protein quality in cereals and pulses. 1. application of microbiological and other in vitro methods in the evaluation of rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.).1. the streptococcus zymogenes assay procedure was modified and used in the estimation of available methionine and relative nutritional value in rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). the results were assessed in relation to the content of tannins and 'dye-binding lysine', and to published information on the nutritional quality of some of the test samples. 2. in grain of ten varieties of sorghum, for which other workers had reported a cor ...1979427087
herbimycin, a new antibiotic produced by a strain of streptomyces.herbimycin, a new antibiotic, was isolated from the fermentation broth of streptomyces hygroscopicus strain no. am-3672, a soil isolate. the molecular formula of herbimycin was determined to be c30h42n2o9. herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against cyperus microiria steud. however, oryza sativa showed strong resistance to herbimycin.1979468713
[bean and rice mixtures of high protein value (author's transl)].the optimum content of different rice (oryza sativa) and bean (vigna unguiculata l.) combinations was studied in albino rats. the mixture containing 3 g of rice proteins, corresponding to 41g of rice "in natura", and 7 g of bean protein, corresponding to 59 g of bean "in natura", presented the highest protein efficiency ratio and the feed efficiency ratio. since the limiting amino acid of this mixture was methionine, new assays using varying levels of this amino acid as a supplement were carried ...1979515465
production of 9-beta-d-arabinofuranosyladenine by a new species of streptomyces and its herbicidal activity.9-beta-d-arabinofuranosyladenine (ara-a) was produced by a new species of streptomyces designated as s. herbaceus. ara-a was found to have potent herbicidal activity against echinochloa crus-galli, digitaria adscendens and chenopodium ficifolium by the treatment with ara-a before germination of these plants. however, oryza sativa had strong resistance to ara-a.1979528365
mutagenic effect of argemone oil in oryza sativa view of the large scale adulteration of edible oils with argemone oil, a study was undertaken to test its mutagenicity on rice seed. in treatment with screw-pressed oil a marked reduction in seed germination and high chlorophyll mutation and mutant frequencies were observed. in sequential treatments between gamma rays and argemone oil both mutation and mutant frequencies were significantly higher than that of individual treatments. this study clearly indicates that argemone oil is a potent mu ...1977876267
volatile components of unprocessed rice (oryza sativa l.). 19761002944
the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.).the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens.19751170015
molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.).two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ...19921302636
characterization of a plant sine, p-sine1, in rice genomes.we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ...19921326298
purification and characterization of rice peroxidases.four peroxidase components, named rp-2, 4, 6, and 7, were isolated from rice (oryza sativa l.) green leaves. isoelectric focusing indicated that each preparation was homogeneous. the molecular weights of rp-2, 4, 6, and 7 estimated by sds-page were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. the activity of every preparation was maximum around ph 5.0. antisera against these purified enzymes were raised in rabbits. ouchterlony double ...19911368754
isolation and characterization of cdna clones encoding cdc2 homologues from oryza sativa: a functional homologue and cognate variants.using probes obtained by pcr amplification, we have isolated two cognate rice cdnas (cdc2os-1 and cdc2os-2) encoding structural homologues of the cdc2+/cdc28 (cdc2) protein kinase from a cdna library prepared from cultured rice cells. comparison of the deduced amino acid sequences of cdc2os-1 and cdc2os-2 showed that they are 83% identical. they are 62% identical to cdc28 of saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice r2, a cdc2-related ...19921376401
efficiency of the 5'-terminal sequence (omega) of tobacco mosaic virus rna for the initiation of eukaryotic gene translation in escherichia coli.recent studies have demonstrated that the 5' leader (omega sequence) of tobacco mosaic virus rna has a certain enhancing capacity for translation of mrna in both prokaryotes and eukaryotes. in order to estimate the efficiency of omega to initiate translation of mrna in escherichia coli, in comparison to the shine-dalgarno (s/d) sequence, we have inserted eight different eukaryotic genes into two types of e. coli expression vectors containing one constitutive promoter (p1) but different translati ...19921396694
co-transcription of orf25 and coxiii in rice mitochondria.southern hybridization analysis using homologous maize probes indicated that orf25 and coxiii are closely linked in the mitochondrial genome of rice (oryza sativa) cultivar ir36. the two coding regions were found on the same 5.1 kb bamhi fragment, and this fragment was cloned, mapped and partially sequenced. using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mrna transcript was found containing both orf25 and coxiii coding regions. multiple 5' ends were identified by pr ...19921617739
a novel ras-related rgp1 gene encoding a gtp-binding protein has reduced expression in 5-azacytidine-induced dwarf rice.exposure of normal, tall rice (oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of dna methylation in vivo, induced both demethylation of genomic dna and dwarf plants. genes that had been affected by treatment were identified by differential screening of a cdna library, and a ras-related gene, rgp1, was subsequently isolated. the cdna of rgp1 was found to encode a deduced protein sequence of 226 amino acids with a relative molecular mass of 24850, which was most closely related to ...19911715972
identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide.chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ...19921740436
dietary exposure to aflatoxin in benin city, nigeria: a possible public health concern.a total of 100 samples of various foods comprising of 10 samples each of garri (manihot utilis pohl), beans (phaseolus lunatus), yam flour (dioscorea rotundata), cassava flour (manihot esculentum), melon (citrulus lunatus), onion (allium cepa), rice (oryza sativa), plantain (musa paradisiaca), red pepper (capsicum annuum l., solanaceae) and eggs were screened for the presence of aflatoxins. fifty percent of yam flour; 40% of cassava flour; 30% of garri; 20% of beans and melon and 10% of rice yie ...19911777386
organ-dependent regulation of a plant promoter isolated from rice by 'promoter-trapping' in tobacco.a vector containing a transcriptionally inactive neomycin phosphotransferase ii gene was used to select promoter sequences from a pool of random genomic dna fragments. this paper describes how one such sequence (p4.7) isolated from oryza sativa acts as a hormonally regulated promoter in nicotiana tabacum. relative expression ratios in leaf, root, midrib, callus, and stem tissue of tobacco plants are 1:5:4:10:17. histochemical assays show that p4.7 activates the uida reporter gene throughout the ...19911844878
a homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex i is encoded in chloroplast genomes.the chloroplast genomes of marchantia polymorpha, nicotiana tabacum, and oryza sativa contain open reading frames (orfs or potential genes) encoding homologues of some of the subunits of mitochondrial nadh:ubiquinone oxidoreductase (complex i). seven of these subunits (nd1-nd4, nd4l, nd5, and nd6) are products of the mitochondrial genome, and two others (the 49- and 30-kda components of the iron-sulfur protein fraction) are nuclear gene products. these findings have been taken to indicate the pr ...19911901022
isolation and characterization of a rice gene encoding a basic chitinase.chitinase, which catalyzes the hydrolysis of the beta-1,4-n-acetyl-d-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. a basic chitinase genomic sequence was isolated from a rice (oryza sativa l.) genomic library using a bean chitinase gene fragment as a probe. the complete nucleotide sequence of the rice chitinase rch10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acid ...19912034221
nucleotide sequence of citrus limon 26s rrna gene and secondary structure model of its rna.the complete nucleotide sequence of citrus limon 26s rdna has been determined. the sequence has been aligned with large ribosomal rna (l-rrna) sequences of escherichia coli, saccharomyces cerevisiae and oryza sativa. nine extensive expansion segments in dicot 26s rrna relative to e. coli 23s rrna have been identified and compared with analogous segments of monocot, yeast, amphibian and human l-rrnas. a secondary structure model for lemon 26s rrna has been derived based on the refined model of e. ...19902101688
recognition efficiency of dicotyledoneae-specific promoter and rna processing signals in rice.heterologous gene expression experiments have shown that genes of monocotyledoneae are often not transcribed in dicotyledoneae, or produce pre-mrna that is inefficiently or aberrantly processed. it is however not known how correctly and efficiently dicotyledon-specific gene expression signals are recognized in cells of monocotyledoneae. here we address this question using tobacco (nicotiana tabacum) and rice (oryza sativa) protoplasts transformed with the same hybrid gene constructs. constructs ...19902177137
fingerprinting genomes using pcr with arbitrary primers.simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (pcr). no prior sequence information is required. the method, arbitrarily primed pcr (ap-pcr), involves two cycles of low stringency amplification followed by pcr at higher stringency. we show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. the generality of the method is demonstrated by application to twenty four ...19902259619
[larval density of anopheles (n) albimanus wiedemann, 1821 in relation to phenology of oryza sativa (linnaeus) rice].the larval density of anopheles (n) albimanus wiedemann, 1821, is linked with rice phenology in the two crops of the year (summer and winter). it was determined that a higher density agrees with the second stage of the vegetative cycle of the culture named beginning of shooting processes and the formation of the panicular primordium. the number of anopheline larvae was higher in the summer.19892672175
the complete sequence of the rice (oryza sativa) chloroplast genome: intermolecular recombination between distinct trna genes accounts for a major plastid dna inversion during the evolution of the cereals.the entire chloroplast genome of the monocot rice (oryza sativa) has been sequenced and comprises 134525 bp. predicted genes have been identified along with open reading frames (orfs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (nicotiana tabacum), and a liverwort (marchantia polymorpha). the same complement of 30 trna and 4 rrna genes has been conserved between rice and tobacco. most orfs extensively conserved between n. tabacum and m. polymorpha ar ...19892770692
the effects of fruit juices and fruits on the absorption of iron from a rice meal.the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...19873593665
leaf surface fungi of oryza sativa linn. 19715105623
effect of water washing, lime treatment and lime and calcium carbonate supplementation on the nutritive value of paddy (oryza sativa) straw. 19695818438
[evaluation of nutritional potential of fish in diets based on beans (phaseolus vulgaris) and a cereal/corn (zea mays) and/or rice (oryza sativa)].p6e complementation between corn and fish, and rice and fish was evaluated for the purpose of establishing the required levels of each constituent in the mixture to obtain the optimum value in the biological evaluations for each diet. the optimum fish levels were around 10 and 6% for the corn and rice diets, respectively. complementary levels of fish in the bean: corn and bean:rice diets were evaluated in the same manner. the best values in the biological evaluation were obtained with only 2% of ...19836675550
a novel lipoxygenase from rice. primary structure and specific expression upon incompatible infection with rice blast fungus.a novel lipoxygenase cdna (3,007 base pairs) was isolated from rice leaves (oryza sativa cv. aichiasahi) which had been infected with an incompatible race of the rice blast fungus, magnaporthe grisea. a single copy of the gene is present in the rice genome and encodes a protein of 923 residues with a molecular weight of 102,714. this gene product shares the least amino acid sequence homology among plant lipoxygenases identified to date. a novel feature of this gene product is a putative transit ...19947508918
identification, cdna cloning, and gene expression of soluble starch synthase in rice (oryza sativa l.) immature seeds.three forms of soluble starch synthase were resolved by anion-exchange chromatography of soluble extracts from immature rice (oryza sativa l.) seeds, and each of these forms was further purified by affinity chromatograph. the 55-, 57-, and 57-kd proteins in the three preparations were identified as candidates for soluble starch synthase by western blot analysis using an antiserum against rice granule-bound starch synthase. it is interesting that the amino-terminal amino acid sequence was identic ...19937518089
2,3-bisphosphoglycerate-independent phosphoglycerate mutase is conserved among different phylogenic kingdoms.we have previously demonstrated that maize (zea mays) 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgam-i) is not related to 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase. with the aid of specific anti-maize pgam-i antibodies, we demonstrate here the presence of a closely related pgam-i in other plants. we also describe the isolation and sequencing of a cdna-encoding almond (prunus amygdalus) pgam-i that further demonstrates this relationship among plant pgam-i. a sea ...19957584858
the regulatory regions of the rice tungro bacilliform virus promoter and interacting nuclear factors in rice (oryza sativa l.).the rice tungro bacilliform virus (rtbv) promoter confers phloem-specific gene expression in transgenic rice plants. a series of promoter deletion mutants were fused with the escherichia coli beta-glucuronidase a (uida) reporter gene and introduced into transgenic rice plants. the rtbv promoter confers substantially stronger expression in shoots than in roots. a fragment of the promoter comprising nucleotides -164 to +45 relative to the transcriptional start site contains sufficient information ...19957599653
a view of plant dehydrins using antibodies specific to the carboxy terminal peptide.dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ...19937693020
proline accumulates in plants exposed to uv radiation and protects them against uv induced peroxidation.proline accumulated in the shoots of seedlings of rice (oryza sativa), mustard (brassica juncea) and mung bean (vigna radiata) exposed to uv radiations. the level of proline in the seedlings increased significantly with increase in uv exposure time. the production of malondialdehyde (an indice of lipid peroxidation) was also higher in the shoots of seedlings exposed to uv radiation as compared to controls, suggesting that uv radiations promote lipid peroxidation. the extent of uv radiation promo ...19957726821
the atx1 gene of saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as superoxide dismutase (sod) and catalase. here we describe the isolation and characterization of another gene in the yeast saccharomyces cerevisiae that plays a critical role in detoxification of reactive oxygen species. this gene, named atx1, was originally isolated by its ability to suppress oxygen toxicity in yeast lacking sod. atx1 encodes a 8.2-kda polypep ...19957731983
six new candidate members of the alpha/beta twisted open-sheet family detected by sequence similarity to flavodoxin.strong sequence similarity has been reported among wrba (the trp repressor-binding protein of escherichia coli); ycp4, a protein of unknown function from the budding yeast saccharomyces cerevisiae; p25, the pap1-dependent protein of the fission yeast schizosaccharomyces pombe; and the translation product of a partial cdna sequence from rice seedling root (oryza sativa, locus ricr02421a; here referred to as ricr). further homology search with the profile method indicates that all the above sequen ...19947756978
heat-inducible rice hsp82 and hsp70 are not always co-regulated.we have characterized several heat-shock-induced genes in rice (oryza sativa l.) and compared their expression under a variety of conditions. three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. the middle member of this cluster, designated hsp82b, has been fully sequenced. the gene uses a promoter containing six putative heat-shock elements as well as several unusual sequence motifs including a stretch of 11 thymidines altern ...19947764623
thioredoxin h is one of the major proteins in rice phloem sap.sieve tubes play important roles in the transfer of nutrients as well as signals. hundreds of proteins were found in pure phloem sap collected from rice (oryza sativa l. cv. kantou) plants through the cut ends of insect stylets. these proteins may be involved in nutrient transfer and signal transduction. to characterize the nature of these proteins, the partial amino-acid sequence of a 13-kda protein, named rpp13-1, that was abundant in the pure phleom sap was determined. a cdna clone of 687 bp, ...19957766047
expression of functional proteins of cdna encoding rice nucleoside diphosphate kinase (ndk) in escherichia coli and organ-related alteration of ndk activities during rice seed germination (oryza sativa l.).the gst (glutathione s-transferase)-ndk (nucleoside diphosphate kinase) fusion protein was expressed in escherichia coli. the gst-ndk protein was capable of transferring gamma-phosphate from atp to nucleoside diphosphates such as gdp, cdp, tdp and udp. western blot analysis using anti-ndk antibody indicated that ndk in endosperm gradually decreased during 36 h of imbibition. on the contrary, ndk in embryo increased during the same period. ndk activities in both tissues were in accord with these ...19957766875
metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.fluorescence in situ hybridization (fish) is a powerful tool for physical mapping in human and other mammalian species. however, application of the fish technique has been limited in plant species, especially for mapping single- or low-copy dna sequences, due to inconsistent signal production in plant chromosome preparations. here we demonstrate that bacterial artificial chromosome (bac) clones can be mapped readily on rice (oryza sativa l.) chromosomes by fish. repetitive dna sequences in bac c ...19957753830
structure and differential response to abscisic acid of two promoters for the cytosolic copper/zinc-superoxide dismutase genes, sodcc1 and sodcc2, in rice protoplasts.we determined the 5'-flanking sequences of two nuclear genes (sodcc1 and sodcc2) encoding cytosolic copper/zinc-superoxide dismutase in rice (oryza sativa l.). utilizing transient beta-glucuronidase (gus) reporter assays, functional promoter-gus analysis was performed in rice protoplasts exposed to the phytohormone abscisic acid (aba) or the antioxidant sulfhydryl reagent, dithiothreitol (dtt). transcriptional activities from both sodcc-gus fusions were stimulated by dtt, which induces the promo ...19957821431
fractionation and characterization of two forms of peroxidase from oryza sativa.peroxidase (e.c., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ...19957603969
an amino-terminal deletion of rice phytochrome a results in a dominant negative suppression of tobacco phytochrome a activity in transgenic tobacco seedlings.overexpression of phytochrome a results in an increased inhibition of hypocotyl elongation under red and far-red light. we used this approach to assay for the function of n-terminal mutations of rice (oryza sativa l.) phytochrome a. transgenic tobacco seedlings that express the wild-type rice phytochrome a (rw), a rice phytochrome a lacking the first 80 amino acids (ntd) or a rice phytochrome a with a conversion of the first 10 serines into alanine residues (s/a) were compared with untransformed ...19957580859
rice scutellum induces agrobacterium tumefaciens vir genes and t-strand generation.for successful transformation of a plant by agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce agrobacterium tumour-inducing (ti) plasmid virulence (vir) genes. here we report a significant variation in different tissues of indica rice (oryza sativa l. cv. co43) in their ability to induce agrobacterium tumefaciens vir genes and t-strand generation, using explants preincubated in liquid murashige and skoog (ms) medium. an analysis of rice le ...19957579158
palindromic repeated sequences (prss) in the mitochondrial genome of rice: evidence for their insertion after divergence of the genus oryza from the other gramineae.we have identified a family of small repeated sequences (from 60 to 66 bp in length) in the mitochondrial genome of rice (oryza sativa cv. nipponbare). there are at least ten copies of these sequences and they are distributed throughout the mitochondrial genome. each is potentially capable of forming a stem-and-loop structure and we have designated them prss (palindromic repeated sequences). their features are reminiscent of the small dispersed repeats in the mitochondrial dna (mtdna) of some lo ...19947509206
expression of functional proliferating-cell nuclear antigen from rice (oryza sativa) in escherichia coli. activity in association with human dna polymerase delta.proliferating-cell nuclear antigen (pcna), the auxiliary protein for dna polymerase delta, is one of the key factors for both pcna-dependent dna synthesis and cell-cycle progression. plant pcna genes have previously been cloned from rice, carrot, tobacco, and soybean cells by screening the cdna libraries using similarity to the human or rat pcna genes. we subcloned the relevant gene from the rice pcna cdna into an escherichia coli expression vector pmal, and the pcna protein was expressed in the ...19947913441
efficient transformation of rice (oryza sativa l.) mediated by agrobacterium and sequence analysis of the boundaries of the t-dna.a large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with agrobacterium tumefaciens. the efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the r0, r1 and r2 generations. sequence analysis revealed that the ...19947920717
a comparative study of cadmium, lead, zinc, ph, and bulk density from the enyigba lead and zinc mine in two different seasons.soil ph and bulk density, as well as the concentrations of cadmium (cd), lead (pb), and zinc (zn) in soils and these metal levels in the leaves of cassava (manihot esculenta) and rice (oryza sativa), both collected, respectively, in the dry season (december) of 1991 and the rainy season (september) of 1992 from the enyigba lead and zinc mine, nigeria, were investigated. interspecific variations in trace element levels in the plant leaves were detected and may reflect the predilection of these pl ...19957498063
nramp defines a family of membrane proteins.nramp (natural resistance-associated macrophage protein) is a newly identified family of integral membrane proteins whose biochemical function is unknown. we report on the identification of nramp homologs from the fly drosophila melanogaster, the plant oryza sativa, and the yeast saccharomyces cerevisiae. optimal alignment of protein sequences required insertion of very few gaps and revealed remarkable sequence identity of 28% (yeast), 40% (plant), and 55% (fly) with the mammalian proteins (46%, ...19957479731
molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice.a receptor-like protein kinase, ospk10, has been cloned from rice (oryza sativa). the 2.8 kb cdna contains an open reading frame capable of encoding a peptide sequence of 824 amino acids. the topological features of the predicted ospk10 protein include an n-terminal signal peptide, a cysteine-rich extracellular ligand-binding domain, a membrane-spanning segment, and a cytoplasmic domain possessing all the hallmarks of catalytic domains of eukaryotic protein kinases. the cytoplasmic domain was se ...19947999995
active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases.with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ...19826765191
cloning and structural analysis of calmodulin gene from rice.full-length cdna encoding rice (oryza sativa, cv. nongken 58s) calmodulin (cam) mrna has been cloned using polymerase chain reaction (pcr) with synthesized primers from the sequence of barley cam gene. sequence analysis revealed that the rice gene is 450 bp in length that and its predicted polypeptide consists of 148 amino acids. it differs from those that of barley and alfalfa in only one position, respectively. the nucleotide homology between rice and barley is 90%, between rice and alfalfa is ...19938061233
rice tungro bacilliform virus: transcription and translation in protoplasts.protoplasts from cell suspension cultures of oryza sativa (monocot) and orychophragmus violaceus (dicot) support transcription from the rice tungro bacilliform virus (rtbv) promoter and translation of the resulting mrna despite the presence of a long leader sequence with strong secondary structure and 12 short open reading frames. transcriptional elements located both upstream and downstream of the transcription initiation site are defined by deletion analysis and the functional tata motif is de ...19948091688
isolation, molecular and biological properties of a lectin from rice embryo: relationship with wheat germ agglutinin properties.rice lectin (oryza sativa, var. balilla 28) was purified from defatted embryos by aqueous acid extraction at ph 1.3 followed by ammonium sulfate precipitation between 2 and 4 m, affinity chromatography on agarose-p-aminophenyl-beta-d-n-acetylglucosamine, and gel filtration on aca 54. the homogeneity of the lectin was checked by polyacrylamide gel electrophoresis, gel filtration, and immunodiffusion. the amino acid analysis revealed a high half-cystine content (9%) and a low aromatic and hydropho ...19873688888
a rice (oryza sativa l.) cdna encodes a protein sequence homologous to the eukaryotic ribosomal 5s rna-binding protein.a rice (oryza sativa l.) cdna clone coding for the cytoplasmic ribosomal protein l5, which associates with 5 s rrna for ribosome assembly, was cloned and its nucleotide sequence was determined. the primary structure of rice l5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. these include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three ...19938219074
nascent transcript-binding protein of the pea chloroplast transcriptionally active chromosome.this study describes the nascent rna-binding protein of the pea chloroplast transcriptional complex. the protein has been identified by photoaffinity labelling of the transcriptionally active chromosome (tac) which utilizes the endogenous plastid dna as template. uv irradiation of lysed chloroplast or the isolated tac under conditions optimized for transcription photocross-links nascent radiolabelled transcripts (up to 250 nucleotides in length) to a 48 kda protein. the photoaffinity labelling o ...19938260634
chromosomal locations of ten isozyme loci in rice (oryza sativa l.) through trisomic analysis.chromosomal locations of 10 isozyme loci in rice (oryza sativa l.) were determined through trisomic analysis. all 10 genes produced altered allozyme banding patterns in specific f1 trisomics. this served as the primary source of evidence for chromosome locations of est-5, icd-1, acp-1, and pgd-1. the locations of amp-1, amp-2, amp-4, pox-5, got-1, and cat-1 were further confirmed from segregation data in bc1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but ...19883408480
food-based solutions are a viable alternative to glucose-electrolyte solutions for oral hydration in acute diarrhoea--studies in a rat model of secretory diarrhoea.a survey of acute diarrhoea and its treatment, in 3 groups of villages in south india, revealed that use of the world health organization oral rehydration solution (who-ors) was poor or virtually non-existent and that several liquid foods were given to children during acute diarrhoea. the effects of the most commonly used, boiled and cooled supernatants of these liquid foods [rice (oryza sativa)-water, ragi (eleusine coracana)-water, arrowroot (maranta arundinacea)-water], and tender coconut-wat ...19902345922
cloning and sequence analysis of a cdna encoding rice glutaredoxin.a full-length cdna clone (rasc8) encoding glutaredoxin (thioltransferase) was isolated from a cdna library of an aleurone layer prepared from a developing seed of rice (oryza sativa l.). rasc8, 568bp in length, contained an atg codon and two possible polyadenylation signals, and encoded 112 amino acid residues. cys-pro-phe-cys, which is the active site and a highly conserved sequence among thioltransferases, was found in the deduced amino acid sequence. rasc8 was introduced into an expression ve ...19948287970
a 127 kda component of a uv-damaged dna-binding complex, which is defective in some xeroderma pigmentosum group e patients, is homologous to a slime mold protein.a cdna which encodes a approximately 127 kda uv-damaged dna-binding (uv-ddb) protein with high affinity for (6-4)pyrimidine dimers [abramic', m., levine, a.s. & protic', m., j. biol. chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cdna library. the presence of this protein in complexes bound to uv-damaged dna was confirmed by immunoblotting. the human cognate of the uv-ddb gene was localized to chromosome 11. uv-ddb mrna was expressed in all human tissues examined, including c ...19938371985
anaerobiosis and plant growth hormones induce two genes encoding 1-aminocyclopropane-1-carboxylate synthase in rice (oryza sativa l.).the plant hormone ethylene is believed to be responsible for the ability of rice to grow in the deepwater regions of southeast asia. ethylene production is induced by hypoxia, which is caused by flooding, because of enhanced activity of 1-aminocyclopropane-1-carboxylic acid (acc) synthase, the key enzyme in the ethylene biosynthetic pathway. we have cloned three divergent members, (os-acs1, os-acs2, and os-acs3), of a multigene family encoding acc synthase in rice. os-acs1 resides on chromosome ...19938389618
agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene.we have successfully transferred and expressed a reporter gene driven by an alpha-amylase promoter in a japonica type of rice (oryza sativa l. cv. tainung 62) using the agrobacterium-mediated gene transfer system. immature rice embryos (10-12 days after anthesis) were infected with an agrobacterium strain carrying a plasmid containing chimeric genes of beta-glucuronidase (uida) and neomycin phosphotransferase (nptii). co-incubation of potato suspension culture (psc) with the agrobacterium inocul ...19938392395
cloning and characterization of a cdna encoding an 18.0-kda class-i low-molecular-weight heat-shock protein from rice.a novel cdna clone, oshp18.0 cdna, encoding a rice (oryza sativa l. cv. tainong 67) 18.0-kda heat-shock protein (hsp), was isolated from a cdna library of heat-shocked rice seedlings by use of the rice hsp cdna, oshsp17.3 cdna, as a probe. the sequence showed that oshsp18.0 cdna contains a 749-bp insert encoding an orf of 160 amino acids, with a predicted molecular mass of 18.0 kda and a pi of 7.3. sequence comparison reveals that oshsp18.0 cdna is highly homologous to other low-molecular-weight ...19958522180
cloning and properties of a rice gene encoding phenylalanine ammonia-lyase.phenylalanine ammonia-lyase (pal) genomic sequences were isolated from a rice (oryza sativa l.) genomic library using a pcr-amplified rice pal dna fragment as a probe. there is a small family of pal genes in the rice genome. the nucleotide sequence of one pal gene, zb8, was determined. the zb8 gene is 4660 bp in length and consists of two exons and one intron. it encodes a polypeptide of 710 amino acids. the transcription start site was 137 bp upstream from the translation initiation site. rice ...19958534851
open reading frame 176 in the photosynthesis gene cluster of rhodobacter capsulatus encodes idi, a gene for isopentenyl diphosphate isomerase.isopentenyl diphosphate (ipp) isomerase catalyzes an essential activation step in the isoprenoid biosynthetic pathway. a database search based on probes from the highly conserved regions in three eukaryotic ipp isomerases revealed substantial similarity with orf176 in the photosynthesis gene cluster in rhodobacter capsulatus. the open reading frame was cloned into an escherichia coli expression vector. the encoded 20-kda protein, which was purified in two steps by ion exchange and hydrophobic in ...19968550491
regulation, expression and function of a new basic chitinase gene in rice (oryza sativa l.).a new basic chitinase gene, designated rc24, was isolated from a rice genomic library. the predicted rc24 protein contains 322 amino acid residues and exhibits 68% to 95% amino acid identity with known class i rice chitinases. rc24 protein expressed in escherichia coli exhibited chitinase activity and strongly inhibited bacterial growth. two transcription start sites of the rc24 gene were mapped by primer extension analysis of both rice native rna and in vitro transcribed rna using a rc24 promot ...19968605293
cloning and characterization of the rice cata catalase gene, a homologue of the maize cat3 gene.we isolated and sequenced a genomic clone (cata) encoding cat-a catalase, a homologue of the maize catalase isozyme 3 (cat-3) from rice (oryza sativa l.). the 5'-upstream non-coding region had very low similarity with the maize cat3 gene and possible cis elements and sequence motifs in the maize cat3 gene were not evident, except for tata and caat motifs. several sequence motifs found in the promoters of plant seed-specific genes were identified in the 5'-upstream non-coding region of the cata g ...19968605302
molecular cloning of the full-length cdna of (s)-hydroxynitrile lyase from hevea brasiliensis. functional expression in escherichia coli and saccharomyces cerevisiae and identification of an active site residue.the full-length cdna of (s)-hydroxynitrile lyase (hnl) from leaves of hevea brasiliensis (tropical rubber tree) was cloned by an immunoscreening and sequenced. hnl from h. brasiliensis is involved in the biodegradation of cyanogenic glycosides and also catalyzes the stereospecific synthesis of aliphatic, aromatic, and heterocyclic cyanohydrins, which are important as precursors for pharmaceutical compounds. the open reading frame identified in a 1. 1-kilobase cdna fragment codes for a protein of ...19968621461
a novel cross-phylum family of proteins comprises a krr1 (ycl059c) gene which is essential for viability of saccharomyces cerevisiae cells.we demonstrate here that the open reading frame (orf) ycl059c, discovered during the systematic sequencing of chromosome iii [oliver et al., nature 357 (1992) 38-46], codes for a protein essential for yeast: neither spore germination nor cell division occur in strains deleted for this gene. we have cloned the wild-type (wt) gene and shown that it complements the deletion. a relatively abundant rna transcript corresponds to the gene. the protein has no similarity to proteins of known function. in ...19968675026
magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses.we have identified genes for pathogenicity toward rice (oryza sativa) and genes for virulence toward specific rice cultivars in the plant pathogenic fungus magnaporthe grisea. a genetic cross was conducted between the weeping lovegrass (eragrostis curvula) pathogen 4091-5-8, a highly fertile, hermaphroditic laboratory strain, and the rice pathogen o-135, a poorly fertile, female-sterile field isolate that infects weeping lovegrass as well as rice. a six-generation backcrossing scheme was then un ...19912016048
the drosophila melanogaster gene vha14 encoding a 14-kda f-subunit of the vacuolar atpase.a drosophila melanogaster (dm) cdna (vha14) encoding the 14-kda f-subunit of the vacuolar h(+)-atpase (v-atpase) has been cloned via homology with the corresponding manduca sexta (ms) gene. its deduced translation product is a 124-amino-acid polypeptide sharing 90% identity with the ms polypeptide and 50% identity with an analogous polypeptide of saccharomyces cerevisiae, and a more distant similarity to a subunit of the na(+)-transporting atpase of enterococcus hirae. homology was also found wi ...19968682310
cloning and characterization of repetitive dna sequences from genomes of oryza minuta and oryza australiensis.the value of genome-specific repetitive dna sequences for use as molecular markers in studying genome differentiation was investigated. five repetitive dna sequences from wild species of rice were cloned. four of the clones, pom1, pom4, poma536, and pompb10, were isolated from oryza minuta accession 101141 (bbcc genomes), and one clone, poa237, was isolated from oryza australiensis accession 100882 (ee genome). southern blot hybridization to different rice genomes showed strong hybridization of ...19911955157
vectors carrying two separate t-dnas for co-transformation of higher plants mediated by agrobacterium tumefaciens and segregation of transformants free from selection markers.novel 'super-binary' vectors that carried two separate t-dnas were constructed. one t-dna contained a drug-resistance, selection-marker gene and the other contained a gene for beta-glucuronidase (gus). a large number of tobacco (nicotiana tabacum l.) and rice (oryza sativa l.) transformants were produced by agrobacterium tumefaciens lba4404 that carried the vectors. frequency of co-transformation with the two t-dnas was greater than 47% gus-positive, drug-sensitive progeny were obtained from mor ...19968758986
phosphorylation of a protein (pp56) is related to the regeneration of rice cultured suspension cells.short-term cultured suspension cells of rice (oryza sativa l.) are capable of regeneration, but not in long-term culture. for clarification of the mechanism of regeneration, protein phosphorylation in short-term and long-term cultured suspension cells was compared by two dimensional-polyacrylamide gel electrophoresis. a 56 kda protein having an isoelectric point of 4.5 was phosphorylated in vitro in short-term cultured suspension cells, but was not phosphorylated after regeneration. this protein ...19968888618
transgenic rice cell lines and plants: expression of transferred chimeric genes.cell suspension-derived rice (oryza sativa l.) protoplasts were transformed by direct gene uptake. peg-mediated transformation was more efficient than electroporation. plasmid dna containing a hygromycin phosphotransferase (hpt) gene (which confers hygromycin resistance) driven by the camv 35s promoter and a beta-d-glucuronidase (gus) gene under control of the 1', 2' double promoter of the mannopine synthase (mas) locus of agrobacterium tumefaciens was introduced into rice protoplasts. southern ...19911713479
the isolation and characterisation of plant sequences homologous to human hypervariable minisatellites.we have isolated dna probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (oryza sativa). these probes are capable of producing a multilocus rice dna fingerprint. the rice sequence has a tandem repeating structure based on a 12 bp gc-rich repeat which shows homology to its human counterpart. this probe detects up to 30 loci which are at a number of unlinked chromosomal sites. the gc-rich sequence is invariably associated with an open reading frame ( ...19911651259
factors influencing agrobacterium-mediated transient expression of gusa in rice.transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ...19921463839
[the goal-oriented project "pathology of environmental pollutants" (m.u.r.s.t. 40%)].the purpose of this study was to estimate to toxicity bound to effluent water, pore water and bed sediment sampled twice during 1994 (spring and autumn) from 16 tributaries of po river. toxicity was determined by using a battery of bioassays (artemia salina, dugesiagonocephala and thamnocephalus platyurus) and a battery of phytoassays, including oryza sativa and triticum sativum. results show that practically no toxicity is found in effluent water, mild adverse effects are obtained with sediment ...19958991825
t-dna integration into genomic dna of rice following agrobacterium inoculation of isolated shoot apices.this paper establishes that the isolated shoot meristem of monocotyledons can be infected and transformed using agrobacterium. since this explant from nearly any cereal cultivar can rapidly regenerate into a plant, using this explant effectively eliminates the genotype regeneration restrictions to cereal crop transformation allowing direct transformation of elite germplasm. shoot apices of oryza sativa l. tropical japonica, cv. maybelle were explants used for cocultivation, and gene transfer was ...19969002612
structure, chromosomal location and expression of a rice gene encoding the microsome omega-3 fatty acid desaturase.the omega-3 fatty acid desaturases are membrane-bound enzymes catalyzing the conversion of linoleic acid to linolenic acid in lipids, and are located both in the microsome and plastid envelopes as two different isoforms. a cdna encoding the microsome omega-3 fatty acid desaturase (osfad3) and the corresponding genomic clone were isolated from rice (oryza sativa l.). the osfad3 gene was composed of 8 exons and 7 introns. a microsatellite was present in the second exon of the osfad3 gene, showing ...19979049269
expression, secretion, and processing of rice alpha-amylase in the yeast yarrowia lipolytica.the gene encoding rice alpha-amylase in oryza sativa was expressed in the yeast yarrowia lipolytica, which is a potential host system for heterologous protein expression. for efficient secretion, the strong and inducible xpr2 promoter was used in the construction of four kinds of expression vectors with the following configurations between the xpr2 promoter and terminator: 1) xpr2 prepro-region-rice alpha-amylase coding sequence, 2) rice alpha-amylase signal peptide-rice alpha-amylase coding seq ...19979054373
chloroplast dna variability in wild and cultivated rice (oryza spp.) revealed by polymorphic chloroplast simple sequence repeats.short mononucleotide repeats analogous to nuclear microsatellites or simple sequence repeats (ssrs) have been identified in chloroplast genomes. primers flanking mononucleotide repeats in the fully sequenced rice chloroplast genome have been used in conjunction with pcr to amplify genomic dna from 42 wild rice accessions. the amplification products exhibited length polymorphism, which allowed the levels of chloroplast variability detected to be quantified. seven primer pairs that amplified produ ...19979061917
differential expression of a cak (cdc2-activating kinase)-like protein kinase, cyclins and cdc2 genes from rice during the cell cycle and in response to gibberellin.progress through the eukaryotic cell cycle is regulated by cyclin-dependent cdc2 protein kinases. in rice (oryza sativa l), two cdc2 protein kinases, cdc2os-1 and cdc2os-2, and two cyclins, cycos1 and cycos2, have been described. in this study, we report on the cell-cycle phase-specific expression of these genes. using partially synchronized suspension cells from rice, we found that cdc2os-1 was expressed constitutively throughout the cell cycle. the cdc2os-2 transcript level was elevated in g1 ...19979076986
the root hair defective3 gene encodes an evolutionarily conserved protein with gtp-binding motifs and is required for regulated cell enlargement in plants, morphogenesis is largely determined by the orientation and extent of cell enlargement. to define the molecular mechanisms regulating plant cell enlargement, we have conducted a molecular genetic analysis of the root hair defective3 (rhd3) gene of arabidopsis thaliana. mutations affecting the rhd3 gene were found to alter cell size, but not cell number, in tissues throughout the plant. genetic and physiological analyses suggest that the rhd3 gene is not required for proper cell type sp ...19979087433
structure of the rice glutaredoxin (thioltransferase) gene.we have isolated the gene encoding a glutaredoxin in rice (oryza sativa l.) and determined the nucleotide (nt) sequence of about a 4.2 kb long. the cloned gene (grasc8) was found to contain four exons interrupted by three introns. the first exon begins the atg translation start codon and the four exons code for a protein composed of 112 amino acids. the tetrapeptide -cys-pro-phe-cys- [-cys-pro-phe(tyr)-cys-] which constitutes an active site of escherichia coli and mammalian glutaredoxins, was co ...19979099854
cloning and characterization of the osnramp family from oryza sativa, a new family of membrane proteins possibly implicated in the transport of metal ions.the mammalian nramp1 protein is an integral membrane protein expressed exclusively in macrophages, where it plays a critical role in the ability of these cells to destroy ingested microbes. the bactericidal mechanism of action of nramp1 remains unknown. we report the identification and characterization of cdna clones corresponding to three homologues of the mammalian nramp1 gene from the genome of oryza sativa, osnramp1, osnramp2, and osnramp3. these three genes encode a novel group of highly si ...19979154989
the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase.a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ...19921421143
biochemical characteristics of a rice (oryza sativa l., ir36) g-protein alpha-subunit expressed in escherichia coli.a cdna encoding the alpha-subunit of the heterotrimeric g-protein in rice (rga1) was overexpressed in escherichia coli and then isolated by ni2+-nitrilotriacetic acid affinity chromatography. the molecular mass of rga1 bearing a his tag was approx. 49 kda. immunoblot analysis using anti-rga1 revealed that the rga1 protein is most abundant in seedling leaves and least abundant in mature roots. it exists at particularly high levels in the immature embryo after pellicle extrusion. in addition, the ...19979164867
purification and characterization of glutaredoxin (thioltransferase) from rice (oryza sativa l.).we purified and characterized glutaredoxin (thioltransferase), which catalyzes thiol/disulfide exchange reaction, for the first time in plants. the purification procedure employed an immunoabsorbent, antiglutaredoxin-sepharose. glutaredoxin was purified about 2,200-fold from rice bran and it appeared to be homogeneous on sds-page. maldi-tof mass spectrometry revealed that the protein has a molecular mass of 11,097.9 da. rice glutaredoxin consists of 105 amino acid residues, containing the tetrap ...19979192723
genetic and physical analysis of the rice bacterial blight disease resistance locus, xa21.nearly isogenic lines (nils) of rice (oryza sativa) differing at a locus conferring resistance to the pathogen xanthomonas oryzae pv. oryzae were surveyed with 123 dna markers and 985 random primers using restriction fragment length plymorphism (rflp) and random amplified polymorphic dna (rapd) analysis. one chromosome 11 marker (rg103) detected polymorphism between the nils that cosegregated with xa21. all other chromosome 11 dna markers tested were monomorphic between the nils, localizing the ...19921362973
a large-insert (130 kbp) bacterial artificial chromosome library of the rice blast fungus magnaporthe grisea: genome analysis, contig assembly, and gene cloning.magnaporthe grisea (hebert) barr causes rice blast, one of the most devastating diseases of rice (oryza sativa) worldwide. this fungus is an ideal organism for studying a number of aspects of plant-pathogen interactions, including infection-related morphogenesis, avirulence, and pathogen evolution. to facilitate m. grisea genome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (bac) library from the rice infecting strain 70-15. a new metho ...19979290247
molecular cloning and further characterization of a probable plant vacuolar sorting receptor.bp-80 is a type i integral membrane protein abundant in pea (pisum sativum) clathrin-coated vesicles (ccvs) that binds with high affinity to vacuole-targeting determinants containing asparagine-proline-isoleucine-arginine. here we present results from cdna cloning and studies of its intracellular localization. its sequence and sequences of homologs from arabidopsis, rice (oryza sativa), and maize (zea mays) define a novel family of proteins unique to plants that is highly conserved in both monoc ...19979306690
hydrogen isotope effects in the cyclization of d-glucose 6-phosphate by myo-inositol-1-phosphate synthase.myo-inositol-1-phosphate synthase (ec from rat testis, acer pseudoplatanus l. cell culture and oryza sativa l. cell culture, converted d-[5-3h]glucose 6-phosphate to myo-[2-3h]inositol 1-phosphate at rates ranging from 0.21 to 0.48 that of unlabeled substrate. d-[3-3h]- and d-[4-3h]glucose 6-phosphate were converted at approximately the same rate as that of unlabeled substrate. in the case of testis enzyme, storage as a frozen solution further lowered the rate with d-[5-3h]glucose 6-pho ...1977903359
structure and expression of two cdnas encoding s-adenosyl-l-methionine synthetase of rice (oryza sativa l.).two cdnas encoding rice (oryza sativa l.) s-adenosyl-l-methionine synthetase (sams) have been cloned, sequenced and identified. the deduced protein sequences share a high homology (90-94%) with those of other plant sams and are 60-62% identical to yeast, rat and human sams. the rice sams genes are differentially regulated in a tissue-specific manner and by a salt stress, while they are coordinately expressed during growth of the rice cell culture.19979375784
purification and characterization of a trypsin inhibitor from rice bran.a trypsin inhibitor was isolated and purified from the bran of rice, oryza sativa, by extraction with 1% sodium chloride, heat treatment, ammonium sulfate precipitation, ion-exchange chromatography on a cm-sephadex c-25 and gel filtration on a sephadex g-75. the final preparation was homogeneous by electrophoretic analysis. rice bran trypsin inhibitor (rbti) had a molecular weight of about 14,500 and an isoelectric point of 8.07. the amino acids, acid composition was characterized by high conten ...1979501453
improvement in hc1-extractability of minerals in home made weaning foods.three weaning foods were formulated from locally available cereals and pulses such as rice (oryza sativa), kangini (setaria italica), sanwak (echinochloa frumentacea), green gram (vigna radiata) and jaggery. cereals and pulses were mixed in the proportion of 7:3. nutrient composition of developed weaning foods was within range prescribed by indian standard institute and was found to be acceptable. roasting was the processing technique employed in developing weaning foods which resulted in signif ...19957792266
the mammalian homologue of mago nashi encodes a serum-inducible protein.the products of at least 11 maternal effect genes have been shown to be essential for proper germ plasm assembly in drosophila melanogaster embryos. here we report the isolation and characterization of the mammalian counterpart for one of these genes (named magoh for mago nashi homologue). the predicted amino acid sequence of mouse and human magoh are completely identical; magoh homologues from the nematode caenorhabditis elegans and rice grain oryza sativa also show a remarkable degree of amino ...19989479507
the human archain gene, arcn1, has highly conserved homologs in rice and drosophila.a novel human gene, arcn1, has been identified in chromosome band 11q23.3. it maps approximately 50 kb telomeric to mll, a gene that is disrupted in a number of leukemia-associated translocation chromosomes. cdna clones representing arcn1 hybridize to 4-kb mrna species present in all tissues tested. sequencing of cdnas suggests that at least two forms of mrna with alternative 5' ends are present within the cell. the mrna with the longest open reading frame gives rise to a protein of 57 kda. alth ...19957782067
rice cationic peroxidase accumulates in xylem vessels during incompatible interactions with xanthomonas oryzae pv oryzae.a cationic peroxidase, po-c1 (molecular mass 42 kd, isoelectric point 8.6), which is induced in incompatible interactions between the vascular pathogen xanthomonas oryzae pv oryzae and rice (oryza sativa l.), was purified. amino acid sequences from chemically cleaved fragments of po-c1 exhibited a high percentage of identity with deduced sequences of peroxidases from rice, barley, and wheat. polyclonal antibodies were raised to an 11-amino acid oligopeptide (poc1a) that was derived from a domain ...19957770527
the genomic organization of non-ltr retrotransposons (lines) from three beta species and five other angiosperms.we have isolated and characterized conserved regions of the reverse transcriptase gene from non-ltr retrotransposons, also called long interspersed nuclear elements (lines), from beta vulgaris, b. lomatogona and b. nana. the novel elements show strong homology to other non-ltr retrotransposons from plants, man and animals. lines are present in all species of the genus beta tested, but there was variation in copy number. analysis by southern hybridization and fluorescent in situ hybridization rev ...19989520275
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