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[changes in dna methylation in alfalfa plants infected with cuscuta and tissue differences in dna methylation of the parasite plants].the tissue-specific differences in the 5-methylcytosine (m5c) content in total dna of the parasite plant cuscuta reflexa have been found: dna from apical parts of the plant is less methylated (m5c = 4,2 mol %) as compared to the dna from haustoria and posthaustorial regions (m5c = 5,4 mol %). the base compositions of total dna preparations from c. reflexa grown on various hosts are similar. the m5c amount in stem dna of the alfalfa plant infected with c. reflexa is by approximately 25% higher th ...1979454715
changes in polyamine levels on infection of plants by cuscuta reflexa. 19761010580
organization and sequence of photosynthetic genes from the plastid genome of the holoparasitic flowering plant cuscuta reflexa.we have cloned and sequenced an area of about 6 kb of the plastid dna (ptdna) from the holoparasitic plant cuscuta reflexa. this region contains (in the following order) genes for the cytochrome b6 f-complex subunit v (petg), trna(val) (trnv), trna(met) (trnm), the epsilon- and beta-subunit of the chloroplast atp-synthase (atpe and atpb) and the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco; rbcl). in addition we identified other photosynthesis-related genes (atpa, pe ...19921552899
choline kinase in cuscuta reflexa. 19725010855
the purification and nature of an antiviral protein from cuscuta reflexa plants.an antiviral substance, showing highly significant virus inhibiting property, has been isolated from the aqueous extract of cuscuta reflexa plants. the biologically active virus inhibitor was purified by fractionation with organic solvents followed by precipitation with a saturated solution of ammonium sulphate, dialysis, ultracentrifugation and filtration through a sephadex g-200 column. it shows characteristics of proteins with a molecular weight around 14,000-18,000 daltons. the antiviral act ...19817325807
structural and functional analysis of plastid genomes from parasitic plants: loss of an intron within the genus cuscuta.the gene cluster rps12/rps7/psi ndhb of the plastome from the holoparasitic plant cuscuta europaea has been analysed at the nucleotide level. a comparison with the homologous region of the plastome from the closely related parasite cuscuta reflexa reveals a complete loss of the cis-spliced intron of the rps12 gene in addition to a drastic size reduction of the ndhb pseudogene. it is demonstrated by rt-pcr analysis that the entire gene cluster is transcribed in the form of a multicistronic transc ...19957553945
the cdna sequence of cytochrome b5 associated with cytokinin-induced haustoria formation in cuscuta reflexa.a cdna clone isolated by differentially screening a cytokinin-induced haustorial cdna library of cuscuta reflexa was sequenced and identified as the gene coding for cytochrome b5, based on the similarity of the deduced amino-acid sequence with that of the cauliflower (60% identity) and tobacco (78% identity) proteins. the 5'-utr is unusually long (720 bp) and contains 14 potential start codons (atg) and 10 short orfs.19947959021
functional loss of all ndh genes in an otherwise relatively unaltered plastid genome of the holoparasitic flowering plant cuscuta reflexa.we have cloned and sequenced an area of about 9.0 kb of the plastid dna (ptdna) from the holoparasitic flowering plant cuscuta reflexa to investigate the evolutionary response of plastid genes to a reduced selective pressure. the region contains genes for the 16s rrna, a subunit of a plastid nad(p)h dehydrogenase (ndhb), three transfer rnas (trna, trni, trnv) as well as the gene coding for the ribosomal protein s7 (rps7). while the other genes are strongly conserved in c. reflexa, the ndhb gene ...19948111019
cloning and sequence of a cdna encoding a novel hybrid proline-rich protein associated with cytokinin-induced haustoria formation in cuscuta reflexa.a complete cdna encoding a novel hybrid pro-rich protein (hyprp) was identified by differentially screening 3 x 10(4) recombinant plaques of a cuscuta reflexa cytokinin-induced haustorial cdna library constructed in lambda gt10. the nucleotide (nt) sequence consists of: (i) a 424-bp 5'-non coding region having five start codons (atgs) and three upstream open reading frames (uorfs); (ii) an orf of 987 bp with coding potential for a 329-amino-acid (aa) protein of m(r) 35,203 with a hydrophobic n-t ...19948163190
a large deletion in the plastid dna of the holoparasitic flowering plant cuscuta reflexa concerning two ribosomal proteins (rpl2, rpl23), one transfer rna (trni) and an orf 2280 homologue.we have determined the nucleotide sequence of a 5.3-kb region of the plastid dna (ptdna) from the heterotrophic holoparasitic plant cuscuta reflexa. the cloned area contains genes for the d1-protein (32-kda protein; psba), trna(his) (trnh), orf 740 (homologous to orf 2280 from nicotiana tabacum), orf 77 (homologous to orf 70), trna(leu) (trnl) and a hypothetical orf 55 which has no homology to any known gene among higher plants. this 5.3-kb area is colinear with a 12.4-kb region of tobacco ptdna ...19938358824
physico-chemical and functional characterization of a high molecular weight carboxymethylcellulase from cuscuta reflexa.carboxymethylcellulase (cmcase) was extracted and purified from an angiosperm parasite cuscuta reflexa free from beta-glucosidase and other enzyme activities. the molecular mass and stokes' radius of the purified enzyme are 144 kda and 44 a, respectively. the diffusion coefficient and frictional ratio of the enzyme were 5.15 x 10(-7) cm2/sec and 1.27. the sds-page revealed homotetrameric nature of the enzyme with a subunit molecular mass of 35 +/- 1 kda. titration against dtnb and nbs revealed 1 ...19979491645
unusual carotenoid composition and a new type of xanthophyll cycle in plants.the capture of photons by the photosynthetic apparatus is the first step in photosynthesis in all autotrophic higher plants. this light capture is dominated by pigment-containing proteins known as light-harvesting complexes (lhcs). the xanthophyll-carotenoid complement of these lhcs (neoxanthin, violaxanthin, and lutein) is highly conserved, with no deletions and few, uncommon additions. we report that neoxanthin, considered an integral component of lhcs, is stoichiometrically replaced by lutein ...19999927706
molecular, functional and ultrastructural characterisation of plastids from six species of the parasitic flowering plant genus cuscuta.plastids of cuscuta reflexa roxb., c. subinclusa d. et h., c. gronovii willd. and c. campestris yunck. possess thylakoids and contain both chlorophyll a and b in a ratio similar to that of stem tissue of the systematically closely related but 'normal' green ipomoea tricolor. in contrast, plastids of c. odorata r. et p. and c. grandiflora h.b.k. do not contain any chlorophyll or possess thylakoids. light-driven electron transport, as measured by oxygen evolution and indicated by analysis of chlor ...200010805440
macromolecular trafficking between nicotiana tabacum and the holoparasite cuscuta reflexa.transgenic tobacco plants expressing green fluorescent protein (gfp) under the control of the companion cell-specific promoter, atsuc2, were parasitized by the holoparasite cuscuta reflexa (dodder). gfp, moving in the translocation stream of the host, was transferred to the cuscuta phloem via the absorbing hyphae of the parasite. an identical pattern of transfer was observed for the phloem-mobile probe, carboxyfluorescein. following uptake by the parasite, gfp was translocated and unloaded from ...200111181727
characterization of two tomato aquaporins and expression during the incompatible interaction of tomato with the plant parasite cuscuta reflexa.a subtractive suppression hybridization technique was used to identify genes that were induced during early phases of the interaction between cuscuta reflexa, a phanerogamic plant parasite and the incompatible host tomato (lycopersicon esculentum mill.). one of the identified genes encodes a new aquaporin (leaqp2) from tomato. its function was concluded from the swelling kinetics of leaqp2-expressing xenopus laevis oocytes under hypo-osmotic conditions. it was shown that, 6 h after attachment of ...200111556787
alpha-glucosidase inhibitory constituents from cuscuta reflexa.two new compounds, 7'-(3',4'-dihydroxyphenyl)-n-[(4-methoxyphenyl)ethyl]propenamide (4), and 7'-(4'-hydroxy,3'-methoxyphenyl)-n-[(4-butylphenyl)ethyl]propenamide (5) have been isolated from cuscuta reflexa along with five known compounds, 6,7-dimethoxy-2h-1-benzopyran-2-one (1), 3-(3,4-dihydroxyphenyl)-2-propen-1-ethanoate (2), 6,7,8-trimethoxy-2h-1-benzopyran-2-one (3), 3-(4-o-beta-d-glucopyranoside-3,5-dimethoxyphenyl)-2-propen-1-ol (6), 2-(3-hydroxy-4-methoxyphenyl)-3,5-dihydroxy-7-o-beta-d-g ...200211824569
identification of pyrogallol as an antiproliferative compound present in extracts from the medicinal plant emblica officinalis: effects on in vitro cell growth of human tumor cell lines.in this study we compared the in vitro antiproliferative activity of extracts from medicinal plants toward human tumor cell lines, including human erythromyeloid k562, b-lymphoid raji, t-lymphoid jurkat, erythroleukemic hel cell lines. extracts from emblica officinalis were the most active in inhibiting in vitro cell proliferation, after comparison to those from terminalia arjuna, aphanamixis polystachya, oroxylum indicum, cuscuta reflexa, aegle marmelos, saraca asoka, rumex maritimus, lagerstro ...200212063567
blue light-sensitive plasma membrane bound exogenous nadh oxidase in cuscuta reflexa.protoplasts isolated from cuscuta reflexa exhibited a higher rate of exogenous nadh oxidation as compared to nadph in the dark. nad(p)h oxidation was monitored by measuring the rate of oxygen consumption and this oxidase system was sensitive to blue light. both nadh oxidase and its blue light sensitivity were inhibited by -sh group reacting agents. the corresponding changes occurring in h+-extrusion activity and intracellular atp levels were also monitored. stimulation of nadh oxidation under bl ...200012557914
plastid transcription in the holoparasitic plant genus cuscuta: parallel loss of the rrn16 pep-promoter and of the rpoa and rpob genes coding for the plastid-encoded rna polymerase.the holoparasitic plant genus cuscuta comprises a range of species whose plastid genomes have different degrees of reductions in their coding capacity. in this study, four cuscuta species, cuscuta reflexa, c. gronovii, c. subinclusa and c. odorata, that possess substantial physiological differences, were analysed with respect to the sequence and promoter structure of the rrn16 gene coding for the ribosomal 16s rrna. whereas the coding region of this gene is highly conserved among all four cuscut ...200312624769
development of cuscuta species on a partially incompatible host: induction of xylem transfer cells.the growth of dodders, cuscuta reflexa and cuscuta japonica, on the partially incompatible host poinsettia ( euphorbia pulcherrima) is studied. poinsettia responds by bark growths to the formation of the dodder haustoria and prevents dodder from obtaining normal growth. the growth instead becomes extremely branched, coral-like, and dodder lacks the ability to form haustoria. after a period of coral-like growth, long shoots sprout, resembling the normal growth. these long shoots mark an ending ph ...200312664277
plastids of three cuscuta species differing in plastid coding capacity have a common parasite-specific rna composition.the chlorophyll containing holoparasitic species cuscuta reflexa, the achlorophyllous species cuscuta odorata and the intermediate species cuscuta gronovii, which contains only traces of chlorophyll, were compared with respect to their plastid coding capacity and plastid gene expression at the level of rna. while extensive deletions have taken place in the plastid dna of the achlorophyllous species c. odorata, the green species c. reflexa has retained an almost complete plastid genome. although ...200312898255
preliminary pharmacological investigations on cuscuta reflexa roxb. 196514336163
onset of puberty and ovarian steroidogenesis following adminstration of methanolic extract of cuscuta reflexa roxb. stem and corchorus olitorius linn. seed in mice.the effect of methanolic extract (me) of cuscuta reflexa stem roxb. and corchorus olitorius linn. seed on the onset of reproductive maturity and the ovarian steroidogenesis was studied by means of biochemical techniques. me of cuscuta reflexa stem and corchorus olitorius seed treatment causes a remarkable delay in sexual maturation as evidenced by the age at vaginal opening and appearance of first estrus (cornified smear). the same treatment also results in a significant diminution of delta(5)-3 ...200314522432
studies on brain biogenic amines in methanolic extract of cuscuta reflexa roxb. and corchorus olitorius linn. seed treated mice.the methanolic extract of both cuscuta reflexa stem and corchorus olitorius seed showed marked protection against convulsion induced by chemoconvulsive agents in mice. the catecholamines contained were significantly increased in the processed extract treated mice. the amount of gaba, which is most likely to be involved in seizure activity, was increased significantly in mice brain after a six week treatment. results of the present study revealed that both the processed extracts showed a signific ...200314556490
carotenoid specificity of light-harvesting complex ii binding sites. occurrence of 9-cis-violaxanthin in the neoxanthin-binding site in the parasitic angiosperm cuscuta reflexa.the parasitic angiosperm cuscuta reflexa has a highly unusual carotenoid composition in that it does not contain neoxanthin, an otherwise ubiquitous component of the major light-harvesting complex protein (lhciib) in all other higher plant species studied to date. combined hplc and mass spectrometric analysis has enabled us to detect in tissues of c. reflexa two new types of xanthophylls: lutein-5,6-epoxide and 9-cis-violaxanthin. we have isolated the lhciib complex from thylakoids and analyzed ...200414610095
chemical and toxicological evaluation of methanol extract of cuscuta reflexa roxb. stem and corchorus olitorius linn. seed on hematological parameters and hepatorenal functions in mice.methanol extract of cuscuta reflexa roxb. stem (mecr) contain flavonoids (0.2%) and corchorus olitorius linn. seed (meco) was found to contain steroids and cardenolide glycosides. effects of multiple weekly dose of mecr (25, 50, 75 mg/kg, i.p.) and meco (15, 20, 25 mg/kg, i.p.) on liver and kidney functions and hematological parameters in mice were studied. no significant alteration of rbc count and hemoglobin content was observed in all dose level of treatment in mecr and meco treated mice wher ...200314714862
evaluation of psychopharmacological effects of petroleum ether extract of cuscuta reflexa roxb. stem in mice.the petroleum ether extract of cuscuta reflexa roxb. stem (pecr) was evaluated for its psychopharmacological activities in several experimental models using swiss albino mice. the pecr was found to cause significant reduction in spontaneous activity and exploratory behavioral profiles. it also showed reduction in muscle relaxant activity by rotarod, 30 degrees inclined screen tests and showed significant analgesic properties as well as potentiated remarkably the pentobarbitone sodium, diazepam a ...200315080597
the cell wall-modifying xyloglucan endotransglycosylase/hydrolase lexth1 is expressed during the defence reaction of tomato against the plant parasite cuscuta reflexa.a suppressive subtractive hybridization technique was used to identify genes, which were induced during the early phases of the interaction between dodder (cuscuta reflexa), a phanerogamic parasite, and its incompatible host plant tomato. one of the identified genes encodes a tomato xyloglucan endotransglycosylase/hydrolase (xth)--an enzyme involved in cell wall elongation and restructuring. the corresponding lexth1 mrna accumulated 6 h after attachment of the parasite. in contrast, wounding did ...200415248122
synthesis of phytochelatins and modulation of antioxidants in response to cadmium stress in cuscuta reflexa--an angiospermic parasite.effect of cadmium on growth, antioxidative enzymes namely catalase, peroxidase, glutathione reductase, level of glutathione and phytochelatin synthesis was investigated in callus and seedlings of cuscuta reflexa. a time, concentration and tissue dependent response of cd was observed. cd inhibited the growth of callus and seedlings by 50% at 300 and 500 micromol/l concentrations, respectively. shorter exposure of low concentration of cd led to augmentation of antioxidant activity, both in callus ...200415266713
anti-steroidogenic activity of methanolic extract of cuscuta reflexa roxb. stem and corchorus olitorius linn. seed in mouse ovary.methanolic extract (me) of both c. reflexa stem and c. olitorius seed arrested the normal oestrus cycle of adult female mouse and significantly decreased the weight of ovaries and uterus. the cholesterol and ascorbic acid contents in ovaries were significantly increased in the treated mice. two key enzymes, delta5-3beta-hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase, were decreased significantly in me of both c. reflexa stem and c. olitorius seed after 17 days of treatment. h ...200315266914
a new flavanone, reflexin, from cuscuta reflexa and its selective sensing of nitric oxide.a new compound, reflexin, 5-hydroxy-7-methoxy-6-(2,3-epoxy-3-methylbutyl)-flavanone, is isolated from the stems of cuscuta reflexa along with three other known compounds. this new compound has good potential for application especially in the photoactivity of reflexin. it was found to be sensitive to glutathione, forming a fluorescent product that is utilized for sensing nitric oxide (no). the lowest detection limit of no analysis was found to be 0.05 micro/m.200516186624
trehalose toxicity in cuscuta reflexa: correlation with low trehalase activity.a toxic effect of alpha,alpha-trehalose in an angiospermic plant, cuscuta reflexa (dodder), is described. this disaccharide and its analogs, 2-aminotrehalose and 4-aminotrehalose, induced a rapid blackening of the terminal region of the vine which is involved in elongation growth. from the results of in vitro growth of several angiospermic plants and determination of trehalase activity in them, it is concluded that the toxic effect of trehalose in cuscuta is because of the very low trehalase act ...198116662110
trehalose toxicity in cuscuta reflexa: sucrose content decreases in shoot tips upon trehalose feeding.trehalose, an alpha,alpha-diglucoside, induced a rapid blackening and death of shoot tips of cuscuta reflexa (dodder) cultured in vitro. the onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of cuscuta was supplied with trehalose. the rate of trehalose uptake or its accumulation in the tissue was not affected by sugar cofeeding. the levels of total and reducing sugars declined appreciably in the trehalose-fed shoot tip explants compared to control ...198216662380
trehalose toxicity in cuscuta reflexa: cell wall synthesis is inhibited upon trehalose feeding.alpha,alpha-trehalose induced a rapid blackening of the terminal 2.5-centimeter region of excised cuscuta reflexa roxb. vine. the incorporation of radioactivity from [(14)c]glucose into alkali-insoluble fraction of shoot tip was markedly inhibited by 12 hours of trehalose feeding to an excised vine. this inhibition was confined to the apical segment of the vine in which cell elongation occurred. the rate of blackening of shoot tip explants was hastened by the addition of gibberellic acid a(3), w ...198216662557
antibacterial activity of cuscuta reflexa stem and corchorus olitorius seed.the methanol extracts of cuscuta reflexa stem and corchorus olitorius seed showed a broad spectrum of antibacterial activity.200616890386
an attack of the plant parasite cuscuta reflexa induces the expression of attagp, an attachment protein of the host tomato.dodder or cuscutaceae are holoparasitic plants subsisting on other dicotyledonous plants. the infection process is initiated by adherence of cuscuta prehaustoria to the host surface, followed by penetration attempts by hyphae. in the case of a successful infection, these organs connect the parasite's vascular tissue to that of the host. here we show that contact of cuscuta reflexa prehaustoria to tomato induces the expression of a new arabinogalactan protein (agp), attagp, in the tomato precisel ...200617076801
development and evaluation of polyherbal formulation for hair growth-promoting activity.cuscuta reflexa (roxb.), citrullus colocynthis (schrad.), and eclipta alba (hassk.) are traditionally acclaimed herbs for their hair growth-promoting potential. aim in the present study, it was envisaged to prepare herbal formulations containing petroleum ether extracts of the three herbs in varying ratio and evaluating the formulations for the hair growth-promoting activity.200717524127
arabidopsis thaliana is a susceptible host plant for the holoparasite cuscuta spec.arabidopsis thaliana and cuscuta spec. represent a compatible host-parasite combination. cuscuta produces a haustorium that penetrates the host tissue. in early stages of development the searching hyphae on the tip of the haustorial cone are connected to the host tissue by interspecific plasmodesmata. ten days after infection, translocation of the fluorescent dyes, texas red (tr) and 5,6-carboxyfluorescein (cf), demonstrates the existence of a continuous connection between xylem and phloem of th ...200717598126
swarnalin and cis-swarnalin, two new tetrahydrofuran derivatives with free radical scavenging activity, from the aerial parts of cuscuta reflexa.the reversed-phase hplc analysis of a methanol extract of the aerial parts of cuscuta reflexa afforded a non-separable mixture (55 : 45) of two novel tetrahydrofuran derivatives, named swarnalin (1) and cis-swarnalin (2), and a known coumarin, 5,6,7-trimethoxycoumarin (3). the structures of the compounds were elucidated unequivocally by uv, hrfabms and a series of 1d and 2d nmr analyses. the mixture of 1 and 2 showed significant free radical scavenging activity in the dpph assay and the rc50 val ...200717613825
complete dna sequences of the plastid genomes of two parasitic flowering plant species, cuscuta reflexa and cuscuta gronovii.the holoparasitic plant genus cuscuta comprises species with photosynthetic capacity and functional chloroplasts as well as achlorophyllous and intermediate forms with restricted photosynthetic activity and degenerated chloroplasts. previous data indicated significant differences with respect to the plastid genome coding capacity in different cuscuta species that could correlate with their photosynthetic activity. in order to shed light on the molecular changes accompanying the parasitic lifesty ...200717714582
evidence for abscisic acid biosynthesis in cuscuta reflexa, a parasitic plant lacking neoxanthin.abscisic acid (aba) is a plant hormone found in all higher plants; it plays an important role in seed dormancy, embryo development, and adaptation to environmental stresses, most notably drought. the regulatory step in aba synthesis is the cleavage reaction of a 9-cis-epoxy-carotenoid catalyzed by the 9-cis-epoxy-carotenoid dioxygenases (nceds). the parasitic angiosperm cuscuta reflexa lacks neoxanthin, one of the common precursors of aba in all higher plants. thus, is c. reflexa capable of synt ...200818441226
effect of cuscuta reflexa roxb on androgen-induced alopecia.alopecia is a psychologically distressing condition. androgenetic alopecia, which affects millions of men and women, is an androgen-driven disorder. here, cuscuta reflexa roxb is evaluated for hair growth activity in androgen-induced alopecia.200818789055
cucumber, melon, pumpkin, and squash: are rules of editing in flowering plants chloroplast genes so well known indeed?the similarities and differences in the chloroplast genes editing patterns of four species from one family (and two genera), which is the first-ever attempt at comparison of such data in closely related species, is discussed. the effective use of the chloroplast genes editing patterns in evolutionary studies, especially in evaluating the kinship between closely related species, is thereby proved. the results indicate that differences in editing patterns between different genera (cucumis and cucu ...200919162145
cuscuta reflexa invasion induces ca release in its host.cuscuta reflexa induces a variety of reaction in its hosts. some of these are visual reactions, and it is clear that these morphological changes are preceded by events at the molecular level, where signal transduction is one of the early processes. calcium (ca(2+)) release is the major second messenger during signal transduction, and we therefore studied ca(2+) spiking in tomato during infection with c. reflexa. bioluminescence in aequorin-expressing tomato was monitored for 48 h after the onset ...201020522193
calcium signaling during the plant-plant interaction of parasitic cuscuta reflexa with its hosts.the plant parasite cuscuta reflexa induces various responses in compatible and incompatible host plants. the visual reactions of both types of host plants including obvious morphological changes require the recognition of cuscuta ssp. a consequently initiated signaling cascade is triggered which leads to a tolerance of the infection or, in the case of some incompatible host plants, to resistance. calcium (ca(2+)) release is the major second messenger during signal transduction. therefore, we hav ...201020818172
significance of cuscutain, a cysteine protease from cuscuta reflexa, in host-parasite interactions.plant infestation with parasitic weeds like cuscuta reflexa induces morphological as well as biochemical changes in the host and the parasite. these modifications could be caused by a change in protein or gene activity. using a comparative macroarray approach cuscuta genes specifically upregulated at the host attachment site were identified.201020964874
in vitro anti-inflammatory and anti-cancer activities of cuscuta reflexa roxb.to determine anti-inflammatory and anti-cancer activities of cuscuta reflexa in cell lines (in vitro).201121295129
effect of cuscuta reflexa stem and calotropis procera leaf extracts on glucose tolerance in glucose-induced hyperglycemic rats and mice.cuscuta reflexa (whole plant) and calotropis procera (leaves) are used in folk medicine of bangladesh to control blood sugar in patients suffering from diabetes mellitus. the hypoglycemic effects of methanol and chloroform extracts of whole plants of cuscuta reflexa, and methanol extract of leaves of calotropis procera were investigated in oral glucose tolerance tests in long evans rats and swiss albino mice, respectively. both methanol and chloroform extracts of cuscuta reflexa whole plant demo ...200921304621
Documentation and determination of consensus about phytotherapeutic veterinary practices among the Tharu tribal community of Uttar Pradesh, India.PURPOSE: The aim of the present investigation was to document the phytotherapeutic knowledge and veterinary healthcare management practices among the Tharu tribal community of Uttar Pradesh, India and to determine the consensus of such practices, in order to evaluate the potential for new veterinary drugs of herbal origin. METHODS: This study was conducted in 2000-2004 using semistructured, open-ended questionnaires, informal interviews and group discussions with farmers engaged in animal husban ...201121927989
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