| foreign dna sequences are received by a wild-type strain of aspergillus niger after co-culture with transgenic higher plants. | different transgenic plants of brassica napus, brassica nigra, datura innoxia and vicia narbonensis expressing the hph gene under the control of the 35s promoter were co-cultivated with mycelial material of aspergillus niger in microcosms under sterile conditions. a significantly higher number of hygromycin b-resistant colonies of re-isolated fungi was obtained if compared with co-cultures with non-transgenic plants. the hph gene and other foreign sequences could be detected in some of the resis ... | 1994 | 7750149 |
| a chimeric gene encoding the methionine-rich 2s albumin of the brazil nut (bertholletia excelsa h.b.k.) is stably expressed and inherited in transgenic grain legumes. | the coding region of the 2s albumin gene of brazil nut (bertholletia excelsa h.b.k.) was completely synthesized, placed under control of the cauliflower mosaic virus (camv) 35s promoter and inserted into the binary vector plasmid pgsgluc1, thus giving rise to pgsgluc1-2s. this was used for transformation of tobacco (nicotiana tabacum l. cv. petit havanna) and of the grain legume vicia narbonensis l., mediated by the supervirulent agrobacterium tumefaciens strain eha 101. putative transformants w ... | 1994 | 8159174 |
| transformation of vicia narbonensis via agrobacterium-mediated gene transfer. | shoot tips and epicotyl-segments of vicia narbonensis were co-cultivated with agrobacterium tumefaciens strain c58c1 pgv 3850 hpt, carrying a plasmid coding for hygromycin-phosphotransferase. on callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. after transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. th ... | 1991 | 24220706 |
| isolation, purification, crystallization and preliminary crystallographic studies of a chitinase from crocus vernus. | a chitinase has been isolated and purified from crocus vernus corms. n-terminal amino-acid sequence analysis of the approximately 30 kda protein showed 33% identity to narbonin, a seed protein from vicia narbonensis l. the c. vernus chitinase was crystallized by the hanging-drop vapour-diffusion method using peg 8000 as the main precipitant. the crystal belonged to the monoclinic space group c2, with unit-cell parameters a=172.3, b=37.1, c=126.4 å, β=127° and two molecules per asymmetric unit. d ... | 2011 | 21393839 |
| crystallization of seed globulins from legumes. | seeds contain large quantities of proteins and are therefore main food sources. in the last century, protein extracts of legume seeds were dialysed against distilled water and in some cases small crystals of pure protein appeared. however, those crystals were generally of poor quality with respect to x-ray diffraction. recently, the crystallization of some of them was improved and the structures of two 7s globulins, phaseolin from phaseolus vulgaris and canavalin from canavalia ensiformis, have ... | 1994 | 15299427 |
| anti-methicillin-resistant staphylococcus aureus (mrsa) activity of rubiaceae, fabaceae and poaceae plants: a search for new sources of useful alternative antibacterials against mrsa infections. | in this study, we evaluated the effects of the extracts of the leaves of species from the rubiaceae (galium aparine l. and asperula arvensis l.), fabaceae (lathyrus aphaca l. and vicia narbonensis l.) and poaceae (digitaria sanguinalis (l.) scop. and hordeum murinum l.) plant families on a wide and extensive panel of isolated methicillin-resistant staphylococcus aureus strains (mrsa). the effects of the methanolic leaf extracts of rubiaceae, fabaceae and poaceae plants on mrsa were evaluated by ... | 2016 | 27585260 |
| seed-specific expression of a bacterial phosphoenolpyruvate carboxylase in vicia narbonensis increases protein content and improves carbon economy. | an ambitious aim in plant breeding and biotechnology is to increase the protein content of crop seeds used for food and feed. using an approach to manipulate assimilate partitioning, we succeeded in elevating the protein content in legume seeds up to 50%. transgenic bean plants were generated which express a corynebacterium glutamicum phosphoenolpyruvate carboxylase (pepc) in a seed-specific manner. the bacterial enzyme was not feedback inhibited by malate. transgenic seeds showed a higher [14c] ... | 2004 | 17147612 |
| sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of vicia narbonensis l. | in order to change the sugar status during seed development a yeast-derived invertase gene was expressed in cotyledons of vicia narbonensis. as a result, sucrose decreased whereas hexoses accumulated. we analysed cell structure and cellular differentiation in cotyledons expressing the yeast-invertase. transgenic cells contained large and long-persisting vacuoles apparently serving as storage compartments for hexoses and clusters of storage-protein aggregates. in the wild-type, large vacuoles did ... | 2000 | 10987550 |
| expression of a yeast-derived invertase in developing cotyledons of vicia narbonensis alters the carbohydrate state and affects storage functions. | in plants the carbohydrate state provides signals to adjust metabolism to specific physiological conditions. storage-active sink organs like seeds often contain high levels of sucrose. in order to change the sugar status during seed development a yeast-derived invertase gene was expressed in vicia narbonensis under control of the leguminb4 promoter. a signal sequence targeted the invertase to the apoplast in maturing embryos. in the cotyledons, sucrose was decreased whereas hexoses strongly accu ... | 1998 | 9839463 |
| narbonin, a novel 2s protein from vicia narbonensis l. seeds: cdna, gene structure and developmentally regulated formation. | cdna and genomic clones encoding narbonin, a 2s globulin from the seed of narbon bean (vicia narbonensis l.), were obtained using the polymerase chain reaction (pcr) and sequenced. the full-length cdna as well as genomic clones contain a single open reading frame (orf) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (m(r) ca. 33 100) and an initiation methionine. the deduced amino acid sequence lacks a transient n-terminal signal peptide. the geno ... | 1995 | 7787188 |
| subcellular localization of the 2s globulin narbonin in seeds of vicia narbonensis. | narbonin is a 2s protein from the globulin fraction of narbon bean (vicia narbonensis l.) cotyledons. its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. therefore, it was expected to be present in protein bodies of the storage tissue cells. comparison of the cdna-derived amino acid sequence with a directly determined partial n-terminal sequence revealed that the primary translation product o ... | 1997 | 9299790 |
| genetic engineering for high methionine grain legumes. | methionine (met) is the primary limiting essential amino acid in grain legumes. the imbalance in amino acid composition restricts their biological value (bv) to 55 to 75% of that of animal protein. so far improvement of the bv could not be achieved by conventional breeding. therefore, genetic engineering was employed by several laboratories to resolve the problem. three strategies have been followed. a) engineering for increased free met levels; b) engineering of endogenous storage proteins with ... | 1998 | 9739551 |
| comparative analysis of the chromosomal and genomic organization of ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms. | we have investigated the physical distribution of the reverse transcriptase genes of ty1-copia-like retrotransposable elements from 12 plant species belonging to different subdivisions by hybridization in situ on chromosome preparations. ty1-copia-like elements showed different and non-random hybridization patterns. a dispersed distribution throughout most of the chromosomes with reduced hybridization at some regions or with some weak clustering at other regions was found in allium cepa, beta vu ... | 1997 | 9037155 |