Publications

TitleAbstractYear
Filter
PMID
Filter
characterization of a plant sine, p-sine1, in rice genomes.we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ...19921326298
factors influencing agrobacterium-mediated transient expression of gusa in rice.transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ...19921463839
photosynthetic bradyrhizobia are natural endophytes of the african wild rice oryza breviligulata.we investigated the presence of endophytic rhizobia within the roots of the wetland wild rice oryza breviligulata, which is the ancestor of the african cultivated rice oryza glaberrima. this primitive rice species grows in the same wetland sites as aeschynomene sensitiva, an aquatic stem-nodulated legume associated with photosynthetic strains of bradyrhizobium. twenty endophytic and aquatic isolates were obtained at three different sites in west africa (senegal and guinea) from nodal roots of o. ...200011097925
genetic variations of aa genome oryza species measured by mite-aflp.mites (miniature inverted-repeat transposable elements) are the major transposable elements in oryza species. we have applied the mite-aflp technique to study the genetic variation and species relationship in the aa-genome oryza species. high polymorphism was detected within and between species. the genetic variation in the cultivated species, oryza sativa and oryza glaberrima, was comparatively lower than in their ancestral wild species. in comparison between geographical lineages of the aa gen ...200312845436
a comparative study of genetic relationships among the aa-genome oryza species using rapd and ssr markers.in order to estimate genetic relationships of the aa-genome oryza species, rapd and ssr analyses were performed with 45 accessions, including 13 cultivated varieties (eight oryza sativa and five oryza glaberrima) and 32 wild accessions (nine oryza rufipogon, seven oryza nivara, three oryza glumaepatula, four oryza longistaminata, six oryza barthii, and three oryza meridionalis). a total of 181 clear and repeatable bands were amplified from 27 selected rapd primers, and 101 alleles were detected ...200314504744
qtl mapping of grain quality traits from the interspecific cross oryza sativa x o. glaberrima.international rice export markets are increasing demands for rapid improvements in grain quality characteristics. the african rice oryza glaberrima is a new potential source of genes that will enhance the eating, cooking, and milling properties of the rice grain. the objective of this research was to identify and characterize quantitative trait loci (qtls) among 312 doubled haploid lines derived from the bc3f1 of an interspecific cross of o. sativa x o. glaberrima. genetic material was planted i ...200415105992
the oryza map alignment project: the golden path to unlocking the genetic potential of wild rice species.the wild species of the genus oryza offer enormous potential to make a significant impact on agricultural productivity of the cultivated rice species oryza sativa and oryza glaberrima. to unlock the genetic potential of wild rice we have initiated a project entitled the 'oryza map alignment project' (omap) with the ultimate goal of constructing and aligning bac/stc based physical maps of 11 wild and one cultivated rice species to the international rice genome sequencing project's finished refere ...200516217601
evaluation of four sampling techniques for surveillance of culex quinquefasciatus (diptera: culicidae) and other mosquitoes in african rice agroecosystems.field studies were conducted in a rice, oryza sativa l., agroecosystem in mwea kenya to compare the efficiency of co2-baited centers for disease control (cdc) light traps against nonbaited cdc light traps and gravid traps against oviposition traps in outdoor collection of culex quinquefasciatus say (diptera: culicidae) and other mosquitoes. collectively, 21 mosquito species from the genera culex, anopheles, mansonia, ficalbia, and aedes were captured during the 10-wk study period. cx. quinquefas ...200717547238
identification of a rice stripe necrosis virus resistance locus and yield component qtls using oryza sativa x o. glaberrima introgression lines.developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for qtl detection and breeding programs.201020064202
massive gene losses in asian cultivated rice unveiled by comparative genome analysis.rice is one of the most important food crops in the world. with increasing world demand for food crops, there is an urgent need to develop new cultivars that have enhanced performance with regard to yield, disease resistance, and so on. wild rice is expected to provide useful genetic resources that could improve the present cultivated species. however, the quantity and quality of these unexplored resources remain unclear. recent accumulation of the genomic information of both cultivated and wild ...201020167122
identification of a second major resistance gene to rice yellow mottle virus, rymv2, in the african cultivated rice species, o. glaberrima.rice yellow mottle virus (rymv) is the most damaging rice-infecting virus in africa. however, few sources of high resistance and only a single major resistance gene, rymv1, are known to date. we screened a large representative collection of african cultivated rice (oryza glaberrima) for rymv resistance. whereas high resistance is known to be very rare in asian cultivated rice (oryza sativa), we identified 29 (8%) highly resistant accessions in o. glaberrima. the mif4g domain of rymv1 was sequenc ...201020198467
rice structural variation: a comparative analysis of structural variation between rice and three of its closest relatives in the genus oryza.rapid progress in comparative genomics among the grasses has revealed similar gene content and order despite exceptional differences in chromosome size and number. large- and small-scale genomic variations are of particular interest, especially among cultivated and wild species, as they encode rapidly evolving features that may be important in adaptation to particular environments. we present a genome-wide study of intermediate-sized structural variation (sv) among rice (oryza sativa) and three ...201020626650
the adaptation of rice yellow mottle virus to the eif(iso)4g-mediated rice resistance.the rymv1-3 allele of the eif(iso)4g-mediated resistance to rice yellow mottle virus (rymv) is found in a few oryza glaberrima cultivars. the same resistance-breaking (rb) mutations emerged in the central domain of the vpg after inoculation of isolates of different strains. the rb mutations were fixed, often sequentially, at codons 41 and 52 which paralleled an increase in virus accumulation. rb mutations also emerged after inoculation of an avirulent infectious clone, indicating that they were ...201020888025
dynamic nucleotide-binding site and leucine-rich repeat-encoding genes in the grass family.the proper use of resistance genes (r genes) requires a comprehensive understanding of their genomics and evolution. we analyzed genes encoding nucleotide-binding sites and leucine-rich repeats in the genomes of rice (oryza sativa), maize (zea mays), sorghum (sorghum bicolor), and brachypodium distachyon. frequent deletions and translocations of r genes generated prevalent presence/absence polymorphism between different accessions/species. the deletions were caused by unequal crossover, homologo ...201222422941
linkage mapping of hsa-1(og), a resistance gene of african rice to the cyst nematode, heterodera sacchari.inheritance of resistance to cyst nematode (heterodera sacchari) in oryza sativa was investigated by inoculation tests with isolate 244 from congo in segregating populations derived from hybridisation between o. sativa and its african sister cultivated species, o. glaberrima. we found that the resistance was controlled by one major gene, hsa-1(og), with codominance of susceptible and resistant alleles. to map hsa-1(og) on the rice genome, we pooled the data obtained from segregation of the resis ...200312721640
transcriptomic and histological responses of african rice (oryza glaberrima) to meloidogyne graminicola provide new insights into root-knot nematode resistance in monocots.the root-knot nematode meloidogyne graminicola is responsible for production losses in rice ( oryza sativa ) in asia and latin america. the accession tog5681 of african rice, o. glaberrima , presents improved resistance to several biotic and abiotic factors, including nematodes. the aim of this study was to assess the cytological and molecular mechanisms underlying nematode resistance in this accession.201728334204
meloidogyne graminicola: a major threat to rice agriculture.superkingdom eukaryota; kingdom metazoa; phylum nematoda; class chromadorea; order tylenchida; suborder tylenchina; infraorder tylenchomorpha; superfamily tylenchoidea; family meloidogynidae; subfamily meloidogyninae; genus meloidogyne.201726950515
plant-parasitic nematode infections in rice: molecular and cellular insights.being one of the major staple foods in the world, and an interesting model monocot plant, rice (oryza sativa l.) has recently received attention from molecular nematologists studying the cellular and molecular aspects of the interaction between this crop and plant-parasitic nematodes. in this review, we highlight recent advances in this field, with a focus on the best-studied root-knot nematodes. histological studies have revealed the cellular changes inside root-knot nematode-induced feeding si ...201424906129
suppression of cell wall-related genes associated with stunting of oryza glaberrima infected with rice tungro spherical virus.rice tungro disease is a complex disease caused by the interaction between rice tungro bacilliform virus and rice tungro spherical virus (rtsv). rtsv alone does not cause recognizable symptoms in most asian rice (oryza sativa) plants, whereas some african rice (o. glaberrima) plants were found to become stunted by rtsv. stunting of rice plants by virus infections usually accompanies the suppression of various cell wall-related genes. the expression of cell wall-related genes was examined in o. g ...201424550897
fine mapping of rymv3: a new resistance gene to rice yellow mottle virus from oryza glaberrima.a new resistance gene against rice yellow mottle virus was identified and mapped in a 15-kb interval. the best candidate is a cc-nbs-lrr gene. rice yellow mottle virus (rymv) disease is a serious constraint to the cultivation of rice in africa and selection for resistance is considered to be the most effective management strategy. the aim of this study was to characterize the resistance of tog5307, a highly resistant accession belonging to the african cultivated rice species (oryza glaberrima), ...201728144699
a recessive resistance to rice yellow mottle virus is associated with a rice homolog of the cpr5 gene, a regulator of active defense mechanisms.rymv2 is a major recessive resistance gene identified in cultivated african rice (oryza glaberrima) which confers high resistance to the rice yellow mottle virus (rymv). we mapped rymv2 in an approximately 30-kb interval in which four genes have been annotated. sequencing of the candidate region in the resistant tog7291 accession revealed a single mutation affecting a predicted gene, as compared with the rymv-susceptible o. glaberrima cg14 reference sequence. this mutation was found to be a one- ...201323944999
resilience of rice (oryza spp.) pollen germination and tube growth to temperature stress.resilience of rice cropping systems to potential global climate change will partly depend on the temperature tolerance of pollen germination (pg) and tube growth (ptg). pollen germination of high temperature-susceptible oryza glaberrima steud. (cv. cg14) and oryza sativa l. ssp. indica (cv. ir64) and high temperature-tolerant o. sativa ssp. aus (cv. n22), was assessed on a 5.6-45.4 °c temperature gradient system. mean maximum pg was 85% at 27 °c with 1488 μm ptg at 25 °c. the hypothesis that in ...201625346255
sustainable reuse of rice residues as feedstocks in vermicomposting for organic fertilizer production.over the past decade, rice (oryza sativa or oryza glaberrima) cultivation has increased in many rice-growing countries due to the increasing export demand and population growth and led to a copious amount of rice residues, consisting mainly of rice straw (rs) and rice husk (rh), being generated during and after harvesting. in this study, eudrilus eugeniae was used to decompose rice residues alone and rice residues amended with cow dung (cd) for bio-transformation of wastes into organic fertilize ...201423900949
pre-attachment striga hermonthica resistance of new rice for africa (nerica) cultivars based on low strigolactone production.striga hermonthica (striga) is an obligate hemiparasitic weed, causing severe yield losses in cereals, including rice, throughout sub-saharan africa. striga germination depends on strigolactones (germination stimulants) exuded by the host roots. the interspecific new rice for africa (nerica) cultivars offer a potentially interesting gene pool for a screen for low germination-inducing rice cultivars. exudates were collected from all nerica cultivars and their parents (oryza sativa and oryza glabe ...021883233
long-range and targeted ectopic recombination between the two homeologous chromosomes 11 and 12 in oryza species.whole genome duplication (wgd) and subsequent evolution of gene pairs have been shown to have shaped the present day genomes of most, if not all, plants and to have played an essential role in the evolution of many eukaryotic genomes. analysis of the rice (oryza sativa ssp. japonica) genome sequence suggested an ancestral wgd ∼50-70 ma common to all cereals and a segmental duplication between chromosomes 11 and 12 as recently as 5 ma. more recent studies based on coding sequences have demonstrat ...021616911
evidence for mid-holocene rice domestication in the americas.the development of agriculture is one of humankind's most pivotal achievements. questions about plant domestication and the origins of agriculture have engaged scholars for well over a century, with implications for understanding its legacy on global subsistence strategies, plant distribution, population health and the global methane budget. rice is one of the most important crops to be domesticated globally, with both asia (oryza sativa l.) and africa (oryza glaberrima steud.) discussed as prim ...201728993622
a single-nucleotide polymorphism causes smaller grain size and loss of seed shattering during african rice domestication.grain size is one of the most important components of grain yield and selecting large seeds has been a main target during plant domestication. surprisingly, the grain of african cultivated rice (oryza glaberrima steud.) typically is smaller than that of its progenitor, oryza barthii. here we report the cloning and characterization of a quantitative trait locus, gl4, controlling the grain length on chromosome 4 in african rice, which regulates longitudinal cell elongation of the outer and inner g ...201728481332
differences in meristem size and expression of branching genes are associated with variation in panicle phenotype in wild and domesticated african rice.the african rice oryza glaberrima was domesticated from its wild relative oryza barthii about 3000 years ago. during the domestication process, panicle complexity changed from a panicle with low complexity in o. barthii, to a highly branched panicle carrying more seeds in o. glaberrima. to understand the basis of this differential panicle development between the two species, we conducted morphological and molecular analyses of early panicle development.201728149498
a single base change explains the independent origin of and selection for the nonshattering gene in african rice domestication.reduced seed shattering was a critical evolutionary step in crop domestication. two cultivated rice species, oryza sativa and oryza glaberrima, were independently domesticated from the wild species oryza rufipogon in asia and oryza barthii in africa, respectively. a single nucleotide polymorphism (snp) in the c gene, which encodes a trihelix transcription factor, causes nonshattering in o. sativa. however, the genetic mechanism of nonshattering in o. glaberrima is poorly understood. we conducted ...201727861933
mir2118-triggered phased sirnas are differentially expressed during the panicle development of wild and domesticated african rice species.rice exhibits a wide range of panicle structures. to explain these variations, much emphasis has been placed on changes in transcriptional regulation, but no large-scale study has yet reported on changes in small rna regulation in the various rice species. to evaluate this aspect, we performed deep sequencing and expression profiling of small rnas from two closely related species with contrasting panicle development: the cultivated african rice oryza glaberrima and its wild relative oryza barthi ...201626969003
the biosynthesis, structure and gelatinization properties of starches from wild and cultivated african rice species (oryza barthii and oryza glaberrima).the molecular structure and gelatinization properties of starches from domesticated african rice (oryza glaberrima) and its wild progenitor (oryza barthii) are determined and comparison made with asian domesticated rice (oryza sativa), the commonest commercial rice. this suggests possible enzymatic processes contributing to the unique traits of the african varieties. these have similar starch structures, including smaller amylose molecules, but larger amounts of amylose chains across the whole a ...201526050893
rapid diversification of five oryza aa genomes associated with rice adaptation.comparative genomic analyses among closely related species can greatly enhance our understanding of plant gene and genome evolution. we report de novo-assembled aa-genome sequences for oryza nivara, oryza glaberrima, oryza barthii, oryza glumaepatula, and oryza meridionalis. our analyses reveal massive levels of genomic structural variation, including segmental duplication and rapid gene family turnover, with particularly high instability in defense-related genes. we show, on a genomic scale, ho ...201425368197
the genome sequence of african rice (oryza glaberrima) and evidence for independent domestication.the cultivation of rice in africa dates back more than 3,000 years. interestingly, african rice is not of the same origin as asian rice (oryza sativa l.) but rather is an entirely different species (i.e., oryza glaberrima steud.). here we present a high-quality assembly and annotation of the o. glaberrima genome and detailed analyses of its evolutionary history of domestication and selection. population genomics analyses of 20 o. glaberrima and 94 oryza barthii accessions support the hypothesis ...201425064006
discovery of microrna-target modules of african rice (oryza glaberrima) under salinity stress.oryza glaberrima is the second edible rice in the genus oryza. it is grown in the african countries. mirnas are regulatory molecules that are involved in every domains of gene expression including salinity stress response. although several mirnas have been reported from various species of oryza, yet none of them are from this species. salt treated (200 mm nacl for 48 h) and control smallrna libraries of ram-100, a salt tolerant genotype, each with 2 replications generated 150 conserve and 348 no ...201829330361
a hypersensitivity-like response to meloidogyne graminicola in rice (oryza sativa l.).meloidogyne graminicola (m. graminicola) is a major plant-parasitic nematode affecting rice cultivation in asia. resistance to this nematode was found in the african rice genotypes oryza glaberrima and o. longistaminata, however, due to interspecific hybrid sterility, the introgression of resistance genes in the widely consumed o. sativa varieties remains challenging. recently, resistance was found in o. sativa and here we report for the first time the histological and genetic characterization o ...201729161206
genetic variation and population structure of oryza glaberrima and development of a mini-core collection using dartseq.the sequence variation present in accessions conserved in genebanks can best be used in plant improvement when it is properly characterized and published. using low cost and high density single nucleotide polymorphism (snp) assays, the genetic diversity, population structure, and relatedness between pairs of accessions can be quickly assessed. this information is relevant for different purposes, including creating core and mini-core sets that represent the maximum possible genetic variation cont ...201729093721
macromolecular traits in the african rice oryza glaberrima and in glaberrima/sativa crosses, and their relevance to processing.molecular properties of proteins and starch were investigated in 2 accessions of oryza glaberrima and oryza sativa, and in one nerica cross between the 2 species, to assess traits that could be relevant to transformation into specific foods. protein nature and organization in o. glaberrima were different from those in o. sativa and in nerica. despite the similar cysteine content in all samples, thiol accessibility in o. glaberrima proteins was higher than in nerica or in o. sativa. inter-protein ...201728850662
sequencing of bulks of segregants allows dissection of genetic control of amylose content in rice.amylose content (ac) is a key quality trait in rice. a cross between oryza glaberrima (african rice) and oryza sativa (asian rice) segregating for ac was analysed by sequencing bulks of individuals with high and low ac. snp associated with the granule bound starch synthase (gbss1) locus on chromosome 6 were polymorphic between the bulks. in particular, a g/a snp that would result in an asp to asn mutation was identified. this amino acid substitution may be responsible for differences in gbss act ...201828499072
a walk on the wild side: oryza species as source for rice abiotic stress tolerance.oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population increase are expected to soon call for new, fast generation of stress tolerant rice germplasm, and current within-species rice diversity might not be enough to overcome such needs. the ...201728323300
soybean-enriched snacks based on african rice.snacks were produced by extruding blends of partially-defatted soybean flour with flours from milled or parboiled african-grown rice. the interplay between composition and processing in producing snacks with a satisfactory sensory profile was addressed by e-sensing, and by molecular and rheological approaches. soybean proteins play a main role in defining the properties of the protein network in the products. at the same content in soybean flour, use of parboiled rice flour increases the snack's ...201628231133
genetic variation and host-parasite specificity of striga resistance and tolerance in rice: the need for predictive breeding.the parasitic weeds striga asiatica and striga hermonthica cause devastating yield losses to upland rice in africa. little is known about genetic variation in host resistance and tolerance across rice genotypes, in relation to virulence differences across striga species and ecotypes. diverse rice genotypes were phenotyped for the above traits in s. asiatica- (tanzania) and s. hermonthica-infested fields (kenya and uganda) and under controlled conditions. new rice genotypes with either ecotype-sp ...201728191641
de novo assemblies of three oryza glaberrima accessions provide first insights about pan-genome of african rices.oryza glaberrima is one of the two cultivated species of rice, and harbors various interesting agronomic traits, especially in biotic and abiotic resistance, compared with its asian cousin o. sativa. a previous reference genome was published but newer studies highlighted some missing parts. moreover, global species diversity is known nowadays to be represented by more than one single individual. for that purpose, we sequenced, assembled and annotated de novo three different cultivars from o. gla ...201728173009
electrophysiological and behavioral responses of female african rice gall midge, orseolia oryzivora harris and gagné, to host plant volatiles.african rice gall midge, orseolia oryzivora harris and gagné, is a major pest of rice in africa. depsite its economic importance, its chemical ecology is not well understood. here, we assessed behavioral and electrophysiological responses of o. oryzivora to host plant volatiles. in olfactometer bioassays, mated female o. oryzivora were attracted to volatiles emitted from intact rice plants but were repelled by volatiles collected from plants infested by conspecifics. in a choice test, there was ...201727815665
rice chloroplast genome variation architecture and phylogenetic dissection in diverse oryza species assessed by whole-genome resequencing.chloroplast genome variations have been detected, despite its overall conserved structure, which has been valuable for plant population genetics and evolutionary studies. here, we described chloroplast variation architecture of 383 rice accessions from diverse regions and different ecotypes, in order to mine the rice chloroplast genome variation architecture and phylogenetic.201627757948
tracing ancestor rice of suriname maroons back to its african origin.african rice (oryza glaberrima) and african cultivation practices are said to have influenced emerging colonial plantation economies in the americas1,2. however, the level of impact of african rice practices is difficult to establish because of limited written or botanical records2,3. recent findings of o. glaberrima in rice fields of suriname maroons bear evidence of the high level of knowledge about rice among african slaves and their descendants, who consecrate it in ancestor rituals4,5. here ...201627694825
genetic diversity and phenotypic variation in an introgression line population derived from an interspecific cross between oryza glaberrima and oryza sativa.the introduction of closely related species genomic fragments is an effective way to enrich genetic diversity and creates new germplasms in crops. here, we studied the genetic diversity of an introgression line (il) population composed of 106 ils derived from an interspecific tetra cross between o. glaberrima and o. sativa (ram3/jin23b//jin23b///yuetaib). the proportion of o. glaberrima genome (pgg) in the ils ranged from 0.3% to 36.7%, with an average value of 12.32% which is close to the theor ...201627603678
dna transposon activity is associated with increased mutation rates in genes of rice and other grasses.dna (class 2) transposons are mobile genetic elements which move within their 'host' genome through excising and re-inserting elsewhere. although the rice genome contains tens of thousands of such elements, their actual role in evolution is still unclear. analysing over 650 transposon polymorphisms in the rice species oryza sativa and oryza glaberrima, we find that dna repair following transposon excisions is associated with an increased number of mutations in the sequences neighbouring the tran ...201627599761
domestication history and geographical adaptation inferred from a snp map of african rice.african rice (oryza glaberrima steud.) is a cereal crop species closely related to asian rice (oryza sativa l.) but was independently domesticated in west africa ∼3,000 years ago. african rice is rarely grown outside sub-saharan africa but is of global interest because of its tolerance to abiotic stresses. here we describe a map of 2.32 million snps of african rice from whole-genome resequencing of 93 landraces. population genomic analysis shows a population bottleneck in this species that began ...201627500524
loss of function at rae2, a previously unidentified epfl, is required for awnlessness in cultivated asian rice.domestication of crops based on artificial selection has contributed numerous beneficial traits for agriculture. wild characteristics such as red pericarp and seed shattering were lost in both asian (oryza sativa) and african (oryza glaberrima) cultivated rice species as a result of human selection on common genes. awnedness, in contrast, is a trait that has been lost in both cultivated species due to selection on different sets of genes. in a previous report, we revealed that at least three loc ...201627466405
structure of clusters and building blocks in amylopectin from african rice accessions.enzymatic hydrolysis in combination with gel-permeation and anion-exchange chromatography techniques were employed to characterise the composition of clusters and building blocks of amylopectin from two african rice (oryza glaberrima) accessions-irgc 103759 and tog 12440. the samples were compared with one asian rice (oryza sativa) sample (cv wita 4) and one o. sativa×o. glaberrima cross (nerica 4). the average dp of clusters from the african rice accessions (aras) was marginally larger (dp=83) ...201627185123
the impact and origin of copy number variations in the oryza species.copy number variation (cnv), a complex genomic rearrangement, has been extensively studied in humans and other organisms. in plants, cnvs of several genes were found to be responsible for various important traits; however, the cause and consequence of cnvs remains largely unknown. recently released next-generation sequencing (ngs) data provide an opportunity for a genome-wide study of cnvs in rice.201627025496
unit and internal chain profile of african rice (oryza glaberrima) amylopectin.high-performance anion-exchange chromatography was used to study the unit chain profiles of amylopectins and their φ,β-limit dextrins from two african rice (oryza glaberrima) accessions-tog 12440 and irgc 103759. the samples were compared with two asian rice (oryza sativa) samples (cv koshihikari and cv wita 4) and one o. sativa × o. glaberrima cross (nerica 4). the ratio of short:long chains ranged between 12.1 and 13.8, and the ratio of a:b-chains was ∼ 1.0 in all samples. a significant differ ...201626686152
genome-wide survey and expression analysis of the osca gene family in rice.reception of and response to exogenous and endogenous osmotic changes is important to sustain plant growth and development, as well as reproductive formation. hyperosmolality-gated calcium-permeable channels (osca) were first characterised as an osmosensor in arabidopsis and are involved in the perception of extracellular changes to trigger hyperosmolality-induced [ca(2+)]i increases (oici). to explore the potential biological functions of oscas in rice, we performed a bioinformatics and express ...201526503287
convergent loss of awn in two cultivated rice species oryza sativa and oryza glaberrima is caused by mutations in different loci.a long awn is one of the distinct morphological features of wild rice species. this organ is thought to aid in seed dispersal and prevent predation by animals. most cultivated varieties of oryza sativa and oryza glaberrima, however, have lost the ability to form long awns. the causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. here, we evaluated three sets of chromosome segment substitution lines (cssls) in a common o. sativa genetic backgrou ...201526338659
fine mapping of s37, a locus responsible for pollen and embryo sac sterility in hybrids between oryza sativa l. and o. glaberrima steud.hybrid sterility locus s37 between oryza glaberrima and oryza sativa results in both pollen and embryo sac sterility. interspecific crossing between african cultivated rice oryza glaberrima and oryza sativa cultivars is hindered by hybrid sterility. to dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (nil)-s37 using dianjingyou1 (djy1) as the recipient parent and an african cultivated rice variety as the donor parent. empty pollen and embryo sac sterilit ...201526169392
do nerica rice cultivars express resistance to striga hermonthica (del.) benth. and striga asiatica (l.) kuntze under field conditions?the parasitic weeds striga asiatica and striga hermonthica cause high yield losses in rain-fed upland rice in africa. two resistance classes (pre- and post-attachment) and several resistant genotypes have been identified among nerica (new rice for africa) cultivars under laboratory conditions (in vitro) previously. however, little is known about expression of this resistance under field conditions. here we investigated (1) whether resistance exhibited under controlled conditions would express un ...201526089591
natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of african rice.global warming threatens many aspects of human life, for example, by reducing crop yields. breeding heat-tolerant crops using genes conferring thermotolerance is a fundamental way to help deal with this challenge. here we identify a major quantitative trait locus (qtl) for thermotolerance in african rice (oryza glaberrima), thermo-tolerance 1 (tt1), which encodes an α2 subunit of the 26s proteasome involved in the degradation of ubiquitinated proteins. ubiquitylome analysis indicated that ogtt1 ...201525985140
genome-wide comparison of asian and african rice reveals high recent activity of dna transposons.dna (class ii) transposons are ubiquitous in plant genomes. however, unlike for (class i) retrotransposons, only little is known about their proliferation mechanisms, activity, and impact on genomes. asian and african rice (oryza sativa and o. glaberrima) diverged approximately 600,000 years ago. their fully sequenced genomes therefore provide an excellent opportunity to study polymorphisms introduced from recent transposon activity.201525954322
rapid turnover of antimicrobial-type cysteine-rich protein genes in closely related oryza genomes.defensive and reproductive protein genes undergo rapid evolution. small, cysteine-rich secreted peptides (crps) act as antimicrobial agents and function in plant intercellular signaling and are over-represented among reproductively expressed proteins. because of their roles in defense, reproduction and development and their presence in multigene families, crp variation can have major consequences for plant phenotypic and functional diversification. we surveyed the crp genes of six closely relate ...201525842177
comparative population genomics reveals strong divergence and infrequent introgression between asian and african rice. 201525615591
an evolutionary genomic tale of two rice species.the domestication of african rice, oryza glaberrima, occurred separately from that of the much more widespread asian rice species oryza sativa. analysis of the whole-genome sequence for o. glaberrima shows the extent to which the same genes were involved in these distinct but parallel evolutionary events.201425162803
an extensive analysis of the african rice genetic diversity through a global genotyping.we present here the first curated collection of wild and cultivated african rice species. for that, we designed specific snps and were able to structure these very low diverse species. oryza glaberrima, the cultivated african rice, is endemic from africa. this species and its direct ancestor, o. barthii, are valuable tool for improvement of asian rice o. sativa in terms of abiotic and biotic stress resistance. however, only a few limited studies about the genetic diversity of these species were ...201425119871
mapping three new interspecific hybrid sterile loci between oryza sativa and o. glaberrima.hybrid sterility hinders the transfer of useful traits between oryza sativa and o. glaberrima. in order to further understand the nature of interspecific hybrid sterility between these two species, a strategy of multi-donors was used to elucidate the range of interspecific hybrid sterility in this study. fifty-nine accessions of o. glaberrima were used as female parents for hybridization with japonica cultivar dianjingyou 1, after several backcrossings using dianjingyou 1 as the recurrent parent ...201424757387
a novel allele of the p-starvation tolerance gene ospstol1 from african rice (oryza glaberrima steud) and its distribution in the genus oryza.we have developed allele-specific markers for molecular breeding to transfer the pstol1 gene from kasalath to african mega-varieties, including nericas, to improve their tolerance to p-deficient soil. the deficiency of phosphorus (p) in soil is a major problem in sub-saharan africa due to general nutrient depletion and the presence of p-fixing soils. developing rice cultivars with enhanced p efficiency would, therefore, represent a sustainable strategy to improve the livelihood of resource-poor ...201424728072
could abiotic stress tolerance in wild relatives of rice be used to improve oryza sativa?oryza sativa and oryza glaberrima have been selected to acquire and partition resources efficiently as part of the process of domestication. however, genetic diversity in cultivated rice is limited compared to wild oryza species, in spite of 120,000 genotypes being held in gene banks. by contrast, there is untapped diversity in the more than 20 wild species of oryza, some having been collected from just a few coastal locations (e.g. oryza schlechteri), while others are widely distributed (e.g. o ...201424388514
aroma chemistry of african oryza glaberrima and oryza sativa rice and their interspecific hybrids.to increase rice production in africa, considerable research has focused on creating interspecific hybrids between african (oryza glaberrima steud.) and asian (o. sativa l.) rice in an attempt to obtain the positive attributes of each in new cultivars. since flavor is a key criterion in consumer acceptance of rice, as an initial inquiry we characterized and compared the aroma chemistry of selected cultivars of african o. sativa ssp. japonica, o. sativa ssp. indica, o. glaberrima, and their inter ...201423907855
the role of bh4 in parallel evolution of hull colour in domesticated and weedy rice.the two independent domestication events in the genus oryza that led to african and asian rice offer an extremely useful system for studying the genetic basis of parallel evolution. this system is also characterized by parallel de-domestication events, with two genetically distinct weedy rice biotypes in the us derived from the asian domesticate. one important trait that has been altered by rice domestication and de-domestication is hull colour. the wild progenitors of the two cultivated rice sp ...201323859433
coordination of leaf photosynthesis, transpiration, and structural traits in rice and wild relatives (genus oryza).the genus oryza, which includes rice (oryza sativa and oryza glaberrima) and wild relatives, is a useful genus to study leaf properties in order to identify structural features that control co(2) access to chloroplasts, photosynthesis, water use efficiency, and drought tolerance. traits, 26 structural and 17 functional, associated with photosynthesis and transpiration were quantified on 24 accessions (representatives of 17 species and eight genomes). hypotheses of associations within, and betwee ...201323669746
robustness and strategies of adaptation among farmer varieties of african rice (oryza glaberrima) and asian rice (oryza sativa) across west africa.this study offers evidence of the robustness of farmer rice varieties (oryza glaberrima and o. sativa) in west africa. our experiments in five west african countries showed that farmer varieties were tolerant of sub-optimal conditions, but employed a range of strategies to cope with stress. varieties belonging to the species oryza glaberrima - solely the product of farmer agency - were the most successful in adapting to a range of adverse conditions. some of the farmer selections from within the ...201323536754
a wuschel-related homeobox 3b gene, depilous (dep), confers glabrousness of rice leaves and glumes.glabrousness is an important agricultural trait for the practical breeding of rice. in this study, depilous (dep), the gene responsible for glabrous leaves and glumes of rice was identified by map-based cloning.201227234246
the rice genome knowledgebase (rgkbase): an annotation database for rice comparative genomics and evolutionary biology.over the past 10 years, genomes of cultivated rice cultivars and their wild counterparts have been sequenced although most efforts are focused on genome assembly and annotation of two major cultivated rice (oryza sativa l.) subspecies, 93-11 (indica) and nipponbare (japonica). to integrate information from genome assemblies and annotations for better analysis and application, we now introduce a comparative rice genome database, the rice genome knowledgebase (rgkbase, http://rgkbase.big.ac.cn/rgk ...201323193278
agro-morphological characterization of a population of introgression lines derived from crosses between ir 64 (oryza sativa indica) and tog 5681 (oryza glaberrima) for drought tolerance.the study evaluated effects of drought on some agro-morphological traits of 60 rice genotypes comprising 54 introgression lines with their parents, ir 64 (oryza sativa) and tog 5681 (oryza glaberrima) and four nerica-l varieties developed from the same parents for comparison. the genotypes were subjected either to full irrigation from sowing to maturity (control) or to 21-day drought applied by stopping irrigation from the 45th day after sowing (das) onward (drought) in the dry seasons of 2006 a ...201222195579
conservation and purifying selection of transcribed genes located in a rice centromere.recombination is strongly suppressed in centromeric regions. in chromosomal regions with suppressed recombination, deleterious mutations can easily accumulate and cause degeneration of genes and genomes. surprisingly, the centromere of chromosome8 (cen8) of rice (oryza sativa) contains several transcribed genes. however, it remains unclear as to what selective forces drive the evolution and existence of transcribed genes in cen8. sequencing of orthologous cen8 regions from two additional oryza s ...201121856794
heading date gene, dth3 controlled late flowering in o. glaberrima steud. by down-regulating ehd1.heading date in rice is an important agronomic trait controlled by several genes. in this study, flowering time of variety dianjingyou 1 (djy1) was earlier than a near-isogenic line (named nil) carried chromosome segment from african rice on chromosome 3s, when grown in both long-day (ld) and short-day (sd) conditions. by analyzing a large f2 population from nil × djy1, the locus dth3 (qtl for days to heading on chromosome 3) controlling early heading date in djy1 was fine mapped to a 64-kb segm ...201121830130
patterns of sequence divergence and evolution of the s orthologous regions between asian and african cultivated rice species.a strong postzygotic reproductive barrier separates the recently diverged asian and african cultivated rice species, oryza sativa and o. glaberrima. recently a model of genetic incompatibilities between three adjacent loci: s(1)a, s(1) and s(1)b (called together the s(1) regions) interacting epistatically, was postulated to cause the allelic elimination of female gametes in interspecific hybrids. two candidate factors for the s(1) locus (including a putative f-box gene) were proposed, but candid ...201121423767
genetic diversity and domestication history of african rice (oryza glaberrima) as inferred from multiple gene sequences.nucleotide variation in 14 unlinked nuclear genes was investigated in species-wide samples of african rice (oryza glaberrima) and its wild progenitor (o. barthii). average estimates of nucleotide diversity were extremely low in both species (θ (sil) = 0.0007 for o. glaberrima; θ (sil) = 0.0024 for o. barthii). about 70% less diversity was found in o. glaberrima than in its progenitor o. barthii. coalescent simulation indicated that such dramatic reduction of nucleotide diversity in african rice ...201121400109
exceptional lability of a genomic complex in rice and its close relatives revealed by interspecific and intraspecific comparison and population analysis.extensive dna rearrangement of genic colinearity, as revealed by comparison of orthologous genomic regions, has been shown to be a general concept describing evolutionary dynamics of plant genomes. however, the nature, timing, lineages and adaptation of local genomic rearrangement in closely related species (e.g., within a genus) and haplotype variation of genomic rearrangement within populations have not been well documented.201121385395
distinct evolutionary patterns of oryza glaberrima deciphered by genome sequencing and comparative analysis.here we present the genomic sequence of the african cultivated rice, oryza glaberrima, and compare these data with the genome sequence of asian cultivated rice, oryza sativa. we obtained gene-enriched sequences of o. glaberrima that correspond to about 25% of the gene regions of the o. sativa (japonica) genome by methylation filtration and subtractive hybridization of repetitive sequences. while patterns of amino acid changes did not differ between the two species in terms of the biochemical pro ...201121323774
the molecular basis of white pericarps in african domesticated rice: novel mutations at the rc gene.repeated phenotypic evolution can occur at both the inter- and intraspecific level and is especially prominent in domesticated plants, where artificial selection has favoured the same traits in many different species and varieties. the question of whether repeated evolution reflects changes at the same or different genes in each lineage can now be addressed using the domestication and improvement genes that have been identified in a variety of crops. here, we document the genetic basis of nonpig ...201021121088
paleogenomic analysis of the short arm of chromosome 3 reveals the history of the african and asian progenitors of cultivated rices.rice is one of the most important crops, feeding more than half of the world population. there are two cultivated species, the african rice oryza glaberrima and the asian rice o. sativa. although the african species is gradually replaced by o. sativa in most of african rice agrosystems, this species represents an important reservoir of genes of agronomical interest. their exploitation for the development of modern african rice varieties requires a good understanding of the genetic relationships ...201020333229
the subtelomere of oryza sativa chromosome 3 short arm as a hot bed of new gene origination in rice.despite general observations of non-random genomic distribution of new genes, it is unclear whether or not new genes preferentially occur in certain genomic regions driven by related molecular mechanisms. using 1.5 mb of genomic sequences from short arms of chromosome 3 of oryza glaberrima and o. punctata, we conducted a comparative genomic analysis with the reference o. sativa ssp. japonica genome. we identified a 60-kb segment located in the middle of the subtelomeric region of chromosome 3, w ...200819825586
fine mapping of spr3, a locus for spreading panicle from african cultivated rice (oryza glaberrima steud.).a cssl (chromosome segment substitution line), sg-64, carrying a segment of chromosome 4 from african cultivated rice (cg-14) in the genetic background of var. wuyujing-7 (japonica), showed a spreading panicle, which was different significantly from that of wuyujing-7 with an erect compact panicle. the gene controlling a spreading panicle is referred to as spr3, and is mapped on chromosome 4. to uncover the genetic basis of spr3, a large f(2) population derived from cross between sg-64 and wuyuj ...200819825585
evidence for the emergence of new rice types of interspecific hybrid origin in west african farmers' fields.in west africa two rice species (oryza glaberrima steud. and oryza sativa l.) co-exist. although originally it was thought that interspecific hybridization is impossible without biotechnological methods, progenies of hybridization appear to occur in farmer fields. aflp analysis was used to assess genetic diversity in west africa (including the countries the gambia, senegal, guinea bissau, guinea conakry, sierra leone, ghana and togo) using 315 rice samples morphologically classified prior to ana ...200919806197
contrasting physiological responses by cultivars of oryza sativa and o. glaberrima to prolonged submergence.oryza glaberrima is widely grown in flood-prone areas of african river basins and is subject to prolonged periods of annual submergence. the effects of submergence on shoot elongation, shoot biomass, leaf area and co(2) uptake were studied and compared with those of o. sativa.200918940851
african rice in the columbian exchange. 200118551802
grain qualities and their genetic derivation of 7 new rice for africa (nerica) varieties.nerica are interspecific rice varieties from crossing between the high-yielding asian rice ( oryza sativa spp. japonica) with locally adapted african rice ( oryza glaberrima). in this study, we analyzed grain qualities of 7 nerica varieties (nerica 1 to 7) and genetic derivation of quality-related genes. quality analyses of nerica grains showed that 7 nerica varieties were clearly classified into two groups based on the difference of amylose content, and the difference influenced the pasting and ...200818512938
sex-independent transmission ratio distortion system responsible for reproductive barriers between asian and african rice species.* a sex-independent transmission ratio distortion (sitrd) system detected in the interspecific cross in rice was analyzed in order to understand its significance in reproductive barriers. the s(1) gene, derived from african rice oryza glaberrima, induced preferential abortion of both male and female gametes possessing its allelic alternative (), from asian rice o. sativa, only in the heterozygote. * the sitrd was characterized by resolving it into mtrd and ftrd occurring through male and female ...200818507773
transposable element distribution, abundance and role in genome size variation in the genus oryza.the genus oryza is composed of 10 distinct genome types, 6 diploid and 4 polyploid, and includes the world's most important food crop - rice (oryza sativa [aa]). genome size variation in the oryza is more than 3-fold and ranges from 357 mbp in oryza glaberrima [aa] to 1283 mbp in the polyploid oryza ridleyi [hhjj]. because repetitive elements are known to play a significant role in genome size variation, we constructed random sheared small insert genomic libraries from 12 representative oryza sp ...200717727727
influence of growth temperature on the amounts of tocopherols, tocotrienols, and gamma-oryzanol in brown rice.brown rice is a valuable source of lipid-soluble antioxidants including ferulated phytosterols (i.e., gamma-oryzanol), tocopherols, and tocotrienols. to evaluate the impact of temperature on the accumulation of these compounds, seeds from six different rice lines grown to maturity in replicate greenhouses in gainesville, fl, were analyzed. the lines represented oryza sativa indica, o. sativa japonica, and oryza glaberrima of different origins. temperatures were maintained near ambient at one end ...200717725318
the complex history of the domestication of rice.rice has been found in archaeological sites dating to 8000 bc, although the date of rice domestication is a matter of continuing debate. two species of domesticated rice, oryza sativa (asian) and oryza glaberrima (african) are grown globally. numerous traits separate wild and domesticated rices including changes in: pericarp colour, dormancy, shattering, panicle architecture, tiller number, mating type and number and size of seeds.200717617555
the population structure of african cultivated rice oryza glaberrima (steud.): evidence for elevated levels of linkage disequilibrium caused by admixture with o. sativa and ecological adaptation.genome-wide linkage disequilibrium (ld) was investigated for 198 accessions of oryza glaberrima using 93 nuclear microsatellite markers. significantly elevated levels of ld were detected, even among distantly located markers. free recombination among loci at the population genetic level was shown (1) by a lack of decay in ld among markers on the same chromosome and (2) by a strictly increasing composite likelihood function for the recombination parameter. this suggested that the elevation in ld ...200515545652
qtl detection for rice grain quality traits using an interspecific backcross population derived from cultivated asian (o. sativa l.) and african (o. glaberrima s.) rice.an interspecific advanced backcross population derived from a cross between oryza sativa "v20a" (a popular male-sterile line used in chinese rice hybrids) and oryza glaberrima (accession irgc no. 103544 from mali) was used to identify quantitative trait loci (qtl) associated with grain quality and grain morphology. a total of 308 bc3f1 hybrid families were evaluated for 16 grain-related traits under field conditions in changsha, china, and the same families were evaluated for rflp and ssr marker ...200415284874
rapid recent growth and divergence of rice nuclear genomes.by employing the nuclear dna of the african rice oryza glaberrima as a reference genome, the timing, natures, mechanisms, and specificities of recent sequence evolution in the indica and japonica subspecies of oryza sativa were identified. the data indicate that the genome sizes of both indica and japonica have increased substantially, >2% and >6%, respectively, since their divergence from a common ancestor, mainly because of the amplification of ltr-retrotransposons. however, losses of all clas ...200415240870
assessing hybrid sterility in oryza glaberrima x o. sativa hybrid progenies by pcr marker analysis and crossing with wide compatibility varieties.interspecific crossing of the african indigenous rice oryza glaberrima with oryza sativa cultivars is hindered by crossing barriers causing 100% spikelet sterility in f(1) hybrids. since hybrids are partially female fertile, fertility can be restored by back crossing (bc) to a recurrent male parent. distinct genetic models on spikelet sterility have been developed predicting, e.g., the existence of a gamete eliminator and/or a pollen killer. linkage of sterility to the waxy starch synthase gene ...200312851767
the potential of intermittent irrigation for increasing rice yields, lowering water consumption, reducing methane emissions, and controlling malaria in african rice fields.rice production in sub-saharan africa has more than doubled in the last 3 decades and the potential to further develop rice-harvested areas is considerable. several studies have demonstrated that the transformation of arable land into rice irrigation might create suitable habitats for large populations of disease vectors. prominent among those are anopheline mosquitoes responsible for transmission of malaria. the method of irrigation on an intermittent basis during the rice-cropping calendar has ...200212542191
african rice (oryza glaberrima): history and future potential.the african species of rice (oryza glaberrima) was cultivated long before europeans arrived in the continent. at present, o. glaberrima is being replaced by the introduced asian species of rice, oryza sativa. some west african farmers, including the jola of southern senegal, still grow african rice for use in ritual contexts. the two species of rice have recently been crossed, producing a promising hybrid.200212461173
transcriptome population genomics reveals severe bottleneck and domestication cost in the african rice (oryza glaberrima).the african cultivated rice (oryza glaberrima) was domesticated in west africa 3000 years ago. although less cultivated than the asian rice (o. sativa), o. glaberrima landraces often display interesting adaptation to rustic environment (e.g. drought). here, using rna-seq technology, we were able to compare more than 12,000 transcripts between 9 o. glaberrima, 10 wild o. barthii and one o. meridionalis individuals. with a synonymous nucleotide diversity πs = 0.0006 per site, o. glaberrima appears ...201424684265
cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies.biodiversity, phylogeography and population genetic studies will be revolutionized by access to large data sets thanks to next-generation sequencing methods. in this study, we develop an easy and cost-effective protocol for in-solution enrichment hybridization capture of complete chloroplast genomes applicable at deep-multiplexed levels. the protocol uses cheap in-house species-specific probes developed via long-range pcr of the entire chloroplast. barcoded libraries are constructed, and in-solu ...201424690362
qtl mapping in three rice populations uncovers major genomic regions associated with african rice gall midge resistance.african rice gall midge (afrgm) is one of the most destructive pests of irrigated and lowland african ecologies. this study aimed to identify the quantitative trait loci (qtl) associated with afrgm pest incidence and resistance in three independent bi-parental rice populations (ita306xbw348-1, ita306xtog7106 and ita306xtos14519), and to conduct meta qtl (mqtl) analysis to explore whether any genomic regions are conserved across different genetic backgrounds. composite interval mapping (cim) cond ...201627508500
a proteomic analysis of salt stress response in seedlings of two african rice cultivars.salt stress is one of the key abiotic stresses threatening future agricultural production and natural ecosystems. this study investigates the salt stress response of two rice seedlings, which were screened from 28 kenya rice cultivars. a proteomic analysis was carried out and mapman bin codes employed in protein function categorization. proteins in the redox, stress, and signaling categories were identified, and whose expression differed between the salt tolerant and the salt sensitive samples e ...201627544640
Displaying items 1 - 100 of 125