| isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mrna levels during fruit ripening. | to determine the relationship between invertase gene expression and glucose and fructose accumulation in ripening tomato fruit, fruit vacuolar invertase cdna and genomic clones from the cultivated species, lycopersicon esculentum cv. uc82b, and a wild species, lycopersicon pimpinellifolium, were isolated and characterized. the coding sequences of all cdna clones examined are identical. by comparison to the known amino acid sequence of mature l. esculentum fruit vacuolar invertase, a putative sig ... | 1993 | 8095164 |
| genetic mapping of a wide spectrum nematode resistance gene (hero) against globodera rostochiensis in tomato. | the hero gene confers resistance to a wide spectrum of pathotypes of the potato cyst nematode globodera rostochiensis. this gene has been introgressed from the wild tomato species lycopersicon pimpinellifolium into the cultivated tomato. we have used rflp and rapd analysis for the targeted search of the l. pimpinellifolium into the cultivated tomato. we have used rflp and rapd analysis for the targeted search of the l. pimpinellifolium segment. the resistant line la 1792 contains a single introg ... | 1995 | 8664498 |
| alleles of pto and fen occur in bacterial speck-susceptible and fenthion-insensitive tomato cultivars and encode active protein kinases. | the pto gene was derived originally from the wild tomato species lycopersicon pimpinellifolium and confers resistance to pseudomonas syringae pv tomato strains expressing the avirulence gene avrpto. the fen gene is also derived from l. pimpinellifolium and confers sensitivity to the insecticide fenthion. we have now isolated and characterized the alleles of pto and fen from cultivated tomato, l. esculentum, and designated them pto and fen. high conservation of genome organization between the two ... | 1997 | 9014365 |
| specific hr-associated recognition of secreted proteins from cladosporium fulvum occurs in both host and non-host plants. | the resistance of tomato (lycopersicon esculentum) to the pathogenic fungus cladosporium fulvum complies with the gene-for-gene concept. host resistance is based on specific recognition of extracellular fungal proteins, resulting in a hypersensitive response (hr). five proteins secreted by c. fulvum were purified and the encoding cdna clone was obtained from two novel ones among them. various tomato breeding lines and accessions of lycopersicon pimpinellifolium were tested for their recognitiona ... | 2000 | 10998185 |
| ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene pto. | we have investigated the origin of the pto disease resistance (r) gene that was previously identified in the wild tomato species lycopersicon pimpinellifolium and isolated by map-based cloning. pto encodes a serine-threonine protein kinase that specifically recognizes strains of pseudomonas syringae pv. tomato (pst) that express the avirulence gene avrpto. we examined an accession of the distantly related wild species lycopersicon hirsutum var. glabratum that exhibits avrpto-specific resistance ... | 2001 | 11172075 |
| potentiation by a novel alkaloid glycoside adjuvant of a protective cytotoxic t cell immune response specific for a preerythrocytic malaria vaccine candidate antigen. | we have recently demonstrated that the novel glycoalkaloid tomatine, derived from leaves of the wild tomato lycopersicon pimpinellifolium, can act as a powerful adjuvant for the elicitation of antigen-specific cd8+ t cell responses. here, we have extended our previous investigation with the model antigen ovalbumin to an established malaria infection system in mice and evaluated the cellular immune response to a major preerythrocytic stage malaria vaccine candidate antigen when administered with ... | 2001 | 11457540 |
| arabinogalactan proteins, pollen tube growth, and the reversible effects of yariv phenylglycoside. | arabinogalactan proteins (agps) are abundant complex macromolecules involved in both reproductive and vegetative plant growth. they are secreted at pollen tube tips in lilium longiflorum. here, we report the effect of the (beta-d-glucosyl)3 yariv phenylglycoside, known to interact with agps, on pollen tube extension in several plant species. in annona cherimola the yariv reagent clearly inhibited pollen tube extension within 1-2 h of treatment, as demonstrated previously for l. longiflorum, but ... | 2002 | 11926071 |
| agrosuppression: a bioassay for the hypersensitive response suited to high-throughput screening. | we describe a novel method, agrosuppression, that addresses the need for an assay of the hypersensitive response (hr) in intact plants that is rapid and adapted to high-throughput functional screening of plant and pathogen genes. the agrosuppression assay is based on inoculation of intact plants with a mixture of agrobacterium tumefaciens strains carrying (i) a binary plasmid with one or more candidate hr-inducing genes and (ii) a tumor-inducing (oncogenic) t-dna. in the absence of hr induction, ... | 2003 | 12580277 |
| population structure and phylogeography of solanum pimpinellifolium inferred from a nuclear gene. | phylogeographical studies are emerging as a powerful tool for understanding the population structure and evolution of wild relatives of crop species. because of their value as genetic resources, there is great interest in exploring the distribution of variation in wild relatives of cultivated plants. in this study, we use sequence variation from the nuclear gene, fruit vacuolar invertase (vac), to investigate the population history of solanum pimpinellifolium. solanum pimpinellifolium is a close ... | 2004 | 15189210 |
| pervasive purifying selection characterizes the evolution of i2 homologs. | we sampled 384 sequences related to the solanum pimpinellifolium (=lycopersicon pimpinellifolium) disease resistance (r) gene 12 from six species, potato, s. demissum, tomato, eggplant, pepper, and tobacco. these species represent increasing phylogenetic distance from potato to tobacco, within the family solanaceae. using sequence data from the nucleotide binding site (nbs) region of this gene, we tested models of gene family evolution and inferred patterns of selection acting on the nbs gene re ... | 2006 | 16570659 |
| the solanum pimpinellifolium cf-ecp1 and cf-ecp4 genes for resistance to cladosporium fulvum are located at the milky way locus on the short arm of chromosome 1. | the interaction between tomato and the leaf mould pathogen cladosporium fulvum is an excellent model to study gene-for-gene interactions and plant disease resistance gene evolution. most cf genes were introgressed into cultivated tomato (solanum lycopersicum) from wild relatives such as s. pimpinellifolium and novel cf-ecp genes were recently identified in this species. our objective is to isolate cf-ecp1, cf-ecp2, cf-ecp4 and cf-ecp5 to increase our understanding of cf gene evolution, and the m ... | 2007 | 17874062 |
| micro-electrode flux estimation confirms that the solanum pimpinellifolium cu3 mutant still responds to systemin. | in this study, we introduce the micro-electrode ion flux estimation technique as a sensitive and accurate technique to study systemin-induced changes in ion fluxes from isolated nearly intact plant tissues. our results demonstrate the effectiveness and value of the micro-electrode ion flux estimation technique to monitor and characterize those elicitor-induced ion flux changes from intact tissues. we used the method to monitor the systemin-induced changes in ion fluxes from leaf tissue of variou ... | 2008 | 18055585 |
| characterization of hypersensitive resistance to bacterial spot race t3 (xanthomonas perforans) from tomato accession pi 128216. | bacterial spot of tomato is caused by four species of xanthomonas. the accession pi 128216 (solanum pimpinellifolium) displays a hypersensitive reaction (hr) to race t3 strains (predominantely xanthomonas perforans). we developed an inbred backcross (ibc) population (bc(2)s(5), 178 families) derived from pi 128216 and oh88119 (s. lycopersicum) as the susceptible recurrent parent for simultaneous introgression and genetic analysis of the hr response. these ibc families were evaluated in the green ... | 2009 | 19671005 |
| colonization of tomato plants by salmonella enterica is cultivar dependent, and type 1 trichomes are preferred colonization sites. | nontyphoid salmonellosis caused by salmonella enterica is the most common bacterial food-borne illness in humans, and fresh produce, including tomatoes, is a common vehicle. accumulating data indicate that human enteric pathogenic bacteria, including s. enterica, interact actively with plants. tomato plants were inoculated with s. enterica to evaluate plausible contamination routes and to determine if the tomato cultivar affects s. enterica colonization. s. enterica population levels on tomato l ... | 2010 | 21075871 |
| whitefly resistance traits derived from the wild tomato solanum pimpinellifollium affect the preference and feeding behavior of bemisia tabaci and reduce the spread of tomato yellow leaf curl virus. | breeding of tomato genotypes that limit whitefly (bemisia tabaci) access and feeding might reduce the spread of tomato yellow leaf curl virus (tylcv), a begomovirus (genus begomovirus, family geminiviridae) that is the causal agent of tomato yellow leaf curl disease. tylcv is restricted to the phloem and is transmitted in a persistent manner by b. tabaci. the tomato breeding line abl 14-8 was developed by introgressing type iv leaf glandular trichomes and secretion of acylsucroses from the wild ... | 2011 | 21615206 |
| a solanum lycopersicum x solanum pimpinellifolium linkage map of tomato displaying genomic locations of r-genes, rgas, and candidate resistance/defense-response ests. | we have identified an accession (la2093) within the tomato wild species solanum pimpinellifolium with many desirable characteristics, including biotic and abiotic stress tolerance and good fruit quality. to utilize the full genetic potential of la2093 in tomato breeding, we have developed a linkage map based on an f(2) population of a cross between la2093 and a tomato breeding line, using 115 rflp, 94 est, and 41 rga markers. the map spanned 1002.4 cm of the 12 tomato chromosomes with an average ... | 2008 | 19223983 |
| rearrangements in the cf-9 disease resistance gene cluster of wild tomato have resulted in three genes that mediate avr9 responsiveness. | cf resistance genes in tomato confer resistance to the fungal leaf pathogen cladosporium fulvum. both the well-characterized resistance gene cf-9 and the related 9dc gene confer resistance to strains of c. fulvum that secrete the avr9 protein and originate from the wild tomato species lycopersicon pimpinellifolium. we show that 9dc and cf-9 are allelic, and we have isolated and sequenced the complete 9dc cluster of l. pimpinellifolium la1301. this 9dc cluster harbors five full-length cf homologs ... | 2004 | 15579714 |
| structure-function analysis of cf-9, a receptor-like protein with extracytoplasmic leucine-rich repeats. | the tomato (lycopersicon pimpinellifolium) resistance protein cf-9 belongs to a large class of plant proteins with extracytoplasmic leu-rich repeats (elrrs). elrr proteins play key roles in plant defense and development, mainly as receptor-like proteins or receptor-like kinases, conferring recognition of various pathogen molecules and plant hormones. we report here a large-scale structure-function analysis of an elrr protein. a total of 66 site-directed mutants of cf-9 were analyzed for activity ... | 2005 | 15722474 |
| comprehensive tissue-specific transcriptome analysis reveals distinct regulatory programs during early tomato fruit development. | fruit formation and early development involve a range of physiological and morphological transformations of the various constituent tissues of the ovary. these developmental changes vary considerably according to tissue type, but molecular analyses at an organ-wide level inevitably obscure many tissue-specific phenomena. we used laser-capture microdissection coupled to high-throughput rna sequencing to analyze the transcriptome of ovaries and fruit tissues of the wild tomato species solanum pimp ... | 2015 | 26099271 |
| [studies on the role of silicic acid in the development of higher plants]. | germanium acid, a specific inhibitor of the silicic acid metabolism in diatoms, inhibits the growth of sinapis alba, lemna minor, wolffia arrhiza, nicotiana tabacum, tradescantia spec, zinnia elegans, and secale cereale when applied in the same concentrations as those used in the case of diatoms (15-75 μg geo2/ml medium). the growth of aspergillus niger, phycomyces blakesleanus, escherichia coli k 12, euglena gracilis and pandorina morum is not influenced by these and higher concentrations of ge ... | 1967 | 24549376 |
| genome-wide identification and expression profiling of tomato hsp20 gene family in response to biotic and abiotic stresses. | the hsp20 genes are involved in the response of plants to environment stresses including heat shock and also play a vital role in plant growth and development. they represent the most abundant small heat shock proteins (shsps) in plants, but little is known about this family in tomato (solanum lycopersicum), an important vegetable crop in the world. here, we characterized heat shock protein 20 (slhsp20) gene family in tomato through integration of gene structure, chromosome location, phylogeneti ... | 2016 | 27582749 |
| the tomato i gene for fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on sobir1 and serk3/bak1. | we have identified the tomato i gene for resistance to the fusarium wilt fungus fusarium oxysporum f. sp. lycopersici (fol) and show that it encodes a membrane-anchored leucine-rich repeat receptor-like protein (lrr-rlp). unlike most other lrr-rlp genes involved in plant defence, the i gene is not a member of a gene cluster and contains introns in its coding sequence. the i gene encodes a loopout domain larger than those in most other lrr-rlps, with a distinct composition rich in serine and thre ... | 2017 | 27995670 |
| development of a high-resolution melting marker for selecting fusarium crown and root rot resistance in tomato. | fusarium crown and root rot is a severe fungal disease of tomato caused by fusarium oxysporum f. sp. radicis-lycopersici (forl). in this study, the genomic location of the forl-resistance locus was determined using a set of molecular markers on chromosome 9 and an f2 population derived from forl-resistant inbred 'av107-4' (solanum lycopersicum) × susceptible 'l3708' (solanum pimpinellifolium). bioassay performed using korean forl strain kacc 40031 showed single dominant inheritance of forl resis ... | 2016 | 26853773 |
| dual disease resistance mediated by the immune receptor cf-2 in tomato requires a common virulence target of a fungus and a nematode. | plants lack the seemingly unlimited receptor diversity of a somatic adaptive immune system as found in vertebrates and rely on only a relatively small set of innate immune receptors to resist a myriad of pathogens. here, we show that disease-resistant tomato plants use an efficient mechanism to leverage the limited nonself recognition capacity of their innate immune system. we found that the extracellular plant immune receptor protein cf-2 of the red currant tomato (solanum pimpinellifolium) has ... | 2012 | 22675118 |
| a new strain of tomato severe leaf curl virus and a unique variant of tomato yellow leaf curl virus from mexico. | the complete genome sequence of a distinct variant of tomato yellow leaf curl virus-israel (tylcv-il) and the dna-a sequence of a new strain of tomato severe leaf curl virus (toslcv) isolated in san luis potosi, mexico, are described and analyzed. the tylcv-il[mx:slp:11] variant differs from all tylcv-il isolates described so far by a unique 42-nt duplicated sequence comprising a part of the conserved stem-loop element of the virion-strand replication origin and adjacent regulatory sequences. ty ... | 2012 | 22684489 |
| a new eif4e1 allele characterized by rnaseq data mining is associated with resistance to potato virus y in tomato albeit with a low durability. | allele mining on susceptibility factors offers opportunities to find new sources of resistance among crop wild relatives for breeding purposes. as a proof of concept, we used available rnaseq data to investigate polymorphisms among the four tomato genes encoding translation initiation factors [eif4e1 and eif4e2, eifiso4e and the related gene new cap-binding protein(ncbp)] to look for new potential resistance alleles to potyviruses. by analysing polymorphism among rnaseq data obtained for 20 toma ... | 2016 | 27655175 |
| natural variation in tomato reveals differences in the recognition of avrpto and avrptob effectors from pseudomonas syringae. | the pto protein kinase from solanum pimpinellifolium interacts with pseudomonas syringae effectors avrpto or avrptob to activate effector-triggered immunity. the previously solved crystal structures of the avrpto-pto and avrptob-pto complexes revealed that pto binds each effector through both a shared and a unique interface. here we use natural variation in wild species of tomato to further investigate pto recognition of these two effectors. one species, solanum chmielewskii, was found to have m ... | 2016 | 26993968 |
| genome-wide analysis of phylogeny, expression profile and sub-cellular localization of skp1-like genes in wild tomato. | skp1 is a core component of scf complex, a major type of e3 ubiquitin ligase catalyzing the last step in ubiquitin-mediated protein degradation pathway. in present study, skp1 gene family in solanum pimpinellifolium (ssk), a wild species of tomato, was investigated. a total of 19 ssk genes were identified through homologous search. their chromosomal locations, gene structures, phylogeny, expression profiles, sub-cellular localizations and protein-protein interaction patterns with putative f-box ... | 2015 | 26259179 |
| a novel method for the evaluation of virus-induced gene silencing efficiency. | virus-induced gene silencing (vigs) is an important tool for studying gene function. however, a number of factors highly restrict the application of vigs, such as unstable efficiency and tissue-specific silencing. we developed a novel evaluation method for improving the applicability of vigs vectors. in this method, 4 indexes were defined and utilized to evaluate vigs efficiency by silencing the endogenous phytoene desaturase (pds) gene with a tobacco rattle virus-based vigs vector. to illustrat ... | 2014 | 25501154 |
| a jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence. | whiteflies damage tomatoes mostly via the viruses they transmit. cultivated tomatoes lack many of the resistances of their wild relatives. in order to increase protection to its major pest, the whitefly bemisia tabaci and its transmitted tomato yellow leaf curl virus (tylcv), we introgressed a trichome-based resistance trait from the wild tomato solanum pimpinellifolium into cultivated tomato, solanum lycopersicum. the tomato backcross line bc5s2 contains acylsucrose-producing type-iv trichomes, ... | 2016 | 27920785 |
| reassessment of qtls for late blight resistance in the tomato accession l3708 using a restriction site associated dna (rad) linkage map and highly aggressive isolates of phytophthora infestans. | tomato late blight caused by the oomycete pathogen phytophthora infestans (mont.) de bary is a major threat to tomato production in cool and wet environments. intensified outbreaks of late blight have been observed globally from the 1980s, and are associated with migration of new and more aggressive populations of p. infestans in the field. the objective of this study was to reassess late blight resistance in the wild tomato accession l3708 (solanum pimpinellifolium l.) against pathogens of diff ... | 2014 | 24788810 |
| the ph-3 gene from solanum pimpinellifolium encodes cc-nbs-lrr protein conferring resistance to phytophthora infestans. | ph-3 is the first cloned tomato gene for resistance to late blight and encodes a cc-nbs-lrr protein. late blight, caused by phytophthora infestans, is one of the most destructive diseases in tomato. the resistance (r) gene ph-3, derived from solanum pimpinellifolium l3708, provides resistance to multiple p. infestans isolates and has been widely used in tomato breeding programmes. in our previous study, ph-3 was mapped into a region harbouring r gene analogues (rga) at the distal part of long ar ... | 2014 | 24756242 |
| fine mapping of the ph-3 gene conferring resistance to late blight (phytophthora infestans) in tomato. | late blight, caused by the oomycete pathogen phytophthora infestans (mont.) de bary, is a devastating disease for tomato and potato crops. in the past decades, many late blight resistance (r) genes have been characterized in potato. in contrast, less work has been conducted on tomato. the ph-3 gene from solanum pimpinellifolium was introgressed into cultivated tomatoes and conferred broad-spectrum resistance to p. infestans. it was previously assigned to the long arm of chromosome 9. in this stu ... | 2013 | 23921955 |
| the ring finger e3 ligase spring is a positive regulator of salt stress signaling in salt-tolerant wild tomato species. | protein ubiquitination in plants plays critical roles in many biological processes, including adaptation to abiotic stresses. previously, ring finger e3 ligase has been characterized during salt stress response in several plant species, but little is known about its function in tomato. here, we report that spring, a stress-inducible gene, is involved in salt stress signaling in wild tomato species solanum pimpinellifolium 'pi365967'. in vitro ubiquitination assay revealed that spring is an e3 ub ... | 2016 | 26786853 |
| trehalase: a new pollen enzyme. | pollen from 5 plant species (lycopersicon pimpinellifolium mill., hermerocallis minor mill., galtonia condicans decne., camellia japonica l., and lathyrus odoratus l.) representing 4 families germinated well in media containing trehalose as the sole carbon source. data are presented indicating that pollen metabolized this disaccharide for germination and subsequent pollen-tube growth; the sugar was not merely an osmoregulator. an inhibitor of trehalase activity depressed germination in trehalose ... | 1969 | 5379538 |
| identification, introgression, and validation of fruit volatile qtls from a red-fruited wild tomato species. | volatile organic compounds (vocs) are major determinants of fruit flavor, a primary objective in tomato breeding. a recombinant inbred line (ril) population consisting of 169 lines derived from a cross between solanum lycopersicum and a red-fruited wild tomato species solanum pimpinellifolium accession (sp) was characterized for vocs in three different seasons. correlation and hierarchical cluster analyses were performed on the 52 vocs identified, providing a tool for the putative assignation of ... | 2016 | 28040800 |
| qtl analysis of horticultural traits differentiating the cultivated tomato from the closely related species lycopersicon pimpinellifolium. | molecular markers were used to map and characterize quantitative trait loci (qtls) for several characters of agronomic and biological importance in an interspecific backcross of tomato. the parents of the cross were an elite processing inbred lycopersicon esculentum cv 'm82-1-7' and the closely related red-fruited wild species l. pimpinellifolium (la1589). a total of 257 bc1 plants were grown under field conditions in ithaca, new york and scored for 19 quantitative traits. a genetic linkage map ... | 1996 | 24166620 |
| targeted recombination between homologous chromosomes for precise breeding in tomato. | homologous recombination (hr) between parental chromosomes occurs stochastically. here, we report on targeted recombination between homologous chromosomes upon somatic induction of dna double-strand breaks (dsbs) via crispr-cas9. we demonstrate this via a visual and molecular assay whereby dsb induction between two alleles carrying different mutations in the phytoene synthase (psy1) gene results in yellow fruits with wild type red sectors forming via hr-mediated dsb repair. we also show that in ... | 0 | 28548094 |
| exploring new alleles involved in tomato fruit quality in an introgression line library of solanum pimpinellifolium. | we have studied a genomic library of introgression lines from the solanum pimpinellifolium accession to-937 into the genetic background of the "moneymaker" cultivar in order to evaluate the accession's breeding potential. overall, no deleterious phenotypes were observed, and the plants and fruits were phenotypically very similar to those of "moneymaker," which confirms the feasibility of translating the current results into elite breeding programs. we identified chromosomal regions associated wi ... | 0 | 27582742 |
| heterogeneous evolutionary processes affect r gene diversity in natural populations of solanum pimpinellifolium. | resistance (r) genes of plants are responsible for pathogen recognition and encode proteins that trigger a cascade of responses when a pathogen invades a plant. r genes are assumed to be under strong selection, but there is limited knowledge of the processes affecting r gene diversity in the wild. in this study, dna sequence variation of cf-2 homologs was surveyed in populations of solanum pimpinellifolium, a wild relative of the cultivated tomato. the cf-2 locus is involved in resistance to str ... | 2004 | 15583124 |
| developmental control of promoter activity is not responsible for mature onset of cf-9b-mediated resistance to leaf mold in tomato. | cf-9 confers resistance to tomato seedlings and mature plants against cladosporium fulvum races expressing the avr9 elicitor. it is the central member of a cluster of five paralogous genes in an introgressed segment of chromosome 1 derived from lycopersicon pimpinellifolium. the other four genes have been named hcr9-9a, hcr9-9b, hcr9-9d, and hcr9-9e. hcr9-9b, here designated cf-9b, encodes weaker resistance than cf-9, recognizes a different elicitor, and protects only mature plants from infectio ... | 2002 | 12423015 |
| a tomato cysteine protease required for cf-2-dependent disease resistance and suppression of autonecrosis. | little is known of how plant disease resistance (r) proteins recognize pathogens and activate plant defenses. rcr3 is specifically required for the function of cf-2, a lycopersicon pimpinellifolium gene bred into cultivated tomato (lycopersicon esculentum) for resistance to cladosporium fulvum. rcr3 encodes a secreted papain-like cysteine endoprotease. genetic analysis shows rcr3 is allelic to the l. pimpinellifolium ne gene, which suppresses the cf-2-dependent autonecrosis conditioned by its l. ... | 2002 | 11976458 |
| intragenic recombination generated two distinct cf genes that mediate avr9 recognition in the natural population of lycopersicon pimpinellifolium. | resistance gene cf-9 of cultivated tomato (lycopersicon esculentum) confers recognition of the avr9 elicitor protein of the fungal pathogen cladosporium fulvum. the cf-9 locus, containing cf-9 and four homologs (hcr9s), originates from lycopersicon pimpinellifolium (lp). we examined naturally occurring polymorphism in hcr9s that confer avr9 recognition in the lp population. avr9 recognition occurs frequently throughout this population. in addition to cf-9, we discovered a second gene in lp, desi ... | 2001 | 11517316 |
| overexpression of spwrky1 promotes resistance to phytophthora nicotianae and tolerance to salt and drought stress in transgenic tobacco. | wrky transcription factors are key regulatory components of plant responses to biotic and abiotic stresses. spwrky1, a pathogen-induced wrky gene, was isolated from tomato (solanum pimpinellifolium l3708) using in silico cloning and reverse transcriptase-polymerase chain reaction (rt-pcr) methods. spwrky1 expression was significantly induced following oomycete pathogen infection and treatment with salt, drought, salicylic acid (sa), methyl jasmonate (meja) and abscisic acid (aba). overexpression ... | 2015 | 25496091 |
| the corky root rot pathogen pyrenochaeta lycopersici secretes a proteinaceous inducer of cell death affecting host plants differentially. | pathogenic isolates of pyrenochaeta lycopersici, the causal agent of corky root rot of tomato, secrete cell death in tomato 1 (cdit1), a homodimeric protein of 35 kda inducing cell death after infiltration into the leaf apoplast of tomato. cdit1 was purified by fast protein liquid chromatography, characterized by mass spectrometry and cdna cloning. its activity was confirmed after infiltration of an affinity-purified recombinant fusion of the protein with a c-terminal polyhistidine tag. cdit1 is ... | 2012 | 22708726 |
| the making of a bell pepper-shaped tomato fruit: identification of loci controlling fruit morphology in yellow stuffer tomato. | the heirloom tomato cultivar yellow stuffer produces fruit that are similar in shape and structure to fruit produced by the bell pepper varieties of garden pepper. to determine the genetic basis of this extreme fruit type in tomato, quantitative trait loci (qtl) analysis was performed on an f(2) population derived from a cross between yellow stuffer and the related species, lycopersicon pimpinellifolium, which produces a small, round fruit typical of most wild species. f(2) plants were analyzed ... | 2003 | 12835939 |
| evidence of cryptic introgression in tomato (solanum lycopersicum l.) based on wild tomato species alleles. | many highly beneficial traits (e.g. disease or abiotic stress resistance) have been transferred into crops through crosses with their wild relatives. the 13 recognized species of tomato (solanum section lycopersicon) are closely related to each other and wild species genes have been extensively used for improvement of the crop, solanum lycopersicum l. in addition, the lack of geographical barriers has permitted natural hybridization between s. lycopersicum and its closest wild relative solanum p ... | 2012 | 22871151 |
| comparative functional genomic analysis of solanum glandular trichome types. | glandular trichomes play important roles in protecting plants from biotic attack by producing defensive compounds. we investigated the metabolic profiles and transcriptomes to characterize the differences between different glandular trichome types in several domesticated and wild solanum species: solanum lycopersicum (glandular trichome types 1, 6, and 7), solanum habrochaites (types 1, 4, and 6), solanum pennellii (types 4 and 6), solanum arcanum (type 6), and solanum pimpinellifolium (type 6). ... | 2011 | 21098679 |
| multilocus sequence data reveal extensive departures from equilibrium in domesticated tomato (solanum lycopersicum l.). | limited genetic variation has been observed within tomato (solanum lycopersicum l.), although no studies have extensively surveyed single nucleotide polymorphism (snp) diversity among tomato landraces. we estimated intraspecific dna sequence variation by analyzing 50 gene fragments (23.2 kb) per plant in a 31 plant diversity panel. the majority of loci (80%) were polymorphic with the minor allele at a frequency of 10% or less for most (141 of 155) snps. mean diversity as estimated by theta and p ... | 2009 | 19436327 |
| mineral and trace elements content in 30 accessions of tomato fruits (solanum lycopersicum l.,) and wild relatives (solanum pimpinellifolium l., solanum cheesmaniae l. riley, and solanum habrochaites s. knapp & d.m. spooner). | tomato quality and its potential health benefits are directly related to its chemical composition. the characterization of nutritional properties of solanum germplasm is essential to choose suitable donor parents for breeding programs. in this sense, wild species could be very useful for tomato fruit quality genetic improvement. with this objective, in this work, we characterize micronutrients content in eulycopersicon germplasm (20 cultivars of s. lycopersicum l. and 10 accessions of wild relat ... | 2011 | 20526750 |
| the identification of a gene (cwp1), silenced during solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit. | one of the most intriguing phenomena of fleshy fruit is the ability to maintain high water content at maturity, even following harvest. this is accomplished by a fruit cuticle that is highly impermeable to water diffusion. in this paper, we report on a novel genotype of tomato, developed via introgression from the wild species solanum habrochaites, which is characterized by microfissuring of the fruit cuticle and dehydration of the mature fruit. the microfissure/dehydration phenotype is inherite ... | 2007 | 17877702 |
| ripening behavior of wild tomato species. | nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ... | 1981 | 16662121 |
| dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics. | self-incompatibility (si), a phenomenon that is widespread among flowering plants (angiosperms), promotes outbreeding, resulting in increased genetic diversity and species survival. si is also important in establishing intra- or interspecies reproductive barriers, such as those that are evident in the tomato clade, solanum section lycopersicon, where they limit the use of wild species inbreeding programs to improve cultivated tomato. however, the molecular mechanisms underlying si are poorly und ... | 2016 | 27163628 |
| dissection of the style's response to pollination using transcriptome profiling in self-compatible (solanum pimpinellifolium) and self-incompatible (solanum chilense) tomato species. | tomato (solanum lycopersicum) self-compatibility (sc) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. self-incompatibility (si) is defined as self-pollen tubes that are prevented from developing in the style. to determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of sc and si tomato unpollinated/pollinated styles was performed using rna-sequencing (rna-seq) data. | 2015 | 25976872 |
| metabolomic analysis of tomato seed germination. | seed germination is inherently related to seed metabolism, which changes throughout its maturation, desiccation and germination processes. the metabolite content of a seed and its ability to germinate are determined by underlying genetic architecture and environmental effects during development. | 2017 | 29104520 |
| effective enhancement of resistance to phytophthora infestans by overexpression of mir172a and b in solanum lycopersicum. | overexpression of mir172a and b in tomato ( solanum lycopersicum ) zaofen no. 2 increased resistance to phytophthora infestans infection by suppressing of an ap2/erf transcription factor. the mir172 family has been shown to participate in the growth phase transition, flowering time control, abiotic and biotic stresses by regulating the expression of a small group of ap2/erf transcription factors. in this study, the precursors of mir172a and b were cloned from tomato, solanum pimpinellifolium l37 ... | 2018 | 28884358 |
| differential dna methylation and gene expression in reciprocal hybrids between solanum lycopersicum and s. pimpinellifolium. | wide hybridization is a common and efficient breeding strategy for enhancing crop yield and quality. an interesting phenomenon is that the reciprocal hybrids usually show different phenotypes, and its underlying mechanism is not well understood. here, we reported our comparative analysis of the dna methylation patterns in solanum lycopersicum, solanum pimpinellifolium and their reciprocal hybrids by methylated dna immunoprecipitation sequencing. the reciprocal hybrids had lower levels of dna met ... | 2017 | 28679169 |
| qtl mapping of fruit mineral contents provides new chances for molecular breeding of tomato nutritional traits. | agronomical characterization of a ril population for fruit mineral contents allowed for the identification of qtl controlling these fruit quality traits, flanked by co-dominant markers useful for marker-assisted breeding. tomato quality is a multi-variant attribute directly depending on fruit chemical composition, which in turn determines the benefits of tomato consumption for human health. commercially available tomato varieties possess limited variability in fruit quality traits. wild species, ... | 2017 | 28280866 |
| root-to-shoot hormonal communication in contrasting rootstocks suggests an important role for the ethylene precursor aminocyclopropane-1-carboxylic acid in mediating plant growth under low-potassium nutrition in tomato. | selection and breeding of rootstocks that can tolerate low k supply may increase crop productivity in low fertility soils and reduce fertilizer application. however, the underlying physiological traits are still largely unknown. in this study, 16 contrasting recombinant inbred lines (rils) derived from a cross between domestic and wild tomato species (solanum lycopersicum × solanum pimpinellifolium) have been used to analyse traits related to the rootstock-mediated induction of low (l, low shoot ... | 2016 | 27965690 |
| distribution, position and genomic characteristics of crossovers in tomato recombinant inbred lines derived from an interspecific cross between solanum lycopersicum and solanum pimpinellifolium. | we determined the crossover (co) distribution, frequency and genomic sequences involved in interspecies meiotic recombination by using parent-assigned variants of 52 f6 recombinant inbred lines obtained from a cross between tomato, solanum lycopersicum, and its wild relative, solanum pimpinellifolium. the interspecific co frequency was 80% lower than reported for intraspecific tomato crosses. we detected regions showing a relatively high and low co frequency, so-called hot and cold regions. cold ... | 2017 | 27797425 |
| ssr mapping of qtls conferring cold tolerance in an interspecific cross of tomato. | a population of 146 rils (recombinant inbred line) was derived from the cross between a cold-sensitive cultivated solanum lycopersicum l. xf98-7 and a cold-tolerant wild solanum pimpinellifolium la2184. relative germination ratio (rgr) and chilling index (ci) were used to evaluate the cold tolerance of the parental lines and rils. it was found that the rgr and ci were significantly different between s. lycopersicum xf98-7 and s. pimpinellifolium la2184 under cold treatment, indicating that wild ... | 2016 | 27517040 |
| fine mapping of the dialytic gene that controls multicellular trichome formation and stamen development in tomato. | using map-based cloning, we delimited the dialytic gene to an approximately 109-kb fragment, which controls multicellular trichome formation and stamen development in tomato. trichomes exist in the epidermis of nearly all terrestrial plants, including unicellular and multicellular types. the molecular mechanism of unicellular trichomes in arabidopsis is well characterized. however, knowledge about the regulatory pathway of multicellular trichomes in tomato (solanum lycopersicum) is limited. phen ... | 2016 | 27151537 |
| rna sequencing analysis of gene expression regulated by the transcription factor slzfp2 during early fruit development. | the transcription factor slzfp2 (solanum lycopersicum zinc finger protein 2) regulates aba biosynthesis during fruit development. to reveal the regulatory network of this transcription factor, we conducted a high-throughput rna-seq to identify differentially expressed genes in 2 dpa (days post anthesis) fruits from a representative rnai line in solanum pimpinellifolium la1589 background and the wild type. the transcriptome analysis revealed that expression of 2722 genes was regulated by slzfp2 d ... | 2016 | 27114906 |
| continuous light increases growth, daily carbon gain, antioxidants, and alters carbohydrate metabolism in a cultivated and a wild tomato species. | cultivated tomato species develop leaf injury while grown in continuous light (cl). growth, photosynthesis, carbohydrate metabolism and antioxidative enzyme activities of a cultivated (solanum lycopersicum l. 'aromata') and a wild tomato species (solanum pimpinellifolium l.) were compared in this study aiming to analyze the species-specific differences and thermoperiod effects in responses to cl. the species were subjected to three photoperiodic treatments for 12 days in climate chambers: 16-h p ... | 2015 | 26217371 |
| mapping in the era of sequencing: high density genotyping and its application for mapping tylcv resistance in solanum pimpinellifolium. | a ril population between solanum lycopersicum cv. moneymaker and s. pimpinellifolium g1.1554 was genotyped with a custom made snp array. additionally, a subset of the lines was genotyped by sequencing (gbs). | 2014 | 25526885 |
| genome-wide association in tomato reveals 44 candidate loci for fruit metabolic traits. | genome-wide association studies have been successful in identifying genes involved in polygenic traits and are valuable for crop improvement. tomato (solanum lycopersicum) is a major crop and is highly appreciated worldwide for its health value. we used a core collection of 163 tomato accessions composed of s. lycopersicum, s. lycopersicum var cerasiforme, and solanum pimpinellifolium to map loci controlling variation in fruit metabolites. fruits were phenotyped for a broad range of metabolites, ... | 2014 | 24894148 |
| preservation of solanum pimpinellifolium genomic fragments in recombinant genotypes improved the fruit quality of tomato. | five recombinant inbred lines obtained from the f2 generation of an interspecific cross between cultivar, caimanta (cai, solanum lycopersicum) and wild accession, la722 (p, s. pimpinellifolium) were crossed to obtain the second cycle hybrids (sch). eleven fruit quality traits were assessed in evaluating phenotypic variability among genotypes p, cai, f1 (cai x p), five rils, and 10 sch. one of the five recombinant inbred lines and three sch had higher values than p, as the best genotype for shelf ... | 2013 | 23970075 |
| phenotypic diversity and association mapping for fruit quality traits in cultivated tomato and related species. | association mapping has been proposed as an efficient approach to assist in the identification of the molecular basis of agronomical traits in plants. for this purpose, we analyzed the phenotypic and genetic diversity of a large collection of tomato accessions including 44 heirloom and vintage cultivars (solanum lycopersicum), 127 s. lycopersicum var. cerasiforme (cherry tomato) and 17 solanum pimpinellifolium accessions. the accessions were genotyped using a snplex™ assay of 192 snps, among whi ... | 2013 | 23124430 |
| variation revealed by snp genotyping and morphology provides insight into the origin of the tomato. | tomato, solanum lycopersicum, is divided into two widely distributed varieties: the cultivated s. lycopersicum var. lycopersicum, and the weedy s. lycopersicum var. cerasiforme. solanum pimpinellifolium is the most closely related wild species of tomato.the roles of s. pimpinellifolium and s. l. cerasiforme during the domestication of tomato are still under debate. some authors consider s. l. cerasiforme to be the ancestor, whereas others think that s. l. cerasiforme is an admixture of s. pimpin ... | 2012 | 23118951 |
| exploring the natural variation for seedling traits and their link with seed dimensions in tomato. | the success of germination, growth and final yield of every crop depends to a large extent on the quality of the seeds used to grow the crop. seed quality is defined as the viability and vigor attribute of a seed that enables the emergence and establishment of normal seedlings under a wide range of environments. we attempt to dissect the mechanisms involved in the acquisition of seed quality, through a combined approach of physiology and genetics. to achieve this goal we explored the genetic var ... | 2012 | 22952841 |
| the tomato genome sequence provides insights into fleshy fruit evolution. | tomato (solanum lycopersicum) is a major crop plant and a model system for fruit development. solanum is one of the largest angiosperm genera and includes annual and perennial plants from diverse habitats. here we present a high-quality genome sequence of domesticated tomato, a draft sequence of its closest wild relative, solanum pimpinellifolium, and compare them to each other and to the potato genome (solanum tuberosum). the two tomato genomes show only 0.6% nucleotide divergence and signs of ... | 2012 | 22660326 |
| complex genetics controls natural variation among seed quality phenotypes in a recombinant inbred population of an interspecific cross between solanum lycopersicum × solanum pimpinellifolium. | seed quality in tomato is associated with many complex physiological and genetic traits. while plant processes are frequently controlled by the action of small- to large-effect genes that follow classic mendelian inheritance, our study suggests that seed quality is primarily quantitative and genetically complex. using a recombinant inbred line population of solanum lycopersicum × solanum pimpinellifolium, we identified quantitative trait loci (qtls) influencing seed quality phenotypes under non- ... | 2012 | 22074055 |
| sun regulates vegetative and reproductive organ shape by changing cell division patterns. | one of the major genes controlling the elongated fruit shape of tomato (solanum lycopersicum) is sun. in this study, we explored the roles of sun in vegetative and reproductive development using near isogenic lines (nils) that differ at the sun locus, and sun overexpressors in both the wild species la1589 (solanum pimpinellifolium) and the cultivar sun1642 background. our results demonstrate that sun controls tomato shape through redistribution of mass that is mediated by increased cell division ... | 2011 | 21921117 |
| phenotypic and molecular characterization of selected tomato recombinant inbred lines derived from the cross solanum lycopersicum x s. pimpinellifolium. | an important trait defining fresh tomato marketability is fruit shelf life. exotic germplasm of solanum pimpinellifolium is able to prolong shelf life. sixteen recombinant inbred lines with differing values of shelf life and fruit weight were derived by antagonistic-divergent selection from an interspecific cross involving solanum pimpinellifolium. the objective of this study was to evaluate these recombinant inbred lines for many fruit quality traits such as diameter, height, size, acidity, col ... | 2011 | 21869471 |
| comparative transcriptomic profiling of a salt-tolerant wild tomato species and a salt-sensitive tomato cultivar. | wild halophytic tomato has long been considered as an ideal gene donor for improving salt tolerance in tomato cultivars. extensive research has been focused on physiological and quantitative trait locus (qtl) characterization of wild tomato species in comparison with cultivated tomato. however, the global gene expression modification of wild tomato in response to salt stress is not well known. a wild tomato genotype, solanum pimpinellifolium 'pi365967' is significantly more salt tolerant than th ... | 2010 | 20410049 |
| a new genetic linkage map of tomato based on a solanum lycopersicum x s. pimpinellifolium ril population displaying locations of candidate pathogen response genes. | the narrow genetic base of the cultivated tomato, solanum lycopersicum l., necessitates introgression of new variation from related species. wild tomato species represent a rich source of useful genes and traits. exploitation of genetic variation within wild species can be facilitated by the use of molecular markers and genetic maps. recently we identified an accession (la2093) within the red-fruited wild tomato species solanum pimpinellifolium l. with exceptionally desirable characteristics, in ... | 2009 | 19935918 |
| resistance to the two-spotted spider mite (tetranychus urticae) by acylsucroses of wild tomato (solanum pimpinellifolium) trichomes studied in a recombinant inbred line population. | trichome-based host plant resistance is a complex mechanism that could be used in tomato breeding to control arthropod pests. the aims of this work were to evaluate the plant traits (density of trichomes and acylsucrose production) and the functional relationships of these traits with mortality, repellence, and oviposition of tetranychus urticae koch (acari: tetranychidae). we used a population of recombinant inbred lines (rils) derived from the cross between the wild tomato, solanum pimpinellif ... | 2009 | 18807203 |
| diversity in conserved genes in tomato. | tomato has excellent genetic and genomic resources including a broad set of expressed sequence tag (est) data and high-density genetic maps. in addition, emerging physical maps and bacterial artificial clone sequence data serve as template to investigate genetic variation within the cultivated germplasm pool with the goal to manipulate agriculturally important traits. unfortunately, the nearly exclusive focus of resource development on interspecific populations for genetic analyses and diversity ... | 2007 | 18088428 |
| genetics of drought tolerance during seed germination in tomato: inheritance and qtl mapping. | a bc1 population (n = 1000) of an f1 hybrid between a stress-sensitive lycopersicon esculentum breeding line (nc84173; maternal and recurrent parent) and a germination stress-tolerant lycopersicon pimpinellifolium accession (la722) was evaluated for seed germination rate under drought stress (ds) (14% w/v polyethyleneglycol-8000, water potential approximately -680 kpa), and the most rapidly germinating seeds (first 3% to germinate) were selected. the 30 selected bc1 seedlings were grown to matur ... | 2003 | 12897861 |
| extremely elongated tomato fruit controlled by four quantitative trait loci with epistatic interactions. | cultivated tomato ( lycopersicon esculentum) encompass a wide range of fruit shape and size variants. this variation can be used to genetically dissect the molecular basis of ovary and fruit morphology. the cultivar long john displays an extremely elongated fruit phenotype, while the wild relative lycopersicon pimpinellifolium la1589 produces fruit that are nearly perfect spheres, typical of wild tomatoes. quantitative trait mapping of an f2 population between long john and la1589 revealed four ... | 2002 | 12582693 |
| mapping quantitative trait loci in inbred backcross lines of lycopersicon pimpinellifolium (la1589). | although tomato has been the subject of extensive quantitative trait loci (qtls) mapping experiments, most of this work has been conducted on transient populations (e.g., f2 or backcross) and few homozygous, permanent mapping populations are available. to help remedy this situation, we have developed a set of inbred backcross lines (ibls) from the interspecific cross between lycopersicon esculentum cv. e6203 and l. pimpinellifolium (la1589). a total of 170 bc2f1 plants were selfed for five gener ... | 2002 | 12502266 |
| relationship between tomato fruit growth and fruit osmotic potential under salinity. | to investigate the relationship between fruit growth and fruit osmotic potential (psi(s)) in salty conditions, a sensitive tomato cultivar (lycopersicon esculentum mill.) and a tolerant accession of the wild species lycopersicon pimpinellifolium mill. were grown in a greenhouse with 0 and 70 mm nacl, and the growth of the fruit studied from 15 to 70 days after anthesis (daa). l. pimpinellifolium did not reduce significantly fruit weight in salty conditions throughout the growth period, whereas l ... | 2001 | 11337072 |
| a putative role for the tomato genes dumpy and curl-3 in brassinosteroid biosynthesis and response. | the dumpy (dpy) mutant of tomato (lycopersicon esculentum mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. application of brassinolide and castasterone rescued the dpy phenotype, as did c-23-hydroxylated, 6-deoxo intermediates of brassinolide biosynthesis. the brassinolide precursors campesterol, campestanol, and 6-deoxocathasterone failed to rescue, suggesting that dpy may be affected in the conversion of 6-deoxocathasterone to 6-deoxoteasterone, similar t ... | 2000 | 10631252 |
| definition of nonvolatile markers for flavor of tomato (lycopersicon esculentum mill.) as tools in selection and breeding. | a methodology for flavor and composition assessment of processed tomato juice samples was developed using a wide range of commercial processing tomato varieties (lycopersicon esculentum) grown in spain and the united states. fruitiness intensity was found by a trained panel to best describe overall tomato flavor. for two consecutive years, fruitiness intensity was significantly dependent on growing location and variety, and it was consistently linked to increased levels of glucose and reducing s ... | 1999 | 10563949 |
| analysis of mrnas that accumulate in response to low temperature identifies a thiol protease gene in tomato. | we have studied the induction of gene expression at low temperature by cloning mrnas that accumulate when unripe tomato (lycopersicon esculentum) fruit are incubated at 4 degrees c. two cloned mrnas, c14 and c17, accumulate relatively rapidly in response to cold treatment, while a third, c19, displays a delayed response. significant levels of these mrnas were not detected during fruit ripening at normal temperature. we have analyzed gene expression at different temperatures and detect half-maxim ... | 1988 | 16666159 |
| linkage analysis of quantitative traits in an interspecific cross of tomato (lycopersicon esculentum x lycopersicon pimpinellifolium) by means of genetic markers. | linkage relationships between loci affecting quantitative traits (qtl) and marker loci were examined in an interspecific cross between lycopersicon esculentum and lycopersicon pimpinellifolium. parental lines differed for six morphological markers and for four electrophoretic markers. almost 1700 f-2 plants were scored with respect to the genetic markers and also with respect to 18 quantitative traits. major genes affecting the quantitative traits were not found, but out of 180 possible marker x ... | 1988 | 17246412 |
| transcriptome signatures of tomato leaf induced by phytophthora infestans and functional identification of transcription factor spwrky3. | spwrky3 was identified as a resistance gene to phytophthora infestans from solanum pimpinellifolium l3708 and its transgenic tomato showed a significant resistance to p. infestans. this finding reveals the potential application of spwrky3 in future molecular breeding. transcription factors (tfs) play crucial roles in the plant response to various pathogens. in this present study, we used comparative transcriptome analysis of tomatoes inoculated with and without phytophthora infestans to identify ... | 2017 | 29234827 |
| whole-genome re-sequencing of two italian tomato landraces reveals sequence variations in genes associated with stress tolerance, fruit quality and long shelf-life traits. | tomato is a high value crop and the primary model for fleshy fruit development and ripening. breeding priorities include increased fruit quality, shelf life and tolerance to stresses. to contribute towards this goal, we re-sequenced the genomes of corbarino (cor) and lucariello (luc) landraces, which both possess the traits of plant adaptation to water deficit, prolonged fruit shelf-life and good fruit quality. through the newly developed pipeline reconstructor, we generated the genome sequences ... | 2017 | 29149280 |
| genetic analysis of rootstock-mediated nitrogen (n) uptake and root-to-shoot signalling at contrasting n availabilities in tomato. | selecting rootstocks for high nitrogen acquisition ability may allow decreased n fertilizer application without reducing tomato yields, minimizing environmental nitrate pollution. a commercial hybrid tomato variety was grafted on a genotyped population of 130 recombinant inbred lines (rils) derived from solanum pimpinellifolium, and compared with self- and non-grafted controls under contrasting nitrate availabilities (13.8 vs 1.0mm) in the nutrient solution. grafting itself altered xylem sap com ... | 2017 | 28818388 |
| genome-wide snp discovery and qtl mapping for fruit quality traits in inbred backcross lines (ibls) of solanum pimpinellifolium using genotyping by sequencing. | solanum pimpinellifolium has high breeding potential for fruit quality traits and has been used as a donor in tomato breeding programs. unlocking the genetic potential of s. pimpinellifolium requires high-throughput polymorphism identification protocols for qtl mapping and introgression of favourable alleles into cultivated tomato by both positive and background selection. | 2017 | 28049423 |
| improving agronomic water use efficiency in tomato by rootstock-mediated hormonal regulation of leaf biomass. | water availability is the most important factor limiting food production, thus developing new scientific strategies to allow crops to more efficiently use water could be crucial in a world with a growing population. tomato is a highly water consuming crop and improving its water use efficiency (wue) implies positive economic and environmental effects. this work aimed to study and exploit root-derived hormonal traits to improve wue in tomato by grafting on selected rootstocks. firstly, root-relat ... | 2016 | 27593467 |
| [effects of day and night temperature difference on growth, development, yield and fruit quality of tomatoes]. | abstract: the effects of day and night temperature difference (dif) on tomato's growth were studied in three precisely controlled units in phytotron. set dif as 6 °c (25/19 c), 8 °c (26/18 °c), 10 °c (27/17 °c) respectively, with the same diurnal mean temperature as 22 °c. the results showed that, different tomato varieties needed different suitable dif at different growth stages. before flouring, compared with dif 6 °c , dif 8 °c could significantly improve the growth and development of the wil ... | 2015 | 26785551 |
| the control of tomato fruit elongation orchestrated by sun, ovate and fs8.1 in a wild relative of tomato. | within the cultivated tomato germplasm, sun, ovate and fs8.1 are the three predominant qtls controlling fruit elongation. although sun and ovate have been cloned, their role in plant growth and development are not well understood. to compare and contrast the effects of the three qtls in a homogeneous background, we developed near isogenic lines (nils) in the wild species solanum pimpinellifolium la1589 background. we carried out detailed morphological characterization of reproductive and vegetat ... | 2015 | 26259178 |
| wide-genome qtl mapping of fruit quality traits in a tomato ril population derived from the wild-relative species solanum pimpinellifolium l. | qtl and candidate genes associated to fruit quality traits have been identified in a tomato genetic map derived from solanum pimpinellifolium l., providing molecular tools for marker-assisted breeding. the study of genetic, physiological, and molecular pathways involved in fruit development and ripening has considered tomato as the model fleshy-fruited species par excellence. fruit quality traits regarding organoleptic and nutritional properties are major goals for tomato breeding programs since ... | 2015 | 26163766 |
| variations in dreb1a and vp1.1 genes show association with salt tolerance traits in wild tomato (solanum pimpinellifolium). | association analysis was conducted in a core collection of 94 genotypes of solanum pimpinellifolium to identify variations linked to salt tolerance traits (physiological and yield traits under salt stress) in four candidate genes viz., dreb1a, vp1.1, nhx1, and tip. the candidate gene analysis covered a concatenated length of 4594 bp per individual and identified five snp/indels in dreb1a and vp1.1 genes explaining 17.0% to 25.8% phenotypic variation for various salt tolerance traits. out of thes ... | 2015 | 26161546 |
| genetic dissection of tomato rootstock effects on scion traits under moderate salinity. | rootstock hkt1 genotype affected fruit [na(+)] and non-commercial fruit yield; qtl analysis of rootstock-mediated scion nutrition is a powerful forward genetic approach to identify wild genes for rootstock breeding. the present study approaches the qtl dissection of rootstock effects on a commercial hybrid variety grafted on a population of rils derived from solanum pimpinellifolium, genotyped for 4370 segregating snps from the solcap tomato panel and grown under moderate salinity. results are c ... | 2015 | 25628165 |
| functional genomics of tomato: opportunities and challenges in post-genome ngs era. | the tomato genome sequencing project represented a landmark venture in the history of sequencing projects where both sanger's and next-generation sequencing (ngs) technologies were employed, and a highly accurate and one of the best assembled plant genomes along with a draft of the wild relative, solanum pimpinellifolium, were released in 2012. however, the functional potential of the major portion of this newly generated resource is still undefined. the very first challenge before scientists wo ... | 2014 | 25431420 |
| genetic mapping of two qtl from the wild tomato solanum pimpinellifolium l. controlling resistance against two-spotted spider mite (tetranychus urticae koch). | a novel source of resistance to two-spotted spider mite (tetranychus urticae koch) was found in solanum pimpinellifolium l. accession to-937 and thereby a potential source of desirable traits that could be introduced into new tomato varieties. this resistance was found to be controlled by a major locus modulated by minor loci of unknown location in the genome of this wild tomato. we first applied a bulked segregant analysis (bsa) approach in an f(4) population as a method for rapidly identifying ... | 2013 | 22903693 |
| [salt resistance of different tomato genotypes at seed germination stage]. | based on the cluster analysis of relative germination potential and relative germination rate under nacl stress, fourteen tomato genotypes were clustered into two groups. five tomato genotypes held high resistance to nacl stress, and nine tomato genotypes were moderately nacl- tolerant. the highly nacl- tolerant genotypes wild lycopersicon pimpinellifolium, liaoyuanhongmanao and hongbaoshi and the moderately nacl- tolerant genotype liaoyuanhongduoli were then under different na(+)-salts and nacl ... | 2009 | 19899472 |
| integration of tomato reproductive developmental landmarks and expression profiles, and the effect of sun on fruit shape. | universally accepted landmark stages are necessary to highlight key events in plant reproductive development and to facilitate comparisons among species. domestication and selection of tomato resulted in many varieties that differ in fruit shape and size. this diversity is useful to unravel underlying molecular and developmental mechanisms that control organ morphology and patterning. the tomato fruit shape gene sun controls fruit elongation. the most dramatic effect of sun on fruit shape occurs ... | 2009 | 19422692 |