estimation of ige antibodies to the common allergens by reverse enzyme immunoassay. comparison with the radioallergosorbent test.a reverse enzyme immunoassay (reina) is described, in which polystyrene microtiter wells are sensitized with murine monoclonal anti-human ige, and then sequentially allowed to react with patient's serum, peroxidase-labeled allergens and substrate. the results obtained with the sera of patients allergic to lolium perenne grass pollen, the tree pollens of betula alba and olea europea, the epithelia of cat and dog, the mite dermatophagoides pteronyssinus, or to the foodstuffs cow's milk, chicken eg ...19911669584
seasonal asthma caused by airborne platanus pollen.this work describes three patients suffering from bronchial asthma after being naturally exposed to airborne plane-tree pollen. the three patients gave immediate response in skin tests and dual response in bronchial provocations using platanus hybrida extract. there was specific seric ige activity against this/these antigen(s) with the cap system. the three patients also showed significant correlation (p < 0.001) between their rhinitis and asthma symptom-scores registered on their diary cards an ...19947889425
cloning and expression of ole e i, the major allergen from olive tree pollen. polymorphism analysis and tissue specificity.ole e i, the major allergen from the olive tree (olea europaea), is one of the main causes of allergy in mediterranean countries and some areas of north america. the cloning and sequencing of several cdnas coding for the olive allergen have been achieved. cdna has been synthesized from total pollen rna and amplified by using the polymerase chain reaction. the nucleotide sequence data demonstrate the existence of microheterogeneities in at least 37 positions out of the 145 amino acids of ole e i, ...19948195158
olive (olea europea) and privet (ligustrum vulgare) pollen allergens. identification and cross-reactivity with grass pollen proteins.protein blotting studies showed that three olive pollen components with mol. wts approximately 18-19, 20 and 40 kd can be considered to be major allergens. for privet pollen, the highest recognition frequencies were for allergens of mol. wts approximately 20, approximately 19, approximately 40 and approximately 70 kd. when results with the 62 subjects examined were separated into groups corresponding to their geographical locations, viz. italy, france and australia, subjects sensitized to olive, ...19921528191
allergenic pollen pollinosis in madrid.a 15-year pollen count was performed in the atmosphere of madrid, spain, to determine the months in which the highest concentrations of allergenic pollens occur.19957622759
allergy to olea europaea pollen: relationship between skin prick tests, rast, elisa and bronchial provocations tests.olea europaea is the most important allergenic tree in southern italy and in the naples area. its pollination period lasts from the middle of april to the end of june. in our pollinosis patients we observed frequency of sensitization of 13.05% to olea pollen. that means that this pollen follows parietaria (47.80%) and gramineae (34.70%) regarding the frequency of allergic sensitization. in an attempt to evaluate the degree of allergic sensitization to olea pollen and the concordance of various t ...19853898790
acute antihypertensive effect in conscious rats produced by some medicinal plants used in the state of são paulo.thirty two medicinal plants used popularly for their proposed diuretic and/or antihypertensive properties have been tested for antihypertensive effects in conscious unrestrained rats. all extractions were made in aqueous ethanol (50:50 by vol.) at low temperature. before administration of the extracts the alcohol was evaporated. the extracts (40 ml/kg) were always administered per os. antihypertensive effects in shr rats were observed after the administration of allium sativum linn. (bulb), olea ...19863724206
air pollution effects on the leaf structure of two injury resistant species: eucalyptus camaldulensis and olea europaea l. 19911768961
[immediate hypersensitivity to fruits and vegetables and pollenosis].various works have been published, mainly by scandinavian authors, in which a partial immunological identity has been found to exist between birch pollen and hazelnut and apple. however, our attention has been particularly drawn to the high proportion of people who are allergic to the apple and other fruits, which in some cases has been as much as 50 and 70%, and in whom an oral pruritus alone is considered sufficient a symptom of allergies to certain foodstuffs. however due to the fact that the ...19854036764
atmospheric pollen in the area of this paper the results of an investigation on the pollen grains in the atmosphere of athens, greece, are presented. the work was carried out between june 1973 and august 1974. a gravity sampler, of the durham type, was placed on the roof of king paul athens general hospital. slides covered with vaseline were exposed to the air for 24 h every day during the above-mentioned period. after staining , the pollen on each slide was counted in an area of 1.375 cm2 under a light microscope. the result ...1977577084
annual, daily and diurnal variations in pollen from olea europaea l. in the atmosphere of granada (spain).the allergenic capacity of olea europaea l. pollen is very important in several cities of the southern iberian peninsula, producing most pollinoses diagnosed in these cities. we have carried out a study on the annual, daily and diurnal variations in pollen from o. europaea in the atmosphere of granada (spain) during 4 consecutive years. samplings were carried out with the aid of a burkard spore trap. very low levels of olea pollen grains were first detected in the atmosphere from the end of apri ...19938298749
vasodilator effect of olive leaf.we studied the importance of the smooth vascular muscle endothelium in the vasodilator action of the decoction of olive (olea europaea) leaf. the decoction caused relaxation of isolated rat aorta preparations both in the presence (ic50 1.12 +/- 0.33 mg/ml) and in the absence (ic50 1.67 +/- 0.16 mg/ml) of endothelium. the results indicate that the relaxant activity of the lyophilized decoction is independent of the integrity of the vascular endothelium. we also showed that oleuropeoside is a comp ...19911798793
regulation of 3-indoleacetic acid production in pseudomonas syringae pv. savastanoi. purification and properties of tryptophan 2-monooxygenase.the oxidative decarboxylation of l-tryptophan to yield 3-indoleacetamide, catalyzed by tryptophan 2-monooxygenase, represents a controlling reaction in the synthesis of indoleacetic acid by pseudomonas savastanoi (pseudomonas syringae pv. savastanoi), a gall-forming pathogen of olive (olea europea l.) and oleander (nerium oleander l.). production of indoleacetic acid is essential for virulence of the bacterium in its hosts. tryptophan 2-monooxygenase was characterized to determine its role in in ...19853997822
olea europaea airborne pollen in southern spain.olea europaea pollen is one of the most abundant constituent pollens in seville (southern spain). it is responsible for many documented cases of pollinosis in the area.19979087152
multicentre evaluation of capture assay radim liquid allergen for measurement of specific ige antibodies.a multicentre trial of capture assay radim liquid allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. the results of the evaluation were compared with clinical data and in vivo testing. good agreement was obtained for dermatophagoides pteronyssinus (d1), cat's epithelium (e1), betula verrucosa (t3) and olea europea (t9), artemisia vulgaris (w6) and parietaria officinalis (w19). some spreading of data was observed for art ...19958608200
evaluation of serum specific ige and skin responsiveness to allergenic extracts of oleaceae pollens (olea europaea, fraxinus excelsior and ligustrum vulgare) "in patients with respiratory the last few years an increasing interest for olea europaea (o.e.) pollen has been developed in many countries. several authors have studied the aerobiological, allergenic, epidemiological and clinical features of this pollinosis. the aim of our study was to evaluate cutaenous and serological responses to other oleaceae pollens allergens (ligustrum vulgare (l.v.) and fraxinus excelsior (f.e.) in patients with skin positivity to olea europaea pollen extract. twenty-three atopic rhinitis and/or ...19957631595
mercurialis annua: characterization of main allergens and cross-reactivity with other species.a multicentric study was conducted to evaluate the frequency of mercurialis annua pollen sensitization in several areas of spain and to select a population sample to characterize the main allergenic components in m. annua pollen. patients were recruited from six hospitals in spain. out of 420 patients sensitized to pollens, 195 (46.4%) showed positive skin tests to m. annua, thus evidencing the high level of sensitization to the pollen of this plant in spain. thirty-seven sera with rast class va ...19979104791
spring pollen and related allergies in southern spain.over the last 10 years, there has been an increase in pollen allergies throughout europe; this trend is clearly reflected in this study carried out in córdoba (southern spain) over 7 consecutive years, which recorded important increases in the concentrations of certain pollen types of particular interest in this city, together with an increase in the number of patients with pollinosis visiting the allergy unit at córdoba's reina sofía university hospital. the purpose of this study was to ascerta ...19938298753
control of karoo paralysis ticks through vegetation management.karoo paralysis, caused by feeding ixodes rubicundus females, is a major disease of small stock in south africa. control methods currently practised are almost exclusively chemical based. to limit overdependance on chemicals, vegetation management was investigated as a possible method for control, to be incorporated in an integrated tick management system. laboratory and field experiments were conducted to determine, firstly, the extent of vertical migration and survival of ticks on long and sho ...19968834741
allergenic pollens in the southern mediterranean the mediterranean area there are characteristic climatic conditions (mildness of winter, summer dryness, etc.) that facilitate the growing of a typical vegetation with production of allergenic pollen, such as those from parietaria and olea europaea, very different from that of central and northern europe. we present in this article the results of an 8-year pollen count in the atmosphere of naples, italy. the pollen content was examined with a volumetric spore trap (lanzoni vpps-2000) like tha ...19892913131
month of birth, atopic disease, and sensitization to common aeroallergens in greece.the aim of the study was to evaluate the influence of the month of birth on the development of atopic disease and the rate of sensitization to common environmental allergens in greece. analysis of the month of birth of 1755 individuals with atopic disease (asthma, rhinitis, atopic dermatitis), out of whom 771 had positive skin tests/rast to dermatophagoides pteronyssinus and mixed grasses, in comparison to total live births (2,877,733) in greece during 1968-88, was performed and showed significa ...19958822396
characterization of recombinant mercurialis annua major allergen mer a 1 (profilin).two major allergens (mer a 1a and mer a 1b), tentatively identified as profilin, have been described in the euphorbiacea, mercurialis annua.19989525453
respiratory allergy to parietaria pollen in 348 subjects.we have revisited 348 records of allergic outpatients to parietaria, ratio m/f 1:1 and mean age 29.46 +/- 12.49 yrs. 97 of them were skin positive to parietaria only (a-group: m/f 37/60); 251 patients were skin positive also to other allergens (b-group: m/f 137/114). mean age of a-group patients (35.41 +/- 12.91) was higher than b-group (27.16 +/- 11.54; p < 001). dividing mono and polysensitized subjects by age decade, a different distribution was found between the two groups (p < 001): monosen ...19968882753
hypersensitivity to pollen allergens on the adriatic central south croatia, i.e., the adriatic coast with the city of split at the center, year-long pollen concentrations in the air were determined for typical local plants, and the area's plant pollination calendar was established. high concentrations of parietaria officinalis pollen dominated during the year (up to 20% from april to june). pollens of pistacia lentiscus, olea europaea, pinus halepensis, juniperus oxycedrus, mimosa and cistus monspeliensis were found in lower concentrations and ...19947921334
pollinosis in a mediterranean area (riviera ligure, italy): ten years of pollen counts, correlation with clinical sensitization and meteorological data.we present in this study the results of 10 years of pollen counts in the atmosphere of san remo, italy. these results were compared with clinical sensitizations in 5481 pollen-allergic patients of both sexes (children and adults) living in the same area. pollen counts showed that the pollens found in greatest quantities were the cupressaceae (22.4%), followed by the urticaceae (parietaria officinalis, 17.4%), the oleaceae (olea europaea, 10.8%) and the pinaceae (10.1%), the poaceae being present ...19947921331
pollen-related allergy in italy.pollen-related allergies are very common in italy and pollinosis is the commonest allergic disease. the type of allergenic plants and the prevalence of hay fever varies among regions. in the mediterranean area there are characteristic climatic conditions (mildness of winter, summer dryness) that facilitate the growth of a typical vegetation with its associated various types of allergenic pollen grains, some of them very different from those of central and northern europe. italy has a central pos ...19921586007
apparent digestibility of dietary fibre and other components in table olives.the apparent digestibility of the main components of the olive (olea europaea arolensis) has been studied using young wistar rats. an appreciable digestibility of fibre was found (of the order of 31% neutral detergent fibre) and a high digestibility of fats (higher than 60%) and sugars (100% of glucose and 76% of fructose). some 41% of the protein was digested, a relatively low value when compared with other products. if the diet is previously desalted, the digestibility of the components dimini ...19938395658
comparative studies of allergenic extracts prepared from freshly collected, dried, or dried and defatted pollen.fresh pollen samples of parietaria judaica, betula pubescens, dactylis glomerata and olea europaea were collected and aliquot portions of these pollens were stored after drying in hot air, while other portions were dried and then defatted with diethylether. the different pollen materials were extracted with aqueous media to obtain the non-dialyzable allergenic protein constituents. review of the production data, together with comparative analyses of the uv-absorption spectra and of ige binding p ...19957582159
survey of the allergic status of patients with bronchial asthma in turkey: a multicenter study.patients diagnosed with bronchial asthma (ba) were prospectively enrolled to assess their allergen spectra and atopic status. the patients came from five major cities (ankara, izmir, samsun, elaziğ, and adana) in different regions of turkey. atopic status, total ige levels, and allergen spectra were determined in 1149 patients and 210 controls who were spouses of the patients sharing the same environment but not consanguinity with the patients. total ige levels were significantly higher in the a ...19957573836
prevalence of atopy in students from málaga, spain.epidemiologic studies are necessary to determine the prevalence of allergic diseases. this varies widely depending on allergen preparations and patients studied.19989532972
prevalence of patent and latent atopy among a general normal adult population in the south east of france by rast investigation and correlation with circulating total ige levels.a population of 512 adults (men: 266, 52%, women: 246, 48%) with mean age 41.4 years (s.d. 10.7) was investigated. this population was subdivided into five groups: marseilles city, manosque a small town in a prealpine area, the area surrounding the etang de berre, a rural area in northern bouches du rhône and chateaurenard, a small town in an intensive agricultural area. rasts to a panel of eight allergens (lolium perenne, parietaria officinalis, cupressus sempervirens, olea europea, cat dander, ...19938160568
immunogold probes for light and electron microscopic localization of ole e i in several oleaceae pollens.we investigated the immunolocalization of the olive major allergen ole e i and ole e i-like proteins in pollen from several oleaceae species [olive (olea europaea), ash (fraxinus excelsior), privet (ligustrum vulgaris), lilac (syringa vulgare), and forsythia (forsythia suspensa)]. crossreactions among different pollens were found in enzyme immunoassays. for immunolocalization with light microscopy we used the silver enhancement technique with three monoclonal antibodies (1d8, 10h1, and 16g2) tha ...19968609371
recombinant dna technology in allergology: cloning and expression of plant profilins.profilin, an ubiquitous protein involved in eukaryotic cytoskeleton regulation, has been previously described as allergen in grasses, weeds and trees and in many fruits and vegetables, and it is in part responsible for cross-reactivities pollen and food allergic patients. completed cdna clones coding for phleum pratense, olea europaea, cynodon dactylon, parietaria judaica and helianthus annuus pollen profilins were isolated and sequenced. the deduced amino acid sequences share high identity with ...19979208049
structural analysis of n-glycans from allergenic grass, ragweed and tree pollens: core alpha1,3-linked fucose and xylose present in all pollens examined.the n-glycans from soluble extracts of ten pollens were examined. the pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase hplc, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. in comparison to known structures, it was possible to determine the major structures of the n-glycans derived from kentucky blue grass (poa pratensis), rye (secale cerale), ryegrass (lolium ...199810386890
production and characterization of profilin monoclonal antibodies.profilins have been identified as a pan-allergen of different plant pollens and foods. in this paper, we describe the generation of monoclonal antibodies (mabs) by immunizing balb/c mice with helianthus annuus purified profilin in order to characterize this important and common allergen. a panel of forty mabs has been obtained, and twenty of them were used to map antigenic determinants in this molecule. at least two different antigenic determinants were recognized in h. annuus profilin by immuno ...19979208051
evaluation of the potency of allergenic extracts by inhibition of igg-antibody binding.a method is described for the in vitro potency evaluation of allergenic extracts by using their capacity of binding to specific human igg antibodies. the results obtained with this inhibition-type enzyme immunoassay are compared with the analyses by the customary method of ige antibody binding. a large series of allergenic protein fractions from the pollen of the gramineae, olea europea, parietaria judaica, and from the dust mite dermatophagoides pteronyssinus as well as from cat dander and pean ...19947917232
[hypotension, hypoglycemia and hypouricemia recorded after repeated administration of aqueous leaf extract of olea europaea l].this study is concerned by the determination of the toxicity of an olea europaea l.aqueous leaf extract after repeated administrations. female and male rats were given 37.5, 75, 150, 300, 600 and 1200 mg/kg/24h of the extract for 60 days. the results show that the drug studied induces an increase of weight growth, an hypotension, an hypoglycaemia and an hypouricaemia in treated animals.19948035301
determination of ole e 1 by enzyme immunoassay and scanning densitometry. validation by skin-prick testing.ole e 1 is an important allergen in olea europaea pollen extracts. this study describes the development of two new methods that can be used to estimate the ole e 1 content in olive tree pollen extracts. they are based on (1) an enzyme immunoassay that uses rabbit polyclonal, monospecific antibodies and purified ole e 1, and (2) scanning densitometry of sds-page gels. twelve extracts were evaluated by in vivo and in vitro methods. the in vivo biological potency was estimated by prick skin testing ...199910037231
sensitization to olea europaea: geographical differences and discrepancies.thirty eight patients from two geographical areas of spain, with great differences in olea europaea pollen counts were studied to investigate their in vivo and in vitro immune response to this pollen as a consequence of their different environmental allergen exposure. they were distributed in two groups (13 from madrid, and 25 from jaén). skin sensitivity was assessed by a prick-test dose-response bioassay using serial dilutions of a biologically standardized allergen extract of o. europaea. ser ...19979269505
temporal and spatial gene expression of cytochrome b5 during flower and fruit development in olives.we report the characterisation of two cytochrome b5 genes and their spatial and temporal patterns of expression during development in olive, olea europaea. a pcr-generated probe, based on a tobacco cytochrome b5 sequence, was used to isolate two full-length cdna clones (cytochrome b5-15 and cytochrome b5-38) from a library derived from 13 waf olive fruits. the cdnas encoded proteins of 17.0 and 17.7 kda, which contained all the characteristic motifs of cytochromes b5 from other organisms and exh ...199910394947
two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases.two new triterpene synthase cdnas, named as oew and trw, were cloned from olive leaves (olea europaea) and from dandelion roots (taraxacum officinale), respectively, by the pcr method with primers designed from the conserved sequences found in the known oxidosqualene cyclases. their orfs consisted of 2274 bp nucleotides and coded for 758 amino acid long polypeptides. they shared high sequence identity (78%) to each other, while they showed only about 60% identities to the known triterpene syntha ...199910542078
cloning and expression of the panallergen profilin and the major allergen (ole e 1) from olive tree pollen.olive tree (olea europaea) pollen allergy is one of the main causes of allergy in mediterranean countries and some areas of north america.19979314349
cross-reactivity of olea europaea with other oleaceae species in allergic rhinitis and bronchial asthma.cross-reactivity between pollen extracts of four species of oleaceae was studied: olive (olea europaea), ash (fraxinus excelsior), privet (ligustrum vulgare), and lilac (syringa vulgaris). thus, 51 patients and 13 atopic controls were studied, by means of intracutaneous skin tests, histamine-release tests against the four extracts, and specific ige to o. europaea. the proteic content of the four extracts was assessed by sds-page and immunoblotting, and similarity of all the extracts studied was ...19979284982
particularities of pollen allergies in greece.pollen allergy is a significant clinical problem in older greek children causing approximately 25% of respiratory allergic disease in children of mean age 10 years. this is due to climatic conditions that favor rich and long pollinic seasons more so in central and southern greece as well as the greek islands. similarities are evident between greece and other mediterranean countries, especially southern italy and spain. most prevalent allergenic plants with known clinical significance are grasses ...199910093133
[human basophil degranulation test. results of a modified technic (i)].the degranulation of human basophils towards antigenic substances, to which they must be previously sensitized as a result of the antigen-antibody union at the membrane level, is a phenomenon that has an important application complementing diagnosis of diseases mediated by the gell and coombs classification of type i hypersensitivity, on which the principle of human basophils degranulation test (hbdt) was based. an essential requirement is to be able to collect an adequate quantity of basophils, ...19863776778
spatial distribution of the karoo paralysis tick ixodes rubicundus (acari: ixodidae) within a false upper karoo veld type.spatial distribution of the karoo paralysis tick (ixodes rubicundus) has been studied in a paralysis enzootic area of the southwestern orange free state. significantly more females than males were collected from the vegetation. most ticks (99%) used grass as questing sites at a height of approximately 45 cm (range 10-97 cm), which correlates with the size of host animals. no significant differences between the questing height of male and female ticks were evident. the ticks were found on most of ...19911860416
molecular characterization of a cross-reactive juniperus oxycedrus pollen allergen, jun o 2: a novel calcium-binding allergen.species belonging to the cupressaceae family are a relevant source of allergens that are present in a wide number of countries.19989648704
specific ige to recombinant vegetal panallergen (rbet v 2) and fruit allergy in pollinic patients.the presence of ige antibodies to the allergens rbet v 1 and rbet v 2 was investigated in patients with known sensitization to ryegrass (lolium perenne) and/or olive (olea europaea) pollen, by comparing a group of 10 patients who had allergic symptoms after ingestion of fruits (a) with a group of 17 patients who had only seasonal respiratory symptoms (b). there was no significant difference between the two groups for total ige. all patients showed specific ige to both l. perenne and o. europaea. ...19989689344
evaluation of the overall degree of sensitization to airborne allergens by a single serologic test: implications for epidemiologic studies of most epidemiologic studies of respiratory allergy, the overall degree of sensitization is usually measured by indices based on skin prick test reactions to a panel of relevant airborne allergens. in the present study we used a single assay (cap-phadiatop, pharmacia, uppsala, sweden), which is based on the reaction between serum-specific ige and a mixture of locally relevant inhalant allergens to measure the degree of sensitization to inhalants in the sera of 1815 young italian men, in whom a ...19948308184
virgin olive oil volatile compounds from lipoxygenase pathway and characterization of some italian cultivars.fruits from seven different varieties of olea europaea l., grown in the same environmental conditions, were harvested in two succeeding years at the same ripening degree and immediately processed. the oils obtained were submitted to gas chromatographic determination of the volatile compounds extracted by dynamic headspace technique. the results demonstrated that the accumulation of the different metabolites in the oils obtained from the various cultivars were strictly connected with the varietal ...199910552376
polyphenolic content in five tuscany cultivars of olea europaea this study polyphenolic compounds extracted from olive fruits of five registered cultivars were analyzed. a solid-liquid extraction (lse) procedure with extrelut cartridge (diatomaceous earth) using different eluents was developed to obtain polyphenolic compounds. hplc-dad and hplc-ms methods were applied for the quali-quantitative analysis of each fraction obtained from lse. the results of this work show that the lse procedure with diatomaceous earth cartridge supplies a rapid and reproducib ...199910552399
application of stable isotope ratio analysis to the characterization of the geographical origin of olive gain information about the geographical origin of oil samples, measurements of delta(13)c and delta(18)o of the whole oil and some of its fractions have been performed on samples coming from fruits of olea europaea l. produced in greece, morocco, spain, italy, tunisia, and turkey. the results obtained by applying statistical procedures have given pieces of evidence that oil samples have shown the trend to cluster according to the different climatic areas of growing environment of fruits. some ...199910552408
allergenic cross-reactivity of olive pollen.sera of patients allergic to olive (olea europaea) pollen were used to analyze the ige cross-reactivity between olive-pollen extract and other pollens obtained from phylogenetically unrelated species.200010921466
in vitro antibacterial activity of some aliphatic aldehydes from olea europaea the present paper we report the 'in vitro' activity of eight aliphatic long-chain aldehydes from olive flavor (hexanal, nonanal, (e)-2-hexenal, (e)-2-eptenal, (e)-2-octenal, (e)-2-nonenal, (e)-2-decenal and (e,e)-2,4-decadienal) against a number of standard and freshly isolated bacterial strains that may be causal agents of human intestinal and respiratory tract infections. the saturated aldehydes characterized in the present study do not exhibit significant antibacterial activity, while the ...200111325546
the role of temperature in the onset of the olea europaea l. pollen season in southwestern spain.temperature is one of the main factors affecting the flowering of mediterranean trees. in the case of olea europaea l., a low-temperature period prior to bud development is essential to interrupt dormancy. after that, and once a base temperature is reached, the plant accumulates heat until flowering starts. different methods of obtaining the best-forecast model for the onset date of the o. europaea pollen season, using temperature as the predictive parameter, are proposed in this paper. an 18-ye ...200111411416
on the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol.secoiridoides (oleuropein and derivatives), one of the major classes of polyphenol contained in olives and olive oil, have recently been shown to inhibit or delay the rate of growth of a range of bacteria and microfungi but there are no data in the literature concerning the possible employment of these secoiridoides as antimicrobial agents against pathogenic bacteria in man. in this study five atcc standard bacterial strains (haemophilus influenzae atcc 9006, moraxella catarrhalis atcc 8176, sal ...199910504039
the colonization history of olea europaea l. in macaronesia based on internal transcribed spacer 1 (its-1) sequences, randomly amplified polymorphic dnas (rapd), and intersimple sequence repeats (issr).phylogenetic relationships in the olea europaea complex and the phylogeography of 24 populations of the macaronesian olive (o. europaea ssp. cerasiformis) were assessed by using three molecular markers: nuclear ribosomal internal transcribed spacer 1 (its-1) sequences, randomly amplified polymorphic dnas (rapd), and intersimple sequence repeats (issr). parsimony analysis of the its-1 sequences and neighbour-joining (nj) analyses of rapd and issr banding variation revealed four major lineages in ...200010886649
volatile compounds from three cultivars of olea europaea from italy.the chemical compositions of the volatile fractions from three olea europaea l. cultivars (leccino, frantoio, and cipressino) were examined by gc and gc-ms. the results showed that the cultivars can be distinguished on the basis of the volatile fraction compositions.200111714335
encoding of host and non-host plant odours by receptor neurones in the eucalyptus woodborer, phoracantha semipunctata (coleoptera: cerambycidae).in the eucalyptus woodborer, phoracantha semipunctata, responses from single receptor neurones to host ( eucalyptus globulus) and non-host ( pinus pinaster, olea europeae) plant odours were examined, using gas chromatography linked with electrophysiological recordings. thirty-two compounds elicited responses that appeared as increased firing rates correlating with the elution of the active components. a subset of neurones classified as type a ( n=17) responded to one compound, a second subset b ...200211919693
blood pressure lowering effect of an olive leaf extract (olea europaea) in l-name induced hypertension in rats.a specially prepared olive leaf extract (efla 943) has been tested for its blood pressure lowering activity in rats rendered hypertensive by daily oral doses of l-name (ng-nitro-l-arginine methyl ester, 50 mg/kg) for at least 4 weeks. oral administration of the extract at different dose levels at the same time as l-name for a period of 8 weeks showed a dose dependent prophylactic effect against the rise in blood pressure induced by l-name, best effects being induced by a dose of 100 mg/kg of the ...200212489249
[therapeutic effect of olea europea var. oleaster leaves on carbohydrate and lipid metabolism in obese and prediabetic sand rats (psammomys obesus)].sand rats develop obesity, insulin-resistance, hyperlipidemia and prediabetes, when given a standard laboratory chow diet. we have used this model to demonstrate the beneficial action of olea europea var. oleaster leaves to regulate unbalanced metabolism. 32 sand rats fed on hypercaloric diet during 7 months, were divided into 3 groups: controls (n=10), treated by plant (n=13) and treated by simvastatin (zoco); hypocholesterolemic drug. the plant decoction prepared at 10% was given orally at the ...200010915976
monoclonal antibody-based method for measuring olive pollen major allergen ole e 1.olive tree pollen is an important cause of inhalant allergy in mediterranean countries. the major allergen of this pollen, ole e 1, has caused reactions in the sera of >80% of olive-sensitive patients. accurate standardization of allergenic products for diagnosis and immunotherapy is essential to guarantee their quality, and measurement of the major allergen content is becoming an important aspect of standardization procedures.200212141727
an allergen from olea europaea pollen (ole e 7) is associated with plant-derived food anaphylaxis.several cross-reacting proteins have been identified as responsible of the co-occurrence of pollinosis and plant-derived food allergy. this association has been mainly described in the birch-apple syndrome but other pollens such as olea europaea and other fruits may also contain homologous proteins.200212173271
bioactive derivatives from oleuropein by a biotransformation on olea europaea leaf extracts.a very simple method is proposed to produce, using non-homogeneous hyperthermophilic beta-glycosidase immobilised on chitosan, 3,4-dihydroxy-phenylethanol (hydroxytyrosol), a commercially unavailable compound with well known biological properties which justify a potential commercial application. leaf extracts from olea europaea with high oleuropein content are selected as substrate for biotransformation. under the biotransformation conditions, high amounts of hydroxytyrosol are collected within ...200211738718
radioprotective effects in vivo of phenolics extracted from olea europaea l. leaves against x-ray-induced chromosomal damage: comparative study versus several flavonoids and sulfur-containing compounds.the radioprotective effects of a polyphenolic extract of olea europaea l. leaves (ol); the flavonoids diosmin and rutin, which are widely used as pharmaceuticals; and the sulfur-containing compounds dimethylsulfoxide (dmso) and 6-n-propyl-2-thiouracil (ptu) were determined by using the micronucleus test for anticlastogenic activity, evaluating the reduction of the frequency of micronucleated polychromatic erythrocytes (mnpces) in bone marrow of mouse before and after x-ray irradiation. with trea ...200212495584
pollen calendar of the city of salamanca (spain). aeropalynological analysis for 1981-1982 and 1991-1992.we report a study on the contents of airborne pollen in the city of salamanca (spain) aimed at establishing a pollen calendar for the city for the yearly periods of maximum concentrations, relating these with quantifiable atmospheric variables over two two-year periods with an interval of 10 years between them: 1981-82 and 1991-92. the pollen was captured with burkard spore-traps, based on hirst's volumetric method. determinations were made daily and were used to make preparations, previously st ...19989885728
characteristics of pollinosis caused by betula in patients from ourense (galicia, spain).a retrospective study was performed to describe the features of the pollinosis caused by betula in the area of ourense, spain. the pollen count was carried out with a lanzoni volumetric hirts spore trap (1993-2000). the betula pollen represented 5% over the annual total (annual mean quantity: 965 grains). it was present in the air from march to mid-may. the highest peaks took place in april (maximum values mean: 131 grains/m3). the medical records of 222 patients (mean age 25.66 years) diagnosed ...200312968397
anaphylactic reaction to ingestion of quercus ilex acorn nut.a patient experienced an anaphylactic reaction after eating acorn nuts, fruit of the holm oak (quercus ilex), one of the most abundant trees in spain. several urticaria episodes upon ingestion of peanuts were also referred.19989677139
effect of prolonged vegetative reproduction of olive tree cultivars (olea europaea l.) in mitochondrial homoplasmy and heteroplasmy.the inheritance of mitochondrial and chloroplast genomes does not follow mendelian laws, but proceeds by vegetative segregation. most organisms show organelle homoplasmy, which is probably produced and maintained during sexual reproduction. we have tested the effect of prolonged vegetative multiplication in the maintenance of mitochondrial homoplasmy and the generation of heteroplasmy in cultivated olive trees, olea europaea l. seven trees, each representing a different variety of olive, were an ...200312834053
asthma induced by canary food mix.a 42-year-old woman reported immediate rhinoconjunctivitis, asthma, and contact urticaria while handling bird food. skin-prick tests were positive to lolium, cynodon, phragmites, cupressus sempervirens, cupressus arizonica, chenopodium, sunflower pollen and seed, mugwort, chamomile, chrysanthemum, taraxacum, canary seed, and black seed (guizotia abyssinica). the patient's serum-specific immunoglobulin (ige) to taraxacum, black seed, and canary seed was positive. enzyme-linked immunosorbent assay ...200312974193
radiation-use efficiency and dry matter partitioning of a young olive (olea europaea) orchard.radiation-use efficiency (rue) relates biomass production to the photosynthetically active radiation (par) intercepted by a plant or crop. we determined rue and biomass partitioning coefficients of young olive (olea europaea) trees for use in a general growth model. in 1995, 1-year-old olive trees var. 'picual' were planted at a density of either 0.5 or 2.0 trees m(-2) near córdoba, spain, at a site providing favorable growth conditions. during the experiment (1995-1997), both par interception b ...200012651528
effect of freeze dried extract of olea europaea on the pituitary-thyroid axis in rats.the effect of an aqueous extract of olive (olea europaea) leaf on the thyroid activity was studied. the results suggest a stimulatory action of the extract on the thyroid, unrelated to the pituitary.200212164280
a major allergen from pollen defines a novel family of plant proteins and shows intra- and interspecies [correction of interspecie] cross-reactivity.olive tree (olea europaea) pollen is a main cause of allergy associated with extensive areas of europe and north america. ole e 10, a small (10.8 kda) and acidic (pi 5.8) protein, has been identified as a major allergen from the olive pollen, isolated, and characterized. circular dichroism analysis gave 17% alpha helix, 33% beta sheet, and 21% beta turn for its secondary structure. based on amino acid sequences of tryptic peptides, the protein was cloned and sequenced. the allergen consists of a ...200415004167
identification of microsatellite loci in olive (olea europaea) and their characaterization in italian and iberian olive trees. 200010964237
daily variation patterns of airborne allergenic pollen in southwestern spain.the study was carried out using a burkard sampler installed on the roof terrace of the school of pharmacy, seville, for two years (1995 and 1996). eight pollen types described in the literature as having allergenic activity were chosen. they were poaceae, olea europaea, chenopodiaceae/amaranthaceae, plantago, rumex, urticaceae (including parietaria), cupressaceae, and platanus hispanica. the types were grouped according to the similarity of their pattern of intradiurnal variation in pollen conce ...19989615301
antioxidant activity of maslinic acid, a triterpene derivative obtained from olea europaea.we investigated the effect of maslinic acid (a triterpene derivative obtained from olive pomace), on the susceptibility of plasma or hepatocyte membranes to lipid peroxidation (lpo), induced respectively by the hydroxyl radical (oh*) generated by fe2+/h2o2 ex vivo and by the system fe3+/ascorbate in vitro; moreover, three groups of animals used in the plasma study were pretreated with ccl4 (to generate ccl3-*). endogenous plasma lipoperoxide levels and susceptibility to lpo were decreased in rat ...200312802735
solid-phase microextraction in the analysis of virgin olive oil volatile fraction: characterization of virgin olive oils from two distinct geographical areas of northern italy.spme was employed to characterize the volatile profile of virgin olive oils produced in two geographical areas of northern italy: the region of the gulf of trieste and the area near lake garda. there are as yet no data on the headspace composition of virgin olive oils from these regions, characterized by particular conditions of growth for olea europaea. using the spme technique coupled to gc-ms and gc-fid, the volatile components of 42 industrially produced virgin olive oil samples were identif ...200314558780
allergenicity and cross-reactivity of russian olive pollen (eleagnus angustifolia).the purposes of this study were: to determine the prevalence of sensitization and immunochemical characterization of eleagnus angustifolia pollen (russian olive) that belongs to the family eleagnaceae.200415461599
allergenicity and immunochemical characterization of six varieties of olea europaea.the inhalation of olea europaea pollen is one of the most important causes of allergic respiratory diseases in the mediterranean basin. the objective of this study was to investigate the antigenic and allergenic composition of six different o. europaea varieties collected in southern spain.200211906361
the main allergen of olea europaea (ole e i) is also present in other species of the oleaceae family.three major pollen allergens from fraxinus excelsior, ligustrum vulgare and syringa vulgaris belonging to the oleaceae family were purified. monoclonal antibodies previously raised against the main allergen of olea europaea (ole e i) were used for their purification by affinity chromatography. the three new purified allergens were able to bind human ige from serum of olive-allergic patients in a way analogous to ole e i. crossed radioimmunoelectrophoresis of the four allergens, using anti-olive ...19938319129
production and detailed characterization of biologically active olive pollen allergen ole e 1 secreted by the yeast pichia pastoris.the glycoprotein ole e 1 is a significant aeroallergen from the olive tree (olea europaea) pollen, with great clinical relevance in the mediterranean area. to produce a biologically active form of recombinant ole e 1, heterologous expression in the methylotrophic yeast pichia pastoris was carried out. a cdna encoding ole e 1, fused to a saccharomyces cerevisiae alpha-mating factor prepropeptide using the ppic9 vector, was inserted into the yeast genome under the control of the aox1 promoter. aft ...199910215867
transport of airborne pollen into the city of thessaloniki: the effects of wind direction, speed and persistence.we examined the effect of the wind vector analyzed into its three components (direction, speed and persistence), on the circulation of pollen from different plant taxa prominent in the thessaloniki area for a 4-year period (1996-1999). these plant taxa were ambrosia spp., artemisia spp., chenopodiaceae, corylus spp., cupressaceae, olea europaea, pinaceae, platanus spp., poaceae, populus spp., quercus spp., and urticaceae. airborne pollen of cupressaceae, urticaceae, quercus spp. and o. europaea ...200515503184
genetic diversity and relationships in olive ( olea europaea l.) germplasm collections as determined by randomly amplified polymorphic dna.genetic diversity studies using the rapd technique were carried out in a set of 103 olive cultivars from the world germplasm bank of the centro de investigación y formación agraria (cifa) "alameda del obispo" in cordoba (spain). a total of 126 polymorphisms (6.0 polymorphic markers per primer) out of 135 reproducible products (6.4 fragments per primer) were obtained from the 21 primers used. the number of bands per primer ranged from 4 to 11, whereas the number of polymorphic bands ranged from 3 ...200212582515
cloning and expression of the olea europaea allergen ole e 5, the pollen cu/zn superoxide dismutase.recombinant dna technology does provide pure, well-defined and reproducible products to be used for clinical purposes, by cloning and expressing the cdna of allergens present in a specific extract. ole e 5 is a pollen allergen of olea europaea with an ige-binding frequency of about 35%, which has been identified as a superoxide dismutase (sod). the aim of this study was to clone the cdna of ole e 5, to express ole e 5 in escherichia coli and to characterize its immunoreactivity.200515785077
prospective safety study of immunotherapy administered in a cluster schedule.cluster immunotherapy is becoming an alternative to conventional it due to its shorter schedule, but the safety of such schedule is still controversial. at present, only few studies assess the risk of immunotherapy in a prospective manner, in well-controlled patients, using the same extract and intending to evaluate a single schedule. the aim of the present study is to evaluate the safety of a cluster immunotherapy administration regimen. a total of 91 outpatients (41 male and 50 female), with a ...200415736717
bread eating induced oral angioedema due to alpha-amylase allergy.inhalation of dust from different enzymes can be the cause of occupational asthma in exposed workers. enzymes from different sources are being increasingly used in food. few cases of food allergy to alpha-amylase induced by eating bread have been reported. those cases were reported in bakery-related patients and in a pharmaceutical-industry worker. a 25-year-old farmer suffered sneezing, rhinorrhea, oropharyngeal itching, hoarseness, cough, and non-wheezy dyspnea after eating white bread. skin p ...200415736722
hydrolyzed olive vegetation water in mice has anti-inflammatory activity.fruit and vegetable simple and polyphenols are potent antioxidants. one of the most effective in terms of free radical scavenging is 3,4-dihydroxyphenyl ethanol or hydroxytyrosol (ht), a simple phenol found predominantly in olea europea, or the olive plant. ht is most abundant in the aqueous fraction of olive pulp with trace amounts in the olive oil fraction and in the leaves. for these experiments, we evaluated the anti-inflammatory activity of olive vegetation water (ovw), which we showed prev ...200515930455
effects of drought on photosynthesis in mediterranean plants grown under enhanced uv-b radiation.the effects of drought on the photosynthetic characteristics of three mediterranean plants (olive, olea europea l.; rosemary, rosmarinus officinalis l.; lavender, lavandula stoechas l.) exposed to elevated uv-b irradiation in a glasshouse were investigated over a period of weeks. drought conditions were imposed on 2-year-old plants by withholding water. during the onset of water stress, analyses of the response of net carbon assimilation of leaves to their intercellular co2 concentration were us ...200010937707
erwinia toletana sp. nov., associated with pseudomonas savastanoi-induced tree knots.gram-negative bacteria were isolated from knots induced by pseudomonas savastanoi in olive trees (olea europaea l.). a total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. biochemical characterization indicated that all the strains belong to the family enterobacteriaceae. phylogenetic analyses of the 16s rrna genes of these novel isolates revealed that they formed a homogeneous cluster within erwinia species. dna signatures of these isolates were iden ...200415545461
production of highly purified hydroxytyrosol from olea europaea leaf extract biotransformed by hyperthermophilic beta-glycosidase.a large amount of highly purified hydroxytyrosol (91-94% in weight) is obtained in short time by a simple biotransformation of olea europaea leaf extract by a partially purified hyperthermophilic beta-glycosidase immobilized on chitosan support. the biotransformation conditions have been modulated for increasing the hydroxytyrosol yield, whilst chitosan and chitin matrices are used as adsorbent materials in liquid phase hydroxytyrosol extraction from the biotransformed mixtures. natural and non- ...200415196771
hla class ii genotypic frequencies in atopic asthma: association of drb1*01-dqb1*0501 genotype with artemisia vulgaris allergic asthma.different human leukocyte antigen (hla) class ii alleles have been associated with the development of atopic asthma. to determine whether hla class ii alleles are associated with atopic asthma in a population from southeast spain (murcia region), 213 atopic asthmatic patients and 150 controls were selected for hla typing. significant association of the drb1*01 and dqb1*0501 alleles was found in artemisia vulgaris allergic patients (p(c) = 0.00052 and p(c) = 0.00023, respectively). no significant ...200312878360
model for forecasting olea europaea l. airborne pollen in south-west andalusia, on predicted average and maximum airborne pollen concentrations and the dates on which these maximum values are expected are of undoubted value to allergists and allergy sufferers, as well as to agronomists. this paper reports on the development of predictive models for calculating total annual pollen output, on the basis of pollen and weather data compiled over the last 19 years (1982-2000) for córdoba (spain). models were tested in order to predict the 2000 pollen season; in addition, and ...200111513048
the c-terminal segment of the 1,3-beta-glucanase ole e 9 from olive (olea europaea) pollen is an independent domain with allergenic activity: expression in pichia pastoris and characterization.several allergenic proteins, such as the 1,3-beta-glucanases, have been associated with plant defence responses. ole e 9 (46 kda) is a 1,3-beta-glucanase and major allergen from olive pollen, which is a principal cause of allergy in mediterranean countries. its c-terminal segment (101 amino acid residues) has been produced as a recombinant polypeptide in the yeast pichia pastoris. the cdna encoding the polypeptide was inserted into the plasmid vector ppiczalpha-a and overexpressed in km71 yeast ...200312392450
[legume cross-reactivity].legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ...200312783766
volatiles from leaves, fruits, and virgin oil from olea europaea cv. olivastra seggianese from italy.the volatiles produced by leaves and fruits of olea europaea cv. olivastra seggianese have been analyzed in two different phenological stages. furthermore, the volatiles of the virgin olive oil obtained from ripe fruits has been characterized. the volatiles were sampled by means of two different techniques: hydrodistillation and spme. differences were observed between the two different collection times, the different organs, and sampling techniques. the major constituents were often aldehydes, p ...200312590485
evaluation of anthelmintic properties of some plants used as livestock dewormers against haemonchus contortus infections in sheep.gastrointestinal helminth infections remain a major constraint to livestock production globally. this study evaluated anthelmintic efficacy of 7 plants used as dewormers by farmers and pastoralists in kenya. thus 3 commercial anthelmintics and 7 plant preparations were tested in lambs infected with 5000 or 3000 l3 haemonchus contortus in 4 experiments. in the first experiment, ivermectin, levamisole and albendazole were tested in 46 lambs. seven plant preparations of hagenia abyssinica, olea eur ...200415376783
relative performance of european grapevine moth (lobesia botrana) on grapes and other hosts.the european grapevine moth, lobesia botrana is a major grapevine pest, but despite the abundance of vineyards it is a generalist and uses either grapes or alternative species. given the abundance and predictability of grape, l. botrana could be expected to have evolved towards monophagy. in order to understand why this species remains polyphagous, we hypothesized that larvae reared on rare wild host plants should have higher fitness than those reared on the more abundant grape host. for this, w ...200515791428
the olive leaf extract exhibits antiviral activity against viral haemorrhagic septicaemia rhabdovirus (vhsv).a commercial plant extract derived from olive tree leaf (olea europaea) (lext) and its major compound, oleuropein (ole), inhibited the in vitro infectivity of the viral haemorrhagic septicaemia virus (vhsv), a salmonid rhabdovirus. incubation of virus with lext or ole before infection reduced the viral infectivity to 10 and 30%, respectively. furthermore, lext drastically decreased vhsv titers and viral protein accumulation (virucidal effect) in a dose dependent manner when added to cell monolay ...200515869811
hypersensitivity to pollen allergens on the adriatic coast.this paper describes a study of air concentrations of pollens and a calendar of pollination around the town of split on the croatian adriatic in 1994. high pollen concentrations of parietaria officinalis dominated during the year (up to 20% from april to june) followed by the pollens of pistacia lentiscus, olea europaea, pinus halepensis, juniperus oxycedrus, acacia baileyana, artemisia vulgaris, ambrosia elatior and cistus monspeliensis. in 1994-95, skin prick tests using commercially available ...200415285462
differential response of delta13c and water use efficiency to arbuscular mycorrhizal infection in two aridland woody plant species.during a revegetation field experiment in southeast spain, we measured foliar carbon isotope ratios (delta13c) and gas exchange parameters in order to evaluate the influence of arbuscular mycorrhizal (am) infection on the water use efficiency (wue) of two semiarid woodland species. wue during drought was significantly enhanced by inoculation with glomus intraradices in olea europaea ssp sylvestris, but not in rhamnus lycioides. while olea is a long-lived, slow-growing evergreen tree with a conse ...200316228249
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