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use of plastome and nuclear mutants of higher plants to study the genetic control of plastid formation and function.we conducted comparative biochemical and electron-microscopic studies of several types of plastome and nuclear mutants of antirrhinum majus and pelargonium zonale. it was shown that specific blocking of the photosynthetic reaction occurs in plastome mutants of a. majus; photosystem ii was found to be damaged in the en:alba-1 mutant and photo-system i was affected in the en:viridis-1 mutant. the plastid mutations in these mutants caused loss of certain soluble lamellar proteins and pigment--prote ...19751124432
plastid ribosome deficient mutants of pelargonium zonale. 19734763312
[phytochemical and microbiological control of certain plant species used in folk medicine. i. pelargonium zonale (l) ait]. 19725080567
histochemical investigations on the in vivo effects of fluoride on tricarboxylic acid cycle dehydrogenases from pelargonium zonale. ii. 19676033028
a transfer rnaarg gene of pelargonium chloroplasts, but not a 5s rna gene, is efficiently transcribed after injection into xenopus oocyte nuclei.we present the primary structure of a chloroplast trnaargacg gene of the plant, pelargonium zonale, and its faithful expression in xenopus oocyte nuclei. this trnaarg gene is located 250 bp downstream of a 5s rna gene within a cloned 5kb long ribosomal dna segment (fig. 1). the pelargonium trnaarg gene shares 97% and 86% sequence homology with trnaargacg genes of spirodela oligorhiza and euglena gracilis chloroplasts, respectively, and also extensive homology (70%) with the corresponding gene of ...19846209611
direct evidence of plastid dna and mitochondrial dna in sperm cells in relation to biparental inheritance of organelle dna in pelargonium zonale by fluorescence/electron microscopy.the fate of plastid dna and mitochondrial dna was followed during spermatogenesis in thin sections of the pollen grains in pelargonium zonale by epifluorescence microscopy, after staining with 4'-6-diamidino-2-phenylindole (dapi), by the combination of fluorescence and electron microscopic images by color image processor, and by immunogold electron microscopy. dapi-stained thin sections showed that there are two different types of fluorescent spots due to organelle dna in the cytoplasm of genera ...19937925486
the selective increase or decrease of organellar dna in generative cells just after pollen mitosis one controls cytoplasmic inheritance.organellar dna in mature pollen grains of eight angiosperm species (actinidia deliciosa lindl., antirrhinum majus l., arabidopsis thaliana (l.) heynh., medicago sativa l., musa acuminata colla, pelargonium zonale (l.) l'hér, petunia hybrida vilm. and rhododendron mucronatum (blume) g. don, in which the modes of organellar inheritance have been determined genetically, was observed by fluorescence microscopy using technovit 7100 resin sections double-stained with 4',6-diamidino-2-phenylindole (dap ...199910467031
characterization and in vitro translation analysis of pelargonium flower break virus.pelargonium flower break virus (pfbv) is one of the common viruses in the glasshouses of western europe and has been assigned to the genus carmovirus. a spanish isolate obtained from nursery-grown pelargonium zonale plants (pfbv-m) has been characterized. the molecular weight of genomic rna and coat protein of pfbv-m were determined to be 1.36 x 10(6) (corresponding to approximately 4 kb) and 36,000, respectively. only genomic-size rna was encapsidated in pfbv virions; making necessary to purify ...199910486116
empirical investigations into the tunica structuring point of the shoot apex of pelargonium zonale.a tunica structuring point of 3.85 cell units was formally predicted for the shoot apex. this point is decisive for the genesis of the tunica corpus structure as such and for the number of tunica layers in an apex at any given moment. here, a method to determine, in empirical investigations, the theoretical number of tunica layers in real apices is demonstrated. furthermore, results of an empirical investigation into the postulated tunica structuring point are presented and causes for deviations ...200010717323
optical properties and nondestructive estimation of anthocyanin content in plant leaves.absorption and reflectance spectra of maple (acer platanoides), cotoneaster (cotoneaster alaunica), dogwood (cornus alba) and pelargonium (pelargonium zonale) leaves with a wide range of pigment content and composition were studied in visible and near-infrared spectra in order to reveal specific anthocyanin (anth) spectral features in leaves. comparing absorption spectra of anth-containing and anth-free leaves with the same chlorophyll (chl) content, absorption spectra of anth in leaves were der ...200111460535
chloroplast division machinery as revealed by immunofluorescence and electron microscopy.the division of chloroplasts (plastids) is critical for the viability of photosynthetic eukaryotes. previously we reported on the chloroplast division apparatus, which consists of inner and outer double or triple rings (pd rings). chloroplasts are assumed to arise from bacterial endosymbionts, while bacterial division is instigated by a bacterial cytokinesis z-ring protein (ftsz). here we present immunofluorescence and electron-microscopic evidence of chloroplast division via complex machinery i ...200212029466
topical hemostatic effect of a common ornamental plant, the geraniaceae pelargonium zonale.geranium has been traditionally used as a local hemostatic medicine in some andean regions, but this effect has not been tested in controlled experiments. in the present report, the leaves of a geraniaceae (pelargonium zonale) were tested on a bleeding rat model. the bleeding time was 50% shorter in the geranium leaf juice treatment group (18.10 +/- 2.03 min) and 80% shorter in the geranium crushed-leaf group (7.10 +/- 0.88 min) than in the control (nontreatment) group (37.6 +/- 3.04 min), p < 0 ...200312638398
development of cuscuta species on a partially incompatible host: induction of xylem transfer cells.the growth of dodders, cuscuta reflexa and cuscuta japonica, on the partially incompatible host poinsettia ( euphorbia pulcherrima) is studied. poinsettia responds by bark growths to the formation of the dodder haustoria and prevents dodder from obtaining normal growth. the growth instead becomes extremely branched, coral-like, and dodder lacks the ability to form haustoria. after a period of coral-like growth, long shoots sprout, resembling the normal growth. these long shoots mark an ending ph ...200312664277
[biochemical and histochemical study of tumors of the crown gall in pelargonium zonale. ii. phosphatases]. 195313148803
[biochemical study of the crown gall tumors of pelargonium zonale. i. study of the glucides]. 195014771607
[attempts at localization of phosphatases in tissues of pelargonium zonale]. 195114847283
[development of experimental plant carcinomata in pelargonium zonale under the action of vitamin k]. 195114849030
[technique of study of phosphatase distribution during the development of experimental tumors in pelargonium zonale]. 195114936322
comparative phloem mobility of nickel in nonsenescent plants.(63)ni was applied to nonsenescent source leaves and found to be transported to sink tissues in pea (pisum sativum l.) and geranium plants (pelargonium zonale l.). the comparative mobilities (percent tracer transported out of source leaf /% (86)rb transported) for (63)ni in peas was 2.12 and in geranium 0.25. the value for the phloem mobile (86)rb was 1.00. by contrast, the comparative mobility of (45)ca, which is relatively immobile in the phloem, was low (0.05 in peas, 0.00 in geranium). inter ...198616664881
phytophthora tropicalis on hedera helix and epipremnum aureum in polish greenhouses.phytophthora tropicalis was isolated from hedera helix and epipremnum aureum showing discoloration of leaves, necrosis of shoot base, spread upwards and on roots. the species was detected from 7/8 plants of hedera and 3/4 of epipremnum. additionally botrytis cinerea, fusarium avenaceum and rhizoctonia solani were recovered from some of diseased plants. p. tropicalis caused leaf necrosis of 13 plant species and tomato seedlings. the quickest spread of necrosis was observed on leaves of peperomia ...200617390874
light absorption by isolated chloroplasts and leaves: effects of scattering and 'packing'.light absorption was quantified in the following systems: isolated chloroplasts and leaves of spinach (spinacea oleracea l.), a mutant of geranium (pelargonium zonale l.) widely differing in pigment content, and coleus (coleus blumei benth.) at different stages of leaf ontogenesis. for these species and pea (pisum sativum l.), scattering-compensated absorption spectra of chloroplast suspensions are presented. comparison of leaf and chloroplast spectra showed considerable changes in the extent of ...200919672688
biparental inheritance of plastidial and mitochondrial dna and hybrid variegation in pelargonium.plastidial (pt) and mitochondrial (mt) genes usually show maternal inheritance. non-mendelian, biparental inheritance of plastids was first described by baur (z indukt abstamm vererbungslehre 1:330-351, 1909) for crosses between pelargonium cultivars. we have analyzed the inheritance of pt and mtdna by examining the progeny from reciprocal crosses of pelargonium zonale and p. inquinans using nucleotide sequence polymorphisms of selected pt and mt genes. sequence analysis of the progeny revealed ...200919787375
the levels of male gametic mitochondrial dna are highly regulated in angiosperms with regard to mitochondrial inheritance.the mechanisms that regulate mitochondrial inheritance are not yet clear, even though it is 100 years since the first description of non-mendelian genetics. here, we quantified the copy numbers of mitochondrial dna (mtdna) in the gametic cells of angiosperm species. we demonstrate that each egg cell from arabidopsis thaliana, antirrhinum majus, and nicotiana tabacum possesses 59.0, 42.7, and 73.0 copies of mtdna on average, respectively. these values are equivalent to those in arabidopsis mesoph ...201020605854
analysis of the photosynthetic response induced by variation potential in geranium.electrical signals (action and variation potentials) caused by environmental stimuli induce a number of physiological responses in plants including changes in photosynthesis; however, mechanisms of these changes remain unclear. we investigated the influence of the variation potential on photosynthesis in geranium (pelargonium zonale) under different conditions (control, low external co(2) concentration, and actinic light absence). the variation potential caused by lamina burning induced a reduct ...201122020752
production of engineered long-life and male sterile pelargonium plants.pelargonium is one of the most popular garden plants in the world. moreover, it has a considerable economic importance in the ornamental plant market. conventional cross-breeding strategies have generated a range of cultivars with excellent traits. however, gene transfer via agrobacterium tumefaciens could be a helpful tool to further improve pelargonium by enabling the introduction of new genes/traits. we report a simple and reliable protocol for the genetic transformation of pelargonium spp. a ...201222935247
analysis of antifungal and anticancer effects of the extract from pelargonium zonale.the extract from pelargonium zonale stalks exhibits activity against candida albicans and exerts an effect on the hela cell line. the action against c. albicans cells was analysed using light, clsm, sem, and tem microscopes. the observations indicate that the extract influenced fungal cell morphology and cell metabolic activity. the morphological changes include cell wall damage, deformations of cell surfaces, and abnormalities in fungal cell shape and size. cells of c. albicans treated with the ...201424972056
development and life table parameters of phenacoccus madeirensis green (hemiptera: pseudococcidae) on four ornamental plants.the development, reproduction, and life table parameters of the phenacoccus madeirensis green (hemiptera: pseudococcidae) on four ornamental plant species, namely pelargonium zonale (geraniaceae), hibiscus rosa-sinensis, hibicus syriacus (malvaceae), and cestrum nocturnum (solanaceae) were investigated under controlled conditions (25 ± 2°c, 60 ± 10% r.h., and 16 h photophase). life table data were analyzed by using an age-stage two-sex life table. the shortest total immature developmental time o ...201626951150
contributions regarding the leaf histo-anatomy of some pelargonium species.pelargonium l. genra (fam. geraniaceae) includes over 250 species, most of it native to southern africa. nowadays, four perennial species are cultivated in romania as ornamental plants. our aim was to establish the main characteristics and differences that occur between pelargonium zonale, p. hispidum, p. grandiflorum, p. peltatum and p. radens. all in all, the quantitative differences (regarding the leaf-stalk, blade and trichomes) have a taxonomical value for all five species included in the r ...201424502056
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