comparison between magic lite- and cap-system: two automated specific ige antibody assays.the magic lite system, a newly developed immunochemiluminometric assay for specific and total ige antibody using paramagnetic particles coupled with standardized allergens as solid phase, was compared to the cap system, a recently introduced immunoassay based on a cellulose polymer encased in a capsule. a total of 357 serum samples of patients with suspected inhalant allergies and a positive skin prick test (spt) to common allergens (birch, timothy-grass, mugwort, cat dander, dermatophagoides pt ...19921611547
[pollinosis and oral allergy syndrome].allergic diseases are frequent, affecting 10%-15% of the population. the atopic symptoms manifest mainly as pollinosis or bronchial asthma. many of the atopic patients have an additional food-related allergy, often due to a cross-reactivity between pollen allergens (birch, hazelnut, alder, mugwort) and food allergens. the foods which most frequently elicit oral, gastro-intestinal or anaphylactic symptoms are fruits such as apples, peaches, cherries or apricots, but also nuts and vegetables from ...19902373647
allergy to artemisia vulgaris in the region of warsaw.from 446 pollinic patients, 398 of them (89%) were sensitive to the pollen of graminae and 187 (42%) to artemisia vulgaris. sensitivity to the antigens of both increased the possibility of bronchial symptoms among pollinosis patients from 38% when only the allergy to graminae was present (up to 51%). seventy eight percent of patients allergic exclusively to the pollen of grasses become symptomatic below the age of 20, whereas among 48 patients sensitive only to artemisia, in 71% the clinical sym ...19902382597
comparison between modified skin prick test with standardized allergen extracts and prick tests (spts) represent one of the most common techniques of skin testing and are of great value, especially when standardized extracts are used. phazet is a puncture test involving needles coated with standardized allergens. phazet and spts have been compared in 130 subjects tested with six standardized allergen extracts: orchard-grass, olive, and mugwort pollens, cat dander, dermatophagoides pteronyssinus, and alternaria. patients have been carefully selected on the basis of a sugges ...19883192871
purification of the glycoprotein allergen ag7 from mugwort pollen by concanavalin a affinity chromatography.two affinity columns comprising immobilized concanavalin a (con a), con a-sepharose and con a-xp3507, were evaluated for their purifying ability for the glycoprotein allergen ag7 from a partially purified extract of mugwort pollen. the most pronounced difference between the two columns was the nature of their nonspecific interactions; hydrophobic interactions were dominant with con a-xp3507, whereas ionic interactions were dominant with con a-sepharose. both con a-columns were effective for puri ...19901366940
celery allergy associated with birch and mugwort prick tests (spt) with various celery, carrot and potato preparations (raw, cooked, cooking water of each vegetable and allergen extracts) as well as specific ige determinations by rast to celery mix, celeriac (or root celery), stick celery and heated celery extracts were performed in 70 patients with positive prick or intracutaneous tests to birch and/or mugwort pollens and celery (extract and/or raw). 94% of the patients showed positive prick tests to raw celeriac, 36% to cooked celeriac ...19902288391
[sensitivity to mugwort pollen allergens (artemisia vulgaris)].the author has found that 42% of patients with pollinosis had positive skin reactions with mugwort (artemisia vulgaris) pollen allergens. the majority of tested patients (139 out of 187) were also allergic to grass pollens. however, hypersensitivity to mugwort pollen allergens was isolated and did not accompany grass pollen allergy. the symptoms of pollinosis appeared in this group later than in patients sensitive to grass pollen allergens only (over 21 years of age in 71%). bronchial asthma was ...19902098733
[food allergies 1983-1987].229 patients who showed an immediate-type allergy to one or more specific foodstuffs diagnosed from 1983 to 1987 at the allergy unit of the department of dermatology, zurich university hospital, have been studied. of these predominantly adult patients, 66 (28.8%) were male and 163 (71.2%) female. 70.7% of the food allergic patients also showed one or more atopic manifestations, in particular 53.3% a pollinosis. the food-induced symptoms included involvement of the skin (rashes, urticaria, angioe ...19911957142
allergen-specific leukocyte adherence inhibition (lai) assay: sensitivity, specificity and allergen-specific tube leukocyte adherence inhibition (lai) assay has been developed in order to study the mechanism by which leukocytes lose their normal property of adherence to glass. peripheral blood leukocytes (pbl) from 27 individuals allergic to dermatophagoides farinae (df), 10 with seasonal rhinitis not induced by df and 49 non-allergic healthy volunteers were challenged in vitro with df and a non-relevant allergen, artemisia vulgaris (av) and then assayed for the ability to adhere t ...19873323035
probit analysis applied to the allergen dose-response curve: a method for epidemiologic surveys.the degree of skin sensitivity to five common allergens (grass, dermatophagoides pteronyssinus, mugwort, birch, and parietaria) was determined by the threshold dilution technique in all the skin test reactors of a random sample of 295 schoolchildren (142 male and 153 female subjects, age range 11 to 14 years), and the frequency distribution of responders at each concentration was analyzed by probit analysis. the potency of each allergen was presented in terms of median effective dose (ed50), and ...19883339189
structural analysis of the glycoprotein allergen art v ii from the pollen of mugwort (artemisia vulgaris l.).the glycoprotein allergen art v ii, from the pollen of mugwort (artemisia vulgaris l.) was treated with peptide:n-glycosidase f (pngase f) to release asparagine-linked oligosaccharides. the oligosaccharides were isolated by gel permeation chromatography and their structures determined by 500-mhz 1h nmr spectroscopy, fast atom bombardment-mass spectrometry, and high-ph anion-exchange chromatography. the high-mannose oligosaccharides man5glcnac2, man6glcnac2, man7glcnac2, man8glcnac2, and man9glcn ...19911703533
aeroallergen analyses and their clinical relevance. ii. sampling by high-volume airsampler with immunochemical quantification versus burkard pollen trap sampling with morphologic quantification.a comparison was made between the amount of airborne pollen collected by burkard airsampler and the allergenic activity of particles trapped on glass fibre filters in an accu-vol high-volume airsampler. the comparison was made throughout the pollen seasons 1986 to 1989. both airsamplers were operated 24 h a day. they were placed less than 5 m apart, and estimation of the pollen amount was made on a day-to-day basis during the pollen seasons, and on a weekly basis outside the seasons. the occurre ...19921485654
[neglected allergens].the atopic symptoms manifest themselves mainly in hay fever or bronchial asthma. in a not inconsiderable number of cases, the question of oral complaints, connected with the intake of certain foodstuffs, is ignored in the medical anamnesis. here, we are dealing with what is known as food-associated allergy syndrome, which is largely based on a cross reaction between certain types of pollen (birch, alder, hazel and mugwort) and food allergens (drupes, pomes, nuts, vegetables such as celery, carro ...19921440434
profilins constitute a novel family of functional plant pan-allergens.type i allergy is a major health problem in industrialized countries where up to 15% of the population suffer from allergic symptoms (rhinitis, conjunctivitis, and asthma). previously, we identified a cdna clone that encoded a birch pollen allergen as profilin. profilins constitute a ubiquitous family of proteins that control actin polymerization in eukaryotic cells; in particular, profilin participates in the acrosomal reaction of animal sperm cells. although profilins had been unknown in plant ...19921370681
the most important allergens in allergic rhinitis.the distribution of allergens, found on thorough allergy work-up in a series of 770 patients with seasonal and perennial nasal symptoms, is reported. the percentages of positive reactions to grass, tree, and herb pollens were equally high (30--40%) but, clinically, pollens from grasses (timothy, alopecurus, kentucky blue and meadow fescue), birch and mugwort (artemisia) were the most important. in the nordic countries house dust is evidently the commonest causative agent in perennial rhinitis bu ...1979287333
viruses and airborne allergens as precipitants of obstructive respiratory difficulties in children.findings of virus infection were prospectively studied in 133 episodes of obstructive respiratory difficulty in 90 children aged 2 to 15 years. a viral infection was confirmed in 16 episodes (12%). in a further 11% of cases serological evidence of a recent viral infection was found. respiratory syncytial virus (rsv) accounted for half of the positive viral results. skin prick tests were performed after the second episode of bronchial obstruction. of the 79 children tested, 57% had at least one p ...19883218914
reproducibility of skin prick testing with allergen extracts from different manufacturers.allergen extracts for skin prick testing (spt) are available from several manufacturers. although these solutions are specified according to well-defined internal company standards, there is no generally accepted overall standardization. to assess the comparability of skin prick test solutions of various manufacturers, we compared extracts of nine different allergens from four companies by spt in 29 children sensitive to one or more of the allergens, in a double blind fashion. rast (pharmacia) a ...19883189725
[food allergies. iii. therapy: elimination diet, symptomatic drug prophylaxis and specific hyposensitization].the treatment of food allergies is logically based on strict elimination of causative allergens. while it is easy to eliminate food which is infrequently consumed, it is more difficult to manage an allergy involving regularly consumed foods, especially where patients have to eat away from home for professional reasons. the creation of elimination diets for milk, eggs, and mould and yeast allergies is discussed. in raw food and vegetable allergy the act of cooking is often sufficient to denature ...19863097814
[a clinical study of two cases with immediate hypersensitivity to apple-pulp and an investigation of cross-allergenicity between apple-pulp allergen and some other pollen allergens].in japan there are no case reports of immediate hypersensitivity to apple-pulp. here, we report on two patients: a 23-year-old woman and a 14-year-old girl with 3-year and 7-year histories, respectively, of anaphylactic reactions to apple-pulp. in the first patient, eating raw apples immediately elicited itching and tingling of the lips and mouth with severe edema of the lips and tongue, irritation of the throat and slight colic in the upper abdomen. in the second, nausea and vomiting occurred a ...19892818215
allergic conjunctivitis, total and specific ige in the tear fluid.twenty-three patients with allergic conjunctivitis caused by birch, timothy or mugwort pollen were examined for the presence of total and specific ige in the tear fluid. in thirteen of the 23 patients ige could be shown in the tears. specific ige antibodies against the causative pollens were present in the tear fluid of 8 of the patients. the relation between serum and tear ige on one hand and serum and tear albumin on the other hand suggests local production of the specific ige antibody.19853993353
risk factors for sensitization to furred pets.the risk factors for sensitization to pets was investigated in 169 male pupils. a recent or former contact with cats, dogs, and guinea pigs in own home was reported in 52 (30.8%), 42 (24.9%), and 20 (11.8%) study subjects, respectively. clinically manifested allergy was found in two probands to cats and in two others to guinea pigs, three of them had formerly had pets and one proband with allergic rhinitis to cats had never had any pet. sensitization to animals and aeroallergens was investigated ...19902309988
celery allergy: clinical and biological study of 20 20 patients, the ingestion of celery was responsible for mucocutaneous symptoms (generalized urticaria and angioedema) (18/20) and respiratory disorders (7/20). four cases of systemic anaphylaxis were observed. the main associated allergic disorder was pollinosis (16/20). food allergy to other vegetable products, mainly other umbelliferae and apples, coexisted with celery allergy in 12 cases. it was found that celery allergy is mediated by ige antibodies: it is easily diagnosed by skin tests ...19883258136
isolation and characterization of a glycoprotein allergen, art v ii, from pollen of mugwort (artemisia vulgaris l.).a glycoprotein allergen, art v ii, was isolated from pollen of mugwort by two different isolation procedures. art v ii-a was isolated by a combination of ion-exchange chromatography on deae-sepharose, affinity chromatography on con a-sepharose and ion-exchange chromatography on mono p. art v ii-b was isolated by a combination of preparative ief in ultrodex granulated gel, affinity chromatography on con a-sepharose and hplc size exclusion on ultropac tsk g2000sw. art v ii-a and art v ii-b were sh ...19902233755
results of biological standardization with standardized allergen preparations.the aim of biological standardization (bs) is to equilibrate the activity (potency) of allergen extracts from different source materials. this was done by performing skin prick tests (spt) on patients who were sensitive to one of the following 10 allergens: birch, alder, hazel, timothy, rye grass, velvet grass, cultivated rye, mugwort, d. farinae and cladosporium herbarum. patient sensitivity varied within a range of three to four powers of ten for each allergen investigated. the weal size in ea ...19873296840
spice allergy in celery-sensitive patients.scratch tests (sct) with powdered commercial spices were performed in 70 patients with positive skin tests to birch and/or mugwort pollens and celery. positive sct to aniseed, fennel, coriander and cumin--all from the same botanical family (apiaceae) as celery--were observed in more than 24 patients. spices from unrelated families (red pepper, white pepper, ginger, nutmeg, cinnamon) elicited positive immediate skin test reactions only in three of 11 patients. specific serum ige to spices (determ ...19911957997
[pollinosis. iv. which pollens should be tested in allergology practice? results of determinations of allergy-causing pollens in the zurich air 1981-1984, with reference to threshold concentrations].the most important pollens causing pollinosis in zurich include hazel (corylus), alder (alnus) and birch (betula) in spring, grasses--including rye--in summer, and mugwort (artemisia) in autumn. investigations on airborne pollen of zurich were conducted from 1981 to 1984, and assessed with special reference to threshold concentrations. the results show that sorrel (rumex) and plantain (plantago) also belong to the most important pollens and should therefore be included in the test spectrum for a ...19854048909
comparison of rumen microbial inhibition resulting from various essential oils isolated from relatively unpalatable plant species.essential oils were isolated from eight plant species which were relatively unpalatable to sheep and deer. the inhibitory potency of these essential oils upon sheep and deer rumen microorganisms was compared, in terms of total gas and volatile fatty acid (vfa) production, by use of an anaerobic manometric technique. inhibitory effects of oils from the eight plant species may be placed in four groups: (i) essential oils from vinegar weed (trichostema lanceoletum) and california bay (umbellularia ...19685636470
a preliminary pharmacological screening of artemisia vulgaris linn. 19685658501
[allergen analysis of mugwort pollen (artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition].it could be identified 43 antigens of mugwort pollen (artemisia vulgaris), 31 migrating anodically and 12 cathodically, by means of crossed immunoelectrophoresis (cie). eleven antigens bound human ige-antibodies in crossed radioimmunoelectrophoresis (crie). two major allergens could be found, allergens 23 and 40.- the allergenic activity of mugwort pollen extract was examined by means of rast-inhibition. specificity of this reaction could be determined by a control trial with grass pollen extrac ...19883400558
identification and characterization of important allergens from mugwort pollen by ief, sds-page and immunoblotting.mugwort (artemisia vulgaris l.) pollen allergens, separated by sds-page or ief, were identified after transfer to ncm by incubation with a panel of sera from 16 patients with clinical mugwort pollen allergy, followed by [125i]anti-ige and autoradiography. of the at least 23 components separated by sds-page in a 15% polyacrylamide gel, at least 15 components with mol. wts 12,000-100,000 bound ige from the panel of patient sera. a component of mol. wt 22,000 bound ige from at least 94% of the pati ...19911857351
[celery allergy (celery contact urticaria syndrome) and relation to allergies to other plant antigens].comparative study of 13 patients (group a) with severe attacks of angio-oedema and urticaria after eating celery and 143 atopics with hypersensitivity to mugwort-pollen (group b). due to the severity of the reactions (group a) no oral provocation tests were performed. scratch chamber tests with raw and cooked celery (and related plants) confirmed the case histories. rast answers to celery were compared for both groups. rast inhibition indicated that celery and mugwort-pollen and also celery and ...19836670280
purification and characterization of an allergen from celery immunochemically related to an allergen present in several other plant species. identification as a profilin.the purification of a 15 kd allergen from celery was obtained by a four step procedure. evidence for at least two isoallergenic forms was obtained after analysis by two-dimensional-electrophoresis. a rabbit polyclonal antibody raised against this purified allergen allowed us to confirm our precedent results on the occurrence of allergenically and molecular mass-related components in celery and birch and mugwort pollens. in addition such components were also present in numerous other species like ...19921525697
mechanism of allergic cross-reactions. v. high incidence of unanticipated cross-stimulation by natural allergens of rat basophilic leukemia cells sensitized with monoclonal ige antibodies.the incidence of cross-stimulations by natural allergens was investigated using rbl-2h3 cells sensitized with five different mouse monoclonal anti-dnp iges and four mercury-induced rat monoclonal iges. cells sensitized with 3 of the 5 monoclonal anti-dnp iges (clones spe-7, srt-1, lb4) responded by serotonin release upon stimulation by natural allergens such as dermatophagoides pteronyssinus, horse dander and mugwort extracts. serotonin release could be inhibited by monovalent dnp-lysine, indica ...19957549509
[classification of allergens by positive percentage agreement and cluster analysis based on specific ige antibodies in asthmatic children].classification and characterization of allergens is important because allergic patients are sensitized by a variety of allergens. one hundred and sixty-one sera from asthmatic children were investigated for specific ige antibodies against 35 allergens including 20 inhalants and 15 foods by means of the mast method. we assessed the allergenic properties of the allergens based on positive percentage agreement and cluster analysis. there was a high positive percentage agreement of specific ige anti ...19921482294
a study of allergens in celery with cross-sensitivity to mugwort and birch pollens.sixty-one sera with positive rast to mugwort pollen (artemisiae vulgaris) were submitted to rasts for birch pollen (betula verrucosa) and celery (apium graveolens). in 36 cases rast results were positive for celery. in addition, 23 sera presented specific ige to birch pollen. the binding of specific ige to individual allergens in celery, mugwort pollen and birch pollen was studied by the immunoblotting technique. this involved electrophoretic separation of allergenic extracts, electrotransfer of ...19883233726
survey of the allergic status of patients with bronchial asthma in turkey: a multicenter study.patients diagnosed with bronchial asthma (ba) were prospectively enrolled to assess their allergen spectra and atopic status. the patients came from five major cities (ankara, izmir, samsun, elaziğ, and adana) in different regions of turkey. atopic status, total ige levels, and allergen spectra were determined in 1149 patients and 210 controls who were spouses of the patients sharing the same environment but not consanguinity with the patients. total ige levels were significantly higher in the a ...19957573836
in vivo tests with pollen extracts previously investigated by means of direct rast titration allergen assay.the allergenic potency of different birch, timothy and mugwort pollen extracts was determined by means of a direct rast titration allergen assay. for birch and timothy allergens, the results of skin and provocation tests did not confirm the results of the in vitro determinations of allergenicity. there was a poor correlation between the results of skin tests and the results of phadebas rast for determination of specific ige to mugwort, whereas the correlation between skin tests and rast for othe ...1978709789
relationship between nasal and conjunctival tests in patients with allergic rhinitis.simultaneous conjunctival and nasal provcation tests, a total of 174 test pairs, were carried out in fifty patients with allergic rhinitis, using serially diluted antigen solutions of birch, timothy grass and mugwort pollen, as well as cat and dog dander. the nasal mucosa was found to be more sensitive than the conjunctival mucosa in ninety-six test pairs (55%). this differs from earlier reports. nasal reaction only was observed in twenty-nine instances (17%). posterior rhinomanometry was also u ...1978709788
[pollionosis: i. findings on the clinical aspects and the pollen spectrum in 1565 pollen-sensitive patients].case histories are analyzed of 1565 hay fever patients first attending an allergy unit. the mean age of the test persons was 19.5 years. 40% were in the age group 5 to 15 years. the sex distribution showed a slight but statistically significant prevalence of males (56.6%). 56.8% had a positive family history of allergies and 44.2% had other allergic conditions such as atopic dermatitis (31.6%), perennial rhinitis and perennial asthma (19% each), urticaria, food allergy and drug allergy (5% each) ...1979493910
causative allergens of allergic rhinitis in japan with special reference to silkworm moth determine the importance of silkworm moth allergens, we tested 267 patients with allergic rhinitis with cap-rast, a new assay system for detecting specific ige in sera. we used the following allergen sources: house-dust mite, candida, alternaria, cat dander, orchard grass, ragweed, mugwort, japanese hops, japanese cedar, japanese cypress, cotton, silk, larval chironomid midge, adult chironomid midge, and silkworm moth. as expected, the highest positive rate of reaction, as determined by cap-r ...19957741185
pollen seasons: forecasts of the most important allergenic plants in finland.occurrence of airborne pollen in finland has been studied for 10 years in turku (southern finland), 8 years in oulu, 4 years in kuopio (central finland) and 7 years at kevo (northern lapland). observations on the pollen seasons of alder, birch, grasses and mugwort are presented. all these pollens occur in south and mid- finland in quantities capable of causing allergy symptoms. except for birch pollen, allergenic pollens occur in far lower concentrations than in central europe. in northern lapla ...19863752415
allergens of chrysanthemum pollen.chrysanthemum pollen extracts analyzed by means of crossed radioimmunoelectrophoresis (crie) and rast-inhibition. thirty-eight antigens were identified and out of them 10 were able to bind ige antibodies from sera of patients with pollinosis elicited by these pollens. an allergenic relationship of chrysanthemum pollen to mugwort pollen was detected in the rast-inhibition experiments.19863962817
the sensitizing capacity of compositae plants. vi. guinea pig sensitization experiments with ornamental plants and weeds using different methods.experimental studies in guinea pigs using ether extracts of 20 different species of the compositae plant family were carried out with the open epicutaneous method (oet) and the guinea pig maximization test (gpmt). the results demonstrate that cnicus benedictus (blessed thistle), chrysanthemum leucanthemum (marguerite, ox-eye daisy) and helianthus debilis (dwarf sunflower) are strong sensitizers while helenium amarum (bitterweed), gaillardia amblyodon (blanket flower), artemisia ludoviciana (prai ...19853970585
kiwi fruit allergy: a new birch pollen-associated food determine the cross-reacting antigens of kiwi fruit and other foods and pollen, we investigated 22 patients allergic to kiwi fruit: 10 with severe systemic reactions and 12 with localized symptoms confined to oral and pharyngeal mucosa (oral allergy syndrome). seven patients with birch pollen allergy who tolerated kiwi fruit were included as a control group.19948027500
ige to food allergens are highly prevalent in patients allergic to pollens, with and without symptoms of food allergy.serum samples from 274 patients allergic to one or more of three pollens (birch, grass, mugwort), from 36 patients allergic to cat and/or dermatophagoides pteronyssinus but not to pollen and from 55 non-allergic controls, as well as 20 cord blood samples, were examined for specific ige to six 'pollen-associated' food allergens by using a new sensitive assay (cap). a questionnaire asking for reactions to food was also sent to all patients. in the pollen group, 111 patients (47%) were positive (> ...19948039023
skin disorders and prick and patch test reactivity in finnish reindeer addition to an interview, skin tests and clinical examinations were performed on 211 randomly selected reindeer herders from 21 to 77 years of age (mean 45 years). skin tests consisted of skin prick tests (spt) with 9 allergens: cat and cow epithelium, dog, horse and reindeer epithelium, house dust mite, birch pollen, meadow grass pollen and mugwort pollen, as well as patch tests with 31 allergens. of the 211 tested, 19 (9%) had positive spt reactions to at least one allergen, and 19/173 (11% ...19882970375
comparison of allergic rhinitis and vasomotor rhinitis patients on the basis of a computer questionnaire.from 1 july 1990 to 31 december 1991, all patients referred to the allergy section of the ent department, university hospital, lund, sweden, (n = 678) answered a 134-item questionnaire presented on the screen of a personal computer by pressing y (for yes) or n (for no) on the keyboard. the objective of this study was to compare the questionnaire responses from patients with allergic rhinitis (ar) with those of patients with perennial nonallergic rhinitis or vasomotor rhinitis (vmr). nasal blocka ...19938116859
allergenic pollens in the southern mediterranean the mediterranean area there are characteristic climatic conditions (mildness of winter, summer dryness, etc.) that facilitate the growing of a typical vegetation with production of allergenic pollen, such as those from parietaria and olea europaea, very different from that of central and northern europe. we present in this article the results of an 8-year pollen count in the atmosphere of naples, italy. the pollen content was examined with a volumetric spore trap (lanzoni vpps-2000) like tha ...19892913131
evaluation of the overall degree of sensitization to airborne allergens by a single serologic test: implications for epidemiologic studies of most epidemiologic studies of respiratory allergy, the overall degree of sensitization is usually measured by indices based on skin prick test reactions to a panel of relevant airborne allergens. in the present study we used a single assay (cap-phadiatop, pharmacia, uppsala, sweden), which is based on the reaction between serum-specific ige and a mixture of locally relevant inhalant allergens to measure the degree of sensitization to inhalants in the sera of 1815 young italian men, in whom a ...19948308184
immunological investigation of possible structural similarities between pollen antigens and antigens in apple, carrot and celery tuber.crossed line immunoelectrophoresis (clie) was used to show that a minor allergen present in birch pollen and another present in timothy pollen shared common epitopes with antigens in apple, carrot and celery tuber. major pollen allergens were not involved. structural similarities were also noticed in some mugwort pollen antigens and antigens in apple, carrot and celery, but none of these mugwort antigens acted as an allergen. in crossed immunoelectrophoresis (cie) of samples of apple, carrot or ...19854003724
[hypersensitivity to weed pollen allergens in the region of bygdoszcz].case records of 8576 patients suffering from hypersensitivity symptoms within upper airways, who were treated at the otolaryngological section of the allergological outpatient department of medical academy in bydgoszcz in the years 1975-1989, were analysed. pollinosis was diagnosed through anamnesis, physical examination and skin tests in 2561 (29.9%) individuals, i.e. 1153 women and 1408 men. hypersensitivity to weed pollen allergens was found in 1069 (12.5% of all people examined) patients wit ...19938401342
screening of taiwanese crude drugs for antibacterial activity against streptococcus mutans.preliminary antibacterial screening of local crude drugs was carried out using the cariogenic bacterium, streptococcus mutans. of 79 aqueous extracts tested, 6 crude drugs were shown to have significant antibacterial activity with minimal inhibitory concentration equal to or lower than 7.8 mg/ml (expressed in terms of dry starting material). of these effective crude drugs, morus australis, ludwigia octovalvis and thuja orientalis were very effective in inhibiting the growth of serotypes c and d ...19892615434
the use of a reference allergenic extract in the evaluation of allergen products.two commercially available mugwort (artemisia vulgaris (av] pollen extracts av-alk and av-ps were compared with a dialysed reference mugwort pollen extract (av-ref) by means of crossed immunoelectrophoresis (cie), crossed radio immunoelectrophoresis (crie), rast-inhibition, rast-screening and skin prick test. av-alk and av-ps both contained approximately half the number of precipitation arcs detectable in av-ref, and av-ps seemed to lack two intermediate allergens and two minor allergens, while ...19854003725
celery sensitivity: clinical and immunological correlations with pollen allergy.the authors studied twenty patients with celery allergy and concomitant hypersensitivity to certain pollens (mugwort, birch). the specific symptoms induced by eating celery were attacks of urticaria and angio oedema (seventeen out of twenty) respiratory complaints (eight out of twenty), systemic anaphylaxis with vascular collapse (three out of twenty). a strong association between clinical reactions to celery and mugwort sensitization, and to a lesser degree between celery allergy and birch poll ...19854006177
multicentre evaluation of capture assay radim liquid allergen for measurement of specific ige antibodies.a multicentre trial of capture assay radim liquid allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. the results of the evaluation were compared with clinical data and in vivo testing. good agreement was obtained for dermatophagoides pteronyssinus (d1), cat's epithelium (e1), betula verrucosa (t3) and olea europea (t9), artemisia vulgaris (w6) and parietaria officinalis (w19). some spreading of data was observed for art ...19958608200
[immediate hypersensitivity to fruits and vegetables and pollenosis].various works have been published, mainly by scandinavian authors, in which a partial immunological identity has been found to exist between birch pollen and hazelnut and apple. however, our attention has been particularly drawn to the high proportion of people who are allergic to the apple and other fruits, which in some cases has been as much as 50 and 70%, and in whom an oral pruritus alone is considered sufficient a symptom of allergies to certain foodstuffs. however due to the fact that the ...19854036764
skin prick tests with standardized extracts of inhalant allergens in 7099 adult patients with asthma or rhinitis: cross-sensitizations and relationships to age, sex, month of birth and year of testing.the results of skin prick tests (spts) performed between 1981 and 1992 on 7099 adult patients with asthma and/or rhinitis were retrospectively analyzed. standardized soluprick extracts of dermatophagoides pteronyssinus, animals and pollen, and unstandardized extracts of cladosporium++ and chironomids (red mosquito larvae, chir), were used. the proportion of atopics (patients with positive spt results) was 44 percent, decreasing from 61 percent in patients 14-20 years old to 18 percent in patient ...19968833167
hypersensitivity to carrot associated with specific ige to grass and tree pollens.this study deals with a 34-year-old female cook with no previous history of atopy, who was studied because of allergic rhinoconjunctivitis and contact urticaria in both hands associated with severe itching when she handled raw carrot. the patient had had anaphylactic episodes after accidental ingestion of raw carrots, but she tolerated cooked carrots. skin prick tests with carrot, celery, and olive, and birch, grass, and mugwort pollens were positive. total ige was 411 ui/ml. specific ige to oli ...19968837668
allergens in pollen from mugwort (artemisia vulgaris l.). i. partial characterization of allergen preparations from mugwort pollen with emphasis on the carbohydrate moiety.the results obtained in these studies show that certain mugwort allergens are extracted after 5 min extraction time and that there is little difference whether phosphate-buffered saline or nh4hco3 is used as extraction buffer. an extraction time of 1 h appears to be sufficient for a quantitative extraction of the main part of the allergens present. when the extracts are dialyzed, cathodic antigens/allergens are lost. addition of thiourea leads to a reduction in the coloured matter of the extract ...19854044052
inhalative occupational and ingestive immediate-type allergy caused by chicory (cichorium intybus).we report a first case of occupational allergy to chicory (cichorium intybus) in a vegetable wholesaler. symptoms occurred after oral, cutaneous or inhalatory exposure. the patient also reported reactions after ingestion of botanically related endive (cichorium endivia) and lettuce (lactuca sativa). we identified the responsible allergen by sds-page and immunoblot to be a 48-kda protein, confined to the non-illuminated parts of the plants. no cross-reactivity was found with mugwort (artemisia vu ...19968877160
sensitization to neumoallergens influence in mothers of asthmatic children.allergy diseases in our province have the characteristic of early appearance of asthma and predominant monosensitization to domestic dust mites due to the climatic condition. in this work we study the influence in allergic children. we selected 1028 mothers that brought their children to hospital for the first time. we divided them in two groups. one was comprised of 459 mothers of atopic children (ma) and another of 569 mothers of non atopic children (mc). all mothers were skin-prick tested (ph ...19968882754
cross-reactivity of ige antibodies with allergens in birch pollen, fruits and vegetables.allergens extracted from apple, carrot, celery tuber and orange were coupled to radioallergosorbent test (rast) discs. these and commercial birch, mugwort and timothy pollen discs were used to study sera from 90 patients for the presence of ige antibodies which react with the allergens in question. individual sera frequently gave positive results with several discs - in particular with birch pollen, apple, carrot and celery tuber discs. if ige antibodies to birch pollen or apple allergens were d ...19846609888
birch pollen-related food hypersensitivity: influence of total and specific ige levels. a multicenter ige, rast results with tree pollen allergens, and prick test results with birch, grass and mugwort pollen allergens were correlated to 872 hay fever patients' reported food hypersensitivity (fh). a positive correlation was found between fh and the rast and prick test results with birch pollen allergen. at each level of birch pollen sensitivity the incidence of fh was lower in patients with high total ige than in those with lower total ige. a negative correlation was found between grass pol ...19836614407
identification of a 60 kd cross-reactive allergen in pollen and plant-derived food.cross-reactive ige antibodies were found to be responsible for allergic reactions in patients allergic to pollen on ingestion of food (oral allergy syndrome). so far, the major birch pollen allergen bet v 1 and birch profilin (bet v 2) were identified as relevant cross-reactive allergens.19968939157
comparison of multiple-antigen simultaneous test and cap systems for diagnosis of nasal allergy.the multiple-antigen simultaneous test (mast) is a simple system that uses no radioactive agents and allows simultaneous examination of multiple antigens. cap is a quick new, in vitro system that is more sensitive than the radio-allergosorbent test (rast). to evaluate their clinical efficacies, we examined the correlation between the mast and cap systems. serum samples were collected from 33 patients with nasal allergies, 13 males and 20 females, mean age 31.1 years. the mast and cap were used f ...19957478456
[rules concerning allergy to celery (and other umbellifera)].1. allergy to celery is associated with a pollinosis. even if there are no overt symptoms of the pollinosis there are positive tests, including rast. 2. the causative pollen is usually the most common of the region, for example in sweden it is birch (betula), whilst in the surroundings of lyon in france ragweed (ambrosia) and mugwort (artemisia) and compositae in general seem to play a particularly important role. 3. the allergy is strictly on-way, celery-pollen. if the reverse should occur the ...19873454174
atopic sensitization of dairy farmers to work-related and common allergens.atopic sensitization was studied using skin tests on 93 randomly selected, non-smoking dairy farmers. the farmers lived in the municipality of pielavesi, in eastern finland. the reference group consisted of 84 non-smoking teachers randomly selected from all the teachers employed by the city of kuopio, which is in the same administrative district as pielavesi. thirty-four allergens were included in the test panel. for testing these allergens, we used the prick technique with disposable precision ...19873478214
[relationship between birch pollen allergy and oral and pharyngeal hypersensitivity to fruit].we evaluated the relationship between birch pollen allergy and oral and pharyngeal hypersensitivities to certain fruits. 1. of 171 birch pollen cap positive (score > or = 2) patients, twenty two (13%) were revealed to be hypersensitive to apples, eleven (6%) to be hypersensitive to peaches, both rates being higher than those found in patients with other cap positive reactions (orchard grass pollen cap, mugwort pollen cap or dermatophagoides pteronyssius cap positive). 2. among the birch pollen c ...19957562228
mercurialis annua: characterization of main allergens and cross-reactivity with other species.a multicentric study was conducted to evaluate the frequency of mercurialis annua pollen sensitization in several areas of spain and to select a population sample to characterize the main allergenic components in m. annua pollen. patients were recruited from six hospitals in spain. out of 420 patients sensitized to pollens, 195 (46.4%) showed positive skin tests to m. annua, thus evidencing the high level of sensitization to the pollen of this plant in spain. thirty-seven sera with rast class va ...19979104791
oral allergy syndrome to a jackfruit (artocarpus integrifolia).a 30-year-old man from the philippines with pollen allergy noted the appearance of oral allergy syndrome (oas) after eating raw apple, raw peach, raw celery, and recently, jackfruit (artocarpus integrifolia), a tropical fruit which belongs to the moraceae family (mulberry) and to the genus artocarpus (breadfruit tree). despite the patient's multiple sensitization in skin prick tests and in the pharmacia cap system to birch, grass, mugwort pollen, related fruits and vegetables, and jackfruit, in ...19979188925
natural latex, grass pollen, and weed pollen share ige epitopes.because of the frequent use of natural latex products, ige-mediated reactions to latex proteins represent an important health threat in industrialized countries. although several latex allergens have been characterized and ige cross-reactivities with allergens present in plant-derived food have been described, limited information is available regarding the presence of common ige-binding components in latex and plant pollen.19979314348
[so-called "celery-carrot-mugwort-spice syndrome." rast results with new spice discs].rast investigations on the sera of 27 patients suffering from celery allergy showed specific ige to mugwort and birch in 15 cases; sensitization to mugwort or birch alone only occurred in 5 and 7 cases, respectively. positive rast results were also obtained with several spices. we found a relationship between spice and celery allergy.19873590917
allergy screening in asthma and allergic rhinitis. which allergens should be used?to detect atopy by a screening method employing skin prick testing with a limited number of allergens, the test results of 939 patients with allergic airways diseases were analysed. it was found that an allergen panel consisting of cat, timothy and house dust mite could detect 85% of atopic patients with asthma and/or rhinitis. for subgroups of patients the results were even more favourable. thus 98% of atopic patients with seasonal allergic rhinitis were detected by an allergen panel consisting ...19873592143
quantitative skin prick testing. dose-response of histamine- and allergen-induced wheal reactions.dose-response curves of histamine- and allergen-induced wheal areas were evaluated in seven normals (defined as negative skin prick test (spt) to inhalant allergens and no clinical signs of allergy), seven latent allergics (positive spt without allergic symptoms), and 20 manifest allergics (positive spt and allergic symptoms). three concentrations of histamine hcl (1, 10 and 100 mg/ml) and three 10-fold concentrations of nine inhalant allergens (birch, timothy, mugwort, horse, dog, cat, house du ...19873592144
antigenic relationship between mugwort and ragweed pollens by crossed immunoelectrophoresis.mugwort and ragweed pollens have been considered as important respiratory allergens in korea. these two pollens are abundant in the air of seoul from august through october. many ragweed-sensitive patients have shown concurrent sensitivities to mugwort pollen. however the antigenic relationship between these two pollens has not been clarified. to observe the cross-reactivity between them, we developed polyclonal anti-mugwort and anti-ragweed antibodies by immunization on new zealand white rabbit ...19947993587
identification of common allergenic structures in mugwort and ragweed pollen.identification of common allergenic structures in mugwort and ragweed pollen.19989500752
banana allergy in patients with immediate-type hypersensitivity to natural rubber latex: characterization of cross-reacting antibodies and association between allergy to latex and banana has been reported. even though cross-reacting ige antibodies have been demonstrated, in no study has the existence of structurally similar allergens been confirmed. in the present study banana allergy was studied in a large series of patients with latex allergy. specific ige antibodies were characterized for cross-reactivity and compared with pollen rast results. latex and banana extracts were investigated for common antigens and allergens.19948006321
[the incidence of peculiar ige in response to inhalant allergens in relation to the age of the patient].208 patients between the age of 1 month to 56 years, with symptoms of respiratory tract allergy were investigated. in all cases serum titre of sige to birch, thymoty, rye, mugwort pollen, alternaria alternata, dog, cat fur, dermatophagoides pteronyssinus and farinae was determined with the use of quidel immunoenzymatic method. we have found sige to alternaria alternata in 8 (38%), birch pollen in 5 (23.8%) dog fur in 5 (23.8%) and thymoty and rye pollen in 3 (14.3%) even among one years-old infa ...19979518328
prevalence of atopy in students from málaga, spain.epidemiologic studies are necessary to determine the prevalence of allergic diseases. this varies widely depending on allergen preparations and patients studied.19989532972
hypersensitivity to parietaria officinalis pollen in newcomers to the area with the plant.hypersensitivity to parietaria officinalis (wall pellitory) pollen and other environmental allergens was studied in pollinosis patients allergic to p. officinalis pollen who were born in areas without p. officinalis and later moved to the city of split, where p. officinalis is responsible for some 65% of pollinosis cases. highly significant positive correlations were found for both the intensity of skin test reaction and concentration of specific serum ige with the length of residence in the are ...19938116858
[allergic reactions of the respiratory tract].allergic rhinoconjunctivitis and allergic asthma bronchiale are immunologically mediated diseases of the respiratory tract. dependent on the allergen, they occur seasonally or perennially. allergens can cause a specific reaction of the immune system, mediated by ige-antibodies in individuals with a genetic predisposition. different mediators are released from mast cells, and complex immunopathological mechanisms lead to inflammatory changes of the mucosa. a significant increase of allergies has ...19948146810
validation of a rhinitis symptom questionnaire (isaac core questions) in a population of swiss school children visiting the school health services. scarpol-team. swiss study on childhood allergy and respiratory symptom with respect to air pollution and climate. international study of asthma and allergies in childhood.the primary aim of the study was to assess the validity of the isaac core questions on rhinitis in a population of swiss school children by comparing them to skin prick test results. second, the positive predictive value in detecting atopy among children with rhinitis symptoms was determined. third, agreement between parental reports of hay fever and rhinitis symptoms was evaluated, since earlier swiss prevalence surveys had exclusively relied on reported hay fever.19979617776
immunoelectron-microscopic localization of ige binding site of mugwort elucidate the ige binding site of mugwort (artemisia vulgaris r.) pollen, pollen grains were frozen and fixed using a cryocut. they were incubated with antibodies according to the following sequence: sera pool of individuals who showed mugwort-rast class 3 or 4, biotin-labeled goat anti-human ige antibody, streptavidin-peroxidase and diaminobenzidine. then, they were observed under electron microscopy. the control section was incubated with the sera pool from individuals who showed a negative ...19938343219
identification of allergens in oilseed rape (brassica napus) pollen.pollen from oilseed rape (osr), brassica napus, an increasingly cultivated oilplant from the brassicaceae, has been recognized as a potential cause of allergic sensitization. allergens have been hardly investigated.19989784653
reactivity of carrot-specific ige antibodies with celery, apiaceous spices, and birch pollen.although carrot allergy is not well recognized in north america, the celery-carrot-mugwort-spice syndrome is well known in europe. in the current study, cross reactivity of carrot, stalk celery, and spices, all members of the apiaceae and birch pollen was assessed with serum from a patient reporting raw carrot-induced, raw stalk celery-induced, or spice-induced laryngeal edema and bronchospasm. by rast inhibition, some cross-reactivity was demonstrated between raw carrot and stalk celery and oth ...19938507047
a double-blind, randomized study investigating the efficacy and specificity of immunotherapy with artemisia vulgaris or phleum pratense/betula verrucosa.this study investigated the specificity and efficacy of immunotherapy with artemisia vulgaris (artemisia) extract. we studied 25 patients with a more than two year lasting history of seasonal rhinoconjunctivitis and who had only two clinically important allergies, either to artemisia and betula verrucosa (betula) or to artemisia and phleum pratense (phleum). patient selection was randomized and evaluation of results was conducted in double blind fashion. twenty patients completed two years of sp ...19958526170
pollen calendar of the city of salamanca (spain). aeropalynological analysis for 1981-1982 and 1991-1992.we report a study on the contents of airborne pollen in the city of salamanca (spain) aimed at establishing a pollen calendar for the city for the yearly periods of maximum concentrations, relating these with quantifiable atmospheric variables over two two-year periods with an interval of 10 years between them: 1981-82 and 1991-92. the pollen was captured with burkard spore-traps, based on hirst's volumetric method. determinations were made daily and were used to make preparations, previously st ...19989885728
environmental priming influences allergen-specific nasal reactivity.preceding mucosal response to one allergen leads to the priming of the nasal mucosal response to another allergen. this study aimed to determine whether environmental allergens, especially ubiquitous animal dander, can induce nasal priming.19989930593
evaluating the relevance of aeroallergen sensitization in atopic eczema with the atopy patch test: a randomized, double-blind multicenter study. atopy patch test study group.ige-mediated sensitization of questionable clinical relevance is routinely demonstrated in patients with atopic eczema (ae) by skin prick test or radioallergosorbent test (rast). we report the results of a randomized, double-blind multicenter study in patients with ae on the relevance and technical aspects of aeroallergen patch testing (atopy patch test, apt).199910025743
[phytoperfusion of the bladder after adenomectomy].for continuous irrigation of the bladder after prostatic adenomectomy herb infusion (urtica dioica l. 12-15 g/l, hypericum perforatum l. 8-12 g/l. marticaria recutita l. 8-10 g/l, folia plantaginis majoris 7-10 g/l, herba millefolii 4-6 g/l, folia betula 3-5 g/l. artemisia vulgaris l. 1-2 g/l, folia fragaria vesca 3-4 g/l, water 11 maximum) was dripped through a thin suprapubic and urethral drainages. such phytoperfusion of the urinary bladder used in 22 patients reduced postoperative blood loss ...19958571476
inhalant allergy to fresh asparagus.two patients experienced itching conjunctivitis, running nose, tightness of the throat and coughing during preparation of fresh asparagus. eating asparagus after cooking did not provoke any allergic symptoms. both patients were atopic, sensitized additionally to pollens of grasses and trees as well as to onion.19968729671
ubiquitous structures responsible for ige cross-reactivity between tomato fruit and grass pollen allergens.the simultaneous presence of ige reactivity to tomato fruit and grass pollen allergens is evident in many patients with allergy and may be caused by cross-reactivity. using sera from polysensitized patients with a positive enzyme allergosorbent test (east) result (score > 2), we tested reactivity to both allergen sources. ige reactivity against both extracts was demonstrated in eight serum samples, and cross-reactivity was confirmed by the east inhibition assay. the structures responsible for th ...19968876557
ige cross-reactivity between birch pollen, mugwort pollen and celery is due to at least three distinct cross-reacting allergens: immunoblot investigation of the birch-mugwort-celery syndrome.allergy to celery is often associated with sensitization to birch and/or mugwort pollen. objective and19968911702
allergic sensitization to native and heated celery root in pollen-sensitive patients investigated by skin test and ige binding.the rates of sensitization and allergy to four birch pollen related plant foods were investigated in a group of 167 patients who were sensitive to at least one kind of pollen and one particular food. sensitivity was concluded from a positive skin prick test or the determination of specific ige, whereas allergy was based on anamnestic data. the positivity rates for sensitization and allergy, respectively, were: apple, 93 and 84%; hazelnut, 90 and 78%; celery, 70 and 14%; carrot, 60 and 37%. compa ...19968917122
historical background of aeropalynology in the canary islands.this paper forms part of a doctorate thesis, currently being completed at the allergy and immunology section of the ntra. sra. de la candelaria hospital, tenerife, regarding the epidemiology of pollens we would like to illustrate that this island and its provinces are similar to a miniature continent, where pollens from the most important allergenic families present in the national territory of the iberian peninsula can be found. we have a very high incidence of patients allergic to grass pollen ...19969010563
anaphylactic reaction to young garlic.garlic is well known to cause contact dermatitis and asthma. however, it is a very rare cause of food allergy. we present the case of a 23-year-old woman with previous history of allergy to pollen and dried fruit, and food-dependent, exercise-induced anaphylaxis for which no specific food could be identified as responsible, who experienced an anaphylactic reaction after eating young garlic.199910435479
skin prick tests and in vitro immunoassays with native spices and spice prick tests of native spices (commercial powdered spices) are common in patients with allergy to birch or mugwort pollen. clinical symptoms from spices are infrequent but occasionally severe.19957552932
serological characterization of allergens in poppy seeds.poppy seeds in food can induce immediate-type allergic reactions ranging from mild local symptoms to severe anaphylactic reactions. previous publications showed that poppy seeds cross-react with other plant-derived allergens. the ige-binding components have not been defined so far.199910457111
identification and characterization of the major allergen of the humulus japonicus pollen.pollen of humulus japonicus has been known as one of the important causes of pollinosis in korea and china. to date, the major allergen of h. japonicus has not been determined.199910457112
molecular characterization of api g 1, the major allergen of celery (apium graveolens), and its immunological and structural relationships to a group of 17-kda tree pollen allergens.individuals suffering from immediate hypersensitivity (type-i allergy) to a particular pollen frequently display intolerance to several foods of plant origin. in this respect, individuals sensitized to birch pollen and/or mugwort pollen frequently display type-i allergic symptoms after ingestion of celery. in this study, we expressed the major allergenic protein of celery, api g 1, which is responsible for the birch-celery syndrome, in the form of a non-fusion protein. the open reading frame of ...19957588792
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