does kinin released by pineapple stem bromelain stimulate production of prostaglandin e1-like compounds? | | 1977 | 323193 |
bromelian, a thiolprotease from pineapple stem, depletes high molecular weight kininogen by activation of hageman factor (factor xiii). | | 1979 | 483261 |
allergic reactions, including asthma, to the pineapple protease bromelain following occupational exposure. | a 58-year-old pharmaceutical worker regularly developed asthma and rhinitis when handling bromelain, a purified protease of pineapple (ananas comosus), at her work-place, where she had been employed for about 10 years. rast and prick test showed strong positive reactions to bromelain. both inhalation test with 0.03 mg bromelain and peroral challenge by ingestion of 190 g pineapple resulted in asthmatic reactions; the latter challenge was accompanied by gastrointestinal symptoms. five of six work ... | 1979 | 498486 |
aflatoxigenic potential of dried figs, apricots, pineapples, and raisins. | spores from 3 aflatoxigenic strains were applied to the surface of dried figs, pineapple slices, apricots, and raisins. half of the samples of each foodstuff was autoclaved (cooked) before inoculation. all 3 strains produced some toxin but not in all of the foodstuffs. no aflatoxins were found in any of the raisin cultures. the overall potential for toxin production in the dried fruit was apricot greater than fig greater than pineapple greater than raisin. among the cooked substrates, the order ... | 1979 | 500544 |
purification and characterization of a proteinase from pineapple fruit, fruit bromelain fa2. | fruit bromelain fa2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized. 1. efficient extraction of this enzyme from the crude material was possible using "cellulosin ap," a microbial polysaccharidase preparation containing cellulase, hemicellulase, and pectinase. the enzyme was purified mainly by successive applications of anion-exchange chromatography, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunoc ... | 1976 | 956152 |
studies on the hydrazinolysis of glycoproteins. core structures of oligosaccharides obtained from porcine thyroglobulin and pineapple stem bromelain. | hydrazinolysis of porcine thyroglobulin glycopeptides and of pineapple stem bromelain [ec 3.4.22.4] permitted the isolation of almost intact carbohydrate chains of these glycoproteins. on the basis of permethylation analyses of the released oligosaccharides after reduction with nabh4, the core structures of unit a-type and unit b-type carbohydrate chains of porcine thyroglobulin were deduced to be manalpha1 leads to 6[manalpha1 leads to 3]manbeta1 leads to 4glcnacbeta1 leads to 4[ralpha1 leads t ... | 1976 | 1035216 |
mirex residues in wildlife and soils, hawaiian pineapple-growing areas--1972-74. | a monitoring program was conducted in the pineapple-growing areas of hawaii from 1972 to 1974 to survey mirex residues in sediments, soils, and aquatic and terrestrial wildlife. residues in pineapple field soils ranged from 3 to 18 ppb 9 months after mirex had been applied. no residues were found in the sediments. only 8 fish of 110 aquatic animals sampled contained mirex; these levels were low and ranged from 3 to 7 ppb. mirex residues in birds ranged from undetectable to 10 ppm; residues in ro ... | 1975 | 1221351 |
postprandial glucose and insulin responses to various tropical fruits of equivalent carbohydrate content in non-insulin-dependent diabetes mellitus. | the plasma glucose and insulin responses were determined in 10 niddm female patients following the ingestion of tropical fruit containing 25 g of carbohydrate. the five tropical fruits were pineapple, mango, banana, durian and rambutan. blood was drawn at 0, 30, 60, 120 and 180 min, respectively. the results showed that the glucose-response curves to mango and banana were significantly less than those to rambutan, durian and pineapple (p less than 0.05). only the glucose area after mango ingesti ... | 1991 | 1756683 |
[effect of non-enzymic glycosylation on reactivity in proteolysis]. | the nonenzymic glucation of most proteins occurs only at epsilon-amino groups of lysine residues. hydrolysis catalyzed by bovine trypsin, porcine trypsin and pineapple bromelian were studied using native and glucated proteins as substrates. glucosylated ovalbumin, human serum albumin, gamma-globulin and myoglobin show reduced susceptibility to degradation by trypsin as compared to the nonglucated proteins, apparently by direct modification of lysine residues. trypsin cleaves at substrate arginin ... | 1991 | 1843556 |
debridement of experimental full-thickness skin burns of rats with enzyme fractions derived from pineapple stem. | a limited in vivo study using 12 rats with full-thickness skin burns injuries was carried out. the animals were treated 24 h postburn with two newly discovered enzyme fractions derived from the stem of the pineapple (ananas comosus). the results indicated that even debridement of the injury could be effected rapidly (within 4 h). although the details of enzyme formulation and clinical application have yet to be established, these findings clearly suggest that two enzyme fractions from pineapple ... | 1990 | 2257064 |
the cysteine proteinases of the pineapple plant. | the pineapple plant (ananas comosus) was shown to contain at least four distinct cysteine proteinases, which were purified by a procedure involving active-site-directed affinity chromatography. the major proteinase present in extracts of plant stem was stem bromelain, whilst fruit bromelain was the major proteinase in the fruit. two additional cysteine proteinases were detected only in the stem: these were ananain and a previously undescribed enzyme that we have called comosain. stem bromelain, ... | 1990 | 2327970 |
[antimutagenic activity of vegetable and fruit extracts against in-vitro benzo(a)pyrene]. | about 80% of the juices from twenty vegetables and fruits showed antimutagenic activity when tested in the presence of the mutagen and carcinogen benzo[a]pyrene using the ames' salmonella/microsome assay. in a standardized test system juices from raw celeriac, broccoli, red cabbage, carrots, green, peppers, lettuce, asparagus, apricots, red-currants, gooseberries, raspberries, and pineapple showed more than 50% inhibition. leek, kohlrabi, cucumber, zucchini, french beans, fennel leaves, rhubarb, ... | 1990 | 2336853 |
inhibitory activity of cranberry juice on adherence of type 1 and type p fimbriated escherichia coli to eucaryotic cells. | inhibition of bacterial adherence to bladder cells has been assumed to account for the beneficial action ascribed to cranberry juice and cranberry juice cocktail in the prevention of urinary tract infections (a. e. sobota, j. urol. 131:1013-1016, 1984). we have examined the effect of the cocktail and juice on the adherence of escherichia coli expressing surface lectins of defined sugar specificity to yeasts, tissue culture cells, erythrocytes, and mouse peritoneal macrophages. cranberry juice co ... | 1989 | 2653218 |
stem bromelain: amino acid sequence and implications for weak binding of cystatin. | the amino acid sequence of stem bromelain, the major cysteine proteinase from pineapple stem is described. it shows that the enzyme is a member of the papain superfamily of cysteine proteinases, but is not very closely related to any other known member of this group. the sequence shows mutation or deletion of several residues that have been conserved in cysteine proteinases examined previously, including asn-175 (papain). we suggest that some of these changes have the effect of altering the acti ... | 1989 | 2714443 |
atomic absorption spectrophotometric determination of tin in canned foods, using nitric acid-hydrochloric acid digestion and nitrous oxide-acetylene flame: collaborative study. | twenty-six collaborators participated in a study to evaluate an atomic absorption spectrophotometric (aas) method for the determination of tin in canned foods. the 5 foods evaluated were meat, pineapple juice, tomato paste, evaporated milk, and green beans, each spiked at 2 levels. the concentration range of tin in the samples was 10-450 micrograms/g, and each level was sent as a blind duplicate. statistical treatment of results revealed no laboratory outliers and 6 individual or replicate-total ... | 1985 | 2985534 |
possible involvement of eicosanoids in the pharmacological action of bromelain. | bromelain, a proteolytic enzyme extracted from pineapple plants, was investigated for its capacity to interfere with arachidonic acid metabolism, since prostaglandins and other eicosanoids are well-known to be involved in the pathogenesis of inflammatory diseases. bromelain was tested for its ability to interfere with eicosanoids generation in vivo in two experimentally-induced inflammatory reactions in the rat. also antiplatelet aggregation activity of bromelain was studied in ex vivo rat plate ... | 1986 | 3006709 |
ananain: a novel cysteine proteinase found in pineapple stem. | a previously unknown cysteine proteinase, named ananain, has been isolated from crude commercial pineapple stem bromelain. the purification procedure involved affinity chromatography on sepharose-gly-phe-glycinaldehyde semicarbazone, and cation-exchange chromatography. the relative molecular mass of ananain was very similar to that of bromelain (25,000 and 26,000, respectively), but ananain differed greatly in specificity for hydrolysis of peptide and protein substrates. the new enzyme behaved a ... | 1988 | 3196029 |
enzymatic frostbite eschar debridement by bromelain. | bromelain is a plant proteinase derived from the stem of the pineapple plant that has been used successfully to debride the eschar from third-degree burn injuries. its applicability to frostbite eschar removal was extrapolated and investigated. third-degree frostbite lesions were produced on swine using supercooled air as the freezing media, and the resulting eschars were treated with a bromelain preparation. in the initial trial, no debridement other than that of the superficial layers of the e ... | 1987 | 3307553 |
the effects of fruit juices and fruits on the absorption of iron from a rice meal. | the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ... | 1987 | 3593665 |
debridement of experimental skin burns of pigs with bromelain, a pineapple-stem enzyme. | | 1973 | 4355066 |
breeding of aedes aegypti and a. simpsoni under the escarpment of the tanzanian plateau. | villages under the escarpment of the tanzanian plateau were surveyed for breeding of aedes aegypti. in some places more than 27% of the water containers outside houses harboured a. aegypti larvae, while there was practically no breeding in containers inside houses. from 2% to 10% of tree holes contained a. aegypti larvae. in places, as many as 47 a. simpsoni larvae were collected from one pineapple plant, and the total mean number of larvae per pineapple was 6.6, while the percentage of plants w ... | 1972 | 4538907 |
pineapple - and -d-mannopyranosidases and their action on core glycopeptides. | | 1972 | 5030637 |
a study on the protective effect of bromelain, crude pineapple proteases, against adrenaline-induced pulmonary edema in rats. | | 1967 | 5300570 |
antifertility screening of plants. vi. effect of five indigenous plants on early pregnancy in albino rats. | the petroleum ether, alcoholic and aqueous extracts of apium graveolens linn., butea monosperma lam. kuntz., and gossypium herbaceum linn., the aqueous extract of aloe barbadensis mill.syn., and the juice of unripe fruits of ananas comosus were tested on albino rats by a method which detects any antizygotic, blastocystotoxic, antiimplantation, and early abortifacient activity. the extracts were administered for 1-7 days. the dosages for a. graveolens, b. monosperma, and g. herbaceum were 100 ... | 1970 | 5505214 |
effect of feeding limited roughage and a comparison between loose and pelleted pineapple hay on milk production, milk constituents, and fatty acid composition of milk fat. | | 1966 | 5908799 |
feeding of irradiated chicken, beef, and pineapple jam to dogs. | | 1966 | 5956876 |
fibrinolytic and antithrombotic action of bromelain may eliminate thrombosis in heart patients. | it has been established that a bromelain plasminogen activator will produce plasmin in rat experiments. in addition the plasmin cleaves hageman factor in a way that leads to a strong release of kallikrein but a weak release of thrombin. a possible mechanism is suggested to explain how the body can maintain thrombin at a level too low to cause platelet aggregation but adequate to stimulate release of prostaglandins and enzymes for more than 24 hours from a single dose of the pineapple enzymes. si ... | 1980 | 6256612 |
tin in foods and the diet. | the diet is the main source of tin intake by man. nearly all the tin in the uk diet is associated with canned food. canned tomatoes, tomato products, pineapple, pears and similar fruits contain the highest concentrations of tin. average dietary tin intakes in the uk are about 3 mg/day and have been falling since 1976. | 1984 | 6537352 |
mutagenicity, carcinogenicity and reproductive effects of dibromochloropropane (dbcp). | dibromochloropropane (dbcp), a brominated organochlorine nematocide, has been used since the mid-1950s. its primary value was its effectiveness on perennial crops without damaging the plants. dbcp also had less acute toxicity than earlier soil fumigants such as ethylene dibromide. dbcp became widely used on citrus, grapes, peaches, pineapple, soybeans and tomatoes. in central america and israel, banana trees were treated with dbcp. by 1975, 25 million pounds were being produced in the u.s. per y ... | 1983 | 6888412 |
study of lung flukes from philippines: --a preliminary report. | in this paper taxonomic study on paragonimus including their metacercariae obtained from the crabs collected from sorsogon, luzon, philippines and adult worms and ova obtained through animal feeding, with light microscopy and scanning electron microscopy revealed that there may exist two or more species of lung flukes in philippines. the occurrence of semisulcospira libertina in addition to antemelania dactylus (lea) as an additional snail host is mentioned for the first time. the chromosome kar ... | 1982 | 7100903 |
chemical debridement of burns: mercaptans. | experiments were conducted using non-enzymatic chemical agents (with emphasis on certain mercaptans), alone, in conjunction with enzymatic agents and/or other nonenzymatic chemicals for debridement of burns. both in vitro (rats, pigs, humans) and in vivo (rats, pigs) tests were carried out. n-acetylcysteine, penicillamine and cysteine ethyl ester in low to moderate concentrations accelerate the debriding action of bromelain (an enzymatic preparation from pineapple stems) and in higher concentrat ... | 1981 | 7265335 |
nutritional requirements and biochemical activities of pineapple pink disease bacterial strains from hawaii. | bacteria which cause pink disease of pineapple, identified on the basis of their nutritional and biochemical activities, were found to belong to three genera. these bacteria include the following species: gluconobacter oxydans, acetobacter aceti, and erwinia herbicola. several pink disease strains required one to three vitamins for growth. both g. oxydans strains 303d and 180 required biotin, nicotinic acid, and pantothenic acid for growth; e. herbicola 189 required only nicotinic acid; however, ... | 1980 | 7436404 |
kinetic comparison of peptide: n-glycosidases f and a reveals several differences in substrate specificity. | the initial velocities of hydrolysis of nineteen glycopeptides by peptide: n-glycosidase f and a were determined. substrates were prepared from bovine fetuin, hen ovalbumin, pineapple stem bromelain, bovine fibrin and taka-amylase. from these glycopeptides, several variants with regard to peptide and carbohydrate structure were prepared and derivatized with dabsyl chloride, dansyl chloride or activated resorufin. tyrosine containing glycopeptides were also used without an additional chromophore. ... | 1995 | 7540902 |
foodborne disease outbreak due to consumption of rancid biscuits. | a foodborne disease outbreak characterized by vomiting, abdominal pain and diarrhea, involving mostly children was investigated. epidemiological, hospital and laboratory investigations indicated that the disease outbreak was associated with consumption of rancid biscuits abandoned in the street corner in a crowded locality of old city hyderabad. the offensive flavors of rancidity were masked by the strong pineapple flavor used in the biscuits. rancidity of the biscuits was confirmed by high pero ... | 1995 | 7760445 |
conformational analysis of the xylose-containing n-glycan of pineapple stem bromelain as part of the intact glycoprotein. | the conformational behavior of the n-glycan man alpha 1-6(xyl beta 1-2)man beta 1-4glcnac beta 1-4(fuc alpha 1-3)glcnac beta of stem bromelain as part of the intact glycoprotein was investigated and compared with that of the same n-glycan as part of a bromelain-derived glycopeptide. proton chemical shifts of the glycoprotein n-glycan were determined by 2d hohaha and 2d noesy measurements, making use of the glycopeptide 1h nmr data. during each 2d nmr experiment about 4% of the glycoprotein denat ... | 1995 | 7794934 |
trypanosoma brucei, t. congolense and t. vivax infections in horses on a farm in kenya. | equines are particularly susceptible to infection with trypanosoma evansi and t. brucei, but rarely is natural t. congolense and t. vivax infection seen in horses. an outbreak of trypanosomosis occurred in a herd of horses used for patrolling the pineapple fields on the del monte farm, thika, kenya initially involving 6 horses. on subsequent screening of the entire group, t. brucei, t. congolense and t. vivax infections were detected in 16 of the 35 horses. the tests used for diagnosis included ... | 1994 | 7941037 |
kiwi fruit allergy: a new birch pollen-associated food allergy. | to determine the cross-reacting antigens of kiwi fruit and other foods and pollen, we investigated 22 patients allergic to kiwi fruit: 10 with severe systemic reactions and 12 with localized symptoms confined to oral and pharyngeal mucosa (oral allergy syndrome). seven patients with birch pollen allergy who tolerated kiwi fruit were included as a control group. | 1994 | 8027500 |
resistance to heavy metals by some nigerian yeast strains. | the heavy metal resistance of yeasts isolated from sugary substrates such as orange, palm wine and pineapple and identified as saccharomyces carlsbergensis and s. cerevisiae was studied. the yeast isolates were tested against different concentrations of cadmium, copper, manganese, silver and zinc salts ranging from 1 to 20 mmol/l. local yeasts showed resistance to 3-15 mmol/l cadmium, 18-20 copper, 16-20 manganese, 1-9 silver and 16-19 for zinc. the significance of the results is discussed in re ... | 1993 | 8112693 |
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. | the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ... | 1994 | 8206443 |
inductively coupled plasma atomic emission spectrometric determination of tin in canned food. | various canned foods were digested sequentially with hno3 and hcl, diluted to 100 ml, and filtered, and then tin was determined by inductively coupled plasma atomic emission spectrometry (icp/aes). samples of canned satsuma mandarin, peach, apricot, pineapple, apple juice, mushroom, asparagus, evaporated milk, short-necked clam, spinach, whole tomato, meat, and salmon were evaluated. sample preparations did not require time-consuming dilutions, because icp/aes has wide dynamic range. the standar ... | 1993 | 8286977 |
oral administration of protease inhibits enterotoxigenic escherichia coli receptor activity in piglet small intestine. | the virulence of enterotoxigenic escherichia coli (etec) is attributed to their ability to adhere via fimbrial adhesins to specific receptors located on the intestinal mucosa. a novel approach to preventing etec induced diarrhoea would be to prevent attachment of etec to intestine by proteolytically modifying the receptor attachment sites. this study aimed to examine the effect of bromelain, a proteolytic extract obtained from pineapple stems, on etec receptor activity in porcine small intestine ... | 1996 | 8566855 |
outbreak of bronchiolitis obliterans associated with consumption of sauropus androgynus in taiwan. | in august, 1995, there was an outbreak in taiwan of rapidly progressive respiratory distress associated with consumption of uncooked sauropus androgynus, a vegetable with a claimed yet unconfirmed effect on weight control. we report on 23 patients with strikingly similar clinical presentations. | 1996 | 8676721 |
[microbiological quality of street sold fruits in san josé, costa rica]. | the sanitary quality of street sold fruits was analyzed during the period from march 1990 thru march 1993 in san jose, costa rica. it looked for the presence of salmonella spp. shigella spp., escherichia coli as well as fecal coliforms in natural refreshments, fruit salads and the fruits most frecuently expended on streets, either in slices as the pineapple (ananas comosus), papaya (carica papaya), non-ripe mangoe (mangifera indica) and watermelon (citrullus vulgaris) and those that can be eaten ... | 1995 | 8729262 |
[evaluation of biological fish silage in broiler chicken]. | biological fish silage was produced from the mixture of three low price fish species. after mixing it with molasse, papaya and pineapple wastes, and lactobacillus plantarum incoculation, the product was stored for seven days, dehydrated and added to several diets of formulated poultry feed as a substitute of fish meal. physical-chemical and microbiological analyses were done to the raw materials and final products. biological tests of acceptability indicated that chicken preferred diets up to 50 ... | 1995 | 8729265 |
[microbial silage production from eviscerated fish]. | microbial fish silage was produced from a mixture of several fish species that belong to the shrimp by-catch. they were mixed with molasses, fruits (pineapple and papaya), sorbate and a starter of lactobacillus plantarum atcc8014. process was evaluated by ph, acidity, consistency, exudate liquid, non-protein nitrogen, total volatile bases, microbial and toxicological tests. results indicated that acid production and ph reduction occurs during the first two days of processing, later these values ... | 1993 | 8779624 |
identification and characterization of a pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple. | pantoea citrea, a member of the family enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. molecular genetic approaches were applied to elucidate the mechanism responsible for this fruit discoloration. a p. citrea mutant strain, cmc6, defective in its ability to cause pink disease and fruit discoloration, was generated by nitrosoguanidine mutage ... | 1997 | 8979341 |
effect of plant extracts and systemic fungicide on the pineapple fruit-rotting fungus, ceratocystis paradoxa. | antifungal activities of extracts of sixteen plants were tested against ceratocystis paradoxa which causes soft rot of pineapples. xanthium strumarium was the most effective followed by allium sativum. the effectiveness of various extracts against c. paradoxa was in the decreasing order of meriandra bengalensis, mentha piperita, curcuma longa, phlogacanthus thyrsiflorus, toona ciliata, vitex negundo, azadirachta indica, eupatorium birmanicum, ocimum sanctum and leucas aspera. extracts of cassia ... | 1996 | 9022263 |
immunological cross-reactivity of the major allergen from perennial ryegrass (lolium perenne), lol p i, and the cysteine proteinase, bromelain. | antibodies prepared in rabbits against the major allergen from ryegrass (lolium perenne), lol p i, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. the results indicate that the carbohydrate residues from these allergens play an important role in ... | 1997 | 9104799 |
bromelain prevents secretion caused by vibrio cholerae and escherichia coli enterotoxins in rabbit ileum in vitro. | diarrhea is a major cause of illness and death in children and young animals. the aim of this study was to investigate the possible therapeutic effect of bromelain, a proteolytic extract obtained from pineapple stems on bacterial toxin and second-messenger agonist-induced intestinal secretion. | 1997 | 9207276 |
inhibition of the mutagenicity of 2-nitrofluorene, 3-nitrofluoranthene and 1-nitropyrene by vitamins, porphyrins and related compounds, and vegetable and fruit juices and solvent extracts. | when 21 vitamins including related compounds haemin, chlorophyllin, chlorophyll, biliverdin and bilirubin, as well as juices from five fruits and 25 vegetables and solvent extracts from the residues of fruits and vegetables were tested for their antimutagenic potencies with respect to mutagenicity induced by 2-nitrofluorene (2-nf), 3-nitrofluoranthene (3-nfa) and 1-nitropyrene(1-np) in salmonella typhimurium ta98 the following results were obtained. the tetracyclic nitroarenes 3-nfa and 1-np wer ... | 1997 | 9207899 |
intestinal absorption of undegraded proteins in men: presence of bromelain in plasma after oral intake. | the human adult intestinal epithelium has traditionally been described as nonpermeable to proteins. however, indirect evidence suggests that reduced absorption of undegraded proteins might take place under physiological conditions. using bromelain (an enzyme obtained from pineapple stems) as a model protein, we studied the extent of this mucosal permeation in 19 healthy men. the protein was detected in plasma by immunoassay and by its proteolytic activity after oral administration. the estimated ... | 1997 | 9252520 |
recent case histories of food product-metal container interactions using scanning electron microscopy-x-ray microanalysis. | scanning electron microscopy (sem) and x-ray microanalysis (eds) were used to investigate stress corrosion cracking (scc) in plain tinplate food cans, product discoloration, filiform corrosion, pitting/perforation corrosion, and loss of coating adhesion in enameled food cans. intergranular scc of uncoated tinplate occurred in canned pineapple juice. this is a rare occurrence in acid food products. the fracture developed in the can headspace only at the product line/metal interface by an interact ... | 1997 | 9353869 |
complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. | the amino acid sequences of ananain (ec3.4.22.31) and stem bromelain (3.4.22.32), two cysteine proteases from pineapple stem, are similar yet ananain and stem bromelain possess distinct specificities towards synthetic peptide substrates and different reactivities towards the cysteine protease inhibitors e-64 and chicken egg white cystatin. we present here the complete amino acid sequence of ananain and compare it with the reported sequences of pineapple stem bromelain, papain and chymopapain fro ... | 1997 | 9355753 |
shelf life study of some nigerian fruit juices inoculated with ascospores of neosartorya spp. | mango, orange and pineapple juices containing various concentrations of sucrose were dispensed in 100 ml volumes into 100 ml conical flasks with stoppers. each juice in a flask was inoculated with ascospores of one local isolate of either neosartorya fischeri, n. fischeri var. spinosa or n. quadricincta to a final concentration of 120-140 ascospores/ml of juice, and then pasteurized at 80 degrees c for 30 min. a set of 36 inoculated flasks with 12 controls was stored at 4-5 degrees c and two set ... | 1997 | 9477427 |
a shipyard outbreak of salmonellosis traced to contaminated fruits and vegetables. | a large outbreak of food poisoning occurred in september 1996 and involved at least 116 workers at a shipyard in jurong. salmonella weltevreden was isolated from the stool specimens of 24 hospitalised cases and three food handlers, giving a total of 27 bacteriologically confirmed cases (25 symptomatic and two asymptomatic) in this outbreak. based on a case-control analysis, a number of food items consumed on 23 and 24 september 1996 were implicated in the outbreak, viz. a vegetable dish (p < 0.0 ... | 1997 | 9494655 |
isolation and characterization of two forms of an acidic bromelain stem proteinase. | two forms of an acidic bromelain proteinase isolated from crude bromelain, an extract from pineapple stem, were found by a two-step fplc purification procedure. the basic main components were removed by cation exchange chromatography and the breakthrough fraction was further resolved by anion exchange chromatography into 15 protein fractions, only two of which, called sba/a and sba/b, were proteolytically active. these components were characterized by electrospray mass spectroscopy (esms), isoel ... | 1998 | 9619588 |
evaluation of growth of neosartorya spp. on some nigerian fruit products. | growth of neosartorya fischeri, n. fischeri var. spinosa and n. quadricincta on inoculated fruit juice agars (fjas) consisting of 1% agar and juices from mango (mja), orange (oja) and pineapple (pja) containing various levels of added sucrose was studied in terms of the average number of colonies produced by their ascospores, time (days) required before colonies were formed and developed to confluent mycelial mat as well as the level of reproductive spores produced. the means of the number of co ... | 1997 | 9650724 |
influence of growth medium on thermal resistance of pediococcus sp. nrrl b-2354 (formerly micrococcus freudenreichii) in liquid foods. | pediococcus sp. is a nonpathogenic heat-resistant spoilage organism that has been used as a test organism in milk pasteurization studies. these characteristics make this bacterium an attractive test organism to study the mode of bacterial thermal inactivation in a food pilot plant. we report here the effect of growth medium on the thermal d value of this organism in skim milk, whole liquid egg, 10% glucose solution, pineapple juice, apple juice, tomato juice, and water at 60 degrees c. thermal i ... | 1998 | 9709230 |
inactivation of microorganisms with microwaves at reduced temperatures. | we developed a pilot-plant nonthermal flow process using microwave energy to inactivate microorganisms. the process consists of multiple passes through the microwave generator. each passed material goes to a receiving tank for subsequent passes. the flow rate was 0.96 to 1.26 kg/min and the dwell time per pass was 1.1 to 1.5 min. five passes were used. the microwave energy is instantaneously and simultaneously applied to the system, and thermal energy is removed by a cooling tube within the proc ... | 1998 | 9709231 |
characterization of pucd5000 involved in pink disease color formation by pantoea citrea. | pantoea citrea, the causal agent of pink disease of pineapple, harbors a cryptic plasmid of 5229 bp. designated pucd5000. on the basis of nucleotide and amino acid sequence analyses, pucd5000 contains both replication and mobilization loci (bom and mobcabd) that are similar to those in plasmids psw100 and psw200 in pantoea stewartii and pec3 in erwinia carotovora subsp. carotovora. the survival of p. citrea on pineapple does not depend on pucd5000. however, full pink coloration development, whic ... | 1998 | 9735319 |
polymerase chain reaction for rapid detection of campylobacter jejuni in artificially contaminated foods. | campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. these food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (pcr). specific detection of camp. jejuni, as indicated by a pcr product of 159 bp, was possible with all pre-cooked deli-sliced and raw poultry products tested. all vegetables tested were compatible with the pcr assay. cantaloupe, kiwi and pineapple tested positive while strawberries, watermelon, grapes and ... | 1998 | 9750320 |
effect on cell surface hydrophobicity and susceptibility of helicobacter pylori to medicinal plant extracts. | effects on aqueous extracts of medicinal plants on ten helicobacter pylori strains were studied by the salt aggregation test to determine the possibility to modulate their cell surface hydrophobicity and by an agar diffusion assay for detection of antimicrobial activity. it was established that aqueous extracts of bearberry and cowberry leaves enhance cell aggregation of all h. pylori strains tested by the salt aggregation test, and the extract of bearberry possessed a remarkable bacteriostatic ... | 1999 | 10079525 |
bromelain protects piglets from diarrhoea caused by oral challenge with k88 positive enterotoxigenic escherichia coli. | k88 positive enterotoxigenic escherichia coli (k88+ etec) is an important cause of diarrhoea in young piglets. k88+ etec pathogenesis relies on attachment to specific glycoprotein receptors located on the intestinal mucosa. proteolytic treatment of these receptors in vitro and in vivo prevents attachment of k88+ etec to piglet small intestines and may be of clinical use to prevent k88+ etec pathogenesis. | 1998 | 10189844 |
bromelain proteases reduce human platelet aggregation in vitro, adhesion to bovine endothelial cells and thrombus formation in rat vessels in vivo. | the thiol protease, bromelain, an extract from pineapple stem, was suggested to have antithrombotic and anticoagulant activities in vivo. we studied the effects of bromelain on cell size distribution of isolated human platelets in vitro by coulter counter measurements. preincubation of platelets with bromelain (10 micrograms/ml) completely prevented the thrombin (0.2 u/ml) induced platelet aggregation. papain was less active in preventing platelet aggregation. in vitro, bromelain (0.1 microgram/ ... | 1999 | 10218125 |
gdhb, a gene encoding a second quinoprotein glucose dehydrogenase in pantoea citrea, is required for pink disease of pineapple. | the pink disease of pineapple, caused by the bacterium pantoea citrea, is characterized by a dark coloration on fruit slices after canning. a glucose dehydrogenase (gdh) encoded by the gdha gene has been implicated in the colour formation activity of p. citrea. in this paper it has been shown that p. citrea contains a second, homologous gdh gene and its product, gdhb, represents the main source of gdh activity in this organism. unlike gdha, gdhb is constitutively expressed during the exponential ... | 1999 | 10376838 |
differentiation of fusarium subglutinans f. sp. pini by histone gene sequence data. | fusarium subglutinans f. sp. pini (= f. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the gibberella fujikuroi species complex. this species complex includes f. thapsinum, f. moniliforme (= f. verticillioides), f. nygamai, and f. proliferatum, as well as f. subglutinans associated with sugarcane, maize, mango, and pineapple. differentiating these forms of f. subglutinans usually requires pathogenicity tests, which are often time-consuming ... | 1999 | 10427026 |
investigation of an outbreak of gastroenteritis at a hospital for patients with learning difficulties. | eighty of the 460 patients and staff (attack rate 22%) in a long-stay hospital for patients with learning difficulties became ill in a general outbreak of gastrointestinal infection that followed a buffet style party on one ward. illness in the cohort of 47 resident patients, relations, and staff who attended that party was associated with having eaten ham, coleslaw, bread rolls, and cheese and pineapple on sticks. food from the hospital kitchen was supplemented by food brought in and prepared o ... | 1999 | 10462893 |
the naturally occurring furanones: formation and function from pheromone to food. | three closely related 4-hydroxy-3(2h)-furanones have been found in a range of highly cooked foodstuffs where they are important flavour compounds with aroma threshold values as low as 20 micrograms kg-1 water (approximately 0.14 mumol l-1). the compounds are formed mainly as a result of the operation of the maillard reactions between sugars and amino acids during heating but one compound, 5-(or 2)-ethyl-2-(or 5)-methyl-4-hydroxy-3(2h)-furanone, appears in practice to be produced by yeast, probab ... | 1999 | 10466251 |
involvement of carbohydrate epitopes in the ige response of celery-allergic patients. | this study was performed to get further insights into antibody responses to cross-reactive carbohydrate determinants (ccd), including initial experiments to prove the biological activity of anti-ccd ige. earlier studies have shown that ige specific for ccd occurs in about 25% of celery-allergic patients. the clinical significance of these antibody specificities is doubtful. | 1999 | 10529586 |
antimutagenic effect of fruit and vegetable ethanolic extracts against n-nitrosamines evaluated by the ames test. | the inhibitory effect of nine fruit and vegetable ethanolic extracts against the mutagenicity of n-nitrosodimethylamine (ndma), n-nitrosopyrrolidine (npyr), n-nitrosodibutylamine (ndba), and n-nitrosopiperidine (npip) was evaluated by means of the ames test. licorice ethanolic extract was the only one that showed an inhibitory effect (ranging from moderate to strong) against mutagenicity of all n-nitrosamines tested. this ethanolic extract showed the greatest inhibition effect against npip (72%) ... | 1999 | 10552641 |
enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase. | sucrose phosphorylase from leuconostoc mesenteroides catalyzed transglucosylation from sucrose to 4-hydroxy-3(2h)-furanone derivatives. when 4-hydroxy-2,5-dimethyl-3(2h)-furanone (hdmf) and 2-ethyl-4-hydroxy-5-methyl-3(2h)-furanone or 5-ethyl-4-hydroxy-2-methyl-3(2h)-furanone (ehmf) were used as acceptors, their transfer ratios were more than 45%. in the case of glucosylation of hdmf, the major transfer product was identified as 2,5-dimethyl-3(2h)-furanone 4-o-alpha-d-glucopyranoside (dmf-g). in ... | 2000 | 10705458 |
genetic and biochemical characterization of the pathway in pantoea citrea leading to pink disease of pineapple. | pink disease of pineapple, caused by pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. this coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. to elucidate the biochemical pathway leading to pink disease, we generated six coloration-defective mutants of p. citrea that were still able to oxidize glucose into gluconate. three mutants were found to be af ... | 2000 | 10735866 |
natural endophytic occurrence of acetobacter diazotrophicus in pineapple plants. | the presence of endophytic acetobacter diazotrophicus was tested for pineapple plants (ananas comosus [l.] merr.) grown in the field. diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. phenotypic tests permitted the selection of presumptive nitrogen-fixing a. diazotrophicus isolates. restriction fragment length polymorphisms (rflps) of small subunit (ssu) rdna using total dna digested with endonuclease sphi and with endo ... | 2000 | 10790517 |
[assessment of the impact of the biological rodenticide biorat in populations of rodents settled in several crops of the republic of costa rica]. | the impact of biological rodenticide biorat on populations of rodents settled in some crops was determined; these crops are highly infested with sigmodon hispidus, a very common vector in various central american countries. the trapping technique and the appraisal of damages allowed to calculate the index of infestation which ranged from 75 to 90 percent in three crops. twenty for to 36 kg of biorat were applied to 6 ha planted with cucumis sativus (cucumber), ipomoea batata (sweet potato) and a ... | 1999 | 10887586 |
intestinal absorption of calcium from foodstuffs as compared to a pharmaceutical preparation. | only few data are available on intestinal calcium absorption from foodstuffs and composite meals in humans. the aim of the study was to compare intraindividually the calcium absorption from milk and from a breakfast with that from a pharmaceutical calcium preparation of equal calcium content. in 8 healthy volunteers between 44 and 58 years of age, the intestinal calcium absorption was measured in randomized order applying the double isotope technique from: (1) 500ml of fresh milk (equivalent to ... | 1999 | 10902536 |
continuous ethanol production from pineapple cannery waste using immobilized yeast cells. | the cells of saccharomyces cerevisiae atcc 24553, were immobilized in k-carrageenan and packed in a tapered glass column reactor for ethanol production from pineapple cannery waste at temperature 30 degrees c and ph 4.5. the maximum productivity was 42.8 g ethanol 1(-1) h(-1) at a dilution rate of 1.5 h(-1). the volumetric ethanol productivity of the immobilized cells was ca. 11.5 times higher than the free cells. the immobilized cell reactor was operated over a period of 87 days at a dilution r ... | 2000 | 10908799 |
dietary serotonin and alcohol combined may provoke adverse physiological symptoms due to 5-hydroxytryptophol. | the urinary excretion products of serotonin (5-hydroxytryptamine, 5ht) are 5-hydroxyindole-3-acetic acid (5hiaa) and 5-hydroxytryptophol (5htol), and the ratio of 5htol to 5hiaa is normally very low (< 0.01 ) in man. intake of foods rich in 5ht (high amounts in banana, pineapple, and walnuts) induces a general increase in the output of 5ht metabolites, without affecting the 5htol/5hiaa ratio. in contrast, during metabolism of ethanol there is a shift in the catabolic pattern of 5ht, and the form ... | 2000 | 10968409 |
mutagenesis and kinetic studies of a plant cysteine proteinase with an unusual arrangement of acidic amino acids in and around the active site. | we report the cloning, overexpression, kinetic analysis, and modeling of the tertiary structure of an unusual plant cysteine proteinase. ananain (ec 3.4.22.31), from ananas comosus (pineapple) is distinguished from all other cysteine proteinases in the papain superfamily by having a unique combination of acidic amino acids. as well as lacking the acidic residue immediately preceding the active site histidine (position 158 in papain), it also lacks the extensive surface network of acidic residues ... | 2000 | 10998237 |
[parasitic helminths from sigmodon hispidus (rodentia: cricetidae) from seasonal and evergreen habitats in costa rica]. | the helminthological fauna of the cotton rat, sigmodon hispidus in a tropical environment varies according to habitat and feeding behavior. six species of nematodes (longistriata adunca, trichostrongylus sigmodontis, strongyloides sigmodontis, litomosoides carinii, monodontus sp. and protospirura sp.) and two species of cestodes (hymenolepis diminuta and raillietina sp.) were found in rats from extensive dry lands in guanacaste where hot temperatures and heterogeneous diet are the rule. only two ... | 2000 | 11021315 |
an outbreak of campylobacter jejuni infections associated with food handler contamination: the use of pulsed-field gel electrophoresis. | in 1998, an outbreak of campylobacter jejuni infections occurred in kansas among persons attending a school luncheon; community cases were also reported. in a cohort study of luncheon attendees, 27 (17%) of 161 persons reported illness. consuming gravy (relative risk [rr], 4.2; 95% confidence interval [ci], 1.5-11.7) or pineapple (rr, 2.4; 95% ci, 1.0-5.7) was associated with illness. both foods were prepared in a kitchen that served 6 other schools where no illness was reported. a cafeteria wor ... | 2001 | 11078485 |
evaluation of residual levels of benomyl, methyl parathion, diuron, and vamidothion in pineapple pulp and bagasse (smooth cayenne). | the objective of this research was to study the residual levels of benomyl, methyl parathion, diuron, and vamidothion in pineapple bagasse and pulp. benomyl (benlate), methyl parathion (folidol 600), diuron (krovar), and vamidothion (kilval 300) were applied pre-harvest to pineapples (smooth cayenne). after harvesting, the fruits were washed (100 ppm sodium hypochlorite) and the pulp was separated from the sub-products (peel, core, tops, and tails). the pulp was not submitted to any heat treatme ... | 2000 | 11087549 |
nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2. | the genome of pineapple mealybug wilt-associated closterovirus-2 (pmwav-2) was cloned from double-stranded rna isolated from diseased pineapple and its sequence determined. the 3'-terminal 14861 nt of the single-stranded rna genome contains ten open reading frames (orfs) which, from 5' to 3', potentially encode a >204 kda polyprotein containing papain-like protease, methyltransferase and helicase domains (orf1a), a 65 kda rna-dependent rna polymerase (orf1b), a 5 kda hydrophobic protein (orf2), ... | 2001 | 11125151 |
role of yersinia intermedia and pseudomonas putida in the development of a fruity off-flavour in pasteurized milk. | analysis by gas chromatography-mass spectrometry of pasteurized milk with a fruity (pineapple like) off odour and a sour, rancid and soapy taste indicated the presence of concentrations at microg/ml levels of ethyl butanoate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, octanoic acid, decanoic acid and dodecanoic acid. the off-odour and taste were attributed to the presence of these compounds in the milk. microbiological examination confirmed that the milk was also contaminated with a seri ... | 2000 | 11131069 |
prevalence and growth of pathogens on salad vegetables, fruits and sprouts. | a total of 120 samples, comprising different types of raw vegetables (seven), fruits (three) and sprouts (three) obtained from street vendors, were tested for aerobic plate count, coliform count and various food-borne pathogens. average aerobic plate counts for salad vegetables, fruits and sprouts were greater than 10(10) cfu/g and 10(9) cfu/g respectively. pathogens isolated were s. aureus, e. coli, enterobacter sp., klebsiella sp., s. typhi, serratia sp., providencia sp. and p. aeruginosa. the ... | 2001 | 11279816 |
diuretic effects of selected thai indigenous medicinal plants in rats. | extracts of five indigenous thai medicinal having ethnomedical application in the treatment of dysuria were investigated for their diuretic activity. root extracts of ananas comosus and carica papaya, given orally to rats at a dose of 10 mg/kg, demonstrated significantly increased urine output (p < 0.01) which was 79 and 74%, respectively, of the effect of an equivalent dose of hydrochlorothiazide. both plant extracts gave similar profiles of urinary electrolyte excretion to that of the hydrochl ... | 2001 | 11297849 |
16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril). | nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria. | 2001 | 11319127 |
a study on transfer factors of 60co and 65zn from soil to plants in the tropical environment of bangladesh. | soil to plant transfer factor (tf) of 60co and 65zn was determined from radioisotope experiments on plants grown in pots under outdoor ambient tropical conditions for three growing seasons (1995-1998). the tfs were obtained for different plants/crops such as, rice, bean, peanuts pineapple, cabbage, tomato, spinach and grass. the average tf values of 60co are found to be 0.087. 0.15, 0.12, 0.67, 0.28, 0.79, 1.03 and 0.34 respectively for the above mentioned plants/crops. in case of 65zn, the aver ... | 2001 | 11336414 |
ingestion of insoluble dietary fibre increased zinc and iron absorption and restored growth rate and zinc absorption suppressed by dietary phytate in rats. | we examined the effects of ingestion of five types of insoluble fibre on growth and zn absorption in rats fed a marginally zn-deficient diet (6.75 mg (0.103 mmol) zn/kg diet) with or without added sodium phytate (12.6 mmol/kg diet). the types of insoluble fibre tested were corn husks, watermelon skin, yam-bean root (pachyrhizus erosus) and pineapple core, and cellulose was used as a control (100 g/kg diet). body-weight gain in the cellulose groups was suppressed by 57 % by feeding phytate. body- ... | 2001 | 11591231 |
comparison of culture media, simplate, and petrifilm for enumeration of yeasts and molds in food. | the efficacy of three culture media, dichloran rose bengal chloramphenicol (drbc), dichloran 18% glycerol agar (dg18), and potato dextrose agar (pda) supplemented with two antibiotics, were compared with the simplate and petrifilm techniques for mold and yeast enumeration. the following foods were analyzed: corn meal, wheat flour, cassava flour, bread crumbs, whole meal, sliced bread, ground peanuts, mozzarella cheese, grated parmesan cheese, cheese rolls, orange juice, pineapple pulp, pineapple ... | 2001 | 11601710 |
characterization of yeast strains for wine production: effect of fermentation variables on quality of wine produced. | sixteen yeast strains isolated from grapefruit (citrus paradis), orange (citrus sinensis) and pineapple (ananas comosus) were characterized using standard microbiological procedures. the species were identified as saccharomyces uvarum, s. cerevisiae, s. carlbergensis, and s. ellipsoideus. their abilities for wine production were tested by using sugar and ethanol tolerance tests. the best biochemically active strain, s. ellipsoideus, was used along with commercially available baker's yeast (s. ce ... | 2001 | 11732717 |
antibody binding to venom carbohydrates is a frequent cause for double positivity to honeybee and yellow jacket venom in patients with stinging-insect allergy. | up to 50% of patients with stinging-insect allergy have double-positive rast results to honeybee and yellow jacket (yj) venom. true double sensitization and crossreactivity through venom hyaluronidases are considered main reasons for this multiple reactivity. | 2001 | 11742287 |
proteolytic inhibition of salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases erk and jnk in cultured human intestinal cells. | bromelain, a mixture of cysteine proteases from pineapple stems, blocks signaling by the mitogen-activated protein (map) kinases extracellular regulated kinase 1 (erk-1) and erk-2, inhibits inflammation, and protects against enterotoxigenic escherichia coli infection. in this study, we examined the effect of bromelain on salmonella enterica serovar typhimurium infection, since an important feature of its pathogenesis is its ability to induce activation of erk-1 and erk-2, which leads to internal ... | 2002 | 11748167 |
small bowel review: normal physiology part 1. | in the past year there have been many advances in the area of small bowel physiology and pathology and therapy. in preparation for this review, over 1500 papers were assessed. the focus is on presenting clinically useful information for the practising gastroenterologist. selected important clinical learning points include the following: (1) glucose absorption mediated by sglt1 is controlled by mrna abundance, as well as by posttranscriptional processes including protein trafficking; (2) inducers ... | 2001 | 11768247 |
evaluation of the antioxidant properties of mediterranean and tropical fruits compared with common food additives. | several mediterranean and tropical fruits have been analyzed in order to assess their antioxidant activity compared with that of common food additives (butylated hydroxyanisole [bha], butylated hydroxytoluene [bht] and propyl gallate). among mediterranean fruits, red grape and plum were more effective (p < 0.05) scavengers of peroxyl radicals than bha, bht, and propyl gallate. of the tropical fruits, banana was the most effective scavenger of peroxyl radicals. mediterranean and tropical fruits s ... | 2001 | 11770635 |
bromelain reversibly inhibits invasive properties of glioma cells. | bromelain is an aqueous extract from pineapple stem that contains proteinases and exhibits pleiotropic therapeutic effects, i.e., antiedematous, antiinflammatory, antimetastatic, antithrombotic, and fibrinolytic activities. in this study, we tested bromelain's effects on glioma cells to assess whether bromelain could be a potential contributor to new antiinvasive strategies for gliomas. several complementary assays demonstrated that bromelain significantly and reversibly reduced glioma cell adhe ... | 2001 | 11774029 |
ige reactivity to profilin in pollen-sensitized subjects with adverse reactions to banana and pineapple. | the so-called 'latex-fruit syndrome' is a well-documented phenomenon in cross-reactive allergies. by contrast, there is a lack of information about allergy to exotic fruits in patients with a predominant pollen sensitization. since the ubiquitous protein profilin has been identified as an allergen in natural rubber latex as well as in pollen-related foods, the aim of this study was to investigate the role of profilin in allergy to certain exotic fruits. | 2002 | 12065910 |
new sources of dietary myosmine uptake from cereals, fruits, vegetables, and milk. | myosmine has been regarded as a specific tobacco alkaloid until investigations pointed out that nuts and nut products constitute a significant source of myosmine. in the present study it is shown that the occurrence of myosmine is widespread throughout a large number of plant families. using a method for extraction practicable for all examined foods, quantitative analysis through internal standard addition showed nanograms per gram amounts. positively tested edibles were staple foods such as mai ... | 2002 | 12166981 |
swaziland. | swaziland is a country of 17,363 sq km with 860,000 inhabitants, of whom 64% are literate. independence was gained on september 6, 1968. the terrain consists of mountains and plateaus, with a climate variously near-temperature, subtropical, and semi-arid. english and siswati are spoken by swazi, zulu, and non-african ethnic groups, who variously hold christian and indigenous beliefs. life expectancy ranges between 53 and 60 years, gdp is $704 million, growing at a rate of 7%. per capita in ... | 1993 | 12178057 |
antioxidant and antiproliferative activities of common fruits. | consumption of fruits and vegetables has been associated with reduced risk of chronic diseases such as cardiovascular disease and cancer. phytochemicals, especially phenolics, in fruits and vegetables are suggested to be the major bioactive compounds for the health benefits. however, the phenolic contents and their antioxidant activities in fruits and vegetables were underestimated in the literature, because bound phenolics were not included. this study was designed to investigate the profiles o ... | 2002 | 12452674 |
orbital reconstruction after exenteration: use of a transorbital temporal muscle flap. | orbital exenteration is a disfiguring operation that involves the total removal of the orbital contents with partial or total excision of the eyelids. common methods of orbit reconstruction include pectoralis musculocutaneous pedicled flap and free tissue transfer. the purpose of this study is to illustrate that the entire temporalis muscle may be used by creating a large window in the lateral orbit, without resection of the lateral orbital rim. orbital exenteration was performed on four cadaver ... | 2003 | 12545107 |