| some properties of proteases of the mould mucor pusillus-917. | the proteolytic preparation of mucor pusillus-917 has been obtained. the preparation produces an effective hydrolytic influence on milk casein. thermal and acid inactivation of the proteolytic complex is the reaction of the first order. the activity-ph curves, calculations of ionization heat, inactivation of proteases by photooxidation and monoiodoacetic acid suggest that imidazole, carboxyl and sulphydryl groups of proteases are involved in the clotting and hydrolysis of milk. | 1975 | 1731 |
| the structure and function of acid proteases. iv. inactivation of the acid protease from mucor pusillus by acid protease-specific inhibitors. | mucor pusillus acid protease was rapidly inactivated with 1 : 1 stoichiometry by reaction with diazoacetyl-dl-norleucine methyl ester (dan) in the presence of cupric ions. cupric ions were essential for this inactivation. the rate of inactivation was maximal at around ph 6 when the enzyme was mixed with dan and cupric ions without prior mixing of the reagents, and at ph 5.3 when dan and cupric ions were mixed and incubated before addition to the enzyme solution. in both cases, the rate of inacti ... | 1976 | 9381 |
| the structure and function of acid proteases. v. comparative studies on the specific inhibition of acid proteases by diazoacetyl-dl-norleucine methyl ester, 1,2-epoxy-3-(p-nitrophenoxy) propane and pepstatin. | comparative studies have been made on the effects of diazoacetyl-dl-norleucine methyl ester (dan), 1,2-epoxy-3-(p-nitrophenoxy)propane (epnp) and pepstatin on acid proteases, including those from acrocylindrium sp., aspergillus niger, aspergillus saitoi, mucor pusillus, paecilomyces varioti, rhizopus chinensis, and trametes sanguinea, and also porcine pepsin ec 3.4.23.1 and calf rennin ec 3.4.23.4 for comparative purposes. these enzymes were rapidly inactivated at similar rates and in 1:1 stioch ... | 1976 | 10290 |
| development and evaluation of an immunodiffusion test for diagnosis of systemic zygomycosis (mucormycosis): preliminary report. | an antigen analysis with filtrate and homogenate precipitinogens of single isolates of the zygomycetes absidia corymbifera, mucor pusillus, rhizopus arrhizus, and rhizopus oryzae demonstrated the presence of common antigens among the three genera as well as antigens which permit their differentiation. selected homogenate antigens were valuable in developing a diagnostic immunodiffusion (id) test for systemic zygomycosis. when sera from 43 patients with various proven mycoses other than zygomycos ... | 1978 | 75212 |
| cutaneous infarction. manifestation of disseminated mucormycosis. | we describe the second reported case of cutaneous infarction as a manifestation of disseminated mucormycosis. the lesion, which closely resembled ecthyma gangrenosum, occurred in a leukemic patient who was on a regimen of broad-spectrum antibiotics. in this case as in the only other reported case, mucor pusillus was the cause. | 1977 | 268159 |
| action of milk clotting enzymes on alphas-caseins from buffalo's and cow's milk. | alphas-caseins were isolated from buffalo's and cow's milk and hydrolyzed with rennet, bovine pepsin, microbial proteases from mucor miebei, mucor pusillus lindt, and endotbia parasitica. the rate of hydrolysis was followed by determining the unaltered alphas-casein in the digest after acrylamide gel electrophoresis. the rate of hydrolysis of alphas-casein from the two species with the different enzymes was comparable, being more rapid with microbial enzymes particularly endotbia parasitica prot ... | 1977 | 334809 |
| thermophilic and thermotolerant fungi in poultry droppings in nigeria. | ten species of fungi were obtained from poultry droppings in nigeria. six of these are true thermophiles while the other four are thermotolerant. aspergillus fumigatus fresenius, mucor pusillus lindt and thermoascus aurantiacus stolk are known human pathogens. except for m. pusillus, all the thermotolerant species had a higher occurrence at 45 degrees c while the thermophilic varieties were readily obtained at 50 degrees c. | 1979 | 393797 |
| comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi. | temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the arrhenius plots were linear between 5 degrees and 50 degrees. the energies of activation ranged from 2.1 kcal/mol for the enzyme from sporotrichum thermophile to 9.1 kcal/mol for that from penicillium duponti. heat inactivation kinetics at 50 degrees revealed hea ... | 1979 | 489257 |
| stimulation of growth and glucose catabolite enzymes by succinate in some thermophilic fungi. | thermophilic humicola lanuginosa, penicillium duponti, sporotrichum thermophile and mucor pusillus required succinate in addition to glucose for optimal growth. the requirement for succinate was concentration-dependent and the concentration needed for one half of the maximal growth was 6.14mm. in the presence of succinate, glucose utilization from the medium was markedly increased and this was associated with increased levels of the enzymes of the glycolytic and krebs cycle pathways. addition of ... | 1978 | 697502 |
| [cerebral mucormycosis in the cat caused by mucor pusillus]. | the pathologic examination of the brain of a pet cat, suspected of rabies, showed lesions of mucormycosis. the causal fungus, mucor (rhizomucor) pusillus was isolated and identified. the authors describe the lesions produced, the experimental pathogenicity for the rabbit and the morphologic and physiologic characteristics of the isolate. | 1978 | 752192 |
| the primary structure of sillucin and antimicrobial peptide from mucor pusillus. | | 1979 | 761621 |
| the relative susceptibility of new zealand black and cba mice to infection with opportunistic fungal pathogens. | the susceptibility of ageing new zealand black (nzb) mice to infection with a number of opportunistic fungi and yeasts wsd compared with that of similarly aged mice of the cba strain. the nzb mice showed a greater susceptibility to lethal infection with aspergillus fumigatus, candida albicans and cryptococcus neoformans than did those of the cba strain. both mouse strains showed similar susceptibility to lethal infection by allescheria boydii, absidia corymbifera, mucor pusillus and rhizopus ory ... | 1976 | 772839 |
| induced azygospore formation in mucor (rhizomucor) pusillus by absidia corymbifera. | | 1976 | 1085125 |
| mucormycosis (mucor pusillus) with asteroids in a young bull. | | 1975 | 1181908 |
| keratinolysis by absidia cylindrospora and rhizomucor pusillus: biochemical proof. | absidia cylindrospora and rhizomucor pusillus causal agents of phycomycoses, were cultured on sterile natural keratins in a mineral solution and the keratin degradation products analyzed. the excess of sulphur was removed by oxidation to inorganic sulphate and thiosulphate, which were the main products of sulphitolysis of keratin. the proteolytic activity of the two fungi depended on the nature of the keratin substrate. human scalp hair was the most favoured keratin substrate by both the fungi. | 1992 | 1279428 |
| [inhibitory effects of organic acids and salts on selected micromycetes]. | inhibitory effects of two fungistatic preparations (a and b) and of propionic acid were tested in a complete feed mixture for broiler chickens (starter mixture). the water content of this mixture was 25.4%, environmental temperature was 24 degrees c and relative air humidity 90%. propionic acid, which has a high fungistatic effect, served as a standard. the composition of the preparation a was as follows: propionic acid, acetic acid, sorbic acid, citric acid and calcium propionate. the preparati ... | 1992 | 1297244 |
| protein engineering of the milk-clotting aspartic proteinases. | calf chymosin and a fungal protease from mucor pusillus (mucor rennin) are members of the aspartic proteinases used as milk-coagulants in cheese industry. a system for production of recombinant chymosin as inclusion bodies in escherichia coli cells and its refolding into the active form was established. another expression system for production of mucor rennin in saccharomyces cerevisiae was also established. mucor rennin was efficiently excreted from the yeast host as a heavily glycosylated form ... | 1992 | 1455180 |
| fungi associated with bovine abortion in the northern plains states (usa). | mycotic infection was diagnosed in 6.8% of 6,858 cases of bovine abortion and stillbirth examined during a 9-year period. aspergilli were associated with approximately 5% of all abortion cases and 71% of 446 cases that were cultured for fungi and diagnosed as mycotic abortion. aspergillus fumigatus was the most frequent isolate (62%), followed by a. terreus (6.7%), emericella (aspergillus) nidulans (3.0%), a. flavus (2.9%), and e. rugulosus (less than 1.0%). zygomycetes (absidia, mortierella, rh ... | 1992 | 1616983 |
| thermophilic and thermotolerant fungi of animals' hair. | nine thermophilic genera and 17 species in addition to one variety of aspergillus flavus, malbranchea pulchella and humicola grisea were collected from hair samples in riyadh, saudi arabia at 45 degrees c. fifty-one hair specimens of rabbit, sheep, camel and horse were examined for the presence of thermophilic fungi. the most frequent species were aspergillus fumigatus, aspergillus niger, thermoascus aurantiacus and malbranchea pulchella var. sulfurea. in low frequency, aspergillus flavus, asper ... | 1991 | 1805498 |
| the secretion leader of mucor pusillus rennin which possesses an artificial lys-arg sequence directs the secretion of mature human growth hormone by saccharomyces cerevisiae. | the prepro-peptide of fungal aspartic proteinase, mucor pusillus rennin, is useful as a secretion leader for efficient secretion of human growth hormone (hgh) from saccharomyces cerevisiae. for secretion by yeast cells of hgh with the same nh2 terminus as native hgh, an artificial lys-arg linker, which is one of the potential kex2 recognition sequences, was introduced at the junction between the m. pusillus rennin secretion leader and mature hgh. the hgh directed by this construction was the sam ... | 1991 | 1892395 |
| isolation and characterization of human pro-urokinase and its mutants accumulated within the yeast secretory pathway. | human pro-urokinase (pro-uk) and two pro-uk deletion mutants, one lacking the epidermal growth factor(egf)-like domain, and the other lacking both the egf-like domain and the kringle domain, were produced in saccharomyces cerevisiae. this was done using the yeast gal7 promoter and the prepeptide sequence of a fungal aspartic proteinase, mucor pusillus rennin (mpr). although biologically active and heavily glycosylated pro-uks were secreted into the culture medium, the amounts were extremely smal ... | 1991 | 1902432 |
| systemic zygomycosis: nosocomial infection by rhizomucor pusillus. | | 1991 | 2034262 |
| effects of glycosylation on the secretion and enzyme activity of mucor rennin, an aspartic proteinase of mucor pusillus, produced by recombinant yeast. | the mucor rennin gene encoding a prepro form of the fungal aspartic proteinase from mucor pusillus was expressed under the control of the yeast gal7 promoter in saccharomyces cerevisiae. the mature m. pusillus rennin secreted efficiently by yeast was a highly glycosylated protein. analysis by a combination of site-directed mutagenesis of each of the three possible glycosylation sites and treatment of the secreted m. pusillus rennins with endo-beta-n-acetylglucosaminidase h revealed that the matu ... | 1990 | 2116411 |
| the prepro-peptide of mucor rennin directs the secretion of human growth hormone by saccharomyces cerevisiae. | an aspartic proteinase, mucor pusillus rennin (mpr), of filamentous fungus mucor pusillus, is efficiently secreted from a transformant of saccharomyces cerevisiae containing the intact mpr gene. to test the usefulness of the mpr leader peptide in secretion of heterologous proteins from yeast cells, several plasmids encoding the fusion proteins composed of different parts of the nh2-terminal region of prepro-mpr and human growth hormone (hgh) were constructed. the parts of the leader peptide upst ... | 1990 | 2117879 |
| fungal flora associated with combine harvester wheat and sorghum dusts from egypt. | 107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-czapek's dox agar at 28 degrees c and 45 degrees c. the mycoflora of wheat and sorghum dusts were basically similar on the two types of media and the most common fungi were: alternaria alternata, aspergillus flavus, a. fumigatus ... | 1990 | 2266490 |
| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| [reactivity of human lymphocytes to yeast-like and mold fungi]. | the capacity of the allergens of mold fungi (rhizopus nigricans, mucor pusillus, alternaria tenuis, cladosporum herbarum, fusarium oxysporum) and yeast-like fungi (candida albicans) to induce the production of lymphokins by human lymphocytes was studied. all these preparations were active in reactions with lymphocytes obtained from adult donors, but did not activate lymphocytes of newborns (obtained from umbilical blood). in equal doses (10 micrograms/ml) c. albicans allergen was more active tha ... | 1989 | 2500001 |
| secretion by yeast of the zymogen form of mucor rennin, an aspartic proteinase of mucor pusillus, and its conversion to the mature form. | the mucor rennin gene encoding a prepro-form of the fungal aspartic proteinase from mucor pusillus was expressed under the control of the yeast gal7 promoter in saccharomyces cerevisiae. an inactive zymogen of the enzyme with the 44-amino-acid pro-sequence was identified in the medium during the initial stage of cultivation. processing of the purified zymogen to the mature enzyme proceeded autocatalytically under the acidic conditions. the rate of processing was accelerated by an increase in the ... | 1989 | 2506185 |
| fungal flora of poultry feedstuff ingredients. | one hundred and ten samples representing five types of poultry feed ingredients were mycologically examined. these samples included soybean meal, ground maize, cotton-seed cake, wheat bran and fish meal (22 samples each). among the 73 mesophilic fungal species, aspergillus flavus, a. niger and a. fumigatus were the most dominant. a. terreus, a. flavipes, mucor circinelloides, scopulariopsis brevicaulis, penicillium chrysogenum, fusarium moniliforme and rhizopus stolonifer were found to be common ... | 1989 | 2621568 |
| indirect enzyme-linked immunosorbent assay for zygomycosis. | a 2-h indirect enzyme-linked immunosorbent assay (elisa) using homogenate antigens of rhizopus arrhizus and rhizomucor pusillus was developed and compared with the existing immunodiffusion (id) test for zygomycosis, using homogenate antigens of r. arrhizus. utilizing 1:400 as a minimally positive elisa titer, 33 of 43 proven cases of zygomycosis were diagnosed. the sensitivity of the elisa was 81%. the id test, in contrast, detected only 21 cases and demonstrated a sensitivity of 66%. the specif ... | 1989 | 2674194 |
| storage temperature and viability of sporangiospores of potentially human pathogenic species of rhizomucor from nigerian tobacco. | the effects of temperature on storage and viability of asexual spores of rhizomucor pusillus (lindt) schipper and rhizomucor miehei (cooney and emerson) schipper were studied. an optimal germination temperature of 35 degrees c was recorded for both species with good results obtained around 40 degrees c. on prolonged storage of the spores at various temperatures (-19 degrees c, 4 degrees c, 30 degrees c, 37 degrees c), the best results were obtained at 4 degrees c and 30 degrees c. very rapid los ... | 1989 | 2746473 |
| cellulitis caused by rhizomucor pusillus in a diabetic patient receiving continuous insulin infusion pump therapy. | we have reported a case of primary cutaneous zygomycosis in a diabetic patient receiving continuous insulin infusion pump therapy. we believe this to be the first reported case of a fungal infection associated with the use of an insulin pump. the infection itself was an unusually mild and chronic zygomycosis, possibly due to a low production rate of proteolytic enzymes by a rare opportunistic human pathogen, rhizomucor pusillus. | 1989 | 2814631 |
| protein chemical characterization of mucor pusillus aspartic proteinase. amino acid sequence homology with the other aspartic proteinases, disulfide bond arrangement and site of carbohydrate attachment. | the amino acid sequence of mucor pusillus aspartic proteinase was determined by analysis of fragments obtained from cleavage of the enzyme by cnbr and limited tryptic digestion. the proteinase is a single polypeptide chain protein containing 361 amino acid residues, cross-linked by two disulfide bonds. a sugar moiety composed of two glcnac residues and four neutral sugar residues is asparagine-linked to the chain. the sequence of m. pusillus proteinase is highly homologous with the m. miehei pro ... | 1988 | 3042459 |
| secretion of mucor rennin, a fungal aspartic protease of mucor pusillus, by recombinant yeast cells. | the aspartic protease gene of a zygomycete fungus mucor pusillus was expressed in saccharomyces cerevisiae under the control of the yeast gal7 promoter. a putative preproenzyme with an nh2-terminal extension of 66 amino acids directed by the gene was processed in yeast cells and the mature enzyme, whose nh2-terminus was identical to that of the mucor enzyme, was efficiently secreted into the medium at a concentration exceeding 150 mg/l. the enzyme secreted from the recombinant yeast was more gly ... | 1987 | 3323844 |
| primary structure of a precursor to the aspartic proteinase from rhizomucor miehei shows that the enzyme is synthesized as a zymogen. | in order to characterize the zymogen of the milk-clotting enzyme from rhizomucor miehei, we constructed a cdna library on pbr327 in escherichia coli. aspartic proteinase-specific recombinants were isolated by colony hybridization to a specific oligonucleotide mixture, and the cdna sequence corresponding to a precursor form of the enzyme was determined. the deduced amino acid sequence shows that this secreted fungal proteinase is synthesized as a precursor. the first 22 amino acid residues in thi ... | 1986 | 3329734 |
| cloning and sequencing of a gene for mucor rennin, an aspartate protease from mucor pusillus. | the aspartate protease of mucor pusillus (mucor pusillus rennin; mpr) is a milk-clotting enzyme used in the cheese industry. the partial amino acid sequence of mpr was determined and oligonucleotide probes were synthesized for cloning of the mpr gene. a clone giving positive hybridization with the probes was selected from the cosmid library. sequencing of the cloned dna revealed an open reading frame of 1281 bp without introns which encodes 361 amino acids for the expected mpr with an nh2-termin ... | 1986 | 3534790 |
| inactivation of acid proteases from rhizopus chinensis, aspergillus saitoi and mucor pusillus, and calf rennin by diazoactylnorleucine methyl ester. | | 1972 | 4552474 |
| x-ray crystallographic study of the rennin-like enzyme of mucor pusillus var. lindt. | | 1972 | 4560757 |
| [ultilization of microbial proteases in the dairy industry. 1. studies of the conditions for the production of the milk-coagulating enzyme by endothia parasitica and mucor pusillus]. | | 1972 | 4679137 |
| kinetic studies on the aggregation of kappa-casein by mucor pusillus protease. | | 1973 | 4702007 |
| x-ray inactivation in solution of rennin from mucor pusillus. | | 1973 | 4739670 |
| [optimization of nutrient medium composition during cultivation of mucor pusillus 917]. | | 1973 | 4805936 |
| synthesis of cellulase by mucor pusillus and mucor miehei. | | 1974 | 4822120 |
| milk-clotting enzyme from microorganisms. vi. properties of crystalline milk-clotting enzyme (mucor rennin) isolated from mucor pusillus var. lindt. | | 1969 | 4884139 |
| inhibition of gram-positive bacteria by mucor pusillus. | | 1969 | 4984377 |
| antimicrobial polypeptide synthesized by mucor pusillus nrrl 2543. | | 1970 | 4985140 |
| purification and properties of mucor pusillus acid protease. | the protease produced by mucor pusillus was recovered from a wheat bran medium by treatment with ammonium sulfate, ethyl alcohol, gel filtration and ion-exchange chromatography. the yield of the enzyme was 55%. the overall increase in the specific activity of the protease was 34-fold. the purified protease was most active at ph 3.8 and 5.6 against hemoglobin and casein, respectively. optimal hydrolysis of casein was observed at 55 c. the enzyme was stable from ph 3.0 to 6.0. enzyme inactivated b ... | 1968 | 5646628 |
| acid protease synthesis by mucor pusillus in chemically defined media. | the acid protease synthetized by mucor pusillus in chemically defined medium displayed a gel filtration pattern and optimal ph similar to that shown by protease isolated from complex medium. protease synthesis was influenced by the concentration of sulfur in the medium. at sulfur concentrations greater than 10(-4)m, protease synthesis was suppressed, indicating a controlling role of sulfur compounds in the synthesis of extracellular protein. inorganic phosphate was found to be without negative e ... | 1968 | 5646629 |
| lipase activity of mucor pusillus. | two strains of mucor pusillus were examined for their ability to synthesize lipase in a complex medium used in the production of milk-clotting protease. lipase activity of both strains reached maximal after 6 days of incubation under submerged conditions at 35 c. lipase secreted into the medium hydrolyzed butterfat and vegetable lipids, as well as selected synthetic triglycerides. about 50% of lipase activity was destroyed after a 45-min heat treatment at 58 c. | 1968 | 5647521 |
| lipase of mucor pusillus. | lipase of mucor pusillus nrrl 2543 was recovered with ammonium sulfate precipitation, gel filtration on sephadex g-75, and anion-exchange chromatography on diethylaminoethyl-sephadex a-50. maximal glycerol ester hydrolase (lipase) activity was observed at ph 5.0 to 5.5 and 50 c when trioctanoin and olive oil were used as substrates. the enzyme also showed esterase activity; it hydrolyzed, with the exception of methyl butyrate, all methyl esters tested. a minimum chain length of six carbons appea ... | 1969 | 5772396 |
| production of mucor pusillus acid protease in reconstituted whey. | | 1969 | 5778298 |
| cellulolysis by mucor pusillus. | culture filtrates of mucor pusillus nrrl 2543 contained hydrolytic enzymes that attacked native cellulose, acid-swollen cellulose, carboxymethylcellulose, and cellobiose. the distribution profiles of cellulolytic and beta-glucosidase activities after gel filtration on sephadex g-75 showed the presence of several active peaks. glucose was the only product of hydrolysis when native cellulose was used as the substrate. acid-swollen cellulose, when treated with cellulase free of beta-glucosidase act ... | 1969 | 5797941 |
| conditions influencing the synthesis of acid protease by mucor pusillus lindt. | protease synthesis by mucor pusillus lindt, in a wheat bran medium under submerged conditions, was influenced by substrate concentration, initial ph of the medium, and temperature of incubation. a 4% wheat bran (dry weight) concentration was satisfactory for enzyme production. the initial ph of the medium had a substantial effect on enzyme synthesis; adjustment of the enzyme production medium to ph 5.0 prior to sterilization was desirable. incubation at 35 c resulted in the best enzyme yields. u ... | 1967 | 6080716 |
| nosocomial infection by rhizomucor pusillus in a clinical haematology unit. | three patients suffering from acute leukaemia were treated with cytotoxic agents and broad-spectrum antibiotics and received blood transfusion and nasal packing for severe epistaxis. all developed necrosis of nasal and facial tissues, with facial swelling an oedema; two biopsies showed typical phycomycete mycelium, and rhizomucor pusillus was grown from one biopsy. air and surfaces in the unit and the air intake and ducting were all heavily colonized by rh. pusillus and other phycomycetes. it is ... | 1983 | 6190884 |
| immunodiffusion and complement fixation assays with sera from mucormycotic-infected mice. | results using homogenate antigens of rhizomucor pusillus against immunized rabbit sera, mouse ascites fluid and sera from mucormycotic-infected mice, detected by immunodiffusion and complement fixation assays are presented. using immunodiffusion techniques, common antigens and unique antigens from rh. pusillus, rhizopus oryzae and absidia corymbifera were observed. complement fixation titers of greater than 256 and 128 were obtained in immunized rabbit sera and mouse ascites fluid, respectively. ... | 1983 | 6422303 |
| passive immunization in murine mucormycosis. | antibody raised in mice against mycelial homogenates of rhizomucor pusillus was effective in passive immunization against pulmonary and disseminated mucormycosis (phycomycosis) in immunocompromised mice. mice intranasally inoculated and infected with rh. pusillus and treated with antisera had a statistically significant increased resistance to infection and a diminished secondary dissemination of viable fungal fragments. histological examination of infected lung tissues showed that antibody trea ... | 1983 | 6669169 |
| mucormycotic infection in mice following prolonged incubation of spores in vivo and the role of spore agglutinating antibodies on spore germination. | following intranasal inoculation of mice, rhizomucor pusillus spores are gradually cleared from the lung, with the clearance complete at 30 days. incubation of r. pusillus spores in vivo for up to 14 days after intranasal inoculation resulted in pulmonary mucormycosis when the mice were then treated with cortisone. spore-agglutinating igm antibodies were detected in normal adult mice and r. pusillus-inoculated but not cortisone-treated mice. there was no correlation between antispore antibody ti ... | 1984 | 6729658 |
| kinetic studies on the action of mucor pusillus, mucor miehei acid proteases and chymosins a and b on a synthetic chromophoric hexapeptide. | the action of two milk-clotting fungal proteases from mucos pusillus and mucor miehei and of chymosins a and b on the hexapeptide, leu-ser-phe(no2)-nle-ala-leu-ome, and on kappa-casein were studied. the effects of ph and temperature on the initial rates of hydrolysis of the hexapeptide were examined. crystalline chymosin and m. pusillus protease exhibited optimal activities around 49 and 55 degrees c, respectively, whereas the optimum temperature for m. miehei protease is higher than 63 degrees ... | 1980 | 6768392 |
| experimental cerebral zygomycosis in alloxan-diabetic rabbits: variation in virulence among zygomycetes. | we investigated the potential of 33 different zygomycete isolates to cause cerebral disease following the intranasal instillation of their spores into ketotic rabbits with alloxan induced diabetes. the isolates represented six thermotolerant species of rhizopus (r. arrhizus, r. chinensis, r. microsporus, r. oligosporus, r. oryzae, and r. rhizopodiformis), absidia corymbifera, cunninghamella bertholletiae, and rhizomucor pusillus. all 13 isolates of the thermotolerant rhizopus species proved to b ... | 1981 | 6798698 |
| photo-oxidation of a histidyl residue of milk-clotting acid protease, mucor rennin. | mucor rennin, a milk-clotting acid protease produced by a fungus mucor pusillus, was inactivated by photo-oxidation mediated by methylene blue according to first order kinetics. the ph profile of the inactivation rate showed that a dissociating group with a pk value of 7.6 was involved in the inactivation. addition of pepstatin a, an inhibitor specific for acid proteases, caused a marked alkaline shift of the pk value. one of two histidyl residues in the enzyme was destroyed by the photo-oxidati ... | 1982 | 6804452 |
| orbital phycomycosis. | 3 cases with rhinoorbital phycomycosis and a 4th case, in which it was considered retrospectively, are presented in hematological patients. 2 of the 4 patients survived. in the first 3 cases, which appeared in a period of 2 months in the hematology unit, an epidemiological study was made, showing that the cause of the infections was contamination of the air-conditioning systems of this unit by numerous phycomycetes and mucor pusillus. | 1981 | 6943476 |
| murine model of pulmonary mucormycosis in cortisone-treated mice. | intranasal inoculation of rhizomucor pusillus sporangiospores into cortisone-treated mice produced pulmonary and disseminated mucormycosis (phycomycosis). evidence for infection in cortisone treated mice was obtained by recovery of rh. pusillus from homogenates of tissue. confirmation of infection was shown histologically. the 50% infectious dose was 2.4 x 10(2) colony forming units for lung infections and 2.7 x 10(5) colony forming units for brain infections. no evidence of sporangiospore germi ... | 1982 | 7135144 |
| thermal resistance and viability of asexual spores of thermophilic fungi from composts. | the growth-temperature relations and thermal tolerance of the asexual spores of thermophilic fungi commonly found in composting plant materials were determined. the optimum temperature range for germination of the spores was 40 degrees - 50 degrees c. the spores of humicola lanuginosa (griffon and maublanc) bunce were able to survive 60 min exposure to 68 degrees c while those of mucor pusillus cooney and emerson survived only 5 min exposure to 68 degrees c. a determination of the thermal death ... | 1980 | 7193384 |
| nutritional physiology of pathogenic species of thermophilic mucor. | some aspects of the nutritional physiology of zoopathogenic mucor pusillus lindt and mucor miehei cooney & emerson were studied at 37 degrees c. only asexual spores (sporangiospores) were produced on all the media used. a ph range of 5.0-6.0 was found to be best for the growth and sporulation of both species. the fungi were able to utilize various sources of carbon and nitrogen tested except l-sorbose and dl-tryptophan on which no growth was recorded. of the carbon sources, the best results were ... | 1981 | 7292206 |
| fungi from interior organs of free-living small mammals in czechoslovakia and yugoslavia. | a total of 308 fungi was isolated from interior organs (lungs, spleen, liver) of 529 small mammals belonging to 21 species, 7 families and 3 orders (insectivora, chiroptera, rodentia), some of these being potentially pathogenic to vertebrates (e.g. aspergillus flavus, a. fumigatus, geotrichum candidum, mucor pusillus, rhizopus arrhizus). in one vole (microtus arvalis) captured in south moravia, adiaspiromycosis (emmonsia crescens) was demonstrated. comparison of mycoflora of hair and that of int ... | 1980 | 7419129 |
| extracellular amylase activities of rhizomucor pusillus and humicola lanuginosa at initial stages of growth. | among thermophilic fungi, rhizomucor pusillus and humicola lanuginosa have been reported to be among the most prolific producers of amylase, an apparently heat stable enzyme vital to the incorporation of carbon from macromolecular sources such as starch. yet the highest levels of extracellular amylase in starch-yeast cultures of these fungi were measured after most of the growth had occurred; pre-growth levels appeared to be very small. since these low levels are the significant ones for growth, ... | 1994 | 7537865 |
| a comparison of infrared spectra of proteins in solution and crystalline forms. | fourier transform infrared spectroscopy has been used to compare the structure of a range of proteins in solution and in the form of single crystals. an infrared microscope was used to record the spectra of single crystals of the proteins. the proteins studied in this way were hen egg white lysozyme, bovine pancreatic ribonuclease a, bovine gamma-ii crystallin, human serum amyloid p component, endothia parasitica pepsin and mucor pusillus pepsin. the amide i and amide ii bands in the ftir spectr ... | 1995 | 7748892 |
| zygospores: the last word in identification of rare or atypical zygomycetes isolated from clinical specimens. | three zygomycetes (order mucorales), isolated from clinical specimens, whose generic or species identification were uncertain, were definitively identified on the basis of the production of zygospores resulting from mating studies. these three isolates were identified as mucor circinelloides f. circinelloides, rhizomucor pusillus, and absidia corymbifera. the production of true zygospores, the "last word" in zygomycete taxonomy, should be considered as a diagnostic tool for the definitive identi ... | 1995 | 7751398 |
| mutation of a fungal aspartic proteinase, mucor pusillus rennin, to decrease thermostability for use as a milk coagulant. | mutagenesis of a fungus mucor pusillus, a producer of an aspartic proteinase named mucor pusillus rennin (mpr), was performed to obtain the mutated enzymes with decreased thermostability, which is desirable for practical use of the enzyme as a milk coagulant for cheese manufacturing. a fungal mutant strain which produced the mutant enzyme with distinctly reduced thermostability was isolated. two different mutant alleles of the mpr gene, one with a single amino acid exchange of ala101 for thr and ... | 1994 | 7764448 |
| secretion by saccharomyces cerevisiae of rat apolipoprotein e as a fusion to mucor rennin. | as the first step for production of rat apolipoprotein e (rapoe) in saccharomyces cerevisiae, the rapoe cdna was cloned and its nucleotide sequence was determined. when the intact rapoe gene including the presequence-encoding region was expressed under the control of the yeast gal7 promoter, no protein immunoreactive with anti-rapoe antibody was detected either in the culture medium or inside the cells. for the purpose of the extracellular production of rapoe, three fusion genes were constructed ... | 1995 | 7766086 |
| immunohistochemical identification of aetiological agents of systemic bovine zygomycosis. | the reactivity of a panel of rabbit hyperimmune antisera raised against somatic antigens of three zygomycetes, absidia corymbifera, rhizopus oryzae and rhizomucor (mucor) pusillus was assessed by crossed immunoelectrophoresis (xie) and indirect immunofluorescence (iif) staining of experimentally infected murine tissues. despite a widespread cross-reactivity of the neat antisera, heterologous absorption rendered antisera monospecific as assessed by xie. heterologous absorption also rendered the a ... | 1994 | 8040374 |
| a mucor pusillus mutant defective in asparagine-linked glycosylation. | a mucor pusillus mutant defective in asparagine-linked glycosylation was found in our stock cultures. this mutant, designated 1116, secreted aspartic proteinase (mpp) in a less-glycosylated form than that secreted by the wild-type strain. analysis of enzyme susceptibility, lectin binding, and carbohydrate composition indicated that this mutant secreted three glycoforms of mpps, one of which contained no carbohydrate; the other two had truncated asparagine-linked oligosaccharide chains such as ma ... | 1994 | 8169212 |
| characterization of an aspartic proteinase of mucor pusillus expressed in aspergillus oryzae. | the aspartic proteinase (mpp) gene from the zygomycete fungus mucor pusillus was introduced into an ascomycete fungus, aspergillus oryzae, by protoplast transformation using the nitrate reductase (niad) gene as the selective marker. southern blot analysis indicated that the mpp gene was integrated into the resident niad locus at a copy number of 1-2. mpp secreted by the recombinant a. oryzae was correctly processed but was more highly glycosylated than that produced in the original m. pusillus s ... | 1993 | 8246885 |
| survey of mycoflora and mycotoxins in egyptian soybean seeds. | after four months in commercial storage, 100 soybean samples from different places of egyptian governorates were assayed for filamentous fungal growth at two incubation temperatures (28 and 45 degrees c). 73 species and 8 varieties belonging to 32 genera were isolated by the dilution plate method. at 28 degrees c, the common species were aspergillus flavus, a. fumigatus, a. niger and a. alutaceus, followed by a. terreus, penicillium chrysogenum, p. citrinum, mucor hiemalis, m. racemosus, emerice ... | 1993 | 8271157 |
| mycoflora and natural occurrence of mycotoxins in tobacco from cigarettes in egypt. | forty-two species and 4 varieties belonging to 21 genera were collected from 40 tobacco samples on glucose- and cellulose-czapek's agar at 28 degrees c and 45 degrees c. the most common mesophiles (at 28 degrees c) in tobacco on the two types of media were: aspergillus flavus, a. flavus var. columnaris, a. fumigatus, a. niger, penicillium chrysogenum and p. funiculosum. two samples were heavily contaminated with members of fusarium (f. moniliforme, f. oxysporum, f. solani). some fungi were encou ... | 1993 | 8368025 |
| x-ray analyses of aspartic proteinases. v. structure and refinement at 2.0 a resolution of the aspartic proteinase from mucor pusillus. | the structure of mucor pusillus pepsin (ec 3.4.23.6), the aspartic proteinase from mucor pusillus, has been refined to a crystallographic r-factor of 16.2% at 2.0 a resolution. the positions of 2638 protein atoms, 221 solvent atoms and a sulphate ion have been determined with an estimated root-mean-square (r.m.s.) error of 0.15 to 0.20 a. in the final model, the r.m.s. deviation from ideality for bond distances is 0.022 a, and for angle distances it is 0.050 a. comparison of the overall three-di ... | 1993 | 8450540 |
| mycoflora and mycotoxin of hazelnut (corylus avellana l.) and walnut (juglans regia l.) seeds in egypt. | fifty-one species and 3 varieties appertaining to 20 genera were collected from 20 samples of each of hazelnut and walnut seeds on glucose- and 40% (w/v) sucrose-czapek's agar at 25 degrees c and 45 degrees c with the most common mesophiles were aspergillus flavus, a. fumigatus, a. niger, cladosporium cladosporioides, c. herbarum, penicillium chrysogenum, p. citrinum and p. oxalicum. fusarium (represented by f. equiseti, f. moniliforme and f. oxysporum) was recovered from walnut seeds in moderat ... | 1993 | 8480455 |
| infection due to rhizomucor pusillus: report of four cases in patients with leukemia and review. | rhizomucor pusillus, a thermophilic fungus of the order mucorales, is a rare cause of human infection. a search of the literature has produced only seven reports describing nine cases of infection caused by this organism. recently, over a period of 17 months, four cases of r. pusillus infection in patients with leukemia were diagnosed: a cluster of three cases in a montreal hospital and one isolated case from quebec city. all four cases were proven both by histopathologic examination and by cult ... | 1993 | 8507755 |
| tyrosine 75 on the flap contributes to enhance catalytic efficiency of a fungal aspartic proteinase, mucor pusillus pepsin. | | 1995 | 8540364 |
| site-directed mutagenesis revealed role of subsite residues of mucor pusillus pepsin in catalytic function. | | 1995 | 8540372 |
| integrative transformation of the zygomycete rhizomucor pusillus by homologous recombination. | for development of a homologous transformation system for the zygomycete fungus, rhizomucor pusillus, the isopropylmalate isomerase (leua) gene was cloned from r. pusillus ifo 4578 by the dna-probing method with the leua sequence of mucor circinelloides as probe. the nucleotide sequence revealed that leua of r. pusillus encoded a 755-amino-acid protein of 82.5 kda with no intron. the leua gene on puc19 (plasmid prpleu10) was introduced by polyethyleneglycol-assisted transformation into protoplas ... | 1996 | 8766701 |
| [disseminated aspergillosis and mucormycosis. a case report]. | this paper reports a simultaneous pulmonary infection due to aspergillus fumigatus and rhizomucor pusillus which evolved to disseminated aspergillosis and mucormycosis in a leukaemic and neutropenic 40-year-old woman. both fungi were cultured ante mortem from bronchial secretion. although high doses of intravenous amphotericin b were administered, the course of the infection was fatal. at autopsy, aspergilli were demonstrated by histology in lungs and brain, and zygomycetes were found to have in ... | 1996 | 8767273 |
| secretion of a variant of human single-chain urokinase-type plasminogen activator without an n-glycosylation site in the methylotrophic yeast, pichia pastoris and characterization of the secreted product. | human single-chain urokinase-type plasminogen activator without an n-glycosylation site (scu-pa-q302) was produced in the methylotrophic yeast, pichia pastoris using the shortened prepeptide sequence of a fungal aspartic proteinase, mucor pusillus rennin (mpr). the level of urokinase-type plasminogen activator (u-pa) immunoreactive material in ypm medium was 0.47 mg/l; however, most of the secreted product had been processed to smaller polypeptides. the n-terminal amino acid sequence of major sp ... | 1996 | 8771709 |
| immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies. | murine monoclonal antibodies (mabs) against water-soluble somatic antigens (wssa) and the wall fraction (wf) from rhizopus arrhizus (rhizopus oryzae) were produced in vitro by fusion of splenocytes from immunized balb/c mice with mouse myeloma x63-ag 8.653 cells. supernatants reacting only with homologous antigens in an enzyme-linked immunosorbent assay were subsequently screened for reactivity with homologous fungi in immunohistochemical techniques. all four mabs raised against the wf of a. arr ... | 1996 | 8801711 |
| involvement of a residue at position 75 in the catalytic mechanism of a fungal aspartic proteinase, rhizomucor pusillus pepsin. replacement of tyrosine 75 on the flap by asparagine enhances catalytic efficiency. | residue 75 on the flap, a beta hairpin loop that partially covers the active site cleft, is tyrosine in most members of the aspartic proteinase family. site-directed mutagenesis was carried out to investigate the functional role of this residue in rhizomucor pusillus pepsin, an aspartic proteinase with high milk-clotting activity produced by the fungus rhizomucor pusillus. a set of mutated enzymes with replacement of the amino acid at position 75 by 17 other amino acid residues except for his an ... | 1996 | 8931126 |
| [zygomycosis: review of 16 cases]. | zygomycosis (mucormycosis or phycomycosis) is the infection caused by zygomycetes (non partitioned mycelial fungi, usually saprophytes). all cases of zygomycosis diagnosed in the hospital 12 de octubre in madrid, spain, from 1976 to 1994 were reviewed. | 1996 | 9044638 |
| site-directed mutagenesis of conserved trp39 in rhizomucor pusillus pepsin: possible role of trp39 in maintaining tyr75 in the correct orientation for maximizing catalytic activity. | replacement of trp39 of rhizomucor pusillus pepsin (rmpp) by asn or cys resulted in a marked decrease in the milk-clotting and proteolytic activities. kinetic analysis with chromogenic synthetic oligopeptides as substrates revealed that the mutations caused marked changes in the kcat value, but only slight changes in the km value. similar enzymatic properties were observed in mutants of tyr75, which was shown to have a role in enhancing the catalytic activity. both tyr75asn and trp39asn mutants ... | 1997 | 9058201 |
| incidence and detection of thermotolerant and thermophilic fungi from maize with particular reference to thermoascus species. | a number of thermotolerant and thermophilic fungi were isolated from shipments of food-aid grain, and from large bag stacks of maize stored in sub-saharan africa. thermotolerant fungi included aspergillus candidus, a. fumigatus, a. flavus and paecilomyces varioti; thermophilic fungi included thermomyces lanuginosus, rhizomucor pusillus, thermoascus aurantiacus and t. crustaceous. temperature profiles for thermoascus spp. indicated that isolates of t. aurantiacus grew up to 60 degrees c, and t. c ... | 1997 | 9105921 |
| crystal structure of the aspartic proteinase from rhizomucor miehei at 2.15 a resolution. | the crystal structure of the aspartic proteinase from rhizomucor miehei (rmp, ec 3. 4. 23. 23) has been refined to 2.15 a resolution to a crystallographic r-value of 0.215 and an rfree of 0.281. the root-mean-square (r.m.s.) error for the atomic coordinates estimated from a luzzati plot is 0.2 a. the r.m.s. deviations for the bond distances and bond angles from ideality are 0.01 a and 1.7 degrees, respectively. rmp contains two domains that consist predominantly of beta-sheets. a large substrate ... | 1997 | 9159482 |
| structure of asparagine-linked oligosaccharides of an aspartic proteinase from the zygomycete fungus rhizomucor pusillus. | the zygomycete fungus rhizomucor pusillus (previously called mucor pusillus) secretes an aspartic proteinase containing two asparagine-linked, high-mannose type oligosaccharide chains at asn79 and asn188. for structural elucidation of the carbohydrate moieties, the protein was divided into two portions, an n-terminal portion containing asn79 and a c-terminal portion containing asn188, by a specific autocatalytic cleavage under alkaline conditions. each of the asparagine-linked oligosaccharides w ... | 1998 | 9611811 |
| differentiation of rhizomucor species by carbon source utilization and isoenzyme analysis. | nineteen rhizomucor miehei and rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. five isoenzyme systems also proved useful for the determination of markers of distinctive value at a species level. data were used to obtain information about the genetic polymorphism of these species: a h ... | 1998 | 9650990 |
| variation in n-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast pichia pastoris. | we report the characterization of n-linked oligosaccharides on six foreign glycoproteins secreted from the methylotrophic yeast pichia pastoris. these proteins included: a bacterial enzyme, bacillus licheniformis alpha-amylase; three fungal enzymes, saccharomyces cerevisiae invertase, penicillium minioluteum dextranase, and mucor pusillus aspartic protease; and two higher eukaryotic proteins, boophilus microplus (tick) gut antigen and bovine enterokinase catalytic subunit. the carbohydrates on t ... | 1998 | 9790882 |
| characterization of alg2 encoding a mannosyltransferase in the zygomycete fungus rhizomucor pusillus. | alg2 of saccharomyces cerevisiae encodes the glycosyltransferase that mannosylates man2glcnac2-dolichol diphosphate (pp-dol) and man1glcnac2-pp-dol to form man3glcnac2-pp-dol. the genomic dna and cdna encoding an alg2 homologue were cloned from the zygomycete fungus, rhizomucor pusillus, and their nucleotide sequences were determined. the cloned cdna under the control of the yeast gal1 promoter complemented the temperature-sensitive (ts) growth of the alg2-1 mutant of s. cerevisiae, indicating t ... | 1998 | 9795208 |
| rhizomucor pusillus mucormycosis combined with chlamydiosis in an african grey parrot (psittacus erithacus erithacus). | this paper describes a case of mucormycosis combined with chlamydiosis in an african grey parrot (psittacus erithacus erithacus). the clinical signs included diarrhoea, an unsteady gait and a twisted neck. smears of the spleen, liver and contents of the cloaca stained strongly positive for chlamydia species. histologically, hyphae typical of zygomycetes were observed invading through the walls of blood vessels of the spleen. rhizomucor pusillus was isolated as a pure culture from the intestines, ... | 1998 | 9823607 |
| [fungal flora in chicken stalls and its etiopathogenic importance for humans and animals]. | in order to find out the mycoflora prevailing in chicken pens, and to appreciate the health hazards for employees (incl. veterinarians) and animals, twenty litter samples and 6 sedimented dust trials were analysed mycologically. the following results were found: bedding and dust samples all contained between 3.57 and 1.30 x 10(7) c.f.u./g dw. the commonest fungus is a. fumigatus with 3.41 x 10(4) - 1.30 x 10(7) c.f.u./g dw in bedding and 2.70 x 10(5) - 3.30 x 10(6) c.f.u./g dw in sedimented dust ... | 1999 | 10209909 |
| genetic transformation of a rhizomucor pusillus mutant defective in asparagine-linked glycosylation: production of a milk-clotting enzyme in a less-glycosylated form. | rhizomucor pusillus 1116r3 has a defect in alg2 encoding a mannosyltransferase in the asparagine (n)-linked oligosaccharide biosynthetic pathway and produces proteins in less-glycosylated forms. for development of a genetic transformation system for this zygomycete, an uracil auxotroph (mutant 1116u17) as the host strain was derived by ultraviolet (uv) mutagenesis as 5-fluoroorotic acid-resistant colonies and the orotidine-5'-monophosphate (omp) decarboxylase (pyr4) gene as a selection marker wa ... | 1999 | 10531653 |
| characterization of an alg2 mutant of the zygomycete fungus rhizomucor pusillus. | the zygomycete fungus rhizomucor pusillus secretes an aspartic proteinase (mpp) that contains asparagine ( n )-linked oligosaccharides at two sites. mutant strain 1116 defective in n -glycosylation secretes mpp with truncated oligo-saccharide chains. lipid-linked oligosaccharides in mutant 1116 were labeled with [6-(3)h]glucosamine and [2-(3)h]mannose, prepared by cycles of solvent extraction, and analyzed by gel filtration chromatography on a bio-gel p-4 column after mild acid-hydrolysis. mutan ... | 1999 | 10561453 |
| phylogeny and pcr identification of clinically important zygomycetes based on nuclear ribosomal-dna sequence data. | a molecular database for all clinically important zygomycetes was constructed from nucleotide sequences from the nuclear small-subunit (18s) ribosomal dna and domains d1 and d2 of the nuclear large-subunit (28s) ribosomal dna. parsimony analysis of the aligned 18s and 28s dna sequences was used to investigate phylogenetic relationships among 42 isolates representing species of zygomycetes reported to cause infections in humans and other animals, together with commonly cultured contaminants, with ... | 1999 | 10565914 |
| characterization of mucor pusillus rennin expressed in pichia pastoris: enzymic, spectroscopic and calorimetric studies. | the aspartic proteinase gene of mucor pusillus rennin expressed in pichia pastoris was characterized in terms of structural and conformational stability induced by temperature. this enzyme is 12% glycosylated, with a similar specific activity to the native fungal enzyme. the secondary structure determined by cd is mainly due to beta-sheet structures with an important contribution of aromatic components. the calorimetric studies were carried out in the temperature range in which the enzyme is mos ... | 2000 | 10744950 |
| intraspecific variation in two species of rhizomucor assessed by random amplified polymorphic dna analysis. | twenty-three rhizomucor isolates were characterized by random amplified polymorphic dna-pcr (rapd-pcr) with 10-bp oligonucleotide primers. these data were used for numerical analyses to obtain information on the intraspecific genetic polymorphism of rhizomucor species. the genetic variability in rhizomucor pusillus and rhizomucor miehei isolates was found to differ; the latter revealed less intraspecific polymorphism. the different levels of genotypic diversity suggest a correlation with the dif ... | 2000 | 10986673 |