| response of tomato and its wild relatives in the genus solanum to cucumber mosaic virus and satellite rna combinations. | the differential response of 29 genotypes of tomato and wild tomato relatives (solanum section lycopersicon species) to cucumber mosaic virus strain fny (cmv-fny), alone or in combination with three different satellite rna (satrna) variants, allowed the identification of four disease phenotype patterns, each including plants that developed very severe symptoms (leaf malformations, top stunting and lethal necrosis) and plants that remained asymptomatic. no resistance or tolerance to cmv-fny was o ... | 2007 | 17947544 |
| why to account for finite sites in population genetic studies and how to do this with jaatha 2.0. | with the advent of next-generation sequencing technologies, large data sets of several thousand loci from multiple conspecific individuals are available. such data sets should make it possible to obtain accurate estimates of population genetic parameters, even for complex models of population history. in the analyses of large data sets, it is difficult to consider finite-sites mutation models (fsms). here, we use extensive simulations to demonstrate that the inclusion of fsms is necessary to avo ... | 2013 | 24198930 |
| mapping and introgression of a tomato yellow leaf curl virus tolerance gene, ty-1. | the whitefly-transmitted tomato yellow-leaf curl gemini-virus (tylcv) is a major pathogen of tomatoes. the wild tomato species lycopersicon chilense, which is resistant to the virus, was crossed to the cultivated tomato, l. esculentum. the backcross-1 selfed (bc1s1) generation was inoculated and a symptomless plant was selected. this plant was analyzed using 61 molecular markers, which span the tomato genome, to determine which l. chilense chromosome segments were introgressed. a bc2s1 populatio ... | 1994 | 24185918 |
| assessing the genetic variation of ty-1 and ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm. | tomato yellow leaf curl virus (tylcv) hampers tomato production worldwide. our previous studies have focussed on mapping and ultimately cloning of the tylcv resistance genes ty-1 and ty-3. both genes are derived from solanum chilense and were shown to be allelic. they code for an rna-dependent rna polymerase (rdr) belonging to the rdrγ type defined by a dfdgd catalytic domain. in this study, we first fine-mapped the tylcv resistance in s. chilense la1932, la1960 and la1971. results showed that c ... | 2017 | 26028987 |
| the tomato yellow leaf curl virus resistance genes ty-1 and ty-3 are allelic and code for dfdgd-class rna-dependent rna polymerases. | tomato yellow leaf curl virus disease incited by tomato yellow leaf curl virus (tylcv) causes huge losses in tomato production worldwide and is caused by different related begomovirus species. breeding for tylcv resistance has been based on the introgression of multiple resistance genes originating from several wild tomato species. in this study we have fine-mapped the widely used solanum chilense-derived ty-1 and ty-3 genes by screening nearly 12,000 plants for recombination events and generati ... | 2013 | 23555305 |
| identification of resistance to meloidogyne javanica in the lycopersicon peruvianum complex. | clones of lycopersicon peruvianum pi 2704352r2, pi 270435-3mh and pi 126443-1mh expressed novel resistance to three mi-avirulent m. javanica isolates in greenhouse experiments. clones from pi 126443-1mh were resistant to the three m. javanica isolates at 25°c. the three isolates were able to reproduce on one embryorescue hybrid of pi 126443-1mh, but not on three l. peruvianum-l. esculentum bridge-line hybrids of pi 1264431mh when screened at 25°c (mi-expressed temperature). clones of pi 270435-2 ... | 1996 | 24162423 |
| chromosomal rearrangements between tomato and solanum chilense hamper mapping and breeding of the tylcv resistance gene ty-1. | tomato yellow leaf curl disease, a devastating disease of solanum lycopersicum (tomato), is caused by a complex of begomoviruses generally referred to as tomato yellow leaf curl virus (tylcv). almost all breeding for tylcv resistance has been based on the introgression of the ty-1 resistance locus derived from solanum chilense la1969. knowledge about the exact location of ty-1 on tomato chromosome 6 will help in understanding the genomic organization of the ty-1 locus. in this study, we analyze ... | 2011 | 21883550 |
| anthocyanin1 from solanum chilense is more efficient in accumulating anthocyanin metabolites than its solanum lycopersicum counterpart in association with the anthocyanin fruit phenotype of tomato. | anthocyanins are flavonoid metabolites contributing attractive colors and antioxidant qualities to the human diet. accordingly, there is a growing interest in developing crops enriched with these compounds. fruits of the cultivated tomato, solanum (s.) lycopersicum, do not normally produce high levels of anthocyanins. however, several wild tomato species yield anthocyanin-pigmented fruits, and this trait has been introgressed into the cultivated tomato. two genes encoding homologous r2r3 myb tra ... | 2011 | 21947299 |
| involvement of ethylene in stress-induced expression of the tlc1.1 retrotransposon from lycopersicon chilense dun. | the tlc1 family is one of the four families of long terminal repeat (ltr) retrotransposons identified in the genome of lycopersicon chilense. here, we show that this family of retroelements is transcriptionally active and its expression is induced in response to diverse stress conditions such as wounding, protoplast preparation, and high salt concentrations. several stress-associated signaling molecules, including ethylene, methyl jasmonate, salicylic acid, and 2,4-dichlorophenoxyacetic acid, ar ... | 2005 | 16040666 |
| isolation of an osmotic stress- and abscisic acid-induced gene encoding an acidic endochitinase from lycopersicon chilense. | we have identified one osmotic stress- and abscisic acid-responsive member of the endochitinase (ec 3.2.1.14) gene family from leaves of drought-stressed lycopersicon chilense plants, a natural inhabitant of extremely arid regions in south america. the 966-bp full-length cdna (designated pcht28) encodes an acidic chitinase precursor with an amino-terminal signal peptide. the mature protein is predicted to have 229 amino acid residues with a relative molecular mass of 24,943 and pi value of 6.2. ... | 1994 | 7816027 |
| the wild tomato species solanum chilense shows variation in pathogen resistance between geographically distinct populations. | wild tomatoes are a valuable source of disease resistance germplasm for tomato (solanum lycopersicum) breeders. many species are known to possess a certain degree of resistance against certain pathogens; however, evolution of resistance traits is yet poorly understood. for some species, like solanum chilense, both differences in habitat and within species genetic diversity are very large. here we aim to investigate the occurrence of spatially heterogeneous coevolutionary pressures between popula ... | 2017 | 28133579 |
| phytohormone profiling in relation to osmotic adjustment in nacl-treated plants of the halophyte tomato wild relative species solanum chilense comparatively to the cultivated glycophyte solanum lycopersicum. | a holistic approach was used to investigate the hormonal profile in relation with osmotic adjustment under salinity in solanum lycopersicum and its halophyte wild relative solanum chilense. plants were subjected to 125mm nacl for 7days. solanum chilense displayed a contrasting behaviour comparatively to s. lycopersicum, not only for mineral nutrition, but also regarding the modalities of osmotic adjustment and phytohormonal profiling. the extent of osmotic adjustment was higher in s. chilense th ... | 0 | 28330565 |
| the dominant allele aft induces a shift from flavonol to anthocyanin production in response to uv-b radiation in tomato fruit. | the introgression of the a ft allele into domesticated tomato induced a shift from flavonol to anthocyanin production in response to uv-b radiation, while the hp - 1 allele negatively influenced the response of flavonoid biosynthesis to uv-b. introgression of the dominant allele anthocyanin fruit (aft) from solanum chilense induces anthocyanin accumulation in the peel of tomato (solanum lycopersicum l.) fruit. uv-b radiation can influence plant secondary metabolism regulating the expression of s ... | 0 | 28516293 |
| engineered drought-induced biosynthesis of α-tocopherol alleviates stress-induced leaf damage in tobacco. | tocopherols are members of the vitamin e complex and essential antioxidant compounds synthesized in chloroplasts that protect photosynthetic membranes against oxidative damage triggered by most environmental stresses. tocopherol deficiency has been shown to affect germination, retard growth and change responses to abiotic stress, suggesting that tocopherols may be involved in a number of diverse physiological processes in plants. instead of seeking constitutive synthesis of tocopherols to improv ... | 2013 | 23651908 |
| the sbasr-1 gene cloned from an extreme halophyte salicornia brachiata enhances salt tolerance in transgenic tobacco. | salinity severely affects plant growth and development. plants evolved various mechanisms to cope up stress both at molecular and cellular levels. halophytes have developed better mechanism to alleviate the salt stress than glycophytes, and therefore, it is advantageous to study the role of different genes from halophytes. salicornia brachiata is an extreme halophyte, which grows luxuriantly in the salty marshes in the coastal areas. earlier, we have isolated sbasr-1 (abscisic acid stress ripeni ... | 2012 | 22639284 |
| plastidic isoprenoid biosynthesis in tomato: physiological and molecular analysis in genotypes resistant and sensitive to drought stress. | isoprenoid compounds synthesised in the plastids are involved in plant response to water deficit. the functionality of the biosynthetic pathway of these compounds under drought stress has been analysed at the physiological and molecular levels in two related species of tomato (solanum chilense and solanum lycopersicum) that differ in their tolerance to abiotic challenge. expression analysis of the genes encoding enzymes of these pathways (dxs, ipi, ggpps, psy1, nced and hpt1) in plants at differ ... | 2012 | 21974688 |
| inference of seed bank parameters in two wild tomato species using ecological and genetic data. | seed and egg dormancy is a prevalent life-history trait in plants and invertebrates whose storage effect buffers against environmental variability, modulates species extinction in fragmented habitats, and increases genetic variation. experimental evidence for reliable differences in dormancy over evolutionary scales (e.g., differences in seed banks between sister species) is scarce because complex ecological experiments in the field are needed to measure them. to cope with these difficulties, we ... | 2011 | 21949404 |
| jaatha: a fast composite-likelihood approach to estimate demographic parameters. | while information about a species' demography is interesting in its own right, it is an absolute necessity for certain types of population genetic analyses. the most widely used methods to infer a species' demographic history do not take intralocus recombination or recent divergence into account, and some methods take several weeks to converge. here, we present jaatha, a new composite-likelihood method that does incorporate recent divergence and is also applicable when intralocus recombination r ... | 2011 | 21645157 |
| [process of meiosis in interspecific hybrid f1 lycopersicon esculentum mill. x lycopersicon chilense dun]. | the investigation concerns meiosis behaviour in embryo-culture-obtained lycopersicon esculentum mill. (mutant seedline mo 638) x l. chilense dun. f1 hybrid and its parental forms. it was determined that chiasma frequency decreased while univalent and meiotic disorder frequencies increased in f1 plants in comparison with parents forms. univalent number and the percent of main disorders lowered with bud tier increasing. the conclusion was made about meiosis regularity connection with the influence ... | 2011 | 21574426 |
| adaptation to drought in two wild tomato species: the evolution of the asr gene family. | wild tomato species are a valuable system in which to study local adaptation to drought: they grow in diverse environments ranging from mesic to extremely arid conditions. here, we investigate the evolution of members of the asr (aba/water stress/ripening induced) gene family, which have been reported to be involved in the water stress response. we analysed molecular variation in the asr gene family in populations of two closely related species, solanum chilense and solanum peruvianum. we conclu ... | 2011 | 21323928 |
| accumulation of acidic sk₃ dehydrins in phloem cells of cold- and drought-stressed plants of the solanaceae. | the role of acidic sk(n) dehydrins in stress tolerance of important crop and model species of the solanaceae remains unknown. we have previously shown that the acidic sk₃ dehydrin dhn24 from solanum sogarandinum is constitutively expressed and its expression is associated with cold acclimation. here we found that dhn24 is specifically localized to phloem cells of vegetative organs of non-acclimated plants. more precise localization of dhn24 revealed that it is primarily found in sieve elements ( ... | 2014 | 24395200 |
| studies of self-incompatibility in wild tomatoes: i. s-allele diversity in solanum chilense (dun.) reiche [corrected] (solanaceae). | we characterized the molecular allelic variation of rnases at the self-incompatibility (si) locus of solanum chilense dun. we recovered 30 s-rnase allele sequences from 34 plants representing a broad geographic sample. this yielded a species-wide estimate of 35 (95% likelihood interval 31-40) s-alleles. we performed crosses to confirm the association with si function of 10 of the putative s-rnase allele sequences. results in all cases were consistent with the expectation that these sequences rep ... | 2007 | 17700636 |
| north-south colonization associated with local adaptation of the wild tomato species solanum chilense. | after colonization population sizes may vary across the species range depending on environmental conditions and following colonizations. an interesting question is whether local adaptation occurs more frequently in large ancestral populations or in small derived populations. a higher number of new mutations and a lower effect of genetic drift should favor selection in large populations, whereas small derived populations may require an initial local adaptation event to facilitate the colonization ... | 2015 | 26232423 |
| different combinations of morpho-physiological traits are responsible for tolerance to drought in wild tomatoes solanum chilense and solanum peruvianum. | herbaceous species can modify leaf structure during the growing season in response to drought stress and water loss. evolution can select combinations of traits in plants for efficient water use in restricted environments. we investigated plant traits that mediate adaptation and acclimation to water stress in two herbaceous drought-tolerant species. anatomical, morphological and physiological traits related to stems and leaves were examined under optimal watering (ow) and a long period of restri ... | 2016 | 26499789 |
| adaptation to low temperatures in the wild tomato species solanum chilense. | molecular adaptation to abiotic stresses in plants is a complex process based mainly on the modifications of gene transcriptional activity and the alteration of protein-protein interactions. we used a combination of population genetic, comparative transcriptomic and plant physiology approaches to investigate the mechanisms of adaptation to low temperatures in solanum chilense populations distributed along andean altitudinal gradients. we found that plants from all populations have high chilling ... | 2016 | 27037798 |
| salicylic acid differently impacts ethylene and polyamine synthesis in the glycophyte solanum lycopersicum and the wild-related halophyte solanum chilense exposed to mild salt stress. | this study aimed to determine the effects of exogenous application of salicylic acid (sa) on the toxic effects of salt in relation to ethylene and polyamine synthesis, and to correlate these traits with the expression of genes involved in ethylene and polyamine metabolism in two tomato species differing in their sensitivity to salt stress, solanum lycopersicum cv ailsa craig and its wild salt-resistant relative solanum chilense. in s. chilense, treatment with 125 mm nacl improved plant growth, i ... | 2016 | 27105808 |
| dissecting stylar responses to self-pollination in wild tomato self-compatible and self-incompatible species using comparative proteomics. | self-incompatibility (si), a phenomenon that is widespread among flowering plants (angiosperms), promotes outbreeding, resulting in increased genetic diversity and species survival. si is also important in establishing intra- or interspecies reproductive barriers, such as those that are evident in the tomato clade, solanum section lycopersicon, where they limit the use of wild species inbreeding programs to improve cultivated tomato. however, the molecular mechanisms underlying si are poorly und ... | 2016 | 27163628 |
| inhibition of ethylene synthesis reduces salt-tolerance in tomato wild relative species solanum chilense. | exposure to salinity induces a burst in ethylene synthesis in the wild tomato halophyte plant species solanum chilense. in order to gain information on the role of ethylene in salt adaptation, plants of solanum chilense (accession la4107) and of cultivated glycophyte solanum lycopersicum (cv. ailsa craig) were cultivated for 7days in nutrient solution containing 0 or 125mm nacl in the presence or absence of the inhibitor of ethylene synthesis (aminovinylglycine (avg) 2μm). salt-induced ethylene ... | 2017 | 28040626 |
| involvement of schrabgdi1 from solanum chilense in endocytic trafficking and tolerance to salt stress. | physiological responses of plants to salinity stress requires the coordinated activation of many genes. a salt-induced gene was isolated from roots of the wild tomato species solanum chilense and named schrabgdi1 because it encodes a protein with high identity to gdp dissociation inhibitors of plants. these proteins are regulators of the rabgtpase cycle that play key roles in intracellular vesicular trafficking. the expression pattern of schrabgdi1 showed an early up-regulation in roots and leav ... | 2017 | 28818364 |
| population genomics in wild tomatoes-the interplay of divergence and admixture. | hybridization between closely related plant species is widespread, but the outcomes of hybridization are not fully understood. this study investigates phylogenetic relationships and the history of hybridization in the wild tomato clade (solanum sect. lycopersicon). we sequenced rna from individuals of 38 different populations and, by combining this with published data, build a comprehensive genomic data set for the entire clade. the data indicate that many taxa are not monophyletic and many indi ... | 2017 | 29077853 |
| ripening behavior of wild tomato species. | nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ... | 1981 | 16662121 |
| incidence and developmental timing of endosperm failure in post-zygotic isolation between wild tomato lineages. | defective hybrid seed development in angiosperms might mediate the rapid establishment of intrinsic post-zygotic isolation between closely related species. extensive crosses within and among three lineages of wild tomatoes (solanum section lycopersicon) were performed to address the incidence, developmental timing and histological manifestations of hybrid seed failure. these lineages encompass different, yet fairly recent, divergence times and both allopatric and partially sympatric pairs. | 2017 | 29280998 |
| using multilocus sequence data to assess population structure, natural selection, and linkage disequilibrium in wild tomatoes. | we employed a multilocus approach to examine the effects of population subdivision and natural selection on dna polymorphism in 2 closely related wild tomato species (solanum peruvianum and solanum chilense), using sequence data for 8 nuclear loci from populations across much of the species' range. both species exhibit substantial levels of nucleotide variation. the species-wide level of silent nucleotide diversity is 18% higher in s. peruvianum (pi(sil) approximately 2.50%) than in s. chilense ... | 2007 | 17675653 |
| the promoter of the tlc1.1 retrotransposon from solanum chilense is activated by multiple stress-related signaling molecules. | the ltr retrotransposons are the most abundant mobile elements in the plant genome and seem to play an important role in genome reorganization induced by environmental challenges. their success in this function depends on the ability of their promoters to respond to different signaling pathways that regulate plant adaptation to biotic and abiotic stresses. the promoter of the tlc1.1 retrotransposon from solanum chilense contains two primary ethylene-responsive elements (pere boxes) that are esse ... | 2007 | 17583815 |
| nucleotide polymorphism in the drought responsive gene asr2 in wild populations of tomato. | the asr gene family (named after abscicic acid [aba], stress, ripening), exclusively present in plant genomes, is involved in transcriptional regulation. its members are up-regulated in roots and leaves of water- or salt-stressed plants. in previous work, evidence of adaptive evolution (as inferred from synonymous and nonsynonymous divergence rates) has been reported for asr2 in solanum chilense and s. arcanum, two species dwelling in habitats with different precipitation regimes. in this paper ... | 2009 | 18636230 |
| dissecting the mechanism of solanum lycopersicum and solanum chilense flower colour formation. | flowers are the defining feature of angiosperms, and function as indispensable organs for sexual reproduction. flower colour typically plays an important role in attracting pollinators, and can show considerable variation, even between closely related species. for example, domesticated tomato (s. lycopersicum) has orange/yellow flowers, while the wild relative s. chilense (accession la2405) has bright yellow flowers. in this study, the mechanism of flower colour formation in these two species wa ... | 2015 | 24750468 |
| sequence evolution and expression regulation of stress-responsive genes in natural populations of wild tomato. | the wild tomato species solanum chilense and s. peruvianum are a valuable non-model system for studying plant adaptation since they grow in diverse environments facing many abiotic constraints. here we investigate the sequence evolution of regulatory regions of drought and cold responsive genes and their expression regulation. the coding regions of these genes were previously shown to exhibit signatures of positive selection. expression profiles and sequence evolution of regulatory regions of me ... | 2013 | 24205149 |
| a proline-, threonine-, and glycine-rich protein down-regulated by drought is localized in the cell wall of xylem elements. | a cdna clone encoding a proline-, threonine-, and glycine-rich protein (ptgrp) was isolated from a wild tomato species (lycopersicon chilense) (l.x. yu, h. chamberland, j.g. lafontain, z. tabaeizadeh [1996] genome 39: 1185-1193). northern-blot analysis and in situ hybridization studies revealed that ptgrp is down-regulated by drought stress. the level of the mrna in leaves and stems of 8-d drought-stressed plants decreased 5- to 10-fold compared with that in regularly watered plants. the mrna re ... | 1999 | 10517847 |
| highly heterogeneous families of ty1/copia retrotransposons in the lycopersicon chilense genome. | we have used the degenerated oligonucleotide primers-pcr (dop-pcr) technique to determine the presence of ty1/copia-related retrotransposons in the wild species of tomato, lycopersicon chilense. using degenerated oligonucleotides corresponding to highly conserved domains in the ty1/copia retrotransposons, fragments of roughly 300 bp were obtained by pcr amplification. these were cloned in a plasmid vector and the nucleotide sequence determined for 20 clones, 19 of which showed sequence homology ... | 1998 | 9831656 |
| negative regulation of gene expression of a novel proline-, threonine-, and glycine-rich protein by water stress in lycopersicon chilense. | we have isolated a full length cdna clone (designated ptgrp) encoding a proline-rich protein from leaves of lycopersicon chilense. sequence analysis of the 552-bp insert revealed that the open reading frame encodes a 12.6-kda protein. the deduced amino acid sequence of ptgrp consists of a c-terminal proline-rich domain with two identical repeat motifs phe-pro-met-pro-thr-thr-pro-ser-thr-gly-gly-gly-phe-pro-ser. the n terminus lacks proline and is hydrophobic. unlike other proline-rich proteins t ... | 1996 | 8983187 |
| transformation of the wild tomatolycopersicon chilense dun. byagrobacterium tumefaciens. | leaf disc transformation-regeneration technique was applied to the drought tolerant wild relative of cultivated tomato,lycopersicon chilense, using a plasmid construct which contained the coding sequences of neomycin phosphotransferase (nptii) and chloramphenicol acetyltransferase (cat) genes. the two genotypes used, la2747 and la1930, showed a distinct difference in their aptitude to transformation; a higher success rate was obtained for the first genotype in every stage of the process. shoots ... | 1995 | 24185664 |
| sequence of a novel abscisic acid- and drought-induced cdna from wild tomato (lycopersicon chilense). | | 1993 | 8208856 |
| expression and molecular cloning of drought-induced genes in the wild tomato lycopersicon chilense. | protein synthesis and translatable mrna population changes induced during water stress were studied in leaves of a drought-resistant wild relative of tomato, lycopersicon chilense, using one- and two-dimensional polyacrylamide gel electrophoresis. under our experimental conditions, water deficit did not significantly affect total protein synthesis capacity. however, it induced biphasic synthesis of a new set of proteins. these newly synthesized proteins resumed to control levels upon rehydration ... | 2013 | 1387536 |
| nucleotide diversity patterns of local adaptation at drought-related candidate genes in wild tomatoes. | we surveyed nucleotide diversity at two candidate genes lenced1 and plc30-15, involved in an aba (abscisic acid) signalling pathway, in two closely related tomato species solanum peruvianum and solanum chilense. our six population samples (three for each species) cover a range of mesic to very dry habitats. the aba pathway plays an important role in the plants' response to drought stress. lenced1 is an upstream gene involved in aba biosynthesis, and plc30-15 is a dehydrin gene positioned downstr ... | 2010 | 20831645 |
| genetic diversity and population structure in the tomato-like nightshades solanum lycopersicoides and s. sitiens. | two closely related, wild tomato-like nightshade species, solanum lycopersicoides and solanum sitiens, inhabit a small area within the atacama desert region of peru and chile. each species possesses unique traits, including abiotic and biotic stress tolerances, and can be hybridized with cultivated tomato. conservation and utilization of these tomato relatives would benefit from an understanding of genetic diversity and relationships within and between populations. | 2010 | 20154348 |
| dissection of the style's response to pollination using transcriptome profiling in self-compatible (solanum pimpinellifolium) and self-incompatible (solanum chilense) tomato species. | tomato (solanum lycopersicum) self-compatibility (sc) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. self-incompatibility (si) is defined as self-pollen tubes that are prevented from developing in the style. to determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of sc and si tomato unpollinated/pollinated styles was performed using rna-sequencing (rna-seq) data. | 2015 | 25976872 |
| molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (lycopersicon esculentum) and lycopersicon chilense. | somatic hybrid plants were recovered following fusion of leaf mesophyll protoplasts isolated from tomato (lycopersicon esculentum) cultivar uc82 with protoplasts isolated from suspension cultured cells of l. chilense, la 1959. iodoacetate was used to select against the growth of unfused tomato protoplasts. two somatic hybrids were recovered in a population of 16 regenerants. no tomato regenerants were recovered; all of the non-hybrid regenerants were l. chilense. the l. chilense protoplast regen ... | 1992 | 24212877 |
| bayesian variable selection for detecting adaptive genomic differences among populations. | we extend an f(st)-based bayesian hierarchical model, implemented via markov chain monte carlo, for the detection of loci that might be subject to positive selection. this model divides the f(st)-influencing factors into locus-specific effects, population-specific effects, and effects that are specific for the locus in combination with the population. we introduce a bayesian auxiliary variable for each locus effect to automatically select nonneutral locus effects. as a by-product, the efficiency ... | 2008 | 18245358 |