| polyamino acid induced aphid transmission of plant viruses. | aphids transmitted poly-l-ornithine (plo)-treated tobacco mosaic virus (tmv) when given acquistion and inoculation access periods as brief as 30 s and 2 min, respectively; the ability to transmit was lost within 90 min. aphids without claws were able to transmit the virus. transmission thus seems similar to that of nonpersistent viruses. the ratio of virus to polyamino acid, as well as the kcl concentration, markedly affected transmission. transmission was best from mixtures which contained 250 ... | 1975 | 1467 |
| removal of phosphate groups from casein with potato acid phosphatase. | potato acid phosphatase (ec 3.1.3.2) was used to remove the eight phosphate groups from alphas1-casein. unlike most acid phosphatases, which are active at ph 6.0 or below, potato acid phosphatase can catalyze the dephosphorylation of alphas1-casein at ph 7.0. although phosphate inhibition is considerable (k1=0.42 mm phosphate), the phosphate ions produced by the dephosphorylation of casein can be removed by dialysis, allowing the reaction to go to completion. the dephosphorylated alphas1-casein ... | 1976 | 4132 |
| comparison of antibiotic-amended potato dextrose agar and acidified potato dextrose agar as growth substrates for fungi. | fifteen fungal species, all isolated from food, were compared for their growth abilities on potato dextrose agar acidified to ph 3.5, and on nonacidified potato dextrose agar amended with 40 ppm chlortetracycline hydrochloride. comparisons were made at 16, 21, 26, 32, and 37 degrees c. of the 15 species, only penicillium expansum exhibited better growth on the acidified medium than on the nonacidified antibiotic medium, while 9 species grew better on the nonacidified antibiotic medium. five spec ... | 1976 | 5395 |
| a ph-dependent conformational change in the coat protein subunits from potato virus x. | both the circular dichroism and fluorescence spectra of the dissociated coat protein subunits from potato virus x changed substantially over the ph range 8 to 4, irreversible changes resulted below ph 4, with tyrosyl and tryptophanyl residues affected most. the titration curves show a pka of about 5.6 and do not require cooperative interactions between the coat protein subunits, thus they are in marked contrast to titrations of tobacco mosaic virus a-protein. the spectra of the intact virus were ... | 1976 | 10980 |
| assembly of flexuous plant viruses and their proteins. | recent experiments on the disassembly and assembly of some flexuous plant viruses and their proteins are described. the properties of reconstituted potato virus x and those of assembled potato virus y protein are considered as well as the suitability of other flexuous viruses for reconstitution studies. | 1976 | 13429 |
| dog renal kallikrein: purification and some properties. | the contents of kallikrein ec 3.4.21.8 in the kidneys of various animals were estimated and the activity was found to be most potent in dogs. the dog renal kallikrein (drk) was located mainly in the kidney cortex. following the activation of a dog kidney cortex homogenate with acetone, kallikrein was purified about 2,000-fold with an overall yield of 18% by diethylaminoethyl (deae)-cellulose adsorption, acetone fractionation, and chromatography on sephadex g-75 and deae-sephadex a-50. the final ... | 1976 | 14121 |
| production of schardinger beta-dextrin by soluble and immobilized cyclodextrin glycosyltransferase of an alkalophilic bacillus sp. | succinylated cyclodextrin glycosyltransferase (ec 3.2.1.19) of an alkalophilic bacillus sp. was adsorbed on a vinylpyridine copolymer. the enzyme had about 25% of the activity of soluble enzyme added. no increase of ph or thermal stability of the enzyme was observed by the adsorption, whereas optimum temperature for the enzyme action was shifted from 50 to 55 degrees c. the enzyme converted starch to cyclodextrine without significant loss of activity under the conditions of 4 times reusing of 6 ... | 1977 | 14746 |
| purification and some properties of an extracellular alpha-amylase from bacteroides amylophilus. | a medium was developed to obtain maximum yields of extracellular amylase from bacteroides amylophilus 70. crude enzyme preparation, obtained by ammonium sulfate precipitation of cell-free broth, contained six amylolytic isoenzymes that were detected by isoelectric focusing and polyacrylamide gel electrophoresis. one of these amylases was purified by diethylaminoethyl-sephadex a-50 ion-exchange chromatography and sephadex g-200 gel filtration techniques. some properties of the purified extracell ... | 1977 | 14926 |
| the fermentative production of acetone-butanol by clostridium acetobutylicum. | fourteen different media were used in the fermentative production of acetone-butanol. the highest total yields were achieved in medium i. potato starch and soluble starch were suitable as carbon sources. the best concentrations of potato starch and soluble starch were 500.0 and 10.0 g/l, respectively. peptone was the most favourable nitrogen source. the best concentration of peptone was 4.0 g/l. calcium carbonate in 3.6 g/l acted as buffering agent in the fermentation process. the best initial p ... | 1976 | 16418 |
| effect of external environmental factors on the morphology of spiroplasma citri. | spiroplasma citri was examined by electron microscopy for morphological changes when maintained under a variety of conditions. pplo serum fraction maintained spiral and helical morphology of s. citri at ph values of 8.0, 7.5, and 7.0, but only partially at ph 6.0 and 5.0. the absence of pplo serum fraction resulted in round, deteriorated cells at all ph values tested. bovine serum albumin (bsa), phytone, soluble starch, potato starch, spermine, lipid-extracted pplo serum fraction, and lipid-extr ... | 1978 | 24042 |
| antiphytoviral activity of 2,4 dioxo hexahydro triazine. | three treatments with 2,4 dioxo hexahydro triazine (dht) significantly reduced the concentration of potato virus x (pvx) in systemically infected tobacco plants. in hypersensitive plants dht caused a reduction in the number of local lesions produced by pvx. in systemic and hypersensitive hosts, treatment with dht resulted in a more or less marked reduction in the concentration of, and in the number of local lesions caused by, potato virus y (pvy), potato virus a (pva), tobacco mosaic virus (tmv) ... | 1979 | 42301 |
| reconstruction of potato virus x in vitro. ii. characterization of the reconstituted product. | | 1975 | 53939 |
| synthesis of dna transcripts of potato spindle tuber viroid. | | 1977 | 67055 |
| nutritive role of different carbohydrates for the growth and survial of oryzaephilus mercator fauvel (coleoptera). | recent investigations on the nutritive role of different carbohydrates for the survival and growth of oryzaephilus mercator show that fructose, glucose, dextrin and glycogen are of high nutritive value, fructose being even better than natural control diet (wheat + 5% yeast). maltose, soluble starch, mannitol and sorbitol, although support some growth, are not of as good nutritive value as control diet. all pentoses, sorbose, galactose, lactose, raffinose, potato starch, inulin and dulcitol are o ... | 1976 | 71029 |
| statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins a, b, and c. | the objective of these studies was to set up a reliable radioimmunoassay (ria) for staphylococcal enterotoxins a, b, and c (sea, seb, and sec) in a food system. significant differences (95% confidence limits) were obtained between the 0- and 1-ng/ml enterotoxin standards, so the sensitivity of the rias was 1 ng/ml. polystyrene tubes coated with anti-seb and stored at 4 degrees c were unstable. the percentage of iodinated seb bound to these tubes decreased at a rate of 0.33%/day, in contrast to t ... | 1977 | 74235 |
| reverse transcription of turnip yellow mosaic virus rna primed with calf-thymus dna hydrolysate: characterization of the purified cdna product. | complementary dna was transcribed from turnip yellow mosaic virus rna, using the method of taylor et al. (1). the purified cdna thus obtained sedimented between 2 and 4 s and was a mostly uniform transcript of template rna. it hybridized with a sharp transition to homologous tymv-rna (crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the rnas of two other tymoviruses, namely andean potato latent virus and eggplant mosaic virus. | 1978 | 82938 |
| the enzymatic hydrolysis of the phosphate ester bond in some thionucleotides. | we prepared the 5'- and 3'-o-phosphorothioate esters of the antitumor agent o2 : 2'-anhydro-1-beta-d-arabinosylcytosine. we also included in this study esters of 2'-thio-2'-deoxycytidine, namely, 2'-s-dcyd-2' : 3'-p, 2'-s-dcyd-2'-p, and 2'-s-dcyd-3'-p, along with natural nucleotides. these compounds were subjected to the action of escherichia coli alkaline phosphatase, potato acid phosphatase, and bovine pancreatic ribonuclease a. the data were analyzed by lineweaver-burk plots to obtain km and ... | 1979 | 84687 |
| selective vital staining of companion cells of potato tuber and parsnip root with neutral red. | when staining the internal phloem region of a potato tuber with the vital stain neutral red, it was observed that files of elongated cells of narrow diameter were heavily stained and were easily distinguishable from the more isodiametric parenchyma cells, many of which did not stain with neutral red. the elongated cells were identified as companion cells by locating the adjacent sieve-tube members through counterstaining with aniline blue and reviewing under violet light. of a number of other pl ... | 1979 | 91243 |
| study of the interaction between phosphorylase and hydrophobic groups by means of affinity electrophoresis. | a homologous series of water-soluble alkyl-dextrans varying in the length of their hydrocarbon side-chain [-nh-(ch2)n-ch3; n = 1-5] were synthesized. when alkyl-dextrans were entrapped in polyacrylamide gel, the electrophoretic mobility of phosphorylase was retarded by hydrophobic interaction between phosphorylase and the immobilized alkyl groups. the dissociation constants of rabbit brain phosphorylase, rabbit skeletal muscle phosphorylase a and b and potato glycogen and starch phosphorylases w ... | 1979 | 94915 |
| [potato leafroll virus: purification, serologic detection and quantitative analysis in plants]. | the potato leafroll virus has been purified from potato. an antiserum obtained allows the use of the enzyme linked-immunosorbent assay, a direct means to detect the plrv in the plant and to assess its real concentration. | 1978 | 96980 |
| production and chemical studies of protease from bacillus subtilis (sh-6) egyptian strain. | bacillus subtilis (sh-6) egyptian strain, isolated from hides, gave the highest protease activity. luxurious growth and protease production were obtained by the use of a medium containing 8% of potato starch, 0,1 m of ammonium phosphate as carbon and nitrogen sources. results indicate that borate buffer exerted a deleterious effect on the protease production. comparing citrate and phosphate buffers, it was found that citrate gave lower protease activity than phosphate. there is a positive respon ... | 1978 | 98932 |
| [economic evaluation of the method of gradual formation of groups of dairy cows from primiparae free of infectious mastitis]. | employing the turnover of a dairy-cow herd with primiparas to control infectious mastitis is beneficial because young dairy cows, as a rule free of inflammations of the mammary gland, are not included among dairy cows already infected or suspected of being infected and so it is possible to form groups of dairy cows free of mastitis. for this intention an agricultural enterprise was chosen in potato production region i. in the given period 316 to 336 dairy cows of bohemian spotted breed were foll ... | 1978 | 102068 |
| [use of protein-free potato juice concentrates in the production of tetracycline]. | the study on the use of potato juice concentrate in tetracycline production showed that the tetracycline-producing strain 3-nk of actinomyces aureofaciens grown on seed and fermentation media containing 2--2.5 and 0.35--0.45 per cent (by dry substance) of the concentrate produced the same antibiotic yield as that on the media with corn steep liquor. | 1978 | 102242 |
| [discovery of viroids and possible relationship with human and veterinary medicine]. | the researches of diener and co-workers and those of the semancik's group, have recently established that some plant diseases, such as potato spindle tuber, citrus exocortis disease and chrysanthemum stunt, are caused by a new class of pathogens, named viroids. these are the smallest known agents (they are smaller than viruses) having a molecular weight of ca 10(5) daltons, and composed of a highly structured rna, rich in guanine-cytosine base pairs without a capsid. little is known about the or ... | 1975 | 132907 |
| [experimental investigations on ultraweak photonemission form biological systems (author's transl)]. | measurements on untreated yeast cells, potato- and cucumber seedlings have confirmed the existence of photon emission from biological systems known as "ultraweak luminescence". the intensity is of the order of 10(2) counts per second, and the spectral distrubtion shows a maximum near 550 nm. the dependence of th photon emission on certain chemicals has been investigated. some chemicals, as for instance acetone, intensify the photon emission from cucumber seedlings without essential change of the ... | 1976 | 138296 |
| [group-allergic reaction spectrum and sensitization potency of benzylisothiocyanate]. | allergenicity of benzylisothiocyanate and group-allergic cross reactions. comparative patch-test series made in pateints suffering from contact dermatitis induced by sulbentin (dibenzthion)-antimycotics and in guinea pigs, experimentally sensitized to benzylisothiocyanate (bitc), showed: bitc is a potent contact sensitizer and the real hapten in cases of sulbentin-allergy. of special importance in this molecule is the benzyl-group. neither 4,4-diphenylmethandiisocyanate and isophorondiisocyanate ... | 1976 | 138417 |
| activation of chromatin-bound dna-dependent rna polymerase (e.c. 2.7.7.6.) in plant storage tissue slices. | the synthesis of rna by chromatin-bound rna polymerase (e.c. 2.7.7.6.) from white potato tubers proceeds at a low rate, which is enhanced after slicing the tissue, however. concomitantly dna template availability as measured with saturating amounts of escherichia coli polymerase is diminished drastically. nearest neighbor frequency analysis proved that the rna synthesized on chromatin of intact tubers is different from that synthesized on chromatin of sliced tissue. the rna polymerase of white p ... | 1977 | 141805 |
| improved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme a reductase. | a simple and rapid mixed-phase method for the quantitative assay of 3-hydroxy-3-methylglutaryl (hmg)-coa reductase and a procedure for the efficient reactivation of mg-atp-inactivated microsomal hmg-coa reductase by potato acid phosphatase are described. the mixed-phase assay entails the direct addition of the acidified, deproteinized incubation mixture to a toluene-based scintillation fluor. the enzymatic reaction product [3h]-mevalonolactone partitions into the toluene while unreacted 3h-label ... | 1979 | 158623 |
| influence of starch source on sporulation and enterotoxin production by clostridium perfringens type a. | of 16 different starch preparations tested, clostridium perfringes nctc 8798 yielded maximum sporulation and enterotoxin formation when icn-soluble starch was included in duncan and strong sporulation medium. in general soluble starches were better than potato, corn, or arrowroot starch with regard to these two parameters. | 1976 | 180885 |
| epr studies of higher plant mitochondria. i ubisemiquinone and its relation to alternative respiratory oxidations. | an epr investigation of the region of the higher plant respiratory chain involving ubiquinone and center s-3 of succinate dehydrogenase is reported. at temperatures close to those of liquid helium, first derivative spectra corresponding to center s-3 (gmax = 2.017) and a signal split around g = 2.00 (major features of peaks and troughs at g values of 2.045, 2.03, 1.985, 1.97 and 1.96) were observed in mung bean (phaseolus aureus), arum maculatum spadix, sauromatum guttatum spadix and tulip bulb ... | 1977 | 202304 |
| polymyxin resistance in agrobacterium tumefaciens and its effect on crown gall tumor induction. | polymyxin-resistant (pblr) mutants of agrobacterium tumefaciens a6, b6, and b6m were isolated from polymyxin-sensitive (pbls) parent strains in a defined medium containing 600 microgram of polymyxin b sulfate per millilitre. the weight and number of tumors induced by pblr mutants on a variety of host plants such as carrot, potato, and pinto bean were 45--75% less than those induced by pbls wild types. the crude cell envelopes (cce) prepared from both pbls and pblr bacteria were inhibitory for tu ... | 1979 | 219950 |
| l-phenylalanine ammonia-lyase (maize, potato, and rhodotorula glutinis). studies of the prosthetic group with nitromethane. | highly purified enzyme (ec 4.1.3.5) from rhodotorula glutinis was shown by sodium dodecyl sulfate gel electrophoresis to have subunits which if not identical are closely similar in molecular weight. like the enzyme from maize and potato [havir, e. a., and hanson, k. r. (1973), biochemistry 12, 1583] it is a tetramer of molecular weight similar to 4 times 83,000. enzyme from all three sources inactivated and labeled at the active site with 14-ch3no2 gave on hcl hydrolysis 14-co2, h-14-co2h, d- an ... | 1975 | 235961 |
| photooxidation of alpha-glucan phosphorylases from rabbit muscle and potato tubers. | photooxidation of alpha-glucan phosphorylases from rabbit muscle and potato tubers in the presence of rose bengal leads to a rapid loss of enzymatic activity which follows first-order kinetics. the process is ph dependent, being more rapid at higher ph. the inactivation is closely related to the destruction of histidine residues in the enzyme. it is suggested that histidine residues are largely responsible for the loss of enzymatic activity in the photooxidation. the inactivation of potato phosp ... | 1975 | 241401 |
| [effect of potato starch on the level of volatile fatty acids and ammonia in sheep's rumen]. | two experiments were performed on adult sheep, which were given intraruminally potato starch in a single dose of 1500 g and in gradually increasing doses. the effect of starch on the level of volatile fatty acids, ammonia and the ph value was studied. in the rumen content, the level of ph slightly decreased and that of volatile fatty acids increased. ammonia level decreased after starch administration. no lactic acid was found in the rumen content. | 1975 | 241977 |
| separation and infectivity of circular and linear forms of potato spindle tuber viroid. | potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. one fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. the purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. the length distributions of denatured circular and linear molecules were determined. both circular and linear molecules were found to be ... | 1977 | 269437 |
| [cyclodextrin glucanotransferase from klebsiella pneumoniae. 1. formation, purification and properties of the enzyme from klebsiella pneumoniae m 5 al (author's transl)]. | 1. the strain m 5 al of klebsiella pneumoniae grows excellently with starches. we were able to show that besides the pullulanase associated with the external membrane of the cells the bacterium produces an inducible, extracellular cyclodextrin glucanotransferase [1,4-alpha-d-glucan-4-alpha-(1,4-alpha-glucano)-transferase (cyclising) (ec 2.4.1.19)]. potato starch and cyclohexaamylose or cycloheptaamylose were found to be the best "inducing" carbon sources for the synthesis of the enzyme. when the ... | 1977 | 319771 |
| maleic hydrazide: should the delaney amendment apply to its use? | the chemistry, metabolism, toxicology, mutagenicity, and carcinogenicity of maleic hydrazide have been reviewed. there is little doubt that this chemical is a mutagen and a carcinogen in cell cultures and animals, but no evidence is available on human carcinogenicity regardless of population exposure in manufacturing, agriculture, and the food chain (i.e., potato chips). an epidemiology survey should be conducted to ascertain possible human carcinogenicity in these populations. a long-term inges ... | 1977 | 325222 |
| serial subculture of bcg on solid and liquid media. | to follow an earlier study of the effects on bcg of deep subculture in liquid media, transfers on the surface of löwenstein-jensen and sauton's media have been made. when a vaccine preparation that yields on culture 99+% of spreading-type colonies, with a small minority of non-spreading forms, is transferred on löwenstein-jensen medium, the minority population can be readily selected out. growth of bcg on this medium is not homogeneous, and the selection of either the minority or majority popula ... | 1979 | 382555 |
| effects of differences in amount and kind of dietary carbohydrate on plasma glucose and insulin responses in man. | the effect of variations in kind and amount of dietary carbohydrate on plasma glucose and insulin responses was studied in normal subjects and in patients with chemical diabetes. plasma glucose and insulin responses fell when the proportion of total calories given as carbohydrate were decreased by 15% (coincidental with a comparable increase in dietary fat). associated with the lower plasma glucose and insulin concentrations was a fall in fasting and postprandial triglyceride levels. plasma gluc ... | 1979 | 389023 |
| improved detection of coliforms and escherichia coli in foods by a membrane filter method. | analytical procedures based on filtration of homogenates through membrane filters, and particularly hydrophobic grid-membrane filters (hgmf), offer definite improvements in the enumeration of escherichia coli and coliforms in foods. whereas the counted specimen in pour plates may not usually be greater than 0.1 g, up to 1.0 g of ground beef, green beans, potato, cod, strawberries, or grapes could be filtered and counted on hgmf. greatly improved limit of detection, reduced interference by noncol ... | 1979 | 394679 |
| teratogenicity studies on late blighted potatoes in nonhuman primates (macaca mulatta and saguinus labiatus). | female rhesus monkeys and marmosets were fed a diet containing blighted potatoes (phytophthora infestans) at a level of 10g/kg per day for at least two weeks prior to breeding and six weeks following conception in order to gain additional information on the association of blighted potatoes and the development of anencephaly and spina bifida in primate species. there was an absence of either of these neural-tube defects in 32 rhesus and 14 marmoset infants whose mothers had received a blighted po ... | 1977 | 402704 |
| [comparative balance study of the value of amino acids for humans after oral and parenteral input]. | the biological value (bv) of an amino acid mixture, according to the potato-egg principle (ke), was determined in the case of oral and parenteral input with three healthy adult volunteers in a long-term complete test of metabolic balance: ke-amino acid pattern with oral input, bv=134.4; ke-amino acid pattern with parenteral input, bv= 131.4; egg protein (reference) with oral input, bv=100. the long-term metabolic balance experiment with human subjects under criteria of compensated nitrogen balan ... | 1978 | 413070 |
| studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease a and ribonuclease t1, and modification with bisulfite. | potato spindle tuber viroid (pstv), a small infectios rna, has been completely digested with rnase t1 and rnase a, and the resulting oligonucleotides have been sequenced using 5'-terminal 32p-labelling with gamma-32p atp and t4 polynucleotide kinase, fingerprinting and controlled nuclease p1 digestion. modified nucleotides have not been detected in 5'-positions of these oligonucleotides. pstv consists of about 359 nucleotides and contains a remarkable stretch of 18 purines, mainly adenosines; th ... | 1978 | 418383 |
| [biological value of new canned baby foods stabilized with various types of starch]. | the nature of influence produced by native and modified starches on the biological value of new baby food homogenized canned meat of the "kroshka" type was studied. experiments on animals have found the stabilization of the structure of approbated canned goods modified with phosphate starches, such as maze, amylopectin and potato (ty 18 rsfsr 279-73) ones, causes lowering of the biological value of the product by comparison with analogous native starches. | 1979 | 419729 |
| phenylalanine ammonia-lyase: enzymic conversion of 3-(1,4-cyclohexadienyl)-l-alanine to trans-3-(1,4-cyclohexadienyl)acrylic acid. | the phenylalanine analogue 3-(1,4-cyclohexadienyl)-l-alanine is converted to the hitherto unknown cinnamate analogue trans-3-(1,4-cyclohexadienyl)acrylic acid by l-phenylalanine ammonia-lyase (ec 4.3.1.5) from maize, potato, or rhodotorula glutinis. the structure assigned to the product is confirmed by its 1h nuclear magnetic resonance spectrum and by the chemical synthesis to be described in a subsequent paper. on comparing the above substrate analogue with l-phenylalanine, the km was lowered o ... | 1979 | 427124 |
| evidence for a single infectious species of potato spindle tuber viroid. | potato spindle tuber viroid (pstv) has been separated into two electrophoretic species on polyacrylamide gels containing 8 m urea at 60 degrees. only the slower-migrating electrophoretic form was infectious and is presumably the circular form of the molecule. both forms had similar base compositions with a 55-58% gc content, similar to the citrus exocortis viroid. labeling studies in vivo suggest that the viroid is not methylated. | 1979 | 429145 |
| crayfish carboxypeptidase. affinity chromatography, characterization and amino-terminal sequence. | in an effort to trace the evolutionary history of the pancreatic metalloexopeptidases, carboxypeptidase has been isolated from the cardia of the crayfish astacus fluviatilis. the isolation procedure included affinity chromatography on a column of potato carboxypeptidase inhibitor covalently linked to sepharose. approximately 25 mg of pure enzyme can be obtained by the present procedure from 50 ml of cardia fluid. the pure enzyme resembles bovine carboxypeptidase b in specificity and is inhibited ... | 1979 | 436840 |
| rna in potato leafroll virus. | | 1979 | 446731 |
| [activity, isoenzyme composition, thermostability and molecular weight of peroxidase from intact and virus infected tobacco plant leaves]. | the enzyme peroxidase was isolated from the leaves of the tobacco plant xanthi (intact and infected with weakly (xy) and highly (xt) pathogenic strains of potato x-virus) and partially purified. the original extract (the 30,000 g supernatant) was purified by ammonium sulfate at 30--80% of saturation and by gel filtration through sephadex g-25 and g-100 in 0.05 m tris-hcl buffer, ph 7.4 containing 17% sucrose. disc electrophoresis revealed that both intact and infected plants contain 10 isoperoxi ... | 1979 | 465587 |
| [dioxohexahydrotriazine, a new synthetic antiphytoviral compound (author's transl)]. | this paper reports the first results of studies made to determine the antiviral activity of dioxo hexahydro triazine, a newly developed antiphytoviral compound. after treatment with dioxo hexahydro triazine, the concentration of helical potato virus x in leaves of nicotiana tabacum "samsun" was found to be considerably reduced in both the serological test and local lesion test conducted on nicotiana glutinosa at temperatures of 14 degrees c +/- 3 degrees c. in secondarily infected leaves, the re ... | 1979 | 473960 |
| effects of dietary carbohydrate on cholesterol metabolism in rats and rabbits. | semipurified diets produce a marked hypercholesterolemia in rabbits and tend to elevate the level of plasma cholesterol in rats. they also decrease rates of oxidation of [26-14c]cholesterol to respiratory 14co2 and excretion of label in fecal lipid, compared with commercial feed. in both species, the hypercholesterolemia was prevented and the rate of oxidation of [26-14c]cholesterol increased by using unmodified potato starch as the carbohydrate component of the semipurified diets. potato starch ... | 1979 | 476512 |
| effect of potato inhibitor of proteolytic enzymes on activity and morphology of the rat pancreas. | the effect of the inhibitor of proteolytic enzymes present in potatoes on morphology and activity of the rat pancreas was investigated administering the inhibitor with drinking water in doses of 50 and 100 mg to two groups of rats for 20 days. on the 21st day the exocrine activity of the pancreas was evaluated by the method of duodenal perfusion after previous hormonal stimulation of pancreatic secretion (secretin and cholecystokinin-pancreozymin 1 u chr/100 g body weight), while morphology of t ... | 1979 | 495141 |
| the effect of either raw or boiled potato juice on the digestibility of a diet based on barley in pigs. | | 1979 | 504177 |
| digestibility of raw and cooked starches from legume seeds using the laboratory rat. | the in vivo digestibility and growth responses to diets containing raw and cooked legume starches were determined using male wistar rats. these starches were obtained from seven legumes including smooth- and wrinkled-seeded field peas, navy beans, kidney beans, garbanzo beans, mung beans and lentils. wheat and potato starch diets were fed for comparative purposes. with the exception of potato and the field pea starches, rats fed uncooked starch diets had higher weight gains than those fed cooked ... | 1979 | 512695 |
| comparative merits of eight popular media in aerometric studies of fungi. | although cultural surveys of airborne fungi are pursued widely, the comparative virtues of popular media have not been systematically tested. to facilitate an informed choice, eight different agar media were compared using paired single-plate exposures of identical, wind-oriented, andersen samplers. modified mehrlich's (mm), sabouraud's dextrose (sab), malt extract (malt), v8 juice (v8), and potato dextrose-rose bengal (pdar) agar were compared initially; potato dextrose (pda), malt, rose bengal ... | 1977 | 556550 |
| metabolites from microbially infected potato. part 1. structure of phytuberin. | | 1977 | 556731 |
| l-phenylalanine ammonia-lyase (maize, potato, and rhodotorula glutinis) explaining the kinetic effects of substrate modification by linear free-energy relationships. | | 1977 | 558738 |
| affinity of glucose analogs for alpha-glucan phosphorylases from rabbit muscle and potato tubers. | the action of phosphorylase b from rabbit muscle and potato phosphorylase was inhibited to various extents by several glucose analogs. like glucose itself, all of the glucosidic oxygen-substituted analogs tested in kinetic experiments showed a nonlinear competitive inhibition for muscle phosphorylase b and a linear competitive one for potato phosphorylase. 5-thio-d-glucose, one of the ring oxygen-substituted analogs, also inhibited the action of muscle phosphorylase b in the same manner, while t ... | 1977 | 560367 |
| toxicity of phytophthora infestans and alternaria solani to chick embryos. | none of the extracts and culture filtrates from growth of 9 races of phytophthora infestans on living potato tissue and potato dextrose broth are toxic to chick embryos. chloroform extracts of 4 out of 10 isolates of alternaria solani grown on potato dextrose broth showed some toxicity to chick embryos. | 1978 | 564282 |
| [human nitrogen balance under oral administration of egg proteins]. | long-term nitrogen balance periods over 17 and 24 days were performed in 5 healthy volunteers (2 females, 3 males) in order to evaluate the requirements under varying input of whole egg protein. nitrogen balances are consquently influenced in a positive or negative way by increased or decreased protein intake. the estimation of protein requirements, an important precondition for evaluation of biological value of proteins and/or amino acid mixtures, however, is not influenced as far as experiment ... | 1979 | 570550 |
| studies on thermoactinomyces sp. tm9208. i. description of the strain. | a strain of thermoactinomyces sp., tm9208, isolated from a soil sample in the taipei area showed antagonistic activity to gram positive bacteria but not to the gram negative on the potato extract agar plate by the cross streaking method. the strain showed strong starch hydrolysis, beta type of hemolysis, brownish yellow growth on the potato extract agar and green on the nutrient agar. its aerial mycelium, white to grayish white, was long and straight with short branches. the spores are single an ... | 1977 | 578130 |
| nitrogen retention of young men who consumed selection patterns of essential amino acids at a constant nitrogen intake. | six patterns of essential amino acids were devised to represent different sources or combinations of protein based on a daily intake of 6.0 g of nitrogen from these sources. in each diet, cereals furnished 70% and a specially designed amino acid mixture 30% of the total nitrogen. nitrogen retention of young men who consumed amino acids equivalent to combinations of rice and soy or egg and potato did not differ from the pattern of egg, and these patterns were superior to a combination of rice and ... | 1978 | 580544 |
| effect of non-fat dry milk on recovery of staphylococcal thermonuclease from foods. | modification of the method of tatini et al. (1976) by addition of non-fat dry milk (nfdm) to food samples and subsequent acid precipitation at ph 3.8 enhanced the recovery of staphylococcal thermonuclease (tnase) from most of 37 foods tested. the modified tnase assay method allowed detection of 10 ng (0.002 units) of the enzyme per gram of each of the following foods: ground beef, boiled egg products, whey powder, fruit-containing yogurt, dressings and spreads, potato and egg salads, and pastas, ... | 1979 | 581858 |
| magnesium absorption in the caecum of rats related to volatile fatty acids production. | rats were fed with a semi-synthetic diet with or without raw potato starch. (the level of minerals was the same in the two diets.) because of its special structure raw potato starch was not attacked by rat enzymes. the rats fed on the raw potato starch diet showed increase in caecal weight. the increase in caecal volatile fatty acids (vfa) and decrease in ph were evidence of increased microbial fermentation from the presence of substrate in the caecum. there was also an increase in the arteriove ... | 1977 | 596789 |
| [speroplast behavior in cultures of thermus ruber]. | spheroplasts spontaneously originating in cultures of thermus ruber, which are cultivated on potato-peptone media, are capable of growth and multiplication. they may reach 10 mc in diameter. spheroplasts multiply by budding which can be preceded with inner division or budding; as a result 2--9 and more protoplast bodies are formed under the "envelope" of a spheroplast. self-reproduction of spheroplasts is most active in a semiliquid potato-meat-peptone medium containing 0.2--0.3 per cent of agar ... | 1977 | 600101 |
| trichophyton fischeri sp. nov.: a saprophyte resembling trichophyton rubrum. | a new species trichophyton fischeri was isolated as a contaminant on blood agar plates. this fungus is believed to be a saprophyte. it may be confused with t. rubrum. on peptone dextrose agar plate, the growth is white and velvety to cottony. it occasionally forms furrows. the underside of the mature colony is brownish red. clavate microaleuriospores are common. trichophyton-type macroaleuriospores are produced occasionally on blood agar and potato dextrose agar. erythritol does not stimulate t. ... | 1977 | 601659 |
| naturally occurring inhibitors of intracellular proteinases. | the papain inhibitor isolated from chicken egg white inhibits the enzymatic activity of cathepsin b1 and cathepsin c. the inhibitor bears two nonoverlapping reactive sites: one binds cathepsin b1, papain, ficin, and bromelain, the other one cathepsin c. the inhibitor decreases the degree of an immunologic hypersensitive reaction, the so-called arthus reaction. a statistically significant inhibition of this immunologically developed inflammation occurs only if the inhibitor is applied intradermal ... | 1977 | 616734 |
| [veterinary herd management programme on a beef bull farm (author's transl)]. | a report on herd management advice on a beef bull farm by a multidisciplinary committee over a three-year period. eight groups, each consisting of approximately ninety-six bulls (781), were fattened and delivered. fattening of the calves was started at the age of one week and they were delivered at the age of seventy weeks. for the first ten weeks, they were individually housed in rearing pens, then they were kept in groups of eight in housings with slatted floors. studies were done to see wheth ... | 1978 | 622713 |
| nucleotide sequence and secondary structure of potato spindle tuber viroid. | the viroid of the potato spindle tuber disease (pstv) is a covalently closed ring of 359 ribonucleotides. as a result of intramolecular base pairing, a serial arrangement of double-helical sections and internal loops form a unique rod-like secondary structure. pstv is the first pathogen of a eukaryotic organism for which the complete molecular structure has been established. | 1978 | 643081 |
| the metabolism of chlorogenic acid in tuber discs of a resistant and a susceptible potato cultivar after inoculation with fusarium solani var. caeruleum [proceedings]. | | 1978 | 648730 |
| gastric cancer in rats after chronic intraperitoneal application of sap of green parts of potatoes (solanum tuberosum l.). | chronic i.p. application of the sap of green potato plants (potato-tops) induced carcinomas of the stomach in 4 out of 12 treated rats. | 1978 | 658258 |
| amino acid sequence around the pyridoxal 5'-phosphate binding site in potato phosphorylase. | the amino acid sequence around the pyridoxal 5'-phosphate binding site in potato phosphorylase was determined in order to compare it with those in phosphorylases from other sources having different regulatory properties. the potato enzyme was reduced by nabh4 in the presence of urea, carboxymethylated, and digested with chymotrypsin and trypsin. pyridoxyl peptides were isolated by the differential procedure using paper electrophoresis or deae-cellulose column chromatography. in edman degradation ... | 1978 | 659383 |
| in vivo synthesis of potato spindle tuber viroid: kinetic relationship between the circular and linear forms. | | 1978 | 664231 |
| influence of starches from different sources on protein utilization in rats. | 1. the role of the starch component of cereals and legumes on the utilization of casein-protein was studied. 2. in comparison with maize starch the legume and potato starches caused a significant lowering of net protein utilization (npu). and this reduction in npu could be partially restored by cooking the diets before they were fed to the rats. 3. it is suggested that in npu studies involving rats the experimental diet should always be cooked so that the conclusions drawn may be applicable to h ... | 1978 | 666992 |
| an investigation of the possible immunological relationship between allergen extracts from birch pollen, hazelnut, potato and apple. | in a retrospective study on selected group of patients, the coincidence of birch pollen allergy and a clinically relevant positive prick test reaction to apples and potatoes was confirmed. immunochemical comparison using the crossed line immunoelectrophoresis technique (clie) confirmed partial identity between birch pollen and hazelnut. by the same method no partial immunological identity between birch pollen and extracts and fresh peel from apples and potatoes was found. however, both apples an ... | 1978 | 668340 |
| analysis of terminal structures of rna from potato virus x. | the 5'-end structure of potato virus x rna was determined following enzymatic methylation in vitro. a single 3h-methyl group was introduced into the 2'-position of the 5'-penultimate residue and the end structure was determined as m7gpppg(m)pap(xp)3g. this part of the rna apparently is involved in binding to ribosomes since it can be partially protected against rnase digestion by wheat germ 40s ribosomes. pvx rna was not retained by poly(u)-sepharose, indicating that it does not contain a 3'-ter ... | 1978 | 673859 |
| [on the effect of some morpholinium compounds on the multiplication of rna viruses (author's transl)]. | in the present paper the authors report about the effect of n,n-dimethylmorpholinium chloride (dmmc), n,n-dimethyl-2-oxomorpholinium chloride (dmomc), and n,n-diethyl-2-oxomorpholinium chloride (deomc) on the multiplication of tobacco mosaic virus (tmv), potato virus x (pvx), and cucumber mosaic virus (cmv) as well as the plaque formation by the rna phages m 12, f 2, and qbeta. the used morpholinium compounds do not influence strikingly the multiplication of phytoviruses. the results found by se ... | 1978 | 685536 |
| teratogenic potential of blighted potato concentrate in rabbits, hamsters, and miniature swine. | freeze-dried preparations of potatoes infected with phytophthora infestans or alternaria solani were fed to pregnant hamsters, rabbits, and miniature swine. no evidence was observed that these diets or the extracts of respective blights were capable of producing teratologic effects in hamsters. lesions of neurulation and anencephaly were noticed in rabbits and swine, respectively. the need for further pharmacological and toxicological evaluation in sensitive species is emphasized. | 1978 | 694778 |
| race identification of pseudomonas solanacearum e. f. smith in egypt. | twenty one isolates of pseudomonas solanacearum e. f. smith, which does not occur on hosts other than potato in egypt, obtained from diseased potato tubers, were collected from different localities in egypt and used in this study. these 21 isolates were found pathogenic to potato plants, but they were not pathogenic to tobacco and banana plants. all the 21 isolates caused only a yellowish discoloration of the infiltrated area of tobacco leaves by 48 hrs after inoculation and produced low melanin ... | 1978 | 696042 |
| tests in vitro and in pots with certain chemicals for inhibition of pseudomonas solanacearum. | twenty one isolates of pseudomonas solanacearum e. f. smith (race 3) from various parts of egypt were inhibited in vitro by ampicillin, chloramphenicol, kanamycin, oxytetracycline. tetracycline, penicillin g, streptomycin, nabam (dithane a-40), maneb (dithane m-22), zinc-ion maneb complex (dithane m-45), and the insecticide chlorthion. on the basis of in vitro-sensitivity to the selected 11 chemicals the 21 isolates could not be separated into different groups. carbendazin (bavistin), benomyl, d ... | 1978 | 696045 |
| on rapeseed meals. part xxvi. some remarks on the biological value of rapeseed meal proteins after silage. | the influence of propionic bacteria on the biological value of potato-rapeseed meal protein ensilage was investigated. the inoculation of the ensilage with propionibacterium petersoni t 112 led to the reduction of the content of goitrogenous compounds (isothiocyanates and oxazolidinethiones) and to an increase of the nutritive value (npu, per) of the rapeseed protein. the increase of the protein value is greater by the application of propionic bacteria than by toasting of rapeseed meal. | 1978 | 703817 |
| dietary fiber and cholesterol metabolism in rabbits and rats. | rabbits fed semipurified diets have elevated plasma cholesterol levels, and they oxidize [26-14c]cholesterol to expired 14co2 more slowly than rabbits on a natural ingredient diet. addition of several different types of fibrous material to a semipurified diet failed to prevent the hypercholesterolemic response. rats on semipurified diets also oxidized [26-14c]cholesterol more slowly and tended to have somewhat higher plasma cholesterol levels than rats on commercial feed. cholesterol oxidation w ... | 1978 | 707374 |
| food sensitivity reported by patients with asthma and hay fever. a relationship between food sensitivity and birch pollen-allergy and between food sensitivity and acetylsalicylic acid intolerance. | among adult patients with bronchial asthma and/or allergic rhinitis undergoing allergological investigation with skin test, nasal provocation test and rast, 1129 answered a questionaire regarding food sensitivity (fs). 276 (24%) of the patients reported some kind of allergic symptoms on eating or handling various foods, of which hazel nut, apple and shell fish were the most often named. females reported fs more often than males. a correlation was found between birch pollen allergy and fs with nu ... | 1978 | 717703 |
| cyclopamine and related steroidal alkaloid teratogens: their occurrence, structural relationship, and biologic effects. | a spontaneous congenital deformity is produced in lambs whose dams consume veratrum californicum on the 14th day of gestation. the deformity is generally expressed as cyclopia, cebocephaly, anophthalmia, or microphthalmia. this teratogenic effect is produced by certain steroidal alkaloid teratogens from the plant - most notably the compound cyclopamine. cyclopamine is a c-nor-d-homo steroid with fused furanopiperidine rings e and f at right angles to the plane of the steroid because of spiro att ... | 1978 | 723484 |
| potato spindle tuber viroid: circular dichroism spectrum and physical chemical studies of its interaction with ethidium bromide. | the presence and extent of base-paired secondary structure in the protein-free potato spindle tuber viroid rna has been investigated by spectroscopic techniques. dye-binding studies with the intercalating dye, ethidium bromide, indicate the presence of base-paired regions in the molecule. variation of the induced chirality in the dye with the dye to rna phosphate ratio indicate the presence of two base-paired regions in the molecule. | 1978 | 728834 |
| 5'-terminal 7-methylguanosine and mrna function. the effect of enzymatic decapping and of cap analogs on translation of tobacco-mosaic-virus rna and globin mrna in vitro. | 1. decapped tobacco mosaic virus (tmv) rna and rabbit globin mrna were prepared by enzymic treatment of rnas with nucleotide pyrophosphatase purified from potato. the extent of removal of 5'-terminal 7-methylguanosine 5'-monophosphate (m7gmp) from tmv rna was at least 97% as estimated by labeling of the 5' termini in vitro with s-adenosyl[methyl-3h]methionine catalysed by vaccinia virus methyltransferases. 2. the effect of enzymic decapping was compared with the effect of cap analogs on mrnas tr ... | 1978 | 729595 |
| [structurally dependent effects of substituted thioureas on the concentration of potato virus x in nicotiana tabacum l. "samsun" (author's transl)]. | a study has been made to investigate the effects of n-alkyl- and aryl-substituted thioureas as well as n,n'-alkyl-alkyl-, alkyl-aryl-, and aryl-aryl-substituted thioureas on the multiplication of pvx in nicotiana tabacum l. "samsun". the virus concentrations of the treated plants were serologically determined and compared with these of untreated controls. the weak antiphytoviral effect of nonsubstituted thiourea remained virtually unchanged in n,n'-dimethyl thiourea, and in n,n,n'-trimethyl thio ... | 1978 | 749413 |
| understanding the relationship between lateral spread of freeze and depth of freeze. | the predictable relationship between lateral spread of freeze and depth of freeze is a keystone concept in the clinical evaluation of "depth dose" of cryosurgical procedures. this relationship can be readily determined and understood by carrying out simple experiments using potato models and checked directly by measurements on patients. when using specialized treatment methods such as the "cone-spray" technique with liquid nitrogen, it is necessary to limit the technique to properly selected cas ... | 1979 | 759460 |
| low-intensity microwave radiation and the virulence of agrobacterium tumefaciens strain b6. | when virulent cells of agrobacterium tumefaciens strain b6 were exposed to low-level microwave radiation at a frequency of 10,000 mhz and an intensity of 0.58 mw/cm2 for 30 to 120 min, a 30 to 60% decrease in their ability to produce tumors on potato and turnip disks was observed. this microwave exposure did not affect the viability of these bacteria or their ability to attach to a tumor-binding site nor did it induce thermal shock. this loss of virulence was reversible within 12 h. | 1979 | 760631 |
| definition of dietary fiber and hypotheses that it is a protective factor in certain diseases. | crude fiber (cf) is the residue of plant food left after extraction by dilute acid followed by dilute alkali. dietary fiber (df), a new term, is the residue of plant food resistant to hydrolysis by human alimentary enzymes. df is composed of cellulose, hemicellulose, and lignin; these constituents are not reported in food tables. for instance, whole wheatmeal has df about 11%, cf about 2%. it is suggested that a new term, dietary fiber complex (dfc), should include all substances of df plus all ... | 1976 | 773166 |
| epidemic streptococcal sore throat following a community picnic. | following a picnic, nearly half of the persons who attended became ill. sixty-three of 139 throat cultures were positive for group a beta-hemolytic streptococci. forty-seven of the positive cultures were available for typing, 34 of which harbored the epidemic strain, group a beta-hemolytic streptococcus type t-3/13/b3264. this contrasted with two of 204 controls who had not attended, but harbored the epidemic strain. food preferences and illness rates implicated the potato salad, and from it the ... | 1976 | 787568 |
| regeneration of plants from protoplasts of potato virus x-infected tobacco leaves. | | 1975 | 808032 |
| ultrastructure of acid- and enzyme-modified cross-linked potato starch. | | 1975 | 809070 |
| proteinase inhibitor ii from potatoes: isolation and characterization of its protomer components. | proteinase inhibitor ii, an inhibitor of chymotrypsin and trypsin, is a heat-stable protein with a dimeric molecular weight of 21 000 that is a component of russet burbank potato tubers. four monomeric isoinhibitor species of molecular weight 10 500 comprise inhibitor ii and were isolated by chromatography on phosphocellulose in 8 m urea. upon removal of the urea, each monomeric species dimerized to yield homogeneous dimers. the three major protomer species, called b, c, and d, and their homogen ... | 1976 | 821519 |
| effect on the reproductive functions of female rhesus monkeys of feeding irradiated wheat flour and potato diet. | we investigated the effects on reproductive functions of feeding adult rhesus monkeys on a diet of irradiated wheat flour and potato. wheat flour and potatoes were exposed to 75000 and 10000 rad, respectively, of gamma-irradiation and then fed to the animals. various parameters of reproductive function were compared with those of animals fed on unirradiated food. these were: external changes in the reproductive organs, menstrual cycle, vaginal cytology, assay of urinary hormones (oestrogens and ... | 1976 | 823122 |
| protein quality of precipitate from waste effluent of potato chip processing measured by biological methods. | | 1976 | 823188 |
| reconstitution of potato virus x in vitro. iii. evidence for a role for hydrophobic interactions. | | 1977 | 835246 |
| size and secondary structure of potato spindle tuber viroid. | | 1977 | 841846 |
| regeneration of plants from prtoplasts of potato virus x-infected tobacco leaves. ii. influence of virazole on the frequency of infection. | | 1977 | 860402 |
| partial purification and characterization of the potato virus y helper component. | | 1977 | 860406 |