| purification and properties of blood-group-specific lectins from vicia cracca. | the lectins from the seeds of vicia cracca react specifically with human blood group a erythrocytes. they were purified by affinity chromatography on an adsorbent containing matrix-bound n-acyl-d-galactosamine. by a continuous ph gradient the lectins could be separated into two fractions each of which was shown to consist of several agglutinating species. the behaviour of both fractions in affinity chromatography was paralleled by the ph dependence of the interaction with the hapten sugar n-acet ... | 1977 | 14000 |
| inhibition of protein synthesis in vitro by a lectin from momordica charantia and by other haemagglutinins. | protein synthesis by a rabbit reticulocyte lysate is inhibited by the haemagglutinating lectins from momordica charantia and crotalaria juncea seeds and from the roe of rutilus rutilus, and by a commercial preparation of the mitogenic lectin from phytolacca americana. the haemagglutinins from the seeds of ricinus communis and of vicia cracca acquired inhibitory activity after their reduction with 2-mercaptoethanol. | 1979 | 508306 |
| studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels. | a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ... | 1978 | 563738 |
| lectin-binding ability of immunoglobulin e and its participation in triggering of mast cells. | eleven purified lectins were compared in their ige-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. a significantly positive relationship could be proved between their ige-precipitating activity and degranulation of ige-sensitized rat mast cells. a similar and often significant relation was observed in skin reactions and histamine released from human leukocytes. soybean lectin reacted with concanavalin a ... | 1985 | 2408980 |
| studies with lectins on the surface carbohydrate structures of mycoplasma membranes. | the surface carbohydrate structures on the cell membranes of various mycoplasma species have been investigated by using lectins, which are sugar-specific proteins. carbohydrate structures presumably bound to glycolipids, with both galactose and glucose units, were found to be exposed on the surface of mycoplasma pneumoniae and its temperature-sensitive mutants, m. mycoides var. mycoides and capri, m. pulmonis, m. gallinarum, and m. gallisepticum. lipid-bound glucose was found on m. neurolyticum. ... | 1974 | 4138526 |
| studies on blood group substances. i. purification of active material from hog gastric mucin by specific precipitation with vicia cracca phytohemagglutinin. | | 1968 | 5660100 |
| antigenic similarities both inside and outside the carbohydrate-binding sites of two-chain and one-chain leguminous lectins. | antibodies were made against the two-chain lectin lath-o from the seeds of lathyrus odoratus as well as its isolated light (alpha) and heavy (beta) chains. these antibodies were used to antigenically compare the lath-o with other two-chain lectins like lath-s, lentil and vicia cracca glc specific and with one-chain lectins like con a, pha, peanut and soybean. sensitive elisa tests showed that there was a distinct antigenic cross-reaction between the one-chain and two-chain lectins both by using ... | 1984 | 6424408 |
| purification and characterization of a mannose/glucose-specific lectin from vicia cracca. | the seeds of vicia cracca are known to contain two lectins: an n-acetylgalactosamine-binding lectin, which reacts specifically with human erythrocytes of blood group a, and a mannose/glucose-binding lectin unspecific towards human blood groups. the second lectin is the object of the present work. purification was achieved by affinity chromatography on sephadex g-100. the native lectin has a molecular weight of 44,000. it is composed of two small (mr 5,700) and two large subunits (mr 17,500). bin ... | 1982 | 7060560 |
| blood-group a and b determinants are located in different polyglycosyl peptides isolated from human erythrocytes of blood-group ab. | the distribution of blood-group a and b determinants was studied by isolating blood-group abh-active polyglycosyl peptides from delipidated human blood-group ab erythrocyte membranes after extensive digestion with pronase followed by chromatography on bandeiraea simplicifolia i (bsi) lectin coupled to sepharose. 20% of the polyglycosyl peptides were bound to bsi lectin. the glycopeptides bound were further fractionated using the blood-group-a-specific lectin from vicia cracca (vc). approximately ... | 1981 | 7202409 |
| the structure of the lentil (lens culinaris) lectin. amino acid sequence determination and prediction of the secondary structure. | the subunit structure and complete amino acid sequence of the lectin extracted from lens culinaris (lcl) seeds was determined. in previous studies, the primary structure of the alpha-chain (mr = 5,710) was shown to be homologous to the alpha-chain of the lectin from pisum sativum, the vicia cracca glucose-specific lectin, and a region in the middle of the concanavalin a sequence (residues 70-121). the complete amino acid sequence of the beta-chain (mr = 17,572) has been determined from 11 trypti ... | 1981 | 7240155 |
| isolation and characterization of the blood group a-specific lectin from vicia cracca. | we have isolated the blood group a-specific lectin from vicia cracca by affinity chromatography on immobilized porcine blood group a/h substance. a molecular weight of 100 000 was obtained by gel filtration and analytical ultracentrifugation. the subunit size when determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was 29 000. the lectin contains no half-cystine or methionine. it is a glycoprotein apparently with one oligosaccharide per subunit. the oligosa ... | 1980 | 7378459 |
| identification of blood group a-active glycoproteins in the human erythrocyte membrane. | normal human erythrocytes of blood groups a1, a2, b and o, and en (a-) erythrocytes lacking glycophorin a, but with a1b-activity, were surface-labeled with tritiated sodium borohydride after oxidation of terminal galactosyl and n-acetylgalactosaminyl residues with galactose oxidase. a1 cells were also labeled by lactoperoxidase catalyzed iodination. after solubilization in triton x-100, the blood group a-active glycoconjugates were isolated using the a-specific lectin from vicia cracca coupled t ... | 1980 | 7378460 |
| expression of blood group a antigens in human bone marrow cells. | we have studied the appearance of blood group a-activity during hematopoiesis in human bone marrow cells by the use of the blood group a-specific lectin from vicia cracca. cells that bound the lectin were identified using antiserum against the lectin followed by rosetting with protein a-containing staphylococcus aureus cells. only cells of the erythroid lineage from blood group a individuals formed staphylococcal rosettes. a-activity occurred in basophilic normoblasts and later stages of erythro ... | 1981 | 7448406 |
| lectins as markers for blood grouping. | lectins are unique proteins of varying biological importance. they are characterized by specific binding to carbohydrate residues, whether monosaccharides, disaccharides or polysaccharides. the sugar heads on the surface of the erythrocyte specify the different blood groups. lectins, as an antigenic determinant of blood group, have come to be an important tool in the identification of different blood groups. a handful of lectins may be considered excellent reagents for anti-a, anti-b, anti-n etc ... | 2002 | 12503049 |
| reproduction of pratylenchus penetrans on 24 common weeds in potato fields in québec. | twenty-four weeds commonly found in commercial potato fields in quebec were evaluated for their host suitability to the root-lesion nematode, pratylenchus penetrans, under greenhouse conditions. brown mustard (brassica juncea) and rye (secale cereale) were included as susceptible controls and forage pearl millet hyb. cfpm 101 (pennisetum glaucum) as a poor host. pratylenchus penetrans multiplied well on 22 of the 24 weed species tested (pf/pi >/= rye or brown mustard). cirsium arvense, leucanthe ... | 2007 | 19259506 |
| transcriptomic analysis of rhizobium leguminosarum biovar viciae in symbiosis with host plants pisum sativum and vicia cracca. | rhizobium leguminosarum bv. viciae forms nitrogen-fixing nodules on several legumes, including pea (pisum sativum) and vetch (vicia cracca), and has been widely used as a model to study nodule biochemistry. to understand the complex biochemical and developmental changes undergone by r. leguminosarum bv. viciae during bacteroid development, microarray experiments were first performed with cultured bacteria grown on a variety of carbon substrates (glucose, pyruvate, succinate, inositol, acetate, a ... | 2009 | 19376875 |
| [the assessment of no adverse effect doses for plant populations chronically exposed to radionuclides of uranium and thorium decay series]. | dose rates cause no adverse effects on natural populations of pinus sylvestris l. and vicia cracca l. inhabiting territories contaminated by uranium mill tailings and radium production wastes (vodny settlement, komi republic) were determined. a significant increase in embryonic lethal mutation frequency in v. cracca legumes and decrease in seedlings survival rate as compared with control values were registered at dose rate equal to 1.67 mgy/day, that is 280 times higher than the one calculated f ... | 2010 | 20968049 |
| [flavonoids of leguminous plants: research on the flowers of vicia cracca]. | | 2016 | 4967105 |
| choice of hydrogen uptake (hup) status in legume-rhizobia symbioses. | the h(2) is an obligate by-product of n-fixation. recycling of h(2) through uptake hydrogenase (hup) inside the root nodules of leguminous plants is often considered an advantage for plants. however, many of the rhizobium-legume symbioses found in nature, especially those used in agriculture are shown to be hup(-), with the plants releasing h(2) produced by nitrogenase activity from root nodules into the surrounding rhizosphere. recent studies have suggested that, h(2) induces plant-growth-promo ... | 2012 | 23139886 |
| genomic and phenotypic characterization of rhizobium gallicum phage vb_rgls_p106b. | the phage p106b (vb_rgls_p106b) is a siphoviridae phage with a narrow spectrum of infectivity, which has been isolated from soils with a history of pea cultivation. the trapping host of p106b is an indigenous strain of rhizobium gallicum (so14b-4) isolated from soils associated with vicia cracca. phenotypic characterization of the phage revealed that p106b has an approximate burst size of 21 p.f.u. per infected cell with 60 min and 100 min eclipse and latent periods, respectively. phage p106b wa ... | 2015 | 25627439 |
| symbiont abundance is more important than pre-infection partner choice in a rhizobium - legume mutualism. | it is known that the genetic diversity of conspecific rhizobia present in root nodules differs greatly among populations of a legume species, which has led to the suggestion that both dispersal limitation and the local environment affect rhizobial genotypic composition. however, it remains unclear whether rhizobial genotypes residing in root nodules are representative of the entire population of compatible symbiotic rhizobia. since symbiotic preferences differ among legume populations, the genet ... | 2016 | 27269381 |
| population structure of root nodulating rhizobium leguminosarum in vicia cracca populations at local to regional geographic scales. | the genetic diversity and population structure of about 350 rhizobium leguminosarum biovar viciae isolates from vicia cracca were analysed. a hierarchical sampling design was used covering three regions, one region in belgium and two in france, in which multiple local v. cracca populations were sampled. rhizobium isolates were genotyped using rapd and by sequencing two chromosomal housekeeping genes (glnii and reca) and one plasmid-borne gene (nodc). twenty-six nodc types and sixty-seven chromos ... | 2014 | 25199986 |
| content of zinc and copper in selected plants growing along a motorway. | in 2011 a study was carried out analyzing the effects of road traffic on bioaccumulation of zinc and copper in selected species of dicotyledonous plants growing on adjacent grasslands. to do the research the plants were sampled from the 9-km-long siedlce bypass, a part of the international route e-30. they were collected during the flowering stage, at following distances from the road: 1, 5, 10, 15 m. the content of zinc and copper was determined with the aas method, with dry mineralisation done ... | 2015 | 26364030 |
| allelopathic effects of medicago sativa l. and vicia cracca l. leaf and root extracts on weeds. | in this study, the allelopathic potential of different concentration (5, 25 and 50%) of m. sativa and v. cracca leaf and root extracts were evaluated on germination and radicle length of four weed species (amaranthus retroflexus l., lolium perenne l., ipomoea hederacea l. and portulaca oleracea l.) in laboratory condition. as a result, germination and radicle length of all species were reduced by the extract from m. sativa and v. cracca leaf and root at different percentage. increasing the water ... | 2007 | 19086510 |
| potential for phytoextraction of pcbs from contaminated soils using weeds. | a comprehensive investigation of the potential of twenty-seven different species of weeds to phytoextract polychlorinated biphenyls (pcbs) from contaminated soil was conducted at two field sites (etobicoke and lindsay) in southern ontario, canada. soil concentrations were 31 microg/g and 4.7 microg/g at each site respectively. all species accumulated pcbs in their root and shoot tissues. mean shoot concentrations at the two sites ranged from 0.42 microg/g for chenopodium album to 35 microg/g for ... | 2010 | 20483449 |
| growth onset, senescence, and reproductive development of meadow species in mesocosms exposed to elevated o3 and co2. | we studied the effects of elevated o3 (40-50 ppb) and co2 (+100 ppm) alone and in combination on the growth onset, relative chlorophyll meter values, and reproductive development of meadow species grown in ground-planted mesocosms using open-top chambers. the 3-year study was conducted in the summers of 2002-2004. elevated o3 decreased the early season coverage of plant communities and delayed the flowering of campanula rotundifolia and vicia cracca. the relative chlorophyll meter values of frag ... | 2007 | 16815612 |
| influences of elevated ozone and carbon dioxide in growth responses of lowland hay meadow mesocosms. | we studied the effects of relatively low levels of o(3) (40-50 ppb) and co(2)-enrichment (+100 ppm) on a northern european lowland hay meadow during the summers 2002-2004 using open-top chambers (otcs) and ground-planted mesocosms. ozone reduced the aboveground biomass of the community (up to 40%), and four out of seven species (campanula rotundifolia, fragaria vesca, trifolium medium, vicia cracca) showed either significant growth reduction and/or visible injuries under elevated o(3). however, ... | 2006 | 16513235 |
| studies on lectins. xliv. the ph dependence of lectin interactions with sugars as determined by affinity electrophoresis. | the ph dependence of association constants of the lectin-sugar complexes was determined by means of affinity electrophoresis. all the lectins studied (from the seeds of dolichos biflorus, glycine soja, lens esculenta and vicia cracca and of the fruiting body of marasmius oreades) were characterized by a similar course of ph dependence of the association constants, with the maximum values at ph 7--9. for concanavalin a and the l-fucose binding ulex europaeus lectin only the association constants ... | 1979 | 33723 |