| amino acid sequence of the n-terminal domain of yam (dioscorea japonica) aerial tuber acidic chitinase. evidence for the presence of a wheat germ agglutinin domain in matured acidic chitinase from unstressed tuber. | the amino acid sequence of the n-terminal domain of acidic chitinase from unstressed aerial tuber was determined and proved the presence of an n-terminal domain in acidic chitinase. the amino acid sequence was determined on a pyroglutamylaminopeptidase-treated n-terminal fragment of v8 protease and on chymotryptic peptides of this fragment. the sequence determined revealed 8 residues deletion and 2 residues insertion as compared with the n-terminal domain of tobacco basic chitinase. the n-termin ... | 1992 | 1623187 |
| [a study of itch intensity in relation to two-point tactual discrimination]. | this study was designed to investigate whether or not the areas of the human body most sensitive to itch stimuli are identical with the body parts least sensitive to tactual discrimination, a hypothesis which arouse from our previous studies of itching. subjects were 12 college students (six males and six females). the itching was induced by grated japanese yam. the body areas were the upper arm (with large two-point thresholds), the lower leg (with intermediate two-point thresholds), and the mi ... | 1986 | 3807060 |
| nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in papua new guinea. | we determined the 3'-termimus 1353 nucleotides (nts) in length excluding the poly (a) tail of yam mild mosaic potyvirus (ymmv) rna. the sequence starts within a long open reading frame (orf) 1209 nts and is followed by untranslated region (3'-utr) of 144 nts. the coat protein (cp) contains 266 amino acids (aa) with molecular ratio (mr) of approximately 30 kda. the cp of ymmv differs substantially from yam mosaic virus (ymv), japanese yam mosaic virus (jymv) (57 and 61% of amino acid sequence ide ... | 1999 | 10481747 |
| characterisation of a potyvirus and a potexvirus from chinese scallion. | molecular analyses of viruses infecting chinese scallion (allium chinense g. don) showed that the plants did not contain any of the poty-, carla- or allexiviruses that are common in garlic plants in china. the complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent different viruses from any in the databases. they could be transmitted mechanically to scallion but not to other allium species (including garlic) or to narcissus. the potyvirus, tentativel ... | 2002 | 12038680 |
| potyviruses, novel and known, in cultivated and wild species of the family apiaceae in australia. | three potyviruses were identified by gene sequencing and found to be widespread in species of apiaceae in australia. only celery mosaic virus was found in celery crops and in one of 180 specimens of feral carrot ( daucus carota). another related but distinct novel potyvirus, carrot virus y, was the only virus found in carrot crops and all except one feral carrot. a more distantly related novel potyvirus, apium virus y, was found in plants of sea celery ( apium prostratum), cultivated parsley ( p ... | 2002 | 12376749 |
| the phylogeny of turnip mosaic virus; comparisons of 38 genomic sequences reveal a eurasian origin and a recent 'emergence' in east asia. | the genomes of a representative world-wide collection of 32 turnip mosaic virus (tumv) isolates were sequenced and these, together with six previously reported sequences, were analysed. at least one-fifth of the sequences were recombinant. in phylogenetic analyses, using genomic sequences of japanese yam mosaic virus as an outgroup, the tumv sequences that did not show clear recombination formed a monophyletic group with four well-supported lineages. these groupings correlated with differences i ... | 2003 | 12859632 |
| characterization of the yam tuber storage proteins from dioscorea batatas exhibiting unique lectin activities. | four major proteins designated db1, db2, db3, and db4 were isolated and characterized from the yam tuber dioscorea batatas. the ratios of their yields were 20:50:20:10. db1 was a mannose-binding lectin (20 kda) consisting of 10-kda subunits and was classified as the monocot mannose-binding lectin family. db2, accounting for 50% of the total protein, was the storage protein, commonly called dioscorins consisting of a 31-kda subunit. on the basis of amino acid sequence, db2 was classified to be di ... | 2004 | 15047697 |
| herbal formulation, yukmi-jihang-tang-jahage, regulates bone resorption by inhibition of phosphorylation mediated by tyrosine kinase src and cyclooxygenase expression. | anti-bone resorption properties of the korean herbal medicine, yukmi-jihang-tang (yj), which is comprised of seven herbs such as rehmannia glutinosa libosch, dioscorea japonica thunb, cornus officinalis sieb et. zucc, smilax glabra roxb, paeonia suffruticosa andr, alisma platago-aquatica var. orientale samuels and hominis placenta, were investigated. cyclooxygenase-2 (cox-2) and tyrosine kinase involve on prostaglandin e2 (pge2) production in mouse calvarial osteoblasts stimulated by cytokine in ... | 2006 | 16513308 |
| konjak mosaic virus: the complete nucleotide sequence of the genomic rna and its comparison with other potyviruses. | konjak mosaic virus (komv) belongs to the genus potyvirus, family potyviridae. the complete nucleotide sequence of komv f isolate (komv f) was determined. the genome is 9,544 nucleotides long excluding the 3' terminal poly a tail and encodes a typical potyviral 350-kda polyprotein of 3,087 amino acids. phylogenetic analysis using known potyvirus polyproteins shows that komv constitutes a branch with yam mosaic virus, close to another branch including japanese yam mosaic virus, turnip mosaic viru ... | 2006 | 16538420 |
| yam storage protein dioscorins from dioscorea alata and dioscorea japonica exhibit distinct immunomodulatory activities in mice. | the aim of this study was to elucidate the effect of the major storage protein dioscorin isolated from two different yam species, tainong no. 1 (tn1-dioscorins) and japanese yam (dj-dioscorins), on the immune activities of mice. dj-dioscorins, like tn1-dioscorins, could induce expression of the pro-inflammatory cytokines and stimulate phagocytosis of raw 264.7. intraperitoneal injection of the tn1-dioscorins into mice stimulated phagocytosis of bone marrow, spleen, and thymic cells. in contrast, ... | 2009 | 19378946 |
| yam (dioscorea pseudojaponica yamamoto) ameliorates cognition deficit and attenuates oxidative damage in senescent mice induced by d-galactose. | this study attempted to access the neuroprotective effect of yam (dioscorea pseudojaponica yamamoto) on the senescent mice induced by d-gal. the mice in the experiments were administered orally with yam (20, 100 or 500 mg/kg for 4 weeks, from the sixth week). the learning and memory abilities of the mice in morris water maze test and the mechanisms involved in the neuroprotective effect of yam on the mice brain tissue were investigated. the content of diosgenin in the yam was also detected by us ... | 2009 | 19885949 |
| furostanol saponins from the rhizomes of dioscorea japonica and their effects on ngf induction. | the rhizome of dioscorea japonica is a food and medicinal source known as 'san yak' in korea. two new furostanol saponins, coreajaponins a (1) and b (2), together with 10 known compounds (3-12) were isolated from the rhizomes of d. japonica. their structures were determined by spectroscopic methods, including 1d and 2d nmr techniques, hrms, and chemical methods. nerve growth factor (ngf), a crucial factor for neuronal survival and differentiation, can potentially improve neurodegenerative diseas ... | 2011 | 21353549 |
| neurotrophic activity of da-9801, a mixture extract of dioscorea japonica thunb. and dioscorea nipponica makino, in vitro. | dioscorea japonica thunb. has been traditionally used to treat polyuria and diabetes in korea. | 2011 | 21651968 |
| withanolides from the rhizomes of dioscorea japonica and their cytotoxicity. | edible yams are tropical crops that serve as important staple foods in many parts of the world. the rhizome of dioscorea japonica , well-known as "japanese yam", is a food and medicinal source known as "san yak" in korea. bioassay-guided fractionation and chemical investigation of the extract of this yam resulted in the identification of two new withanolides, named dioscorolide a (1) and dioscorolide b (2). the structures of these new compounds were determined by spectroscopic methods, including ... | 2011 | 21661726 |
| Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica. | Dioscorin, the major tuber storage protein of yam, has been shown to possess carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and monodehydroascorbate reductase activities. In the present study, dioscorin from Dioscorea japonica was confirmed as a glycoprotein using the enhanced concanavalin A-peroxidase staining method, and the protein was shown to have both N- and O-glycans. Following the gene cloning, four full-length isoforms of dioscorin were expressed in Escherichia coli ... | 2012 | 22195572 |
| crystallization and preliminary x-ray crystallographic analysis of dioscorin from dioscorea japonica. | dioscorin, the major tuber storage protein in yam, has been reported to possess carbonic anhydrase, trypsin inhibitor, angiotensin-converting enzyme (ace) inhibitor, free-radical scavenger, dehydroascorbate reductase and monodehydroascorbate reductase activities. recent research has also found that dioscorin can enhance immune modulation via the toll-like receptor 4 (tlr-4) signal transduction pathway in raw 264.7 cells, murine bone-marrow cells and human monocytes ex vivo. resolving the structu ... | 2012 | 22297997 |
| recombinant dioscorins of the yam storage protein expressed in escherichia coli exhibit antioxidant and immunomodulatory activities. | dioscorins, the major storage proteins in yam tubers, exhibit biochemical and immunomodulatroy activities. to investigate the potential application of dioscorins in biomedical research, we expressed the dioscorin genes dj-dioa3 and dp-dioa2 from dioscorea japonica and dioscorea pseudojaponica, respectively, in e. coli and routinely obtained approximately 15 mg proteins per liter escherichia coli culture (mg/l) to 30 mg/l of rdj-dioscorina3 and 4 to 8 mg/l of rdp-dioscorina2. western blot analyse ... | 2012 | 22796748 |
| molecular cloning, structural analysis and mass spectrometric identification of native dioscorins of various yam species. | dioscorins are the major storage proteins of yam tubers. however, the molecular nature of their heterogeneity in tubers has not been fully elucidated. in this study the authors isolated the dioscorin gene families of dioscorea japonica and dioscorea pseudojaponica, performed matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof-ms) and elucidated which dioscorin isoforms are the major constituents in tubers. | 2013 | 22806688 |
| compositional and conformational analysis of yam proteins by near infrared fourier transform raman spectroscopy. | fourier transform (ft)-raman spectroscopy was employed to study the molecular structure of yam proteins isolated from three commonly consumed yam species including dioscorea alata l., d. alata l. var. purpurea, and dioscorea japonica. although d. alata l. and d. alata l. var. purpurea consisted of similar amino acid residues, they still exhibited significant differences in conformational arrangement. the secondary structure of d. alata l. was mainly an alpha-helix, while d. alata l. var. purpure ... | 2004 | 15612816 |
| functional properties of dioscorin, a soluble viscous protein from japanese yam (dioscorea opposita thunb.) tuber mucilage tororo. | a soluble viscous protein was purified from yam (dioscorea opposita thunb.) tuber mucilage tororo by chromatographic steps, and its functional properties were estimated. the purified dioscorin having the molecular weight of about 200 kda exhibited high scavenging activities against hydroxyl radicals (ic50 = 195.1 microg/ml) and superoxide anion radicals (ic50 = 92.7 microg/ml). moreover, it showed extremely high angiotensin i-converting enzyme inhibitory activity (ic50 = 41.1 microg/ml). the res ... | 2016 | 17294688 |
| chitinophaga polysaccharea sp. nov., an exopolysaccharide-producing bacterium isolated from the rhizoplane of dioscorea japonica. | a novel strain designated mrp-15(t), belonging to the class sphingobacteriia (phylum bacteroidetes), was isolated from the rhizoplane of dioscorea japonica in south korea and was characterized taxonomically using a polyphasic approach. the strain was found to comprise gram-stain-negative, aerobic, non-motile, non-spore-forming rods. phylogenetic analysis based on 16s rrna gene sequences indicated that the strain belonged to the genus chitinophaga but was clearly separated from established specie ... | 2014 | 24006482 |
| complete genome sequence of a divergent strain of japanese yam mosaic virus from china. | a novel strain of japanese yam mosaic virus (jymv-cn) was identified in a yam plant with foliar mottle symptoms in china. the complete genomic sequence of jymv-cn was determined. its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. its organization is virtually identical to that of two jymv isolates from japan. with the latter, it shares nucleotide sequence identities of only 74.7-74.8 %, indicating it might be a member of a new species. however, sequence analysis ... | 2015 | 25244960 |
| detection of japanese yam mosaic virus by rt-lamp. | arapid and simple procedure is described to detect the genomic rna molecule of japanese yam mosaic potyvirus (jymv). this method, named rt-lamp, allows direct detection of rna from infected plants without careful rna extraction, rapid thermal cycling and gel electrophoresis. rt-lamp was successfully applied to leaves, propagules and roots of japanese yam infected with jymv. one of the characteristics of the rt-lamp method is its ability to synthesize an extremely large amount of dna. accordingly ... | 2003 | 14505084 |
| complete nucleotide sequence of the genomic rna of a japanese yam mosaic virus, a new potyvirus in japan. | we determined the complete nucleotide sequence of a potyvirus purified from a japanese yam plant. the genomic rna of this virus is 9,757 nucleotides (nts) in length, excluding the 3'-terminal poly(a) tail. it contains a single open reading frame (orf) encoding a polyprotein of 3130 amino acids (aa) with a calculated mr of 356,793. the genomic organization of this potyvirus is similar to that of other members of the genus potyvirus and nine potential cleavage sites for the viral proteinase were f ... | 1999 | 10470250 |
| taiwanese and japanese yam (dioscorea spp.) extracts attenuate doxorubicin-induced cardiotoxicity in mice. | the present study was designed to explore whether yam could protect the heart from doxorubicin (dox)-induced oxidative stress leading to cardiotoxicity in vivo. in this study, the protective effects of water and ethanol extracts of three varieties of yam, including water extracts of dioscorea japonica thunb., ethanol extracts of d. japonica thunb., water extracts of dioscorea alata, ethanol extracts of d. alata, water extracts of dioscorea purpurea, and ethanol extracts of d. purpurea, against d ... | 2017 | 28987364 |
| consumption of japanese yam improves lipid metabolism in high-cholesterol diet-fed rats. | we investigated the effects of dietary japanese yam (dioscorea japonica thunb.) on lipid metabolism. male wistar rats (6 wk old) were fed a high-cholesterol diet for 6 wk and then supplemented with 26% of japanese yam or 0.5% of its constituent diosgenin for a further 4 wk of high-cholesterol feeding (c6-j4 and c6-d4 groups, respectively). in the c6-j4 group, body weight gains significantly decreased, but skeletal muscle fiber sizes in quadriceps significantly increased compared with the other g ... | 2016 | 27928123 |
| combined application of uhplc-qtof/ms, hplc-elsd and 1 h-nmr spectroscopy for quality assessment of da-9801, a standardised dioscorea extract. | da-9801, a standardised 50% aqueous ethanolic extract of a mixture of dioscorea japonica and d. nipponica, is a botanical drug candidate for the treatment of diabetic neuropathy, which finished its us phase ii clinical trials recently. an advanced quality control method is needed for further development of da-9801, considering its high contents of both primary and secondary metabolites. | 2017 | 27910174 |
| amelioration of auditory response by da9801 in diabetic mouse. | diabetes mellitus (dm) is a metabolic disease that involves disorders such as diabetic retinopathy, diabetic neuropathy, and diabetic hearing loss. recently, neurotrophin has become a treatment target that has shown to be an attractive alternative in recovering auditory function altered by dm. the aim of this study was to evaluate the effect of da9801, a mixture of dioscorea nipponica and dioscorea japonica extracts, in the auditory function damage produced in a stz-induced diabetic model and to ... | 2015 | 25878713 |
| da-9801 promotes neurite outgrowth via erk1/2-creb pathway in pc12 cells. | in the present study, we examined the mechanisms underlying the effect of da-9801 on neurite outgrowth. we found that da-9801 elicits its effects via the mitogen-activated protein kinase (mek) extracellular signal-regulated kinase (erk)1/2-camp response element-binding protein (creb) pathway. da-9801, an extract from a mixture of dioscorea japonica and dioscorea nipponica, was reported to promote neurite outgrowth in pc12 cells. the effects of da-9801 on cell viability and expression of neuronal ... | 2015 | 25482167 |
| alteration in brain-derived neurotrophic factor (bdnf) after treatment of mice with herbal mixture containing euphoria longana, houttuynia cordata and dioscorea japonica. | literature data indicate that brain-derived neurotrophic factor (bdnf), cyclic-amp response element-binding protein (creb) and phospho-creb (pcreb) may have a place in depression. bdnf belongs to the neurotrophin family that plays an important role in proliferation, survival and differentiation of different cell populations in the mammalian nervous system. the herbal mixture used in the present study consists of euphoria longana, houttuynia cordata and dioscorea japonica. the purpose of the pres ... | 2014 | 25431319 |
| dioscorea extract (da-9801) modulates markers of peripheral neuropathy in type 2 diabetic db/db mice. | the purpose of this study was to investigate the therapeutic effects of da-9801, an optimized extract of dioscorea species, on diabetic peripheral neuropathy in a type 2 diabetic animal model. in this study, db/db mice were treated with da-9801 (30 and 100 mg/kg, daily, p.o.) for 12 weeks. da-9801 reduced the blood glucose levels and increased the withdrawal latencies in hot plate tests. moreover, it prevented nerve damage based on increased nerve conduction velocity and ultrastructural changes. ... | 2014 | 25414776 |
| dioscorea japonica extract down-regulates prostaglandin e2 synthetic pathway and induces apoptosis in lung cancer cells. | prostaglandin e2 plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. prostaglandin e2 is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin e synthase. in some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin e synthase-1 are transiently induced, leading to prostaglandin e2 overproduction. the present study showed that dioscorea japonica extract suppresses mrna expression of ... | 2014 | 25411520 |
| the protective effects of da-9801 (dioscorea extract) on the peripheral nerves in streptozotocin-induced diabetic rats. | it has been reported that da-9801, an extract mixture of dioscorea japonica thunb and dioscorea nipponica makino, produces a neurotrophic activity. therefore, this study was conducted to examine the neuroprotective effects of da-9801 in streptozotocin-induced diabetic rats. the experimental rats were divided into six groups: the control group, group i (non-diabetic rats treated with da-9801), group ii (diabetic, non-treated rats) and groups iii, iv, and v (diabetic rats treated with da-9801 at d ... | 2013 | 24418878 |
| antioxidant and anti-inflammatory properties of taiwanese yam (dioscorea japonica thunb. var. pseudojaponica (hayata) yamam.) and its reference compounds. | dioscorea japonica thunb. var. pseudojaponica (dp) is consumed as food and widely used in traditional chinese medicine in taiwan. the aims of this study are to investigate the antioxidant and anti-inflammatory effects of ethanol extract of dp (edp) and its reference compounds. fingerprint chromatogram from hplc indicated that edp contains gallic acid and vanillic acid. edp was evaluated for its antioxidant effects and lps-induced nitrite oxide (no) production in raw264.7 cells. edp decreased the ... | 2013 | 23790890 |
| therapeutic potential of dioscorea extract (da-9801) in comparison with alpha lipoic acid on the peripheral nerves in experimental diabetes. | da-9801, a mixture of extracts from dioscorea japonica thunb. and dioscorea nipponica makino, was reported to have neurotrophic activity. therefore, we investigated the therapeutic potential of da-9801, in comparison with alpha lipoic acid (ala), for peripheral nerves preservation in experimental diabetes. experimental animals were divided into 4 groups, and each group was designated according to the type of treatment administered as follows: normal, dm, dm+da-9801, and dm+ala. after 16 weeks, r ... | 2013 | 23671883 |
| influence of different particle processing on hypocholesterolemic and antiatherogenic activities of yam (dioscorea pseudojaponica) in cholesterol-fed rabbit model. | nanoparticle processing is implicated in enhancing bioactive or nutritional compound release from raw foods. the aim of the present study was to evaluate whether different particle processing might affect the lipid-lowering activity of dioscorea pseudojaponica (dp) and to investigate whether dp could be a potential functional food for prevention of atherogenesis. its possible molecular mechanisms were also evaluated. | 2013 | 23400896 |
| natto and viscous vegetables in a japanese-style breakfast improved insulin sensitivity, lipid metabolism and oxidative stress in overweight subjects with impaired glucose tolerance. | we previously suggested that the consumption of natto and viscous vegetables as part of a japanese-style meal based on white rice (wr) reduced postprandial glucose and insulin levels in healthy subjects. the aim of the present study was to assess whether a single breakfast of natto and viscous vegetables or the same breakfast consumed for 2 weeks could improve glucose control, insulin sensitivity, lipid metabolism and oxidative stress in overweight subjects with impaired glucose tolerance (igt). ... | 2012 | 21899797 |
| putative storage root specific promoters from cassava and yam: cloning and evaluation in transgenic carrots as a model system. | a prerequisite for biotechnological improvements of storage roots is the availability of tissue-specific promoters enabling high expression of transgenes. in this work, we cloned two genomic fragments, pme1 and pdj3s, controlling the expression of a gene with unknown function from cassava (manihot esculenta) and of the storage protein dioscorin 3 small subunit gene from yam (dioscorea japonica), respectively. using beta-glucuronidase as a reporter, the activities of pme1 and pdj3s were evaluated ... | 2010 | 20369359 |
| determination of steroidal saponins in different organs of yam (dioscorea pseudojaponica yamamoto). | yams (dioscorea spp.) are perennial trailing rhizome plants. steroidal saponins, furostanol and spirostanol glycosides are the marked functional compounds in yams. in this investigation, a c18 solid phase extraction method was developed for yam saponins purification. the contents of saponins in various organs of yam (dioscorea pseudojaponica yamamoto) were also determined. results showed that the recoveries of yam saponins extracted by the developed method were about 99.48-100.08% when the sapon ... | 2008 | 26065772 |
| effects of domestic processing on steroidal saponins in taiwanese yam cultivar (dioscorea pseudojaponica yamamoto). | the effects of domestic processing on steroidal saponins and furostanol and spirostanol glycosides in taiwanese yam cultivar (dioscorea pseudojaponica yamamoto) were studied. the baking or frying of yam slices was conducted at 150, 180, and 200 degrees c for 3, 5, and 10 min. yam slices were steamed or microwave cooked at 2450 mhz with an output power of 850 w for 3, 5, and 10 min. the various saponins were quantified by hplc with an evaporative light scattering detector (elsd). results showed t ... | 2006 | 17177526 |
| isolation and identification of steroidal saponins in taiwanese yam cultivar (dioscorea pseudojaponica yamamoto). | a new furostanol pentaoligoside and spirostanol tetraoligoside were isolated for the first time from yam tubers (dioscorea pseudojaponica yamamoto) from taiwan, together with four known yam saponins, methyl protodioscin, methyl protogracillin, dioscin, and gracillin. their structures were characterized as 26-o-beta-d-glucopyranosyl-22alpha-methoxyl-(25r)-furost-5-en-3beta,26-diol, 3-o-alpha-l-rhamnopyranosyl-(1-->2)-o-([alpha-l-rhamnopyranosyl-(1-->4)]-o-[alpha-l-rhamnopyranosyl-(1-->4)])-beta-d ... | 2003 | 14558759 |
| effects of taiwanese yam (dioscorea japonica thunb var. pseudojaponica yamamoto) on upper gut function and lipid metabolism in balb/c mice. | this study investigated the effects of a taiwanese yam, dioscorea japonica thunb var. pseudojaponica yamamoto, on upper gut function and lipid metabolism in adult balb/c mice. | 2013 | 12831952 |
| complete nucleotide sequence of the genomic rna of a mild strain of japanese yam mosaic potyvirus. | we determined the complete nucleotide sequence of a mild strain of japanese yam mosaic potyvirus (jymv-m) and compared it with the published sequence of severe strain of jymv (jymv-j1). the genomic rna of jymv-m is 9,760 nucleotides (nts) in length, excluding the poly (a) tail, and encodes a polyprotein of 3,132 amino acids. among nine potential cleavage sites, only the p1 and nia recognition sites (between 6k1 and ci) had different sequences from those of jymv-j1. the data confirm the strain st ... | 2000 | 10795528 |
| positions of disulfide bonds in yam (dioscorea japonica) acidic class il (class iv) chitinase. | yam acidic class i chitinase belongs to a low molecular weight subclass of class i (class il; corresponds to class iv) chitinase. the positions of disulfide bonds in this chitinase were examined. chitinase protein was digested with acid protease and thermolysin, and the resulting disulfide bond containing peptides were separated by reversed-phase hplc and detected using the sbd-f (7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonic acid ammonium salt) method. four intradisulfide bonds containing peptides ... | 1996 | 8914841 |
| the complete amino acid sequence of yam (dioscorea japonica) chitinase. a newly identified acidic class i chitinase. | the complete amino acid sequence of acidic chitinase from yam (dioscorea japonica) aerial tubers was determined. the protein is composed of a single polypeptide chain of 250 amino acid residues and has a calculated molecular mass of 27,890 da. there is an nh2-terminal domain, a hinge region, and a main structure, typical for class i chitinases (shinshi, h., neuhaus, j.-m., ryals, j., and meins, f., jr. (1990) plant mol. biol. 14, 357-368). we have obtained the first evidence for an acidic class ... | 1992 | 1400311 |
| [itch and the heat tolerance scale: effects of thermographic biofeedback]. | effects of heat tolerance (ht) relating with individual emotionality, and of thermal biofeedback on itching were examined. fourteen high ht subjects and 12 low ht subjects were selected by the cold and heat tolerance scales (dienstbier, laguardia, & wilcox, 1987). the experiment was divided into three phases: rating of itch, eight thermal biofeedback (increase or decrease) training sessions, and rating of itch with biofeedback. itching was produced by a glue made from japanese yam powder. yam gl ... | 1992 | 1507664 |
| isolation and hypoglycemic activity of dioscorans a, b, c, d, e, and f; glycans of dioscorea japonica rhizophors. | | 1986 | 3749337 |