate led to alcoholic fermentation and a reduction in qo2 to 13 mmol g-1 h-1. the stimulatory effect of benzoate on respiration was dependent ... | 1992 | 1523884 |
| an essential histidine residue for the activity of udpglucose 4-epimerase from kluyveromyces fragilis. | udpglucose 4-epimerase from kluyveromyces fragilis was completely inactivated by diethylpyrocarbonate following pseudo-first order reaction kinetics. the ph profile of diethylpyrocarbonate inhibition and reversal of inhibition by hydroxylamine suggested specific modification of histidyl residues. statistical analysis of the residual enzyme activity and the extent of modification indicated modification of 1 essential histidine residue to be responsible for loss in catalytic activity of yeast epim ... | 1992 | 1601847 |
| distinct functional roles of two active site thiols in udpglucose 4-epimerase from kluyveromyces fragilis. | udpglucose 4-epimerase from kluyveromyces fragilis was earlier shown to have two conformationally vicinal thiols at the active site. upon treatment with diamide, these thiols form a disulfide linkage across the subunits that results in coordinated loss of catalytic activity and coenzyme fluorescence (ray, m., and bhaduri, a. (1980) j. biol. chem. 255, 10777-10786). employing a number of thiol-specific reagents, we now suggest discriminatory and nonidentical roles for these two thiols. kinetic an ... | 1992 | 1601848 |
| effect of aeration rate on the alcoholic fermentation of whey by kluyveromyces fragilis. | in this paper, the influence of aeration rate on the alcoholic batch fermentation of whey by kluyveromyces fragilis nrrl y-2415 was investigated. assays in 1.5-l fermentor using concentrated whey permeate containing 100 g/l of lactose were carried out at different oxygen supply rate (klac*) from 0 to 82 mmol/lh. optimum response was obtained at 14 mmol/lh: ethanol production rate reached was 3.4 g/lh yielding 0.46 g of product per gram of initial lactose. an increase of klac* from 0 to 14 mmol/l ... | 1992 | 1605917 |
| single cell protein production from beet pulp by mixed culture. | different mixed cultures of trichoderma reesei and a yeast were cultivated on beet pulp (bp)-containing medium. t. reesei and kluyveromyces marxianus offered a combination that gave high scp yields (51%) and efficiently converted bp into proteins (39.4%). the yeast extract in the basal medium could be substituted and the bp level multiplicated from 2 to 4% upon using mixed culture of t. reesei and k. marxianus. under these conditions the protein yields reached to a maximum value of 54% and highe ... | 1992 | 1605920 |
| proton-motive force-driven d-galactose transport in plasma membrane vesicles from the yeast kluyveromyces marxianus. | galactose transport was studied in membrane vesicles, prepared by fusion of plasma membranes from the yeast kluyveromyces marxianus with proteoliposomes containing beef heart cytochrome c oxidase as a proton-motive force-generating system. sugar transport studies performed under nonenergized conditions revealed that, even at high protein to phospholipid ratios, not all vesicles contained a d-galactose-specific transporter. the amount of vesicles containing an active carrier proved to be proporti ... | 1991 | 1648083 |
| temperature induced changes in microsomal (na+,k+)-atpase and lipid composition of candida kefyr. | growth temperature affected both the membrane lipid composition and microsoma (na+, k+)-atpase activity of candida kefyr. higher growth, temperature (37 degrees c) increased the amount of total lipids, phospholipids and free sterol. ratios of phosphatidylcholine to phosphatidylethanolamine as well saturated to unsaturated fatty acids increased with a rise in growth temperature. km of the atpase isolated from the yeast grown at 27 degrees c was minimum, suggesting that the membranes of c. kefyr g ... | 1991 | 1666728 |
| transport of lactic acid in kluyveromyces marxianus: evidence for a monocarboxylate uniport. | lactic acid-grown cells of a strain of kluyveromyces marxianus transported d- and l-lactic acid by a saturable mechanism that was partially inducible and subject to glucose repression, with the following kinetic parameters at ph 5.4: vmax = 1.00 (+/- 0.13) mmol h-1 per g dry weight and ks = 0.42 (+/- 0.08) mm. lactic acid transport was competitively inhibited by pyruvic, glycolic, acetic and bromoacetic acids. the latter, a non-metabolizable analogue, was transiently accumulated, the extent depe ... | 1991 | 1789000 |
| cloning and disruption of the leu2 gene of kluyveromyces marxianus cbs 6556. | the leu2 gene, coding for beta-isopropylmalate dehydrogenase, of the yeast kluyveromyces marxianus was isolated and sequenced. an open reading frame, coding for a protein with a molecular weight of 38 kda was found. comparison of the deduced amino acid sequence of the leu2 gene with the corresponding enzymes of three other yeasts and two thermophilic bacteria, revealed extensive sequence similarities. the cloned gene could complement a leub mutation of escherichia coli and a leu2 mutation of sac ... | 1991 | 1803820 |
| [biological value of the unicellular protein of kluyveromyces marxianus var. lactis]. | the ever increasing problem of environmental pollution and protein scarcity led us to begin producing protein biomass from cheese whey, having selected for this aim, kluyveromyces marxianus va. lactis strain, which represents the right relationship between its protein content (53.3% d.w.) and that of the rna (4.63% d.w.). on the other hand, the distribution of its essential amino acids is balanced, although it shows a deficiency in methionine, its value being 1.5 g/16 gn. the purpose of this wor ... | 1991 | 1822070 |
| ciclopirox nail lacquer 8%: in vivo penetration into and through nails and in vitro effect on pig skin. | this report presents original methods to assess the bioavailability of an antifungal drug from a varnish preparation in finger nails. for the studies with human volunteers a ciclopirox 8% nail lacquer was used to determine its efficacy in the treatment of onychomycoses. in vivo studies were performed on the fingernails of healthy volunteers by determining the total amount of ciclopirox penetrated per milligram of nail and the partition of the drug in the plate of the nails (technically divided i ... | 1991 | 1831626 |
| cloning and sequencing of the inulinase gene of kluyveromyces marxianus var. marxianus atcc 12424. | cell wall inulinase (ec 3.2.1.7) was purified from kluyveromyces marxianus var. marxianus (formerly k. fragilis) and its n-terminal 33-amino acid sequence was established. pcr amplification of cdna with 2 sets of degenerate primers yielded a genomic probe which was then used to screen a genomic library established in the yep351 yeast shuttle vector. one of the selected recombinant plasmids allowed an invertase-negative saccharomyces cerevisiae mutant to grow on inulin. it was shown to contain an ... | 1991 | 1840529 |
| t-2 toxin degradation by micromycetes. | the biodegradation of t-2 toxin was studied by strains of micromycetes which were isolated from the environment. the 26 tested strains were divided into three groups. group contains strains which degraded t-2 toxin very fast. this toxin could not be chromatographically determined in the medium even after 48 hours of incubation and the antifungal activity of residua against kluyveromyces fragilis ccy-51-1-2 was low or zero. there were strains of alternaria sp., ulocladium sp., aspergillus candidu ... | 1991 | 1880407 |
| synthesis and antifungal activity of some new benzimidazole derivatives. | synthesis and antifungal evaluation of 5-ethoxycarbonyl-2-(substituted-benzyl or phenoxymethyl)benzimidazoles are reported. structures of the compounds were elucidated with ir-, 1h-nmr-, 13c-nmr-, mass-spectra and elemental analysis. preliminary results show that none of the synthesized benzimidazole derivatives has antifungal activity at the concentration of 100 micrograms/ml against candida parapsilosis, candida stellatoidea, and candida pseudotropicalis. | 1991 | 1888265 |
| antimicrobial activity of microgard against food spoilage and pathogenic microorganisms. | microgard, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for gram-negative bacteria such as pseudomonas, salmonella, and yersinia when 1% concentration was incorporated into agar media. gram-positive bacillus cereus, staphylococcus aureus, and listeria monocytogenes were insensitive to microgard. kluyveromyces marxianus, an unidentified black yeast, and penicillium expansum were partially suppressed, whereas aspergillus niger and a y ... | 1991 | 1906486 |
| evolutionary relationships among pathogenic candida species and relatives. | small subunit rrna sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus candida (including torulopsis [candida] glabrata and yarrowia [candida] lipolytica) and for hansenula polymorpha. phylogenetic analyses of these sequences and those of saccharomyces cerevisiae, kluyveromyces marxianus var. lactis, and aspergillus fumigatus indicate that candida albicans, c. tropicalis, c. parapsilosis, and c. viswanathii form a subgroup within the genus ... | 1991 | 2007550 |
| effects of growth temperature on toxicity of t-2 toxin and roridin a to yeast. | in yeasts, growth temperature is known to affect the membrane phospholipid content. the effect of temperature on the growth inhibition of kluyveromyces marxianus and saccharomyces cerevisiae by the trichothecene mycotoxins, t-2 toxin and roridin a, was investigated. examination of ec50 values for t-2 toxin and roridin a showed that these toxins were least inhibitory to both yeasts at 30 and 25 degrees c, respectively. increasing or decreasing growth temperature from these temperatures gradually ... | 1991 | 2043279 |
| chemical composition of a mixture of single-cell protein obtained from kluyveromyces fragilis and whey proteins. | a mixture of kluyveromyces fragilis biomass and coagulated whey proteins was obtained by fermentation of whole whey. this product had a chemical composition similar to that of washed products reported in the literature with high-crude protein and low-ash contents. the product had a high content of sulphur-containing amino acids and tryptophan, which are usually limiting in yeast biomass. lysine content was inexplicably lower than the expected value, being the limiting amino acid in this case. th ... | 1990 | 2133184 |
| structure and properties of the extracellular inulinase of kluyveromyces marxianus cbs 6556. | in the yeast kluyveromyces marxianus two forms of inulinase were present, namely, an inulinase secreted into the culture fluid and an inulinase retained in the cell wall. both forms were purified and analyzed by denaturing and nondenaturing polyacrylamide gel electrophoresis. with the use of endo-beta-n-acetyl-glucosaminidase h, it was established that the enzyme retained in the cell wall and the enzyme secreted into the culture fluid have similar subunits consisting of a 64-kda polypeptide with ... | 1990 | 2135869 |
| [chemical composition of the cellular biomass of yeasts]. | this work is aimed at analyzing yeast strains, possibly used in animal feeding, obtained by batch cultivation from cheese whey as main carbohydrated substrate. for that purpose 10 yeast strains selected for its biomass production capacity were chemically analyzed. from the results, it can be observed that the chemical composition of the strains is quiet variable, showing in all cases high protein content, good solubility and enzymatic digestibility. in all of them, the rna content is low, being ... | 1990 | 2136518 |
| continuous-culture study of the regulation of glucose and fructose transport in kluyveromyces marxianus cbs 6556. | regulation of transport of d-glucose and d-fructose was studied in kluyveromyces marxianus grown in continuous culture. both substrates could be transported by at least two different transport systems, low-affinity transport and high-affinity proton-sugar symport. the low-affinity transporter, specific for both glucose and fructose, was constitutively present and was apparently not regulated by carbon catabolite repression. regulation of the activity of the glucose- and fructose-specific proton ... | 1990 | 2160928 |
| effect of polymyxin b nonapeptide and polymyxin b sulphate on trichothecene mycotoxin sensitivity of yeasts using a conductimetric instrument. | the addition of polymyxin b sulphate (pbs), or an inactive by-product, polymyxin b nonapeptide (pbn) to a yeast bioassay system, increased its sensitivity to various toxic agents. the nil effect level (nel) of t-2 toxin was reduced from 0.1 to 0.01 microgram/ml for kluyveromyces fragilis gk 1005 in the presence of these agents when using a malthus at 192 conductimetric instrument. other synergistic agents (dmso, ethanol, cetyl trimethyl ammonium bromide and triton x-100) gave poor results in the ... | 1990 | 2168726 |
| human immunodeficiency virus causes mononuclear phagocyte dysfunction. | there is compelling clinical evidence for dysfunction of the mononuclear phagocyte system in patients with aids, which is believed due in part to loss of t-cell cooperativity. the direct consequences of human immunodeficiency virus infection on macrophage function are unknown. to address this question we infected normal human macrophages in vitro with a monocytotropic strain of human immunodeficiency virus and performed assays to quantify their extra- and intracellular killing ability. human imm ... | 1990 | 2187195 |
| extraction, purification and characterization of adh1 from the budding yeast kluyveromyces marxianus. | the enzyme adh1 has been extracted and purified from the budding yeast kluyveromyces marxianus, and its enzymatic activity has been compared, with the adh1 extracted and purified in the same way from the well known yeast saccharomyces cerevisiae. k. marxianus adh1 has an optimal temperature higher than the s. cerevisiae enzyme (45-50 degrees vs 35 degrees c), a better stability to ph variations in the oxidative reaction (ph optimum 7.5), a lower michaelis constant for acetaldehyde, and a good ca ... | 1990 | 2193901 |
| discriminant analysis of cellular fatty acids of candida species, torulopsis glabrata, and cryptococcus neoformans determined by gas-liquid chromatography. | we used discriminant analysis of cellular fatty acid compositions determined by gas-liquid chromatography to differentiate yeastlike fungi (a total of 190 strains; including 37 candida albicans strains, 21 candida krusei strains, 13 candida guilliermondii strains, 37 candida tropicalis strains, 10 candida pseudotropicalis strains, 24 candida parapsilosis strains, 32 torulopsis glabrata strains, and 16 cryptococcus neoformans strains). previous results with a standard strain of c. albicans indica ... | 1990 | 2199515 |
| cloning, sequencing and analysis of expression of a butyrivibrio fibrisolvens gene encoding a beta-glucosidase. | the cloning, expression and nucleotide sequence of a 3.74 kb dna segment on pls215 containing a beta-glucosidase gene (bgla) from butyrivibrio fibrisolvens h17c was investigated. the b. fibrisolvens bgla open reading frame (orf) of 2490 bp encoded a beta-glucosidase of 830 amino acid residues with a calculated mr of 91,800. in escherichia coli c600(pls215) cells the beta-glucosidase was localized in the cytoplasm and these cells produced an additional protein with an apparent mr of approximately ... | 1990 | 2262790 |
| localization of inulinase and invertase in kluyveromyces species. | in vivo hydrolysis of inulin and sucrose was examined in selected yeasts of the genus kluyveromyces. cells, grown in sucrose-limited chemostat cultures, were subjected to treatments for the removal of inulinase, the enzyme responsible for the hydrolysis of both inulin and sucrose. the effects of these treatments were studied by measurement of inulin-dependent and sucrose-dependent oxygen consumption by cell suspensions. in kluyveromyces marxianus var. marxianus, inulinase was partially secreted ... | 1990 | 2268150 |
| the yeasts of cheese brines. | a total of 365 yeasts were isolated from the brines of soft, semihard and hard cheeses from different manufacturers. identification was based on 131 characteristics, primarily employing a method with microtitration plates. most brines exhibited a characteristic yeast flora. the predominant strains proved to be mainly debaryomyces hansenii and candida versatilis. in a few brines trichosporon beigelii, c. rugosa, c. intermedia, kluyveromyces marxianus, saccharomyces sp. and c. tenuis/polymorpha we ... | 1990 | 2282287 |
| modes of lactose uptake in the yeast species kluyveromyces marxianus. | twelve lactose-assimilating strains of the yeast species kluyveromyces marxianus and its varieties marxianus, lactis and bulgaricus were studied with respect to transport mechanisms for lactose, glucose and galactose, fermentation of these sugars and the occurrence of extracellular lactose hydrolysis. the strains fell into three groups. group i (two strains): fermentation of lactose, glucose and galactose, extracellular lactose hydrolysis, apparent facilitated diffusion of glucose and galactose; ... | 1990 | 2321931 |
| occurrence of the general control of amino acid biosynthesis in yeasts. | the response of three amino acid biosynthetic enzymes, threonine dehydratase, tyrosine aminotransferase and saccharopine dehydrogenase, to conditions of histidine, tryptophan or lysine limitation was investigated in 15 yeast species. the activities of all these enzymes increased about two- to fourfold as a result of action of the general control of amino acid biosynthesis in brettanomyces anomalus, candida maltosa, hansenula polymorpha, rhodosporidium toruloides, saccharomyces cerevisiae and yar ... | 1990 | 2338623 |
| interaction of hexadecyltrimethylammonium bromide with yeast cells. | the interaction of hexadecyltrimethylammonium bromide (ctab) with two yeast cells, kluyveromyces fragilis and saccharomyces cerevisiae, has been studied. strong binding of ctab to the cell was inferred from 1h and 13c nmr studies, the probable site of binding being the cell-surface. 13c and 31p nmr studies have indicated facilitation of free passage of molecules from outside to inside the cell and vice versa on treatment with ctab. 31p nmr studies showed that intracellular ph (phi) was affected ... | 1990 | 2354849 |
| lectin mediated agglutination of candida kefyr cells and their spheroplasts grown in sterol enriched growth medium and its correlation with lipid composition. | supplementation of the growth medium with erosterol, cholesterol and lanosterol enriched the candida kefyr cells, presumably cell membranes with sterols. sterol enriched c. kefyr cells showed a decrease in percentage of pha and con-a mediated agglutination. sterol supplementation also increased the sterol: phospholipid ratio and in such cells unsaturated fatty acids predominated over saturated ones. the overall effect of these changes resulted in rigidifying the cell membranes as indicated by sh ... | 1988 | 2467528 |
| lipid composition and lectin-mediated agglutination of candida kefyr cells grown in media enriched with choline or its analogues. | candida kefyr cells and their spheroplasts grown in media enriched with choline, n,n'-dimethylethanolamine (dmea) or ethanolamine (ea) showed decreased concanavalin a (con a) and ph to hemagglutinin (pha) mediated agglutination while supplementation with n-monomethylethanolamine (mmea) increased pha-mediated agglutination. in all cases, the amount of phospholipid was increased and, consequently, a decrease in the free sterol; phospholipid ratio was observed except in the case of mmea where this ... | 1989 | 2486007 |
| nucleotide sequence of the clostridium thermocellum bgib gene encoding thermostable beta-glucosidase b: homology to fungal beta-glucosidases. | the nucleotide sequence of the bglb gene, coding for the thermostable beta-glucosidase b of clostridium thermocellum was determined. the coding region of 2265 bp was identified by comparison with the n-terminal amino acid sequence of beta-glucosidase b purified from escherichia coli. the derived amino acid sequence corresponding to a polypeptide of mr 84,100 was confirmed by sequencing of the c-terminal peptide generated by cleavage with cyanogen bromide. the protein bears no resemblance to othe ... | 1989 | 2505054 |
| [the search for microorganisms sensitive to deoxynivalenol, zearalenone and aflatoxin b1]. | sensitivity to t-2 toxin, deoxynivalenol (dov), zearalenon (zl) and aflatoxin (at) b1 was comparatively studied in yeast strains saccharomyces fragilis 25 d, candida pseudotropicalis 44 nk and saccharomyces lactis bkmy-459, sensitive to t-2 toxin, and strain bacillus megaterium bkmb-44, sensitive to toxic metabolites of aspergillus species of fungi. minimum amounts of t-2 toxin inducing on silufol plates sites of the yeast strain growth suppression, comprised 20-50 ng, maximum sensitivity was re ... | 1989 | 2534250 |
| [in vitro activity of fluconazole, a novel bistriazole antifungal agent]. | fluconazole is a novel triazole antifungal agent with excellent activities against a broad range of medically important fungi. the in vitro antifungal activities of fluconazole especially against candida albicans were examined and the results summarized as follows: 1. fluconazole was proved to exhibit the highest antifungal activity in synthetic amino-acid medium, fungal (saamf), well buffered in a physiologically neutral range. 2. in a exposed time-killing test performed using saamf (ph 7.4), t ... | 1989 | 2540356 |
| molecular probes for the detection of kluyveromyces marxianus chromosomal dna in electrophoretic karyotypes of intergeneric protoplast fusion products. | random genomic dna fragments from kluyveromyces marxianus were cloned in order to identify chromosomal bands in pulsed field electrophoresis patterns of intergneric hybrid strains which were obtained by protoplast fusion. molecular hybridization data indicated that the k. marxianus parental strain might be triploid, and it showed strong chromosome length polymorphism. we analyzed the karyotype of two saccharomyces cerevisiae/k. marxianus hybrid strains (st. 1.st.46) with our dna probes and with ... | 1989 | 2573327 |
| ammonium assimilation by candida albicans and other yeasts: evidence for activity of glutamate synthase. | activities and properties of the ammonium assimilation enzymes nadp+-dependent glutamate dehydrogenase (gdh), glutamate synthase (gogat) and glutamine synthetase (gs) were determined in batch and continuous cultures of candida albicans. nadp+-dependent gdh activity showed allosteric kinetics, with an s0.5 for 2-oxoglutarate of 7.5 mm and an apparent km for ammonium of 5.0 mm. gogat activity was affected by the buffer used for extraction and assay, but in phosphate buffer, kinetics were hyperboli ... | 1989 | 2575653 |
| synthesis and biological activity of 6-acyl-3-substituted-2(3h)-benzoxazolones. | the preparation of a series of 6-acyl-3-substituted-2-(3h)-benzoxazolones is described. their structures have been elucidated by uv, ir, nmr, ms and elementary analysis. these compounds have been tested for their antimicrobial activities against to escherichia coli atcc 25922, staphylococcus aureus, atcc 25923, pseudomonas aeruginosa atcc 27853, staphylococcus faecalis rskk 10541, candida parapsilosis, candida albicans, candida pseudotropicalis, candida stellatoidea. | 1989 | 2621552 |
| polyphosphate synthesis in yeast. | polyphosphate synthesis was studied in phosphate-starved cells of saccharomyces cerevisiae and kluyveromyces marxianus. incubation of these yeasts for a short time with phosphate and either glucose or ethanol resulted in the formation of polyphosphate with a short chain length. with increasing incubation times, polyphosphates with longer chain lengths were formed. polyphosphates were synthesized faster during incubation with glucose than with ethanol. antimycin did not affect the glucose-induced ... | 1989 | 2643440 |
| restriction endonuclease analysis of mitochondrial dna from candida parapsilosis and other candida species. | mitochondrial dna has been studied in a number of eukaryotic organisms. differences in inter- and intraspecies mitochondrial dna restriction patterns have been shown to be due to differences in nucleotide sequences and have been used to study evolutionary relationships and the mode of inheritance of the mitochondrial genome. a relatively rapid and efficient method for the extraction of mitochondrial dna from candida parapsilosis and other candida species was developed. zymolyase was used to indu ... | 1989 | 2665367 |
| induction of murine macrophage fungal killing by interleukin 3. | the effect of recombinant interleukin 3 (il-3) on the function of murine resident peritoneal macrophages was investigated. il-3 enhanced the phagocytosis of candida pseudotropicalis and candida albicans and enhanced killing of the former. the enhanced killing is inhibited by scavengers of oxygen radicals, suggesting that il-3 primes macrophages for enhanced oxidative metabolism in response to candida. | 1989 | 2788580 |
| evaluation of cilofungin, a lipopeptide antifungal agent, in vitro against fungi isolated from clinical specimens. | cilofungin (ly121019) is a new lipopeptide antifungal drug. we tested this drug against 141 pathogenic fungal isolates. all fungal species were tested by broth dilution at 35 degrees c. malassezia furfur was tested by agar dilution. the results demonstrate the specificity of cilofungin activity. candida albicans, candida tropicalis, and malassezia pachydermatis were highly susceptible, whereas candida parapsilosis, candida pseudotropicalis, candida krusei, torulopsis glabrata, blastomyces dermat ... | 1989 | 2802565 |
| ester formation from ethanol by candida pseudotropicalis. | the production of ethanol, acetate ion and ethyl acetate from glucose by the yeast candida pseudotropicalis ncyc 143 was investigated under aerobic and anaerobic growth conditions. acetate and ethyl acetate only accumulated under aerobic conditions, whereas production of the alcohol was favoured by anaerobic conditions. ester production during aerobic growth was enhanced substantially by growth in iron-deficient media. possible conditions for optimising ester production from ethanol in dilute pr ... | 1989 | 2802574 |
| synthesis of some new mannich bases derived from 6-acyl-3-(3,5-dimethylpiperidinomethyl)-2(3h)-benzoxazolones+ ++ and their biological activities. | a number of 6-acyl-3-(3,5-dimethylpiperidinomethyl)-2(3h)-benzoxazolones have been synthesized by mannich reaction and their antibacterial and antifungal activities have been tested. their chemical structures have been proved by means of their ir, nmr, mass spectroscopic data and by elementary analysis. investigation of antimicrobial activity of compounds was done by tube dilution and paper disc techniques using bacteria (escherichia coli atcc 25922, staphylococcus aureus atcc 25923, pseudomonas ... | 1989 | 2818673 |
| sequence and transcription of the beta-glucosidase gene of kluyveromyces fragilis cloned in saccharomyces cerevisiae. | the complete nucleotide sequence of the beta-glucosidase gene of kluyveromyces fragilis has been determined. this sequence contains an open reading frame of 2535 base pairs encoding a protein of 845 amino acids. analysis of the transcription products revealed only one transcript of about 3 kb identical in both kluyveromyces fragilis and in the expression host saccharomyces cerevisiae. the protein molecular weight of 93,811 kd deduced from the sequence is consistent with the 90,000 kd determined ... | 1987 | 2835179 |
| mitochondrial dna of the yeast kluyveromyces: guanine-cytosine rich sequence clusters. | mitochondrial dna from the yeast kluyveromyces marxianus var. lactis (k.lactis) is a circular molecule of 39 kilobase-pairs. a genetic and physical map was constructed. we found that this genome contained a large number of guanine-cytosine (gc)-rich sequence clusters, many of which are characterized by the presence of sacii restriction sites (ccgcgg). the primary sequence of the gc clusters often showed a palindromic structure. these gc clusters were present in several varieties of k.marxianus, ... | 1988 | 2843818 |
| arrangement of genes trp1 and trp3 of saccharomyces cerevisiae strains. | the tryptophan biosynthetic genes trp1 and trp3 and partly also trp2 and trp4 have been compared by the technique of southern hybridization and enzyme measurements in twelve wild isolates of saccharomyces cerevisiae from natural sources of different continents, in the commonly used laboratory strain s. cerevisiae x2180-1a and in a kluyveromyces marxianus strain. we could classify these strains into four groups, which did not correlate with their geographical distribution. in no case are the trp3 ... | 1985 | 2998296 |
| early defect of phagocytic cell function in subjects at risk for acquired immunodeficiency syndrome. | we studied the functions of peripheral blood monocytes and polymorphonuclear cells in 15 apparently healthy homosexual men, eight homosexual or bisexual subjects with unexplained generalized lymphadenopathies (pre-aids), four homosexual men with acquired immunodeficiency syndrome (aids), and 15 heterosexual men. in comparison with normal controls, the homosexual groups studied presented a decreased monocyte candidacidal activity for candida pseudotropicalis that gradually deteriorates as the cli ... | 1986 | 3018916 |
| mitochondrial introns as mobile genetic elements: the role of intron-encoded proteins. | introns of organelle genes share distinctive rna secondary structures that allow their classification into two known families. these structures are believed to play an essential role in splicing, and members of both structural classes have recently been shown to perform self-splicing reactions in vitro. in lower eukaryotes, many structured introns also contain long internal open reading frames (orfs), which are able to code for hydrophilic proteins. several properties of self-splicing structured ... | 1986 | 3032144 |
| molecular probe for identification of medically important candida species and torulopsis glabrata. | a cloned dna fragment from candida albicans containing the gene for the protein actin was used to probe the molecular structure of the actin gene of several medically important yeasts (c. albicans, candida stellatoidea, candida tropicalis, candida pseudotropicalis, candida krusei, candida parapsilosis, candida guilliermondii, and torulopsis glabrata). whole-cell dna from each species was digested with restriction endonucleases, electrophoresed on agarose gels, and transferred to nitrocellulose. ... | 1987 | 3033014 |
| the role of atp in the control of h+-galactoside symport in the yeast kluyveromyces marxianus. | transport of methyl beta-d-thiogalactoside and p-nitrophenyl beta-d-galactoside is shown to proceed through the h+-lactose symporter of kluyveromyces marxianus. uptake of these compounds is strongly reduced under anaerobic conditions or aerobically in the presence of antimycin. it is shown that antimycin treatment affects p-nitrophenyl beta-d-galactoside uptake in a similar way as it affects the cellular amount of atp, suggesting regulation of p-nitrophenyl beta-d-galactoside transport by atp. a ... | 1987 | 3036099 |
| ofage banding patterns of different yeast genera and of intergeneric hybrids. | electrophoretic karyotypes of yeasts belonging to the species saccharomyces cerevisiae, kluyveromyces marxianus and candida macedoniensis were established by means of ofage. hybrids between s. cerevisiae and k. marxianus as well as between k. marxianus and c. macedoniensis were analyzed by comparing their ofage-banding-pattern with the parental banding-patterns. thus, evidence for exchanges of intact chromosomes and for chromosomal rearrangements could be gained on a molecular level. | 1988 | 3057175 |
| lysosomal enzyme release from macrophages: a model of food yeast toxicity evaluation. | the role of lysosomal enzyme released by macrophages was examined in relation to the toxic effect caused by food yeast. mouse peritoneal macrophages exposed to yeast in culture showed marked release of n-acetyl glucosaminidase, beta-galactosaminidase and beta-glucuronidase below the median lethal dose (ld50). ld50 was measured from the dose response curves of the cytoplasmic lactate dehydrogenase enzyme. saccharomyces cerevisiae showed the highest ld50 followed by kluyveromyces fragilis and cand ... | 1988 | 3076734 |
| biosynthesis regulation of the beta-glucosidase produced by a yeast strain transformed by genetic engineering. | the biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in saccharomyces cerevisiae the structural gene coding for beta-glucosidase in kluyveromyces fragilis. the enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. these features are similar to those observed in k. fragilis. beta-glucosidase activity in the transformed yeast was much higher than in k. fragilis. we attempted to ferment cellobiose with the transformed yeast: ... | 1986 | 3099720 |
| the promoter of the beta-glucosidase gene from kluyveromyces fragilis contains sequences that act as upstream repressing sequences in saccharomyces cerevisiae. | the relationship between the promoter length of the kluyveromyces fragilis beta-glucosidase gene and the level of its expression in saccharomyces cerevisiae was studied by gene fusion between deleted promoter fragments of various lengths and the promoterless beta-galactosidase gene of escherichia coli. the removal of a region from position -425 to -232 led to a tenfold increase in the expression of the gene. the same results were obtained for the reconstructed beta-glucosidase gene with the same ... | 1988 | 3146687 |
| in situ assay of intracellular enzymes of yeast (kluyveromyces fragilis) by digitonin permeabilization of cell membrane. | the yeast, kluyveromyces fragilis was permeabilized to a number of low-molecular-weight substrates using digitonin. the activities of intracellular yeast enzymes, viz., alcohol dehydrogenase (adh), beta-galactosidase, glucose-6-phosphate dehydrogenase, aspartase, and hexokinase were found to be much higher in the permeabilized cells than the untreated cells. the optimum conditions for permeabilization with reference to adh were 0.1% digitonin at 37 degrees c for 15 min. the adh activity in perme ... | 1988 | 3149161 |
| comparison of the properties of the purified beta-glucosidase from the transformed strain of saccharomyces cerevisiae tykf2 with that of the donor strain kluyveromyces fragilis y610. | saccharomyces cerevisiae tykf2 was obtained by cloning in s. cerevisiae the gene coding for beta-glucosidase in kluyveromyces fragilis y610 (atcc 12424). the beta-glucosidases of both organisms were purified and their biochemical characteristics were determined. the two beta-glucosidases had the same enzymatic properties as those previously described in the literature. the strain s. cerevisiae tykf2 is able to produce enhanced amounts of enzyme. | 1987 | 3314909 |
| predictive value of surveillance cultures for systemic infection due to candida species. | weekly fungal surveillance cultures (1,542 cultures) of urine (475), stool (520) and oropharyngeal (547) specimens from 111 patients on the bone marrow transplant and hematologic malignancy services were analyzed. forty-three percent of the patients were colonized by candida albicans and 10.8% by candida tropicalis. there were 22 proven systemic fungal infections, ten due to candida albicans, eight to candida tropicalis, one each to candida pseudotropicalis and torulopsis glabrata, and two to as ... | 1987 | 3326742 |
| immunological homologies between ribosomal proteins amongst lower eukaryotes. | polyclonal antibodies were raised against the purified ribosomal proteins l1 and l2, the 5s rrna binding protein l3, all from saccharomyces cerevisiae, and against l1 and l2 from schizosaccharomyces pombe (numbering according to otaka and osawa 1981; otaka et al. 1983, respectively). for clarity prefixes sc and sp have been added to the numbering of proteins derived from s. cerevisiae and s. pombe, respectively. ribosomal proteins from these yeasts and from kluyveromyces marxianus, rhodotorula g ... | 1986 | 3327609 |
| [antibacterial and antifungal compounds. viii. synthesis and antifungal activity of pyrrol derivatives similar to trichostatin a]. | some p-methylbenzolpyrrole acrylic acids and related compounds were synthesized. the new pyrrole derivatives have structural features in common with trichostatin a, an antifungal antibiotic. the above acids and derivatives were tested against candida albicans and candida sp in comparison with miconazole, pyrrolnitrin and amphotericin b and showed very weak antifungal activities. occasionally some activity was found against a few strains of candida albicans and against candida pseudotropicalis. | 1987 | 3329612 |
| regulation of sugar transport systems of kluyveromyces marxianus: the role of carbohydrates and their catabolism. | in kluyveromyces marxianus grown on a glucose-containing synthetic medium four different sugar transporters have been identified. in cells, harvested during the exponential phase, only the constitutive glucose/fructose carrier, probed with 6-deoxy-d-glucose or sorbose, appeared to be active. in cells from the stationary phase three proton symporters can be active, recognizing 6-deoxyglucose (a glucose/galactose carrier), sorbose (a fructose carrier) and galactosides (lactose carrier), respective ... | 1988 | 3355832 |
| impairment of insulin assimilation and beta-fructosidase activity due to a petite mutation in kluyveromyces marxianus. | a respiratory deficient mutant of kluyveromyces fragilis was isolated using ethidium bromide mutagenesis. it was characterized by a loss of cytochromes a + a3 and deficiency in cytochrome b. this petite mutant has brought about modifications in the excretion pattern of beta-fructosidase active on saccharose and inulin. the mutant practically no longer excretes the enzyme, and is incapable of growth and fermentation in the presence of inulin. the study of the activities of different enzyme extrac ... | 1988 | 3395109 |
| monocyte-macrophage (m-m) functions in asymptomatic hemophiliacs and supertransfused thalassemics. | patients suffering from acquired immune deficiency syndrome (aids) succumb to opportunistic infections due to a generalized failure of their cell-mediated immune defenses. the monocyte-macrophage (m-m) system plays an important role in host defense against viruses, protozoa, mycobacteria, and tumours, all potentially involved in the terminal stages of aids. we studied m-m functions in 55 asymptomatic hemophiliacs, 20 supertransfused thalassemics, and 9 von willebrand's syndrome patients over a p ... | 1987 | 3498605 |
| kluyveromyces fragilis ss-437: an associatively-profiled thermotolerant yeast. | the lactose-utilizing kluyveromyces fragilis ss-437 was found to have an associative temperature profile, but a thermotolerant growth yield behaviour. cardinal growth temperatures were: 3 degrees c minimum for growth; 41.5 degrees c optimum; 44.5 degrees c final maximum (growth and death rates equalize); 46.1 degrees c initial maximum (maximum limit for growth). | 1987 | 3662480 |
| characterization of low- and high-affinity glucose transports in the yeast kluyveromyces marxianus. | glucose transport in the yeast kluyveromyces marxianus proceeds by two functionally and presumably structurally distinct transporters depending on the carbon source of the culture medium. in lactose-grown cells, glucose was taken up through a high-affinity h+-sugar symporter (km = 0.09 mm), whereas a low-affinity transporter (km = 3.5 mm) was utilized in glucose-grown cells. the two transporters exhibited different substrate specificities. galactose was demonstrated to be a selective substrate o ... | 1987 | 3663655 |
| [optimizing conditions for the discontinuous production of unicellular protein using whey]. | the cheese whey is one of the most important effluents which is being disposed off in our area. that is why the study for optimizing conditions in the process of recovering whey to produce protein biomass in a batch fermenter was undertaken. a yeast strain (kluyveromyces marxianus var. lactis) was propagated on a medium based on whey from cheese making plants, the following conditions for cell development being optimized: temperature, ph and initial concentration of lactose. a methodology based ... | 1987 | 3685391 |
| evaluation of the new mycotube test-kit for yeast identification. | the performance of a modified mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. one hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. the overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features were also considered; a 100% rate was obtained with candida albicans, candida krusei, candida parapsilosis, candida pseudotropicalis, candid ... | 1985 | 3886377 |
| [various characteristics of growth kinetics and exohydrolase synthesis in aspergillus foetidus and zygofabospora marxiana]. | the kinetics of growth and synthesis of exohydrolases (polygalacturonases and proteinases) were studied in aspergillus foetidus and zygofabospora marxiana. the processes of growth and synthesis of polygalacturonases were found to be shifted in time. endopolymethylgalacturonase of the micromycete and endopolygalacturonase of the yeast are not accumulated in the cells, but are mainly secreted into the growth medium. the maximal value of polygalacturonase bound to the cell coincides with the maxima ... | 1985 | 3892247 |
| effect of mycolase and amphotericin b on candida albicans and candida pseudotropicalis in vitro and in vivo. | a mixture of enzymes (mycolase) capable of lysing yeast cell walls was prepared from culture filtrates of physarum polycephalum. the enzymes present in mycolase included chitinase, beta-1,3-glucanases and exo-glycosidases. the ph optima of these enzymes were in the range 3.5-5.0 and they had low activities at ph 7.0. mycolase produced spheroplasts from candida pseudotropicalis and, unlike commercial enzyme preparations such as l1, chitinase, beta, 1,3-glucanase and beta-glucosidase, had some can ... | 1985 | 3895468 |
| normalization of monocyte candidacidal deficiency by cyclooxygenase inhibitors in hodgkin's disease. | in a previous work, the authors found that the peripheral blood monocytes from patients with hodgkin's disease (hd) had depressed lytic capability to kill candida pseudotropicalis and depressed phagocytic function. the aim of this study was to evaluate if cyclooxygenase inhibitors could correct the defective macrophage functions. fifteen untreated patients with hd and 10 normal subjects were studied. the incubation of the cells from the patients with hd with indomethacin (im) at 1, 3, and 10 mic ... | 1985 | 3922613 |