| xyloglucan oligosaccharide alpha-l-fucosidase activity from growing pea stems and germinating nasturtium seeds. | [14c]fucose-labelled xyloglucan (xg) was synthesized from tamarind seed xg by incubating it with gdp-[14c]fucose plus solubilized pea fucosyltransferase, and [14c]fucose-labelled xg nonasaccharide was prepared from the parent hemicellulose by partial hydrolysis with fungal cellulase. alpha-l-fucosidase activity was readily detected in crude enzyme extracts of growing regions of etiolated pea stems (pisum sativum) and in cotyledons of germinating nasturtium seedlings (tropaeolum majus) using the ... | 1991 | 1367786 |
| substrate subsite recognition of the xyloglucan endo-transglycosylase or xyloglucan-specific endo-(1-->4)-beta-d-glucanase from the cotyledons of germinated nasturtium (tropaeolum majus l.) seeds. | we have investigated the substrate subsite recognition requirement of the xyloglucan endo-transglycosylase/xyloglucan-specific endo-(1-->4)-beta-d-glucanase (nxet) from the cotyledons of nasturtium seedlings. seed xyloglucans are composed almost entirely of the glc4 subunits xxxg, xlxg, xxlg and xllg, where g represents an unsubstituted glucose residue, x a xylose-substituted glucose residue and l a galactosyl-xylose-substituted glucose residue. thus in the xyloglucan sequence shown below, the x ... | 1996 | 8904807 |
| xyloglucan galactosyl- and fucosyltransferase activities from pea epicotyl microsomes. | microsomal membranes from growing tissue of pea (pisum sativum l.) epicotyls were incubated with the substrate udp-[14c]galactose (gal) with or without tamarind seed xyloglucan (xg) as a potential galactosyl acceptor. added tamarind seed xg enhanced incorporation of [14c]gal into high-molecular-weight products (eluted from columns of sepharose cl-6b in the void volume) that were trichloroacetic acid-soluble but insoluble in 67% ethanol. these products were hydrolyzed by cellulase to fragments co ... | 1997 | 9159950 |
| action of a pure xyloglucan endo-transglycosylase (formerly called xyloglucan-specific endo-(1-->4)-beta-d-glucanase) from the cotyledons of germinated nasturtium seeds. | the action on tamarind seed xyloglucan of the pure, xyloglucan-specific endo-(1-->4)-beta-d-glucanase from nasturtium (tropaeolum majus l.) cotyledons has been compared with that of a pure endo-(1-->)-beta-d-glucanase ('cellulase') of fungal origin. the fungal enzyme hydrolysed the polysaccharide almost completely to a mixture of the four xyloglucan oligosaccharides: [formula: see text] exhaustive digestion with the nasturtium enzyme gave the same four oligosaccharides plus large amounts of high ... | 1993 | 8374618 |
| in vitro and in vivo antitumor activity of benzyl isothiocyanate: a natural product from tropaeolum majus. | cultured cells of tropaeolum majus produce significant amounts of benzyl glucosinolate which, through enzymatic hydrolysis, results in the production of benzyl isothiocyanate (bitc). this study reports on the in vitro anticancer properties of bitc against a variety of human and murine tumor cell lines by four independent methods; srb, mtt, cell counting, and clonogenic assays. regardless of the assay used, bitc showed promising cytotoxicity in the low micromolar range (0.86 to 9.4 microm) agains ... | 1995 | 7617765 |
| the amino acid sequences of cytochrome c from four plant sources. | proposed amino acid sequences of cytochrome c from nasturtium (tropaeolum majus l.), box-elder (acer negundo l.), elder (sambucus nigra l.) and parsnip (pastinaca sativa l.) are presented. because of the very limited amounts of cytochrome available from some plant sources, peptides derived from the cytochromes c have been sequenced by the semi-quantitative dansyl-edman technique (gray & hartley, 1963) without supporting quantitative amino acid analyses. because of the qualitative nature of the w ... | 1974 | 4362498 |
| purification and properties of a novel xyloglucan-specific endo-(1----4)-beta-d-glucanase from germinated nasturtium seeds (tropaeolum majus l.). | endo-(1----4)-beta-d-glucanase activity has previously been detected in the cotyledons of germinated nasturtium (tropaeolum majus l.) seeds, and has been linked to the hydrolysis in vivo of storage xyloglucan (amyloid) (edwards, m., dea, i. c. m., bulpin, p. v., and reid, j. s. g. (1985) planta (berl.) 163, 133-140). extracts from the cotyledons of 14-day seedlings are now shown to contain a single endo-glucanase activity, and it has been purified to apparent homogeneity by sequential anion-exch ... | 1986 | 3722207 |
| enzyme-gold cytochemistry of seed xyloglucans using two xyloglucan-specific hydrolases. importance of prior heat-deactivation of the enzymes. | two pure, homogeneous xyloglucan-hydrolyzing enzymes from germinated nasturtium seeds have been used to localize xyloglucans specifically in seed cell walls. the enzymes, a novel endo (1----4)-beta-d-glucanase which shows absolute specificity towards xyloglucans and a beta-d-galactosidase which is capable of removing galactosyl residues from polymeric xyloglucans, were used to stabilize gold sols. the complexes were applied to ultrathin sections of nasturtium (tropaeolum majus l) and tamarind (t ... | 1991 | 1783560 |
| natural isothiocyanates express antimicrobial activity against developing and mature biofilms of pseudomonas aeruginosa. | the antimicrobial properties of natural isothiocyanates (itcs) found in plants such as nasturtium (tropaeolum majus) and horseradish (armoracia rusticana), and the need of new chemotherapeutic options for treatment of infections caused by multidrug-resistant and biofilm-forming gram-negative bacteria such as pseudomonas aeruginosa (pa), led us to evaluate the effects of three major itcs, allylisothiocyanate (aitc), benzylisothiocyanate (bitc), and phenylethyl-isothiocyanate (peitc), and a mixtur ... | 2017 | 28390975 |
| antihypertensive effects of isoquercitrin and extracts from tropaeolum majus l.: evidence for the inhibition of angiotensin converting enzyme. | previous studies have shown that the extracts obtained from tropaeolum majus l. exhibit pronounced diuretic properties. in the present study, we assessed whether the hypotensive and/or antihypertensive mechanism of hydroethanolic extract (hetm), semi-purified fraction (tmlr) obtained from t. majus and the flavonoids isoquercitrin (isq) and kaempferol (kpf) can be mediated by their interaction with angiotensin converting enzyme (ace). | 2010 | 21185932 |
| diuretic and potassium-sparing effect of isoquercitrin-an active flavonoid of tropaeolum majus l. | previous studies have shown that the extracts obtained from tropaeolum majus l. exhibit pronounced diuretic effects supporting the ethnopharmacological use of this plant as diuretic. in the present work, phytochemical investigation, guided by bio-assay in spontaneously hypertensive rats (shr), was carried out in order to identify the compounds responsible for diuretic action. | 2010 | 21163342 |
| effect of the label of oligosaccharide acceptors on the kinetic parameters of nasturtium seed xyloglucan endotransglycosylase (xet). | fluorescently labeled derivatives of a xyloglucan (xg) nonasaccharide glc(4)xyl(3)gal(2) (xllg) were used as glycosyl acceptors in assays of xyloglucan endotransglycosylase (xet) from germinated nasturtium (tropaeolum majus) seeds. we have investigated how the type of the oligosaccharide label influences the kinetic parameters of the reaction. the fluorescent probes used to label xllg were anthranilic acid (aa), 8-aminonaphtalene-1,3,6-trisulfonic acid (ants), fluorescein isothiocyanate (fitc), ... | 2010 | 21146161 |
| the protective role of tropaeolum majus on blood and liver toxicity induced by diethyl maleate in rats. | the protective role of tropaelum majus (t.majus) methyl alcohol extract and vitamin e in the case of toxic effect induced by diethyl maleate was evaluated. forty-two male albino rats were divided into seven groups of six rats each for 15 days. group 1: normal control group. group 2: taken daily oral dose of paraffin oil (0.25ml/100g b.wt rat). group 3: received daily oral dose of vitamin e (100mg/kg b.wt rat). group 4: taken daily oral dose of 10% of the ld50 of t.majus methyl alcohol extract. g ... | 2010 | 20883155 |
| lysophosphatidate acyltransferase in the microsomes from maturing seeds of meadowfoam (limnanthes alba). | lysophosphatidate (lpa) acyltransferase (ec 2.3. 1.51) in the microsomes from the maturing seeds of meadowfoam (limnanthes alba), nasturtium (tropaeolum majus), palm (syagrus cocoides), castor bean (ricinus communis), soybean (glycine max), maize (zea mays), and rapeseed (brassica napus) were tested for their specificities toward 1-oleoyl-lpa or 1-erucoyl-lpa, and oleoyl coenzyme a (coa) or erucoyl coa. all the enzymes could use either of the two acyl acceptors and oleoyl coa, but only the meado ... | 1990 | 16667817 |
| fructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower. | a fructophilic lactic acid bacterium, designated strain f214-1(t), was isolated from a flower of tropaeolum majus in south africa. based on phylogenetic analysis of 16s rrna gene sequences, the strain formed a subcluster with fructobacillus ficulneus and fructobacillus pseudoficulneus and, based on reca gene sequences, the strain formed a subcluster with f. ficulneus. dna-dna hybridization studies showed that strain f214-1(t) was phylogenetically distinct from its closest relatives. acid was pro ... | 2011 | 20495031 |
| polysaccharide microarrays for high-throughput screening of transglycosylase activities in plant extracts. | polysaccharide transglycosylases catalyze disproportionation of polysaccharide molecules by cleaving glycosidic linkages in polysaccharide chains and transferring their cleaved portions to hydroxyl groups at the non-reducing ends of other polysaccharide or oligosaccharide molecules. in plant cell walls, transglycosylases have a potential to catalyze both cross-linking of polysaccharide molecules and grafting of newly arriving polysaccharide molecules into the cell wall structure during cell grow ... | 2010 | 19953317 |
| natriuretic and diuretic effects of tropaeolum majus (tropaeolaceae) in rats. | tropaeolum majus l. (tropaeolaceae), popularly known as "chaguinha", is well recognized in brazilian traditional medicine as diuretic agent, although no scientific data have been published to support this effect. | 2009 | 19429322 |
| purification of xyloglucan endotransglycosylase based on affinity sorption of the active glycosyl-enzyme intermediate complex to cellulose. | xyloglucan endotransglycosylase (xet) catalyzes the cleavage of xyloglucan (xg) molecules by a transglycosylation mechanism involving two steps: (a) endocleavage of the beta-(1,4)-linked polyglucosyl backbone of the xyloglucan molecule with formation of a glycosyl-enzyme intermediate; (b) transfer of the glycosyl residue from the intermediate to the c-4 position of the nonreducing end glucosyl unit of another molecule of xg or an xg-derived oligosaccharide with liberation of the enzyme (z. sulov ... | 1999 | 10419819 |
| functional characterization of xyloglucan glycosynthases from gh7, gh12, and gh16 scaffolds. | glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates, are emerging as useful tools for the synthesis of large, complex polysaccharides [faijes, m.; planas, a. carbohydr. res. 2007, 342, 1581-1594]. guided by wild-type xyloglucanase activity, we have produced and characterized new glycosynthases for the synthesis of xyloglucan oligo- and polysaccharides, based on family gh7, gh12, and gh16 scaffolds. the humicol ... | 2009 | 19419143 |
| analysis of nasturtium tmnxg1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family gh16 xyloglucan endo-transglycosylases and endo-hydrolases. | reorganization and degradation of the wall crosslinking and seed storage polysaccharide xyloglucan by glycoside hydrolase family 16 (gh16) endo-transglycosylases and hydrolases are crucial to the growth of the majority of land plants, affecting processes as diverse as germination, morphogenesis, and fruit ripening. a high-resolution, three-dimensional structure of a nasturtium (tropaeolum majus) endo-xyloglucanase loop mutant, tmnxg1-deltayniig, with an oligosaccharide product bound in the negat ... | 2009 | 19004021 |
| cloning and characterization of an acyl-coa-dependent diacylglycerol acyltransferase 1 (dgat1) gene from tropaeolum majus, and a study of the functional motifs of the dgat protein using site-directed mutagenesis to modify enzyme activity and oil content. | summary: a full-length cdna encoding a putative diacylglycerol acyltransferase 1 (dgat1, ec 2.3.1.20) was obtained from tropaeolum majus (garden nasturtium). the 1557-bp open reading frame of this cdna, designated tmdgat1, encodes a protein of 518 amino acids showing high homology to other plant dgat1s. the tmdgat1 gene was expressed exclusively in developing seeds. expression of recombinant tmdgat1 in the yeast h1246matalpha quadruple mutant (dga1, lro1, are1, are2) restored the capability of t ... | 2008 | 18631243 |
| kinetic analyses of retaining endo-(xylo)glucanases from plant and microbial sources using new chromogenic xylogluco-oligosaccharide aryl glycosides. | a library of phenyl beta-glycosides of xylogluco-oligosaccharides was synthesized via a chemoenzymatic approach to produce new, specific substrates for xyloglucanases. tamarind xyloglucan was completely hydrolyzed to four, variably galactosylated component oligosaccharides based on glc 4 backbones, using a trichoderma endo-glucanase mixture. oligosaccharide complexity could be further reduced by beta-galactosidase treament. subsequent per- o-acetylation, alpha-bromination, phase-transfer glycosy ... | 2008 | 18627132 |
| structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism. | high-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (gh16) endo-xyloglucanases tm-nxg1 and tm-nxg2 from nasturtium (tropaeolum majus) have been solved by x-ray crystallography. key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the gh16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo- ... | 2007 | 17557806 |
| a gene from the cellulose synthase-like c family encodes a beta-1,4 glucan synthase. | despite the central role of xyloglucan (xyg) in plant cell wall structure and function, important details of its biosynthesis are not understood. to identify the gene(s) responsible for synthesizing the beta-1,4 glucan backbone of xyg, we exploited a property of nasturtium (tropaeolum majus) seed development. during the last stages of nasturtium seed maturation, a large amount of xyg is deposited as a reserve polysaccharide. a cdna library was produced from mrna isolated during the deposition of ... | 2007 | 17488821 |
| seed-specific heterologous expression of a nasturtium fae gene in arabidopsis results in a dramatic increase in the proportion of erucic acid. | the fatty acid elongase [often designated fae or beta-(or 3-) ketoacyl-coa synthase] is a condensing enzyme and is the first component of the elongation complex involved in synthesis of erucic acid (22:1) in seeds of garden nasturtium (tropaeolum majus). using a degenerate primers approach, a cdna of a putative embryo fae was obtained showing high homology to known plant elongases. this cdna contains a 1,512-bp open reading frame that encodes a protein of 504 amino acids. a genomic clone of the ... | 2004 | 15333757 |
| microsomal lyso-phosphatidic acid acyltransferase from a brassica oleracea cultivar incorporates erucic acid into the sn-2 position of seed triacylglycerols. | developing seeds from brassica oleracea (l.) var botrytis cv sesam were examined for the ability to biosynthesize and incorporate erucic acid into triacylglycerols (tags). seed embryos at mid-development contained a high concentration of erucic acid in diacylglycerols and tags, and substantial levels were also detected in free fatty acids, acyl-coenzyme a (coa), phosphatidic acid, and phosphatidylcholine. homogenates and microsomal fractions from seeds at mid-development produced [14c]eicosenoyl ... | 1995 | 12228602 |
| ten isoenzymes of xyloglucan endotransglycosylase from plant cell walls select and cleave the donor substrate stochastically. | to map the preferred cleavage sites of xyloglucan endotransglycosylases (xets; ec 2.4.1.207) along the donor substrate chain, we incubated the enzymes with tamarind (tamarindus indica) xyloglucan (donor substrate; approximately 205 kda; 21 microm) plus the nonasaccharide [(3)h]xllgol (gal(2).xyl(3).glc(3). [(3)h]glucitol; acceptor substrate; 0.6 microm). after short incubation times, to minimize multiple cleavages, the size of the (3)h-labelled transglycosylation products (determined by gel-perm ... | 2001 | 11311129 |
| sugar-nucleotide-binding and autoglycosylating polypeptide(s) from nasturtium fruit: biochemical capacities and potential functions. | polypeptide assemblies cross-linked by s-s bonds (molecular mass>200 kda) and single polypeptides folded with internal s-s cross-links (<41 kda) have been detected by sds/page in particulate membranes and soluble extracts of developing cotyledons of nasturtium (tropaeolum majus l.). when first prepared from fruit homogenates, these polypeptides were found to bind reversibly to udp-gal (labelled with [(14)c]gal or [(3)h]uridine), and to co-precipitate specifically with added xyloglucan from solut ... | 2000 | 10769192 |
| the presence of cyp79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates. | a cdna encoding cyp79b1 has been isolated from sinapis alba. cyp79b1 from s. alba shows 54% sequence identity and 73% similarity to sorghum cyp79a1 and 95% sequence identity to the arabidopsis t42902, assigned cyp79b2. the high identity and similarity to sorghum cyp79a1, which catalyses the conversion of tyrosine to p-hydroxyphenylacetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, suggests that cyp79b1 similarly catalyses the conversion of amino acid(s) to aldoxime(s) in the b ... | 1998 | 9862490 |
| a xyloglucan oligosaccharide-active, transglycosylating beta-d-glucosidase from the cotyledons of nasturtium (tropaeolum majus l) seedlings--purification, properties and characterization of a cdna clone. | a beta-d-glucosidase has been purified to apparent homogeneity from the cotyledons of germinated nasturtium (tropaeolum majus l.) seedlings during the mobilization of the xyloglucan stored in the cotyledonary cell walls. the purified protein (mr 76, 000; a glycoprotein; pl > 9.5; apparent ph optimum 4.5; temperature optimum 30 degrees c) catalysed the hydrolysis of p-nitrophenyl-beta-d-glucopyranoside, cello-oligosaccharides, beta-linked glucose disaccharides, and certain xyloglucan oligosacchar ... | 1998 | 9744092 |
| direct observation of cell wall structure in living plant tissues by solid-state c nmr spectroscopy. | solid-state (13)c nuclear magnetic resonance (nmr) spectra of the following intact plant tissues were recorded by the crosspolarization magic-angle spinning technique: celery (apium graveolens l.) collenchyma; carob bean (ceratonia siliqua l.), fenugreek (trigonella foenum-graecum l.), and nasturtium (tropaeolum majus l.) endosperm; and lupin (lupinus polyphyllus lindl.) seed cotyledons. all these tissues had thickened cell walls which allowed them to withstand the centrifugal forces of magic an ... | 1990 | 16667266 |
| expression of heterologous xyloglucan xylosyltransferases in arabidopsis to investigate their role in determining xyloglucan xylosylation substitution patterns. | putative xyg xylosyltransferases from tropaeolum majus (nasturtium) and solanum lycopersicum (tomato) homologous to characterized arabidopsis genes were identified and shown to functionally complement arabidopsis mutants lacking xyloglucan demonstrating they represent xyloglucan xylosyltransferases. xyloglucan is a major hemicellulose in the plant cell wall and is important for the structural organization of the wall. the fine structure of xyloglucan can vary dependent on plant species and tissu ... | 2015 | 25604050 |
| developmental events leading to peltate leaf structure in tropaeolum majus (tropaeolaceae) are associated with expression domain changes of a yabby gene. | peltate leaf architecture has evolved from conventional bifacial leaves many times in flowering plant evolution. characteristics of peltate leaves, such as the differentiation of a cross zone and of a radially symmetric, margin-less petiole, have also been observed in mutants of genes responsible for adaxial-abaxial polarity establishment. this suggests that altered regulation of such genes provided a mechanism for the evolution of peltate leaf structure. here, we show that evolution of leaf pel ... | 2005 | 15791422 |
| molecular characterisation of a xyloglucan oligosaccharide-acting alpha-d-xylosidase from nasturtium (tropaeolum majus l.) cotyledons that resembles plant 'apoplastic' alpha-d-glucosidases. | we report the isolation, sequencing and analysis of the cdna corresponding to an alpha-d-xylosidase involved in the mobilisation of xyloglucan from the cotyledons of germinated nasturtium (tropaeolum majus l.) seeds. the translated open reading frame (2,808 bp including the stop codon), gave a polypeptide of 935 amino acids. it included the sequences of eleven peptides obtained by endo-proteinase digestion of the protein, and a putative hydrophobic signal sequence characteristic of a protein tha ... | 2002 | 11859845 |
| senescence and stomatal aperture as affected by antibiotics in darkness and light. | in leaves of barley (hordeum vulgare), as previously found with oats (avena sativa), a group of six antibiotics that interfere in different ways with the sequence dna --> mrna --> protein all delay senescence in the dark, acting to conserve chlorophyll (chl) and protein and also to open the stomata. among the active compounds is chloramphenicol, which had previously been reported to act only on procaryotes. it is now shown that all these compounds with senescence-delaying action in darkness have ... | 1990 | 16667337 |
| comparative deep transcriptional profiling of four developing oilseeds. | transcriptome analysis based on deep expressed sequence tag (est) sequencing allows quantitative comparisons of gene expression across multiple species. using pyrosequencing, we generated over 7 million ests from four stages of developing seeds of ricinus communis, brassica napus, euonymus alatus and tropaeolum majus, which differ in their storage tissue for oil, their ability to photosynthesize and in the structure and content of their triacylglycerols (tag). the larger number of ests in these ... | 2011 | 21851431 |
| formation of (n-9) and (n-7) cis-monounsaturated fatty acids in seeds of higher plants. | the relative abundance of (n-9) and (n-7) isomers in the monounsaturated fatty acids of seed lipids has been determined for selected plants in order to assess the biosynthetic reactions involved in their formation. δ9 desaturation of stearic acid to (n-9) octadecenoic acid is almost exclusively operative in the formation of monounsaturated fatty acids in the seeds of helianthus annuus, glycine max and brassica napus, cv. quinta and erglu, in which chain elongation of monounsaturated fatty acids ... | 1980 | 24306473 |
| chemosensory basis of behavioural plasticity in response to deterrent plant chemicals in the larva of the small cabbage white butterfly pieris rapae. | behavioural and electrophysiological responsiveness to three chemically different secondary plant substances was studied in larvae of pieris rapae l. (lepidoptera: pieridae). three groups of caterpillars were studied that during their larval development were exposed to different rearing diets: an artificial diet or one of two host-plants, cabbage, brassica oleracea, or nasturtium, tropaeolum majus. in dual-choice leaf disc assays, caterpillars reared on cabbage were strongly deterred by the phen ... | 2009 | 19414011 |
| predatory behavior of polistes dominulus wasps in response to cardenolides and glucosinolates in pieris napi caterpillars. | to examine how plant allelochemicals in prey affect foraging choices made by generalist predator paper wasps, polistes dominulus (vespidae), we compared predation on pieris napi (pieridae) caterpillars reared on host plants with different allelochemicals. in naturalistic behavioral choice experiments, free-flying wasps chose between living pierids reared on cabbage (brassica oleracea), which lacks deterrent allelochemicals, or alternate host plants with potentially deterrent allelochemicals. the ... | 2007 | 17453324 |
| group iii-a xth genes of arabidopsis encode predominant xyloglucan endohydrolases that are dispensable for normal growth. | the molecular basis of primary wall extension endures as one of the central enigmas in plant cell morphogenesis. classical cell wall models suggest that xyloglucan endo-transglycosylase activity is the primary catalyst (together with expansins) of controlled cell wall loosening through the transient cleavage and religation of xyloglucan-cellulose cross links. the genome of arabidopsis (arabidopsis thaliana) contains 33 phylogenetically diverse xyloglucan endo-transglycosylase/hydrolase (xth) gen ... | 2013 | 23104861 |
| rna-seq analysis of developing nasturtium seeds (tropaeolum majus): identification and characterization of an additional galactosyltransferase involved in xyloglucan biosynthesis. | a deep-sequencing approach was pursued utilizing 454 and illumina sequencing methods to discover new genes involved in xyloglucan biosynthesis. cdna sequences were generated from developing nasturtium (tropaeolum majus) seeds, which produce large amounts of non-fucosylated xyloglucan as a seed storage polymer. in addition to known xyloglucan biosynthetic genes, a previously uncharacterized putative xyloglucan galactosyltransferase was identified. analysis of an arabidopsis thaliana mutant line d ... | 2012 | 22474179 |
| a real-time fluorogenic assay for the visualization of glycoside hydrolase activity in planta. | there currently exists a diverse array of molecular probes for the in situ localization of polysaccharides, nucleic acids, and proteins in plant cells, including reporter enzyme strategies (e.g. protein-glucuronidase fusions). in contrast, however, there is a paucity of methods for the direct analysis of endogenous glycoside hydrolases and transglycosidases responsible for cell wall remodeling. to exemplify the potential of fluorogenic resorufin glycosides to address this issue, a resorufin beta ... | 2009 | 19783642 |