| the amino acid sequence of ragweed pollen allergen ra5. | the complete amino acid sequence of ra5, a ragweed pollen allergen, has been determined. allergen ra5 is a low molecular weight protein of 45 residues derived from ambrosia elatior, the short ragweed. it contains no detectable carbohydrate or lipid and has four disulfide bridges. the total structure was determined on 1.4 mumol of material and indicates that structural analysis is increasingly possible on relatively small amounts of highly purified material when a combination of automated and man ... | 1975 | 1122277 |
| studies on the deterioration of antigen e in extracts of ambrosia elatior. | five per cent (w/v) extracts of defatted ambrosia elatior pollen were prepared in bicarbonate-buffered saline (coca's extract) and coca's extract with 50% glycerol (gly-coca's extract). sterile extracts were stored in untreated glassware and glassware coated with silicone. stock extracts were diluted in phosphate-buffered saline (pbs) and stored at 5 +/- 3 degrees c. extracts 1:20, 1:40, and 1:400 were assayed at approximately 6-wk intervals for antigen e. quantitative determinations were made b ... | 1976 | 1245682 |
| [evaluation of the potency of the allergen preparation ambrosia elatior using a skin test and the inhibition radioallergosorbent test]. | the total allergenicity of a pollen extract ambrosia elatior was measured by quantitative prick test applied to the skin of seven highly sensitive patients and by standardized method of rast-inhibition. five 10-fold dilutions of the allergen concentration together with histamine hydrochloride 1 mg/ml (h1) were used. the induced wheal diametres for each concentration in per cent of the patient's own histamine wheal, were calculated. there was highly significant correlation (r = 0.9951) between th ... | 1992 | 1343028 |
| [skin hypersensitivity: intradermal hypersensitivity to allergen extracts and its relation to the place of residence]. | a retrospective review of 308 clinical files of patient to respond at immunological and clinical allergic service of the centro medico 20 de noviembre, issste for suffer with allergica illnes, realized by aeroalergens sensitization and determination frequency (intradermoreactions mediated) and relation to area living. the patients was highly sensitive at dust and dermatophagoides (75 and 40% respectively) others aeroalergen caused sensitization in the capriola dactylon (37%), amarantus palmieri ... | 1992 | 1492194 |
| purification and characterization of an allergen from celery immunochemically related to an allergen present in several other plant species. identification as a profilin. | the purification of a 15 kd allergen from celery was obtained by a four step procedure. evidence for at least two isoallergenic forms was obtained after analysis by two-dimensional-electrophoresis. a rabbit polyclonal antibody raised against this purified allergen allowed us to confirm our precedent results on the occurrence of allergenically and molecular mass-related components in celery and birch and mugwort pollens. in addition such components were also present in numerous other species like ... | 1992 | 1525697 |
| [biological standardization of the allergenic extract of ambrosia elatior]. | allergenic activity of allergen extract ambrosia elatior (ae) was tested in fifteen volunteers extremely sensitive to the allergen. the research was performed by using the quantitative prick (p) and the intradermal (id) test. the results were analyzed by the parallel line assay method. allergenic activity of the specimen was estimated: by the number of the allergen units (au) in 1 ml of the undiluted specimen according to the results of the p (prick) test; by the number of the allergen units (au ... | 1991 | 1766999 |
| [spread of ambrosia elatior in rural areas (investigation performed during the years 1985 and 1986 in southern drome)]. | the settings of ambrosia development are not only construction zones, waste grounds, road works; a country extension has been observed; more and more varied lands under cultivation (cereals, sunflowers, peach-trees...) are overgrown and the advance, toward south drome and ardèche since few years, clearly indicates that all the rhone valley is under threat. several forms of spreading are possible, sometimes very unexpected, so, for instance, the irrigation by spraying. the struggle must associate ... | 1987 | 3454180 |
| allergenic activity of short ragweed pollen (ambrosia elatior) from different years and/or suppliers: criteria for the selection of an in-house allergen reference preparation. | we have investigated dialyzed extracts (a-f) of short ragweed pollens (ambrosia elatior) from six consecutive production years and four different companies by protein nitrogen units, high performance liquid chromatography, isoelectric focusing, sds-page, rast inhibition, crossed radioimmunoelectrophoresis, and antigen e contents. extract a showed lowest yields in all quantitative assays. isoelectric focusing, sds-page, crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis showed ... | 1987 | 3592312 |
| aqueous-organic solvent extraction of water-insoluble protein from ragweed pollen. | a water-insoluble material was obtained from either short ragweed (ambrosia elatior) or false ragweed (franseria acanthicarpa) pollen extracted by organic solvent (50% n,n-dimethylformamide). further investigation of this water-insoluble material showed it contained at least five proteins. two of them were isolated and shown to possess antigenicity as well as allergenicity. rfa2 contains carbohydrate and is a glycoprotein. rfb2 not only possesses antigenic determinants found in the water-soluble ... | 1987 | 3652778 |
| [studies on the constituents of pollen. ii. on the constituents of pollen grains of ambrosia elatior l. (1) (author's transl)]. | | 1974 | 4859128 |
| antigens of ambrosia elatior (short ragweed) pollen. iii. crossed radioimmunoelectrophoresis of ragweed-allergic patients' sera with special attention to quantification of ige responses. | short ragweed allergenic extract has been studied by means of crossed radioimmunoelectrophoresis (crie) with the use of sera from 37 allergic patients and the relevant control sera. in this study 22 of 52 antigens, detectable in crossed immunoelectrophoresis (cie) against polyspecific rabbit anti-ragweed igg, were able to bind specific human ige to their corresponding immunoprecipitates. this binding was semiquantified by comparison with the binding of a standard serum pool. nine antigens were i ... | 1983 | 6217252 |
| isolation and properties of a new short ragweed pollen allergen, ra6. | two chromatographic forms (a and b) of the rapidly released short ragweed (ambrosia elatior) pollen allergen ra6 were isolated to antigenic ultrapurity by a combination of membrane filtration and ion exchange and gel filtration chromatography. both ra6 forms had a m.w. of 11,500 by gel filtration chromatography and 8000 by sds-page, and each consisted of two cathodically migrating bands on agarose electrophoresis at ph 8.5. amino-terminal amino acid sequencing analysis detected two similar seque ... | 1983 | 6863927 |
| two isoallergens of short ragweed component ra5. | two isoallergenic forms of short ragweed (ambrosia elatior) allergen ra5 were isolated in ultrapure form by a combination of molecular sieving (through amicon hollow-fiber cartridges), cation-exchange chromatography, and gel filtration. the two forms, ra5a and ra5b, exhibited charge differences by cm-sephadex chromatography and agarose electrophoresis at ph 8.5. the a form as identical to conventional ra5 by its physical and chemical properties and was more cationic than the previously undescrib ... | 1982 | 7086126 |
| antigens of ambrosia elatior (short ragweed) pollen. ii. immunochemical identification of known antigens by quantitative immunoelectrophoresis. | | 1981 | 7252152 |
| amino acid sequence of ragweed allergen ra3. | the complete amino acid sequence of ragweed pollen allergen ra3 has been determined. the molecule consists of 101 amino acid residues and to date is the only allergen isolated from ambrosia elatior (short ragweed) which contains carbohydrate. this particular preparation of allergen has a single, unique amino acid sequence, but there is evidence suggesting that, like ra5, ra3 isolated from pollen collected in diverse geographical areas shows amino acid sequence variation. the complete amino acid ... | 1980 | 7459340 |
| rapidly released allergens from short ragweed pollen. ii. identification and partial purification. | we have identified several basic allergens in 16-min extracts of short ragweed (ambrosia elatior) pollen and obtained a partially purified fraction (g50 ii) using a combination of ion-exchange (cm-sephadex c25) and gel-filtration (biogel p30 and sephadex g50) procedures. g50 ii was allergenically and antigenically distinct from known allergens antigen e, ra3, and ra5. it gave a highly symmetrical peak on sephadex g50 (fine), corresponding to a molecular weight of 11,500 daltons, and a single ban ... | 1981 | 7462538 |
| antigens of ambrosia elatior (short ragweed) pollen. i. crossed immunoelectrophoretic analyses. | a crude extract of short ragweed pollen was obtained by extraction, centrifugation, dialysis and freeze-drying. crossed immunoelectrophoresis, with a high-titer purified rabbit antibody fraction, revealed that the extract contained at least 52 antigens, of which 33 migrated toward the anode, 16 toward the cathode, and 3 toward both the anode and cathode. the electrophoretic precipitation pattern indicated a great deal of electrophoretic heterogeneity in several of the antigens. the overall distr ... | 1981 | 7462638 |
| ige-binding trypsin inhibitors in plant pollen extracts. | in an attempt to access the possible role of protease-antiprotease mechanisms of non-immune defence in pollinosis, only low levels of trypsin-, kallikrein- or plasmin-like proteinases could be detected in aqueous pollen extracts. in contrast, several pollen species displayed appreciable trypsin inhibitory activity, e.g. parietaria, olea, ambrosia, rumex, chenopodium, holcus and poa spp. these proteins of the serpin family of anti-proteinases were found to bind specific ige-antibodies from the se ... | 1995 | 7556577 |
| pollen grains bind to lung alveolar type ii cells (a549) via lung surfactant protein a (sp-a). | lung surfactant protein a (sp-a) is the most abundant surfactant-associated protein present in the lung. a receptor for sp-a has been shown to be present on a549 alveolar type ii cells and on other cell types, including alveolar macrophage. the sp-a receptor on a549 cells has been identified as the collection receptor, or c1q receptor, which binds several structurally-related ligands. sp-a contains c-type lectin domains, but the role of carbohydrate binding by sp-a in physiological and pathologi ... | 1993 | 8374060 |
| comparison of gramineae and ragweed pollen counts measured by hirst's and cour's traps. | the aim of this study is to appreciate what conclusions may be drawn from the comparison between two different methods for pollen count determination in clinical allergology. in lyon (france), two pollen collectors are set up in two places separated by ten kilometers, as the crow flies. the hirst-type trap is on the roof of the pasteur institute in the town; the cour's trap (french association for ragweed study), is located at the meteorological station in the country at the east of the town. in ... | 1993 | 8489697 |
| comparison of allergenic potentials of timothy (phleum pratense) pollens from different pollen seasons collected in the belgrade area. | we investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, sds-page, immunoblot, rast, rast inhibition, and crossed immunoelectrophoresis. extract of the pollen from 1991 showed the lowest yield in quantitative assays. sds-page, crossed immunoelectrophoresis, rast, and rast inhibition expressed approximately comparable patterns for all extracts except that from 1991. obviously, the quality of grass pollens, as shown for some ragweed (ambro ... | 1997 | 9105527 |
| structural analysis of n-glycans from allergenic grass, ragweed and tree pollens: core alpha1,3-linked fucose and xylose present in all pollens examined. | the n-glycans from soluble extracts of ten pollens were examined. the pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase hplc, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. in comparison to known structures, it was possible to determine the major structures of the n-glycans derived from kentucky blue grass (poa pratensis), rye (secale cerale), ryegrass (lolium ... | 1998 | 10386890 |
| in vitro allergen-induced degranulation of pulmonary mast cells from horses with recurrent airway obstruction (heaves). | to determine the capacity of pulmonary mast cells (pmc) to degranulate in response to various potential allergens and other secretagogues in horses with recurrent airway obstruction (heaves) and clinically normal horses before and after exposure to moldy hay. | 1999 | 10407477 |
| the free amino groups of ambrosia elatior pollen proteins. | | 1953 | 13052363 |
| [a new possible factor of pollinosis in piemonte; ambrosia elatior]. | | 1954 | 13165248 |
| meteorological variables connected with airborne ragweed pollen in southern hungary. | about 30% of the hungarian population has some type of allergy, 65% of them have pollen sensitivity, and at least 60% of this pollen sensitivity is caused by ragweed. the short (or common) ragweed (ambrosia artemisiifolia = ambrosia elatior) has the most aggressive pollen of all. clinical investigations prove that its allergenic pollen is the main reason for the most massive, most serious and most long-lasting pollinosis. the air in the carpathian basin is the most polluted with ragweed pollen i ... | 2004 | 15103548 |
| hypersensitivity to pollen allergens on the adriatic coast. | this paper describes a study of air concentrations of pollens and a calendar of pollination around the town of split on the croatian adriatic in 1994. high pollen concentrations of parietaria officinalis dominated during the year (up to 20% from april to june) followed by the pollens of pistacia lentiscus, olea europaea, pinus halepensis, juniperus oxycedrus, acacia baileyana, artemisia vulgaris, ambrosia elatior and cistus monspeliensis. in 1994-95, skin prick tests using commercially available ... | 2004 | 15285462 |
| allergic rhinitis and asthma in southern croatia: impact of sensitization to ambrosia elatior. | to identify pollen types in southern croatia and investigate the impact of sensitization to ambrosia elatior (a. elatior) on symptoms and treatment of patients with seasonal allergic rhinitis and/or asthma. | 2007 | 17309141 |
| different phenotypes of intermittent and persistent respiratory allergy in zagreb, croatia. | allergic rhinitis (ar) is a major chronic respiratory disease because of its prevalence, its impacts on the quality of life, economic burden and links with asthma. a significant relationship between the severity of rhinitis and prevalence of asthma in allergic patients was found both in patients suffering from seasonal rhinitis and in those suffering from perennial-allergic rhinitis (par) and asthma. the aim of the study was to investigate allergy phenotypes in patients with intermittent allergi ... | 2009 | 19572486 |
| the distribution of hla alleles among children with atopic asthma in croatia. | allergic asthma is a multifactorial disease involving well known environmental factors and less identified genetic components. in several studies the hla genes have been implicated in the development of asthma and atopy, but the importance of these associations remains unclear. the aim of the present study was to analyse the distribution of specificities at hla class i loci (-a and -b) and hla class ii locus (-drb1) in a group of 143 croatian children with atopic asthma, regarding total serum ig ... | 2011 | 22397267 |