alliinase [s-alk(en)yl-l-cysteine sulfoxide lyase] from allium tuberosum (chinese chive)--purification, localization, cdna cloning and heterologous functional expression.alliinase [s-alk(en)yl-l-cysteine sulfoxide lyase], a pyridoxal-phosphate-(pxy-p)-dependent enzyme, is responsible for the degradative conversion of s-alk(en)yl-l-cysteine sulfoxide to volatile odorous sulfur-containing metabolites in allium plants. we have purified alliinase from shoots of allium tuberosum (chinese chive) to apparent homogeneity by sds/polyacrylamide gel electrophoresis. a cdna clone encoding alliinase was isolated from a cdna library constructed from whole plants of a. tuberos ...19989799098
a robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive allium tuberosum.from the inner shoots of the chive allium tuberosum, a single-chained protein with a molecular weight of 36 kda and an n-terminal sequence manifesting resemblance to chitinases but lacking in cysteine residues characteristic of a cysteine-rich domain present in chitinases of other allium species, was purified. the isolation procedure entailed affinity chromatography on affi-gel blue gel, ion-exchange chromatography on deae-cellulose and mono s, and gel filtration on superdex 75. the protein was ...200011112420
antimicrobial effect of extracts from chinese chive, cinnamon, and corni fructus.extracts were prepared from chinese chive (allium tuberosum), cinnamon (cinnamomum cassia), and corni fructus (cornus officinalis) and used to evaluate their antimicrobial activity on common foodborne microorganisms, alone and in combination. the mixed extract, consisting of three extracts in equal volumes, showed an entire antimicrobial spectrum and had excellent stability to heat, ph, and storage. the mixed extract exhibited better inhibition on growth of escherichia coli than potassium sorbat ...200111170575
antibacterial activity of s-methyl methanethiosulfinate and s-methyl 2-propene-1-thiosulfinate from chinese chive toward escherichia coli o157:h7.s-methyl methanethiosufinate (1) and s-methyl 2-propene-1-thiosulfinate (2) were easily seperated from chinese chive (allium tuberosum l.) using simple column chromatography. both compounds showed significant antibacterial activities against e. coli o-157:h7 including spoilage microorganism in food. structural assignment was based on mass and nmr-spectroscopic methods.200111388483
a pilot study on anticancer activities of chinese investigate the anticancer activities of chinese leek (allium tuberosum rottler; [cl]).200111719943
a monomeric mannose-binding lectin from inner shoots of the edible chive (allium tuberosum).a mannose-binding lectin was isolated from the inner shoots of the chive allium tuberosum. the procedure involved aqueous extraction, (nh4)2so4 precipitation, dialysis to remove (nh4)2so4, affinity chromatography on mannose-agarose, ion exchange chromatography on sp-sepharose, gel filtration on superdex 75, and ion exchange chromatography on mono s. lectin activity was adsorbed on mannose-agarose, sp-sepharose, and mono s. the lectin demonstrated a molecular weight of 13 kda in sodium dodecyl su ...200111732687
isolation and characterization of a bioactive mannose-binding protein from the chinese chive allium tuberosum.a mannose-binding protein was isolated from two different cultivars of the chinese chive allium tuberosum by extraction with 0.2 m nacl, ammonium sulfate precipitation, and affinity chromatography on mannose agarose and fetuin agarose. it exhibited hemagglutinating activity toward rabbit erythrocytes. the lectin (agglutinin) was adsorbed on the mannose-agarose column, but not on the fetuin-agarose column. this a. tuberosum lectin (atl) is unglycosylated, and not sialic acid binding. lectins isol ...200211829630
tuberoside m, a new cytotoxic spirostanol saponin from the seeds of allium tuberosum.tuberoside m (1), isolated from the seeds of allium tuberosum, shows a significant inhibitory effect on the growth of the human promyelocytic leukemia cell line (hl-60) with ic50 value of 6.8 microg/ml. on the basis of spectral data and chemical reaction, its structure was established as (25s)-5beta-spirostane-1beta,3beta-diol 3-o-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside.200211995668
ascalin, a new anti-fungal peptide with human immunodeficiency virus type 1 reverse transcriptase-inhibiting activity from shallot isolation procedure comprising ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose and gel filtration on superdex 75 was used to isolate an anti-fungal peptide from the bulbs of the shallot allium ascalonicum. the peptide demonstrated a molecular weight of 9.5kda, and possessed an n-terminal sequence yqcgqgg somewhat similar to chitinases from other allium species which are however much larger in molecular we ...200212126728
heinz body hemolytic anemia with eccentrocytosis from ingestion of chinese chive (allium tuberosum) and garlic (allium sativum) in a dog.a 4-year-old, intact male miniature schnauzer was presented with anorexia. the dog had ingested some chinese steamed dumplings 2 days before, which contained chinese chive (allium tuberosum) and garlic (allium sativum). hematological examinations revealed severe heinz body hemolytic anemia with eccentrocytosis and an increased concentration of methemoglobin, which was thought to result from oxidative damage to erythrocytes by constituents in these allium plants. in this case, eccentrocytosis was ...200515634869
molecular and biochemical characterisation of a serine acetyltransferase of onion, allium cepa (l.).we have previously cloned a cdna, designated sat1, corresponding to a gene coding for a serine acetyltransferase (sat) from onion (allium cepa l.). the sat1 locus was mapped to chromosome 7 of onion using a single-stranded conformation polymorphism (sscp) in the 3' utr of the gene. northern analysis has demonstrated that expression of the sat1 gene is induced in leaf tissue in response to low s-supply. phylogenetic analysis has placed sat1 in a strongly supported group (100% bootstrap) that comp ...200515949827
allium vegetable diet can reduce the exercise-induced oxidative stress but does not alter plasma cholesterol profile in rats.this study investigated the effect of allium vegetable intake on the antioxidative activity and on the plasma cholesterol profile during exercise in rats. ninety rats were fed either a control diet or a diet with added allium sativum (as), allium cepa (ac), allium fistulosum (af), or allium tuberosum (at) for 4 weeks and were then subdivided into three groups: before exercise (be), during exercise (de), and after exercise (ae). the de group was exercised on a treadmill for 1 h immediately before ...200616391468
cytotoxic and antitumor activities of thiosulfinates from allium tuberosum this study we isolated crude thiosulfinates from allium tuberosum l. using ch 2cl 2 and then with silica gel column chromatography purified s-methyl methanthiosulfinate and s-methyl 2-propene-1-thiosulfinate from the crude thiosulfinates. subsequently, in vitro cytotoxicities against human cancer cells and in vivo antitumor activities of the thiosulfinates were investigated. their cytotoxicities were strong in human cancer cells, in the order of s-methyl 2-propene-1-thiosulfinate, crude thios ...200717711341
aphrodisiac properties of allium tuberosum seeds the present study, we examined the effect of allium tuberosum seeds extract upon the expression of male rat sexual behavior, in order to know whether allium tuberosum seeds extract possess aphrodisiac property.200919429330
a new fibrinolytic enzyme (55 kda) from allium tuberosum: purification, characterization, and comparison.chives have been used both as food and as medicine. previously, two fibrinolytic enzymes, atfe-i (90 kda) and atfe-ii (55 kda), were identified in chives (allium tuberosum), a perennial herb. in the present work, atfe-ii was purified by ion-exchange chromatography followed by gel filtration. in addition, the enzyme properties of atfe-i and atfe-ii were compared. the molecular mass and isoelectric point (pi value) of atfe-ii were 55 kda and pi 4.0, respectively, as revealed using one- or two-dime ...201020954802
action of coriandrum sativum l. essential oil upon oral candida albicans biofilm formation.the efficacy of extracts and essential oils from allium tuberosum, coriandrum sativum, cymbopogon martini, cymbopogon winterianus, and santolina chamaecyparissus was evaluated against candida spp. isolates from the oral cavity of patients with periodontal disease. the most active oil was fractionated and tested against c. albicans biofilm formation. the oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. the minimal inhibitory concentratio ...201121660258
occurrence of sclerotium rot in allium tuberosum caused by sclerotium rolfsii in this study, we characterized sporadically occurring sclerotium rot caused by sclerotium rolfsii in chinese chive (allium tuberosum roth.) in farm fields in sacheon, korea. the initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the scaly stem and leaves. the sclerotia were globoid, 1~3 mm, and white to brown. the optimum temperature for ...201122783108
control of fusarium wilt in banana with chinese leek.the inhibitory effects of chinese leek(allium tuberosum) on fusarium oxysporum f. sp. cubense (foc) and on fusarium wilt incidence were studied in order to identify a potential efficient way to control the disease. adopting the rotation system of chinese leek-banana reduced the fusarium wilt incidence and disease severity index by 88 %-97 % and 91 %-96 %, respectively, improved the crop value by 36 %-86 %, in an area heavily infested by foc between 2007 and 2009. as a result of inoculation in th ...201223144534
transcriptome analysis and discovery of genes relevant to development in bradysia odoriphaga at three developmental stages.bradysia odoriphaga (diptera: sciaridae) is the most important pest of chinese chive (allium tuberosum) in asia; however, the molecular genetics are poorly understood. to explore the molecular biological mechanism of development, illumina sequencing and de novo assembly were performed in the third-instar, fourth-instar, and pupal b. odoriphaga. the study resulted in 16.2 gb of clean data and 47,578 unigenes (≥125 bp) contained in 7,632,430 contigs, 46.21% of which were annotated from non-redunda ...201626891450
laboratory evaluation of acute toxicity of the essential oil of allium tuberosum leaves and its selected major constituents against apolygus lucorum (hemiptera: miridae).the aim of this research was to evaluate acute toxicity of the essential oil of leaves of chinese chives, allium tuberosum rottler ex spreng (asparagales: alliaceae) and its major constituents against apolygus lucorum meyer-dür (hemiptera: miridae). the essential oil of a. tuberosum leaves was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography-mass spectrometry. the major constituents of the oil were sulfur-containing compounds, including allyl methyl trisulf ...201526254289
antifungal effect of allium tuberosum, cinnamomum cassia, and pogostemon cablin essential oils and their components against population of aspergillus species.antifungal activity of allium tuberosum (at), cinnamomum cassia (cc), and pogostemon cablin (patchouli, p) essential oils against aspergillus flavus strains 3.2758 and 3.4408 and aspergillus oryzae was tested at 2 water activity levels (aw : 0.95 and 0.98). main components of tested essential oils were: allyl trisulfide 40.05% (at), cinnamaldehyde 87.23% (cc), and patchouli alcohol 44.52% (p). the minimal inhibitory concentration of the plant essential oils against a. flavus strains 3.2758 and 3 ...201323647469
chemical composition, antibacterial and antioxidant activities of six essentials oils from the alliaceae family.six essential oils (eos) from the alliaceae family, namely garlic (allium sativum), onion (allium cepa), leek (allium porrum), chinese chive (allium tuberosum), shallot (allium ascalonicum) and chive (allium schoenoprasum) were characterized by gc and gc-ms and evaluated for their functional food properties. antibacterial properties were tested on five food-borne pathogens: two gram-positive staphylococcus aureus (atcc 25923), listeria monocytogenes (atcc 19115) and three gram-negative salmonell ...201425470273
effects of combined treatments of electron-beam irradiation and addition of leek (allium tuberosum) extract on reduction of pathogens in pork jerky.this study investigated the combined effect of electron-beam irradiation and addition of leek (allium tuberosum r.) extract on pork jerky inoculated with selected foodborne pathogens. prepared pork jerky samples (control and samples with 1.0% leek extract) were inoculated with pathogens and subsequently irradiated at 0, 0.5, 1, 2, 3, and 4 kgy doses. in comparison with the control, samples with 1.0% leek extract showed significant reduction in the numbers of escherichia coli, listeria monocytoge ...201223199493
control of panama disease of banana by rotating and intercropping with chinese chive (allium tuberosum rottler): role of plant volatiles.intercropping and rotating banana (musa spp.) with chinese chive (allium tuberosum rottler) has been used as an effective method to control panama disease (fusarium wilt) of banana in south china. however, the underlying mechanism is unknown. in this study, we used aqueous leachates and volatiles from chinese chive to evaluate their antimicrobial activity on fusarium oxysporum f. sp. cubense race 4 (foc), the causal agent of panama disease in banana, and identified the antifungal compounds. both ...201323355016
selection of functional lactic acid bacteria as starter cultures for the fermentation of korean leek (allium tuberosum rottler ex sprengel.).the purpose of this research was to find safe and suitable starter cultures for the fermentation of korean leek (allium tuberosum rottler), also known as garlic chives or oriental garlic. this traditional herb has several functional properties and a strong flavour; its leaves are used as food material. eighteen strains of lactic acid bacteria (lab) were isolated from well-fermented leek kimchi. controlled fermentation of the leek leaves was conducted with 2 strains (weissella confusa lk4 and lac ...201425279760
laboratory evaluation of larvicidal activity of the essential oil of allium tuberosum roots and its selected major constituent compounds against aedes albopictus (diptera: culicidae).the aim of this research was to evaluate larvicidal activity of the essential oil of allium tuberosum rottler ex sprengle roots and its constituents against larval mosquitoes (aedes albopictus skuse). essential oil of a. tuberosum was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography-mass spectrometry. the major constituents of the oil were found to be sulfur-containing compounds, including allyl methyl trisulfide (35.19%), diallyl disulfide (28.31%), dially ...201526334818
synergistic effects of electron-beam irradiation and leek extract on the quality of pork jerky during ambient investigate the synergistic effect(s) of electron-beam (eb) irradiation and leek (allium tuberosum rottler) extract on the quality of pork jerky during ambient storage, we irradiated prepared pork jerky samples (control and samples with 0.5% and 1.0% leek extract) with eb technology at doses of 0, 1, 2, and 4 kgy, stored them for 2 months at 25°c, and analyzed them. water activity was 0.73 to 0.77 in non-irradiated samples, and no significant difference in the water activity was observed betw ...201325049828
applications of direct analysis in real time mass spectrometry (dart-ms) in allium chemistry. 2-propenesulfenic and 2-propenesulfinic acids, diallyl trisulfane s-oxide, and other reactive sulfur compounds from crushed garlic and other alliums.through the use of direct analysis in real time mass spectrometry (dart-ms), 2-propenesulfenic acid, an intermediate long postulated as being formed when garlic ( allium sativum ) is crushed, has been detected for the first time and determined by mass spectrometric methods to have a half-life of <1 s at room temperature. two other key intermediates, 2-propenesulfinic acid and diallyl trisulfane s-oxide, have also been detected for the first time in volatiles from crushed garlic, along with allic ...201020225897
survey of nitrate and nitrite contents of vegetables grown in korea.a scientific basis for the evaluation of the risk to public health arising from excessive dietary intake of nitrate in korea is provided. the nitrate () and nitrite () contents of various vegetables (chinese cabbage, radish, lettuce, spinach, soybean sprouts, onion, pumpkin, green onion, cucumber, potato, carrot, garlic, green pepper, cabbage and allium tuberosum roth known as crown daisy) are reported. six hundred samples of 15 vegetables cultivated during different seasons were analysed for ni ...200312888387
isolation and identification of new chemical constituents from chinese chive (allium tuberosum) and toxicological evaluation of raw and cooked chinese chive.chinese chive (jiu cai) is a popular vegetable in china and has a unique flavour and aroma. the molecular basis of the characteristic fragrance and nutritional properties of chinese chive has not been previously identified. sequential extractions in a series of solvents and high-performance liquid chromatography were used to isolate 40 compounds from chinese chive. the compounds were identified based on high-resolution electrospray ionization mass spectra, 1d and 2d nuclear magnetic resonance te ...201728216165
[ecotoxicological effect and soil environmental criteria of the heavy metal chromium(vi)].nowadays, systematic studies about water quality criteria are being carried out in china, but studies on soil environmental criteria are comparatively insufficient. in this study, germination and root growth of 8 terrestrial plants (triticum aestivum, lactuca sativa, cucumis sativus, zea mays, brassica pekinensis, glycine max, allium tuberosum and solanum lycopersicum) and growth inhibition of 1 terrestrial animal (achatina fulica) were used to determine the chronic ecotoxicological effects of c ...201425338393
aphrodisiac activity of polyherbal formulation in experimental models on male investigate the aphrodisiac potential of polyherbal formulations prepared from different parts of tribulus terrestris, curculigo orchioides, allium tuberosum, cucurbita pepo, elephant creeper, mucuna pruriens, and terminalia catappa in albino rats in specified ratio as suspension.201424761115
inhibitory effect of methyl methanethiosulfinate on β-glucuronidase activity.minced or cut-up leaves of chinese chive (allium tuberosum rottler) contain thiosulfinates and their disproportionate reaction products. among these organosulfur compounds, methyl methanethiosulfinate was found to be an uncompetitive inhibitor of β-glucuronidase. approximately 80% of the enzyme activity was inhibited by methyl methanethiosulfinate at 50 µm, the ic50 value being comparable to 3.6 µm.201324200790
[residue of chlorpyrifos and its degradation dynamics in chinese chive (allium tuberosum) plant and soil].this paper studied the residue of chlorpyrifos and its degradation dynamics in chinese chive (allium tuberosum) plant and soil after irrigating different concentrations of this pesticide into a. tuberosum root zone under plastic greenhouse and open-field cultivated conditions. the chlorpyrifos in a. tuberosum plant had a faster degradation rate than that in soil, with an average half-life of the pesticide being 3.41 and 7.40 days in the plant and soil, respectively. under the conditions of plast ...201222586982
influence of biochars on plant uptake and dissipation of two pesticides in an agricultural soil.this study investigated the influence of two types of biochars on the bioavailability of two soil-applied insecticides (chlorpyrifos and fipronil) to chinese chives ( allium tuberosum ) and dissipation of the pesticides in the biochar-amended soils. the biochars (bc450 and bc850) prepared from the burning of cotton ( gossypium spp.) straw chips at two different temperatures (450 and 850 degrees c) were thoroughly mixed into a soil to achieve 0, 0.1, 0.5, and 1% by soil dry weight. chinese chives ...201020545346
differential growth response of various crop species to arbuscular mycorrhizal investigate the growth response of various crop species to mycorrhizal inoculation, arbuscular mycorrhizal fungi were applied to glycine max, vigna angularis, senna tora, hordeum vulgare var. hexastichon. zea mays, sorghum bicolor, allium tuberosum, solanum melongena, and capsicum annuum. the biomass of the inoculated crops was measured every two weeks for the 12-week growth period. by measuring biomass, we calculated the mycorrhizal responsiveness of the nine crop species. among the nine cro ...200923983512
[isolation of sperm cells of allium tub rosum roxb].pollen grains of allium tuberosum roxb broke and released their content including generative cells using osmotic shock method. in a medium containing 0.05% cacl2, 0.01% boric acid, 0.01%kh2po4, 15%peg 10% sucrose (710 mosmol/kg h2o) 86% pollen grains germinated and grew out pollen tubes, which broke after transferred into 6% mannitol solution, and released tube content including generative cell. when pollen grains were cultured in the same medium but adding 0.1% casein, a few generative cells di ...200818959007
activation effects of allium tuberosum rottl. on choline ethanolic extract of allium tuberosum rottl. demonstrated potent choline acetytrasferase (chat) activating activity (45%) among 30 screened korean edible plants. the chat activator of a. tuberoum rottl. was purified and identified as ferulic acid [4-hydroxy-3-methoxycinnamic acid] via silica-gel open-column chromatography, hplc, ei-ms, and 1h/13c-nmr. the ferulic acid from a. tuberoum rottl. exhibited chat activity in a dose-dependent manner and showed notoxicity in a cell viability assay (mt ...200717213651
fatty acid composition of the seed oil of allium tuberosum.the fatty acid compositions of seed oil from ten kinds of allium tuberosum (chinese chive, liliaceae) were determined by gas chromatography-mass spectroscopy (gc-ms). the seed oil of allium tuberosum contained much important fatty acids with linoleic (57.0-71.6%) and palmitic (6.6-9.7%), the most abundant unsaturated and saturated fatty acids, respectively. the total unsaturation for the oil was 79.2-93.0%. it is concluded that allium tuberosum oil is of important nutritional value.200515936941
pregnane- and furostane-type oligoglycosides from the seeds of allium tuberosum.two furostane-type steroidal oligoglycosides (1, 2), together with a new pregnane-type oligoglycoside (3), were obtained from the seeds of allium tuberosum rottler. on the basis of spectroscopic analysis, the structures of three new oligoglycosides (1-3) were elucidated as 26-o-beta-d-glucopyranosyl-(25r)-3beta,22xi,26-trihydroxyl-5alpha-furostane 3-o-beta-chacotrioside, 26-o-beta-d-glucopyranosyl-(25s)-3beta,5beta,6alpha,22xi,26-pentahydroxyl-5beta-furostane 3-o-alpha-l-rhamnopyranosyl-(1-->4)- ...200414709884
[studies on chemical constitutents in seeds of allium tuberosum rottl].to study the chemical constituents in the seed of allium tuberosum.200012512450
transfer function analysis of positron-emitting tracer imaging system (petis) data.quantitative analysis of the two-dimensional image data obtained with the positron-emitting tracer imaging system (petis) for plant physiology has been carried out using a transfer function analysis method. while a cut leaf base of chinese chive (allium tuberosum rottler) or a cut stem of soybean (glycine max l.) was immersed in an aqueous solution containing the [18f] f- ion or [13n]no3- ion, tracer images of the leaf of chinese chive and the trifoliate of soybean were recorded with petis. from ...200212150282
volatile constituents of chinese chive (allium tuberosum rottl. ex sprengel) and rakkyo (allium chinense g. don).volatile components were isolated from chinese chive and rakkyo by simultaneous steam distillation-solvent extraction and analyzed by gc and gc-ms. sulfur compounds account for 88 and 94% of the total volatiles in the isolated extract of chinese chive and rakkyo, respectively. in addition to the sulfur compounds commonly reported in the genus allium, 27 novel volatile sulfur-containing components were found in the isolated extracts of both species. among them were a sulfide, disulfides, trisulfi ...200111312859
[research on volatiles of rakkyo (allium chinense g. don) and chinese chive (allium tuberosum rottl. ex sprengel) based on headspace and the molecular recognition of sers].the head space and the molecular recognition of surface enhanced raman scattering (sers) were used to research volatiles of rakkyo and chinese chive. their volatiles sers spectra were obtained using nano-silver colloid as the substrate. then, volatiles sers spectra of rakkyo and chinese chive were compared respectively with the volatiles sers spectra of liquid allyl methyl sulfide, 1-propanethiol, diallyl disulfide and all possible pairings of the three compounds. the results showed that the rep ...201525970899
a comparison of novel entomopathogenic nematode application methods for control of the chive gnat, bradysia odoriphaga (diptera: sciaridae).bradysia odoriphaga yang & zhang (diptera: sciaridae) is the most serious pest of chinese chive, allium tuberosum chemical pesticide application has become a necessary step to control b. odoriphaga after each of the four to six harvests during the growing season. we compared the effects of application type (nematode-infected cadaver, aqueous nematode suspension, and mixture of cadaver and aqueous suspension) and nematode species (heterorhabditis bacteriophora vs. steinernema carpocapsae) on b. o ...201627480971
spirostanol steroids from the roots of allium tuberosum.three new spirostanol saponins named tuberosines a-c (1-3), together with three known ones tuberoside o (4), 25(s)-schidigera-saponin d5 (5), and shatavarin iv (6) were isolated from the roots of allium tuberosum. their structures were established on the basis of extensive spectroscopic analyses. whereas compounds 5 and 6 exhibited potent antibacterial activities against bacillus subtilis (32 μg/ml) and escherichia coli (16 μg/ml), the new saponin 2 showed only moderate antibacterial activities ...201525836597
mechanisms of thiosulfinates from allium tuberosum l.-induced apoptosis in ht-29 human colon cancer cells.this study was performed to elucidate the apoptotic pathways by thiosulfinates, major biologically active components of allium tuberosum l., in ht-29 human colon cancer cells. thiosulfinates significantly induced cell death in dose- and time-dependent manners in ht-29 cells, which is associated with apoptosis. thiosulfinates activated the initiator caspase-8, and -9, and the effector caspase-3. in the present study, thiosulfinates were found to stimulate bid cleavage, indicating that the apoptot ...200919446247
induction of apoptosis by thiosulfinates in primary human prostate cancer cells.thiosulfinates, a substance of allium tuberosum l., is a known folk medicine that has been extensively used in diet to treat diseases. in the present study, we have evaluated the effect of thiosulfinates from allium tumberosum l. on proliferation of metastasis (du145) and primary malignant tumor (rc-58t/h/sa#4)-derived human prostate cancer cells. thiosulfinates decrease viable cell numbers in a dose- and time-dependent manner and induce apoptosis. the apoptosis induced by thiosulfinates is asso ...200818360714
thiosulfinates from allium tuberosum l. induce apoptosis via caspase-dependent and -independent pathways in pc-3 human prostate cancer cells.this study was aimed to evaluate the apoptotic effects of thiosulfinates purified from allium tuberosum l. on pc-3 human prostate cancer cells, and to elucidate detailed apoptosis mechanisms. thiosulfinates significantly decrease viable cell numbers in dose- and time-dependent manners by apoptotic cell death via dna fragmentation, chromatin condensation, and an increased sub-g1 phase. apoptosis induced by thiosulfinates is associated with the activation of initiator caspase-8 and -9, and the eff ...200818024112
[bioassay on oviposition repellency of non-preferable plant extracts against citrus red mite panonychus citri].the oviposition repellency of the alcohol extracts from 50 species of non-preferable plants and azadirachtin against citrus red mite (panonychus citri) was determined using laboratory bioassays. in choice tests, the extracts from 42 of the 50 plant species and 1% azadirachtin (2000 x) significantly reduced oviposition 1d after treatment. the repellency effect of the extracts from the 42 plant species was better than that of azadirachtin. mikania micrantha extract had the best result, with an int ...200415669510
molecular cloning and functional characterization of cdnas encoding cysteine synthase and serine acetyltransferase that may be responsible for high cellular cysteine content in allium tuberosum.the plants belonging to the genus allium are known to accumulate sulfur-containing secondary compounds that are derived from cysteine. here, we report on molecular cloning and functional characterization of two cdnas that encode serine acetyltransferase and cysteine synthase from a. tuberosum (chinese chive). the cdna for serine acetyltransferase encodes an open reading frame of 289 amino acids, of which expression could complement the lacking of cyse gene for endogenous serine acetyltransferase ...200011080593
two new spirostanol saponins from allium tuberosum.two new spirostanol saponins, tuberosides d and e, have been isolated from the seeds of allium tuberosum. on the basis of spectral data and chemical reactions, their structures were established as (25s)-5alpha-spirostane-2alpha,3beta-diol 3-o-alpha-l-rhamnopyranosyl-(1-->2)-o-[alpha-l-rhamnopyranosyl-(1-->4 )]-o-beta-d-glucopyranoside and (25s)-5alpha-spirostan-2alpha, 3beta-diol 3-o-beta-d-glucopyranosyl-(1-->2)-o-[alpha-l-rhamnopyranosyl-(1-->4)] -o-beta-d-glucopyranoside, respectively.199910425134
steroidal oligoglycosides from the seeds of allium tuberosum.three new spirostanol steroidal oligoglycosides, together with a known oligoglycoside, were obtained from the seeds of allium tuberosum after enzymatic hydrolysis of furostanol saponin fraction by beta-glucosidase. on the basis of spectroscopic analysis, the structure of new spirostanol oligoglycosides were elucidated as (25s)-spirost-5-ene-2alpha,3beta-diol 3-o-alpha-l-rhamnopyranosyl-(1-->4)-[alpha-l-rhamnopyranosyl-(1-->2)]-be ta-d-glucopyranoside, (25s)-spirostane-3beta,5beta,6alpha-triol 3- ...200010726858
sphingosine derivatives from the seeds of allium tuberosum.a new ceramide, named tuber-ceramide (2), along with a known cerebroside (1) were isolated from the seeds of allium tuberosum. the structure of tuber-ceramide was determined on the basis of spectral data as n-(2',3'-dihydroxy-tetracosenoyl)-2-amino-1,3,4-trihydroxy octadecane (2). this is the first report of sphingosine derivatives isolated from the genus allium.199911261207
four new steroidal saponins from the seeds of allium tuberosum.four new steroidal saponins, 26-o-beta-d-glucopyranosyl-(25s,20r)-20-o-methyl-5alpha-furost-22(23)-en-2alpha,3beta,20,26-tetraol 3-o-alpha-l-rhamnopyranosyl-(1-->2)-[alpha-l-rhamnopyranosyl-(1-->4)]-beta-d-glucopyranoside (1); 26-o-beta-d-glucopyranosyl-(25s,20r)-5alpha-furost-22(23)-en-2alpha,3beta,20,26-tetraol 3-o-alpha-l-rhamnopyranosyl-(1-->2)-[alpha-l- rhamnopyranosyl-(1-->4)]-beta-d-glucopyranoside (2); 26-o-beta-d-glucopyranosyl-(25s,20s)-5alpha-furost-22(23)-en-2alpha,3beta,20,26-tetrao ...200111312882
a steroidal saponin from the seeds of allium tuberosum.a steroidal saponin, named tuberoside, together with seven known compounds, were isolated from the seeds of allium tuberosum rottl. ex spreng. its structure was established by spectroscopic data, hydrolysis, and comparison with spectral data of known compounds to be (2alpha, 3beta, 5alpha, 25s)-2,3,27-trihydroxyspirostane 3-o-alpha-l-rhamnopyranoyl-(1-->2)-o-[alpha-l-rhamnopyranoyl-(1-->4)]-beta-d-glucopyranoside.200111454347
new spirostanol saponins from chinese chives (allium tuberosum).three new spirostanol saponins have been isolated from the seeds of allium tuberosum. on the basis of acid hydrolysis and comprehensive spectroscopic analysis, their structures were established as tuberoside j, (25r)-5alpha-spirostan-2alpha,3beta,27-triol 3-o-alpha-l-rhamnopyranosyl-(1-->2)-beta-d-glucopyranoside; tuberoside k, (25r)-5alpha-spirostan-2alpha,3beta,27-triol 3-o-alpha-l-rhamnopyranosyl-(1-->2)-[alpha-l-rhamnopyranosyl-(1-->4)]-beta-d-glucopyranoside; and tuberoside l, 27-o-beta-d-g ...200111600021
[concentrations of mercury in ambient air in wastewater irrigated area of tianjin city and its accumulation in leafy vegetables].gaseous hg can evaporate and enter the plants through the stomata of plat leaves, which will cause a serious threat to local food safety and human health. for the risk assessment, this study aimed to characterize atmospheric mercury (hg) as well as its accumulation in 5 leafy vegetables (spinach, edible amaranth, rape, lettuce, allium tuberosum) from sewage-irrigated area of tianjin city. bio-monitoring sites were located in paddy (wastewater irrigation for 30 a), vegetables (wastewater irrigati ...201425639114
two new phenylpropane glycosides from allium tuberosum rottler.a phytochemical investigation of allium tuberosum rottler afforded two new phenylpropane glycosides, named tuberonoid a (1) and b (2), along with four known flavonoids, kaempferol 3-o-β-sophoroside (3), 3-o-β-d-(2-o-feruloyl)-glucosyl-7,4'-di-o-β-d-glucosylkaempferol (4), 3-o-β-sophorosyl-7-o-β-d-(2-o-feruloyl)glucosyl kaempferol (5), kaempferol 3,4'-di-o-β-d-glucoside (6). the identification and structural elucidation of the new compounds were carried out based on spectral data analyses ((1)h a ...201525869735
extract of allium tuberosum rottler ex spreng promoted the hair growth through regulating the expression of igf-1.allium tuberosum rottler ex spreng (atres) has been used as a traditional medicine for the treatment of abdominal pain, diarrhea, and asthma. in this study, we investigated the hair growth promoting activities of atres on telogenic c57bl6/n mice. hair growth was significantly increased in the dorsal skin of ethanol extract of atres treated mouse group compared with the control mouse group. to enrich the hair promoting activity, an ethanol-insoluble fraction was further extracted in sequence with ...201526078771
de novo assembly and annotation of the chinese chive (allium tuberosum rottler ex spr.) transcriptome using the illumina platform.chinese chive (a. tuberosum rottler ex spr.) is one of the most widely cultivated allium species in china. however, minimal transcriptomic and genomic data are available to reveal its evolution and genetic diversity. in this study, de novo transcriptome sequencing was performed to produce large transcript sequences using an illumina hiseq 2000 instrument. we produced 51,968,882 high-quality clean reads and assembled them into 150,154 contigs. a total of 60,031 unigenes with an average length of ...201526204518
de novo sequencing and characterization of the bradysia odoriphaga (diptera: sciaridae) larval transcriptome.the most serious pestilent threat to the chinese chive, allium tuberosum rottle ex spreng (liliaceae) is the bradysia odoriphaga yang and zhang. there is limited genetic research focused on b. odoriphaga, partially due to the lack of genomic resources. the advent of high-throughput sequencing technologies has enabled generation of genomic resources in a short time frame and at minimal costs. in this study, we performed, for the first time, de novo transcriptome sequencing of the b. odoriphaga. h ...201526219018
a fomitopsis pinicola jeseng formulation has an antiobesity effect and protects against hepatic steatosis in mice with high-fat diet-induced obesity.this study investigated the antiobesity effect of an extract of the fomitopsis pinicola jeseng-containing formulation (fava), which is a combination of four natural components: fomitopsis pinicola jeseng; acanthopanax senticosus; viscum album coloratum; and allium tuberosum. high-fat diet- (hfd-) fed male c57bl/6j mice were treated with fava (200 mg/kg/day) for 12 weeks to monitor the antiobesity effect and amelioration of nonalcoholic fatty liver diseases (nafld). body and white adipose tissue ...201627200103
a new phenylpropanoid glucoside and a chain compound from the roots of allium tuberosum.a new phenylpropanoid glucoside tuberosinine d (1) and a chain compound (z)-11r,12s,13s-trihydroxy-9-octadecenoate (2) were isolated from the roots of allium tuberosum. the absolute configuration of 1 was established by comparing of experimental and calculated electronic circular dichroism. the absolute configuration of 2 was determined using the modified mosher's method for the first time.201727454896
the effects of applying sewage sludge into jiangxi red soil on the growth of vegetables and the migration and enrichment of cu and zn.jiangxi red soil was used as the tested soil and water spinach (ipomoea aquatic) and chinese chive (allium tuberosum) were used as the tested vegetables in this study to investigate the effects of different amounts of sewage-sludge application on the growth of vegetables and the migration and enrichment patterns of cu and zn in vegetables using the potted method. the results indicated that the application of sewage sludge could improve the properties of red soil and promote vegetable growth. the ...201627579018
in vitro propagation of allium tuberosum rottl. ex. spreng. by shoot proliferation.halved shoot bases of allium tuberosum rottl. ex spreng. proliferated both axillary and adventitious shoots on b5 medium (1968) supplemented with either 6-benzylaminopurine (0.5 mg/l) or 1-naphthalene acetic acid (0.1 mg/l) and 2-isopentenyladenine (0.5 mg11). ia vitro shoots proliferated further numerous shoots upon subculture to fresh medium, and these shoots rooted spontaneously. plantlets were transplanted successfully to soil and retained the diploid condition of the parents.199224201443
in vitro propagation of allium tuberosum rottl. ex. spreng. by shoot proliferation.seeds of trifoliate orange (poncirus trifoliata (l.) raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. axes could be successfully cryopreserved in liquid nitrogen (-196°c) for eight months. the viable embryonic axes exhibited good growth on modified murashige and skoog medium supplemented with 1-naphthalene acetic acid (na ...199224202989
cryopreservation of embryonic axes of trifoliate orange (poncirus trifoliata [l.] raf.).halved shoot bases of allium tuberosum rottl. ex spreng. proliferated both axillary and adventitious shoots on b5 medium (1968) supplemented with either 6-benzylaminopurine (0.5 mg/l) or 1-naphthalene acetic acid (0.1 mg/l) and 2-isopentenyladenine (0.5 mg/l). in vitro shoots proliferated further numerous shoots upon subculture to fresh medium, and these shoots rooted spontaneously. plantlets were transplanted successfully to soil and retained the diploid condition of the parents.199224202987
highly sensitive optical interferometric technique reveals stress-dependent instantaneous nanometric growth fluctuations of chinese chive leaf under heavy metal stress.plant growth apart from being a complex and highly dynamic is dependent on its immediate environment. leaf expansion measurements using statistical interferometry technique, a sensitive interferometric technique at nanometric accuracy and at sub-second levels revealed the presence of characteristic nanometric intrinsic fluctuations [plant biotechnology 31, 195 (2014)]. in this paper, we demonstrate that the nanometric intrinsic fluctuations are sensitive enough that they change under exposure of ...201727915147
isolation, colonization, and chlorpyrifos degradation mediation of the endophytic bacterium sphingomonas strain hjy in chinese chives (allium tuberosum).the endophyte-plant interaction can benefit the host in many different ways. an endophytic bacterium strain (hjy) capable of degrading chlorpyrifos (cp) was isolated from chinese chives (allium tuberosum rottl. ex spreng). the isolated bacterium hjy classified as sphingomonas sp. strain hjy could use cp as the sole carbon source. after being marked with the gfp gene, the colonization and distribution of strain hjy-gfp were directly observed in different tissues of chinese chives with a confocal ...201728103027
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