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production of 9-beta-d-arabinofuranosyladenine by a new species of streptomyces and its herbicidal activity.9-beta-d-arabinofuranosyladenine (ara-a) was produced by a new species of streptomyces designated as s. herbaceus. ara-a was found to have potent herbicidal activity against echinochloa crus-galli, digitaria adscendens and chenopodium ficifolium by the treatment with ara-a before germination of these plants. however, oryza sativa had strong resistance to ara-a.1979528365
the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.).the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens.19751170015
molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.).two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec 2.7.4.3.) were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ...19921302636
characterization of a plant sine, p-sine1, in rice genomes.we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ...19921326298
volatile components of unprocessed rice (oryza sativa l.). 19761002944
recognition efficiency of dicotyledoneae-specific promoter and rna processing signals in rice.heterologous gene expression experiments have shown that genes of monocotyledoneae are often not transcribed in dicotyledoneae, or produce pre-mrna that is inefficiently or aberrantly processed. it is however not known how correctly and efficiently dicotyledon-specific gene expression signals are recognized in cells of monocotyledoneae. here we address this question using tobacco (nicotiana tabacum) and rice (oryza sativa) protoplasts transformed with the same hybrid gene constructs. constructs ...19902177137
fingerprinting genomes using pcr with arbitrary primers.simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (pcr). no prior sequence information is required. the method, arbitrarily primed pcr (ap-pcr), involves two cycles of low stringency amplification followed by pcr at higher stringency. we show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. the generality of the method is demonstrated by application to twenty four ...19902259619
food-based solutions are a viable alternative to glucose-electrolyte solutions for oral hydration in acute diarrhoea--studies in a rat model of secretory diarrhoea.a survey of acute diarrhoea and its treatment, in 3 groups of villages in south india, revealed that use of the world health organization oral rehydration solution (who-ors) was poor or virtually non-existent and that several liquid foods were given to children during acute diarrhoea. the effects of the most commonly used, boiled and cooled supernatants of these liquid foods [rice (oryza sativa)-water, ragi (eleusine coracana)-water, arrowroot (maranta arundinacea)-water], and tender coconut-wat ...19902345922
hydrogen isotope effects in the cyclization of d-glucose 6-phosphate by myo-inositol-1-phosphate synthase.myo-inositol-1-phosphate synthase (ec 5.5.1.4) from rat testis, acer pseudoplatanus l. cell culture and oryza sativa l. cell culture, converted d-[5-3h]glucose 6-phosphate to myo-[2-3h]inositol 1-phosphate at rates ranging from 0.21 to 0.48 that of unlabeled substrate. d-[3-3h]- and d-[4-3h]glucose 6-phosphate were converted at approximately the same rate as that of unlabeled substrate. in the case of testis enzyme, storage as a frozen solution further lowered the rate with d-[5-3h]glucose 6-pho ...1977903359
purification and characterization of a trypsin inhibitor from rice bran.a trypsin inhibitor was isolated and purified from the bran of rice, oryza sativa, by extraction with 1% sodium chloride, heat treatment, ammonium sulfate precipitation, ion-exchange chromatography on a cm-sephadex c-25 and gel filtration on a sephadex g-75. the final preparation was homogeneous by electrophoretic analysis. rice bran trypsin inhibitor (rbti) had a molecular weight of about 14,500 and an isoelectric point of 8.07. the amino acids, acid composition was characterized by high conten ...1979501453
nucleotide sequence of citrus limon 26s rrna gene and secondary structure model of its rna.the complete nucleotide sequence of citrus limon 26s rdna has been determined. the sequence has been aligned with large ribosomal rna (l-rrna) sequences of escherichia coli, saccharomyces cerevisiae and oryza sativa. nine extensive expansion segments in dicot 26s rrna relative to e. coli 23s rrna have been identified and compared with analogous segments of monocot, yeast, amphibian and human l-rrnas. a secondary structure model for lemon 26s rrna has been derived based on the refined model of e. ...19902101688
isolation and characterization of a rice gene encoding a basic chitinase.chitinase, which catalyzes the hydrolysis of the beta-1,4-n-acetyl-d-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. a basic chitinase genomic sequence was isolated from a rice (oryza sativa l.) genomic library using a bean chitinase gene fragment as a probe. the complete nucleotide sequence of the rice chitinase rch10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acid ...19912034221
magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses.we have identified genes for pathogenicity toward rice (oryza sativa) and genes for virulence toward specific rice cultivars in the plant pathogenic fungus magnaporthe grisea. a genetic cross was conducted between the weeping lovegrass (eragrostis curvula) pathogen 4091-5-8, a highly fertile, hermaphroditic laboratory strain, and the rice pathogen o-135, a poorly fertile, female-sterile field isolate that infects weeping lovegrass as well as rice. a six-generation backcrossing scheme was then un ...19912016048
leaf surface fungi of oryza sativa linn. 19715105623
effect of water washing, lime treatment and lime and calcium carbonate supplementation on the nutritive value of paddy (oryza sativa) straw. 19695818438
cloning and characterization of repetitive dna sequences from genomes of oryza minuta and oryza australiensis.the value of genome-specific repetitive dna sequences for use as molecular markers in studying genome differentiation was investigated. five repetitive dna sequences from wild species of rice were cloned. four of the clones, pom1, pom4, poma536, and pompb10, were isolated from oryza minuta accession 101141 (bbcc genomes), and one clone, poa237, was isolated from oryza australiensis accession 100882 (ee genome). southern blot hybridization to different rice genomes showed strong hybridization of ...19911955157
active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases.with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ...19826765191
a homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex i is encoded in chloroplast genomes.the chloroplast genomes of marchantia polymorpha, nicotiana tabacum, and oryza sativa contain open reading frames (orfs or potential genes) encoding homologues of some of the subunits of mitochondrial nadh:ubiquinone oxidoreductase (complex i). seven of these subunits (nd1-nd4, nd4l, nd5, and nd6) are products of the mitochondrial genome, and two others (the 49- and 30-kda components of the iron-sulfur protein fraction) are nuclear gene products. these findings have been taken to indicate the pr ...19911901022
a comparative study of cadmium, lead, zinc, ph, and bulk density from the enyigba lead and zinc mine in two different seasons.soil ph and bulk density, as well as the concentrations of cadmium (cd), lead (pb), and zinc (zn) in soils and these metal levels in the leaves of cassava (manihot esculenta) and rice (oryza sativa), both collected, respectively, in the dry season (december) of 1991 and the rainy season (september) of 1992 from the enyigba lead and zinc mine, nigeria, were investigated. interspecific variations in trace element levels in the plant leaves were detected and may reflect the predilection of these pl ...19957498063
organ-dependent regulation of a plant promoter isolated from rice by 'promoter-trapping' in tobacco.a vector containing a transcriptionally inactive neomycin phosphotransferase ii gene was used to select promoter sequences from a pool of random genomic dna fragments. this paper describes how one such sequence (p4.7) isolated from oryza sativa acts as a hormonally regulated promoter in nicotiana tabacum. relative expression ratios in leaf, root, midrib, callus, and stem tissue of tobacco plants are 1:5:4:10:17. histochemical assays show that p4.7 activates the uida reporter gene throughout the ...19911844878
dietary exposure to aflatoxin in benin city, nigeria: a possible public health concern.a total of 100 samples of various foods comprising of 10 samples each of garri (manihot utilis pohl), beans (phaseolus lunatus), yam flour (dioscorea rotundata), cassava flour (manihot esculentum), melon (citrulus lunatus), onion (allium cepa), rice (oryza sativa), plantain (musa paradisiaca), red pepper (capsicum annuum l., solanaceae) and eggs were screened for the presence of aflatoxins. fifty percent of yam flour; 40% of cassava flour; 30% of garri; 20% of beans and melon and 10% of rice yie ...19911777386
identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide.chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ...19921740436
rice scutellum induces agrobacterium tumefaciens vir genes and t-strand generation.for successful transformation of a plant by agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce agrobacterium tumour-inducing (ti) plasmid virulence (vir) genes. here we report a significant variation in different tissues of indica rice (oryza sativa l. cv. co43) in their ability to induce agrobacterium tumefaciens vir genes and t-strand generation, using explants preincubated in liquid murashige and skoog (ms) medium. an analysis of rice le ...19957579158
a novel ras-related rgp1 gene encoding a gtp-binding protein has reduced expression in 5-azacytidine-induced dwarf rice.exposure of normal, tall rice (oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of dna methylation in vivo, induced both demethylation of genomic dna and dwarf plants. genes that had been affected by treatment were identified by differential screening of a cdna library, and a ras-related gene, rgp1, was subsequently isolated. the cdna of rgp1 was found to encode a deduced protein sequence of 226 amino acids with a relative molecular mass of 24850, which was most closely related to ...19911715972
transgenic rice cell lines and plants: expression of transferred chimeric genes.cell suspension-derived rice (oryza sativa l.) protoplasts were transformed by direct gene uptake. peg-mediated transformation was more efficient than electroporation. plasmid dna containing a hygromycin phosphotransferase (hpt) gene (which confers hygromycin resistance) driven by the camv 35s promoter and a beta-d-glucuronidase (gus) gene under control of the 1', 2' double promoter of the mannopine synthase (mas) locus of agrobacterium tumefaciens was introduced into rice protoplasts. southern ...19911713479
the isolation and characterisation of plant sequences homologous to human hypervariable minisatellites.we have isolated dna probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (oryza sativa). these probes are capable of producing a multilocus rice dna fingerprint. the rice sequence has a tandem repeating structure based on a 12 bp gc-rich repeat which shows homology to its human counterpart. this probe detects up to 30 loci which are at a number of unlinked chromosomal sites. the gc-rich sequence is invariably associated with an open reading frame ( ...19911651259
co-transcription of orf25 and coxiii in rice mitochondria.southern hybridization analysis using homologous maize probes indicated that orf25 and coxiii are closely linked in the mitochondrial genome of rice (oryza sativa) cultivar ir36. the two coding regions were found on the same 5.1 kb bamhi fragment, and this fragment was cloned, mapped and partially sequenced. using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mrna transcript was found containing both orf25 and coxiii coding regions. multiple 5' ends were identified by pr ...19921617739
factors influencing agrobacterium-mediated transient expression of gusa in rice.transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ...19921463839
the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase.a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ...19921421143
the atx1 gene of saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity.in aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as superoxide dismutase (sod) and catalase. here we describe the isolation and characterization of another gene in the yeast saccharomyces cerevisiae that plays a critical role in detoxification of reactive oxygen species. this gene, named atx1, was originally isolated by its ability to suppress oxygen toxicity in yeast lacking sod. atx1 encodes a 8.2-kda polypep ...19957731983
efficiency of the 5'-terminal sequence (omega) of tobacco mosaic virus rna for the initiation of eukaryotic gene translation in escherichia coli.recent studies have demonstrated that the 5' leader (omega sequence) of tobacco mosaic virus rna has a certain enhancing capacity for translation of mrna in both prokaryotes and eukaryotes. in order to estimate the efficiency of omega to initiate translation of mrna in escherichia coli, in comparison to the shine-dalgarno (s/d) sequence, we have inserted eight different eukaryotic genes into two types of e. coli expression vectors containing one constitutive promoter (p1) but different translati ...19921396694
isolation and characterization of cdna clones encoding cdc2 homologues from oryza sativa: a functional homologue and cognate variants.using probes obtained by pcr amplification, we have isolated two cognate rice cdnas (cdc2os-1 and cdc2os-2) encoding structural homologues of the cdc2+/cdc28 (cdc2) protein kinase from a cdna library prepared from cultured rice cells. comparison of the deduced amino acid sequences of cdc2os-1 and cdc2os-2 showed that they are 83% identical. they are 62% identical to cdc28 of saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice r2, a cdc2-related ...19921376401
purification and characterization of rice peroxidases.four peroxidase components, named rp-2, 4, 6, and 7, were isolated from rice (oryza sativa l.) green leaves. isoelectric focusing indicated that each preparation was homogeneous. the molecular weights of rp-2, 4, 6, and 7 estimated by sds-page were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. the activity of every preparation was maximum around ph 5.0. antisera against these purified enzymes were raised in rabbits. ouchterlony double ...19911368754
thioredoxin h is one of the major proteins in rice phloem sap.sieve tubes play important roles in the transfer of nutrients as well as signals. hundreds of proteins were found in pure phloem sap collected from rice (oryza sativa l. cv. kantou) plants through the cut ends of insect stylets. these proteins may be involved in nutrient transfer and signal transduction. to characterize the nature of these proteins, the partial amino-acid sequence of a 13-kda protein, named rpp13-1, that was abundant in the pure phleom sap was determined. a cdna clone of 687 bp, ...19957766047
genetic and physical analysis of the rice bacterial blight disease resistance locus, xa21.nearly isogenic lines (nils) of rice (oryza sativa) differing at a locus conferring resistance to the pathogen xanthomonas oryzae pv. oryzae were surveyed with 123 dna markers and 985 random primers using restriction fragment length plymorphism (rflp) and random amplified polymorphic dna (rapd) analysis. one chromosome 11 marker (rg103) detected polymorphism between the nils that cosegregated with xa21. all other chromosome 11 dna markers tested were monomorphic between the nils, localizing the ...19921362973
[larval density of anopheles (n) albimanus wiedemann, 1821 in relation to phenology of oryza sativa (linnaeus) rice].the larval density of anopheles (n) albimanus wiedemann, 1821, is linked with rice phenology in the two crops of the year (summer and winter). it was determined that a higher density agrees with the second stage of the vegetative cycle of the culture named beginning of shooting processes and the formation of the panicular primordium. the number of anopheline larvae was higher in the summer.19892672175
the complete sequence of the rice (oryza sativa) chloroplast genome: intermolecular recombination between distinct trna genes accounts for a major plastid dna inversion during the evolution of the cereals.the entire chloroplast genome of the monocot rice (oryza sativa) has been sequenced and comprises 134525 bp. predicted genes have been identified along with open reading frames (orfs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (nicotiana tabacum), and a liverwort (marchantia polymorpha). the same complement of 30 trna and 4 rrna genes has been conserved between rice and tobacco. most orfs extensively conserved between n. tabacum and m. polymorpha ar ...19892770692
mutagenic effect of argemone oil in oryza sativa l.in view of the large scale adulteration of edible oils with argemone oil, a study was undertaken to test its mutagenicity on rice seed. in treatment with screw-pressed oil a marked reduction in seed germination and high chlorophyll mutation and mutant frequencies were observed. in sequential treatments between gamma rays and argemone oil both mutation and mutant frequencies were significantly higher than that of individual treatments. this study clearly indicates that argemone oil is a potent mu ...1977876267
[bean and rice mixtures of high protein value (author's transl)].the optimum content of different rice (oryza sativa) and bean (vigna unguiculata l.) combinations was studied in albino rats. the mixture containing 3 g of rice proteins, corresponding to 41g of rice "in natura", and 7 g of bean protein, corresponding to 59 g of bean "in natura", presented the highest protein efficiency ratio and the feed efficiency ratio. since the limiting amino acid of this mixture was methionine, new assays using varying levels of this amino acid as a supplement were carried ...1979515465
herbimycin, a new antibiotic produced by a strain of streptomyces.herbimycin, a new antibiotic, was isolated from the fermentation broth of streptomyces hygroscopicus strain no. am-3672, a soil isolate. the molecular formula of herbimycin was determined to be c30h42n2o9. herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against cyperus microiria steud. however, oryza sativa showed strong resistance to herbimycin.1979468713
protein quality in cereals and pulses. 1. application of microbiological and other in vitro methods in the evaluation of rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.).1. the streptococcus zymogenes assay procedure was modified and used in the estimation of available methionine and relative nutritional value in rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). the results were assessed in relation to the content of tannins and 'dye-binding lysine', and to published information on the nutritional quality of some of the test samples. 2. in grain of ten varieties of sorghum, for which other workers had reported a cor ...1979427087
the effects of fruit juices and fruits on the absorption of iron from a rice meal.the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...19873593665
isolation, molecular and biological properties of a lectin from rice embryo: relationship with wheat germ agglutinin properties.rice lectin (oryza sativa, var. balilla 28) was purified from defatted embryos by aqueous acid extraction at ph 1.3 followed by ammonium sulfate precipitation between 2 and 4 m, affinity chromatography on agarose-p-aminophenyl-beta-d-n-acetylglucosamine, and gel filtration on aca 54. the homogeneity of the lectin was checked by polyacrylamide gel electrophoresis, gel filtration, and immunodiffusion. the amino acid analysis revealed a high half-cystine content (9%) and a low aromatic and hydropho ...19873688888
[evaluation of nutritional potential of fish in diets based on beans (phaseolus vulgaris) and a cereal/corn (zea mays) and/or rice (oryza sativa)].p6e complementation between corn and fish, and rice and fish was evaluated for the purpose of establishing the required levels of each constituent in the mixture to obtain the optimum value in the biological evaluations for each diet. the optimum fish levels were around 10 and 6% for the corn and rice diets, respectively. complementary levels of fish in the bean: corn and bean:rice diets were evaluated in the same manner. the best values in the biological evaluation were obtained with only 2% of ...19836675550
nramp defines a family of membrane proteins.nramp (natural resistance-associated macrophage protein) is a newly identified family of integral membrane proteins whose biochemical function is unknown. we report on the identification of nramp homologs from the fly drosophila melanogaster, the plant oryza sativa, and the yeast saccharomyces cerevisiae. optimal alignment of protein sequences required insertion of very few gaps and revealed remarkable sequence identity of 28% (yeast), 40% (plant), and 55% (fly) with the mammalian proteins (46%, ...19957479731
a novel lipoxygenase from rice. primary structure and specific expression upon incompatible infection with rice blast fungus.a novel lipoxygenase cdna (3,007 base pairs) was isolated from rice leaves (oryza sativa cv. aichiasahi) which had been infected with an incompatible race of the rice blast fungus, magnaporthe grisea. a single copy of the gene is present in the rice genome and encodes a protein of 923 residues with a molecular weight of 102,714. this gene product shares the least amino acid sequence homology among plant lipoxygenases identified to date. a novel feature of this gene product is a putative transit ...19947508918
palindromic repeated sequences (prss) in the mitochondrial genome of rice: evidence for their insertion after divergence of the genus oryza from the other gramineae.we have identified a family of small repeated sequences (from 60 to 66 bp in length) in the mitochondrial genome of rice (oryza sativa cv. nipponbare). there are at least ten copies of these sequences and they are distributed throughout the mitochondrial genome. each is potentially capable of forming a stem-and-loop structure and we have designated them prss (palindromic repeated sequences). their features are reminiscent of the small dispersed repeats in the mitochondrial dna (mtdna) of some lo ...19947509206
identification, cdna cloning, and gene expression of soluble starch synthase in rice (oryza sativa l.) immature seeds.three forms of soluble starch synthase were resolved by anion-exchange chromatography of soluble extracts from immature rice (oryza sativa l.) seeds, and each of these forms was further purified by affinity chromatograph. the 55-, 57-, and 57-kd proteins in the three preparations were identified as candidates for soluble starch synthase by western blot analysis using an antiserum against rice granule-bound starch synthase. it is interesting that the amino-terminal amino acid sequence was identic ...19937518089
an amino-terminal deletion of rice phytochrome a results in a dominant negative suppression of tobacco phytochrome a activity in transgenic tobacco seedlings.overexpression of phytochrome a results in an increased inhibition of hypocotyl elongation under red and far-red light. we used this approach to assay for the function of n-terminal mutations of rice (oryza sativa l.) phytochrome a. transgenic tobacco seedlings that express the wild-type rice phytochrome a (rw), a rice phytochrome a lacking the first 80 amino acids (ntd) or a rice phytochrome a with a conversion of the first 10 serines into alanine residues (s/a) were compared with untransformed ...19957580859
2,3-bisphosphoglycerate-independent phosphoglycerate mutase is conserved among different phylogenic kingdoms.we have previously demonstrated that maize (zea mays) 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgam-i) is not related to 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase. with the aid of specific anti-maize pgam-i antibodies, we demonstrate here the presence of a closely related pgam-i in other plants. we also describe the isolation and sequencing of a cdna-encoding almond (prunus amygdalus) pgam-i that further demonstrates this relationship among plant pgam-i. a sea ...19957584858
the regulatory regions of the rice tungro bacilliform virus promoter and interacting nuclear factors in rice (oryza sativa l.).the rice tungro bacilliform virus (rtbv) promoter confers phloem-specific gene expression in transgenic rice plants. a series of promoter deletion mutants were fused with the escherichia coli beta-glucuronidase a (uida) reporter gene and introduced into transgenic rice plants. the rtbv promoter confers substantially stronger expression in shoots than in roots. a fragment of the promoter comprising nucleotides -164 to +45 relative to the transcriptional start site contains sufficient information ...19957599653
fractionation and characterization of two forms of peroxidase from oryza sativa.peroxidase (e.c. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ...19957603969
a view of plant dehydrins using antibodies specific to the carboxy terminal peptide.dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ...19937693020
root colonization and systemic spreading of azoarcus sp. strain bh72 in grasses.the invasive properties of azoarcus sp. strain bh72, an endorhizospheric isolate of kallar grass, on gnotobiotically grown seedlings of oryza sativa ir36 and leptochloa fusca (l.) kunth were studied. additionally, azoarcus spp. were localized in roots of field-grown kallar grass. to facilitate localization and to assure identity of bacteria, genetically engineered microorganisms expressing beta-glucuronidase were also used as inocula. beta-glucuronidase staining indicated that the apical region ...19948144457
proline accumulates in plants exposed to uv radiation and protects them against uv induced peroxidation.proline accumulated in the shoots of seedlings of rice (oryza sativa), mustard (brassica juncea) and mung bean (vigna radiata) exposed to uv radiations. the level of proline in the seedlings increased significantly with increase in uv exposure time. the production of malondialdehyde (an indice of lipid peroxidation) was also higher in the shoots of seedlings exposed to uv radiation as compared to controls, suggesting that uv radiations promote lipid peroxidation. the extent of uv radiation promo ...19957726821
metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.fluorescence in situ hybridization (fish) is a powerful tool for physical mapping in human and other mammalian species. however, application of the fish technique has been limited in plant species, especially for mapping single- or low-copy dna sequences, due to inconsistent signal production in plant chromosome preparations. here we demonstrate that bacterial artificial chromosome (bac) clones can be mapped readily on rice (oryza sativa l.) chromosomes by fish. repetitive dna sequences in bac c ...19957753830
six new candidate members of the alpha/beta twisted open-sheet family detected by sequence similarity to flavodoxin.strong sequence similarity has been reported among wrba (the trp repressor-binding protein of escherichia coli); ycp4, a protein of unknown function from the budding yeast saccharomyces cerevisiae; p25, the pap1-dependent protein of the fission yeast schizosaccharomyces pombe; and the translation product of a partial cdna sequence from rice seedling root (oryza sativa, locus ricr02421a; here referred to as ricr). further homology search with the profile method indicates that all the above sequen ...19947756978
heat-inducible rice hsp82 and hsp70 are not always co-regulated.we have characterized several heat-shock-induced genes in rice (oryza sativa l.) and compared their expression under a variety of conditions. three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. the middle member of this cluster, designated hsp82b, has been fully sequenced. the gene uses a promoter containing six putative heat-shock elements as well as several unusual sequence motifs including a stretch of 11 thymidines altern ...19947764623
expression of functional proteins of cdna encoding rice nucleoside diphosphate kinase (ndk) in escherichia coli and organ-related alteration of ndk activities during rice seed germination (oryza sativa l.).the gst (glutathione s-transferase)-ndk (nucleoside diphosphate kinase) fusion protein was expressed in escherichia coli. the gst-ndk protein was capable of transferring gamma-phosphate from atp to nucleoside diphosphates such as gdp, cdp, tdp and udp. western blot analysis using anti-ndk antibody indicated that ndk in endosperm gradually decreased during 36 h of imbibition. on the contrary, ndk in embryo increased during the same period. ndk activities in both tissues were in accord with these ...19957766875
biochemical properties of rice adenylate kinase and subcellular location in plant cells.previously, we characterized nucleotide sequences of two cdnas encoding adenylate kinase from rice plants (oryza sativa l.). each cdna (adk-a or adk-b) was cloned into the expression vector pet 11d-gst to produce gst-ak fusion proteins in escherichia coli. recombinant proteins were cleaved by thrombin, and gst-free adenylate kinase proteins were obtained. enzyme activity profiles of different ph and inhibition effects to the enzyme by ap5a (adenosine-5'-pentaphospho-5'-adenosine) indicates that ...19957766884
molecular cloning and characterization of rga1 encoding a g protein alpha subunit from rice (oryza sativa l. ir-36).a cdna clone, rga1, was isolated by using a gpa1 cdna clone of arabidopsis thaliana g protein alpha subunit as a probe from a rice (oryza sativa l. ir-36) seedling cdna library from roots and leaves. sequence analysis of genomic clone reveals that the rga1 gene has 14 exons and 13 introns, and encodes a polypeptide of 380 amino acid residues with a calculated molecular weight of 44.5 kda. the encoded protein exhibits a considerable degree of amino acid sequence similarity to all the other known ...19957766894
rice cationic peroxidase accumulates in xylem vessels during incompatible interactions with xanthomonas oryzae pv oryzae.a cationic peroxidase, po-c1 (molecular mass 42 kd, isoelectric point 8.6), which is induced in incompatible interactions between the vascular pathogen xanthomonas oryzae pv oryzae and rice (oryza sativa l.), was purified. amino acid sequences from chemically cleaved fragments of po-c1 exhibited a high percentage of identity with deduced sequences of peroxidases from rice, barley, and wheat. polyclonal antibodies were raised to an 11-amino acid oligopeptide (poc1a) that was derived from a domain ...19957770527
the human archain gene, arcn1, has highly conserved homologs in rice and drosophila.a novel human gene, arcn1, has been identified in chromosome band 11q23.3. it maps approximately 50 kb telomeric to mll, a gene that is disrupted in a number of leukemia-associated translocation chromosomes. cdna clones representing arcn1 hybridize to 4-kb mrna species present in all tissues tested. sequencing of cdnas suggests that at least two forms of mrna with alternative 5' ends are present within the cell. the mrna with the longest open reading frame gives rise to a protein of 57 kda. alth ...19957782067
chromosomal locations of ten isozyme loci in rice (oryza sativa l.) through trisomic analysis.chromosomal locations of 10 isozyme loci in rice (oryza sativa l.) were determined through trisomic analysis. all 10 genes produced altered allozyme banding patterns in specific f1 trisomics. this served as the primary source of evidence for chromosome locations of est-5, icd-1, acp-1, and pgd-1. the locations of amp-1, amp-2, amp-4, pox-5, got-1, and cat-1 were further confirmed from segregation data in bc1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but ...19883408480
open reading frame 176 in the photosynthesis gene cluster of rhodobacter capsulatus encodes idi, a gene for isopentenyl diphosphate isomerase.isopentenyl diphosphate (ipp) isomerase catalyzes an essential activation step in the isoprenoid biosynthetic pathway. a database search based on probes from the highly conserved regions in three eukaryotic ipp isomerases revealed substantial similarity with orf176 in the photosynthesis gene cluster in rhodobacter capsulatus. the open reading frame was cloned into an escherichia coli expression vector. the encoded 20-kda protein, which was purified in two steps by ion exchange and hydrophobic in ...19968550491
protein quality of weaning foods based on locally available cereal and pulse combination.locally available cereals and pulses such as rice (oryza sativa), kangini (setaria italica), sanwak (echinochloa frumentacea), green gram (vigna radiata) and jaggery were used to formulate three weaning foods. cereal, pulse and jaggery were mixed in the ratio of 70:30:25. roasting was the main processing technique used in the formulation of these weaning foods. the developed weaning foods had 5.06 to 5.68 g moisture, 10.28 to 13.71 g protein, 2.91 to 3.77 g ash, 1.08 to 1.87 g fat, 14.42 to 14.9 ...19947855096
inheritance of gusa and neo genes in transgenic rice.inheritance of foreign genes neo and gusa in rice (oryza sativa l. cv. ir54 and radon) has been investigated in three different primary (t0) transformants and their progeny plants. t0 plants were obtained by co-transforming protoplasts from two different rice suspension cultures with the neomycin phosphotransferase ii gene [neo or aph (3') ii] and the beta-glucuronidase gene (uida or gusa) residing on separate chimeric plasmid constructs. the suspension cultures were derived from callus of immat ...19957865799
molecular cloning of the full-length cdna of (s)-hydroxynitrile lyase from hevea brasiliensis. functional expression in escherichia coli and saccharomyces cerevisiae and identification of an active site residue.the full-length cdna of (s)-hydroxynitrile lyase (hnl) from leaves of hevea brasiliensis (tropical rubber tree) was cloned by an immunoscreening and sequenced. hnl from h. brasiliensis is involved in the biodegradation of cyanogenic glycosides and also catalyzes the stereospecific synthesis of aliphatic, aromatic, and heterocyclic cyanohydrins, which are important as precursors for pharmaceutical compounds. the open reading frame identified in a 1. 1-kilobase cdna fragment codes for a protein of ...19968621461
novel drosophila melanogaster genes encoding rrm-type rna-binding proteins identified by a degenerate pcr strategy.we are interested in identifying drosophila melanogaster rna-binding proteins involved in important developmental decisions made at the level of mrna processing, stability, localization or translational control. a large subset of the proteins known to interact with specific rna sequences shares an evolutionarily conserved 80-90-amino-acid (aa) domain referred to as an rna-recognition motif (rrm), including two ribonucleoprotein identifier sequences known as rnp-1 and rnp-2. hence, we have herein ...19957890163
expression of functional proliferating-cell nuclear antigen from rice (oryza sativa) in escherichia coli. activity in association with human dna polymerase delta.proliferating-cell nuclear antigen (pcna), the auxiliary protein for dna polymerase delta, is one of the key factors for both pcna-dependent dna synthesis and cell-cycle progression. plant pcna genes have previously been cloned from rice, carrot, tobacco, and soybean cells by screening the cdna libraries using similarity to the human or rat pcna genes. we subcloned the relevant gene from the rice pcna cdna into an escherichia coli expression vector pmal, and the pcna protein was expressed in the ...19947913441
efficient transformation of rice (oryza sativa l.) mediated by agrobacterium and sequence analysis of the boundaries of the t-dna.a large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with agrobacterium tumefaciens. the efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the r0, r1 and r2 generations. sequence analysis revealed that the ...19947920717
on the identification of group ii introns in nucleotide sequence data.four different consensus sequences (gti, group ii identifiers) have been derived from domains v of known group ii introns and are used as query input sequences for sensitive database screenings with the fasta and lfasta programs. the set of four gti sequences can identify all domains v of the 96 known group ii introns in the completely sequenced chloroplast genomes of marchantia polymorpha, epifagus virginiana, oryza sativa, nicotiana tabacum and the completely sequenced mitochondrial genomes of ...19947932698
a likelihood approach for comparing synonymous and nonsynonymous nucleotide substitution rates, with application to the chloroplast genome.a model of dna sequence evolution applicable to coding regions is presented. this represents the first evolutionary model that accounts for dependencies among nucleotides within a codon. the model uses the codon, as opposed to the nucleotide, as the unit of evolution, and is parameterized in terms of synonymous and nonsynonymous nucleotide substitution rates. one of the model's advantages over those used in methods for estimating synonymous and nonsynonymous substitution rates is that it complet ...19947968485
molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice.a receptor-like protein kinase, ospk10, has been cloned from rice (oryza sativa). the 2.8 kb cdna contains an open reading frame capable of encoding a peptide sequence of 824 amino acids. the topological features of the predicted ospk10 protein include an n-terminal signal peptide, a cysteine-rich extracellular ligand-binding domain, a membrane-spanning segment, and a cytoplasmic domain possessing all the hallmarks of catalytic domains of eukaryotic protein kinases. the cytoplasmic domain was se ...19947999995
isolation of a monocot 3-hydroxy-3-methylglutaryl coenzyme a reductase gene that is elicitor-inducible.the rice (oryza sativa) phytoalexins, momilactones and oryzalexins, are synthesized by the isoprenoid pathway. an early step in this pathway, one that is rate-limiting in mammalian systems, is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr). a gene that encodes this enzyme has been isolated from rice, and found to contain an open reading frame of 1527 bases. the encoded protein sequence of the rice hmgr appears to be conserved with respect to other hmgr proteins, a ...19948049366
cloning and structural analysis of calmodulin gene from rice.full-length cdna encoding rice (oryza sativa, cv. nongken 58s) calmodulin (cam) mrna has been cloned using polymerase chain reaction (pcr) with synthesized primers from the sequence of barley cam gene. sequence analysis revealed that the rice gene is 450 bp in length that and its predicted polypeptide consists of 148 amino acids. it differs from those that of barley and alfalfa in only one position, respectively. the nucleotide homology between rice and barley is 90%, between rice and alfalfa is ...19938061233
rice tungro bacilliform virus: transcription and translation in protoplasts.protoplasts from cell suspension cultures of oryza sativa (monocot) and orychophragmus violaceus (dicot) support transcription from the rice tungro bacilliform virus (rtbv) promoter and translation of the resulting mrna despite the presence of a long leader sequence with strong secondary structure and 12 short open reading frames. transcriptional elements located both upstream and downstream of the transcription initiation site are defined by deletion analysis and the functional tata motif is de ...19948091688
a rice (oryza sativa l.) cdna encodes a protein sequence homologous to the eukaryotic ribosomal 5s rna-binding protein.a rice (oryza sativa l.) cdna clone coding for the cytoplasmic ribosomal protein l5, which associates with 5 s rrna for ribosome assembly, was cloned and its nucleotide sequence was determined. the primary structure of rice l5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. these include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three ...19938219074
improvement in hc1-extractability of minerals in home made weaning foods.three weaning foods were formulated from locally available cereals and pulses such as rice (oryza sativa), kangini (setaria italica), sanwak (echinochloa frumentacea), green gram (vigna radiata) and jaggery. cereals and pulses were mixed in the proportion of 7:3. nutrient composition of developed weaning foods was within range prescribed by indian standard institute and was found to be acceptable. roasting was the processing technique employed in developing weaning foods which resulted in signif ...19957792266
structure and differential response to abscisic acid of two promoters for the cytosolic copper/zinc-superoxide dismutase genes, sodcc1 and sodcc2, in rice protoplasts.we determined the 5'-flanking sequences of two nuclear genes (sodcc1 and sodcc2) encoding cytosolic copper/zinc-superoxide dismutase in rice (oryza sativa l.). utilizing transient beta-glucuronidase (gus) reporter assays, functional promoter-gus analysis was performed in rice protoplasts exposed to the phytohormone abscisic acid (aba) or the antioxidant sulfhydryl reagent, dithiothreitol (dtt). transcriptional activities from both sodcc-gus fusions were stimulated by dtt, which induces the promo ...19957821431
synthesis of an infectious full-length cdna clone of rice yellow mottle virus and mutagenesis of the coat protein.a full-length cdna clone of rice yellow mottle sobemovirus (rymv) was synthesized and placed adjacent to a bacteriophage t7 rna polymerase promoter sequence. capped-rna transcripts produced in vitro were infectious when mechanically inoculated onto rice plants (oryza sativa l). individual full-length clones varied in their degree of infectivity but all were less infectious than native viral rna. a representative clone, designated rymv-fl5, caused a disease phenotype identical to that produced by ...19957831766
chloroplast dna variability in wild and cultivated rice (oryza spp.) revealed by polymorphic chloroplast simple sequence repeats.short mononucleotide repeats analogous to nuclear microsatellites or simple sequence repeats (ssrs) have been identified in chloroplast genomes. primers flanking mononucleotide repeats in the fully sequenced rice chloroplast genome have been used in conjunction with pcr to amplify genomic dna from 42 wild rice accessions. the amplification products exhibited length polymorphism, which allowed the levels of chloroplast variability detected to be quantified. seven primer pairs that amplified produ ...19979061917
cloning and characterization of the rice cata catalase gene, a homologue of the maize cat3 gene.we isolated and sequenced a genomic clone (cata) encoding cat-a catalase, a homologue of the maize catalase isozyme 3 (cat-3) from rice (oryza sativa l.). the 5'-upstream non-coding region had very low similarity with the maize cat3 gene and possible cis elements and sequence motifs in the maize cat3 gene were not evident, except for tata and caat motifs. several sequence motifs found in the promoters of plant seed-specific genes were identified in the 5'-upstream non-coding region of the cata g ...19968605302
a novel cross-phylum family of proteins comprises a krr1 (ycl059c) gene which is essential for viability of saccharomyces cerevisiae cells.we demonstrate here that the open reading frame (orf) ycl059c, discovered during the systematic sequencing of chromosome iii [oliver et al., nature 357 (1992) 38-46], codes for a protein essential for yeast: neither spore germination nor cell division occur in strains deleted for this gene. we have cloned the wild-type (wt) gene and shown that it complements the deletion. a relatively abundant rna transcript corresponds to the gene. the protein has no similarity to proteins of known function. in ...19968675026
starch debranching enzyme (r-enzyme or pullulanase) from developing rice endosperm: purification, cdna and chromosomal localization of the gene.starch debranching enzyme (r-enzyme or pullulanase) was purified to homogeneity from developing endosperm of rice (oryza sativa l. cv. fujihikari) using a variety of high-performance liquid chromatography columns, and characterized. a cdna clone encoding the full length of the rice endosperm debranching enzyme was isolated and its nucleotide sequence was determined. the cdna contains an open reading frame of 2958 bp. the mature debranching enzyme of rice appears to be composed of 912 amino acids ...19968680309
the drosophila melanogaster gene vha14 encoding a 14-kda f-subunit of the vacuolar atpase.a drosophila melanogaster (dm) cdna (vha14) encoding the 14-kda f-subunit of the vacuolar h(+)-atpase (v-atpase) has been cloned via homology with the corresponding manduca sexta (ms) gene. its deduced translation product is a 124-amino-acid polypeptide sharing 90% identity with the ms polypeptide and 50% identity with an analogous polypeptide of saccharomyces cerevisiae, and a more distant similarity to a subunit of the na(+)-transporting atpase of enterococcus hirae. homology was also found wi ...19968682310
characterization of cdna encoding for phosphoglucose isomerase of rice (oryza sativa l.).two types of genes (pgi-a and pgi-b) encoding phosphoglucose isomerase (pgi; ec 5.3.1.9) were cloned from cdna libraries of rice cultured cells (oryza sativa l.). pgi-a and pgi-b consisted of 2132 and 2030 nucleotides, respectively. the homology between these genes was 93.0% at nucleotide level. the homology scores between these genes in protein coding region and 3' non-coding region were 95.6% and 79.4%, respectively. pgi proteins encoded by pgi-a and pgi-b consisted of 567 and 568 amino acid, ...19968722567
vectors carrying two separate t-dnas for co-transformation of higher plants mediated by agrobacterium tumefaciens and segregation of transformants free from selection markers.novel 'super-binary' vectors that carried two separate t-dnas were constructed. one t-dna contained a drug-resistance, selection-marker gene and the other contained a gene for beta-glucuronidase (gus). a large number of tobacco (nicotiana tabacum l.) and rice (oryza sativa l.) transformants were produced by agrobacterium tumefaciens lba4404 that carried the vectors. frequency of co-transformation with the two t-dnas was greater than 47% gus-positive, drug-sensitive progeny were obtained from mor ...19968758986
phosphorylation of a protein (pp56) is related to the regeneration of rice cultured suspension cells.short-term cultured suspension cells of rice (oryza sativa l.) are capable of regeneration, but not in long-term culture. for clarification of the mechanism of regeneration, protein phosphorylation in short-term and long-term cultured suspension cells was compared by two dimensional-polyacrylamide gel electrophoresis. a 56 kda protein having an isoelectric point of 4.5 was phosphorylated in vitro in short-term cultured suspension cells, but was not phosphorylated after regeneration. this protein ...19968888618
higher plants contain homologs of the bacterial cela genes encoding the catalytic subunit of cellulose synthase.in spite of much effort, no one has succeeded in isolating and characterizing the enzyme(s) responsible for synthesis of cellulose, the major cell wall polymer of plants. we have characterized two cotton (gossypium hirsutum) cdna clones and identified one rice (oryza sativa) cdna that are homologs of the bacterial cela genes that encode the catalytic subunit of cellulose synthase. three regions in the deduced amino acid sequences of the plant cela gene products are conserved with respect to the ...19968901635
development of microsatellite markers and characterization of simple sequence length polymorphism (sslp) in rice (oryza sativa l.).microsatellite markers containing simple sequence repeats (ssr) are a valuable tool for genetic analysis. our objective is to augment the existing rflp map of rice with simple sequence length polymorphisms (sslp). in this study, we describe 20 new microsatellite markers that have been assigned to positions along the rice chromosomes, characterized for their allelic diversity in cultivated and wild rice, and tested for amplification in distantly related species. our results indicate that the geno ...19968914521
physicochemical and serological characterization of rice alpha-amylase isoforms and identification of their corresponding genes.we have identified, purified, and characterized 10 alpha-amylase isoforms from suspension-cultured rice (oryza sativa l.) cells having different isoelectric point values. they had distinguishable optimum temperatures for enzymatic activity and molecular sizes. the results of immunoblotting indicated that polyclonal anti-a + b antibodies bound well to isoforms a, b, y, and z but weakly or not at all to e, f, g, h, i, and j. however, the anti-a + b antibodies inhibited the enzyme activities of onl ...19968934629
nascent transcript-binding protein of the pea chloroplast transcriptionally active chromosome.this study describes the nascent rna-binding protein of the pea chloroplast transcriptional complex. the protein has been identified by photoaffinity labelling of the transcriptionally active chromosome (tac) which utilizes the endogenous plastid dna as template. uv irradiation of lysed chloroplast or the isolated tac under conditions optimized for transcription photocross-links nascent radiolabelled transcripts (up to 250 nucleotides in length) to a 48 kda protein. the photoaffinity labelling o ...19938260634
clusters of interspersed repeated dna sequences in the rice genome (oryza).we have characterized a repeated dna sequence (rtl122) from rice (oryza sativa l.) with respect to its organization in the rice genome and its distribution among rice and other plants. the results indicate that the rtl122 sequence is interspersed in the rice genome and limited to the genus oryza. it is highly polymorphic and can be used to fingerprint rice varieties. a structure was observed in which several repeated sequences were clustered in dna regions of 15-20 kb. we characterized three bac ...19938270205
characterization of minisatellite sequences from oryza sativa.two dna sequences were cloned from the genome of cultivated rice (oryza sativa l.) by cross-hybridization with the human minisatellite sequence 33.6. the rice sequences consisted of tandem direct repeats, which showed significant similarity to the 33.6 consensus sequence. profiles capable of distinguishing different rice cultivars were detected by cross-hybridization with a dna probe amplified by the polymerase chain reaction from one of the rice minisatellite sequences.19938270207
water permeability of plasma membranes of cultured rice, grape, and ch27 cells measured dielectrically.the capacitance of suspensions of cultured rice cells (oryza sativa l. ssp. japonica), grape cells (vitis sp.), and ch27 cells originated from murine b-cell lymphoma was measured in the frequency range of 0.2 to 10 mhz. the relationship between the increase in capacitance caused by the presence of cells at 0.4 mhz, delta c, and the cell density was linear. measurement of capacitance was useful in measurement of transitional changes in cell volume under external osmotic stress when sucrose was ad ...19979404061
cloning and sequence analysis of a cdna encoding rice glutaredoxin.a full-length cdna clone (rasc8) encoding glutaredoxin (thioltransferase) was isolated from a cdna library of an aleurone layer prepared from a developing seed of rice (oryza sativa l.). rasc8, 568bp in length, contained an atg codon and two possible polyadenylation signals, and encoded 112 amino acid residues. cys-pro-phe-cys, which is the active site and a highly conserved sequence among thioltransferases, was found in the deduced amino acid sequence. rasc8 was introduced into an expression ve ...19948287970
a 127 kda component of a uv-damaged dna-binding complex, which is defective in some xeroderma pigmentosum group e patients, is homologous to a slime mold protein.a cdna which encodes a approximately 127 kda uv-damaged dna-binding (uv-ddb) protein with high affinity for (6-4)pyrimidine dimers [abramic', m., levine, a.s. & protic', m., j. biol. chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cdna library. the presence of this protein in complexes bound to uv-damaged dna was confirmed by immunoblotting. the human cognate of the uv-ddb gene was localized to chromosome 11. uv-ddb mrna was expressed in all human tissues examined, including c ...19938371985
anaerobiosis and plant growth hormones induce two genes encoding 1-aminocyclopropane-1-carboxylate synthase in rice (oryza sativa l.).the plant hormone ethylene is believed to be responsible for the ability of rice to grow in the deepwater regions of southeast asia. ethylene production is induced by hypoxia, which is caused by flooding, because of enhanced activity of 1-aminocyclopropane-1-carboxylic acid (acc) synthase, the key enzyme in the ethylene biosynthetic pathway. we have cloned three divergent members, (os-acs1, os-acs2, and os-acs3), of a multigene family encoding acc synthase in rice. os-acs1 resides on chromosome ...19938389618
agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene.we have successfully transferred and expressed a reporter gene driven by an alpha-amylase promoter in a japonica type of rice (oryza sativa l. cv. tainung 62) using the agrobacterium-mediated gene transfer system. immature rice embryos (10-12 days after anthesis) were infected with an agrobacterium strain carrying a plasmid containing chimeric genes of beta-glucuronidase (uida) and neomycin phosphotransferase (nptii). co-incubation of potato suspension culture (psc) with the agrobacterium inocul ...19938392395
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