| inhibition of ige and compound 48/80-induced histamine release by lectins. | lectins from ricinus communis and glycine max, as well as wheat germ agglutinin and concanavalin a, caused a dose-dependent release of histamine from mast cells present in the mixed peritoneal cells from the rat. in addition, histamine release in an ige-mediated and a compound 48/80-mediated reaction was inhibited in cells which had been pretreated with these lectins. with concanavalin a and the r. communis lectin both effect were prevented by the addition of the appropriate monosaccharides to t ... | 1975 | 51003 |
| observations on the reaction of en(a-)cells with sophora japonica haemagglutinin. | | 1975 | 126538 |
| involvement of glycoconjugates in insulin-receptor interactions. studies in liver plasma membranes of control and diabetic mice. | the involvement of glycoconjugates in the insulin-receptor interactions in mouse liver is tested by digestions of membranes with various enzymes. trypsin decreased the binding of [125i]insulin to liver membranes. after digestion with beta-galactosidase no ""high affinity'' receptor sites could be detected. the effects observed with plant lectins confirm the involvement of galactoconjugates in the insulin binding process. sophora japonica and ricinus communis lectins (with galactose specificity) ... | 1978 | 698217 |
| studies on the interaction of the sophora japonica lectin and concanavalin a with erythrocytes and lymphocytes. | the agglutinating activity of lectins from the seeds of sophora japonica and canavalia ensiformis (concanavalin a) with human and murine erythrocytes and lymphocytes have been compared to one another and related to the mitogenic and immunosuppressive properties of these purified proteins. the s. japonica lectin, which demonstrates blood group specificity, is more active than concanavalin a with human erythrocytes, but has a much lower reactivity than concanavalin a with murine red blood cells. f ... | 1976 | 955676 |
| lectins from wistaria floribunda seeds and their effect on membrane fluidity of human peripheral lymphocytes. | two lectins were isolated from wistaria floribunda seeds. one is a strong mitogen against human peripheal lymphocytes and has been purified in the previous paper (toyo-shima s., y., nakano k., tonomura, a., and osawa t. (1971) biochemistry 10, 4457). the other, which is a strong hemagglutinin being devoid of mitogenic activity against normal lymphocytes, has been purified in this paper by affinity chromatography on a sepharose 6b column followed by deae-sephadex column chromatography. both lecti ... | 1975 | 1112822 |
| [the modifying action of sophora japonica and an antioxidant vitamin complex on acute radiation injury to the body]. | in experiments with mongrel male rats exposed to whole-body gamma-radiation (7, 9 and 11 gy) the radioprotective effect of enterally administered sophora japonica and its combination with an antioxidant vitamin complex was investigated. the radioprotective agents applied were shown to produce a synergistic effect. | 1992 | 1448565 |
| [determination of lectin receptors on the surface membranes of lymphoid cells]. | the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ... | 1990 | 1692358 |
| difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues. | twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ... | 1990 | 1705925 |
| a study on the mutagenicity of 102 raw pharmaceuticals used in chinese traditional medicine. | the mutagenic potential of traditional chinese drugs was studied. after extraction with boiling water and frozen vacuum drying, preparations from 102 raw drugs were tested with the ames test, and the micronucleus and chromosomal aberration assays in mice in vivo. astragalus mongholicus bunge (+s9), sophora japonica l. (+/- s9) and eucommia ulmoides oliv. (+/- s9) were found to significantly induce his+ revertants in salmonella typhimurium ta98 and/or ta100. besides these 3, 10 other extracts wer ... | 1991 | 2027343 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| binding of the blood group-reactive lectins to human adult kidney specimens. | the binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. the blood group a-reactive lectins, all specific for alpha-d-n-acetylgalactosamine (galnac), helix aspersa (haa), helix pomatia (hpa), and griffonia simplicifolia i-a4 (gsa-i-a4) agglutinins bound to the endothelium in specimens with blood groups a and ab. in other samples, these lectins reacted predominantly with tubu ... | 1990 | 2297076 |
| [the modifying action of the japanese pagoda tree (sophora japonica) and pantocrine in radiation lesions]. | a study was made of the effect of sophora japonica and pantocrine on irradiated (2.5 gy) human lymphoblastoid cells. the radioprotective effect was manifested with the preparations injected separately after irradiation. the highest radioprotective effect was produced by the mixture of the preparations, the injection 15 min after irradiation being more effective than preinjection. the protective effect of the agents was studied on mongrel mice after the administration thereof for the purposes of ... | 1990 | 2349374 |
| glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins. | the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ... | 1988 | 2466021 |
| saccharide residues in human gingiva as revealed with fluorochrome-coupled lectins. | the histochemical binding of 16 fluorochrome-conjugated lectins to human marginal gingiva was investigated. of a total of 14 galactose/n-acetylgalactosamine (gal/galnac)-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), and helix aspersa agglutinins (haa) were blood group a-reactive whereas griffonia simplicifolia i-b4 (gsa-i-b4) and sophora japonica (sja) agglutinins were blood group b-reactive. hpa, haa and gsa-i-b4 bound to all suprabasal epithelial cells and to vascular endothe ... | 1989 | 2542513 |
| ultrastructure and lectin histochemistry of equine cutaneous histiolymphocytic lymphosarcomas. | tissues from subcutaneous lymphosarcomas and regional lymph nodes were examined by light and electron microscopy and by lectin histochemistry. tumors were composed of two major cell types: small lymphocytes with few organelles and pleomorphic histiocytic cells with undulant surfaces, large numbers of cytoplasmic vacuoles, and many mitochondria with large crystalline inclusions. a large gram-positive coryneform bacterium was isolated from tumor nodules but was not identified morphologically in tu ... | 1989 | 2588437 |
| mitogenic lectins bind to the antigen receptor on human lymphocytes. | the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ... | 1989 | 2703017 |
| demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins. | the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ... | 1989 | 2757235 |
| reactivity of visceral yolk sac endoderm with lectins: changes related to age and species. | the expression of receptors for lectins reacting with extraembryonal endoderm was compared between mouse and rat at different stages of gestation. fluorescein-conjugated helix pomatia agglutinin (hpa), dolichos biflorus agglutinin (dba), sophora japonica agglutinin (sja), peanut agglutinin (pna), bandeiraea simplicifolia agglutinin 1 (bsa-1) and gold-conjugated dba and pna were used. it was shown that the rat visceral endoderm does not express the receptors for hpa, while the mouse tissue does. ... | 1989 | 2762737 |
| alterations in glycoconjugate expression following malignant transformation of human oropharyngeal squamous mucosa. | malignant transformation of the squamous mucosa in the human oropharynx may be accompanied by alterations in carbohydrate production. glycoconjugate expression was assessed by examining lectin-binding patterns and abh isoantigen expression in human biopsy specimens from nine cases of carcinoma in situ and ten cases of invasive squamous cell carcinoma as well as adjacent normal squamous mucosa. ten different fluorescein-conjugated lectins were used. each lectin binds to specific nonreducing end-t ... | 1989 | 2913429 |
| the role of carbohydrates in the igg binding and suppressive activities of shed human fc receptors. | igg fc receptor (fc gamma r) shed from human peripheral mononuclear cells and purified by igg affinity chromatography bound to cona, pisum sativum, ulex europeus and peanut lectins. the soluble fc gamma r was not absorbed by wheat germ, soyabean, lentil, sophora japonica or helix pomotia lectins. out of 14 different sugars, none interfered with the interaction between igg fc and membrane fc gamma r. these results show that shed fc gamma r had glucosyl, mannosyl, fucosyl and galactosyl groups, bu ... | 1986 | 2944472 |
| dolichos biflorus agglutinin (dba) reacts selectively with mast cells in human connective tissues. | dolichos biflorus agglutinin (dba) binds to n-acetyl-d-galactosamine (galnac) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen a. in cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled dba bound preferentially to fusiform connective tissue cells and to certain epithelial cells. the connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. double l ... | 1988 | 3138306 |
| haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family. | twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ... | 1988 | 3377987 |
| [lectin receptors in the salivary glands of the rat during postnatal ontogenesis]. | distribution of lectin-binding sites in rat submandibular and sublingual salivary glands during postnatal development has been investigated. lectin preparations include con a, lentin lectin, castor beans agglutinin, peanut, soybean and sophora japonica agglutinins, wheat germ agglutinin and lectin from the bark of laburnum anagyroides. the direct and indirect peroxidase techniques are used. according to the similarities of histochemical patterns, all lectins are divided into four groups. besides ... | 1986 | 3535744 |
| the beta 1----2-d-xylose and alpha 1----3-l-fucose substituted n-linked oligosaccharides from erythrina cristagalli lectin. isolation, characterisation and comparison with other legume lectins. | the carbohydrate moieties of erythrina cristagalli lectin were released as oligosaccharides by hydrazinolysis, followed by n-acetylation and reduction with nab3h4. fractionation of the tritium-labelled oligosaccharide mixture by bio-gel p-4 column chromatography and high-voltage borate electrophoresis revealed that it is composed of five neutral oligosaccharides. structural studies by sequential exoglycosidase digestion in combination with methylation analysis and two-dimensional 1h-nmr showed t ... | 1987 | 3609010 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| reactivity of five n-acetylgalactosamine-recognizing lectins with preimplantation embryos, early postimplantation embryos, and teratocarcinoma cells of the mouse. | the expression of receptors for n-acetylgalactosamine-recognizing lectins, namely helix pomatia agglutinin (hpa), sophora japonica agglutinin (sja), bauhinia purpurea agglutinin (bpa), vicia villosa agglutinin (vva), and wistaria floribunda agglutinin (wfa) was studied in early mouse embryos and teratocarcinoma cells. each of these lectins as well as dolichos biflorus agglutinin (dba) bound differently to early embryonic cells, with the exception of vva and wfa which showed indistinguishable rea ... | 1985 | 4018458 |
| lectin binding affinities of induced pancreatic lesions in the hamster model. | we examined the binding pattern of nine lectins to n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic lesions in syrian hamsters. these lectins were arachis hypogaea (pna), dolichos biflorus (dba), griffonia simplicifolia i (gs-i), helix aspersa (haa), helix pomatia (hpa), sophora japonica (sja), ricinus communis i (rca-i), triticum vulgaris (wga) and ulex europaeus i (uea-i). all of the lectins reacted in untreated control hamsters to varying intensities with cytoplasmic components of acin ... | 1985 | 4064253 |
| purification and properties of the hemagglutinin from sophora japonica seeds. | | 1974 | 4203907 |
| structures of the galactomannans from seeds of annona muricata, arenga saccharifera, cocos nucifera, convolvulus tricolor, and sophora japonica. | | 1971 | 4339945 |
| interaction of lectins with neisseria gonorrhoeae. | fifty-four strains of neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. dolichos biflorus, sophora japonica, maclura pomifera, ulex europaeus, lens culinaris, canavalia ensiformis, phaseolus lunatus, and bandeiraea simplicifolia bs i and bs ii lectins either failed to agglutinate or agglutinated some strains but not others. agglutination of gonococci by ... | 1980 | 6155190 |
| lectins discriminate between pathogenic and nonpathogenic south american trypanosomes. | cell surface carbohydrates of trypanosoma cruzi, trypanosoma rangeli, and trypanosoma conorhini were analyzed by a micro-agglutination assay employing 27 highly purified lectins and by binding assays using various 125i-labeled lectins. the following seven lectins discriminated between the trypanosomes: 1) tomato lectin (an n-acetyl-d-glucosamine-binding protein), both in purified form and as crude tomato juice; 2) bauhinea purpurea and sophora japonica lectins (both n-acetyl-d-galactosamine-bind ... | 1984 | 6385741 |
| rabbit anti-carbohydrate antibody elicited by the lymphocyte mitogenic glycoprotein from wistaria floribunda seeds. | rabbit antibody produced in response to the purified mitogenic glycoprotein lectin from wistaria floribunda seeds (wfm) contains anti-carbohydrate antibody. this antibody, which represents 25% of the total antibody precipitated by the homologous antigen cross-reacts with the glycoprotein hemagglutinating lectins from sophora japonica (sjl), w. floribunda (wfa) and the glycoprotein bromelain, but not the protein lectin from maclura pomifera seeds. the cross-reactive reaction is totally abolished ... | 1983 | 6412071 |
| [interaction in the antibacterial activity of flavonoids from sophora japonica l. to propionibacterium]. | | 1984 | 6491872 |
| subsets of epidermal langerhans cells as defined by lectin binding profiles. | in this study we characterize the cell surface glycoconjugate moieties of strain 2 guinea pig epidermal langerhans cells (lc) in single cell suspension by using a battery of 17 fluorescent lectins. all lc displayed binding sites for concanavalin a, succinylated concanavalin a, lens culinaris agglutinin, pisum sativum agglutinin, wheat germ agglutinin, succinylated wheat germ agglutinin, griffonia simplicifolia agglutinin i, ricinus communis agglutinin i, phaseolus vulgaris e agglutinin, and phas ... | 1983 | 6631049 |
| [antimicrobial activity of a tincture of japanese pagoda tree (sophora japonica) and of the essential oil of sweet flag (acorus calamus)]. | | 1982 | 6755193 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
| identification of liver cell membrane galactoglycoproteins involved in the process of insulin binding. | the glycoproteinic nature of the insulin receptor was indicated using two different approaches: 1. [125i] insulin binding to soluble receptors from mouse liver was inhibited by digestion with beta-galactosidase or pretreatment with ricinus communis i or concanavalin a. an other enzyme (neuraminidase) and lectins (wheat germ agglutinin, dolichos biflorus) did not affect the binding reaction. these data confirmed that insulin directly interacts with the galactoglycoproteins of liver membranes. 2. ... | 1981 | 7030399 |
| inhibitory activities of substances present in plant seeds and fruits against anti-lewis agglutinins. | thirty-six species of higher plants were found to contain substances in their fruit or seed extracts, which were heat stable and inhibitory for anti-lea agglutinin. both preparations partially fractionated from sophora japonica and euonymus sieboldiana were also slightly inhibitory for anti-leb agglutinin. | 1982 | 7086155 |
| circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols. | the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ... | 1982 | 7150429 |
| binding-site specificity of lectins from bauhinia purpurea alba, sophora japonica, and wistaria floribunda. | | 1980 | 7438131 |
| lectin receptors on human blood and bone marrow cells and their use in cell separation. | a series of fluorescein-conjugated lectins (sophora japonica agglutinin, helix pomatia agglutinin, peanut agglutinin, the erythroagglutinin from phaseolus vulgaris, pokeweed mitogen, wheat germ agglutinin and the fucose-binding lectin from lotus tetragonolobus) were analyzed for their binding to human peripheral blood cells with a fluorescence-activated cell sorter. most of the lectins showed increasing cell binding in the order erythrocytes less than lymphocytes less than monocytes less than ne ... | 1980 | 7470630 |
| flow cytometric detection of bicarbonate-induced changes in lectin binding in boar and ram sperm populations. | boar and ram spermatozoa were incubated in tyrode's medium in the presence or absence of bicarbonate/co2, a component believed essential for capacitation. at intervals, samples were stained with a range of fitc-lectins to detect changes in surface glycoconjugates, using a rapid staining technique to avoid problems of lectin toxicity. the samples were then analysed directly by flow cytometry, using propidium iodide to distinguish dead cells. in the presence of bicarbonate, a live subpopulation of ... | 1995 | 7766409 |
| lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man. | the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ... | 1993 | 8310810 |
| structures and contribution to the antigenicity of oligosaccharides of japanese cedar (cryptomeria japonica) pollen allergen cry j i: relationship between the structures and antigenic epitopes of plant n-linked complex-type glycans. | the oligosaccharide structures of cry j i, a major allergenic glycoprotein of cryptomeria japonica (japanese cedar, sugi), were analysed by 400 mhz 1h-nmr and two-dimensional sugar mapping analyses. the four major fractions comprised a series of biantennary complex type n-linked oligosaccharides that share a fucose/xylose-containing core and glucosamine branches including a novel structure with a nongalactosylated fucosylglucosamine branch. rabbit polyclonal anti-cry j i igg antibodies cross-rea ... | 1996 | 8872112 |
| increase of glycocalyx and altered lectin agglutination profiles of pasteurella haemolytica a1 after incubation in bovine subcutaneous tissue chambers in vivo or in ruminant serum in vitro. | pasteurella haemolytica serotype a1 (bovine strain ok) was incubated for 2 and 6 h in bovine subcutaneous tissue chambers in vivo, and ovine strain 82-25 and bovine strain l011 were incubated in vitro for 2 h in heat-inactivated ovine or bovine serum from which gamma globulin had been depleted by protein g affinity chromatography to assess changes in morphology and lectin agglutination profiles (strains 82-25 and l101 only). cells, removed from chambers after 2 h, were covered with an extensive, ... | 1997 | 9038303 |
| analysis of lectin binding properties on human burkitt's lymphoma cell lines that show high spontaneous metastasis to distant organs in scid mice: the binding sites for soybean agglutinin lectin masked by sialylation are closely associated with metastatic lymphoma cells. | alterations in cell surface carbohydrates on human lymphoma cell lines with different spontaneous metastatic potential in the severe combined immunodeficiency (scid) mouse model were analyzed. a difference in cell surface carbohydrates between high- (hbl-2, hbl-7 and hbl-8) and no- or low-(hbl-4, hbl-6, daudi and raji) spontaneous metastatic human lymphoma cell lines were analyzed on a facscan using fluorescein-isothiocyanate (fitc)-conjugated lectins. the most consistent difference in lectin bi ... | 1996 | 9110350 |
| presence of vicia graminea lectin- and vicia unijuga lectin-binding (vgu) glycoprotein in human amniotic fluid and some of its chemical and serological properties. | vicia graminea- and vicia unijuga-binding glycoprotein (vgu glycoprotein) has been reported as a malignant tumor-associated antigen, which is found in various kinds of malignant tumor-tissues and ascitic and cyst fluids of malignant tumor patients, but not found in 20 kinds of normal human tissues. the glycoprotein reacts with anti-n lectins of vicia-graminea (vga) and vicia unijuga (vua), but does not react with anti-blood group m and n sera. the existence of vgu glycoprotein in human amniotic ... | 1997 | 9202424 |
| differential binding properties of gal/galnac specific lectins available for characterization of glycoreceptors. | differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty gal and/or galnac specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of galnac alpha 1--> ... | 1997 | 9343930 |
| core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant n-linked oligosaccharides and is present in a wide variety of plant extracts. | carbohydrates have been suggested to account for some ige cross-reactions between various plant, insect, and mollusk extracts, while some igg antibodies have been successfully raised against plant glycoproteins. a rat monoclonal antibody raised against elderberry abscission tissue (yz1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-linked immunosorbent assay against a range of plant glycoproteins and extracts as well as neoglycoprotein ... | 1998 | 9621106 |
| pollen calendar of the city of salamanca (spain). aeropalynological analysis for 1981-1982 and 1991-1992. | we report a study on the contents of airborne pollen in the city of salamanca (spain) aimed at establishing a pollen calendar for the city for the yearly periods of maximum concentrations, relating these with quantifiable atmospheric variables over two two-year periods with an interval of 10 years between them: 1981-82 and 1991-92. the pollen was captured with burkard spore-traps, based on hirst's volumetric method. determinations were made daily and were used to make preparations, previously st ... | 1998 | 9885728 |
| identification of nucleated cells in urine using lectin staining. | microscopic examination of urinary sediment is an integral component in the evaluation of nephropathies. however, identification and differentiation of the nucleated nonsquamous cells in urine is often difficult using such conventional techniques as phase contrast or bright field microscopy, even after papanicolaou staining, and requires a lot of experience. we now report a method to differentiate urinary cell types using lectin staining. twenty-five lectins were examined with respect to their b ... | 2001 | 11136172 |
| anti-inflammatory mode of isoflavone glycoside sophoricoside by inhibition of interleukin-6 and cyclooxygenase-2 in inflammatory response. | soy, high dietary intake for the oriental population, is a main source of isoflavonoids. sophoricoside (sop) an isoflavone glycoside was isolated from immature fruits of sophora japonica (leguminosae family) and its inhibitory effect on chemical mediators involved in inflammatory response was investigated in this study. sop inhibited the interleukin (il)-6 bioactivity with an ic50 value of 6.1 microm whereas it had no effects on il-1beta and tnf-alpha bioactivities. sop was identified as a selec ... | 2003 | 12735689 |
| anti-o(h) agglutinin in seeds of sophora japonica l. | | 1963 | 14062041 |
| attenuation of acute lung injury in mice by oxymatrine is associated with inhibition of phosphorylated p38 mitogen-activated protein kinase. | oxymatrine is one of the alkaloids extracted from chinese herb sophora japonica (sophora flavescens ait.) with activities of anti-inflammation, inhibiting immune reaction, antivirus, protecting hepatocytes and antihepatic fibrosis. however, the effect of oxymatrine on acute lung injury (ali) has not been known yet. in this study, the effect of oxymatrine on ali was investigated using an oleic acid-induced ali mouse model. morphological findings showed that the oleic acid group demonstrated a mar ... | 2005 | 15763380 |
| microglia, apoptosis and interleukin-1beta expression in the effect of sophora japonica l. on cerebral infarct induced by ischemia-reperfusion in rats. | sophora japonica l. (sj) is a traditional chinese herb used to cool blood, stop bleeding and to treat hemorrhoids with bleeding. although several recent studies found that both sj and ginkgo biloba have the same components of quercetin and rutin, only ginkgo biloba has been widely used to treat cerebrovascular disorders and dementia in humans. this study investigated the effect of sj on cerebral infarct in rats. a total of 66 sprague-dawley (sd) rats were studied. focal cerebral infarct was esta ... | 2005 | 16047560 |
| lectin blot studies on proteins of myxobolus cerebralis, the causative agent of whirling disease. | it is known that myxobolus cerebralis antigens, both surficial and secreted, are key modulators for, or targets of, host immune system compounds. we undertook sds-page glycoprotein characterisation of m. cerebralis developmental stages isolated from infected rainbow trout and western blot analyses using selected biotin-labelled plant lectins (gsa-i, pha-e, sja, gsa-ii) and anti-triactinomyxon polyclonal antibodies. glycoproteins were isolated with lectin-affinity chromatography, and prominent ba ... | 2005 | 16119891 |
| potential tree species for use in the restoration of unsanitary landfills. | given that they represent the most economical option for disposing of refuse, waste landfills are widespread in urban areas. however, landfills generate air and water pollution and require restoration for landscape development. a number of unsanitary waste landfills have caused severe environmental problems in developing countries. this study aimed to investigate the colonization status of different tree species on waste landfills to assess their potential for restoring unsanitary landfills in s ... | 2005 | 16132448 |
| differential assimilation of nitrogen dioxide by 70 taxa of roadside trees at an urban pollution level. | in order to screen for the best species for mitigating nitrogen dioxide (no2) by plants at urban levels, we investigated assimilation of nitrogen dioxide by 70 taxa of woody plants that are mostly utilized as roadside trees. they were fumigated with 15n-labeled no2 at 0.1 microl l(-1) for 8h, and the amount of reduced nitrogen derived from no2 (in mg ng(-1) dry weight) in the leaves (designated no2 assimilation capability hereafter) were determined. data were analyzed in the comparison with the ... | 2005 | 16219499 |
| metal bioaccumulation in plant leaves from an industrious area and the botanical garden in beijing. | the concentrations of fe, mn, al, zn, pb, ni, cr, and as were measured in soils and leaves from 21 plant species growing on hills near the beijing steel factory (bsf) and 17 plant species in the beijing botanical garden (bbg). the results showed that soils from bsf were zn contaminated according to the threshold of natural background of china. there was a metal contamination of the soils by ni, and cr in bsf comparing with those in bbg. the comparison between concentrations of metals in leaves f ... | 2005 | 16295909 |
| parental use of the term "hot qi" to describe symptoms in their children in hong kong: a cross sectional survey "hot qi" in children. | the chinese term "hot qi" is often used by parents to describe symptoms in their children. the current study was carried out to estimate the prevalence of using the chinese term "hot qi" to describe symptoms in children by their parents and the symptomatology of "hot qi". | 2006 | 16393344 |
| cosmetic applications of selected traditional chinese herbal medicines. | because tyrosinase catalyzes melanin synthesis, tyrosinase inhibitors are important in cosmetic skin-whitening. oxidative stress contributes to skin aging and can adversely affect skin health, which means antioxidants active in skin cells may support skin health. we examined 25 traditional chinese herbal medicines that might be useful for skin-whitening and skin health. extracts (100microg/ml) were tested for cytotoxicity on human epidermal melanocytes (hemn); 12 exhibited low cytotoxicity. thei ... | 2006 | 16497459 |
| specificity of aspartate aminotransferases from leguminous plants for 4-substituted glutamic acids. | aspartate aminotransferase (glutamate-oxalacetate transaminase) was partially purified from extracts of germinating seeds of peanut (arachis hypogaea), honey locust (gleditsia triacanthos), soybean (glycine max), and sophora japonica. the ability of these enzyme preparations, as well as aspartate aminotransferase purified from pig heart cytosol, to use 4-substituted glutamic acids as amino group donors and their corresponding 2-oxo acids as amino group acceptors in the aminotransferase reaction ... | 1989 | 16666674 |
| lead and cadmium in leaves of deciduous trees in beijing, china: development of a metal accumulation index (mai). | lead and cadmium uptake was investigated for common deciduous street trees in beijing in this study. species having cd accumulation included populus tomentosa, sophora japonica and catalpa speciosa. p. tomentosa had the highest ratios between leaf and soil cd (0.848), followed by s. japonica (0.536), c. speciosa (0.493), paulownia tomentosa (0.453) and juglans regia (0.415). pb levels were high in leaves of c. speciosa, j. regia and pa. tomentosa. s. japonica had the highest ratio between leaf p ... | 2007 | 16797111 |
| [anti-hiv-1 effect of compound k3 from flower of japanese pagoda tree in vitro]. | to investigate the anti-hiv properties of compound k3 purified from japanese pagoda tree flower as well as its anti-hiv mechanism in vitro. | 2006 | 16913492 |
| antihyperglycemic activity of herb extracts on streptozotocin-induced diabetic rats. | we investigated the effects of herb extracts, rhus verniciflua, agrimonia pilosa, sophora japonica, and paeonia suffruticosa, on the lowering of blood glucose levels and thiobarbituric acid reactive substances (tbars) in streptozotocin (stz)-induced diabetic rats. after 4 weeks, oral administration of rhus verniciflua extract (50 mg/kg) exhibited a significant decrease in blood glucose levels in diabetic rats (p<0.05). blood tbars concentrations, the products of glucose oxidation in blood, were ... | 2006 | 17031059 |
| pharmacological studies of the large-scaled purified genistein from huaijiao (sophora japonica-leguminosae) on anti-osteoporosis. | in this report, we used genistein that was extracted from a chinese herbal medicine huaijiao (sophora japonica-leguminosae) to evaluate its pharmacological function on anti-osteoporosis. this genistein is purified in a large-scale production from huaijiao by a state-of-art method as described by tian et al. [2004. the preparation of genistein and lc-ms/ms on-line analysis. drug devel. res. 61, 6-12]. chemical structure of the isolated genistein was examined by using various techniques including ... | 2006 | 17085294 |
| [morphological-ecological characters and growth patterns of main tree species leaves in urban forest of shenyang]. | the study with statistic and multivariate analyses showed that the main meteorological factors affecting the growth and development rhythms of main tree species leaves in urban forest of shenyang were > or = 5 degrees c accumulated temperature, accumulated sunshine hours, and mean temperature in the middle ten days of each phenological period. the meteorological factors needed by the tree species varied with their phenological period. necessary low temperature and ci were required in germination ... | 2006 | 17269315 |
| physicochemical characteristics of ambient particles settling upon leaf surfaces of urban plants in beijing. | particulate pollution is a serious health problem throughout the world, exacerbating a wide range of respiratory and vascular illnesses in urban areas. urban plants play an important role in reducing particulate pollution. physicochemical characteristics of ambient particles settling upon leaf surfaces of eleven roadside plants at four sites of beijing were studies. results showed that density of particles on the leaf surfaces greatly varied with plant species and traffic condition. fraxinus chi ... | 2006 | 17278748 |
| characterization of glycans in the developmental stages of myxobolus cerebralis (myxozoa), the causative agent of whirling disease. | glycans and sugar-binding molecules (lectins) form an interactive recognition system, which may enable parasitic organisms to adhere to host cells and migrate into target tissues. the aim of the present study was to analyse surface-associated glycans in the developmental stages of myxobolus cerebralis (hofer), the causative agent of whirling disease. a panel of biotin-labelled plant lectins was used to detect a broad spectrum of glycan motifs with high specificity. binding sites were detected hi ... | 2007 | 17958607 |
| attenuation of bleomycin-induced lung fibrosis by oxymatrine is associated with regulation of fibroblast proliferation and collagen production in primary culture. | there is no satisfactory treatment for pulmonary fibrosis, which is characterized by altered control of proliferation of mesenchymal fibroblasts and extracellular matrix production. oxymatrine is an alkaloid extracted from the chinese herb sophora japonica (sophora flavescens ait.) with capacities of anti-inflammation, inhibition of immune reaction, antivirus, protection against acute lung injury and antihepatic fibrosis. in this study, the effect of oxymatrine on pulmonary fibrosis was investig ... | 2008 | 18684219 |
| anti-platelet effects of flavonoids and flavonoid-glycosides from sophora japonica. | a methanol extract of sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin a (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). the structure of each compound was determined by a variety of spectroscopic methods. among the compounds, 1, 3, and 7 showed approximately 2.5-6.5 fold greater inhibit ... | 2008 | 18704331 |
| active constituents from sophora japonica exhibiting cellular tyrosinase inhibition in human epidermal melanocytes. | there is greater consumer awareness of plant-based skin-care products. sophora japonica l. (fabaceae) has been used traditionally as a hemostatic agent and also has skin-care and whitening benefits. the effect of the isolated active compounds of sophora japonica l. (fabaceae) that inhibits tyrosinase activity in human epidermal melanocytes (hemn) was examined. | 2009 | 19422905 |
| lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus beauveria bassiana. | the entomopathogenic fungus beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. b. bassiana produces a number of distinct cell types that include aerial conidia, submerged conidia, blastospores and haemolymph-derived cells termed in vivo blastos ... | 2009 | 19608611 |
| diets containing sophora japonica l. prevent weight gain in high-fat diet-induced obese mice. | obesity, a worldwide epidemic, is associated with metabolic diseases such as insulin resistance, dyslipidemia, hypertension, and heart disease. many strategies, including natural alternative antiobesity agents, have been widely used to prevent obesity. polyphenolic compounds and flavonoids from natural products are shown to inhibit adipogenesis. because mature fruits of sophora japonica l. were previously shown to contain antiadipogenic compounds, we hypothesized that diets with mature fruits of ... | 2009 | 19932871 |
| paenibacillus sophorae sp. nov., a nitrogen-fixing species isolated from the rhizosphere of sophora japonica. | a nitrogen-fixing bacterium, designated strain s27(t), was isolated from rhizosphere soil of sophora japonica. phylogenetic analysis based on a fragment of the nifh gene and the full-length 16s rrna gene sequence revealed that strain s27(t) is a member of the genus paenibacillus. high levels of 16s rrna gene sequence similarity were found between strain s27(t) and paenibacillus durus dsm 1735(t) (97.3 %), paenibacillus sabinae dsm 17841(t) (96.9 %), paenibacillus forsythiae dsm 17842(t) (96.7 %) ... | 2011 | 20435753 |
| a new isoflavone glycoside from the stem bark of sophora japonica. | a new isoflavone glycoside, 6-methoxy-7-hydroxy-4'-o-beta-d-glucosyl isoflavone, glycitein-4'-o-beta-d-glucoside (10), along with nine known flavonoids, were isolated from the stem bark of sophora japonica. the structures of these compounds were determined by analysis of spectroscopic data (1d -, 2d - nmr and hrms). the inhibitory effects of all the isolated compounds on aldose reductase were evaluated in vitro. among these compounds, daidzein (1), puerol a (4), and paratensein-7-o-glucoside (9) ... | 2010 | 20803118 |
| effects of sophora japonica flowers (huaihua) on cerebral infarction. | abstract: the dried flowers and buds of sophora japonica are used as a medicinal herb in china, japan and korea to treat bleeding hemorrhoids and hematemesis. this article presents an overview of the effects of sophora japonica on cerebral infarction based on literature searched from medline, pubmed, cochrane library and the china national knowledge infrastructure (cnki). sophora japonica contains both anti-hemorrhagic and anti-hemostatic substances. sophora japonica reduces cerebral infarction ... | 2010 | 20875105 |
| genistein mediates the anti-adipogenic actions of sophora japonica l. extracts. | previous studies showed that feeding diets containing the mature fruits of sophora japonica l. prevented body weight gain and reduced fat mass in high-fat diet-induced obese mice. this observation has led to the hypothesis that extracts from s. japonica l. may inhibit adipocyte differentiation of preadipocytes. to elucidate the possible mechanisms for the anti-obesity action of s. japonica l., its effects on adipocyte differentiation were investigated in c3h10t1/2 mesenchymal stem cells and 3t3- ... | 2011 | 21303259 |
| protective effect of oxymatrine on myocardial fibrosis induced by acute myocardial infarction in rats involved in tgf-ß1-smads signal pathway. | oxymatrine (1), a component extracted from a traditional chinese herb sophora japonica (sophora flavescens ait.), has been demonstrated to have a variety of pharmacological actions. abundant experimental evidence indicates that 1 may exert a protective effect on the cardiovascular system. this study was designed to explore the possible role of 1 against myocardial fibrosis induced by acute myocardial infarction (ami) and its modulation on transforming growth factor beta 1 (tgf-ß(1))-smads signal ... | 2011 | 21409682 |
| a review on the dietary flavonoid kaempferol. | epidemiological studies have revealed that a diet rich in plant-derived foods has a protective effect on human health. identifying bioactive dietary constituents is an active area of scientific investigation that may lead to new drug discovery. kaempferol (3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-1-benzopyran-4-one) is a flavonoid found in many edible plants (e.g. tea, broccoli, cabbage, kale, beans, endive, leek, tomato, strawberries and grapes) and in plants or botanical products commonly used ... | 2011 | 21428901 |
| bacterial adherence in otitis media: determination of n-acetylgalactosamine (galnac) residues in the submucosal glands and surface epithelium of the normal and diseased eustachian tube. | acute otitis media (aom) is the most common childhood infection caused by bacteria. the pathogenesis of aom implicates initial adherence of a pathogen to the nasopharyngeal epithelium, which is followed by bacterial colonization of the middle ear cavity through the eustachian tube. n-acetylgalactosamine (galnac) is an important constituent of mucins and galnac containing sugar residues seem to be essential for initial adherence of respiratory bacteria to the surface of epithelial cells. | 2011 | 21440051 |
| screening for estrogenic and antiestrogenic activities of plants growing in egypt and thailand. | there is a growing demand for the discovery of new phytoestrogens to be used as a safe and effective hormonal replacement therapy. | 2011 | 21772754 |
| protective effect of oxymatrine on chronic rat heart failure. | oxymatrine is one of the alkaloids extracted from the chinese herb sophora japonica (sophora flavescens ait.) with anti-inflammatory, immune reaction inhibiting, antiviral, and hepatocyte and antihepatic fibrosis protective activities. however, the effect of oxymatrine on heart failure is not yet known. in this study, the effect of oxymatrine on heart failure was investigated using a sprague-dawley rat model of chronic heart failure. morphological findings showed that in the group treated with 5 ... | 2011 | 21691940 |
| Antioxidant and Anti-inflammatory Activities of Selected Medicinal Plants Containing Phenolic and Flavonoid Compounds. | The antioxidant, anti-inflammatory, and cytotoxic activities of water and ethanol extracts of 14 Chinese medicinal plants were investigated and also their total phenolics and flavonoid contents measured. The antioxidant activity was evaluated in a biological assay using Saccharomyces cerevisiae , whereas the radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolics and flavonoid contents were estimated by Folin-Ciocalteu and aluminum chlorid ... | 2011 | 22023309 |
| the separation of lymphocyte subpopulations with lectins. | wistaria floribunda agglutinin (wfa), sophora japonica agglutinin (sja) and maclura pomifera lectin (mpl) were employed as immunofluorescent and leucoagglutinating reagents to study murine lymphocytes. wfa, which labels 90% of thymocytes, binds to only 57% of the splenocyte population. the latter subset corresponds to surface immunoglobulin bearing cells. differential agglutination of splenocytes with this lectin results in the isolation of a wfa negative population which exhibits t-lymphocyte s ... | 2013 | 3793157 |
| hapten inhibition of adsorption: specificity of the sophora japonica lectin. | since hapten inhibition of precipitation is relatively time consuming, we have developed a hapten inhibition of adsorption assay to explore the sugar binding specificity of the sophora japonica lectin. adsorbents were prepared with sephadex and p-aminophenyl beta-d-galactopyranoside using the cnbr procedure. the ability of simple saccharides to inhibit the binding of lectin to the adsorbent was performed employing a fixed amount of adsorption with respect to the logarithm of micromoles of inhibi ... | 1986 | 239067 |
| k88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. | the formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic escherichia coli k88 (e. coli k88) was investigated. glycated albumin with lactose (bsa-glucose-β (4-1) galactose) was used as the microsphere matrix (ms-lac) and gentamicin included as the transported antibiotic. the proposed target strategy was that exposed galactoses of ms-lac could be specifically recognized by e. coli k88 adhesins, and the delivery of gentamicin would inhibi ... | 2015 | 26389896 |
| sympodiomycopsis yantaiensis sp. nov., a basidiomycetous yeast isolated from insect frass. | two strains (nynu 121010(t) and nynu 121032) of a novel basidiomycetous yeast species belonging to the genus sympodiomycopsis were isolated from insect frass collected from trunks of a pagoda tree (sophora japonica l.) in yantai, shandong province, east china. the sequence analyses of the d1/d2 domain of the large subunit (lsu) rrna gene and the internal transcribed spacer (its) region indicated that the closest relatives were sympodiomycopsis kandeliae firdi 007(t), sympodiomycopsis paphiopedil ... | 2013 | 23838443 |
| flavonoid glycosides inhibit sortase a and sortase a-mediated aggregation of streptococcus mutans, an oral bacterium responsible for human dental caries. | three flavonoids were isolated from dried flowers of sophora japonica using repetitive column chromatography and high-performance liquid chromatography. the flavonoids were identified as rutin (1), quercetin-3'-o-methyl-3-o-α-l-rhamnopyranosyl(1 → 6)-β-d-glucopyranoside (2), and quercetin (3) on the basis of spectroscopic analysis and comparison of values reported in the literature. these compounds inhibited the action of sortase a (srta) from streptococcus mutans, a primary etiologic agent of h ... | 2016 | 27291675 |
| streptococcus mutans sortase a inhibitory metabolites from the flowers of sophora japonica. | a new maltol derivative (2) along with three known maltol derivative (1) and flavonol glycosides (3 and 4) were isolated from the dried flowers of sophora japonica. based upon the results of combined spectroscopic methods, the structure of new compound (2) was determined to be maltol-3-o-(4'-o-cis-p-coumaroyl-6'-o-(3-hydroxy-3-methylglutaroyl))-β-glucopyranoside, an isomer of 1. these compounds strongly inhibited the action of sortase a (srta) from streptococcus mutans, a primary etiologic agent ... | 2015 | 25746812 |
| relationship between galactomannan structure and physicochemical properties of films produced thereof. | in this work five sources of galactomannans, adenanthera pavonina, cyamopsis tetragonolobus, caesalpinia pulcherrima, ceratonia siliqua and sophora japonica, presenting mannose/galactose ratios of 1.3, 1.7, 2.9, 3.4 and 5.6, respectively, were used to produce galactomannan-based films. these films were characterized in terms of: water vapour, oxygen and carbon dioxide permeabilities (wvp, o 2 p and co 2 p); moisture content, water solubility, contact angle, elongation-at-break (eb), tensile stre ... | 2015 | 26604406 |
| pollen analysis of natural honeys from the central region of shanxi, north china. | based on qualitative and quantitative melissopalynological analyses, 19 chinese honeys were classified by botanical origin to determine their floral sources. the honey samples were collected during 2010-2011 from the central region of shanxi province, north china. a diverse spectrum of 61 pollen types from 37 families was identified. fourteen samples were classified as unifloral, whereas the remaining samples were multifloral. bee-favoured families (occurring in more than 50% of the samples) inc ... | 2012 | 23185358 |
| [effects of nacl stress on the seedling growth and k(+)- and na(+) -allocation of four leguminous tree species]. | taking the pot-cultured seedlings of four leguminous tree species (albizia julibrissin, robinia pseudoacacia, sophora japonica, and gleditsia sinensis) as test materials, this paper studied their growth indices, critical salt concentration (c50), and k+ and na+ allocation under different levels of nacl stress, aimed to understand the difference of test tree species in salt tolerance. nacl stress inhibited the seedling growth of the tree species. under nacl stress, the dry matter accumulation dec ... | 2011 | 21812288 |
| kaempferol, a potential cytostatic and cure for inflammatory disorders. | kaempferol (3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-1-benzopyran-4-one) is a flavonoid found in many edible plants (e.g., tea, broccoli, cabbage, kale, beans, endive, leek, tomato, strawberries, and grapes) and in plants or botanical products commonly used in traditional medicine (e.g., ginkgo biloba, tilia spp, equisetum spp, moringa oleifera, sophora japonica and propolis). its anti-oxidant/anti-inflammatory effects have been demonstrated in various disease models, including those for encephal ... | 2014 | 25147152 |
| differential binding characteristics and applications of dgal beta 1----3dgalnac specific lectins. | the binding properties of arachis hypogaea (pna), bauhinia purpurea alba (bpl), maclura pomifera ( mpl ) and sophora japonica (sjl) lectins were studied by quantitative precipitin and precipitin inhibition assays, demonstrating them to be most specific for dgal beta 1---- 3dgalnac residues. additionally, each lectin had its own binding characteristic such as different binding activities to dgal beta 1---- 4dglcnac or dgal beta 1---- 3dglcnac beta 1----linked oligosaccharides, and/or dgalnac alph ... | 1984 | 6727869 |
| histochemical studies on lectin binding in reactive lymphoid tissues. | using the avidin-biotin-labeled peroxidase complex (abc) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with concanavalin ensiformis (con a), lens culinaris (lca), phaseolus vulgaris (pha), pisum sativum (psa), ricinus communis (rca), and triticum vulgaris (wga) agglutinins, but no evidence of bindin ... | 1983 | 6827084 |
| detection of lectin-sugar interaction by ultraviolet difference spectroscopy. | it was found that the addition of specific sugars to solutions of several lectins induced ultraviolet difference spectra. the difference spectra of lectins from lens culinaris, sophora japonica, solanum tuberosum and wheat germ have two peaks at 292 nm and 284-287 nm which are characteristic of the tryptophanyl residue. the difference spectrum of arachis hypogaea agglutinin has two peaks at 285 nm and 279 nm which seem to be characteristic of the tyrosyl residue. in addition to the identificatio ... | 1980 | 7451408 |
| solid-phase assay of lectin activity using hrp-conjugated glycoproteins. | various enzyme-conjugated probes have been widely used for detection of specific interactions between biomolecules. in the case of glycan-protein interaction, horseradish peroxidase (hrp)-conjugated glycoproteins (hrp-gps) are useful for the detection of carbohydrate-binding activity of plant and animal lectins. in this chapter, a typical solid-phase assay of the carbohydrate-binding activity of sophora japonica agglutinin i, a gal/galnac-specific lectin, using hrp-conjugated asialofetuin is des ... | 2014 | 25117228 |
| a novel mannose-specific and sugar specifically aggregatable lectin from the bark of the japanese pagoda tree (sophora japonica). | a new d-mannose/d-glucose-specific lectin (b-sja-ii) was isolated from the bark of the japanese pagoda tree, sophora japonica. b-sja-ii was separated from a well known d-galactose/n-acetyl-d-galactosamine-specific lectin (b-sja-i) by affinity chromatography on lactamyl-sepharose, then purified by affinity chromatography on maltamyl-sepharose. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, b-sja-ii gave four bands: subunit a-1 (mr = 19,400), a-2 (mr = 18,200), b-1 (mr = 15,000), an ... | 1991 | 1993686 |