| pig-bel--a zoonosis? | it is postulated that the combination of a very low protein diet, with low pancreatic tryptic activity, plus the continual consumption of sweet potato trypsin inhibitors leads to inadequate proteolysis when a meal containing meat contaminated with c. perfringens type c and its beta toxin is consumed. the necrotizing pathogenic process concluding as segmental gangrene is set in motion by toxin produced under altered dietary conditions first by motor paralysis via beta toxin, followed by villous a ... | 1978 | 731766 |
| synthesis and turnover of phenylalanine ammonia-lyase in root tissue of sweet potatoe injured by cutting. | 1. antibody toward phenylalanine ammonia-lyase of root tissue of sweet potato injured by cutting was obtained by immunizing of a rabbit with purified enzyme. 2. phenylalanine ammonia-lyase activity developed markedly in disks of sweet potato roots in response to cut injury, reached a maximum after 12 h, and then decreased thereafter. phenylalanine ammonia-lyase content, which was determined by the quantitative immunoprecipitation method, changed in parallel with enzyme activity. the rate of inco ... | 1977 | 837940 |
| bacterial stalk rot of maize, its symptoms and host-range. | stalk rot of maize, caused by erwinia carotovora f. sp. zeae sabet (re-designated as pectobacterium chrysanthemi pathovar. zeae by kelman 1974) showed first premature withering and drying up of the uppermost leaves which was soon followed by the lower leaves. the rot either extended from the base upwards (basal rot) or from the top downwards (top rot). in the case of basal rot, the leaves become yellow and the infected tissue becomes brown, soft, and water soaked. internally, the stalk turns int ... | 1977 | 857508 |
| immunochemical studies on fluctuation of phenylalanine ammonia-lyase activity in sweet potato in response to cut injury. | antibody toward phenylalanine ammonia-lyase (pal) [ec 4.3.1.5] was obtained by immunization of a rabbit with highly purified pal. the antibody reacted specifically with ral, as demonstrated by the ouchterlony double diffusion test, immunoelectrophoresis, and sds polyacrylamide gel electrophoresis of the immunoprecipitate. experiments using anti-pal showed that pal was not present in fresh sweet potato tissue, but appeared in response to cut injury, reaching a maximum, and then decreasing, in par ... | 1976 | 939758 |
| toxigenic fungi in food. | forty-five fungal isolates from moldy supermarket foods were tested for toxicity to brine shrimp, and twenty-two of these isolates were subsequently tested for toxicity to chicken embryos. highly toxigenic fungi were cladosporium sphaerospermum from a bakery product, fusarium oxysporum from carrots, f. solani from cabbage, aspergillus niger and penicillium corylophilum from bread, p. cyclopium and p. herguei from corn meal, p. lanosum from onions,p. steckii from chocolate syrup, penicillium sp. ... | 1975 | 1147614 |
| construction of hybridomas secreting monoclonal antibody against sweet potato feathery mottle virus and use of antibody for detection of spfmv. | two hybridomas stably secreting monoclonal antibodies (mcab) against sweet potato feathery mottle virus (spfmv) were constructed by fusing mouse myeloma (sp2/0-ag14) with mouse splenocytes immunized with spfmv-lf. the mcab secreted by the two hybridomas specifically reacted with both spfmv-lf (china strain) and spfmv-rc (american strain) in dot-blot-elisa. a large amount of mcab was produced with balb/c mice. the titer of hybridoma cultured supernatant was 1:256, and that of the ascitic fluids w ... | 1992 | 1343832 |
| a nuclear gene encoding beta-amylase of sweet potato. | a nuclear amyb gene from sweet potato encoding beta-amylase (beta amy) that is abundant in tuberous roots and inducible in other organs by an exogenous supply of sucrose or polygalacturonic acid, was isolated and characterized. genomic southern blot hybridization, restriction maps of independently isolated phage lambda genomic clones, and the nucleotide sequence of amyb compared with that of the cdna, all suggested that beta amy of sweet potato is encoded by a gene that is present in a single co ... | 1992 | 1383095 |
| a simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver h(+)-atp synthase and determination of the complete amino acid sequence of epsilon-subunit. | the rat liver mitochondrial epsilon-subunit, coupling factor 6, subunit d, and subunit e of h(+)-atp synthase, which are all extra subunits with no counterparts in escherichia coli, were purified by reverse-phase high performance liquid chromatography. the complete amino acid sequence of the rat epsilon-subunit was determined by automated edman degradation of the whole protein and derived peptides. the protein contains 50 amino acids and has a molecular mass of 5635 kda. it is a basic hydrophili ... | 1992 | 1429613 |
| maternity and medicinal plants in vanuatu. i. the cycle of reproduction. | ethnobotanical data collection to select pharmacologically active species was carried out within a clearly defined therapeutic context: those plants used during the course of a woman's reproductive life. extensive bibliographical and field data collection and cross-examination of the information thus gathered have provided us with a clearer picture of the effectiveness of these plant species. various concepts, behaviours and practices relating to menstruation, pregnancy, birth and birth control ... | 1992 | 1453707 |
| the nuclear factor sp8bf binds to the 5'-upstream regions of three different genes coding for major proteins of sweet potato tuberous roots. | sporamin and beta-amylase are two major proteins of tuberous roots of sweet potato, and expression of genes coding for sporamin and beta-amylase is induced concomitantly in leaves with the petioles attached by exogenous supply of sucrose or polygalacturonic acid. we have used a dnase i footprinting assay to characterize nuclear factors that bind to the 5'-upstream regions of gspo-a1, gspo-b1 and g beta-amy genes that encode a-type sporamin, b-type sporamin and the subunit of beta-amylase, respec ... | 1992 | 1531033 |
| dietary intake of vitamin a in african pre-school children in umlazi, south africa. | vitamin a deficiency has been shown to have an impact on morbidity and mortality and therefore prevalence rates are important for public health policy. we report the use of a 7-day recall, dietary questionnaire, which was validated and administered to 203 randomly selected urban african pre-school children in umlazi, south africa. common vitamin a containing foods eaten were leafy green vegetables, pumpkin, sweet potato, mangoes and chicken liver. although the mean daily intake of vitamin a was ... | 1992 | 1587704 |
| dna relatedness among strains of the sweet potato pathogen streptomyces ipomoea (person and martin 1940) waksman and henrici 1948. | dna relatedness among 28 putative strains of streptomyces ipomoea from geographically diverse locations and the type strain, nrrl b-12321, was determined spectrophotometrically. the data confirm that these 28 strains are not closely related genetically to the plant-pathogenic species streptomyces scabies (39% dna relatedness) or streptomyces acidiscabies (17% dna relatedness) or any other major blue-spored streptomyces species (less than 30% dna relatedness). of the 29 strains examined, 4 could ... | 1992 | 1610178 |
| dna-dna hybridization in the systematics of streptomyces. | the dna relatedness among strains in several different phenotypically defined streptomyces species clusters was evaluated. it was found that the data from dna-relatedness studies do not necessarily agree with the clustering generated using numerical taxonomic techniques. a study of the morphologically heterogeneous 's. cyaneus' cluster showed that morphological criteria traditionally used to classify and identify streptomyces species still have value, since strains in dna-relatedness cluster gro ... | 1992 | 1612444 |
| acute interstitial pneumonia in cattle associated with consumption of mould-damaged sweet potatoes (ipomoea batatas). | | 1992 | 1632730 |
| the effect of diet on microfaunal population and function in the caecum of a subterranean naked mole-rat, heterocephalus glaber. | the effect of dietary fibre and starch content on digestibility, microfaunal population and caecal function was investigated in a subterranean mole-rat, heterocephalus glaber (rodentia). mole-rats were fed on a diet of either sweet potato (neutral-detergent fibre (ndf) 65 g/kg dry matter (dm), starch 638 g/kg dm) or carrot (ndf 157 g/kg dm, starch 258.7 g/kg dm) for 4 weeks. daily intake and faecal output were monitored. thereafter caecal microfaunal population, density and function were assesse ... | 1991 | 1645994 |
| studies on aldose reductase inhibitors from natural products. iv. constituents and aldose reductase inhibitory effect of chrysanthemum morifolium, bixa orellana and ipomoea batatas. | the hot water extracts of chrysanthemum morifolium, bixa orellana and ipomoea batatas, were found to have potent inhibitory activity towards lens aldose reductase (ar). ellagic acid (4) was isolated from c. morifolium and i. batatas, isoscutellarein (7) from b. orellana and 3,5-dicaffeoylquinic acid (10) from i. batatas, respectively, as potent inhibitors. | 1991 | 1814628 |
| kinetics and mathematical model of ethanol formation by immobilized yeast cells. | kinetics of the ethanol fermentation with the immobilized k yeast cells was studied both experimentally and theoretically. a kinetic model consisting of 6 equations was proposed. in this model the combined inhibition, i.e., glucose in uncompetitive inhibition and ethanol in noncompetitive inhibition with irreversible performance, was taken into consideration. calculated values agreed approximately with the experimental results, and this model was found to be of practical use. the maximum average ... | 1991 | 1823593 |
| molecular cloning and expression in escherichia coli of cdna encoding the subunit of sweet potato beta-amylase. | tuberous roots of the sweet potato are unusually rich in beta-amylase, and the beta-amylase polypeptides account for about 5% of the total soluble protein of the organ. unlike beta-amylases from other origins, the sweet potato beta-amylase is a tetramer of identical subunits, and it also bears starch phosphorylase-inhibitor activity. a cdna for the subunit of sweet potato beta-amylase was obtained by immunological screening of an expression cdna library constructed by the vector-primer and linke ... | 1991 | 1837016 |
| ubiquinol-cytochrome c oxidoreductase of higher plants. isolation and characterization of the bc1 complex from potato tuber mitochondria. | a procedure is described for isolation of active ubiquinol-cytochrome c oxidoreductase (bc1 complex) from potato tuber mitochondria using dodecyl maltoside extraction and ion exchange chromatography. the same procedure works well with mitochondria from red beet and sweet potato. the potato complex has at least 10 subunits resolvable by gel electrophoresis in the presence of dodecyl sulfate. the fifth subunit carries covalently bound heme. the two largest ("core") subunits either show heterogenei ... | 1991 | 1851164 |
| propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. | sporamin is a protein without glycans that accumulates in large quantities in the vacuoles of the tuberous root of the sweet potato. it is synthesized as a prepro precursor with an n-terminal extension composed of a 21-amino-acid signal peptide and a 16-amino-acid propeptide. a total of 48 base pairs, corresponding to the nucleotide sequence that encodes the propeptide, was deleted from a cdna clone for sporamin. this delta pro mutant sequence, as well as the sequence of the wild-type sporamin c ... | 1991 | 1992474 |
| high-level expression of a sweet potato sporamin gene promoter: beta-glucuronidase (gus) fusion gene in the stems of transgenic tobacco plants is conferred by multiple cell type-specific regulatory elements. | genes coding for sporamin, the most abundant protein of the tuberous root of the sweet potato, are expressed at a high levels in the stems of plantlets cultured axenically on sucrose-containing medium. their expression is also induced in leaf-petiole explants by high concentrations of sucrose. a fusion gene comprising of the 1 kb 5' upstream region of the gspo-a1 gene coding for the a-type sporamin and the coding sequence of bacterial beta-glucuronidase (gus) was introduced into the tobacco geno ... | 1991 | 2017135 |
| high-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium. | sporamin, the tuberous root storage protein of the sweet potato, accounts for about 60 to 80% of the total soluble protein of this organ. the amount of sporamin present in other organs is very low, or even not detectable, in the normal field-grown plants. however, the stem of sweet potato plantlets grown axenically on agar medium containing sucrose was found to accumulate large amounts of sporamin. two-dimensional gel electrophoretic profiles of sporamin precursors synthesized in vitro by poly(a ... | 1990 | 2102838 |
| the plant mitochondrial f1-atpase. the identity of the delta' (20 kda) subunit. | the n-terminal amino acid sequence of the 20 kda (delta') subunit of the turnip (brassica napus l.) mitochondrial f1-atpase has been determined. comparison of the sequence obtained with those of the epsilon subunits of chloroplast cf1, e. coli f1 and the delta subunit of bovine f1 shows that the turnip delta' subunit is another member of this family of homologous proteins. the delta' subunit of sweet potato f1-atpase [(1989) j. biol. chem. 264, 3183-3186] is very similar to the turnip sequence a ... | 1990 | 2147662 |
| alteration of the properties of aspergillus sp. k-27 glucoamylase on limited proteolysis with subtilisin. | an active derivative (mol. wt. 48,000) of aspergillus sp. k-27 glucoamylase (mol. wt. 76,000) was obtained by limited proteolysis with subtilisin. the amino acid sequences of native and modified enzymes at the n-termini were ala-gly-gly-thr-leu-asp and ala-val-leu, respectively. the proteolysis greatly decreased the affinity of the enzyme for amylopectin and glycogen, but not for oligosaccharides. it also reduced the ability of the enzyme to degrade raw starch, abolished the ability of the enzym ... | 1990 | 2224899 |
| vacuolar targeting and posttranslational processing of the precursor to the sweet potato tuberous root storage protein in heterologous plant cells. | sporamin, the tuberous root storage protein of the sweet potato, which is localized in vacuoles, is synthesized as a prepro-precursor with an n-terminal sequence of amino acids that includes a signal peptide and an additional pro-segment of 16 amino acids. a full-length cdna for sporamin was placed downstream of the 35 s promoter of cauliflower mosaic virus and introduced into tobacco and sunflower genomes by ti plasmid-mediated transformation. a polypeptide of nearly the same size as mature spo ... | 1990 | 2246259 |
| characterization of a cdna encoding cottonseed catalase. | a 1.7 kb cdna clone was isolated from our lambda gt11 library constructed from poly(a) rna of 24-h-old cotyledons. the cdna encodes a full-length catalase peptide (492 amino acid residues). the calculated molecular mass is 56,800, similar to that determined for purified enzyme (57,000 sds-page). among higher plant catalases, this cotton catalase shows the highest amino acid sequence identity (85%) to the subunit of homotetrameric maize cat 1, a developmental counterpart to the homotetrameric cat ... | 1990 | 2364113 |
| characterization of catalase transcripts and their differential expression in maize. | in maize, the three unlinked catalase (ec 1.11.1.6) structural genes (cat1, cat2 and cat3) are differentially expressed temporally, spatially and in response to environmental signals in the developing seedling. in order to understand more fully the molecular mechanisms involved in catalase gene expression, full-length cdna clones representing the maize cat1, cat2 and cat3 transcripts were isolated and characterized. dna sequence analysis confirmed that each cdna encodes a unique catalase protein ... | 1988 | 2461221 |
| possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. | there are at least ten viruses identified in the literature that resemble definitive potyviruses in having flexuous filamentous particles and inducing the formation of "pinwheel" cytoplasmic inclusions in infected cells but that are transmitted by eriophyid mites, whiteflies or soil fungi and not by aphids, the vectors of the definitive potyviruses. the taxonomic status of these viruses is uncertain at present. using a broadly cross-reactive antiserum raised against the dissociated coat protein ... | 1989 | 2473719 |
| correspondence of minor subunits of plant mitochondrial f1atpase to f1f0atpase subunits of other organisms. | in addition to two major alpha- and beta-subunits, the soluble oligomycin-insensitive f1atpase purified from sweet potato root mitochondria contains four different minor subunits of gamma (mr = 35,500), delta (mr = 27,000), delta' (mr = 23,000), and epsilon (mr = 12,000) (iwasaki, y., and asashi, t. (1983) arch. biochem. biophys. 227, 164-173). among these minor subunits, the delta-subunit specifically cross-reacted with an antibody against the delta-subunit of maize mitochondrial f1 which conta ... | 1989 | 2536736 |
| expression and processing of cyanobacterial mn-stabilizing protein in escherichia coli. | the woxa gene of cyanobacterium anacystis nidulans r2, which encodes the precursor of the mn-stabilizing protein involved in photosynthetic water oxidation, was found to be expressed in escherichia coli. the 30-kda expression product was indistinguishable from the authentic mature protein on sds/page. upon fractionation of e. coli cells, the expression product was co-precipitated with the membrane fraction, which is consistent with the water-insoluble nature of the authentic mature protein. anal ... | 1989 | 2689172 |
| partial cleavage of sweet potato feathery mottle virus coat protein subunit by an enzyme in extracts of infected symptomless leaves. | the coat protein of particles of sweet potato feathery mottle potyvirus (spfmv) extracted from ipomoea spp. migrated in sds-page mainly as bands of m(r) 38,000 (38k), 36k, 32k, 30k. trypsin treatment of the particles resulted in the appearance of only one 30k polypeptide. the inclusion of protease inhibitors in the extraction procedure did not alter the heterogeneity of spfmv coat protein. a partially purified fraction of extracts from recovering, symptomless, but not from healthy leaves of i. n ... | 1989 | 2769226 |
| parotid gland enlargement and female reproductive performance in a papua new guinea highland population. | swelling of the parotid salivary glands is commonly observed among males and females of highland papua new guinea. evidence suggests that the condition is related to the highlanders' high starch consumption in the form of sweet potato. in this paper we regard parotid enlargement as an indicator of high energy intake and test its association with two measures of reproductive performance, the number of live births and living offspring, in a sample of 274 women from lufa in the eastern highlands di ... | 1989 | 2802523 |
| molecular cloning and nucleotide sequence of full-length cdna for sweet potato catalase mrna. | a nearly full-length cdna clone for catalase (pcas01) was obtained through immunological screening of cdna expression library constructed from size-fractionated poly(a)-rich rna of wounded sweet potato tuberous roots by escherichia coli expression vector-primed cdna synthesis. two additional catalase cdna clones (pcas10 and pcas13), which contained cdna inserts slightly longer than that of pcas01 at their 5'-termini, were identified by colony hybridization of another cdna library. those three ca ... | 1987 | 2885193 |
| processing of a plant vacuolar protein precursor in vitro. | a precursor for sporamin a, the storage protein of the tuberous roots of sweet potato deposited in the vacuole, is synthesized on membrane-bound polysomes and has an extra peptide of 37 amino acids at the n-terminus of the mature form, which can be divided into an n-terminal putative signal peptide sequence (residues -37 to -17) and a segment enriched with charged amino acids (residues -16 to -1) [hattori, t., et al. (1985) plant mol. biol. 5, 313-320]. we examined the in vitro processing of the ... | 1987 | 3301345 |
| quality improvement of sweet-potato (ipomoea batatas l. lam.) roots as feed by ensilage. | 1. sweet-potato (ipomoea batatas l. lam.) strips (sps) mixed with maize powder (cp) in proportions 10:0, 9:1, 8:2, and 7:3 were ensiled for 1, 2 or 3 months. 2. trypsin inhibitor activity (tia) decreased during ensilage in samples of all treatments while the sps-cp mixture (7:3, w/w) ensiled for 3 months contained the lowest tia. 3. sps-cp (8:2, w/w) dried or ensiled for 2 months, or ensiled for 2 months and dried, were each mixed with twice the amount of control diet (1:2, w/w) to make three di ... | 1988 | 3408700 |
| protease production with sweet potato residue by solid state fermentation. | the sweet potato residue being at the initial moisture content 50-58%, initial ph 3.5-4.3, supplemented with rice bran, and minerals, and incubated at 20-26 degrees c for 5 days was the optimal conditions for protease production with aspergillus niger ntu-am-1 by solid state fermentation. protease could be recovered by shaking at room temperature for one hour and extracted with five times volume of 0.1% nacl solution. the yield of protease was 814 units per gram dry weight of substrate. partiall ... | 1986 | 3545704 |
| effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse. | to investigate the effects of consuming a variety of common vegetables on the activities of xenobiotic-metabolizing enzymes in the mouse liver, male c57bl/6 mice were fed purified diets supplemented isocalorically with 20% freeze-dried powdered kidney bean, soya bean, alfalfa, cauliflower, mustard greens, carrot, kale, brussels sprouts, beet, egg plant or onion or with 40% sweet potato. hepatic ethoxycoumarin o-deethylase (ecd) activity was increased significantly (p less than or equal to 0.05) ... | 1985 | 3877667 |
| the nephrotoxicity for mice of deisopropylngaione, a minor furanoid component of toxic myoporaceous essential oils. | deisopropylngaione (din) is one of a family of hepatotoxic furanosesquiterpenoid essential oils which is found in small amounts (5%) in the leaves of some specimens of the australian plant myoporum deserti. din differs from other furanoid myoporaceous essential oils in that it also causes lesions in the lungs and kidneys. at the near ld50 dose rate of 150 mg kg-1 given by intraperitoneal injection, din is able to cause lethal renal proximal tubular necrosis without causing significant injury to ... | 1985 | 3989217 |
| effect of gossypol on some oxidative respiratory enzymes. | gossypol was examined in relation to its effect on certain enzymes and enzyme complexes associated with the tricarboxylic acid cycle and the electron transport system. succinic dehydrogenase and cytochrome oxidase activity from sweet potato was completely inhibited by gossypol at 7.5 x 10(-3)m and 2.0 x 10(-3)m, respectively. succinoxidase activity of the same preparations was fully inhibited at a lower concentration, 2.5 x 10(-4)m. this concentration did not affect either succinic dehydrogenase ... | 1966 | 4287149 |
| effect of triton x-100 on sweet potato -amylase. | | 1972 | 4622930 |
| the action of sweet potato -amylase on glycogen and amylopectin: formation of a novel limit dextrin. | | 1971 | 5165519 |
| toxicity of mould-damaged sweet potatoes (ipomoea batatas). | | 1970 | 5428477 |
| isolation of furanoterpene-containing particles from ceratocystis fimbriata-infected sweet potato root tissue. | furanoterpene-containing particles were isolated by centrifugation on a discontinuous ficoll density gradient from a homogenate of the non-infected tissue adjacent to the infected region of ceratocystis fimbriata-infected sweet potato root tissue. the particles were recovered at a relatively high ratio in the 2% ficoll fraction, in which there was no contamination by mitochondria and only little by endoplasmic reticulum judging from the distribution of the activities of their marker enzymes and ... | 1984 | 6099359 |
| inhibition of electron transport and oxidative phosphorylation in plant mitochondria by gladiolic acid and structurally-related aromatic ortho dialdehydes. | gladiolic acid (ga, 4-methoxy-5-methyl-0-phthalaldehyde-3-carboxylic acid), an antifungal aromatic ortho dialdehyde produced by penicillium gladioli was found to be a potent inhibitor of electron transport and oxidative phosphorylation reactions in sweet potato and mung bean mitochondria. similar results were also found with the naturally occurring ortho dialdehydes, cyclopaldic acid, quadrilineatin, and flavipin as well as the synthetic dialdehydes, 3-formyl opianic acid and 0-phthalaldehyde. b ... | 1980 | 6248181 |
| experimental pigbel: the production and pathology of necrotizing enteritis due to clostridium welchii type c in the guinea-pig. | an animal model for pigbel in man was developed using guinea-pigs. intragastric dosing with growing cultures of clostridium welchii type c only produced necrotic lesions if protease inhibitors were given as well. beta toxin, which is made by the type c organism, causes the intestinal damage and is very easily destroyed by proteases. protease inhibitors in soybean and aprotinin were effectively in inducing disease in animals on a normal diet, while inhibitors in sweet potato, which inhibit only t ... | 1980 | 6252934 |
| cholesterol binding capacity of fiber from tropical fruits and vegetables. | the cholesterol binding capacity of 28 fiber samples from a variety of the more common tropical fruits and vegetables was determined. the binding capacity of cholestyramine, cellulose, lignin, guar gum and citrus pectin were also determined. capacities were evaluated by an in vitro method that simulates the effect of the human digestive system on fiber using a series of enzymatic treatments before the binding was determined. binding values varied from 3% for a soluble fraction of cassava to 84% ... | 1984 | 6323908 |
| protein nutritional value of sweet potato flour. | | 1983 | 6415140 |
| production of oxalic acid by some fungi infected tubers. | oxalic acid (as oxalate) was detected in four tubers commonly used for food in nigeria-dioscorea rotundata (white yam), solanum tuberosum (irish potato), ipomoea batatas (sweet potato), and manihot esculenta (cassava). whereas healthy i. batata had the highest oxalic acid content, healthy m. esculenta contained the lowest. when all tubers were artifically inoculated with four fungi-penicillium oxalicum curie and thom, aspergillus niger van tiegh, a. flavus and a. tamarii kita, there was an incre ... | 1983 | 6670294 |
| biological quality and composition of sweet potato protein fragments. | | 1981 | 7276386 |
| cloning of the cdna for the human atp synthase oscp subunit (atp5o) by exon trapping and mapping to chromosome 21q22.1-q22.2. | exon trapping was used to clone portions of potential genes from human chromosome 21. one trapped sequence showed striking homology with the bovine and rat atp synthase oscp (oligomycin sensitivity conferring protein) subunit. we subsequently cloned the full-length human atp synthase oscp cdna (gdb/hgmw approved name atp50) from infant brain and muscle libraries and determined its nucleotide and deduced amino acid sequence (embl/genbank accession no. x83218). the encoded polypeptide contains 213 ... | 1995 | 7490082 |
| determination of base specificity of multiple ribonucleases from crude samples. | ribonucleases are widely found on the tissues of living organisms, but the functions of individual ribonucleases are not clear. to facilitate characterization of individual ribonucleases, i have developed a rapid method to separate and identify each ribonuclease from a crude sample by gel electrophoresis instead of by time-consuming purification steps. the ribonucleases in a crude sample are first separated by rna-cast sds-polyacrylamide gel electrophoresis and then eluted from the gel after eth ... | 1994 | 7565655 |
| acaricidal and insecticidal activities of cadina-4,10 (15)-dien-3-one. | the novel assignment of 13c and 1h nmr data for cadina-4,10(15)-dien-3-one obtained from hyptis verticillata is presented. the study revealed that cadina-4,10(15)-dien-3-one possesses chemosterilant activities against the economically important cattle tick, boophilus microplus, and toxic action against adult cylas formicarius the most destructive pest of sweet potato (i pomoea sp.). | 1995 | 7576456 |
| subunit interaction enhances enzyme activity and stability of sweet potato cytosolic cu/zn-superoxide dismutase purified by a his-tagged recombinant protein method. | the coding region of copper/zinc-superoxide dismutase (cu/zn-sod) cdna from sweet potato, ipomoea batatas (l.) lam. cv. tainong 57, was introduced into an expression vector, pet-20b(+). the cu/zn-sod purified by his-tagged technique showed two active forms (dimer and monomer). the amount of proteins of dimer and monomer appeared to be equal, but the activity of dimeric form was seven times higher than that of monomeric form. the enzyme was dissociated into monomer by imidazole buffer above 1.0 m ... | 1995 | 7599315 |
| fractionation and characterization of two forms of peroxidase from oryza sativa. | peroxidase (e.c. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ... | 1995 | 7603969 |
| nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (spfmv-s) genomic rna. | recombinant dna molecules containing cdna of a sweet potato feathery mottle virus severe strain (spfmv-s) rna genome were constructed and the partial nucleotide sequences were determined for three dna inserts, which cover 4-2 kb from the 3'-terminus excluding the poly (a) tail. this region of the genome consists of an open reading frame of 1340 amino acids (a.a.) and a 3'-non-translated region of 224 nucleotides. the protein products expected were 6k2 (53 a.a.), nia (435 a.a.), nib (521 a.a.) an ... | 1995 | 7661699 |
| [epidemiology and control of hookworm infection in fujian province]. | fujian province has been known to be endemic for mixed hookworm infection with ancylostoma duodenale as the predominant species. the infection rate was 50% before 1980. as a result of mass treatment starting from 1980, the infection rate was decreased in most areas. and, infection foci were changed from sweet potato fields to the banana, sweatcane and asparagus fields where the infection rate tended to increase. it is stressed that the current endemic characteristics of hookworm infection should ... | 1994 | 7720205 |
| a large-scale, hospital-based case-control study of risk factors of breast cancer according to menopausal status. | we conducted a large-scale, hospital-based case-control study to evaluate differences and similarities in the risk factors of female breast cancer according to menopausal status. this study is based on a questionnaire survey on life style routinely obtained from outpatients who first visited the aichi cancer center hospital between january 1, 1988 and december 31, 1992. among 36,944 outpatients, 1,186 women with breast cancer detected by histological examination were taken as the case group (607 ... | 1995 | 7730137 |
| nucleotide sequence at the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna. | cdna encoding the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna was cloned and sequenced. the 2324-bp cdna contained an open reading frame (orf) of 2066 bp followed by an 3' non-coding region and a poly(a) region. the orf covered the coat protein and the carboxy terminus of the nuclear inclusion 'b' protein (nib). | 1994 | 7764983 |
| crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato. | sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. its subunit consists of 498 amino acid residues (mr 55,880). it has been crystallized at room temperature using polyethylene glycol 1500 as precipitant. the crystals, growing to dimensions of 0.4 mm x 0.4 mm x 1.0 mm within 2 weeks, belong to the tetragonal space group p4(2)2(1)2 with unit cell dimensions of a = b = 129.63 a and c = 68.42 a. the asymmetric unit contains 1 subunit ... | 1995 | 7777485 |
| a tobacco protein kinase, npk2, has a domain homologous to a domain found in activators of mitogen-activated protein kinases (mapkks). | a cdna (cnpk2) that encodes a protein of 518 amino acids was isolated from a library prepared from poly(a)+ rnas of tobacco cells in suspension culture. the n-terminal half of the predicted npk2 protein is similar in amino acid sequence to the catalytic domains of kinases that activate mitogen-activated protein kinases (designated here mapkks) from various animals and to those of yeast homologs of mapkks. the n-terminal domain of npk2 was produced as a fusion protein in escherichia coli, and the ... | 1995 | 7891653 |
| sweet potato beta-amylase. primary structure and identification of the active-site glutamyl residue. | the complete amino acid sequence of a subunit of sweet potato beta-amylase, a homotetramer, was established by sequence analysis of peptides obtained by digestions with achromobacter protease i and staphylococcus aureus v8 protease and by cyanogen bromide cleavage of the s-carboxymethylated subunit. the subunit of the enzyme is a single polypeptide consisting of 498 amino acid residues. it showed 50-60% identity in the amino acid sequence with those of beta-amylases from soybean and barley, whil ... | 1993 | 8103452 |
| identification of a sweet potato feathery mottle virus isolate from china (spfmv-ch) by the polymerase chain reaction with degenerate primers. | four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. a combined assay of reverse transcription and the polymerase chain reaction utilizing these primers on total rna extracted from ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from china (spfmv-ch), amplified a 1.35 kb and a 830 bp fragment. these amplified fragments were cloned into the bluescript plasmid and sequenced. the comparison of the sequenc ... | 1993 | 8113341 |
| the presequence of a precursor to the delta-subunit of sweet potato mitochondrial f1atpase is not sufficient for the transport of beta-glucuronidase (gus) into mitochondria of tobacco, rice and yeast cells. | a precursor to the delta-subunit of sweet potato mitochondrial f1atpase (pre-f1 delta) has an amino-terminal (n-terminal) presequence of 45 amino acid residues and its n-terminal 18 residues may form an amphiphilic alpha-helix, which is typical of mitochondrial targeting signals [kimura et al. (1990) j. biol. chem. 265: 6079]. fusion genes consisting of sequences that encoded the 25 (dg25), 46 (dg46) and 73 (dg73) n-terminal amino acids from pre-f1 delta fused to the n-terminus of the coding seq ... | 1993 | 8199776 |
| sweet potato feathery mottle potyvirus (c1 isolate) virion and rna purification. | a procedure for the purification of a peruvian isolate (c1) of sweet potato feathery mottle potyvirus (spfmv) and infective rna has been developed. the use of hepes [n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonic acid] buffer containing urea and sodium edta as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from nicotiana benthamiana l. domin. a short rna isolation procedure yielded infectious rna, from which ds cdna of nearly geno ... | 1993 | 8227274 |
| colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants. | various targeting motifs have been identified for plant proteins delivered to the vacuole. for barley (hordeum vulgare) lectin, a typical gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal propeptide. in contrast, the vacuolar targeting information of sporamin, a storage protein from the tuberous roots of the sweet potato (ipomoea batatas), is encoded in an amino-terminal propeptide. both proteins were expressed simultaneously in transgenic ... | 1993 | 8278507 |
| molecular cloning and characterization of cdnas for the gamma- and epsilon-subunits of mitochondrial f1f0 atp synthase from the sweet potato. | mitochondrial f1f0 atp synthases purified from dicotyledonous plants contain six different subunits named alpha, beta, gamma, delta, delta', and epsilon. our previous n-terminal amino acid sequence analyses indicated that the gamma- and epsilon-subunits of the sweet potato mitochondrial f1 correspond to the gamma- and epsilon-subunits of animal mitochondrial f1, respectively (kimura, t., nakamura, k., kajiura, h., hattori, h., nelson, n., and asahi, t. (1989) j. biol. chem. 264, 3183-3186). a cd ... | 1993 | 8349605 |
| lung injury by furanoterpenoids isolated from fusarium solani infected sweet potato, ipomea batatas. | intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from f. solani damaged i. batatas caused pulmonary oedema in albino rats. the elevated broncho alveolar lavage (bal) angiotensin converting enzyme (ace), lactate dehydrogenase (ldh) and protein levels indicated lung damage. the estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions. | 1993 | 8359843 |
| sensory evaluation and nutrient composition of weaning food from pregelatinized maize-sweet potato mixtures. | pregelatinized maize-sweet potato mix, fortified with soybeans and groundnut flours, was evaluated for its acceptability as a weaning food and for nutrient composition. an acceptable product had 14.3, 6.4, 2.4 and 67.4%, respectively, for its protein, fat, ash and carbohydrate contents. values for calcium, phosphorus and and iron contents were 59.6, 187.5 and 2.4 mg/100 g, respectively, while the most predominant fatty acids were linoleic, oleic and palmitic acids. the product, which had a chemi ... | 1993 | 8378272 |
| cloning of the beta-amylase gene from bacillus cereus and characteristics of the primary structure of the enzyme. | the gene encoding the beta-amylase of bacillus cereus bq10-s1 (spoii) was cloned into escherichia coli jm 109. a sequenced dna fragment of 2,001 bp contains the beta-amylase gene. the n-terminal sequences (avngkg mnpdykaylmaplkki), the c-terminal sequences (shtssw), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. the mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 da. the amino acid sequence homology with those ... | 1993 | 8434930 |
| o-glycosylation of a precursor to a sweet potato vacuolar protein, sporamin, expressed in tobacco cells. | sporamin, a vacuolar protein of the sweet potato, is synthesized as a precursor that contains signal peptide and an n-terminal propeptide that functions as a vacuolar targeting determinant. sporamin, when expressed in tobacco cells, migrated as smeared bands on an sds-polyacrylamide gel. the smearing was due to o-glycosylation of the precursor to sporamin. the smeared bands were stained by a glycan-specific stain but no n-glycosylation site was found in the amino acid sequence of the precursor t ... | 1995 | 8580960 |
| replacement of maize with sweet potato (ipomoea batatas l.) tuber meal in broiler diets. | 1. the nutrient composition of sweet potato tuber meal (spm) was similar to that of maize, with the exceptions of crude protein and calcium. the crude protein content was lower (62 v 91 g/kg) and the calcium content was higher (l.9 v 0.l g/kg) in spm. the amino acid composition, in general, favoured spm. 2. trypsin inhibitor activity, and the contents of oxalate and phytate-phosphorus in spm were too low to be of any nutritional concern. 3. the apparent metabolisable energy content of spm was de ... | 1996 | 8833531 |
| effects of oven-drying tubers of two high-protein sweet potato varieties at different temperatures on their feeding value in broilers. | 1. unpeeled tubers from 2 high-protein varieties of sweet potato (white-fleshed bosbok, and orange-fleshed carmel) were chipped and oven-dried at 40, 60, or 80 degrees c, to examine the effects on nutritive value. the dried ground chips were substituted for maize at 500 g/kg and the diets fed to day-old, ross-1 broiler chicks for 3 weeks. 2. compared with carmel, bosbok had a lower crude protein content (90 v. 138 g/kg dm) and trypsin inhibitor activity (tia) (3 v. 5 mg of trypsin inhibited per ... | 1996 | 8833537 |
| application of sweet potato fiber to skin wound in rat. | in order to introduce a suitable drug mixing base or covering for burns or decubital wounds, the usefulness of fiber extracted from the sweet potato was investigated. the healing effect of the fiber was evaluated by examining the extent of reduction in the size of wounds and changes in the quality of wounds in rats. in contrast to the control, the use of the fiber alone as a wound covering material reduced wounded areas by 21.0% at postoperative day 9, 19.5% at day 11, and 18.7% at day 13, and r ... | 1996 | 8839973 |
| isolation, characterisation and cell growth-regulatory properties of kumara (sweet potato) trypsin inhibitors. | locally grown kumara (sweet potato; ipomea batatis) was used as the starting point for the purification of a proteinase inhibitor. the purified inhibitor was highly specific for trypsin, and much less effective as an inhibitor of chymotrypsin. two 22kda variants were present, closely homologous to each other and to sporamin a, but with a single amino acid substitution (proline in place of serine, the second residue in mature sporamin a). one variant had the same n-terminus as sporamin a, whereas ... | 1996 | 8850529 |
| ultraweak luminescence generated by sweet potato and fusarium oxysporum interactions associated with a defense response. | ultraweak luminescence generated by sweet potato and nonpathogenic fusarium oxysporum interactions associated with a defense response was detected by a photoncounting method using ultrahigh-sensitive photodetectors. the time-dependent intensity variation, the spectrum and the two-dimensional imaging of the ultraweak luminescence are indicative of the defense response of the sweet potato to f. oxysporum. the production of ipomeamarone as a phytoalexin means that f. oxysporum induced the defense r ... | 1996 | 8972637 |
| functional activity of sporamin from sweet potato (ipomoea batatas lam.): a tuber storage protein with trypsin inhibitory activity. | sporamin accounts for about 60% to 80% of total soluble protein in sweet potato tubers, and the predicted protein sequence of sporamin shares significant amino acid sequence identity with some kunitz-type trypsin inhibitors. we constructed three recombinant plasmids with cdnas that encode preprosporamin, prosporamin, and sporamin, and these three were expressed in escherichia coli cells as fusion proteins. all three forms of sporamin expressed in e. coli were shown to have strong inhibitory acti ... | 1997 | 9049277 |
| in vitro effects of an acidic by-product feed on bovine teeth. | to evaluate in vitro erosive effects of sweet potato cannery waste (spcw) on bovine incisor enamel. | 1997 | 9140558 |
| the n-terminal propeptide of the precursor to sporamin acts as a vacuole-targeting signal even at the c terminus of the mature part in tobacco cells. | an asparagine-proline-isoleucine-arginine-leucine (npirl) and its related sequences in the n-terminal propeptides (ntpp) of several plant vacuolar proteins, including that of sporamin from sweet potato (spo) function as vacuole-targeting determinants in a manner that is distinct from the vacuole-targeting determinant in the ctpps of other plant vacuolar proteins. when the mutant precursor to sporamin, spo-ntpp (in which ntpp was moved to the c terminus of the mature part), was expressed in tobac ... | 1997 | 9232873 |
| complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus. | the 5'-terminal genomic region (8597 nt) of little cherry virus (lchv), a mealybug-borne closterovirus, was cloned from double-stranded rna, and its sequence determined to complete the 16934 nt sequence of the monopartite lchv rna genome. in the 5' to 3' direction, the sequence encompasses orf 1a, encoding the conserved replicative domains of methyltransferase and helicase, and orf 1b, encoding rna polymerase. orfs 1a and 1b partially overlap (in o/+1 configuration), and the lchv replicase is pr ... | 1997 | 9267008 |
| utilization of fodder yeast and agro-industrial by-products in production of spores and biologically - active endotoxins from bacillus thuringiensis. | a number of newly-devised fermentation media were evaluated with respect to their ability to support sporulation and biosynthesis of endotoxins by strains of bacillus thuringiensis that are biologically active against spodoptera littoralis, heliothis armigera, and spodoptera exigua. fodder yeast from dried cells of saccharomyces cerevisiae could be used as a complete mono-component medium for production of highly active spore-delta-endotoxin complexes from b. thur., vars. entomocidus, kurstaki a ... | 1983 | 6666415 |
| [host plants of bemisia tabaci (homoptera: aleyrodidae) in cuba]. | the sweet potato white fly, bemisia tabaci, is an important pest of tomatoes and beans, among other crops, which transmits viral diseases. since the second quarter of 1989 a significant population increase of this pest has been noted in several cultivated plants. from 1989 to 1992, a survey was done throughout the country, chiefly in vegetable and bean-producing areas. they occur in 119 species (42 families), a great increase over the previous record of four species. worldwide, this report repre ... | 1997 | 9404518 |
| proximate composition and selected functional properties of african breadfruit and sweet potato flour blends. | full-fat african breadfruit flour was used to replace 30, 40, 50, 60 and 70% of sweet potato flour. the chemical composition and functional properties of composite flours showed that they contain more protein, fat, and ash and less carbohydrate than sweet potato flour. with increasing level of supplementation of breadfruit, ash, protein and fat contents increased while carbohydrate decreased. the composite flours possessed higher water absorption than sweet potato flour. the water absorption cap ... | 1997 | 9498694 |
| effects of gamma irradiation on the flavor composition of food commodities. | fifteen food products including potato, sweet potato, shallot, onion, garlic, ginger, papaya, mango, rice, tobacco, small red bean, mungbean, soybean, wheat, flour and spices have been approved for irradiation by the national health administration in taiwan. market tests (wu et al., 1996) provided strong proof that taiwanese consumers would accept irradiated foods. however, researchers in the food industry are concerned about the possibility of chemical changes, especially in volatile compositio ... | 1998 | 9598207 |
| identification and production of local carotene-rich foods to combat vitamin a malnutrition. | objectives: to address, with respect to improvement of human vitamin a status by dietary approaches, the three theoretical postulates that: 1) the most practical and economical manner to increase the amount of dietary vitamin a available to low-income persons in low-income nations is through plant sources of provitamin a carotenoids; 2) there will be constraints and limitation to the efficiency of a given intervention approach related to behavioural, cultural, biological and botanical considerat ... | 1997 | 9598793 |
| an antigenic protein gene of a phytoplasma associated with sweet potato witches' broom. | a gene encoding the major antigenic protein of phytoplasma associated with sweet potato witches' broom (spwb) was cloned and analysed by screening the genomic library of spwb phytoplasma with monoclonal antibodies for spwb phytoplasma. the entire predicted structural gene encoded an antigenic protein composed of 172 amino acids with a computed molecular mass of 19.15 kda and a pl value of 9.78. the -10 region of the promoter and the terminator region of the gene were identified and found to be s ... | 1998 | 9611800 |
| production of human lactoferrin in transgenic tobacco plants. | production and characterization of human lactoferrin (hlf) in transgenic tobacco is reported. we have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encoding cdna. n-terminal sequences of rhlf purified from tobacco were identical to lf from human milk for both constructs. the tobacco rhlf presents a molecular mass closely identical to native protein. overall sugar composition ... | 1998 | 9631525 |
| complete nucleotide sequence and genome organization of sweet potato feathery mottle virus (s strain) genomic rna: the large coding region of the p1 gene. | the complete nucleotide sequence of a sweet potato feathery mottle virus severe strain (spfmv-s) genomic rna was determined from overlapping cdna clones and by directly sequencing viral rna. the viral rna genome is 10,820 nucleotides long, excluding the poly(a) tail and contains one open reading frame (orf) starting at nucleotide 118 and ending at 10,599, potentially encoding a polyprotein of 3,493 amino acids (mr 393,800). the orf was followed by a 3' untranslated region of 221 nucleotides. the ... | 1997 | 9672618 |
| sequencing and characterization of the coat protein and 3' non-coding region of a new sweet potato potyvirus. | a cdna library was constructed from viral genomic rna purified from sweet potato plants affected by "sweet potato chlorotic dwarf disease" in an attempt to clarify the etiology of this viral complex in argentina. by sequence analysis, some of the obtained clones were found to belong to sweet potato feathery mottle potyvirus (spfmv), to a closterovirus and to a new potyvirus. a cdna clone of 1,103 bp representing the coat protein cistron and 3' non-coding region of the newly identified potyvirus ... | 1997 | 9672624 |
| rna extraction from virus-diseased sweet potato for reverse transcriptase polymerase chain reaction analysis. | sweet potato virus disease (spvd), which causes severe crop losses in africa, is caused by a complex of sweet potato feathery mottle potyvirus (spfmv) and sweet potato chlorotic stunt crinivirus (spcsv). extraction of pure rna (for diagnosis by reverse transcriptase polymerase chain reaction [rt-pcr] methods) from diseased sweet potato proved to be difficult using five different total-rna extraction procedures: (a) that of chomczynski and sacchi (1) (b) an adaptation of lodhi et al., (2) and thr ... | 1998 | 9819817 |
| dried green leaf powders of jew's mellow (corchorus), persimmon (diosphyros kaki) and sweet potato (ipomoea batatas poir) lower hepatic cholesterol concentration and increase fecal bile acid excretion in rats fed a cholesterol-free diet. | a cholesterol-free diet containing dried powder of jew's mellow leaves, persimmon leaves or sweet potato leaves respectively at 5% level as dietary fiber was fed to male sprague-dawley rats for about one month. the experiment was conducted twice except for sweet potato leaves. in the groups fed the diet mixed with powders of any of the three different dried green leaves, the hepatic cholesterol concentration significantly decreased. such lowering was not observed in serum cholesterol concentrati ... | 1998 | 9839835 |
| sequence analysis of the coat protein and 3'-noncoding region for korean and other strains of sweet potato feathery mottle virus. | the 3'-terminal regions of the genomic rnas of two korean isolates of sweet potato feathery mottle potyvirus (spfmv) were cloned and their nucleotide sequences of full-length coat protein (cp) gene and 3' noncoding region (ncr) were determined. the cp of the two korean isolates contained 315 amino acid residues. the cp cistron sequences of the korean isolates exhibit 72.7% to 98.7% nucleotide sequence identity and 79.9% to 99.0% amino acid identity when compared with those of 8 other known spfmv ... | 1998 | 9895134 |
| l-galactono-gamma-lactone dehydrogenase from sweet potato: purification and cdna sequence analysis. | l-galactono-gamma-lactone dehydrogenase (ec 1.3.2.3, gldhase) was partially purified from mitochondria of sweet potato tuberous roots over 600-fold on a specific activity basis, followed by purification of the enzyme protein of 56 kda by a preparative sds-page. the absorption spectrum of the hydroxylapatite column-purified gldh-ase showed peaks at 448 and 373 nm, suggesting the presence of flavin as a prosthetic group. the activity of gldh-ase was inhibited by lycorine, an alkaloid which inhibit ... | 1998 | 10050319 |
| antimutagenicity of sweetpotato (ipomoea batatas) roots. | antimutagenicity of the water extracts prepared from the storage roots of four varieties of sweetpotato with different flesh colors was investigated using salmonella typhimurium ta 98. the extract from the whole roots of the purple-colored ayamurasaki variety effectively decreased the reverse mutation induced not only by trp-p-1, trp-p-2, iq, b[a]p, and 4-nqo but also by dimethyl sulfoxide extracts of grilled beef. comparison of the inhibitory activity of the extracts from the normal ayamurasaki ... | 1999 | 10227139 |
| isolation of the gene encoding an immunodominant membrane protein of the apple proliferation phytoplasma, and expression and characterization of the gene product. | an immunodominant membrane protein (imp) of the apple proliferation (ap) phytoplasma was detected in preparations from ap-diseased periwinkle plants using monoclonal and polyclonal antibodies to the ap agent. following isolation from western blots and partial sequencing, degenerate oligonucleotides derived from the imp sequence were used as probes to identify a dna fragment containing the orf encoding the imp. complete sequencing and subsequent analysis of the cloned dna fragment revealed the pr ... | 1999 | 10463160 |
| expression patterns of two genes for the delta-subunit of mitochondrial f1-atp synthase from sweet potato in transgenic tobacco plants and cells. | two nuclear genes, f1 delta-1 and f1 delta-2, coding for the delta-subunit of mitochondrial f1-atp synthase, which corresponds to oligomycin-sensitivity conferring protein in animal and yeast mitochondria, were isolated from sweet potato. the gene for the delta-subunit was composed of 6 exons and these two genes shared high sequence similarities to each other not only in exons but also in introns and in the 5'-upstream regions. however, the 5'-upstream regions of f1 delta-1 and f1 delta-2 were d ... | 1999 | 10555307 |
| post-translational maturation of natural and drug-induced missorted phytohemagglutinin. | the bean lectin phytohemagglutinin (pha) was expressed in transgenic suspension-cultured by-2 tobacco cells simultaneously with another recombinant vacuolar protein, the sweet potato sporamin. in contrast to previous observations in different transgenic plant systems when expressed in by-2 tobacco cells, phytohemagglutinin is mostly but not exclusively targeted to the vacuole. indeed, a small amount of recombinant phytohemagglutinin is secreted into the culture medium of tobacco cells. furthermo ... | 1999 | 10580285 |
| performance of broiler chickens fed sweet potato meal (ipomoea batatas l.) diets. | day-old lohman broiler chicks (n = 120) were fed on five starting diets for 4 weeks in groups of 24 birds. the starting diets contained 0%, 9%, 18%, 27% and 36% sweet potato tuber as a replacement for maize. from the fifth week, the 120 birds were tested in groups of 30 on four finishing diets containing 0%, 15%, 30% and 45% sweet potato tuber as a replacement for maize. the carcass quality was significantly (p < 0.05) improved due to a significant (p < 0.05) reduction of abdominal fat in the bi ... | 1999 | 10599133 |
| the n-terminal propeptide and the c terminus of the precursor to 20-kilo-dalton potato tuber protein can function as different types of vacuolar sorting signals. | two types of vacuolar sorting signals (vsss), an asparagine-proline-isoleucine-arginine-leucine (npirl)-related vss in the n-terminal propeptides (ntpps) and a c-terminal vss in the c-terminal propeptides (ctpps), function differently in plant cells. a precursor to a 20-kda protein of potato tuber (pt20) contains two npirl-related sequences, npinl in a short ntpp and npldv close to the c terminus of the precursor. we made mutant forms of sweet potato sporamin (spo), npt20-spo, in which the n-ter ... | 1999 | 10635117 |
| purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, agrius convolvuli. | three chitin-binding proteins (cbps: cbp9, cbp15, cbp66) were identified from the larval hemolymph of sweet potato hornworm, agrius convolvuli. two (cbp9 and cbp15) of them have been isolated and purified by gel filtration (superdex hr 75), cation-exchange chromatography (mono s), and reverse-phase chromatography (murpc pc 2.1/3). in experiments to detect cbps in hemolymph, we examined whether ionic strength and existence of bovine serum albumin in the incubation solution influenced binding affi ... | 1999 | 10665375 |
| antidiabetic activity of white skinned sweet potato (ipomoea batatas l.) in obese zucker fatty rats. | antidiabetic effects of white skinned sweet potato (ipomoea batatas l.) (wssp) and troglitazone, an insulin sensitizer, were investigated. hyperinsulinemia in zucker fatty rats was reduced by 23%, 26%, 60% and 50%, respectively, 3, 4, 6 and 8 weeks after starting the oral administration of wssp. similar results were obtained with troglitazone. in the glucose tolerance test after 7 weeks of treatment, increases in blood glucose levels after glucose loading were inhibited by the administration of ... | 2000 | 10706405 |