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isolation and fusion studies on protoplasts from pollen tetrads.different enzymes were tested for isolation of intact protoplasts from pollen tetrads. about 80% isolation was achieved from pollen tetrads of cajanus cajan and zea mays and about 60% from luffa cylindrica and lycopersicon esculentum after 4 h of treatment with 5% cellulase. when these mononucleate protoplasts were incubated in presence of 0.05 m cacl2 in 0.3 m glucose at ph 10.5, 70-80% fusion was achieved. fusion was rare in sodium nitrate solutions.1977321241
the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...1979471028
correlation of genetic and physical structure in the region surrounding the i2 fusarium oxysporum resistance locus in tomato.the dominant gene i2 confers on tomato (lycopersicon esculentum) resistance against the fungus fusarium oxysporum f. sp. lycopersici race 2. a restriction fragment length polymorphism (rflp) marker, tg105, has recently been found to be tightly linked to i2. the potential for cloning this gene by a reverse genetics approach prompted us to describe in both genetic and physical detail the region surrounding the i2 locus on chromosome 11. we have analyzed patterns of segregation of rflp markers on c ...19921346546
a comparison of the polypeptide isoelectric points and antigenic determinant sites of the large subunit of fraction 1 protein from lycopersicon esculentum, nicotiana tabacum and petunia hybrida.the large subunit of fraction 1 protein from lycopersicon esculentum, nicotiana tabacum and petunia hybrida has been examined by isoelectric focusing of the s-carboxymethylated polypeptides, and by double immunodiffusion with antiserum raised against fraction 1 protein. the immunological results reveal heterogeneity in the large subunit primary structure not identified by isoelectric focusing. a variable phylogeny can be generated depending on whether serological or electrofocusing criteria are ...197877685
alterations in cell-surface carbohydrates of rat large granular lymphocytes associated with interleukin-2 activation.the activation of large granular lymphocytes (lgls)/natural killer (nk) cells with interleukin-2 (il-2) has been shown to increase the ability of these cells to lyse nk-resistant tumor target cells. activated lgls, termed lak (lymphokine-activated killer) cells, have been demonstrated to be of therapeutic value in vivo against metastatic tumors. the mechanism by which il-2 induces broadened cytolytic capability, as well as the molecular basis of target recognition and killing by the activated ce ...19902328530
differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by lycopersicon esculentum and griffonia simplicifolia i lectins.in the rat lung, we found that the lycopersicon esculentum (lea) lectin specifically binds to the epithelium of bronchioles and alveoli whereas griffonia simplicifolia i (gs-i) binds to the endothelium of alveolar capillaries. the differential binding affinity of these lectins was examined on semithin (approximately 0.5 microns) and thin (less than 0.1 (microns) frozen sections of rat lung lavaged to remove alveolar macrophages. on semithin frozen sections, lea bound to epithelial cells lining b ...19911879433
survey of plastid rna abundance during tomato fruit ripening: the amounts of rna from the orf 2280 region increase in chromoplasts.a comprehensive survey of the levels of plastid rnas at progressive stages of tomato fruit ripening was conducted by hybridizing total rna with labeled pst i fragments that cover almost the entire tomato plastid genome and with gene-specific probes. two different cultivars of tomato (lycopersicon esculentum mill.) were examined, traveler 76 and count ii. one of the tomato probes, p7, revealed a pronounced increase in the amount of an 8.3 kb rna in ripe fruit. the homologous region of the tobacco ...19911718481
lectin histochemical characteristics of the canine female mammary gland.twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ...19901700652
bioadhesion by means of specific binding of tomato lectin.the possibility of developing bioadhesive drug delivery systems on the basis of molecules which selectively bind to the small intestinal epithelium by specific, receptor-mediated mechanisms was investigated using a lectin isolated from tomato fruits (lycopersicum esculentum). the tomato lectin (tl) was found to bind specifically onto both isolated, fixed pig enterocytes and monolayers of human caco-2 cell cultures with a similar affinity. tl-coated polystyrene microspheres (0.98 micron) also sho ...19921495901
carbohydrate composition of human megakaryocyte membranes in culture: identification using binding of seven lectins.seven tetramethylrhodamine b isothiocyanate- (tritc) labeled lectins: lens culinaris (lch), ulex europeus-1 (uea-1), lycopersicon esculentum (lea), wheat germ agglutinin (wga), dolichos biflorus (dba), soybean agglutinin (sba) and erythrina cristagalli (eca) were applied on cultured human megakaryocytes (megs) detected by immunofluorescence. all stages of megs (from lymphocyte-like megs to mature megs) and platelets were labeled by lch, lea, uea-1 and wga. eca binds to platelets but only to some ...19912061623
indian bunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid.a viroid has been isolated from tomato plants affected by indian bunchy top disease of tomato (lycopersicon esculentum mill.). in dot blot hybridization assays with 32p-labelled crna probes specific for the detection of various viroids, the indian viroid was shown to be most closely related to the citrus exocortis viroid (cevd). sequence determination showed that the viroid consists of 372 nucleotides and confirmed its close relationship with cevd. the viroid, for which we propose the acronym ce ...19911875190
an alignment of 17 deduced protein sequences from plant, fungi, and ciliate h(+)-atpase genes.seventeen protein sequences of h(+)-atpases from plants (arabidopsis thaliana, nicotiana plumbaginifolia, lycopersicum esculentum), fungi (saccharomyces cerevisiae, schizosaccharomyces pombe, zygosaccharomyces rouxii, neuropora crassa, candida albicans), and a parasitic ciliate (leishmania donovani) have been aligned. twenty sequence fragments were identified which were conserved in h(+)-, na+/k(+)-, and ca++ plasma membrane-atpases. in addition, a total of 118 residues not located in these frag ...19921328178
use of lectins for characterization and purification of human bone marrow cells that express cd34.the binding of lectins to nucleated cells from human bone marrow was studied in a search for markers that can be used to subdivide further immature hemopoietic cells that are characterized by their expression of cd34. low-density bone marrow cells were indirectly labeled with biotinylated lectins and streptavidin-r-phycoerythrin (sa-rpe) together with fitc-labeled monoclonal anti-cd34. four-parameter flow cytometric analysis was then performed and list mode data analyzed. of the 21 lectins teste ...19921285403
soluble proteins and hydrolases during crown-gall induction in the tomato, lycopersicon esculentum.soluble proteins isolated from tissues of the tomato lycopersicon esculentum, after inoculation with agrobacterium tumefaciens to induce tumours, have been examined by gel electrophoresis and cytochemically. changes that occur include the suppression of host enzymes, the appearance of bacterial enzymes in the host tissues and the appearance of new enzyme bands in the affected cells. these changes are detectable within 6 hr of infection and prior to evident morphological changes, and may be expl ...1976178634
transcriptional regulation and dna methylation in plastids during transitional conversion of chloroplasts to chromoplasts.during transitional conversion of chloroplasts to chromoplasts in ripening tomato (lycopersicon esculentum) fruits, transcripts for several plastid genes for photosynthesis decreased to undetectable levels. run-on transcription of plastids indicated that transcriptional regulation operated as a predominant factor. we found that most of the genes in chloroplasts were actively transcribed in vitro by escherichia coli and soluble plastid rna polymerases, but some genes in chromoplasts seemed to be ...19902303026
[the detection of different densities of dna in "solanum lycopersicum esc." after absorption of bacteria or bacterial dna]. 19674166473
time-course of phenotypic expression of escherichia coli gene z following transgenosis in haploid lycopersicon esculentum cells. 19734516147
toxin production by alternaria alternata in tomatoes and apples stored under various conditions and quantitation of the toxins by high-performance liquid chromatography.alternaria alternata strain 8442-3 was inoculated into tomatoes (lycopersicon esculentum mill.) and red delicious cultivar apples (malus domestica borkh.). half of the lots of each fruit were shrink-wrapped in high-density polyethylene film. wrapped and unwrapped fruits were incubated under darkness at 4, 15 and 25 degrees c for up to 5 weeks. a high-performance liquid chromatography method was developed to quantitate tenuazonic acid (tea), alternariol (aoh), alternariol methyl ether (ame), and ...19902282281
carbohydrate binding specificities of several poly-n-acetyllactosamine-binding lectins.the structural requirements for the interaction of the asn-linked poly-n-acetyllactosamine-type oligosaccharide moieties of glycoproteins with various n-acetylglucosamine-binding lectins were investigated by means of affinity chromatography on immobilized lectin-sepharose columns. high molecular weight glycopeptides containing poly-n-acetyllactosamine-type oligosaccharides obtained by pronase digestion of human erythrocyte ghosts were treated with 0.1 m trifluoroacetic acid at 100 degrees c for ...19902152329
trypanosomatid protozoa in fruit of solanaceae in southeastern brazil.fruits of cultivated and indigenous solanaceae from southeastern brazil have been examined for the presence of trypanosomatid flagellates. the 14 species found infected were: capsicum annuum, c. praetermissum, lycopersicon esculentum, nicandra physaloides, physalis angulata, solanum sp., s. americanum, s. concinnum, s. diflorum, s. erianthum, s. gilo, s. robustum, s. variable and s. viarum. the pentatomid hemipteran arvelius albopunctatus experimentally transmitted flagellates to fruits of some ...19902152193
translocation of dna of bacterial origin in lycopersicum esculentum by ultracentrifugation in caesium chloride gradient. 19674964238
expression and sequence analysis of cdnas induced during the early stages of tuberisation in different organs of the potato plant (solanum tuberosum l.).cdna clones of two genes (tub8 and tub13) which show a 25-30-fold increase in transcript in the stolon tip during the early stages of tuberisation, have been isolated by differential screening. these genes are also expressed in leaves, stems and roots and the expression pattern in these organs changes on tuberisation. southern analysis shows homologous sequences in the non-tuberising wild type potato species solanum brevidens and in lycopersicon esculentum (tomato). sequence analysis reveals a h ...19921450379
alternative histochemical markers for skeletal muscle capillaries: a statistical comparison among three muscles.except in thin muscles, the analysis of muscle capillary depends on direct ultrastructural visualization or histochemical identification of the capillaries using stains for basement membrane or reactions for endothelial cell enzymes. the accuracy of existing histochemical methods for detecting capillaries has not been systematically tested, however. the purpose of the present study was to compare muscle capillarity determined by two methods currently in use, griffonia simplicifolia i lectin (gsi ...19921386405
ascorbate peroxidase activity in resistant and susceptible plants of lycopersicon esculentum.activity of redox-enzymes of aa system and of catalase was measured in two near-isogenic tomato lines, respectively resistant and susceptible to tobacco mosaic virus infection. afr reductase, dha reductase and catalase showed quite similar activities in both lines, whereas aa peroxidase activity in resistant plants was 75% higher than in susceptible ones, with km values about 4-fold lower. these data suggest that hydrogen peroxide scavenging operated by aa peroxidase could play an important role ...19921295516
degradation of tomato pathogenesis-related proteins by an endogenous 37-kda aspartyl endoproteinase.as a response to the stress induced by different afflicting agents, tomato plants (lycopersicon esculentum) produce the so-called 'pathogenesis-related' proteins. here we report the degradation of some of these proteins by a constitutive endoproteolytic activity that co-distributes with pathogenesis-related proteins in the intercellular spaces of tomato leaves infected with citrus exocortis viroid. this endoproteinase was purified, showing a ph optimum of 2.5-3.5, a mr of 37,000 and selective in ...19892680484
poly-n-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. identification of a tomato-lectin binding 60-90 x 10(3) mr membrane glycoprotein of tubulovesicles.the cytoplasmic tubulovesicular and canalicular membranes of gastric parietal cells are intimately involved in hydrochloric acid secretion. to characterise the glycoproteins of these membranes, we examined a panel of lectins for reactivity with parietal cells in paraffin sections of rat, dog and pig stomach. the poly-n-acetyllactosamine-specific lectin from lycopersicon esculentum (tomato) and from solanum tuberosum (potato), and the galactose-specific lectin ricinus communis agglutinin (rca120) ...19902166741
datura lectin is both an anti-mitogen and a co-mitogen acting synergistically with phorbol ester.the lectins from datura stramonium, lycopersicon esculentum, and solanum tuberosum are structurally related and possess a similar carbohydrate specificity, yet the datura lectin is mitogenic for human lymphocytes while the other two are not. however, the datura lectin was found to antagonize blast transformation induced by purified protein derivative (ppd), even within the concentration range at which it was optimally mitogenic on its own. the presence of a submitogenic concentration of the phor ...19883124260
conserved upstream sequence elements in plant 5s ribosomal rna-encoding genes.as a basis for further comparative studies, nuclear 5s rrna gene repeats from two plants of the solanaceae family, tobacco (nicotiana rustica) and tomato (lycopersicon esculentum), were isolated and sequenced. the more abundant 5s rrna gene repeat in tobacco is 430 bp long, while a second less common variant is 521 bp long. in contrast, the 5s rrna gene repeat from tomato is only 355 bp long. the spacer sequences from these gene repeats, as well as from other published plant nuclear 5s rrna gene ...19911937022
interactions between citrus exocortis and potato spindle tuber viroids in plants of gynura aurantiaca and lycopersicon esculentum.plants of gynura aurantiaca and tomato (lycopersicon esculentum) were inoculated with severe strains of citrus exocortis viroid (cev) and potato spindle tuber viroid (pstv). the progeny of both viroids was analyzed by two systems of page which allowed discrimination between cev and pstv on the basis of their different sizes. when inoculated separately to g. aurantiaca cev and pstv induced severe and very mild symptoms, respectively, with cev accumulating to a higher level than pstv, whereas when ...19892722468
host susceptibility of the papaya mosaic virus in sri lanka.75 plant species from 11 families were tested in sri lanka for their susceptibility to transferring the papaya mosaic virus. after inoculation with this virus, six species, cucurbita pepo, cucumis sativus, nicotiana tabacum, chenopodium amaranticolor, gomphrena globosa and lycopersicum esculentum, developed such symptoms, and after re-isolation from the host plant the virus again infected papaya plants. thus these species are possible alternate hosts of papaya mosaic virus in sri lanka.19817342959
identification of group b streptococcal antigen with lectin-bound polystyrene particles.the lectin of the tomato, lycopersicon esculentum, or of the potato, solanum tuberosum, can be passively coupled to amide-modified polystyrene spheres to be used as a detection reagent for the specific identification of group b streptococcal cultures grown in selective or nonselective todd-hewitt broth for 5 and 4 h, respectively. agglutination occurred when the lectin reagents were allowed to react with either the cell suspension, clarified broth, or antigen extracts from group b streptococci g ...19873301888
different blue-light requirement for the accumulation of transcripts from nuclear genes for thylakoid proteins in nicotiana tabacum and lycopersicon esculentum.we have isolated recombinant lambda gt11 phages which carry cdna clones for the major light-harvesting chlorophyll a/b-binding proteins of photosystem i (lhcpi) and ii (lhcpii), subunit ii of photosystem i, a chlorophyll a/b-binding protein of photosystem ii (cp24), the rieske iron-sulphur protein of the cytochrome b6/f complex, and the 33, 23 and 16 kda proteins of the water-oxidizing complex of photosystem ii from nicotiana tabacum. the nucleotide sequences of cdna clones encoding the precurso ...19911770404
ethylene-regulated expression of a tomato fruit ripening gene encoding a proteinase inhibitor i with a glutamic residue at the reactive site.we report the isolation from tomato (lycopersicon esculentum) of an ethylene-responsive member of the proteinase inhibitor gene family. dna sequence analysis of a full-length cdna clone indicates that the ethylene-responsive gene is distantly related to the tomato proteinase inhibitor i gene, having 53% sequence identity. the predicted amino acid sequence reveals 47% and 45% sequence identity with the tomato and potato proteinase inhibitor i polypeptides, respectively. additionally, the ethylene ...19882903499
inhibition of human lymphocyte transformation by tomato lectin.the lectin from tomato (lycopersicon esculentum) fruits was found to be non-mitogenic for human lymphocytes in culture and actually suppressed spontaneous dna synthesis. it also inhibited the transformation of human peripheral blood lymphocytes induced by recall antigens or allogeneic cells in vitro. this inhibition was most effective when the lectin was present from the beginning of the culture period, and could be abolished by the simultaneous addition of oligomers of n-acetylglucosamine. the ...19862941859
synthetic antimicrobial peptide design.to guide the design of potential plant pathogen-resistance genes, synthetic variants of naturally occurring antimicrobial gene products were evaluated. five 20-amino acid (esf1, esf4, esf5, esf6, esf13), one 18-amino acid (esf12), and one 17-amino acid (esf17) amphipathic peptide sequences were designed, synthesized, and tested with in vitro bioassays. positive charges on the hydrophilic side of the peptide were shown to be essential for antifungal activity, yet the number of positive charges co ...19957579625
the activation process of arabidopsis thaliana a1 gene encoding the translation elongation factor ef-1 alpha is conserved among angiosperms.in arabidopsis thaliana, the activation process of the a1 ef-1 alpha gene depends on several elements. using the gus reporter gene, transient expression experiments have shown that mutations of upstream cis-acting elements of the a1 promoter, or the deletion of an intron located within the 5' non-coding region, similarly affect expression in dicot or monocot protoplasts. the results reported here strongly suggest that this 5' intron is properly spliced in zea mays. we show that two trans-acting ...19921600144
functional heterogeneity between nkr-p1bright/lycopersicon esculentum lectin (l.e.)bright and nkr-p1bright/l.e.dim subpopulations of rat natural killer cells.in this report, we present data on heterogeneity of rat nk cells utilizing a combination of antibody and lectin-binding characteristics. among nkr-p1bright nk cells, two discrete populations characterized as lycopersicon esculentum lectin (l.e.)bright (60 to 80%) and l.e.dim (20 to 40%) were identified by flow cytometry. comparison of the morphology of sorted nkr-p1bright/l.e.bright and nkr-p1bright/l.e.dim cells indicated that both were greater than 90% lgl. an analysis of the functional capabi ...19921588052
a tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway.in the initial stages of carotenoid biosynthesis in plants the enzyme phytoene synthase converts two molecules of geranylgeranyl diphosphate into phytoene, the first carotenoid of the pathway. we show here that a tomato (lycopersicon esculentum) cdna for a gene (psy1) expressed during fruit ripening directs the in vitro synthesis of a 47-kda protein which, upon import into isolated chloroplasts, is processed to a mature 42-kda form. the imported protein is largely associated with membranes, but ...19921544888
evidence for selection as a mechanism in the concerted evolution of lycopersicon esculentum (tomato) genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.the nuclear gene sequences encoding rbcs, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (ec 4.1.1.39) from several plants show extensive interspecific divergence but little intraspecific divergence, suggesting that these genes are evolving in concert within a genome. in this study, the nucleotide sequences of two tomato (lycopersicon esculentum) rbcs genes and a cdna clone containing the entire coding region of a third tomato rbcs gene were determined. the three genes, des ...19863012537
nucleotide sequence and molecular evolution of two tomato genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase.we have isolated and sequenced two cdna clones (less5 and less17) encoding the small subunit of ribulose-1,5-bisphosphate carboxylase of tomato (lycopersicon esculentum). at the nucleotide level, the protein-coding regions of these genes are 85% conserved, while the untranslated 3' regions are only 55% conserved. comparison with rbcs genes from other species of solanaceae suggests that the tomato less5 gene, the nicotiana tabacum ntss23 gene and the petunia hybrida ssu8 gene are orthologous memb ...19863557127
legume agglutinins that bind to rhizobium meliloti.a protein found in seeds and roots of alfalfa (medicago sativa) was implicated in the specificity of the infection process, based on its binding to the symbiont rhizobium meliloti. we found an agglutinin with similar properties in seeds and roots of sweet clover (melilotis alba). the sweet clover differed from alfalfa in nodulation by a mutant strain of r. meliloti, but the agglutinins were indistinguishable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rhizobium agglutination, a ...19853988714
differential binding of the lectins griffonia simplicifolia i and lycopersicon esculentum to microvascular endothelium: organ-specific localization and partial glycoprotein characterization.the lectins griffonia simplicifolia i and lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and thin (less than 0.1 micron) frozen sections that were reacted with biotinylated lectin followed by streptavidin conjugated to texas red, for semithin sections, or by streptavidin con ...19902328740
transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus.the tomato yellow leaf curl virus (tylcv) gene that encodes the capsid protein (v1) was placed under transcriptional control of the cauliflower mosaic virus 35s promoter and cloned into an agrobacterium ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, lycopersicon esculentum x l. pennellii (f1), sensitive to the tylcv disease. when transgenic f1 plants, expressing the v1 gene, were inoculated with tylcv using whiteflies fed on tylcv-infected plants, they respo ...19947764709
a comparison of tomato (lycopersicon esculentum) lectin with its deglycosylated derivative.a deglycosylated derivative of tomato (lycopersicon esculentum) lectin was prepared with the use of trifluoromethanesulphonic acid. its properties were generally similar to those of the native lectin, but differences were evident in terms of relative agglutinating activity towards sheep, (untreated) human and trypsin-treated human erythrocytes. the two forms of tomato lectin were used in conjunction with a battery of specific antisera to investigate structural relatedness among solanaceous lecti ...19846087799
effects of light on the translocation of bacterial dna in solanum lycopersicum esc. 19674864818
effect of temperature on the translocation of bacterial dna in solanum lycopersicum esc. 19684880493
tomato (lycopersicon esculentum) lectin and serologically related molecules. 19836201869
[microorganisms of the mycoplasma type in the phloem cells of solanum lycopersicum l. with stolbur]. 19684971201
heterochromatin, the synaptonemal complex and crossing over.a combined light- and electron-microscopic examination of chromosomes from two angiospermous plants, plantago ovata and lycopersicon esculentum, and a mammal, mus musculus, was performed. from this investigation three observations have been made that may be relevant to the observed lack of crossing over in heterochromatin. (1) differential staining indicates that heterochromatin represents a smaller fraction of the length of pachytene chromosomes than it represents in the length of mitotic metap ...19846520145
isolation, in vitro culture, ultrastructure study, and characterization by lectin-agglutination tests of phytomonas isolated from tomatoes (lycopersicon esculentum) and cherimoyas (anona cherimolia) in southeastern spain.plants of lycopersicon esculentum (grown in greenhouses) and anona cherimolia cultivated in southeastern spain were examined for the presence of trypanosomatid flagellates. kinetoplastid protozoa were found in the fruits but not in the phloem or other plant tissues. parasites were detected from the onset of fruiting. isolates were detected from the onset of fruiting. isolates were adapted to in vitro culturing in monophase media. the form and the structural organization was studied by scanning a ...19957479649
cytokeratin peptide sfgsgfgggy mimics n-acetyl-beta-d-glucosamine in reaction with antibodies and lectins, and induces in vivo anti-carbohydrate antibody response.recently, we found that human anti-glcnac mabs reacted with keratin from human skin and recognized specific peptide epitopes of human cytokeratin 14. our data demonstrate that anti-keratin ab responses in mice might be driven by ags bearing terminal o-linked glcnac residues. to determine if mouse anti-glcnac mabs recognized peptide epitopes of keratin, several mouse anti-glcnac mabs were reacted with a panel of overlapping synthetic decapeptides of the entire amino acid sequence of human cytoker ...19947527445
characterization of three agrobacterium tumefaciens avirulent mutants with chromosomal mutations that affect induction of vir genes.three agrobacterium tumefaciens mutants with chromosomal mutations that affect bacterial virulence were isolated by transposon mutagenesis. two of the mutants were avirulent on all hosts tested. the third mutant, ivr-211, was a host range mutant which was avirulent on bryophyllum diagremontiana, nicotiana tabacum, n. debneyi, n. glauca, and daucus carota but was virulent on zinnia elegans and lycopersicon esculentum (tomato). that the mutant phenotype was due to the transposon insertion was dete ...19911846853
ovarian angiogenesis. phenotypic characterization of endothelial cells in a physiological model of blood vessel growth and regression.angiogenesis occurs during embryogenesis and is a down-regulated process in the healthy adult that is almost exclusively linked to pathological conditions such as tumor growth, wound healing, and inflammation. physiological angiogenic processes in the adult are restricted to the female reproductive system where they occur cyclically during the ovarian and uterine cycle as well as during pregnancy. by systematically analyzing the phenotypic changes of endothelial cells during bovine corpus luteum ...19957543733
the characterisation of tapetum-specific cdnas isolated from a lilium henryi l. meiocyte subtractive cdna library.differential screening of a meiocyte subtractive cdna library from lilium henryi l. has identified a group of 16 anther-specific partial cdnas. three of these sequences, lhm2, lhm6 and lhm7 have been further characterised. hybridisation in situ with antisense riboprobes of lhm2, lhm6, and lhm7 gives a strong, clear signal which, contrary to expectations, is localised to the tapetal layer surrounding the meiocytes and not the meiocytes themselves. developmental slot blots demonstrate that mrnas c ...19957647684
a view of plant dehydrins using antibodies specific to the carboxy terminal peptide.dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ...19937693020
confocal laser scanning microscopy of mitochondria within microspore tetrads of plants using rhodamine 123 as a fluorescent vital stain.the present study demonstrates that rhodamine 123 penetrates the callose walls surrounding plant microspores before they are released from tetrads. the stain accumulates in active mitochondria due to the electrical potential across the mitochondrial membrane. accumulation of dye does not occur in mitochondria of fixed cells and fades quickly when mitochondrial activity is inhibited by exposure to carbonyl cyanide m-chlorophenyl hydrazone. rhodamine can be used as a viability test for microspores ...19947703302
characterization of glycoconjugate expression during development of meckel's cartilage in the rat.the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ...19957717533
expression of membrane glycoconjugates on sheep lung endothelium.carbohydrates play an important role in both the regulation and expression of endothelial cell surface molecules.19957723284
effect of temperature on cucumber mosaic virus satellite-induced lethal tomato necrosis is helper virus strain dependent.the effect of temperature on the response of tomato (lycopersicon esculentum mill. cv. rutgers) to infections with the necrogenic cucumber mosaic virus (cmv) satellite d-carna 5 was investigated with each of four cmv strains d, 1, y and s functioning as helper virus. at 24 degrees c lethal necrosis was observed in all infections. however, at 32 degrees c the response varied from total absence or reduction of necrosis with some strains to accelerated lethal necrosis with others. the total lack of ...19957544110
genetic control of plastid differentiation. 3 ultrastructure of plastids in different green revertant spots of the plastom mutant pl-alb1 of lycopersicon esculentum.reverse mutations in plastid dna give rise to phenotypically different green spots on the white tissues of the homoplastic plastom mutant pl-alb1. the structure of the plastids in one yellow-green, one green and one dark-green spot, each arising by independent reverse mutation, was studied by means of electron microscopy. although the same revertant plastid types (type 1 with a weakly developed lamellar system; type 2 with vacuolized grana, and type 3 with a wild type-like structure were found i ...19817238120
effect of sodium citrate on the translocation of bacterial dna in solanum lycopersicum esc. 19684884950
an ultrastructural study of the mature chromoplasts of the tangerine tomato (lycopersicon esculentum var. "golden jubilee"). 19676080099
high-resolution mapping of the physical location of the tomato cf-2 gene.to isolate the tomato cf-2 resistance gene by map-based cloning, plants recombinant for rflp markers close to cf-2 were selected by exploiting the flanking morphological markers yv (yellow virescent) and tl (thiaminless). using these recombinants, a high-resolution linkage map of the region encompassing the cf-2 gene has been generated containing several new rflp markers. mapping of two yac clones carrying lycopersicon esculentum and l. peruvianum dna, indicates that in both genotypes the physic ...19957756690
immunocytolocalization of 1-aminocyclopropane-1-carboxylic acid oxidase in tomato and apple fruit.the subcellular localization of 1-aminocyclopropane-1-carboxylic acid oxidase (acc oxidase), an enzyme involved in the biosynthesis of ethylene, has been studied in ripening fruits of tomato (lycopersicum esculentum mill.). two types of antibody have been raised against (i) a synthetic peptide derived from the reconstructed ptom13 clone (prc13), a tomato cdna encoding acc oxidase, and considered as a suitable epitope by secondary-structure predictions; and (ii) a fusion protein overproduced in e ...19947764617
a cytochemical study of acid phosphatases and esterases during the induction of crown gall in the tomato. lycopersicon esculentum. 19715099263
pathogenesis-related pr-1 proteins are antifungal. isolation and characterization of three 14-kilodalton proteins of tomato and of a basic pr-1 of tobacco with inhibitory activity against phytophthora infestans.three distinct basic 14-kd proteins, p14a, p14b, and p14c, were isolated from tomato (lycopersicon esculentum mill. cv baby) leaves infected with phytophthora infestans. they exhibited antifungal activity against p. infestans both in vitro (inhibition of zoospore germination) and in vivo with a tomato leaf disc assay (decrease in infected leaf surface). serological cross-reactions and amino acid sequence comparisons showed that the three proteins are members of the pr-1 group of pathogenesis-rel ...19957784503
differential lectin binding to the fibrinoid of human full-term placenta: correlation with a fibrin antibody and the paf-halmi method.placental fibrinoid is thought to contain various glycoproteins originating from cell secretion and tissue degeneration, occasionally merged with fibrin. information on the characteristics and derivation of the various fibrinoid components, however, is still fragmentary. therefore, the present histochemical study on acetone-fixed placental tissue sections compared the staining pattern of fitc-conjugated lectins from ulex europaeus (uea-i), bandeiraea simplicifolia (bs-i) and lycopersicon esculen ...19947817713
analysis of nuclear and organellar dna of somatic hybrid calli and plants between lycopersicon spp. and nicotiana spp.protoplast fusion experiments between lycopersicon esculentum or l. peruvianum and nicotiana tabacum or n. plumbaginifolia were performed to investigate the possibility of producing symmetric and asymmetric somatic hybrids between these genera. these fusions, which involved 1.7 x 10(8) protoplasts, yielded 35 viable hybrid calli. plant regeneration was successful with two calli. one of these regenerants flowered, but developed no fruits. analysis of the nuclear dna by means of dot blot hybridiza ...19937903413
overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis.tomato plants (lycopersicon esculentum, var. better boy) were stably transformed with a gene consisting of the open reading frame of a prosystemin cdna under the regulation of the cauliflower mosaic virus 35s promoter. the leaves of the transgenic plants constitutively produced proteinase inhibitor i and ii proteins, which accumulated over time to levels exceeding 1 mg/g of dry leaf weight. this phenotype contrasts with that of untransformed plants, which produce proteinase inhibitor proteins in ...19947937894
orf c4 of tomato leaf curl geminivirus is a determinant of symptom severity.a tomato leaf curl virus (tlcv) mutant has been constructed in vitro that contains t-to-c replacements at nucleotides 2457 and 2463 within the c4 open reading frame (orf). the mutations destroy the two possible initiator aug codons for the c4 orf without disrupting the coding capacity of the c1 orf which entirely overlaps the c4 orf. agroinoculation of the c4 mutant tlcv into three alternative experimental hosts for the virus (datura stramonium, lycopersicon esculentum, and nicotiana tabacum) gi ...19947941358
histochemical study by lectin binding of surface glycoconjugates in the developing olfactory system of rat.lectin-binding histochemistry was used to investigate the distribution and density of defined carbohydrate sequences on the cell surface glycoproteins of the olfactory receptors of rat during development. the olfactory and vomeronasal receptors showed a positive labelling after biotinylated lycopersicum esculentum lectin binding on embryonic day 16 (e16), while horseradish peroxidase-labelled glycine max, bandeiraea simplicifolia (bsa-i) and its b4 isomer bsa-i-b4 agglutinins started to label fr ...19947942093
a self-stabilizing ac derivative and its potential for transposon tagging.with the goal of developing a self-stabilizing transposon tagging system, a derivative of the maize transposable element ac (ds303) harbouring a deletion between bp 246 and 736 has been introduced into tomato (lycopersicon esculentum) by agrobacterium tumefaciens-mediated transformation. the deletion removes the major transcription start site, 84 bp of the putative ac promoter and part of the untranslated leader. transpositions from the t-dna, where ds303 was inserted between the mannopine synth ...19948000429
a study on the local anaesthetic activity of the acetone extract of lycopersicon esculentum leaves. 19695308410
demonstration of poly-n-acetyl lactosamine residues in ameboid and ramified microglial cells in rat brain by tomato lectin binding.this study was designed to demonstrate the localization of poly-n-acetyl lactosamine residues in postnatal and adult rat brain, visualized by their specific binding to a lectin obtained from lycopersicon esculentum (tomato). lectin histochemistry was carried out on cryostat, paraffin, and vibratome sections and was examined by light microscopy. selected vibratome sections were processed for electron microscopy. our results showed that tomato lectin histochemistry was found in relation to blood v ...19948027523
transgenic tomato lines containing ds elements at defined genomic positions as tools for targeted transposon tagging.we have introduced a genetically marked dissociation transposable element (dshpt) into tomato (lycopersicon esculentum) by agrobacterium tumefaciens-mediated transformation. probes for the flanking regions of the t-dna and transposed dshpt elements were obtained with the inverse polymerase chain reaction (ipcr) technique and used in rflp linkage analyses. the rflp map location of 11 t-dnas carrying dshpt was determined. the t-dnas are distributed on 7 of the 12 tomato chromosomes. to explore the ...19948028583
isolation of a mrna encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding.a cdna clone (tab2) encoding a nucleoside diphosphate (ndp) kinase has been isolated from a tomato (lycopersicon esculentum mill. cv. ailsa craig) cdna library. the clone is 590 bp long and exhibits a high degree of sequence identity with spinach ndp kinases i and ii, pisum sativum ndp kinase i, arabidopsis thaliana ndp kinase, drosophila melanogaster ndp kinase, dictyostelium discoideum ndp kinase and human nm 23-h1 and nm23-h2. northern analysis has revealed that the mrna encoded by tab2 is up ...19948061324
isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mrna levels during fruit ripening.to determine the relationship between invertase gene expression and glucose and fructose accumulation in ripening tomato fruit, fruit vacuolar invertase cdna and genomic clones from the cultivated species, lycopersicon esculentum cv. uc82b, and a wild species, lycopersicon pimpinellifolium, were isolated and characterized. the coding sequences of all cdna clones examined are identical. by comparison to the known amino acid sequence of mature l. esculentum fruit vacuolar invertase, a putative sig ...19938095164
on the safe use of pesticides in controlling the terrestrial mollusc pests.the preferred food items of the slugs laevicaulis alte and the snails achatina fulica were used to prepare 'poison baits' by injecting the pesticides 'rogor' and 'nuvan' to kill these mollusc pests. the 'poison baits' prepared with trichosanthes dioica and lycopersicum esculentum were accepted by 100% individuals of both the species irrespective of the pesticides used. in all cases the slug and snail individuals died within a considerable length of time following consumption of the bait. the imp ...19938107590
structure of ten free n-glycans in ripening tomato fruit. arabinose is a constituent of a plant n-glycan.the concentration-dependent stimulatory and inhibitory effect of n-glycans on tomato (lycopersicon esculentum mill.) fruit ripening was recently reported (b. priem and k.c. gross [1992] plant physiol 98: 399-401). we report here the structure of 10 free n-glycans in mature green tomatoes. n-glycans were purified from fruit pericarp by ethanolic extraction, desalting, concanavalin a-sepharose chromatography, and amine-bonded silica high performance liquid chromatography. n-glycan structures were ...19938108510
segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium.the barrier function, surface biochemistry, and morphology of confluent monolayers of endothelial cells isolated from different segments of the bovine lung vasculature [microvessels (blmvec), vein (bpvec) and artery (bpaec)] were grown in culture and compared. a number of common cell surface proteins were identified along with two proteins of 46 and 48 kda found exclusively on bpvec. lectin affinity chromatography revealed multiple glycosylation differences. the lectins, arachis hypogaea (aha) a ...19948123001
a stable 463 nucleotide variant of citrus exocortis viroid produced by terminal repeats.an unusual variant of citrus exocortis viroid (cev) was detected when an inoculum source from gynura aurantiaca d.c. was used to infect a hybrid tomato (lycopersicon esculentum mill. x l. peruvianum). the 92 nucleotide larger variant, cev d-92, which displayed the characteristic circular and linear viroid structural forms, contained two repeated sequences spanning the v and t2 domains. a dramatic moderation of symptom expression in gynura accompanied the incorporation of these repeated sequences ...19948151291
a gene encoding a novel isoform of the pr-1 protein family from tomato is induced upon viroid infection.a lycopersicon esculentum cdna clone encoding an acidic-type pathogenesis-related protein (pr-1a1) was isolated, sequenced and characterized. it contains an open reading frame of 175 amino acids and the mature protein, after cleavage of the 21 amino acid signals peptide, has a pi of 5.24. the protein shows highest homology (75% identity) with the basic pathogenesis-related prb-1b protein from tobacco. the pr-1a1 gene shows constitutive expression in roots from tomato plants. it is expressed in l ...19948190070
structure and expression of lema-1, a tomato protein belonging to the sec18-pas1-cdc48-tbp-1 protein family of putative mg(2+)-dependent atpases.cdna clones of a tomato protein, called lycopersicum esculentum putative mg(2+)-dependent atpase (lema-1), were isolated from a cdna library. sequence comparison of the tomato protein with other genes in the database revealed that the protein is highly homologous to a human protein called tbp-1 and a yeast tat-binding-analogue protein yta1a. all three proteins belong to the recently discovered protein family of putative mg(2+)-dependent atpases and form within this family a subgroup of proteins ...19957766893
cdna cloning of viroid-induced tomato pathogenesis-related protein p23. characterization as a vacuolar antifungal factor.a 23-kd pathogenesis-related protein (p23) is induced in tomato (lycopersicon esculentum mill, cv rutgers) plants when infected with citrus exocortis viroid. this protein is homologous to the salt-induced tomato np24 protein (i. rodrigo, p. vera, r. frank, v. conejero [1991] plant mol biol 16: 931-934). further characterization of p23 has shown that this protein accumulates in vacuoles in association with dense inclusion bodies. in vitro assays indicated that the purified p23 protein inhibits th ...19938278538
molecular heterogeneity of photosystem i. psad, psae, psaf, psah, and psal are all present in isoforms in nicotiana spp.the protein composition of photosystem i (psi) was examined in nicotiana spp. by high-resolution polyacrylamide gel electrophoresis, n-terminal amino acid sequencing, and immunoblot analysis. five psi proteins show polymorphism in an amphidiploid species, nicotiana tabacum, but not in its ancestral diploid species, nicotiana sylvestris and nicotiana tomentosiformis. these nicotiana spp. appear to have at least 18 psi proteins per genome that range in molecular mass from 3 to 20 kd. they include ...19938278548
the persistence of engineered agrobacterium tumefaciens in agroinfected plants.several plant species, including tomato (lycopersicon esculentum), gynura aurantiaca, avocado (persea americana), and grapefruit (citrus paradisi) grafted on troyer citrange (poncirus trifoliata x c. sinensis) were "agro-infected" with agrobacterium tumefaciens strain lba-4404, carrying a mini-ti plasmid with a dimeric cdna of citrus exocortis viroid (cevd). extracts prepared from tissues of the agroinfected plants 38-90 days after inoculation were plated on selective media and found to contain ...19938274776
distribution of saccharides in pig lymph-node high-endothelial venules and associated lymphocytes visualized using fluorescent lectins and confocal microscopy.the distribution of saccharides in pig lymph nodes, particularly on high-endothelial venule (hev) endothelium and on lymphocytes in these vessels, was studied by examining the binding of fluorescent conjugates of 18 different lectins. eight of the lectins, particularly with glycan specificity restricted to mannose and polyacetyllactosamine determinants, were found to bind with a high affinity to these structures. competitive inhibition experiments revealed that polylactosamine-containing glycans ...19938282568
immuno- and lectin histochemistry of epithelial subtypes and their changes in a radiation-induced lung fibrosis model of the mini pig.cell types of lung epithelia of mini pigs have been studied using a panel of monoclonal and polyclonal antibodies against cytokeratins (cks) and vimentin and three lectins before and after radiation-induced fibrosis. in normal tissues, ck18 specific antibodies reacted above all with type ii alveolar epithelial cells, while ck7 and pan ck-specific antibodies stained the whole alveolar epithelium. in bronchial epithelial cells, cks 7, 8, 18 and focally cks 4 and 13 as well as vimentin were found. ...19938307778
lr gold and lr white embedding of lung tissue for immunoelectron microscopy.the immunogold technique in conjunction with lr gold or lr white embedding was used to localize different antigens in normal rat and mini pig lung tissues. lycopersicon esculentum and maclura pomifera lectins and diverse cytoskeletal antigens such as cytokeratins, beta-tubulin, smooth muscle-actin, tropomyosin and villin were labelled well. factor viii-related antigen was found in weibel-palade bodies of endothelial cells. a 10 kda clara cell protein was localized in clara cells. the morphologic ...19938135092
regulation of secretion and surface expression of mac-2, a galactoside-binding protein of macrophages.mac-2, a 30-35-kda galactose-binding protein, is synthesized at similar levels in murine peritoneal exudate macrophages whether recruited in response to an intraperitoneal pathogen mycobacterium microti, to sterile inflammatory stimuli such as thioglycollate broth, or to concanavalin a. in elicited or activated macrophages up to 30% of mac-2 is constitutively secreted, and secretion is stimulated markedly by calcium ionophore a23187. only thioglycollate-elicited macrophages express cell surface ...19948308013
assessment of the mutagenicity of extracts of tmv-coat-protein-gene induced transgenic tomato by the umu-test.we examined the mutagenicity of extracts (juice and ethanol extract) from a transgenic tomato that was established by transfection of a gene encoding the coat protein of tobacco mosaic virus (tmv) to the f1 hybrid between lycopersicon esculentum la1000 and l. peruvianum pi128650, by the umu-test with salmonella typhimurium ta1535/psk1002 as the test organism. the extracts showed no detectable mutagenicity. the extracts from the above-mentioned f1 hybrids and wild tomatoes and cultivars (l. peruv ...19958541659
carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis.an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ...19958550384
replication of tomato yellow leaf curl virus (tylcv) dna in agroinoculated leaf discs from selected tomato genotypes.the leaf disc agroinoculation system was applied to study tomato yellow leaf curl virus (tylcv) replication in explants from susceptible and resistant tomato genotypes. this system was also evaluated as a potential selection tool in breeding programmes for tylcv resistance. leaf discs were incubated with a head-to-tail dimer of the tylcv genome cloned into the ti plasmid of agrobacterium tumefaciens. in leaf discs from susceptible cultivars (lycopersicon esculentum) tylcv single-stranded genomic ...19938400142
leucine aminopeptidase: an inducible component of the defense response in lycopersicon esculentum (tomato).a leucine aminopeptidase (ec 3.4.11.1) cdna clone (dr57) that was induced in response to pseudomonas syringae pv. tomato (p.s. tomato) infection was isolated using a subtractive hybridization-enriched cdna probe. genomic dna blot analysis showed that the tomato genome had two leucine aminopeptidase genes. the levels of dr57 mrnas after p.s. tomato infection and mechanical wounding were determined in two inbred tomato lines that exhibit susceptibility and resistance to p.s. tomato. dr57 mrnas wer ...19938234334
genes encoding acidic and basic class iii beta-1,3-glucanases are expressed in tomato plants upon viroid infection.beta-1,3-glucanases are hydrolytic enzymes considered to constitute part of the general array of defense genes induced by pathogen infection in higher plants. we have isolated and characterized two complementary dna clones, corresponding to new beta-1,3-glucanases from tomato plants (lycopersicon esculentum) which are expressed upon challenge with citrus exocortis viroid. amino acid sequence comparison revealed that they are most similar to beta-1,3-glucanases from tobacco, particularly to pr-q' ...19948193297
isolates of citrus exocortis viroid recovered by host and tissue selection.isolates of citrus exocortis viroid (cev) from a single sweet orange citrus source have been selected by sequential passage through the alternative hosts citron, gynura aurantiaca, a hybrid tomato lycopersicon esculentum x l. peruvianum, and from disorganized callus culture of the hybrid tomato. the distinctions in symptom expression, titre and electrophoretic mobility among the cev isolates, operationally termed cevc (citron), cevg (gynura), cevt (tomato) and cevcls (callus) are supported by ch ...19938245858
le-acs4, a fruit ripening and wound-induced 1-aminocyclopropane-1-carboxylate synthase gene of tomato (lycopersicon esculentum). expression in escherichia coli, structural characterization, expression characteristics, and phylogenetic analysis.acc (1-aminocyclopropane-1-carboxylic acid) synthase is the key regulatory enzyme in the biosynthetic pathway of the plant hormone ethylene and is encoded by a highly divergent multigene family in tomato (rottmann, w. h., peter, g. f., oeller, p. w., keller, j. a., shen, n. f., nagy, b. p., taylor, l. p., campbell, a. d., and theologis, a. (1991) j. mol. biol. 222, 937-961). two members of the family, le-acs2 and le-acs4, are induced during fruit ripening and upon treatment of mature green fruit ...19938366090
localization of binding sites of ulex europaeus i, helix pomatia and griffonia simplicifolia i-b4 lectins and analysis of their backbone structures by several glycosidases and poly-n-acetyllactosamine-specific lectins in human breast carcinomas.several studies have shown the deletion of blood group a or b antigens and the accumulation of h antigens in human breast carcinomas. other studies have independently demonstrated that the binding sites of lectins such as helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin i-b4 (gsai-b4) are highly expressed in these cells. in order to clarify the molecular mechanisms of malignant transformation and metastasis of carcinoma cells, it is important to understand the relationship b ...19968897074
tomato rab1a homologs as molecular tools for studying rab geranylgeranyl transferase in plant cells.rab proteins attach to membranes along the secretory pathway where they contribute to distinct steps in vesicle-mediated transport. to bind membranes, rab proteins in fungal and animal cells must be isoprenylated by the enzyme rab geranylgeranyl transferase (rab ggtase). we have isolated three tomato (lycopersicon esculentum, m.) cdnas (lerab 1a, b, and c) encoding rab-like proteins and show here that all three are substrates for a rab ggtase-like activity in plant cells. the plant enzyme is sim ...19968934628
cdna cloning, expression during development, and genome mapping of psy2, a second tomato gene encoding phytoene synthase.the enzyme phytoene synthase (psy) catalyzes the formation of phytoene, an intermediate in the carotenoid biosynthesis pathway. expression of a previously described gene (psy1) is induced by fruit ripening in tomato (lycopersicon esculentum). we describe here the cloning of a partial cdna for psy2, a gene related to psy1. a plasmid containing the psy2 coding region under control of a bacterial promoter complements a rhodobacter capsulatus phytoene synthase mutant, indicating that this gene has t ...19938245008
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