| multiple specificities of mammalian blood group substances comparatively studied with human isoagglutinins and fractionated anti-h lectins. | purified blood group-active substances derived from different pig, horse, baboon, rhesus monkey and human tissues were quantitatively studied for their haemagglutination inhibiting potency with: (1) human igm anti-a and anti-b; (2) human anti-lea and anti-leb; (3) ulex europaeus extracts separated into lectin fractions with respective l-fucose-inhibitable ('anti-hf') and chitobiose-cellobiose-inhibitable ('anti-hc') combining sites. irrespective of species origin, a and b blood group activity pe ... | 1975 | 49294 |
| the h specificities of butanol extracts of human erythrocytes. | a previous report of other researchers that aqueous phase fractions of erythrocyte stromata of secretors contained more abh blood group activity than similar fractions of non-secretors was confirmed. the recovery of h activity in the butanol phase extracts of both secretors and non-secretors, which had not been seen previously, is reported here for the first time. three h specificities were present when the aqueous and butanol phases of group o secretor stromata were examined in inhibition of ag ... | 1976 | 64017 |
| the lewis antigens and secretor status. | the coincidence of the presence of abh active substances in the saliva of persons with those individuals who possess either lewis-d or lewis-b antigens on epithelial cells of their stomach tissues is examined in terms of the three-dimensional conformations of the oligosaccharide structures which form the abh and lewis antigenic determinants. the h activity in saliva is normally established by examining the inhibition of the agglutination of human h red cells, which possess h (type 2) determints ... | 1979 | 95124 |
| the fucosyl specific lectins of ulex europaeus and sarothamnus scoparius. biochemical characteristics and binding properties to human b-lymphocytes. | from the seeds of the gorse, ulex europaeus and of the broom, sarothamnus scoparius l-fucosyl-specific lectins were isolated by affinity chromatography on l-fucosyl-epoxy-sepharose. the lectins showed similarities in their molecular weights, amino acid composition, carbohydrate content and in the finger-prints of their tryptic peptides. the fluorescein-labeled lectins of both seeds attached especially to the plasma membranes of human b-lymphocytes. | 1978 | 310317 |
| binding of human liver hydrolases by immobilized lectins. | the binding of 22 human liver hydrolase activities by immobilized lectins of six different carbohydrate specificities, namely alpha-d-mannose (glucose), d-n-acetylglucosamine, d-n-acetylgalactosamine, l-fucose, alpha-d-galactose and beta-d-galactose, were examined. differences in binding among these enzymes and within specific enzymes were observed. for example, the neutral forms of alpha-mannosidase and beta-xylosidase were bound by the ulex europaeus lectin i (specific for l-fucose), whereas t ... | 1979 | 426766 |
| activation of tumoricidal properties in mouse macrophages by lymphokines encapsulated in liposomes. | cell-free culture supernatants rich in macrophage-activating factor (maf) activity obtained from mitogen-stimulated f344 rat lymphocytes have been encapsulated within liposomes of differing size and lipid composition and their ability to render normal mouse macrophages cytotoxic for tumor cells in vitro has been compared with that of unencapsulated (free) maf added to the extracellular medium. normal macrophages from c57bl/6, c3h/hen, and c57bl/6 x c3h f1 mice treated with liposome-encapsulated ... | 1979 | 427777 |
| properties of ulex europaeus ii lectin isolated by affinity chromatography. | a lectin was isolated from ulex europaeus seeds by affinity chromatography on affinity adsorbent prepared by copolymerization of acrylamide, n,n'-methylene bisacrylamide and maleylated hog stomach peptone. the lectin is homogeneous as judged by ultracentrifugation (s20,w = 6.4 s), electrophoretic and gel chromatography criteria; it contains 4.2% neutral sugar and 1.4% glucosamine. its molecular weight is approx. 110,000 and the molecule consists of two noncovalently linked protomers which are fo ... | 1979 | 454654 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| a versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations. | a procedure for cell fractionation using lectin-affinity chromatography is described. it consists of a single affinity adsorbent, hog gastric mucin blood group a+h substance covalently coupled to sephadex or sepharose, to which lectins of various specificities can bind. the complex formed, lectin in equilibrium hog a+h substance-sephadex, then serves as an affinity probe for isolating and fractionating cells. the lectins from ulex europaeus, lotus tetragonolobus, helix pomatia, dolichos biflorus ... | 1979 | 573269 |
| localization of carbohydrate components in rat colon with fluoresceinated lectins. | cryostat sections of rat descending colon were studied by fluorescence microscopy after exposure to conjugates of fluorescein isothicoyanate with lectins from glycine max (soybean), triticum vulgaris (wheat germ), ricinus communis (castor bean), ulex europaeus, (gorse), dolichos biflorus (horse gram) and canavalia ensiformis (concanavalin a) (jack bean). no two lectins showed identical patterns of fluorescence. fitc-conjugates of soybean and d. biflorus lectins reacted strongly with the mucus pr ... | 1978 | 670681 |
| cytokeratin expression in corneal endothelium in the iridocorneal endothelial syndrome. | the immunocytologic characteristics of two formalin-fixed, paraffin-embedded corneas from patients with the iridocorneal endothelial (ice) syndrome and unaffected control corneas were studied. binding of polyclonal antisera to factor viii, s-100 protein, involucrin, neuron specific enolase (nse), and the lectins peanut agglutinin and ulex europaeus agglutinin-1 was performed using the standard peroxidase-anti-peroxidase method. we detected reactive patterns of monoclonal antibodies to cytokerati ... | 1992 | 1281463 |
| characterization of heterotypic adherence between transformed human lymphoblastic cells and marrow stromal cells: vcam-1 is a ligand for one of the leukemia cell adhesion proteins. | marrow stromal cells are important in normal myelopoiesis and support growth of leukemia/lymphoma (ll) cells in vitro. we have previously described the heterotypic adherence of a human b-lymphoblastic cell line (utmb-460) to marrow stromal cells (msc). we have extended these observations to a human t-lymphoblastic cell line (cem) and characterized the heterotypic adherence of b- and t-lymphoblastic cell lines to human msc. electron microscopy demonstrated utmb-460 cells were in very close apposi ... | 1992 | 1283595 |
| use of lectins for characterization and purification of human bone marrow cells that express cd34. | the binding of lectins to nucleated cells from human bone marrow was studied in a search for markers that can be used to subdivide further immature hemopoietic cells that are characterized by their expression of cd34. low-density bone marrow cells were indirectly labeled with biotinylated lectins and streptavidin-r-phycoerythrin (sa-rpe) together with fitc-labeled monoclonal anti-cd34. four-parameter flow cytometric analysis was then performed and list mode data analyzed. of the 21 lectins teste ... | 1992 | 1285403 |
| lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (shr). | some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater blood vessels of control (wky) and hypertensive rats (shr). the lectins tested recognized the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-l-fucosyl (ulex europaeus agglutinin, uea-1), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, wga), n-glycolyl-neuraminic acid (limax flavus agglutinin, lfa), and n-acetyl-d-galactosaminyl (helix ... | 1992 | 1297933 |
| bandeiraea simplicifolia lectin i and vicia villosa agglutinin bind specifically to the vomeronasal axons in the accessory olfactory bulb of the rat. | the binding of 21 lectins to the accessory olfactory bulb (aob) of the rat was examined by histochemistry. two lectins [bandeiraea simplicifolia lectin i (bsl-i) and vicia villosa agglutinin (vva)] bound specifically to the vomeronasal (vn) axons in the aob. seven lectins (datura stramonium lectin, erythrina cristagalli lectin, lycoperisicon esculentum lectin, ricinus communis agglutinin i, soybean agglutinin, solanum tuberosum lectin, and ulex europaeus agglutinin) bound to both vn axons in aob ... | 1992 | 1314354 |
| alterations in protein expression and complement resistance of pathogenic naegleria amoebae. | highly pathogenic strains of naegleria fowleri activate the alternative complement pathway but are resistant to lysis. in contrast, weakly pathogenic and nonpathogenic naegleria spp. activate the complement pathway and are readily lysed. the present study was undertaken to determine whether surface components on amoebae accounted for resistance to complement lysis. enzymatic removal of surface components from highly pathogenic n. fowleri with phosphatidylinositol-specific phospholipase c or with ... | 1992 | 1319405 |
| isolation, cultivation, and partial characterization of microvascular endothelium derived from human lung. | primary cultures of peripheral lung lobes were grown in a highly supplemented medium. human lung endothelial cells (hle) were isolated from the mixed population by facs. the cells proliferated rapidly and were serially cultivated for at least 16 passages. both early and late passage cells were positive for the standard endothelial markers. factor viii related-antigen (factor viii r-ag), angiotensin-converting enzyme, acetylated low-density lipoprotein labeled with 1,1'-dioctadecyl-1,3,3,3',3'-te ... | 1992 | 1333246 |
| developmental changes of fucosylated glycoconjugates in rabbit dorsal root ganglia. | developmental changes of the fucosylated glycoconjugates in the dorsal root ganglia (drg) of the rabbit were investigated histochemically using anti-fucosyl gm1 antibody and ulex europaeus agglutinin 1 (uea-1) lectin. neither anti-fucosyl gm1 antibody nor uea-1 lectin bound to the neural tubes or to the neural crest on embryonic day 14 (e14). anti-fucosyl gm1 antibody binds diffusely to the drg of e25. large neurons unreactive with anti-fucosyl gm1 antibody appeared at 1 month and increased with ... | 1992 | 1336587 |
| distribution of cellular carbohydrate moieties in human dysontogenetic brain tumors, especially in craniopharyngioma and epidermoid/dermoid. | cellular carbohydrate moieties of 65 human dysonotogenetic brain tumors (craniopharyngioma, epidermoid/dermoid, rathke cleft cyst, germinoma and non-germinomatous germ-cell tumors) and 60 common brain tumors (glioma, meningioma, neurinoma and pituitary adenoma) were investigated histochemically using sections from ulex europaeus (uea-1), dolichos biflorus (dba), peanut (pna) and soybean (sba), and with anti-blood group a and lewisy (leyy) antibodies. in craniopharyngiomas and epidermoid/dermoids ... | 1992 | 1337421 |
| dendrocytes in verruga peruana and bacillary angiomatosis. | verruga peruana and bacillary angiomatosis are two cutaneous diseases characterized by angiomatous growths linked to the presence of rickettsia-like organisms. these lesions are currently considered to be endothelial hyperplasias and to share many features. by immunohistochemistry and computerized image analysis, we studied the presence of factor-xiiia-positive dendrocytes in these lesions and compared our data with similar research in capillary angiomas and normal skin. other cell lines were st ... | 1992 | 1348435 |
| isolation and culture of synovial microvascular endothelial cells. characterization and assessment of adhesion molecule expression. | in vitro studies investigating the role of the synovial endothelium in the pathogenesis of rheumatoid arthritis (ra) have, until recently, been performed using cultured endothelial cells of nonsynovial macrovascular origin. in an attempt to more correctly model in vivo conditions, a method for the isolation and culture of synovial microvascular endothelial cells (smec) has been developed. | 1992 | 1373621 |
| localization of prostatic glycoconjugates by the lectin-gold method. | the glycoconjugates of the lateral prostate were examined ultrastructurally by lectin-gold histochemistry in combination with a low-temperature embedding technique using lowicryl k4m. the binding patterns of concanavalin a, wheat germ agglutinin, griffonia simplicifolia, soybean agglutinin, peanut agglutinin, ricinus communis agglutinin isolectin i, griffonia simplicifolia isolectin b4, ulex europaeus isolectin i and phaseolus vulgaris agglutinin p have been documented in the subcellular compart ... | 1992 | 1374993 |
| fertilization failure of frozen mouse oocytes is not due to premature cortical granule release. | the proportion of mouse oocytes that were fertilized in vitro after storage at -196 degrees c in the presence of 1.5 m dimethylsulfoxide (dmso) was significantly lower than in unfrozen controls (39% vs. 81%). sperm failed to penetrate the zona pellucida of approximately 80% of the frozen oocytes that remained unfertilized. removal of the zona restored fertilization to control levels, indicating that changes induced in the zona during freezing and/or thawing were the primary cause of fertilizatio ... | 1992 | 1391317 |
| [alterations of ulex europaeus agglutinin-1 receptor in human colorectal benign and malignant tumors]. | the distributions and alterations of uea-1 receptor in 116 human colorectal mucosa, included 20 normal mucosa, 16 inflammatory and 14 hyperplastic polyps, 34 adenomas, and 32 carcinomas, were studied with abc technique. the results follow as: 1. there were positive staining of uea-1 in proximal colon and negative in distal colon and rectum; 2. the frequency and intensity of uea-1 receptor stained were increasing with the sequence of normal mucosa (7.7%), inflammatory polyps (25%), hyperplastic p ... | 1992 | 1395909 |
| developmental patterns of pdgf b-chain, pdgf-receptor, and alpha-actin expression in human glomerulogenesis. | expression of pdgf b-chain and the pdgf receptor beta-subunit (pdgfr beta) is detected immunocytochemically during the development of glomeruli in human kidneys of 54 to 105 days gestational age. during the early stages (vesicular, comma-shape and s-shape) of glomerulogenesis, pdgf b-chain is localized to differentiating epithelium of the glomerular vesicle, while pdgfr beta is expressed in the undifferentiated metanephric blastema, vascular structures, and interstitial cells. during this stage ... | 1992 | 1405322 |
| quantitative analysis of activated kupffer cells in viral hepatitis: application of computer image analysis for lectin histochemistry. | lectin histochemistry revealed that kupffer cells in the normal liver bound lectins such as concanavalin a (con a), ricinus communis agglutinin (rca) and wheat germ agglutinin (wga), but did not bind peanut agglutinin (pna), dolichos fibflorus agglutinin (dba), ulex europaeus agglutinin i (uea-i) or soybean agglutinin (sba). kupffer cells in viral liver diseases, however, bound the pna lectin and the binding was specific to kupffer cells in liver parenchyma. computer image analysis was performed ... | 1992 | 1406083 |
| simultaneous detection of ferritin and hiv-1 in reactive microglia. | using ferritin as a marker of reactive microglia, we demonstrated a close association between proliferation of reactive microglia and expression of human immunodeficiency virus type 1 (hiv-1) in brain tissue from autopsied cases of acquired immunodeficiency syndrome (aids). an increased number of ferritin-positive reactive microglia was observed in formalin-fixed paraffin-embedded brain sections from all 13 aids cases examined. similar findings were observed in brain tissue from other neurologic ... | 1992 | 1414282 |
| the morphologic characteristics of tumor blood vessels as a marker of tumor progression in primary human uveal melanoma: a matched case-control study. | nine morphologic patterns of tumor vessels were identified in eyes removed for ciliary body or choroidal melanoma by the examination of tissue sections stained with fluorescein-conjugated ulex europaeus i using laser scanning confocal microscopy. this technique also highlights intravascular tumor invasion. each of these nine morphologic patterns of tumor vessels also may be demonstrated by a modification of the periodic acid-schiff reaction, viewed with a green narrow band pass filter, but this ... | 1992 | 1427757 |
| phenotypic and ultrastructural characterization of an epithelial cell line established from rat thymic cultures. | an epithelial cell line (te-r 2.5) was established from a long-term culture of rat thymic epithelium. its epithelial nature was confirmed using anti-cytokeratin (ck) monoclonal antibodies (mab) and electron-microscopy. te-r 2.5 cells were reactive with k 8.13, k 8.12, ck 8, r-mc 18, r-mc 19 and mar 3 mab and bind ulex europaeus agglutinin i. based on the results of this study it was concluded that they possess the phenotype of subcapsular/perivascular or medullary epithelium. this was in accorda ... | 1992 | 1427975 |
| development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. | primary culture of rat brain endothelial cells is described, based on the method of c. c. w. hughes and p. l. lantos. the cells have been characterised using morphological and immunocytochemical techniques, and systematic studies undertaken to determine the optimal culture medium and conditions required to grow the cells at high purity on a variety of substrata. the endothelial cells have a spindle-shaped morphology, and proliferate as plaques from small clusters of cells associated with capilla ... | 1992 | 1429907 |
| high osmotic pressure for pneumocystis carinii london resin white embedding enables fine immunocytochemistry studies: i. golgi complex and cell-wall synthesis. | a method for embedding pneumocystis carinii in hydrophilic resin (london resin white) has been developed for immunocytochemistry studies. using high osmotic pressure (about 850 mosmol) from fixation to embedding, this method improved the preservation of the fine structure as well as the antigenicity of rabbit- and scid mouse-derived p. carinii. cytochemistry studies were performed using colloidal gold-conjugated lectins (concanavalin a, glycine max, ulex europaeus) that reacted with the cytoplas ... | 1992 | 1438133 |
| identification of sugar residues in human fetal olfactory epithelium using lectin histochemistry. | lectin-binding histochemistry was used to investigate the distribution and the changes of the glycoconjugate saccharidic moieties in the olfactory epithelium of human fetuses ranging from 8 to 12 weeks of gestation. it was found that the basal cells, the sustentacular cells and the olfactory neurons exhibit differences in oligosaccharide cellular content and distribution. differences in lectin binding was also demonstrated at the dendrite, cell body and axon of the receptor cells. from the 11th ... | 1992 | 1441890 |
| expression of glycoconjugates in the human fetal cochlea. | the distribution of glycoconjugates in the human fetal cochlea was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). the tectorial membrane (tm) in the 11- and 15-week-old human fetuses was labelled with wga, apa, rca120 and cona, but not with uea-i and hpa. in the 19-week-old fetuses, the reaction of the tm ... | 1992 | 1442009 |
| comparative lectin histochemistry on taste buds in foliate, circumvallate and fungiform papillae of the rabbit tongue. | taste buds (tb) in the foliate, circumvallate and fungiform papillae of the rabbit tongue were examined with lectin histochemistry by means of light (lm) and electron (em) microscopy. biotin- and gold-labeled lectins were used for the detection of carbohydrate residues in tb cells and subcutaneous salivary glands. at the lm level, the lectins of soybean (sba) and peanut (pna) react with material of the foliate and circumvallate taste pores only after pretreatment of the section with neuraminidas ... | 1992 | 1452451 |
| histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows. | the epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three holstein-friesian cows affected with chronic polypoid cystitis. except for ricinus communis (rca-i), almost all goblet cells in the whole crypt were positive to periodic acid-schiff (pas), alcian blue (ab) ph 2.5, ab ph 1.0, periodate borohydride-potassium hydroxide-pas (pb-koh-pas), ulex europaeus (uea-i), triticum vulgare (wga) and arachis hypogaea (pna) after neuraminidase digestion. t ... | 1992 | 1452812 |
| temporal lectin histochemical characterization of porcine small intestine. | a variety of biotinylated lectins was applied to formalin-fixed intestinal sections from isolator-reared pigs ranging in age from newborn through 12 weeks. lectin binding to brush borders of villus enterocytes, crypt enterocytes, and dome epithelium, and lectin reactivity within goblet cells and brunner's glands was semiquantified by microscopy and was used to estimate temporal changes in complex carbohydrates of enteric epithelium. although variability in binding scores often was observed among ... | 1992 | 1456535 |
| human sperm surface mapping with lectins. | ten fluorescein isothiocyanate (fitc)-linked lectins [bauhimia purpurea, concanavalin a, dolichos biflorus (dba), griffonia simplicifolia i, griffonia simplicifolia ii, maclura pomifera, arachis hypogea (pna), glycine max, ulex europaeus (uea) and triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. furthermore, ultrastructural loc ... | 1992 | 1466231 |
| [changes in gastrointestinal mucins caused by attapulgite. experimental study in rats]. | a histochemical study was carried out to evaluate the changes occurring in mucins secreted by the rat stomach and intestine following a 7 day-treatment with a cytoprotective clay: attapulgite. staining of gastrointestinal sections was performed with periodic acid-schiff reagent, alcian blue ph 2.5 and ph 1.0, and with lectin conjugated with horseradish peroxidase for detecting complex carbohydrates. when compared with controls, attapulgite induced an increase in carboxylic mucin content in the c ... | 1992 | 1493901 |
| histochemical reactivities of lectins and surfactant apoprotein in pulmonary adenocarcinomas and their metastases. | in an attempt to better understand the biologic behavior of neoplastic cell metastasis, a histochemical study with the use of six different lectins and a monoclonal antibody against human pulmonary surfactant apoprotein (pe-10) was carried out on primary adenocarcinomas of the lungs and their regional (usually lymphatic to lymph nodes or contralateral lung) and distant (usually hematogenous to extrathoracic organs) metastatic lesions of 54 postmortem cases. primary pulmonary adenocarcinomas were ... | 1992 | 1495933 |
| culture of endothelial cells from baboon and human glomeruli. | whereas mesangial and epithelial cells from glomeruli are commonly grown in vitro, there has been a failure to isolate and propagate human glomerular capillary endothelial cells. this study defines the conditions for the reproducible isolation and growth of homogeneous monolayers of primate (baboon and human) glomerular capillary endothelial cells. using selective media and growth factors, the following criteria were identified to optimize the isolation and proliferation of glomerular endothelia ... | 1992 | 1501407 |
| the growth of two murine hemangioendotheliomas intracranially, subcutaneously, and in culture, and their comparison with human cerebellar hemangioblastomas: morphological and immunohistochemical studies. | two thorium dioxide-induced murine hemangioendotheliomas, 42021 tct and 44347 tst, were grown subcutaneously (for up to 22 and 15 passages respectively) or intracranially (single passage) and were adapted to culture as a monolayer and, in a limited fashion, in an organ culture system or in rotary suspension. they remained viable and malignant following 20-21 years of storage in liquid nitrogen, and had ultrastructural similarities to human hemangioblastomas. the murine tumors were positive for g ... | 1992 | 1502883 |
| histochemical localization of glycoconjugates in the developing endolymphatic sac and vestibular end organs of the mouse. | the distribution of glycoconjugates in the endolymphatic sac (es) and vestibular end organs from the 12th gestational day (gd) in the developing mouse to the 6th day after birth was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 13th and 15th gd sections, the luminal contents in the fetal es were st ... | 1992 | 1518658 |
| ultrastructural and immunohistochemical studies of the intrahepatic peribiliary capillary plexus in normal livers and extrahepatic biliary obstruction in human beings. | ultrastructural and immunohistochemical study was conducted on the intrahepatic peribiliary capillary plexus in normal livers and in those with extrahepatic biliary obstruction. in both conditions, capillaries positive for ulex europaeus agglutinin i and type iv collagen were always present in the vicinity of the bile ducts. immunoelectron microscopy showed the presence of type iv collagen on the basal lamina of these capillaries; ulex europaeus agglutinin i was also positive on their cytoplasms ... | 1992 | 1544621 |
| flow cytometric analyses of lectin binding to pneumocystis carinii surface carbohydrates. | pneumocystis carinii obtained from infected rat lung homogenates was incubated with fluorescein isothiocyanate-conjugated lectins, counterstained with the nuclear stain, propidium iodide (pi), and analyzed by dual parameter histograms for lectin-associated green and pi-associated red fluorescence using a fluorescence-activated cell sorter. the presence of glucose/mannose moieties was evidenced by the binding of all organisms to concanavalin a and wisteria floribunda. from the lectin group specif ... | 1992 | 1556643 |
| distinction of human hookworm larvae based on lectin-binding characteristics. | a comparative study of lectin binding to ensheathed (enl3) and exsheathed (exl3) l3 larvae of necator americanus, ancylostoma duodenale and ancylostoma ceylanicum revealed a number of differences between these hookworm species. these differences could provide a novel approach to distinguish infective l3 larvae in field conditions. for example, binding of ulex europaeus agglutinin (uea) and ricinus communis agglutinin (rca120) to n. americanus enl3 distinguished them from those of a. duodenale an ... | 1992 | 1570175 |
| structures of the asparagine-linked oligosaccharide chains of human von willebrand factor. occurrence of blood group a, b, and h(o) structures. | the asparagine-linked oligosaccharide chains of human von willebrand factor (vwf) purified from pooled plasma were quantitatively liberated from the polypeptide moiety by hydrazinolysis. after n-acetylation, these were fractionated by paper electrophoresis and sequential chromatography on lectin-affinity columns of concanavalin a, phaseolus vulgaris erythrophytohemagglutinin, datura stramonium agglutinin, ricinus communis agglutinin 120, and ulex europaeus agglutinin i and on a bio-gel p-4 colum ... | 1992 | 1577715 |
| high affinity binding proteins for pancreatic polypeptide on rat liver membranes. | we report here the identification on rat liver plasma membranes and microsomes of proteins that bind pancreatic polypeptide (pp) with high affinity and specificity (plasma membranes: kd = 4.6 nm, bmax = 3.28 pmol/mg protein; microsomes: kd = 3.45 nm, bmax = 18.7 pmol/mg protein). these binding proteins appeared coupled to a g-protein, since 0.1 mm guanosine 5'-o-(3-thiotriphosphate) decreased the affinity by half. when 125i-labeled pp-binding protein complexes covalently cross-linked with disucc ... | 1992 | 1577769 |
| carbohydrates in the cell surface of hair cells from the guinea pig cochlea. | the presence of cell surface carbohydrates was investigated in intact, non-fixed outer hair cells (ohcs) of guinea pigs using fluorescein isothiocyanate (fitc) and rhodamine (tritc) lectins. by means of wheat germ agglutinin (wga) n-acetyl-d-glucosamine was shown in the entire ohc membrane, including the stereocilia. binding of wga in ohcs to neuraminic acid was excluded by preincubation with neuraminidase. moreover, fitc-limulus polyphemus, a specific lectin for neuraminic acid, showed no fluor ... | 1992 | 1581047 |
| biochemical characterization of a subtype pancreatic cholecystokinin receptor and of its agonist binding domain. | this study was undertaken in order to improve photoaffinity labelling efficiency of pancreatic cholecystokinin receptor by the cleavable probe 125i-asd-(thr28,ahx31)-cck-25-33 and to further characterize the denaturated receptor and its agonist binding domain. membrane bound pancreatic cholecystokinin receptor was specifically labelled by 125i-asd-(thr28,ahx31)-cck-25-33 as a component of mr approximately 85,000-100,000. the efficiency of the photolabelling was 3-4%. performing photolysis on [12 ... | 1992 | 1583623 |
| subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization. | using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rer) in goblet cells of the rat colon. in this cell type, special rer regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. the fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, helix pomatia, griffonia simplicifolia i-a4 and ... | 1992 | 1607641 |
| origin of cells in the coronary intima during acute vascular rejection of the transplanted human heart. | acute vascular rejection of the coronary arteries in human heart transplants is characterized by a lymphocytic infiltrate and a thickened intima that contains numerous highly vacuolated cells. the origin of the vacuolated cells has been controversial. in this immunocytochemical and electron microscopic study of four patients with acute vascular rejection, the predominant cells in the coronary artery intima were host-derived lymphocytes and highly vacuolated smooth muscle cells. lymphocytic infil ... | 1992 | 1610858 |
| purification and characterization of a salmonella typhimurium agglutinin from gut mucus secretions. | one of the earliest events in salmonella typhimurium pathogenesis seems to be the interaction of the bacterium with the mucus of the gut. after exposing s. typhimurium to guinea-pig colon, we were able to demonstrate by electron microscopy that s. typhimurium bacteria were trapped on, or in, the mucus layer. specific components isolated from crude mucus secretions were found to aggregate the bacteria. the degree of bacterial aggregation was dependent on the protein concentration of the crude muc ... | 1992 | 1630296 |
| kaposi sarcoma in okinawa. | the authors encountered six patients with kaposi sarcoma in okinawa; one was classic type, two were associated with adult t-cell leukemia (atl), one was with multiple myeloma, and two were with acquired immune deficiency syndrome (aids). in the classic type, many nodular lesions were seen on the skin of the extremities and a few on the trunk, some of which were ulcerated. most lesions regressed in 1.5 years. in four other patients (three without aids and one with aids), many plaques and a few no ... | 1992 | 1643618 |
| evidence that the major outer membrane protein of chlamydia trachomatis is glycosylated. | the major outer membrane protein (momp) of chlamydia trachomatis was determined to be a glycoprotein on the basis of susceptibility to glycosidase digestion and the presence of carbohydrate by staining and radiolabeling. the momp of the serovar l2 organisms was isolated by electroelution from the protein band excised from the gel after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the incubation of momp with n-glycosidase f, an endoglycosidase that cleaves the n-glycan, a ... | 1991 | 1645328 |
| ultracytochemical characteristics of cultured goat brain microvascular endothelial cells [corrected]. | this ultrastructural study was undertaken to determine the localization of cytochemically demonstrable blood-brain barrier (bbb)-associated enzymatic activities and of some nonenzymatic constituents in goat [corrected] brain microvascular endothelial cells (ecs) growing in vitro. positive reactions for alkaline phosphatase (ap), 5'-nucleotidase (5'n), transport atpase (na+,k(+)-atpase), and adenosine diphosphatase (adpase) were present on both apical and basolateral plasma membranes (pms) of the ... | 1991 | 1655877 |
| cytomegalovirus vasculitis accompanied by an exuberant fibroblastic reaction in the intestine of an aids patient. | a case of cytomegalovirus (cmv) vasculitis in the intestine of a patient with acquired immune deficiency syndrome (aids) is reported. the distal jejunum and ileum had multiple well-demarcated mucosal ulcers and microscopic examination revealed an unusual, exuberant fibroblastic proliferation in the ulcer base. amidst these fibroblasts, there were several small blood vessels of which the endothelial cells contained cytomegalic inclusion bodies. the lesion showed a kaposi's sarcoma-like appearance ... | 1991 | 1664639 |
| mammalian cell lines can be efficiently established in vitro upon expression of the sv40 large t antigen driven by a promoter sequence derived from the human vimentin gene. | the aim of this study was to investigate a new method to enhance the efficiency to create mammalian cell lines. cell immortalization was achieved by intranuclear microinjection of a recombinant dna construct composed of a constitutive promoter controlling the genes encoding immortalizing proteins; the sequences coding for the large t and small t antigens were fused downstream of regulatory elements from the vimentin gene, the activation of which characterizes the vast majority of cells growing i ... | 1991 | 1668064 |
| collagenase production by immortalized human aortic endothelial cells infected with simian virus 40. | human aortic endothelial cells, isolated at autopsy from a 52-year-old male dying from lung cancer, were treated with simian virus 40 (sv40). one colony was isolated from the infected endothelial cell culture 4 weeks after infection. the cells expressed sv40 large t antigen and p53 protein (p53) in their nuclei but lacted the characteristics of a transformed phenotype. the cells grew well in a monolayer over the 97th passage and exhibited factor viii-related antigen, ulex europaeus 1 agglutinin ... | 1991 | 1675513 |
| ultrastructural localization of keratin and alpha-l-fucose in human eccrine sweat glands. | human eccrine sweat glands were embedded in lowicryl k4m. cytokeratin proteins and blood group h antigen were localized by applying a postembedding immunogold method using a monoclonal antikeratin antibody and the lectin ulex europaeus i. the antikeratin antibody labeled intermediate filaments in the secretory coil and dermal duct. within dark secretory cells bundles of filaments criss-crossing the cell were labeled. within the luminal cells of the dermal duct filaments arranged parallel to the ... | 1990 | 1690530 |
| spontaneous transformation and immortalization of human endothelial cells. | a new cell line from the human umbilical vein has been established and maintained for more than 5 yr (180 generations; 900 population doublings). this strain, designated ecv304, is characterized by a cobblestone monolayer growth pattern, high proliferative potential without any specific growth factor requirement, and anchorage dependency with contact inhibition. karyotype analysis of this cell line reveals it to be of human chromosomal constitution with a high trisomic karyotype (mode 80). ultra ... | 1990 | 1690702 |
| cellular localization of an hiv-1 antigen in subacute aids encephalitis using an improved double-labeling immunohistochemical method. | among 102 brains obtained from patients with acquired immune deficiency syndrome (aids), 34 cases with subacute aids encephalitis were characterized by immunohistochemistry using an antibody that binds to a human immunodeficiency virus-1 (hiv-1) envelope glycoprotein, gp41. this glycoprotein was detected in mononucleated and/or multinucleated cells in 90% of adult and 50% of pediatric brains with subacute aids encephalitis. in addition, many gp41-positive cells with bipolar or multipolar process ... | 1990 | 1693470 |
| carbohydrate moieties in recombinant human thyroid peroxidase: role in recognition by antithyroid peroxidase antibodies in hashimoto's thyroiditis. | we studied the oligosaccharide moieties of recombinant human thyroid peroxidase (htpo) expressed in chinese hamster ovary (cho) cells, and the role of these glycans in htpo antigenicity in hashimoto's thyroiditis. to determine whether htpo carbohydrate moieties were n-linked, o-linked, or both, and to obtain information about the characteristics of the carbohydrate component(s), we digested htpo with deglycosylating enzymes of varying specificity. proteins in cho-tpo cells were labeled with [35s ... | 1990 | 1693564 |
| an immunohistochemical and ultrastructural study of the sinusoids of hepatocellular carcinoma. | the sinusoids of 30 human hepatocellular carcinomas of various types were examined by electron microscopy and histochemically for binding to the ulex europaeus lectin (uea1). a population of sinusoidal macrophages was identified with an antibody to lysozyme (muramidase). the uea1 binding was negative in normal sinusoids but positive in the tumor vessels. macrophages resembling kupffer cells were found within the tumor vessels but in smaller numbers than in either normal or cirrhotic liver tissue ... | 1990 | 1695542 |
| lectin histochemical characteristics of the canine female mammary gland. | twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ... | 1990 | 1700652 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| identification of endothelial and mesothelial cells in human omental tissue and in omentum-derived cultured cells by specific cell markers. | human omental tissue has been used as a source for the isolation and cultivation of microvascular endothelial cells, but also for mesothelial cells. since both cell types have several morphologic and functional features in common, concerns were raised whether endothelial cells can be separated from mesothelial cells by the methods described for the isolation of microvascular endothelial cells. in the present study, endothelial cells were identified in the capillaries of native human omentum by s ... | 1990 | 1701509 |
| endothelial markers in malignant vascular tumours of the liver: superiority of qb-end/10 over von willebrand factor and ulex europaeus agglutinin 1. | a new monoclonal antibody, qb-end/10, raised against the cd34 antigen in human endothelial cell membranes and haemopoietic progenitor cells, was studied for its usefulness as a marker of neoplastic vascular cells in 21 angiosarcomas and seven malignant haemangioendotheliomas of the liver. qb-end/10 was both more sensitive and more specific than von willebrand factor (vwf) and ulex europaeus 1 agglutinin (uea-1) in labelling endothelial cells and it did not cross react with epithelia as uea-1 oft ... | 1991 | 1705261 |
| difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues. | twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ... | 1990 | 1705925 |
| detection of blood group a and h-related antigens in normal and neoplastic bladder epithelium: a comparative study using monoclonal antibodies with defined fine specificities. | three monoclonal anti-blood group h and four monoclonal anti-blood group a antibodies directed at the blood group antigens on type 1 or type 2 backbone structures were evaluated as immunohistochemical reagents by indirect immunofluorescence of normal and neoplastic bladder epithelia. the results were compared with fluorescence using polyclonal rabbit anti-a or h sera or ulex europaeus lectin. the monoclonal antibodies gave less intense or more restricted immunofluorescence than the conventional ... | 1991 | 1707648 |
| wheat germ agglutinin and other selected lectins increase synthesis of decay-accelerating factor in human endothelial cells. | decay accelerating factor (daf) is a cell-surface phosphatidylinositol-anchored protein that protects the cell from inadvertent complement attack by binding to and inactivating c3 and c5 convertases. we have measured daf on human umbilical vein endothelial cells (huvec) by immunoradiometric assay after its removal by phosphatidylinositol-specific phospholipase c or nonidet p-40 detergent extraction and have previously demonstrated that daf synthesis can be stimulated by phorbol ester activation ... | 1991 | 1716283 |
| bronchiolar metaplasia and ulex europaeus agglutinin i (uea-i) affinity in mycoplasma hyopneumoniae-infected lungs of six pigs. | | 1991 | 1722926 |
| the characterization of lectin-binding proteins of chlamydia trachomatis as glycoproteins. | the 18 kda and 32 kda lectin binding proteins of chlamydia trachomatis were characterized as glycoproteins by treatments with glycosidases. the proteins of the serovar l2 whole cell lysate were separated by sds-page and transferred to nitrocellulose paper. after treatment with an enzyme, the proteins were reacted with a biotinylated lectin. each of the endoglycosidases tested affected the binding of the lectin to the protein. pngase f inhibited the binding of dolichos biflorus agglutinin (dba), ... | 1991 | 1724547 |
| quantitative dot blot analyses of blood-group-related antigens in paired normal and malignant human breast tissues. | membranes were prepared from 31 breast-cancer specimens and adjacent mammary tissues, dot-blotted to nitrocellulose paper, and reacted with monoclonal antibodies (mabs) (a, b, lewis a, lewis b, sialylated lewis a, lewis x, and lewis y) and lectins (ulex europaeus, peanut agglutinin) having various blood-group specificities. the expression of epithelial membrane antigen was assayed with mab ma5. the ratio of breast-cancer to normal mammary membrane preparations (c/n ratios) of these reagents was ... | 1992 | 1728614 |
| malignant proliferative angioendotheliomatosis or angiotropic lymphoma associated with a soft-tissue lymphoma. | we report a 63-year-old man with violaceous nummular patches on the trunk. histopathologic studies were consistent with a diagnosis of malignant angioendotheliomatosis or angiotropic lymphoma. immunohistochemical study of skin was positive for uchl-1 antigen and leukocyte common antigen and negative for l-26, ulex europaeus lectin i, vimentin, cytokeratin, and epithelial membrane antigen. ultrastructural study ruled out an endothelial origin of the neoplastic cells. these data confirmed the diag ... | 1992 | 1732314 |
| lectin binding sites in developing mouse limb buds. | the binding sites of the following biotinylated lectins were demonstrated in serial paraffin sections of fore- and hindlimb buds from day-9 to day-16 mouse embryos with the avidin-biotin-peroxidase complex (abc) procedure: concanavalin a (con a), soybean agglutinin (sba), wheat germ agglutinin (wga), peanut agglutinin (pna), ricinus communis agglutinin i (rca), ulex europaeus i agglutinin (uea), and dolichos biflorus agglutinin (dba). alternating neighbouring sections were used to compare the di ... | 1991 | 1741479 |
| lectin binding to gp60 decreases specific albumin binding and transport in pulmonary artery endothelial monolayers. | the effect of albumin binding to cultured bovine pulmonary artery endothelial cell (bpaec) monolayers on the transendothelial flux of 125i-labelled bovine serum albumin (bsa) was examined to determine its possible role on albumin transcytosis. the transport of 125i-bsa tracer across bpaec grown on gelatin- and fibronectin-coated filters (0.8 microns pore diam.) was affected by the presence of unlabelled bsa in the medium in that transendothelial 125i-bsa permeability decreased, reaching a 40% re ... | 1991 | 1744181 |
| differential lectin binding on walls of thoraco-cervical blood vessels and lymphatics in rats. | lectin binding in the walls of large to medium-sized blood vessels and lymphatics in the rat thoraco-cervical region was examined histochemically. the tunica intima of the aorta and superficial cervical artery showed positive reactions with wheat germ agglutinin (wga) and concanavalin a (cona) but not with dolichus biflorus agglutinin (dba). the tunica media of the aorta exhibited intense wga binding, especially on the smooth muscle cells, but the tunica media of the superficial cervical artery ... | 1991 | 1758681 |
| morphological study of lectin binding in the rabbit temporomandibular joint disc. | glycoconjugates of the extracellular matrix are important for the normal mechanical functions of connective tissue structures such as the temporomandibular joint disc. since lectins are known to bind to sugar residues with high affinity, a variety of lectins were used to study the presence and distribution of glycoconjugates in the temporomandibular joint disc. discs were removed from 6 to 8-month-old rabbits and either sectioned in a cryostat and processed for light microscopy or fixed in 2% gl ... | 1991 | 1783561 |
| demonstration of lymphatics in human synovial tissue. | using a cocktail of monoclonal antibodies pal-e and de-u-10 (anti-desmin), combined in double labelling techniques with the lectin ulex europaeus agglutinin i (ueai), vessels consistent with lymphatics were demonstrated in normal human synovial tissue. these vessels were negative for the monoclonal cocktail and positive for ueai, were thin-walled and were located close to deep arterioles and venules as expected. elastin was not found to assist identification of lymphatics in synovium. in rheumat ... | 1991 | 1784883 |
| changes in cell-surface carbohydrates of trypanosoma cruzi during metacyclogenesis under chemically defined conditions. | highly purified lectins with specificities for receptor molecules containing sialic acid, n-acetylglucosamine (d-glcnac), n-acetylgalactosamine (d-galnac), galactose (d-gal), mannose-like residues (d-man) or l-fucose (l-fuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite trypanosoma cruzi during metacyclogenesis under chemically defined conditions. of the d-galnac-binding lectins, bs-i selectively agglutinated metacyclic trypomastigotes, mpl was selective ... | 1991 | 1791438 |
| lectin histochemistry of feline sphingomyelinosis. | the brain from a siamese cat with sphingomyelinosis was examined with lectin histochemistry. swollen neurons were stained with canavalia ensiformis agglutinin (con a). some of them were also stained with ricinus communis agglutinin-i (rca-i) and ulex europaeus agglutinin-i (uea-i). a small number of axonal spheroids and glia cells were positive for con a, rca-i, uea-i and wheat germ agglutinin. control tissues were weakly stained with con a, but not with any of the other lectins. these results i ... | 1991 | 1806052 |
| cultivation of human dermal microvascular endothelial cells in vitro: immunocytochemical and ultrastructural characterization and effect of treatment with three synthetic retinoids. | a new reliable and reproducible technique to culture endothelial cells from the small vessels and capillaries of human skin is introduced, and proliferation and differentiation of the growing cells are characterized. the endothelial origin of the culture cells was confirmed by light- and electron microscopy and by labelling with ulex europaeus agglutinin i and an antibody against factor viii-related antigen. further immunocytochemical characterization showed that 92-100% of the cells were positi ... | 1991 | 1831018 |
| expression of abh blood group antigens, receptors of ulex europaeus agglutinin i, and factor viii-related antigen on sinusoidal endothelial cells in adenomatous hyperplasia in human cirrhotic livers. | adenomatous hyperplasia, a hyperplastic parenchymal nodule in the cirrhotic liver, has been presumed to be a preneoplastic lesion in human hepatocarcinogenesis. in this study, phenotypes of the sinusoidal endothelium were examined in adenomatous hyperplasia, hepatocellular carcinoma, cirrhosis, chronic active hepatitis, and normal livers. adenomatous hyperplasia (n = 74) was histologically classified into two types: ordinary (n = 35) and atypical (n = 39). while the former lacked hepatocellular ... | 1991 | 1851720 |
| defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins. | the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ... | 1991 | 1862641 |
| lectin histochemistry of feline polyglucosan bodies. | lectin histochemistry was conducted to identify sugar residues of the polyglucosan bodies (pgb) in the feline brain. pgb stained with concanavalia ensiformis agglutinin (con a) and peanut agglutinin, but not with soybean agglutinin, wheat germ agglutinin, dolichos biflorus agglutinin, ricinus communis agglutinin or ulex europaeus agglutinin. in addition, pgb bound to con a after periodate oxidation. these results suggest that feline pgb contain mannose and galactose in addition to glucose and th ... | 1991 | 1865024 |
| carbohydrate analysis of human von willebrand factor with horseradish peroxidase-conjugated lectins. | human von willebrand factor (vwf) immobilized on a polyvinylidene difluoride membrane was subjected to binding assay with a series of horseradish peroxidase-conjugated lectins. the protein was reactive with concanavalin a, ricinus communis agglutinin 120, wheat germ agglutinin and ulex europaeus agglutinin i (uea-i) but not with peanut agglutinin before sialidase treatment. these reactivities were consistent with the major oligosaccharide structure reported except for uea-i. the reactivity with ... | 1991 | 1872845 |
| metastatic angiosarcoma of the brain. | two patients with metastatic angiosarcoma of the brain are described. in one, a 17-year-old man, the tumor was located at the pineal region and exhibited significant vascularity. it was sensitive to radiation therapy and disappeared after radiation of 50 gy; however, it recurred after 1 year and a new lesion was found in the liver. the other patient is a 31-year-old woman who experienced sudden onset of headache. computed tomography scan revealed three separate masses in the brain. one tumor was ... | 1991 | 1901178 |
| further studies of lectin binding by villous and extravillous trophoblast in normal and pathological pregnancy. | examination of human pregnancy tissues with a panel of lectins provides the opportunity to probe different aspects of carbohydrate structure. nine biotinylated lectins [concanavalin a (con a), wheat germ agglutin (wga), lens culinaris a (lch-a), pisum sativum (psa), phaseolus vulgaris (pha-e and pha-l), ulex europaeus 1 (uea1), griffonia simplicifolia (gsi and gsii)] were used to investigate the lectin binding of human trophoblast in normal, tubal, and molar pregnancy. all lectins except uea1 bo ... | 1991 | 1917274 |
| glycoconjugates in the human fetal endolymphatic sac as detected by lectins. | the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ... | 1991 | 1919336 |
| fucosylated glycoconjugates in human dorsal root ganglion cells with unmyelinated axons. | the same subset of small neurons in human dorsal root ganglia (drg) was recognized by both of the two probes binding to fucosylated residue. ulex europaeus agglutinin i (uea-1) lectin and anti-fucosyl gm1 antibody, although these probes bind to different glycoconjugates. uea-1 lectin also bound to unmyelinated axons in drg and in biopsied sural nerve, but not to any neurons or unmyelinated axons in the sympathetic ganglia. thus uea-1 lectin and anti-fucosyl gm1 antibody may be used as specific p ... | 1991 | 1922925 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |
| comprehensive lectin histochemistry of normal and neoplastic human choroid plexus cells: alternation of lectin-binding patterns through neoplastic transformation. | lectin histochemistry of the normal and neoplastic human choroid plexus cells [six choroid plexus papillomas (cpps) and three choroid plexus carcinomas (cpcs)] was performed using eight representative lectins to study the development of sugar chain structures and also to determine whether lectins were useful for a histopathological diagnosis of choroid plexus neoplasms (cpns). the normal choroid plexus cells reacted with ricinus communis (rca-i). canavalia ensiformis (con a), limax flavus (lfa) ... | 1991 | 1927268 |
| glycoconjugate histochemistry of xenopus laevis fundic gland with special reference to mucous neck cells during development. | mucous neck cells (mncs) of the fundic gland are phylogenetically thought to have first appeared in amphibians. we studied the origin and differentiation of mncs in fundic glands of xenopus laevis. by means of lectin histochemical methods using griffonia simplicifolia agglutinin-ii (gsa-ii), mncs were detected specifically in fundic glands of adult x. laevis. mucous granules of mncs were labeled by gsa-ii-colloidal gold (cg) staining. other cells such as surface mucous cells (smcs), oxynticopept ... | 1991 | 1928755 |
| deantigenation of human erythrocytes by bacterial glycosidases--evidence for the noninvolvement of medium-sized glycosphingolipids in the dolichos biflorus lectin hemagglutination. | fresh human a1 erythrocytes, washed and pretreated in phosphate buffer with or without papain, were incubated at 37 degrees c with blood group-degrading enzymes from the human fecal ruminococcus torques strain ix-70. the effects were assayed as changes in hemagglutination patterns, and blood group activities of alkali stable glycolipid extracts from the enzyme-treated cells using dolichos biflorus anti-a1 lectin, ulex europaeus type 1 anti-h lectin, and various monoclonal anti-a antibodies. hemo ... | 1991 | 1929400 |
| blood group antigen expression in developing pancreas and in induced pancreatic cancer cells in syrian hamsters. | expression of blood group-related antigens and the binding pattern of ulex europaeus-i (uea-i) in fetal and newborn hamster pancreases was examined immunohistochemically by monoclonal antibodies (moabs) against blood group antigens and by peroxidase-conjugated uea-i. the fetal hamster pancreas was detected histologically at the 12th day of gestation. moabs a, b and le(a) were not immunoreactive with pancreatic cells in any stage of development nor were they after birth. le(x) was first expressed ... | 1990 | 1969774 |
| phenotypic relationships of prostatic intraepithelial neoplasia to invasive prostatic carcinoma. | thirty-one snap-frozen human prostate specimens containing examples of benign hyperplasia, prostatic intraepithelial neoplasia (pin), and invasive carcinoma were analyzed using a panel of 24 antibodies and one lectin. twenty-seven additional routinely processed radical prostatectomy specimens were studied using selected probes known to work on formalin-fixed paraffin-embedded material. three probes, anticytokeratin ka4, anti-vimentin v9, and the lectin from ulex europaeus (uea-1), demonstrated p ... | 1991 | 1987760 |
| a novel 43-kda glycoprotein is detected in the nucleus of mammalian cells by autoantibodies from dogs with autoimmune disorders. | we have characterized a new antibody specificity in a panel of sera from dogs developing systemic lupus erythematosus (sle) or clinically related autoimmune disorders. this antibody stains in a speckled fashion the nucleus of cells of different mammalian origins. the target antigen is a basic (pi 9.2) nuclear polypeptide with an apparent molecular weight of 43 kda (p43) which is detected in various mammalian cell nuclei. p43, as studied in hela cells, appears to be cell cycle-independent. it is ... | 1991 | 1995302 |
| role of abh blood group antigens in the stimulation of a dids-sensitive ca2+ influx pathway in human erythrocytes by ulex europaeus agglutinin i and a monoclonal anti a1 antibody. | of eleven agglutinating lectins tested, only one, ulex europaeus agglutinin i (uea1), stimulated ca2+ uptake in quin2-loaded erythrocytes by about 2-fold. uea1 is known to be an alpha-l-fucose and abh blood group specific lectin. the 45ca2+ influx induced by uea1 was absent in the presence of extracellular fucose (5 and 15 mm) and depended on the abh blood group of the donor, the stimulatory potency of the lectin decreasing in the order h greater than a2 greater than a1. ca2+ entry blockers, suc ... | 1991 | 2001409 |
| culture and characterization of microvascular endothelial cells derived from human brain. | primary cultures of human cerebral endothelial cells were established from microvessels isolated from cortical fragments removed at surgery for seizure disorder and from brains at autopsy. a uniform population of cells growing in close association to each other formed confluent monolayers by 7 to 10 days in culture. they contained factor viii/von willebrand antigen, the most specific marker for cells of endothelial origin, and showed lectin-binding sites for ulex europaeus agglutinin characteris ... | 1991 | 2002659 |
| serine protease immunohistochemistry and lectin histochemistry in the small intestine of weaned and unweaned pigs. | the distribution of goblet cells containing serine protease and of those binding the lectin ulex europaeus agglutinin-1 (uea-1) in the pig small intestine is altered during the period after weaning. goblet cells exhibiting binding of other lectins were not altered. these alterations and other changes in goblet cells may reflect the effects of a hypersensitivity response to dietary antigen. | 1991 | 2047582 |