| [a rna extract from oncogenic and non oncogenic strains of agrobacterium tumefaciens is an indispensable element for the induction of tumors in datura stramomium]. | an rna bound to the reverse transcriptase of agrobacterium tumefaciens has been isolated and shown to be oncogenic for stem tissues of datura stramonium grown under axenic conditions. the tumorous nature of the cellular change induced by the infectious rna was demonstrated by serial grafts of tumors on datura stems and by cultivation of tumorous tissue in vitro on a medium without supplemental auxins and cytokinins. active cellular proliferation within tissues of datura stems was a prerequisite ... | 1976 | 58705 |
| large animal hepatotoxic and nephrotoxic plants. | the hepatotoxic and nephrotoxic plants of large domestic animals have been reviewed. the most important ones are those widely distributed as weeds over pastures, negelcted forests and grasslands, those used as ornamentals, the nitrate concentrating forage crops, and the cyanophoric plants. crotolaria spp, the ragwort (senecia jacobaea), the lantana spp. and heliotopum are common hepatoxic plants. amaranthus retroflexus, datura stramonium, solanum rostratum, and the castor oil plant (ricinus comm ... | 1979 | 516370 |
| studies on lectins. xxxvii. isolation and characterization of the lectin from jimson-weed seeds (datura stramonium l.). | the lectin of jimson-weed seeds (datura stramonium l.) was isolated by affinity chromatography on a polysaccharide mixture from mycelium of aspergillus niger. the lectin yields two bands on disc electrophoresis, it has sedimentation coefficient s20,w = 3.8 s and its apparent molecular weight estimated by thin layer gel chromatography is 120,000. the lectin reduced with mercaptoethanol yields on polyacrylamide gel electrophoresis in the presence of dodecyl sulfate three zones corresponding to sub ... | 1978 | 620059 |
| the biosynthetic route from ornithine to proline. | it is shown by tracer experiments with dl-[2-3h,5-14c]- and dl-[(rs)-5-3h,5-14c]rnithine, that the metabolic conversion of ornithine into proline, in three plant species (nicotiana tabacum, datura stramonium, and lupinus angustifolius), takes place with maintenance of the delta-hydrogen atoms but with loss of the alpha-hydrogen atom. this indicates a route via alpha-keto-delta-aminovaleric acid (5-amino-2-oxopentanoic acid) and disproves the accepted route via glutamic gamma-semialdehyde (2-amin ... | 1979 | 762087 |
| particular small size rna and rna fragments from different origins as tumor inducing agents in datura stramonium. | particular rna fragments obtained by action of pancreatic ribonuclease on purified rnas originating from species totally unrelated to agrobacterium tumefaciens (escherichia coli, rabbit, monkey) are capable of inducing the formation of transplantable tumorous tissue when introduced at wounded sites in inverted stems of datura stramonium maintained under axenic conditions on a medium containing auxin and kinetin. reovirus rna and a small size rna (5-6s) isolated from rna bound rna directed dna po ... | 1976 | 822281 |
| method for the detection of glycopeptides at the picomole level in hplc peptide maps. | glycopeptide-containing fractions in hplc peptide maps can be detected by a simple application of the microtiter plate-bound streptavidin-biotinylated glycopeptide-lectin method (m.-c. shao, 1992, anal. biochem., 205, 77-82). to illustrate this application, the glycoproteins, ovalbumin and asialofetuin, reduced and s-alkylated with vinylpyridine, were digested with trypsin-l-1-p-tosylamino-2-phenylethylchloromethyl ketone and the tryptic peptides were fractionated by reverse-phase hplc, monitori ... | 1992 | 1283290 |
| wga-binding, mucin glycoproteins protect the apical cell surface of mouse uterine epithelial cells. | expression of apical cell surface proteins and glycoproteins was examined in polarized primary cultures of mouse uterine epithelial cells (uec). lectin-gold cytochemistry revealed that wheat germ agglutinin (wga) bound specifically to the components of the apical glycocalyx as well as intracellular vesicles. double labeling with the ph sensitive dye 3-(2,4-dinitroanilino)-3'amino-n-methyldipropylamine (damp) demonstrated the acidic nature of the wga-staining intracellular vesicles. the enzymatic ... | 1992 | 1295897 |
| bandeiraea simplicifolia lectin i and vicia villosa agglutinin bind specifically to the vomeronasal axons in the accessory olfactory bulb of the rat. | the binding of 21 lectins to the accessory olfactory bulb (aob) of the rat was examined by histochemistry. two lectins [bandeiraea simplicifolia lectin i (bsl-i) and vicia villosa agglutinin (vva)] bound specifically to the vomeronasal (vn) axons in the aob. seven lectins (datura stramonium lectin, erythrina cristagalli lectin, lycoperisicon esculentum lectin, ricinus communis agglutinin i, soybean agglutinin, solanum tuberosum lectin, and ulex europaeus agglutinin) bound to both vn axons in aob ... | 1992 | 1314354 |
| storage of glycoprotein in nctr-balb/c mouse. lectin histochemistry, and biochemical studies. | a strain of balb/c mice carrying a lysosomal storage disorder exhibits metabolic and phenotypic abnormalities similar to patients with sphingomyelin-cholesterol lipidoses type ii (i.e., niemann-pick c and d). their foamy cells, which belong to the reticuloendothelial system, stained intensely by periodate-schiff (pas) reagent and were resistant to predigestion with diastase. to identify the chemical nature of the pas-positive storage material, we applied lectin histochemistry and biochemical met ... | 1992 | 1360721 |
| polylactosaminoglycan modification of the respiratory syncytial virus small hydrophobic (sh) protein: a conserved feature among human and bovine respiratory syncytial viruses. | we investigated the nature of the oligosaccharide modification of the glycosylated forms of the small hydrophobic integral membrane protein, sh (previously designated 1a), of respiratory syncytial (rs) virus. analysis of sh protein expressed in cells infected with rs virus or with a recombinant vaccinia virus revealed two glycosylated sh protein species, shg and shp, which contained n-linked carbohydrate residues. shp migrated diffusely on polyacrylamide gels, which suggested modification by pol ... | 1992 | 1413513 |
| altered glycosylation of membrane glycoproteins associated with human mammary carcinoma. | n-linked sugar chains of normal mammary gland, mammary carcinomas (primary lesion), and axillary lymph node metastases of mammary carcinomas were released from their membrane preparations by hydrazinolysis and their structures were analyzed. fractionation using a datura stramonium agglutinin (dsa)-sepharose column revealed that the metastasized carcinomas contain more than twice as much dsa-binding oligosaccharides as the normal gland, and the primary carcinomas contain an intermediate amount. t ... | 1992 | 1452459 |
| characterization of carbohydrate chains of c1-inhibitor and of desialylated c1-inhibitor. | carbohydrate chains of c1-inhibitor were identified with a binding assay using different lectins. lectins from sambucus nigra (sna) and maackia amurensis (maa) that are specific for sialic acids bound to c1-inhibitor. lectin from datura stramonium (dsa) reacted also with the inhibitor indicating complex and hybrid sugar structures. c1-inhibitor was enzymatically desialylated and reexamined for lectin binding. sna and maa did not react anymore, but in addition to dsa, peanut agglutinin, which can ... | 1992 | 1468580 |
| differential glycosylation and cell surface expression of lysosomal membrane glycoproteins in sublines of a human colon cancer exhibiting distinct metastatic potentials. | changes in the glycosylation of asparagine-linked oligosaccharides have been shown in various tumor cells, including human colon cancer. attempts were made to elucidate the difference in asn-linked oligo-saccharides attached to lysosomal membrane glycoproteins isolated from sublines of human colon carcinoma exhibiting high and low metastatic potentials in nude mice. lysosomal membrane glycoproteins (lamp) 1 and 2 were immunoprecipitated from the cells after labeling with radioactive sugars, and ... | 1992 | 1544942 |
| structures of the asparagine-linked oligosaccharide chains of human von willebrand factor. occurrence of blood group a, b, and h(o) structures. | the asparagine-linked oligosaccharide chains of human von willebrand factor (vwf) purified from pooled plasma were quantitatively liberated from the polypeptide moiety by hydrazinolysis. after n-acetylation, these were fractionated by paper electrophoresis and sequential chromatography on lectin-affinity columns of concanavalin a, phaseolus vulgaris erythrophytohemagglutinin, datura stramonium agglutinin, ricinus communis agglutinin 120, and ulex europaeus agglutinin i and on a bio-gel p-4 colum ... | 1992 | 1577715 |
| purification of a trypanosoma cruzi trypomastigote 60-kilodalton surface glycoprotein that primes and activates murine lymphocytes. | we have purified a glycoprotein with a relative molecular mass of 60 kda and present on the surface of trypanosoma cruzi trypomastigotes and studied its ability to prime and stimulate the proliferation of murine spleen cells. t. cruzi trypomastigote membrane proteins were separated by preparative isoelectrofocusing. a trypomastigote 60-kda surface protein with an isoelectric point of 4.2 was enriched by chromatofocusing and was readily purified in native form to homogeneity by gel filtration on ... | 1992 | 1639469 |
| datura stramonium agglutinin-reactive alpha-fetoprotein isoforms in hepatocellular carcinoma and other tumors. | by means of datura stramonium agglutinin (dsa) affinity electrophoresis, human alpha-fetoprotein (afp) was resolved into five bands, afp-d1, d2, d3, d4 and d5, in order of decreasing mobility. afp-d1, which had no affinity for dsa, comprised more than 84% of the intensity of total afp bands. the percentage of afp-d2 increased marginally in hepatitis and liver cirrhosis with or without hepatocellular carcinoma. afp-d3 increased characteristically in hepatocellular carcinoma and afp-d4, which had ... | 1990 | 1695389 |
| carbohydrate-binding specificity of tetracarpidium conophorum lectin. | the carbohydrate-binding specificity of a novel plant lectin isolated from the seeds of tetracarpidium conophorum (nigerian walnut) has been studied by quantitative hapten inhibition assays and by determining the behavior of a number of oligosaccharides and glycopeptides on lectin-sepharose affinity columns. the tetracarpidium lectin shows preference for simple, unbranched oligosaccharides containing a terminal gal beta 1----4glnac sequence over a gal beta 1----3glcnac sequence and substitution ... | 1991 | 1711034 |
| comparative study of the n-linked oligosaccharides released from normal human esophageal epithelium and esophageal squamous carcinoma. | n-linked sugar chains of normal human esophageal epithelium and esophageal squamous carcinoma were quantitatively released as oligosaccharides from their membrane preparations by hydrazinolysis. after being fractionated by serial lectin column chromatography using concanavalin a-sepharose and datura stramonium agglutinin-sepharose, their structures were elucidated by exoglycosidase digestion in combination with methylation analysis. both normal epithelium and esophageal carcinoma contained bi-, ... | 1990 | 2114390 |
| carbohydrate binding specificities of several poly-n-acetyllactosamine-binding lectins. | the structural requirements for the interaction of the asn-linked poly-n-acetyllactosamine-type oligosaccharide moieties of glycoproteins with various n-acetylglucosamine-binding lectins were investigated by means of affinity chromatography on immobilized lectin-sepharose columns. high molecular weight glycopeptides containing poly-n-acetyllactosamine-type oligosaccharides obtained by pronase digestion of human erythrocyte ghosts were treated with 0.1 m trifluoroacetic acid at 100 degrees c for ... | 1990 | 2152329 |
| granulocytic differentiation of hl-60 cells is associated with increase of poly-n-acetyllactosamine in asn-linked oligosaccharides attached to human lysosomal membrane glycoproteins. | hl-60 cells were induced to differentiate into granulocytic cells by dimethyl sulfoxide, and structures of asn-linked oligosaccharides attached to lysosomal membrane glycoproteins (lamp-1 and lamp-2) were elucidated before and after differentiation. lamp-1 and lamp-2 were immunoprecipitated from the cells after labeling with radioactive sugars, and glycopeptides were prepared. the structures of glycopeptides obtained after serial lectin-affinity chromatography were elucidated by endo-beta-galact ... | 1990 | 2243101 |
| the polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. localization on the peptide backbones. | lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 n-glycans, some of which are modified by poly-n-acetyl-lactosamine. we have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells. polylactosaminoglycan-containing glycopeptides, obtained by trypsin, pepsin, and v8 protease digestion of the glycoproteins, were isolated by datura stramonium agglutinin affinity chromatography, gel fil ... | 1990 | 2243102 |
| the effects of low levels of dietary toxic weed seeds (jimson weed, datura stramonium and sicklepod, cassia obtusifolia) on the relative size of rat liver and levels and function of cytochrome p-450. | preliminary studies and previous work by other investigators have shown that jimsonweed and sicklepod seeds are very toxic. such seeds and others are co-harvested and are often found mingling with commercial grain destined for human consumption. low levels of these seeds were studied here to determine whether such levels would show signs of intoxication in rats by modulating growth, liver weight and relative size, cytochrome p-450 levels and function, and other relevant parameters proven to be s ... | 1990 | 2260115 |
| [clinical effects of an extreme ca overdose in feed for growing piglets]. | in a piggery (approx. 60 sows) nearly all the weaned piglets (about 150) showed signs of poor development, reduced feed intake, increased water consumption, markedly dry and hard faeces, urine drinking, navel suckling and drowsiness over 6-8 weeks. in blood samples of 4 affected piglets a significant hypercalcaemia (values greater than 20 mg/dl)--combined with an at times marked hypophosphataemia--was measured. in the diet offered, a ca content of 56.2 g/kg was estimated. an intoxication due to ... | 1990 | 2377989 |
| decrease in polylactosaminoglycans associated with lysosomal membrane glycoproteins during differentiation of caco-2 human colonic adenocarcinoma cells. | the proportion of labeled polylactosaminoglycans found in glycoproteins decreases during spontaneous differentiation of caco-2 human colonic adenocarcinoma cells to enterocytes in culture (a. youakim and a. herscovics, biochem. j., 247: 299-306, 1987). to identify polylactosaminoglycan-containing glycoproteins, caco-2 cells were incubated with [3h]glucosamine or [3h]fucose, for 24 h, and membrane glycoproteins solubilized with 0.5% nonidet p-40 were fractionated by affinity chromatography on dat ... | 1989 | 2582431 |
| toxicological evaluation of jimson weed (datura stramonium) seed. | diets containing 0.5, 1.58 and 5.0% jimson weed seed were fed to male and female rats (20/group) in a 90-day subchronic feeding study. the alkaloid content was 2.71 mg atropine and 0.66 mg scopolamine/g of seed. gross clinical observations, body weights and feed and water intakes were recorded weekly. tear production and pupil dilation measurements were made throughout the study. at 90 days, all of the animals were autopsied and clinical-chemistry analyses, complete haematology and bone-marrow e ... | 1989 | 2792973 |
| light and electron microscopic detection of (3 gal beta 1,4 glcnac beta 1) sequences in asparagine-linked oligosaccharides with the datura stramonium lectin. | the datura stramonium lectin recognizes with high affinity the disaccharide n-acetyllactosamine (gal beta 1,4 glcnac). we have developed a highly specific cytochemical affinity technique in which an ovomucoid-gold complex serves as second step reagent for the visualization of this lectin bound to reactive sequences present in tissue sections. the lectin binding sites were detected in semithin and ultrathin sections of aldehyde-fixed and low temperature lowicryl k4m embedded tissues. for light mi ... | 1989 | 2807997 |
| comparative studies of the sugar chains of aminopeptidase n and dipeptidylpeptidase iv purified from rat kidney brush-border membrane. | asparagine-linked sugar chains of rat kidney aminopeptidase n and dipeptidylpeptidase iv were investigated comparatively. oligosaccharides released from the two enzymes by hydrazinolysis were fractionated by paper electrophoresis, serial chromatographies on columns of immobilized aleuria aurantia lectin, concanavalin a, phytohemagglutinin e4, and datura stramonium agglutinin, and bio-gel p-4 (less than 400 mesh) column chromatography. structures of oligosaccharides in each fraction were assumed ... | 1988 | 2902877 |
| two chromosomal loci involved in production of exopolysaccharide in agrobacterium tumefaciens. | the chromosomal locus psca (exoc) of agrobacterium tumefaciens lba4301 has been cloned by complementation of the avirulent and exopolysaccharide (eps)-deficient mutant lba4301 psca. we have also identified a new locus, termed psda (polysaccharide depression) and located 16 kilobases from psca in the a. tumefaciens chromosome, that negatively affects eps production when it is present in more than one copy in a. tumefaciens lba4301. subcloning, transposon mutagenesis, and transcriptional analysis ... | 1989 | 2921249 |
| differentiation-associated decrease in the proportion of fucosylated polylactosaminoglycans of caco-2 human colonic adenocarcinoma cells. | caco-2 cells are human colonic adenocarcinoma cells which can differentiate spontaneously into enterocytes when maintained confluent for extended periods of time. cells kept in culture for 4 days (rapidly growing), 7-9 days (early confluence) and 19-22 days (late confluence) were incubated for 24 h with l-[5,6-3h]fucose or d-[6-3h]glucosamine in order to examine the changes in glycoprotein carbohydrate structure that occur during this differentiation. labelled glycopeptides obtained by exhaustiv ... | 1987 | 3122722 |
| estrogen preferentially stimulates lactosaminoglycan-containing oligosaccharide synthesis in mouse uteri. | the effects of steroid hormones on the synthesis of lactosaminoglycan (lag)-containing oligosaccharides by mouse uteri are reported. the uterine lag-containing oligosaccharides were degraded partially by pseudomonas endo-beta-galactosidase, releasing an oligosaccharide of the apparent structure: gal beta----n-acetylglucosaminyl(----n-acetylgalactosaminyl)beta 1,3----galactose. a larger fraction of the lag-containing oligosaccharides bound to pokeweed mitogen than to datura stramonium lectin, sug ... | 1988 | 3123490 |
| datura lectin is both an anti-mitogen and a co-mitogen acting synergistically with phorbol ester. | the lectins from datura stramonium, lycopersicon esculentum, and solanum tuberosum are structurally related and possess a similar carbohydrate specificity, yet the datura lectin is mitogenic for human lymphocytes while the other two are not. however, the datura lectin was found to antagonize blast transformation induced by purified protein derivative (ppd), even within the concentration range at which it was optimally mitogenic on its own. the presence of a submitogenic concentration of the phor ... | 1988 | 3124260 |
| immobilized datura stramonium agglutinin column chromatography, a novel method to discriminate the urinary hcgs of patients with invasive mole and choriocarcinoma from those of normal pregnant women and patients with hydatidiform mole. | datura stramonium agglutinin (dsa) binds the asparagine-linked sugar chains commonly found in the human chorionic gonadotropin (hcg) purified from urine of patients with invasive mole or choriocarcinoma, but not in that of normal pregnant women or patients with hydatidiform mole. with the use of an immobilized dsa column, a novel method to discriminate urinary hcgs from various trophoblastic diseases was developed. | 1988 | 3130349 |
| purification and characterization of the d2-dopamine receptor from bovine anterior pituitary. | the d2-dopamine receptor from bovine anterior pituitary has been purified approximately 33,000-fold to apparent homogeneity by sequential use of affinity chromatography on immobilized carboxymethyleneoximinospiperone-sepharose, datura stramonium lectin-agarose, and hydroxylapatite chromatography. the purification yields a single polypeptide band of mr approximately 120,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed by labeling with radioiodinated bolton-hunter reagent, ... | 1988 | 3143721 |
| [mannan sulfates--inducers of plant resistance to viral infection]. | mannan sulphates (ms) synthesized on the basis of extracellular linear mannan (lm) of rhodotorula rubra induce resistance of immune-580 tobacco and thornapple (datura stramonium) to tobacco mosaic virus (tmv). the resistance is manifested in a decrease in the number and/or size of viral local lesions (ll) in ms-treated (mg/ml) leaf halves. the reduction in the ll size does not seem to be due to the direct inhibition of tmv multiplication by the polysaccharide as the virus accumulation in the tis ... | 1988 | 3247692 |
| accessory cell paradox: monocytes enhance or inhibit lectin-mediated human t-lymphocyte proliferation depending on the choice of mitogen. | monocytes suppressed the mitogenic response of human t lymphocytes to the lectin from datura stramonium but enhanced the mitogenic response of the same lymphocytes to wheat germ agglutinin under identical culture conditions. the inhibitory action was probably cell-mediated, but was not the result of cytotoxicity. the stimulative activity could be replaced by cell-free conditioned medium. | 1988 | 3261885 |
| identification of group b streptococcal antigen with lectin-bound polystyrene particles. | the lectin of the tomato, lycopersicon esculentum, or of the potato, solanum tuberosum, can be passively coupled to amide-modified polystyrene spheres to be used as a detection reagent for the specific identification of group b streptococcal cultures grown in selective or nonselective todd-hewitt broth for 5 and 4 h, respectively. agglutination occurred when the lectin reagents were allowed to react with either the cell suspension, clarified broth, or antigen extracts from group b streptococci g ... | 1987 | 3301888 |
| inhibition of yeast binding to mouse peritoneal macrophages by wheat germ agglutinin: a novel effect of the lectin on phagocytic cells. | the ability of wheat germ agglutinin (wga) to enhance the binding of bacteria and tumor cells to phagocytic cells, and to induce the killing of tumor cells by macrophages and monocytes, is well established. we observed, however, that wga inhibits the binding to and phagocytosis of yeast cells by thioglycolate-elicited murine peritoneal macrophages. in order to follow these processes rapidly, the yeasts were labeled with congo-red and their binding to the macrophages was measured spectrophotometr ... | 1987 | 3318827 |
| polylactosaminoglycan modification of a small integral membrane glycoprotein, influenza b virus nb. | the structure of the carbohydrate components of nb, the small integral membrane glycoprotein of influenza b virus, was investigated. the carbohydrate chains of nb are processed from the high-mannose form (nb18) to a heterogeneous form of much higher molecular weight, designated nbp. selection of this carbohydrate-containing form of nb with datura stramonium lectin, its susceptibility to digestion by endo-beta-galactosidase, and determination of the size of nbp glycopeptides by gel filtration chr ... | 1988 | 3367907 |
| secretion of a lactosaminoglycan-containing glycoprotein by peri-implantation sheep conceptuses. | sheep conceptuses from day 16 of pregnancy were cultured in the presence of [3h]glucosamine and [14c]leucine and a high-molecular-weight glycoprotein (hmwg) secreted into the culture medium was purified by a combination of anion-exchange and gel filtration chromatography. the hmwg was found to have a molecular weight between 800,000 and 900,000 and to be highly resistant to digestion with pronase. characteristics of the carbohydrate portion of the purified glycoprotein were examined by selective ... | 1988 | 3390467 |
| carbohydrate binding properties of complex-type oligosaccharides on immobilized datura stramonium lectin. | the carbohydrate binding specificity of datura stramonium agglutinin was studied by analyzing the behavior of a variety of complex-type oligosaccharides on a d. stramonium agglutinin-sepharose column. oligosaccharides which contain gal beta 1----4glcnac-beta 1----4(gal beta 1----glcnac beta 1----2)man units are retarded in the column so long as the pentasaccharide unit is not substituted by other sugars. oligosaccharides which contain unsubstituted gal beta 1----4glcnac beta 1----6(gal beta 1--- ... | 1987 | 3805046 |
| heterophile agglutinins with a broad-spectrum specificity. vi. the nature of cell surface receptors for the agglutinins present in seeds of amaranthus caudatus, maclura aurantica, datura stramonium, viscum album, phaseolus vulgaris and moluccella laevis. | | 1970 | 4249809 |
| effect of actinomycin d on potato virus x multiplication in datura stramonium leaves. | | 1973 | 4350225 |
| isolation of the tumor-inducing rna from oncogenic and nononcogenic agrobacterium tumefaciens. | two rna fractions have been isolated and purified from both oncogenic and nononcogenic strains of agrobacterium tumefaciens. both rnas are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of datura stramonium plants. one of these rna fractions was found to be bound to an rna-directed dna polymerase, while the other was associated with the bacterial dna. physical evidence suggests that both are single stranded and small in size; linear sucrose gra ... | 1974 | 4525450 |
| [distribution of potato virus x in a tissue culture of jimsonweed (datura stramonium)]. | | 1972 | 4667028 |
| an electron microscope study of the distribution of tomato spotted wilt virus in systemically infected datura stramonium leaves. | | 1970 | 4923022 |
| [induction of tumors by the thermostable endotoxin of agrobacterium tumefaciens on datura stramonium l]. | | 1971 | 5129961 |
| ultrastructure of potato and datura stramonium plant cells infected with potato virus x. | | 1969 | 5784848 |
| visualization of tobacco mosaic virus in local lesions of datura stramonium. | | 1965 | 5827068 |
| carbohydrate binding studies on the lectin from datura stramonium seeds. | the carbohydrate-binding properties of the datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. the dimeric lectin (mr = 86,000) possesses two carbohydrate-binding sites for n,n'n",n"'- tetraacetylchitotetritol /mol protein, with an apparent ka = 8.7 x 10(3) m-1 at 4 degrees c. whereas fetuin and orosomucoid reacted poorly with the datura lectin, the asialo derivatives of the ... | 1984 | 6203486 |
| datura intoxication in riyadh. | cases of stramonium poisoning due to deliberate abuse by young adults have been widely reported. there are also a few reports of accidental poisoning in children from contaminated food. the taste and shape of datura stramonium makes it unattractive to both man and animals and, therefore, ingestion by children in ignorance of its toxicity is rare. three such cases are described in this paper. in saudi arabia stramonium abuse is unheard of and poisoning of children as a result of food contaminatio ... | 1984 | 6710568 |
| the distribution of repeating [gal beta 1,4glcnac beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines bw5147 and phar 2.1. | the occurrence and distribution of the repeating disaccharide [gal beta 1,4glcnac beta 1,3] in the different types of asn-linked oligosaccharides in mouse lymphoma bw5147 cells have been studied. glycopeptides were prepared from cells grown in medium containing [6-3h]galactose, and the bi-, tri-, and tetraantennary asn-linked oligosaccharides were fractionated by serial lectin affinity chromatography on concanavalin a-sepharose, pea lectin -sepharose, leukoagglutinating phytohemagglutinin-agaros ... | 1984 | 6725252 |
| toxicity of thornapple (datura stramonium l) seeds to the pig. | experimental work reported here, together with a review of the available published work, indicates an intake of datura stramonium seeds equivalent to a dose of about 1.5 mg alkaloid per kg body-weight as a threshold level for the expression of mild toxicity symptoms in the pig. the pig can very effectively reject whole seeds in the diet and whole seeds ingested may pass through the gut intact. thornapple seems far less likely to cause poisoning in the housed pig than is generally believed. the s ... | 1981 | 7222435 |
| specificity of twelve lectins towards oligosaccharides and glycopeptides related to n-glycosylproteins. | glycopeptides and oligosaccharides of either the n-acetyllactosaminic or the oligomannosidic type derived from glycoproteins containing the n-glycosylamine linkage were used to define the specificity of different lectins (concanavalin a, lens culinaris agglutinin, vicia faba agglutinin, pisum sativum agglutinin, ricinus communis agglutinins, soybean agglutinin, wheat germ agglutinin, solanum tuberosum agglutinin, datura stramonium agglutinin, lotus tetragonolobus agglutinin, ulex europeus agglut ... | 1981 | 7262089 |
| toxicity of datura stramonium to sheep and goats. | the toxicity of daily oral administration of datura stramonium to desert sheep and nubian goats was studied. the main clinical signs were disturbances in locomotion, fasciculation, hyperesthesia, rapid respiration, and reduced water intake. there were increases in the concentrations of aspartate amino-transferase and ammonia and decreases of total protein and magnesium in the serum of datura-poisoned animals. in this study desert sheep appeared to be more susceptible to the effects of datura str ... | 1981 | 7314411 |
| absence of arthrogryposis in newborn hampshire pigs from sows ingesting toxic levels of jimsonweed during gestation. | no congenital arthrogryposis or other deformities were found in newborn hampshire pigs born to sows administered datura stramonium (jimsonweed) during the second or third months of pregnancy at levels producing marked clinical signs of toxicity in the dams. the results suggest that jimsonweed is not teratogenic in pigs, contrary to epidemiologic speculation by other workers. | 1981 | 7336562 |
| [the effect of steroids on organ-cultured porcine trabecular meshwork--an ultrastructural, biochemical, and lectin histochemical study]. | the effect of corticosteroid administration on porcine trabecular meshwork (ptm) was studied by ultrastructural and biochemical analysis. electron microscopic observations showed an increase of elastic fibers in ptm treated with steroids. two-dimensional polyacrylamide gel electrophoresis with silver staining showed an increase of proteins with molecular weights of 52 kda, 66 kda and 70 kda. the 66 kda protein was assumed to be a glycoprotein containing n-acetyl-d-glucosamine, as it was stained ... | 1995 | 7484510 |
| the use of lectins in monitoring degradation of oligosaccharide chains in mucin by oral streptococci. | the ability of utilize mucin oligosaccharides as sources of carbohydrate and energy is believed to be an important mechanism in the ecology of oral streptococci. in this study we have used digoxigenin-labelled lectins of various specificities to monitor changes in the nonreducing end groups of oligosaccharide chains following their degradation by streptococcus oralis ny 586 and streptococcus sanguis ny 584. the reaction of degraded mucin with peanut lectin, that recognizes the core disaccharide ... | 1994 | 7520835 |
| cytokeratin peptide sfgsgfgggy mimics n-acetyl-beta-d-glucosamine in reaction with antibodies and lectins, and induces in vivo anti-carbohydrate antibody response. | recently, we found that human anti-glcnac mabs reacted with keratin from human skin and recognized specific peptide epitopes of human cytokeratin 14. our data demonstrate that anti-keratin ab responses in mice might be driven by ags bearing terminal o-linked glcnac residues. to determine if mouse anti-glcnac mabs recognized peptide epitopes of keratin, several mouse anti-glcnac mabs were reacted with a panel of overlapping synthetic decapeptides of the entire amino acid sequence of human cytoker ... | 1994 | 7527445 |
| involvement of n-acetyl-lactosamine-containing sugar structures in the liver metastasis of mouse colon carcinoma (colon 26) cells. | histochemical aspects of the process of experimentally induced metastasis were examined by light and electron microscopy with labelled lectins employed as a probe. mouse colon carcinoma cells (colon 26) were injected into the spleen of balb/c mice and liver metastasis was induced. among the lectins tested, erythrina cristagalli agglutinin (eca) stained the metastasized colon 26 cells strongly compared with the heterogeneous and faint staining in non-metastasized tumour foci in the spleen or in t ... | 1994 | 7532448 |
| datura stramonium agglutinin released histamine from rat peritoneal mast cells that was inhibited by pertussis toxin, haptenic sugar and n-acetylglucosamine-specific lectins: involvement of glycoproteins with n-acetylglucosamine residues. | the n-acetyl glucosamine (glcnac)-specific lectin datura stramonium agglutinin (dsa) rapidly and sugar-specifically released histamine from rat peritoneal mast cells, and pertussis toxin (iap) inhibited it, suggesting that dsa activated mast cells via an iap-sensitive g protein pathway. the additive effects of dsa and basic secretagogues such as compound 48/80 that activate iap-sensitive g protein directly suggest that they shared the same mechanism of action including involvement of the iap-sen ... | 1994 | 7532733 |
| an initial signal of activation of rat peritoneal mast cells stimulated by datura stramonium agglutinin: a confocal fluorescence microscopic analysis of intracellular calcium ion and cytoskeletal assembly. | a confocal fluorescence microscope using fluo-3 and 9-(dicyanovinyl)- julolidine (dcvj) was used to study the mast cell activation by the n-acetyl glucosamine oligomer specific lectin datura stramonium agglutinin (dsa) and inhibition by antagonist lectins having affinity to n-acetyl glucosamine (glcnac). dsa induced a transient increase in intracellular free calcium concentration ([ca2+]i) followed by cytoskeletal disassembly and reassembly in rat peritoneal mast cells. these changes induced by ... | 1994 | 7532734 |
| glycosylation of beta-1 integrins in b16-f10 mouse melanoma cells as determinant of differential binding and acquisition of biological activity. | studying b16-f10 cells we could identify beta-1 integrins as laminin, fibronectin and collagen receptors. gradient ionic strength elution analysis of affinity chromatography showed differential interactions between laminin-binding beta-1 integrins (two beta-1 polypeptides of 105 and 120 kda) and fibronectin and collagen-binding beta-1 integrins (elution of one major beta-1 polypeptide of 120 kda) and their respective ligands. to evaluate this diversity we submitted b16-f10 extracts to ief and sd ... | 1995 | 7537256 |
| glycans with n-acetyllactosamine type 2-like residues covering adult schistosoma mansoni, and glycomimesis as a putative mechanism of immune evasion. | glycans at the surface of adult schistosoma mansoni were investigated with gold-labelled lectins. the fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. male and female worms were entirely covered with glycans that intensely bound lectins from erythrina cristagalli and datura stramonium, suggesting that galactose(beta 1-4)n-acetylglucosa ... | 1995 | 7567100 |
| glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat. | alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. the binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using histochemical methods. proteoglycan distribution, assessed by conventional staining procedures, was not associated with lectin bind ... | 1995 | 7591987 |
| prostaglandin e2 alters terminal glycosylation of high molecular weight glycoproteins, released by pig gastric mucous cells in vitro. | the gastric mucus layer consists of high molecular weight glycoproteins (hmg). e-type prostaglandins (pgs) stimulate total hmg release from isolated gastric mucous cells. we determined the effects of pge2 on hmg glycosylation. pig gastric mucous cells were cultured for 20 h with 1 mumol/l pge2. released hmg were isolated by gel chromatography and periodic acid-schiff (pas)-positive sugars and protein-bound [14c]glcnac were determined. monosaccharides terminally linked to hmg oligosaccharide chai ... | 1995 | 7630922 |
| major organ-specific glycoproteins in isolated brain and kidney membranes identified as na,k-atpase subunits by combined glycan-, lectin-, and immunoblotting. | in the present work combined glycan-, lectin-, and immunoblotting of isolated brain and kidney membranes shows that the alpha and beta subunits of na,k-atpase are the most abundant glycoproteins. further, datura stramonium and galanthus nivalis agglutinins recognize the na,k-atpase subunits in a mutually exclusive manner in membranes from human, rabbit and rat brain or human, rabbit, rat, pig and dog kidney indicating the presence of species-independent organ-typical glycoforms. the glycosylatio ... | 1995 | 7647289 |
| characterization of glycoconjugate expression during development of meckel's cartilage in the rat. | the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ... | 1995 | 7717533 |
| expression of membrane glycoconjugates on sheep lung endothelium. | carbohydrates play an important role in both the regulation and expression of endothelial cell surface molecules. | 1995 | 7723284 |
| maackia amurensis agglutinin discriminates between normal and chronic leukemic human lymphocytes. | altered glycosylations of cell surface glycoproteins often accompany malignant transformation and lectins are useful for probing these alterations. lymphocytes exhibit characteristic surface glycoproteins which serve as markers of cell status and development. the present work was undertaken to compare, on blots, the binding characteristics of membranes isolated from normal peripheral blood lymphocytes and chronic lymphatic leukemia cells to five different lectins, from datura stramonium, maackia ... | 1995 | 7733985 |
| characterization of human arylsulfatase a glycans. | despite numerous studies on arylsulfatase a, the structure of the glycans present in each of its two subunits has not been determined. this is important because the carbohydrate component of human arylsulfatase a synthesized in tumor tissues and transformed cells has been shown to undergo apparent changes. this study elucidates some of their major features. glycan chain analysis of native and deglycosylated arylsulfatase a as well as its subunits was performed with the use of a glycan differenti ... | 1994 | 7890120 |
| bovine retinal rod guanyl cyclase represents a new n-glycosylated subtype of membrane-bound guanyl cyclases. | the molecular properties of retinal rod guanyl cyclase were investigated. peptides were derived from a 112-kda protein previously identified as the particulate bovine retinal rod guanyl cyclase. the peptides showed 100% identity to the deduced amino acid sequence of the cloned human retina-specific membrane guanyl cyclase, whereas identity to the members of the natriuretic peptide receptor guanyl cyclases was 14-59%. the 112-kda protein was further purified by a new approach using wheat-germ agg ... | 1994 | 7912673 |
| orf c4 of tomato leaf curl geminivirus is a determinant of symptom severity. | a tomato leaf curl virus (tlcv) mutant has been constructed in vitro that contains t-to-c replacements at nucleotides 2457 and 2463 within the c4 open reading frame (orf). the mutations destroy the two possible initiator aug codons for the c4 orf without disrupting the coding capacity of the c1 orf which entirely overlaps the c4 orf. agroinoculation of the c4 mutant tlcv into three alternative experimental hosts for the virus (datura stramonium, lycopersicon esculentum, and nicotiana tabacum) gi ... | 1994 | 7941358 |
| characterization of o-glycan moieties of the 210 and 240 kda pig intestinal receptors for escherichia coli k88ac fimbriae. | the porcine brush border glycoproteins of 210 and 240 kda, recognized by escherichia coli k88ac fimbriae, contained o-linked oligosaccharides. the carbohydrate moieties were analysed by deglycosylation, lectin-binding and agglutination assays. neuraminidase susceptibility of the 210 kda receptor suggested that a sialoglycoprotein may act as receptor for the k88ac fimbriae. in contrast, k88ac-binding to the 210 and 240 kda glycoproteins totally disappeared after fucosidase treatment, indicating t ... | 1994 | 7952196 |
| agglutination of "streptococcus milleri" by lectins. | the agglutination of 218 clinical isolates and three atcc type strains of "streptococcus milleri" was tested with 25 different lectins from plants and fungi. an agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. after trypsinisation of the bacteria, 109 strains yielded a positive reaction and after boiling the bacterial cells at ph2, 218 isolates were agglutinated. as an overall result of our experiments with untreated, trypsinised and bo ... | 1994 | 8006940 |
| characterization of a novel family of ciliary body glycoproteins. | to isolate and characterize ciliary body epithelial antigens reactive with a monoclonal antibody, 2b4.14.1. | 1994 | 8045720 |
| inhibition of lectin-mediated ovarian tumor cell adhesion by sugar analogs. | adhesion of a-121 human ovarian carcinoma cells to extracellular matrix is partly mediated via interaction between galaptin, an endogenous beta-galactoside-binding lectin present in extracellular matrix, and specific cell surface carbohydrate receptors identified as lysosomal associated membrane proteins, lamp-1 and lamp-2. in this study, we report that adhesion of human ovarian carcinoma cells to polystyrene plates coated with polymerized human splenic galaptin can be inhibited by polyclonal an ... | 1994 | 8077232 |
| alteration by transforming growth factor-beta 1 of asparagine-linked sugar chains in glucose transporter protein in swiss 3t3 cells. | glut1 protein in swiss 3t3 cells is a 55-kda glycoprotein with an n-linked oligosaccharide chain. we previously showed that the 65-kda glut1 protein with modulated glycosylation was induced by transforming growth factor-beta 1 (tgf-beta 1) in swiss 3t3 cells. to further investigate the altered structures of these sugar chains, the membrane glycoproteins solubilized with triton x-100 were fractionated by lectin-affinity chromatography. the 55-kda glut1 in control and tgf-beta 1-treated cells show ... | 1994 | 8167156 |
| iron binding to human lactoferrin alters reactivity of the protein with plant lectins. | binding of fe by human apolactoferrin results in altered reactivity of the glycoprotein with plant lectins. reaction with wheat germ agglutinin (wga) and peanut agglutinin (pna) was abolished with fe binding. reaction with the lectins from datura stramonium (dsa) and aleuria aurantia (aaa) was significantly reduced but not fully abolished on fe binding, while reaction with the artocarpus integrifolia lectin (jacalin) and sambucus nigrabark (sna) was not changed at all. loss of wga reactivity occ ... | 1993 | 8240344 |
| purification of a functional receptor for clostridium difficile toxin a from intestinal brush border membranes of infant hamsters. | a receptor for clostridium difficile toxin a was purified from brush border membranes (bbms) from the small intestine of infant hamsters. the bbms were solubilized with triton x-114, and the solubilized receptor was purified with use of a toxin a immobilized affinity-chromatography column and differential temperature elution. sds-page and silver staining of the purified receptor revealed numerous high-molecular-weight bands. however, ligand blotting analysis with 125i-toxin a used as the probe i ... | 1993 | 8324123 |
| antinutritive effects of wheat-germ agglutinin and other n-acetylglucosamine-specific lectins. | incorporation of n-acetylglucosamine-specific agglutinins from wheat germ (triticum aestivum; wga), thorn apple (datura stramonium) or nettle (urtica dioica) rhizomes in the diet at the level of 7 g/kg reduced the apparent digestibility and utilization of dietary proteins and the growth of rats, with wga being the most damaging. as a result of their binding and endocytosis by the epithelial cells of the small intestine, all three lectins were growth factors for the gut and interfered with its me ... | 1993 | 8399111 |
| two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor. | in the biosynthetic pathway of tropane alkaloids, tropinone reductase (ec 1.1.1.236) (tr)-i and tr-ii, respectively, reduce a common substrate, tropinone, stereospecifically to the stereoisomeric alkamines tropine and pseudotropine (psi-tropine). cdna clones coding for tr-i and tr-ii, as well as a structurally related cdna clone with an unknown function, were isolated from the solanaceous plant datura stramonium. the cdna clones for tr-i and tr-ii encode polypeptides containing 273 and 260 amino ... | 1993 | 8415746 |
| production of a monoclonal antibody against cell-surface glycoprotein of guinea pig adrenocortical cells. | a monoclonal antibody (mab) that reacted with the cell-surface antigens of adrenocortical cells was generated against cell suspensions from guinea pig adrenal glands. cell-surface membranes of the adrenocortical cells in all zones, i.e., zona glomerulosa, zona fasciculata, and zona reticularis, were labeled with the antibody. adrenal medulla remained unlabeled. immunoelectron microscopy showed that entire plasma membranes, i.e., plasma membranes between adjacent cells and free cell-surface membr ... | 1993 | 8419461 |
| restoration of pathogenicity of avirulent xanthomonas oryzae pv. oryzae and x. campestris pathovars by reciprocal complementation with the hrpxo and hrpxc genes and identification of hrpx function by sequence analyses. | the molecular basis of pathogenesis by xanthomonas oryzae pv. oryzae has been partly elucidated by the identification of a gene, hrpxo, required for bacterial blight on rice. a mutation in hrpxo results in the loss of pathogenicity on rice and the loss of hypersensitivity on nonhosts such as datura stramonium and radishes. pathogenicity and its ability to cause the hypersensitive reaction is restored by complementing the mutant with the heterologous hrpxc gene derived from x. campestris pv. camp ... | 1993 | 8458844 |
| biological activities of fumonisins, mycotoxins from fusarium moniliforme, in jimsonweed (datura stramonium l.) and mammalian cell cultures. | fumonisins a1, a2, b1, b2 and b3 are a series of mycotoxins produced by strains of fusarium moniliforme. fumonisins are hydroxylated long-chain alkylamines esterified with propanetricarboxylic acid moieties that represent approximately half the mol. wt of the toxins. the a-series fumonisins are n-acetylated, whereas the b series contains free amino groups. hydrolytic removal of the propanetricarboxylic acid moieties from fumonisins b1 and b2 yields the corresponding aminopentols, ap1 and ap2, re ... | 1993 | 8470138 |
| histochemical demonstration of different types of poly-n-acetyllactosamine structures in human thyroid neoplasms using lectins and endo-beta-galactosidase digestion. | blood-group-related antigens expressed in papillary carcinomas and other types of neoplasm of the human thyroid glands have been shown to be carried by poly-n-acetyllactosamines containing a linear domain susceptible to endo-beta-galactosidase digestion. to make clear more precisely the backbone poly-n-acetyllactosamine structures, labelled lectins specific to different types of these structures and specific to core structures with beta 1-6glcnac branching of n- and o-linked glycoproteins were e ... | 1995 | 8550383 |
| host and viral factors determine the dispensability of coat protein for bipartite geminivirus systemic movement. | geminiviruses have unique, twinned icosahedral particles which encapsidate circular single-stranded dna. their genomes are composed of either one or two dna segments. monopartite geminiviruses absolutely require a functional coat protein (cp) for infectivity, whereas bipartite geminivirus cp null mutants can infect plants systemically. however, we show here that a cp mutant of the bipartite tomato golden mosaic virus (tgmv), which can infect nicotiana benthamiana systemically, is confined to the ... | 1996 | 8615033 |
| molecular cloning and functional identification of a plant ornithine decarboxylase cdna. | a cdna for a plant ornithine decarboxylase (odc), a key enzyme in putrescine and polyamine biosynthesis, has been isolated from root cultures of the solanaceous plant datura stramonium. reverse transcription-pcr employing degenerate oligonucleotide primers representing conserved motifs from other eukaryotic odcs was used to isolate the cdna. the longest open reading frame potentially encodes a peptide of 431 amino acids and exhibits similarity to other eukaryotic odcs, prokaryotic and eukaryotic ... | 1996 | 8660289 |
| [serious plant poisonings in switzerland 1966-1994. case analysis from the swiss toxicology information center]. | to analyze the types, frequency and severity of plant poisonings in switzerland over 29 years. | 1996 | 8711457 |
| benzalkonium chloride inhibited the histamine release from rat peritoneal mast cells induced by bradykinin and glcnac oligomer-specific lectin datura stramonium agglutinin, but heparin did not. | 1. benzalkonium chloride (bac) inhibited the histamine release from rat peritoneal mast cells induced by synthetic cationic polymers (compound 48/80 and pei6), bradykinin, des-arg1-bradykinin and des-arg9-bradykinin and datura stramonium agglutinin (dsa). 2. the anionic polymers heparin, de-n-sulfated heparin, poly-aspartic acid and poly-glutamic acid dose dependently inhibited the histamine release induced by cationic polymers, suggesting counteraction between anions and cations. 3. inhibition ... | 1996 | 8742508 |
| cellular heterogeneity in the membrana granulosa of developing rat follicles: assessment by flow cytometry and lectin binding. | the hormone-mediated maturation of ovarian follicles is apparently accompanied by position-specific differentiation of cells of the membrana granulosa. we have assessed the extent of this cellular heterogeneity by flow cytometry using a variety of fluorescein isothiocyanate-labeled lectins as probes. follicular development was stimulated in immature rats by treatment with either diethylstilbestrol (des) or equine cg (ecg). lectin binding to monodispersed rat granulosa cells was then analyzed by ... | 1996 | 8770935 |
| glycoconjugates of the human trabecular meshwork: a lectin histochemical study. | twelve specimens of resin-embedded human trabecular meshwork were probed with a panel of 21 biotinylated lectins, using an avidin-biotin peroxidase revealing system, in order to determine the normal pattern of saccharide expression in this tissue. high-mannose, intermediate and hybrid n-linked glycans, and complex n-linked bisected and non-bisected bi/tri-antennate glycans, as shown by the binding of canavalia ensiformis (cona), pisum sativum (psa), lens culinaris (lca) agglutinins and phaseolus ... | 1995 | 8787965 |
| further characterization of ige-binding antigens in horse dander, with particular emphasis on glycoprotein allergens. | ige-binding components in an extract of horse dander were analyzed, especially with regard to the glycoprotein allergens. after sds-page under reducing conditions and blotting, several of the glycoprotein ige-binding components, including two distinct bands of 27 and 31 kda, were detected. together with several other bands, they were shown to bind to the lectins sambucus nigra agglutinin (sna) and datura stramonium agglutinin (dsa), indicating terminal sialic acid linked alpha 2 --> 6 to galacto ... | 1996 | 8899112 |
| sialidase activity in culture fluid of chinese hamster ovary cells during batch culture and its effects on recombinant human antithrombin iii integrity. | sialidase activity in cell-free supernatant of batch-cultivated chinese hamster ovary (cho) cells producing human recombinant antithrombin iii (rhat iii) was monitored during cultivation using 4-methylumbelliferyl substrate and hplc for free sialic acid determination. supernatant sialidase as well as lactate dehydrogenase activity increased significantly during batch growth. the enhanced number of dead cells correlated with increasing sialidase activity which seemed to be principally due to cell ... | 1996 | 8987482 |
| isolation of highly purified type a spermatogonia from prepubertal rat testis. | we have developed a new method that allows isolation of highly purified type a spermatogonia from prepubertal rats. the procedure is based on the maximal release of spermatogonia from the seminiferous epithelium obtained by the complete enzymatic digestion of the tubular basal lamina, followed by removal of contaminating somatic cells through adhesion to plastic dishes coated with the lectin datura stramonium agglutinin and fractionation on a discontinuous percoll gradient. the cell suspension o ... | 1996 | 9016402 |
| increased expression of highly branched n-glycans at cell surface is correlated with the malignant phenotypes of mouse tumor cells. | three nih3t3 transformants, mtag, mtpy, and mt1, which grow similarly in soft agar media, showed remarkable differences in athymic mice: mtag grew more rapidly than mtpy, whereas mt1 and nih3t3 did not, and only mtag metastasized in lung. structural analysis of n-glycans from plasma membrane glycoproteins revealed that each sample contains similar amounts of n-glycans, but the relative amounts of 2,6-branched tri- and tetra-antennary oligosaccharides prominently increase and the relative amounts ... | 1997 | 9067274 |
| the complex-type oligosaccharide binding lectin datura stramonium agglutinin detects type ii a muscle fibres in the branchial biceps from man and cat. | complex-type oligosaccharides were detected in the sarcoplasm of muscle fibres from cat and human biceps using lectins and anticarbohydrate antibodies. the lectin datura stramonium agglutinin strongly stained type ii a fibres as identified by myosin atpase activity after alkaline and acid preincubation. in contrast, all muscle fibres showed a moderate coarse granular staining after incubation with tetracarpidum conophorum agglutinin and telfairia occidentalis agglutinin which recognize tri-anten ... | 1997 | 9147991 |
| molecular characterization of a subgroup i geminivirus from a legume in south africa. | a south african geminivirus for which we propose the name bean yellow dwarf virus (beydv) has been isolated from french bean (phaseolus vulgaris cv. bonus) showing stunting, chlorosis and leaf curl symptoms. a full-length cloned copy of the viral genome produced characteristic symptoms of the disease when reintroduced into french bean by agroinoculation, and was systemically infectious in nicotiana benthamiana, n. tabacum, lycopersicon esculentum, datura stramonium and arabidopsis thaliana. beyd ... | 1997 | 9267015 |
| [thorn apple poisoning]. | a 20-year-old man presented with signs of thornapple intoxication: restlessness, disorientation, hallucinations, euphoria, and furthermore dry and red skin and symmetrical dilation of the pupils. thornapple intoxication mimics atropine intoxication. thorn apples (datura stramonium) are seemingly becoming popular as a hallucinogenic drug. when the symptoms are mild gastric lavage leaving active charcoal in the stomach afterwards will suffice. in case of severe symptoms treatment with physostigmin ... | 1997 | 9273454 |
| expression and characterization of a lactosaminoglycan-carrying glycoprotein of zajdela hepatoma cell surface--structural analysis of the carbohydrate moiety. | in poorly differentiated hepatoma cells, a glycoprotein carrying lactosaminoglycans is identified, and the structure of its glycan moiety is proposed. after membrane solubilization, protein fractionation by gel filtration, and electroelution, this glycoprotein (gpiii) was identified by its affinity for datura stramonium lectin and its content in large glycopeptides. as shown by page, gpiii has an apparent molecular mass of 100 kda and is highly glycosylated (36%). it appears as an integral membr ... | 1997 | 9288935 |
| differential binding properties of gal/galnac specific lectins available for characterization of glycoreceptors. | differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty gal and/or galnac specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of galnac alpha 1--> ... | 1997 | 9343930 |