identification of genetic markers linked to banana streak disease expression in inter-specific musa hybrids.recently-introduced inter-specific musa hybrids, bred for improved yield and resistance to diseases, have been found to be widely infected with banana streak virus (bsv), the causal agent of banana streak disease (bsd). one hypothesis suggests: (1) that bsd occurrence in these inter-specific hybrids results from activation of bsv-ol endogenous pararetrovirus sequences (eprv) integrated into the musa genome rather than from external sources of infection, and (2) that the process of genetic hybrid ...200312595986
fungus-elicited metabolites from plants as an enriched source for new leishmanicidal agents: antifungal phenyl-phenalenone phytoalexins from the banana plant (musa acuminata) target mitochondria of leishmania donovani promastigotes.two antifungal phenyl-phenalenone phytoalexins isolated from the banana plant (musa acuminata) elicited with the fungus fusarium oxysporum, together with a methoxy derivative of one of them and two epoxide precursors of their chemical synthesis, were tested for leishmanicidal activity on leishmania donovani promastigotes and l. infantum amastigotes. drugs inhibited proliferation of both forms of the parasite with a 50% lethal concentration range between 10.3 and 68.7 micro g/ml. their lethal mec ...200415105102
creation of a bac resource to study the structure and evolution of the banana (musa balbisiana) genome.the first bacterial artificial chromosome (bac) library of the banana species musa balbisiana 'pisang klutuk wulung' (pkw bac library) was constructed and characterized. one improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. the use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid dna. furthermore, the usefulness of the indu ...200415644977
phenalenone-type compounds from musa acuminata var. "yangambi km 5" (aaa) and their activity against mycosphaerella fijiensis.two perinaphthenone-type compounds (1 and 2) were isolated together with four known phenylphenalenones (3-6) from the rhizomes of musa acuminata var. "yangambi km 5". the structures of the new phenalenones were assigned as 2-hydroxy-1h-phenalen-1-one (1) and 2-methoxy-1h-phenalen-1-one (2) on the basis of their spectroscopic data and were confirmed by synthesis. compounds 1 and 2 displayed significantly enhanced activity against mycosphaerella fijiensis in comparison with other phenylphenalenone ...200717428093
molecular characterization of banana streak acuminata vietnam virus isolated from musa acuminata siamea (banana cultivar).an isolate of banana streak virus (bsv) that does not also occur as an integrant in the musa balbisiana genome was sought in order to investigate the biological role of bsv in the evolution of either the musa genome or of the virus itself. we isolated bsv virions from a musa acuminata siamea accession from vietnam and sequenced the entire viral genome. the molecular organization is similar to that described for other bsv but slightly larger (7801 bp vs. 1611-7568 bp), and orf i has a non-convent ...200717431738
musa acuminata (del monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity.a homodimeric, fructose-binding lectin was isolated from del monte bananas by using a protocol that involved ion-exchange chromatography on deae-cellulose and sp-sepharose, and gel filtration by fast protein liquid chromatography on superdex 75. not only fructose, but also glucose, mannose, rhamnose and glucosamine could inhibit the lectin. the n-terminal amino acid sequence of its identical 15-kda subunits was similar to lectins from other musa species except for the deletion of the n-terminal ...200919195858
phenolics and antioxidant properties of fruit pulp and cell wall fractions of postharvest banana (musa acuminata juss.) cultivars.banana fruits are important foods, but there have been very few studies evaluating the phenolics associated with their cell walls. in the present study, (+) catechin, gallocatechin, and (-) epicatechin, as well as condensed tannins, were detected in the soluble extract of the fruit pulp; neither soluble anthocyanidins nor anthocyanins were present. in the soluble cell wall fraction, two hydroxycinnamic acid derivatives were predominant, whereas in the insoluble cell wall fraction, the anthocyani ...201020553046
analysis of non-tir nbs-lrr resistance gene analogs in musa acuminata colla: isolation, rflp marker development, and physical mapping.many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (r-genes). the largest known family of plant r-genes encode proteins with nucleotide-binding site (nbs) and c-terminal leucine-rich repeat (lrr) domains. conserved motifs in such genes in diverse plant species offer a means for iso ...200818234103
molecular characterization of banana streak virus isolate from musa acuminata in china.banana streak virus (bsv), a member of genus badnavirus, is a causal agent of banana streak disease throughout the world. the genetic diversity of bsvs from different regions of banana plantations has previously been investigated, but there are relatively few reports of the genetic characteristic of episomal (non-integrated) bsv genomes isolated from china. here, the complete genome, a total of 7722bp (genbank accession number dq092436), of an isolate of banana streak virus (bsv) on cultivar cav ...201122160939
identification and expression analysis of four 14-3-3 genes during fruit ripening in banana (musa acuminata l. aaa group, cv. brazilian).to investigate the regulation of 14-3-3 proteins in banana (musa acuminata l. aaa group, cv. brazilian) fruit postharvest ripening, four cdnas encoding 14-3-3 proteins were isolated from banana and designated as ma-14-3-3a, ma-14-3-3c, ma-14-3-3e, and ma-14-3-3i, respectively. amino acid sequence alignment showed that the four 14-3-3 proteins shared a highly conserved core structure and variable c-terminal as well as n-terminal regions with 14-3-3 proteins from other plant species. phylogenetic ...201122009053
analysis of the distribution and structure of integrated banana streak virus dna in a range of musa cultivars.summary banana streak virus strain ol (bsv-ol) commonly infects new musa hybrids, and this infection is thought to arise de novo from integrated virus sequences present in the nuclear genome of the plant. integrated dna (musa6+8 sequence) containing the whole genome of the virus has previously been cloned from cv. obino l'ewai (musa aab group), a parent of many of the hybrids. using a southern blot hybridization assay, we have examined the distribution and structure of integrated bsv-ol sequence ...200120573008
a 3-vinyl cephem derivative, a useful intermediate in the synthesis of cepham antibiotics, from aspergillus awamori associated with banana fruit.aspergillus awamori was isolated from a diseased banana fruit, musa acuminata cv. ambul. the fungus was fermented in potato dextrose broth and on potato dextrose agar media and the fungal media were extracted with etoac. chromatographic separation of the etoac extracts furnished 4-methoxybenzyl 7-phenylacetamido-3-vinyl-3-cephem-4-carboxylate (1), along with three naphtho-γ-pyrones, flavasperone (2), foncesinone a (3) and aurasperone a (4), and three alkaloids, aspernigrin a (5), pestalamide c ( ...201526669099
activity against drug resistant-tuberculosis strains of plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases.tuberculosis (tb) kills about 3 million people per year worldwide. furthermore, tb is an infectious disease associated with hiv patients, and there is a rise in multidrug-resistant tb (mdr-tb) cases around the world. there is a need for new anti-tb agents. the study evaluated the antimycobacterial activity of nine plants used in mexican traditional medicine to treat tuberculosis and other respiratory diseases. nasturtium officinale showed the best activity (mic = 100 microg/ml) against the sensi ...200817726732
highly efficient agrobacterium-mediated transformation of embryogenic cell suspensions of musa acuminata cv. mas (aa) via a liquid co-cultivation system.a high efficient protocol of agrobacterium-mediated transformation of musa acuminata cv. mas (aa), a major banana variety of the south east asia region, was developed in this study. male-flower-derived embryogenic cell suspensions (ecs) were co-cultivated in liquid medium with agrobacterium strain eha105 harboring a binary vector pcambia2301 carrying nptii and gusa gene in the t-dna. depending upon conditions and duration of co-cultivation in liquid medium, 0-490 transgenic plants per 0.5 ml pac ...200717551731
antibacterial and antioxidant activities of musa sp. leaf extracts against multidrug resistant clinical pathogens causing nosocomial investigate different musa sp. leave extracts of hexane, ethyl acetate and methanol were evaluated for antibacterial activity against multi-drug resistant pathogens causing nosocomial infection by agar well diffusion method and also antioxidant activities.201323998016
carbohydrate binding properties of banana (musa acuminata) lectin i. novel recognition of internal alpha1,3-linked glucosyl residues.examination of lectins of banana (musa acuminata) and the closely related plantain (musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the alpha-anomeric form of these sugars. both generate precipitin curves with branched chain alpha-mannans (yeast mannans) and alpha-glucans (glycogens, dextrans, and starches), but not with linear alpha-glu ...200111322880
isolation of (s)-(+)-naproxene from musa acuminata. inhibitory effect of naproxene and its 7-methoxy isomer on constitutive cox-1 and inducible cox-2.the isolation and characterisation of (s)-(+)-6-methoxy-alpha-methyl-2-naphthaleneacetic acid, a well known synthetic non-steroidal anti-inflammatory drug (naproxene), from a natural source is described for the first time. we evaluated the ability of naproxene and its 7-methoxy isomer to abrogate constitutive cox-1 and inducible cox-2 activity in human a549 cells. naproxene inhibited cox-1 (ic50 = 3.42 microm) and cox-2 (ic50 = 1.53 microm), whereas the 7-methoxy isomer had no appreciable effect ...200010909271
genetic repair of mutations in plant cell-free extracts directed by specific chimeric oligonucleotides.chimeric oligonucleotides are synthetic molecules comprised of rna and dna bases assembled in a double hairpin conformation. these molecules have been shown to direct gene conversion events in mammalian cells and animals through a process involving at least one protein from the dna mismatch repair pathway. the mechanism of action for gene repair in mammalian cells has been partially elucidated through the use of a cell-free extract system. recent experiments have expanded the utility of chimeric ...200010859173
the selective increase or decrease of organellar dna in generative cells just after pollen mitosis one controls cytoplasmic inheritance.organellar dna in mature pollen grains of eight angiosperm species (actinidia deliciosa lindl., antirrhinum majus l., arabidopsis thaliana (l.) heynh., medicago sativa l., musa acuminata colla, pelargonium zonale (l.) l'hér, petunia hybrida vilm. and rhododendron mucronatum (blume) g. don, in which the modes of organellar inheritance have been determined genetically, was observed by fluorescence microscopy using technovit 7100 resin sections double-stained with 4',6-diamidino-2-phenylindole (dap ...199910467031
the abundant 31-kilodalton banana pulp protein is homologous to class-iii acidic chitinases.we have identified and characterized the abundant protein from the pulp of banana fruit (musa acuminata cv. grand nain), and have isolated a cdna clone encoding this protein. comparison of the amino terminal sequence of the purified 31 kda protein (p31) suggests that it is related to plant chitinases. western analyses utilizing rabbit anti-p31 antiserum demonstrate that this protein is pulp-specific in banana. a full-length cdna clone homologous to class iii acidic chitinase genes has been isola ...19989461677
wedelolactone and coumestan derivatives as new antihepatotoxic and antiphlogistic principles.for the study of structure-activity relationships, the antihepatotoxic wedelolactone (7-methoxy-5,11,12-trihydroxy-coumestan) and 6 coumestan derivatives were synthesized by the application of a modified method of wanzlich. an evaluation of the biological characteristics of the synthetic compounds and acuminatin from musa acuminata showed that most of the wedelolactone derivatives significantly protected primary cultured liver cells from the toxicity of ccl4, galactosamine (galc), and phalloidin ...19882458108
development of expressed sequence tag and expressed sequence tag-simple sequence repeat marker resources for musa acuminata.banana (musa acuminata) is a crop contributing to global food security. many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. the objective of this study was to develop an expressed sequence tag (est) database of transcripts expressed during compatible and incompatible banana-mycosphaerella fijiensis (mf) interactions. black leaf streak disease (blsd), caused by mf, is a destructive disease of banana. microsatellite markers were developed as a resourc ...201223240072
isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit.identification of ethylene-regulated and ripening-related genes from banana (musa acuminata var. harichaal) fruits using ddrt-pcr led to the isolation of differentially expressed partial cdna of pectin methylesterase inhibitor (mapmei) gene. its full-length cdna sequence consisted of a 567 bp orf, encoding a protein of 189 aa with deduced molecular mass 19.6 kda. expression pattern of mapmei gene revealed that upon ethylene treatment, this gene is up-regulated initially giving maximum expression ...201223573057
dietary administration of banana (musa acuminata) peel flour affects the growth, antioxidant status, cytokine responses, and disease susceptibility of rohu, labeo explore the feasibility of musa acuminata (banana) peels as a feed additive, effects of banana peel flour (bpf) on the growth and immune functions of labeo rohita were evaluated. diets containing five different concentrations of bpf (0% [basal diet], 1% [b1], 3% [b3], 5% [b5], and 7% [b7]) were fed to the fish (average weight: 15.3 g) for 60 days. the final weight gain and specific growth rate were higher (p < 0.05) in the b5 group. the most significant improvements in immune parameters such ...201627294156
in vitro colonic fermentation and glycemic response of different kinds of unripe banana flour.this work aimed to study the in vitro colonic fermentation profile of unavailable carbohydrates of two different kinds of unripe banana flour and to evaluate their postprandial glycemic responses. the unripe banana mass (ubm), obtained from the cooked pulp of unripe bananas (musa acuminata, nanicão variety), and the unripe banana starch (ubs), obtained from isolated starch of unripe banana, plantain type (musa paradisiaca) in natura, were studied. the fermentability of the flours was evaluated b ...201020839056
local phytochemical response of musa acuminata × balbisiana colla cv. 'bluggoe' (abb) to colonization by sternorrhyncha.the interaction of two sternorrhyncha species, the banana aphid (pentalonia nigronervosa coquerel (hemiptera: aphididae, aphidinae)), vector of the banana bunchy top virus (bbtv), and the latania scale (hemiberlesia lataniae signoret (hemiptera: diaspididae, diaspidinae)) with musa acuminata × balbisiana colla (abb group) 'bluggoe' (musaceae) was investigated by a combination of conventional and spatially resolved analytical techniques, (1)h nmr, uhplc-ms, and matrix-free uv-laser desorption/ion ...201727839785
citric acid production by koji fermentation using banana peel as a novel substrate.the growing demand for citric acid and the current need for alternative sources have encouraged biotechnologists to search for novel and economical substrates. koji fermentation was conducted using the peels of banana (musa acuminata) as an inexpensive substrate for the production of citric acid using aspergillus niger. various crucial parameters that affect citric acid production such as moisture content, temperature, ph, inoculum level and incubation time were quantified. moisture (70%), 28 de ...201020219361
a lectin isolated from bananas is a potent inhibitor of hiv replication.banlec is a jacalin-related lectin isolated from the fruit of bananas, musa acuminata. this lectin binds to high mannose carbohydrate structures, including those found on viruses containing glycosylated envelope proteins such as human immunodeficiency virus type-1 (hiv-1). therefore, we hypothesized that banlec might inhibit hiv-1 through binding of the glycosylated hiv-1 envelope protein, gp120. we determined that banlec inhibits primary and laboratory-adapted hiv-1 isolates of different tropis ...201020080975
structural analysis of catalase from two musa accessions, fhia18 and williams, and from ravenala madagascariensis.nucleotide sequences of catalase were obtained following amplification using specific primers and were blasted against musa acuminata catalase 2 mrna from ncbi (157418810). clustering of the amino acid sequences from ncbi was done using clustal x. the latter revealed that fhia18 catalase is more related to ravenala madagascariensis (musa relative) catalase while the williams catalase is more related to a clade containing a musa acuminata (musa ancestor) catalase from ncbi. the tertiary structure ...200819374128
cloning and expression of resistance gene analogs (rgas) from wild banana resistant to banana fusarium wilt.wild banana species are essential natural gene pools for banana improvement. in this study, six rgas about 500 bp were obtained from leaves of musa acuminata, a wild banana shown to be resistant to banana fusarium wilt race 4, by pcr amplification with degenerate primers designed according to the conserved nbs motif and serine/threonine kinase domain of plant resistance (r) genes. among these rgas, the deduced amino acids of wnb1 and wnb2 contain nb-arc domain and wnb1 can be translated into pol ...200718349511
analysis of the sugar-binding specificity of mannose-binding-type jacalin-related lectins by frontal affinity chromatography--an approach to functional classification.the jacalin-related lectin (jrl) family comprises galactose-binding-type (gjrls) and mannose-binding-type (mjrls) lectins. although the documented occurrence of gjrls is confined to the family moraceae, mjrls are widespread in the plant kingdom. a detailed comparison of sugar-binding specificity was made by frontal affinity chromatography to corroborate the structure-function relationships of the extended mjrl subfamily. eight mjrls covering a broad taxonomic range were used: artocarpin from art ...200818266762
molecular characterisation of endophytic fungi from roots of wild banana (musa acuminata).endophytic fungi inhabit apparently healthy plant tissues and are prevalent in terrestrial plants, especially root tissues, which harbour a wide assemblage of fungal endophytes. therefore, this study focused on the isolation and characterisation of endophytic fungi from the roots of wild banana (musa acuminata). a total of 31 isolates of endophytic fungi were isolated from 80 root fragments. the endophytic fungi were initially sorted according to morphological characteristics and identified usin ...201627019688
molecular cloning and expression of hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral and wounding/herbivory induced leaf volatile sesquiterpenoids.farnesyl pyrophosphate synthase (fpps ec catalyzes the production of farnesyl pyrophosphate (fpp), which is a key precursor for many sesquiterpenoids such as floral scent and defense volatiles against herbivore attack. here we report a new full-length cdna encoding farnesyl diphosphate synthase from hedychium coronarium. the open reading frame for full-length hcfpps encodes a protein of 356 amino acids, which is 1068 nucleotides long with calculated molecular mass of 40.7 kda. phylogen ...201323333605
construction and characterization of a plant transformation-competent bibac library of the black sigatoka-resistant banana musa acuminata cv. tuu gia (aa).a plant transformation-competent binary bacterial artificial chromosome (bibac) library was constructed from musa acuminata cv. tuu gia (aa), a black sigatoka-resistant diploid banana. after digestion of high-molecular-weight banana dna by hindiii, several methods of dna size selection were tested, followed by ligation, using a vector/insert molar ratio of 4:1. the library consists of 30,700 clones stored in 80 384-well microtiter plates. the mean insert size was estimated to be 100 kb, and the ...200515650812
light- and singlet oxygen-mediated antifungal activity of phenylphenalenone phytoalexins.the light-induced singlet oxygen production and antifungal activity of phenylphenalenone phytoalexins isolated from infected banana plants (musa acuminata) are reported. upon absorption of light energy all studied phenylphenalenones sensitise the production of singlet oxygen in polar and non-polar media. antifungal activity of these compounds towards fusarium oxysporum is enhanced in the presence of light. these results, together with the correlation of ic50 values under illumination with the qu ...200415239009
effect of menadione sodium bisulfite, an inducer of plant defenses, on the dynamic of banana phytoalexin accumulation during pathogenesis.using an authentic sample of 2-hydroxy-9-(p-hydroxyphenyl)-phenalen-1-one, a banana phenalenone-type phytoalexin, we studied its dynamic of accumulation during pathogenesis of banana plants (musa acuminata (aaa), grand nain) inoculated with fusarium oxysporum f.sp. cubense (foc), race 4, the causal agent of panama disease. the results obtained demonstrate that banana plants treated prior inoculation with menadione sodium bisulfite (msb), an inducer of plant defenses, are capable of changing the ...200312926878
fruit-specific lectins from banana and of the predominant proteins in the pulp of ripe bananas (musa acuminata l.) and plantains (musa spp.) has been identified as a lectin. the banana and plantain agglutinins (called banlec and planlec, respectively) were purified in reasonable quantities using a novel isolation procedure, which prevented adsorption of the lectins onto insoluble endogenous polysaccharides. both banlec and planlec are dimeric proteins composed of two identical subunits of 15 kda. they readily agglutinate rabbit e ...200011030554
evolution and structural diversification of nictaba-like lectin genes in food crops with a focus on soybean (glycine max).the nictaba family groups all proteins that show homology to nictaba, the tobacco lectin. so far, nictaba and an arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. the availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of nictaba-like genes in 15 species, representing members of the fabaceae, poaceae, solanaceae, musaceae, arecaceae, malvaceae and rubiaceae. additionally, phylogenetic relations ...201728087663
identification and chromosomal localization of the monkey retrotransposon in musa sp.retroelements are ubiquitous features of eukaryotic genomes, often accounting for a substantial fraction of their total dna content. one major group of retroelements, which includes the gypsy and copia-like elements, is distinguished by the presence of long terminal repeats (ltrs). we have identified and partially characterized a sequence from banana (musa acuminata cv. grand nain) which shows significant homology to gypsy-like ltr retroelements from other species. the element, named monkey, sho ...200010954075
the effect of banana (musa acuminata) peels hot-water extract on the immunity and resistance of giant freshwater prawn, macrobrachium rosenbergii via dietary administration for a long term: activity and gene transcription.the non-specific immune parameters, disease resistance and immune genes expressions in macrobrachium rosenbergii were evaluated at 120 days of post feeding the diets containing the extracts of banana, musa acuminate, fruit's peel (banana peels extract, bpe) at 0, 1.0, 3.0 and 6.0 g kg(-1). results showed that prawns fed with a diet containing bpe at the level of 1.0, 3.0 and 6.0 g kg(-1) for 120 days had a significantly higher survival rate (30.0%, 40.0% and 56.7%, respectively) than those fed w ...201526118934
dietary supplement of banana (musa acuminata) peels hot-water extract to enhance the growth, anti-hypothermal stress, immunity and disease resistance of the giant freshwater prawn, macrobrachium the present study, macrobrachium rosenbergii were fed with diets containing extracts of banana, musa acuminate, fruit's peel (banana peels extract, bpe) at 0, 1.0, 3.0 and 6.0 g kg(-1). the non-specific immune parameters, disease resistance and anti-hypothermal stress were evaluated at 2, 4, 8, 16 and 32 days of post feeding. also, we demonstrated the percent weight gain (pwg), percent length gain (plg), feeding efficiency (fe), and survival rate of giant freshwater prawn at 30, 60, 90, and 1 ...201525634258
effects of hot-water extract of banana (musa acuminata) fruit's peel on the antibacterial activity, and anti-hypothermal stress, immune responses and disease resistance of the giant freshwater prawn, macrobrachium rosenbegii.the hot-extracts isolated from fruit's peel of banana, musa acuminata, was evaluated on the antibacterial activity to pathogens from aquatic animals, and immunostimulating potential, disease resistance and anti-hypothermal stress in giant freshwater prawn, macrobrachium rosenbergii through injection administration. the banana peel extract (bpe) showed good activity against 1 gram-positive and 3 gram-negative pathogens, including lactococcus garvieae, photobacteria damsella, vibrio alginolyticus ...201424906123
comparative study of the physicochemical, compositional and functional properties of eight flours obtained from different plant materials found in colombia.the physicochemical, compositional and functional properties of flour from green plantains (musa acuminata) of the large green plantain variety, oyster mushrooms (pleorotus ostreatus), pineapple peel (ananas comosus) of the 'apple pineapple' variety, yellow peas (pisum sativum), chickpeas (cicer arietinum), whole grain rice (oryza sativa), whole grain corn (zea mays) and whole grain white quinoa (chenopodium quinoa) were evaluated by using one-way analysis of variance, pearson correlations and p ...201627091150
lectins with anti-hiv activity: a review.lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-hiv activity. the anti-hiv plant lectins include artocarpus heterophyllus (jacalin) lectin, concanavalin a, galanthus nivalis (snowdrop) agglutinin-related lectins, musa acuminata (banana) lectin, myrianthus holstii lectin, narcissus pseudonarcissus lectin, and urtica diocia agglutinin. the anti-hiv algal ...201525569520
soluble and wall-bound phenolics and phenolic polymers in musa acuminata roots exposed to elicitors from fusarium oxysporum f.sp. cubense.the accumulation of soluble and wall-bound phenolics and phenolic polymers in musa acuminata roots exposed to cell wall-derived elicitor from the pathogen, fusarium oxysporum, f.sp. cubense, race four, was investigated. the root tissue from the banana cultivar "goldfinger" was found to respond strongly and rapidly towards the elicitor through the increased synthesis of phenolic compounds. following elicitation, the conjugated and non-conjugated phenolic metabolites in the induced root tissue wer ...200312842140
production of bioethanol using agricultural waste: banana pseudo stem.india is amongst the largest banana (musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. two fungal strains aspergillus ellipticus and aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse wer ...201425477922
heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response.banana (musa acuminata) is one of the most popular fresh fruits. however, the rapid spread of fungal pathogen fusarium oxysporum f. sp. cubense (foc) in tropical areas severely affected banana growth and production. thus, it is very important to identify candidate genes involved in banana response to abiotic stress and pathogen infection, as well as the molecular mechanism and possible utilization for genetic breeding. heat stress transcription factors (hsfs) are widely known for their common in ...201627857174
identification, transcriptional and functional analysis of heat-shock protein 90s in banana (musa acuminata l.) highlight their novel role in melatonin-mediated plant response to fusarium one popular fresh fruit, banana (musa acuminata) is cultivated in the world's subtropical and tropical areas. in recent years, pathogen fusarium oxysporum f. sp. cubense (foc) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. however, the molecular mechanism of banana response to foc remains unclear, and functional identification of disease-related genes is also very limited. in this study, nine 90 kda heat-shock proteins (hsp90s) were genomewide i ...201727627033
activation of salicylic acid metabolism and signal transduction can enhance resistance to fusarium wilt in banana (musa acuminata l. aaa group, cv. cavendish).fusarium wilt caused by the fungus fusarium oxysporum f. sp. cubens (foc) is the most serious disease that attacks banana plants. salicylic acid (sa) can play a key role in plant-microbe interactions. our study is the first to examine the role of sa in conferring resistance to foc tr4 in banana (musa acuminata l. aaa group, cv. cavendish), which is the greatest commercial importance cultivar in musa. we used quantitative real-time reverse polymerase chain reaction (qrt-pcr) to analyze the expres ...201525277445
infestation of raoiella indica hirst (trombidiformes: tenuipalpidae) on host plants of high socio-economic importance for tropical america.the mite raoiella indica hirst was recently introduced into america, where it has shown amazing ability to disseminate and broaden its range of hosts. an experiment was conducted in cancún, mexico, to determine infestation levels of this mite on plants recorded as hosts: coconut palm (cocos nucifera) of cultivars pacific tall and malayan dwarf, oil palm (elaeis guineensis) hybrids deli x ghana and deli x nigeria, dwarf giant banana (musa acuminata, aaa subgroup cavendish), horn plantain (m. acum ...201626874954
sectional relationships in the genus musa l. inferred from the pcr-rflp of organelle dna sequences.the objective of this study was to construct a molecular phylogeny of the genus musa using restriction-site polymorphisms of the chloroplast (cpdna) and mitochondrial dna (mtdna). six cpdna and two mtdna sequences were amplified individually in polymerase chain reaction (pcr) experiments in 13 species representing the four sections of musa. ensete ventricosum (w.) ch. was used as the outgroup. the amplified products were digested with ten restriction endonucleases. a total of 79 restriction-site ...200312827254
analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of fusarium oxysporum f. sp. cubense.cavendish, the most widely grown banana cultivar, is relatively resistant to race 1 of fusarium oxysporum f. sp. cubense (foc1) which caused widespread panama disease during the first half of the 20th century but is susceptible to tropical race 4 of foc (foc tr4) which is threatening world banana production. the genome of the diploid species musa acuminata which is the ancestor of a majority of triploid banana cultivars has recently been sequenced. availability of banana transcriptomes will be h ...201324304681
molecular cloning and expression of five glutathione s-transferase (gst) genes from banana (musa acuminata l. aaa group, cv. cavendish).three tau class magsts responded to abiotic stress, magstf1 and magstl1 responded to signaling molecules, they may play an important role in the growth of banana plantlet. glutathione s-transferases (gst) are multifunctional detoxification enzymes that participate in a variety of cellular processes, including stress responses. in this study, we report the molecular characteristics of five gst genes (magstu1, magstu2, magstu3, magstf1 and magstl1) cloned from banana (musa acuminate l. aaa group, ...201323652818
cloning, bioinformatics and the enzyme activity analyses of a phenylalanine ammonia-lyase gene involved in dragon's blood biosynthesis in dracaena cambodiana.phenylalanine ammonia-lyase (pal) is the key enzyme of the phenylpropanoid pathway, playing an important role in plant development and defence. we cloned a partial cdna of pal gene, dcpal1, from dracaena cambodiana seedlings using rt-pcr with degenerate primers that were designed based on a multiple sequence alignment of known pal genes from other plant species. dcpal1 shows highly homologous to other known pal genes registered in genbank, being closest to that of musa acuminata. dcpal1 has a re ...201323108992
nuclear dna content and base composition in 28 taxa of musa.the nuclear dna content of 28 taxa of musa was assessed by flow cytometry, using line pxpc6 of petunia hybrida as an internal standard. the 2c dna value of musa balbisiana (bb genome) was 1.16 pg, whereas musa acuminata (aa genome) had an average 2c dna value of 1.27 pg, with a difference of 11% between its subspecies. the two haploid (ic) genomes, a and b, comprising most of the edible bananas, are therefore of similar size, 0.63 pg (610 million bp) and 0.58 pg (560 million bp), respectively. t ...200111550896
role of mediterranean diet, tropical vegetables rich in antioxidants, and sunlight exposure in blindness, cataract and glaucoma among african type 2 assess whether regular mediterranean diet and regular intake of vegetables may reduce the risk of blindness, cataract, and glaucoma in these type 2 diabetics.201222762057
the banana fruit dof transcription factor madof23 acts as a repressor and interacts with maerf9 in regulating ripening-related genes.the dna binding with one finger (dof) proteins, a family of plant-specific transcription factors, are involved in a variety of plant biological processes. however, little information is available on their involvement in fruit ripening. we have characterized 25 madof genes from banana fruit (musa acuminata), designated as madof1-madof25 gene expression analysis in fruit subjected to different ripening conditions revealed that madofs were differentially expressed during different stages of ripenin ...201626889012
genome-wide survey of the seagrass zostera muelleri suggests modification of the ethylene signalling network.seagrasses are flowering plants which grow fully submerged in the marine environment. they have evolved a range of adaptations to environmental challenges including light attenuation through water, the physical stress of wave action and tidal currents, high concentrations of salt, oxygen deficiency in marine sediment, and water-borne pollination. although, seagrasses are a key stone species of the costal ecosystems, many questions regarding seagrass biology and evolution remain unanswered. genom ...201525563969
telomere-centric genome repatterning determines recurring chromosome number reductions during the evolution of eukaryotes.whole-genome duplication (wgd) is central to the evolution of many eukaryotic genomes, in particular rendering angiosperm (flowering plant) genomes much less stable than those of animals. following repeated duplication/triplication(s), angiosperm chromosome numbers have usually been restored to a narrow range, as one element in a 'diploidization' process that re-establishes diploid heredity. in several angiosperms affected by wgd, we show that chromosome number reduction (cnr) is best explained ...201525138576
the interaction of banana mads-box protein mumads1 and ubiquitin-activating enzyme e-muuba in post-harvest banana fruit.key message : the interaction of mumads1 and muuba in banana was reported, which will help us to understand the mechanism of the mads-box gene in regulating banana fruit development and ripening. the ubiquitin-activating enzyme e1 gene fragment muuba was obtained from banana (musa acuminata fruit by the yeast two-hybrid method using the banana mads-box gene mumads1 as bait and 2-day post-harvest banana fruit cdna library as prey. mumads1 interacted with muuba. the interaction of mumads1 a ...201323007689
mechanisms of haplotype divergence at the rga08 nucleotide-binding leucine-rich repeat gene locus in wild banana (musa balbisiana).comparative sequence analysis of complex loci such as resistance gene analog clusters allows estimating the degree of sequence conservation and mechanisms of divergence at the intraspecies level. in banana (musa sp.), two diploid wild species musa acuminata (a genome) and musa balbisiana (b genome) contribute to the polyploid genome of many cultivars. the m. balbisiana species is associated with vigour and tolerance to pests and disease and little is known on the genome structure and haplotype d ...201020637079
high efficiency transformation of banana [musa acuminata l. cv. matti (aa)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.bananas and plantains (musa spp. l.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. here, we report efficient, rapid, and reproducible agrobacterium-mediated transformation and regeneration of an indian niche cultivar of banana [m. acuminata cv. matti (aa)]. apical meristem-derived highly proliferative multiple shoot clump (msc) explants were transformed with the agrobacterium strai ...201525173686
integrated syntenic and phylogenomic analyses reveal an ancient genome duplication in monocots.unraveling widespread polyploidy events throughout plant evolution is a necessity for inferring the impacts of whole-genome duplication (wgd) on speciation, functional innovations, and to guide identification of true orthologs in divergent taxa. here, we employed an integrated syntenic and phylogenomic analyses to reveal an ancient wgd that shaped the genomes of all commelinid monocots, including grasses, bromeliads, bananas (musa acuminata), ginger, palms, and other plants of fundamental, agric ...201425082857
gene expression analysis in musa acuminata during compatible interactions with meloidogyne incognita.endoparasitic root-knot nematodes (rkns) (meloidogyne spp.) cause considerable losses in banana (musa spp.), with meloidogyne incognita a predominant species in cavendish sub-group bananas. this study investigates the root transcriptome in musa acuminata genotypes 4297-06 (aa) and cavendish grande naine (cav; aaa) during early compatible interactions with m. incognita methods: roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological ...201728130221
curative and residual efficacy of injection applications of avermectins for control of plant-parasitic nematodes on banana.studies were conducted to determine the curative and residual efficacy of avermectins at controlling plant-parasitic nematodes when injected into the pseudostem of banana, musa acuminata cv. cavendish. in addition, we determined the lowest concentration of avermectins that provided satisfactory efficacy as protectants when injected into banana pseudostems. experiments were conducted with a root-knot nematode, meleidogyne javanica, and the burrowing nematode, radopholus similis. injections (1 ml) ...199719274271
extraction and partial characterization of polyphenol oxidase from banana (musa acuminata grande naine) roots.polyphenol oxidase activity (ppo, ec, monophenol monooxygenase, and ec, o-diphenoloxidase) has been extensively studied in banana fruit for its role in enzymatic browning. rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts indicate that ppo and phenolic compounds are ubiquitous in vegetative tissue of banana as well. they hamper biochemical and molecular studies in banana, as cumbersome adaptations of extraction protocols ...201616814556
monoxenic culture of banana-parasitic nematodes on musa acuminata cv. poyo shoots. 199019287766
genomic analysis of nac transcription factors in banana (musa acuminata) and definition of nac orthologous groups for monocots and dicots.identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. a comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. nac transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. the main goal of this study was to set up a framework of orthologous groups determined by an expert sequence compar ...201424570169
characteristics of three thioredoxin genes and their role in chilling tolerance of harvested banana fruit.thioredoxins (trxs) are small proteins with a conserved redox active site wcgpc and are involved in a wide range of cellular redox processes. however, little information on the role of trx in regulating low-temperature stress of harvested fruit is available. in this study, three full-length trx cdnas, designated matrx6, matrx9 and matrx12, were cloned from banana (musa acuminata) fruit. phylogenetic analysis and protein sequence alignments showed that matrx6 was grouped to h2 type with a typical ...201627618038
banana ovate family protein maofp1 and mads-box protein mumads1 antagonistically regulated banana fruit ripening.the ovate family protein named maofp1 was identified in banana (musa acuminata fruit by a yeast two-hybrid (y2h) method using the banana mads-box gene mumads1 as bait and a 2 day postharvest (dph) banana fruit cdna library as prey. the interaction between mumads1 and maofp1 was further confirmed by y2h and bimolecular fluorescence complementation (bifc) methods, which showed that the mumads1 k domain interacted with maofp1. real-time quantitative pcr evaluation of mumads1 and maofp1 expre ...201525886169
somatic embryogenesis and plants from immature zygotic embryos of the species musa acuminata and musa balbisiana.callus was obtained from immature zygotic embryos of semminiferous species (diploids) of musa sp. using a medium derived from that of murashige and skoog. picloram (7.5 μm) was added and the medium was solidified with gelrite (2 gl(-1)). differentiation of the first somatic embryos occurred after transfer of the callus in the presence of 7.5 μm picloram or 5.3 μm naa. somatic embryos germinated on the medium supplemented with 5.3 μm naa. serial sections of zygotic and somatic embryos showed perf ...198924240457
oral administration of banana lectin modulates cytokine profile and abundance of t-cell populations in mice.banana lectin (banlec) is a dimeric protein occurring in fruit pulp that modulates immune cell functioning in vitro. in order to assess the immune response in vivo, banlec from ripe banana (musa acuminata) fruit was purified and orally given to mice for seven days. the analysis of cytokines in the mice peripheral blood revealed increased il-10, il-17 and tnfα, and a reduction of ifnγ and il-6. in the thymus, an increase of cd4+ and a decrease of cd8+ t-cells were observed after oral administrati ...201627106589
genetic diversity and population structure of musa accessions in ex situ conservation.banana cultivars are mostly derived from hybridization between wild diploid subspecies of musa acuminata (a genome) and m. balbisiana (b genome), and they exhibit various levels of ploidy and genomic constitution. the embrapa ex situ musa collection contains over 220 accessions, of which only a few have been genetically characterized. knowledge regarding the genetic relationships and diversity between modern cultivars and wild relatives would assist in conservation and breeding strategies. our o ...201323497122
dietary intervention with green dwarf banana flour (musa sp aaa) prevents intestinal inflammation in a trinitrobenzenesulfonic acid model of rat colitis.dietary products are among the therapeutic approaches used to modify intestinal microflora and to promote protective effects during the intestinal inflammatory process. because the banana plant is rich in resistant starch, which is used by colonic microbiota for the anaerobic production of the short-chain fatty acids that serve as a major fuel source for colonocytes: first, green dwarf banana flour produces protective effects on the intestinal inflammation acting as a prebiotic and, second, comb ...201222464807
catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease.variations in cavendish bananas susceptibility to crown rot disease have been observed (lassois et al., 2010a), but the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. the present study was designed to compare gene expression between bananas (musa acuminata, aaa, 'grande-naine') showing a high post-harvest susceptibility (s+) and bananas showing a low post-harvest susceptibility (s-) to crown rot disease. this comparison was performed between cr ...201122702179
trace metals in vegetables and fruits cultivated around the surroundings of tummalapalle uranium mining site, andhra pradesh, india.vegetables (tomato - solanum lycopersicum, green chilli - capsicum annum and bitter gourd - momordica charantia) and fruits (banana - musa acuminata colla, papaya - carica papaya and mosambi - citrus limetta) from the cultivated areas around the tummalapalle uranium mining site were analyzed for trace metals (al, cr, mn, fe, ni, cu, zn, pb, be, v, co, cd and u) using inductively coupled plasma-mass spectrometer (icp-ms). as per the estimated data, the concentrations of trace metals in vegetables ...201428962264
evaluation of terrestrial plants extracts for uranium sorption and characterization of potent phytoconstituents.sorption capacity of four plants (funaria hygrometrica, musa acuminata, brassica juncea and helianthus annuus) extracts/fractions for uranium, a radionuclide was investigated by edxrf and tracer studies. the maximum sorption capacity, i.e., 100% (complete sorption) was observed in case of musa acuminata extract and fractions. carbohydrate, proteins, phenolics and flavonoids contents in the active fraction (having maximum sorption capacity) were also determined. further purification of the most a ...201625946322
field evidence of strong differential pollen placement by old world bat-pollinated plants.sympatric plant species that share pollinators potentially compete for pollination and risk interspecific pollen transfer, but this competition can be minimized when plant species place pollen on different areas of the pollinator's body. multiple studies have demonstrated strong differential pollen placement by sympatric plant species under laboratory conditions; however, field evidence collected in natural settings is less common. furthermore, it is unknown whether precise pollen placement on t ...201727864223
changes in the content and biosynthesis of phytoalexins in banana fruit.changes in the phytoalexin content in unripe fruit of banana, musa acuminata, were analyzed after various treatments. the results show that level of hydroxyanigorufone started to increase 1-2 day after either wounding or inoculation with conidia of colletotrichum musae. inoculation followed by wounding induced the formation of many other phenylphenalenones. the accumulation of hydroxyanigorufone decreased, after its transient maximum, on ripening by exposure of the wounded fruit to ethylene. the ...200011129580
synthesis of 2-(4-hydroxyphenyl)naphthalene-1,8-dicarboxylic anhydride, a phytoalexin isolated from unripe banana (musa acuminata).2-(4-hydroxyphenyl)naphthalene-1,8-dicarboxylic anhydride, a component of the phytoalexin that has been isolated from the peel of unripe banana (musa acuminata), was synthesized from 3-bromoacenaphthene.199926300176
differential pollen placement on an old world nectar bat increases pollination efficiency.plant species that share pollinators are potentially subject to non-adaptive interspecific pollen transfer, resulting in reduced reproductive success. mechanisms that increase pollination efficiency between conspecific individuals are therefore highly beneficial. many nocturnally flowering plant species in thailand are pollinated by the nectar bat eonycteris spelaea (pteropodidae). this study tested the hypothesis that plant species within a community reduce interspecific pollen movement by plac ...201626482654
a combined spectroscopic and tddft study of natural dyes extracted from fruit peels of citrus reticulata and musa acuminata for dye-sensitized solar cells.this study reports the novel spectroscopic investigations and enhanced the electron transfers of citrus reticulata and musa acuminata fruit peels as the photosensitizers for the dye-sensitized solar cells. the calculated td-dft-ub3lyp/6-31+g(d,p)-iefpcm(uaks), experiment spectra of ultra-violet-visible spectroscopy, and fourier transform infrared spectroscopy studies indicate the main flavonoid (hesperidin and gallocatechin) structures of the dye extracts. the optimized flavonoid structures are ...201727497289
phenylphenalenone-type phytoalexins from unripe buñgulan banana fruit.fourteen phenylphenalenone-type phytoalexins (1-14), including three new compounds, were isolated from the peel of unripe musa acuminata [aaa] cv. buñgulan fruit which had been injured and then inoculated with conidia of colletotrichum musae. these new phytoalexins were identified as (+)-cis-2,3-dihydro-2,3-dihydroxy-4-(4'-hydroxyphenyl)phenalen-1-one (12), 9-(3',4'-dimethoxyphenyl)-2-methoxyphenalen-1-one (13) and 9-(4'-hydroxyphenyl)-2-methoxyphenalen-1-one (14). the ratios of the relative int ...199827393357
computational identification and comparative analysis of mirna precursors in three palm the present study, mirna precursors in the genomes of three palm species were identified. analyzes of sequence conservation and biological function of their putative targets contribute to understand the roles of mirna in palm biology. micrornas are small rnas of 20-25 nucleotides in length, with important functions in the regulation of gene expression. recent genome sequencing of the palm species elaeis guineensis, elaeis oleifera and phoenix dactylifera have enabled the discovery of mirna ge ...201626919984
application of dyes extracted from alternanthera dentata leaves and musa acuminata bracts as natural sensitizers for dye-sensitized solar cells.the natural dyes anthocyanin and chlorophyll were extracted from musa acuminata bracts and alternanthera dentata leaves, respectively. the dyes were then applied as sensitizers in tio2-based dye-sensitized solar cells (dsscs). the ethanol extracts of the dyes had maximum absorbance. high dye yields were obtained under extraction temperatures of 70 to 80°c, and the optimal extraction temperature was approximately 80°c. moreover, dye concentration sharply decreased under extraction temperatures th ...201829133132
isolation and characterization of three cassava elongation factor 1 alpha (meef1a) plant genetic engineering, the identification of gene promoters leading to particular expression patterns is crucial for the development of new genetically modified plant generations. this research was conducted in order to isolate and characterize several new promoters from cassava (manihot esculenta crantz) elongation factor 1 alpha (ef1a) gene family.three promoters meef1a3, meef1a5 and meef1a6 were successfully isolated [corrected]. sequence analyses showed that all of the promoters conta ...201424404183
a tree of life based on ninety-eight expressed genes conserved across diverse eukaryotic species.rapid advances in dna sequencing technologies have resulted in the accumulation of large data sets in the public domain, facilitating comparative studies to provide novel insights into the evolution of life. phylogenetic studies across the eukaryotic taxa have been reported but on the basis of a limited number of genes. here we present a genome-wide analysis across different plant, fungal, protist, and animal species, with reference to the 36,002 expressed genes of the rice genome. our analysis ...201728922368
long noncoding rnas that respond to fusarium oxysporum infection in 'cavendish' banana (musa acuminata).long noncoding rnas (lncrnas) are a class of genes that influence a variety of biological functions through acting as signal, decoy, guide, and scaffold molecules. in banana (musa spp.), an important economic fruit crop, particularly in southeast asia, the wilt disease caused by fusarium oxysporum f. sp. cubense (foc), especially strain foc tr4, is disastrous. in banana, how the biogenesis of these lncrnas is regulated in response to pathogen infection is still largely unknown. in this study, st ...201729209086
the agpase family proteins in banana: genome-wide identification, phylogeny, and expression analyses reveal their involvement in the development, ripening, and abiotic/biotic stress responses.adp-glucose pyrophosphorylase (agpase) is the first rate-limiting enzyme in starch biosynthesis and plays crucial roles in multiple biological processes. despite its importance, agpase is poorly studied in starchy fruit crop banana (musa acuminata l.). in this study, eight maagpase genes have been identified genome-wide in m. acuminata, which could be clustered into the large (apl) and small (aps) subunits. comprehensive transcriptomic analysis revealed temporal and spatial expression variations ...201728757545
codon usage and codon pair patterns in non-grass monocot genomes.studies on codon usage in monocots have focused on grasses, and observed patterns of this taxon were generalized to all monocot species. here, non-grass monocot species were analysed to investigate the differences between grass and non-grass monocots.201729155926
the cloning of ty1-copia-like retrotransposons from 10 varieties of banana (musa sp.).ty1-copia-like retrotransposons have been identified and investigated in several plant species. here, the internal region of the reverse transcriptase (rt) gene of ty1-copia-like retrotransposons was amplified by pcr from total genomic dna of 10 varieties of banana. two to four clones from each variety were sequenced. extreme heterogeneity in the sequences of ty1-copia-like retrotransposons from all the varieties was revealed following sequence analysis of the reverse transcriptase (rt) fragment ...200212186754
dna profiling of banana and plantain cultivars using random amplified polymorphic dna (rapd) and restriction fragment length polymorphism (rflp) markers.polymerase chain reaction (pcr) amplification of genomic dna from 57 musa cultivars with 60 random 10-mer primers generated 605 polymorphic amplification products which were useful in unambiguous cultivar identifications. unweighted pair-group method analysis of this data grouped the cultivars into specific clusters depending on their genomic similarities. the diploid ancestral species of cultivated banana and plantains, namely musa acuminata sp malaccensis, an a genome donor and m. balbisiana, ...19958582364
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
phlebotomine sandfly ecology on the indian subcontinent: does village vegetation play a role in sandfly distribution in bihar, india?visceral leishmaniasis (vl) is a disease that results in approximately 50 000 human deaths annually. it is transmitted through the bites of phlebotomine sandflies and around two-thirds of cases occur on the indian subcontinent. indoor residual spraying (irs), the efficacy of which depends upon sandfly adults resting indoors, is the only sandfly control method used in india. recently, in bihar, india, considerable sandfly numbers have been recorded outdoors in village vegetation, which suggests t ...201728106262
xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east africa over the past decade.taxonomy: bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadales; family xanthomonadaceae; genus xanthomonas; currently classified as x. campestris pv. musacearum (xcm). however, fatty acid methyl esters analysis, genetic and genomic evidence suggest that this pathogen is x. vasicola and resides in a separate pathovar. isolation and detection: xcm can be isolated on yeast peptone glucose agar (ypga), cellobiose cephalexin agar and yeast tryptone sucrose agar (ytsa) com ...201728677256
the transcriptional regulatory network mediated by banana (musa acuminata) dehydration-responsive element binding (madreb) transcription factors in fruit ripening.fruit ripening is a complex, genetically programmed process involving the action of critical transcription factors (tfs). despite the established significance of dehydration-responsive element binding (dreb) tfs in plant abiotic stress responses, the involvement of drebs in fruit ripening is yet to be determined. here, we identified four genes encoding ripening-regulated dreb tfs in banana (musa acuminata), madreb1, madreb2, madreb3, and madreb4, and demonstrated that they play regulatory roles ...201728044313
two banana cultivars differ in composition of potentially immunomodulatory mannan and arabinogalactan.banana (musa acuminata and m. acuminata x m. balbisiana) fruit cell walls are rich in mannans, homogalacturonans and xylogalacturonan, rhamnogalacturonan-i, and arabinogalactans, certain forms of which is considered to have immunomodulatory activity. the cultivars nanicão and thap maeo represent two widely variants with respect to compositional differences in the forms of these polysaccharides. nanicão has low amounts of mannan in the water-insoluble and water-soluble fraction. both cultivars ha ...201728325331
antioxidant and antihyperglycemic properties of three banana cultivars (musa spp.).background. this study sought to investigate the antioxidant and antihyperglycemic properties of musa sapientum (latundan banana) (msl), musa acuminata (cavendish banana) (mac), and musa acuminate (red dacca) (mar). materials and methods. the sugar, starch, amylose, and amylopectin contents and glycemic index (gi) of the three banana cultivars were determined. furthermore, total phenol and vitamin c contents and α-amylase and α-glucosidase inhibitory effects of banana samples were also determine ...201627872791
structure characteristics of an acidic polysaccharide purified from banana (musa nana lour.) pulp and its enzymatic degradation.banana is one of the most important fruits over the world. the chemical composition is critical for the organoleptic properties and health benefits. as one of the leading bioactive components in banana pulp, the polysaccharides may contribute to the beneficial health effects. however, their precise structure information remains unknown. a leading acidic polysaccharide (abpp) of banana pulp was purified and identified by nuclear magnetic resonnance spectroscopy (nmr) and gas chromatography-mass s ...028336277
lineage-specific evolutionary histories and regulation of major starch metabolism genes during banana ripening.starch is the most widespread and abundant storage carbohydrate in plants. it is also a major feature of cultivated bananas as it accumulates to large amounts during banana fruit development before almost complete conversion to soluble sugars during ripening. little is known about the structure of major gene families involved in banana starch metabolism and their evolution compared to other species. to identify genes involved in banana starch metabolism and investigate their evolutionary history ...027994606
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