| the role of divalent cations in the activation of the nadp+-specific isocitrate dehydrogenase from pisum sativum l. | a divalent cation electrode was used to measure the stability constants (association constants) for the magnesium and manganese complexes of the substrates for the nadp+-specific isocitrate dehydrogenase (ec 1.1.1.42) from pea stems. at an ionic strength of 26.5 mm and at ph 7.4 the stability constants for the mg2+-isocitrate and mg2+-nadp+ complexes were 0.85 +/- 0.2 and 0.43 +/- 0.04 mm-1 respectively and for the mn2+-isocitrate and mn2+-nadp+ complexes they were 1.25 +/- 0.07 and 0.75 +/- 0.0 ... | 1977 | 20207 |
| strain-specific antigens in rhizobium leguminosarum. | fifty strains of rhizobium leguminosarum, isolated from five species of host plant (pisum sativum, p. arvense, vicia sativa, v. faba, and a lathyrus sp.) were examined for the presence of strain-specific somatic antigens by immune-diffusions against 13 antisera. thirty eight strains (76 per cent) were found to belong to the same sero-group and were serologically indistinguishable from each other, but four of these strains also exhibited non-reciprocal cross reactivity with other antisera. in con ... | 1979 | 89766 |
| a comparison of dna from free living and endosymbiotic rhizobium leguminosarum (strain pre). | 1. bacteroids of rhizobium leguminosarum (strain pre) purified from root nodules of pisum sativum (var. 'rondo') by the standard procedure of differential centrifugation contained considerable contamination of mitochondrial material. this could be removed by incubation of the bacteroid preparation with 1 m kcl/1% deoxycholate. 2. the dna content of bacteroid cells of r. leguminosarum was found to have increased about three fold in comparison with the dna content of free living r. leguminosarum b ... | 1975 | 172157 |
| midpoint redox potentials of plant and algal ferredoxins. | midpoint potentials of plant-type ferredoxins from a range of sources were measured by redox titrations combined with electron-paramagnetic-resonance spectroscopy. for ferredoxins from higher plants, green algae and most red algae, the midpoint potentials (at ph 8.0) were between --390 and --425 mv. values for the major ferredoxin fractions from blue-green algae were less negative (between --325 and --390 mv). in addition, spirulina maxima and nostoc strain mac contain second minor ferredoxin co ... | 1977 | 202262 |
| [redox potentials of some metalloproteins]. | the standard redox potentials of soluble cytochromes c isolated from the green alga chlorella and the blue-green algae spirulina and aphanezomenon were determined by potentiometric titration and found to be equal to +380 mb, +330 mb and +357 ab, respectively. the standard redox potentials of plastocyanin preparations from pisum sativum and atriplex leaves were also determined and found close to those of soluble cytochromes c, i. e. +395 mb and +375 mb, respectively. the metalloproteins studied w ... | 1978 | 207361 |
| purification and characterization of two cellulases from auxin-treated pea epicotyls. | two forms of beta-1,4-glucan 4-glucanohydrolase (ec 3.2.1.4) were extracted from growing regions of pisum sativum epicotyls which had been treated with the auxin, (2,4-dichlorophenoxy)acetic acid. one cellulase is buffer-soluble, the other buffer-insoluble but extractable with high salt concentrations. both enzymes catalyze endohydrolysis of carboxymethylcellulose with the same ph optimum (5.5 to 6.0). they were purified with the use of deae-cellulose chromatography, sephadex gel filtration, and ... | 1975 | 234438 |
| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| [cloning chloroplast dna in escherichia coli. i. construction and selection of recombinant plasmids containing fragments of pea chloroplast dna]. | fragments produced by digestion of pisum sativum chloroplast dna with ecori were examined by agarose gel electrophoresis. these ecori-fragments were joined in vitro to apr-cole1 rsf2124 plasmid and cloned in escherichia coli. methods of molecular cloning of plasmid chimeras by success gradient centrifugation and repeated transformation and selection of recombinant plasmids using mytomicin c were used for cloning hybrid plasmids with various ecori fragments of pea chloroplast dna has been obtaine ... | 1978 | 363514 |
| [aflatoxins produced by aspergillus flavus in soya and other legumes]. | there is no doubt that our programme of applied nutrition must include soya on account of the high nutritive value of the legume. this underlines research undertaken here regarding the risks of contamination by a. flavus and the subsequent formation of aflatoxins on and in soya beans. we have studied on parallel lines soya beans and other legumes important in the local dietary habits. these legumes are either obtained locally or imported. on every specimen of legume we have tried to confirm, or ... | 1977 | 418721 |
| development of the nitrogen-fixing and protein-synthesizing apparatus of bacteroids in pea root nodules. | some aspects of root nodule development of pisum sativum inoculated with rhizobium leguminosarum were examined. 1. nitrogenase activity (measured as acetylene reduction) appears to be preceded by leghemoglobin synthesis (measured immunologically). 2. syntheses of component i and component ii of nitrogenase are not strictly coordinated. synthesis of component i starts before component ii. 3. plant and bacteroid protein synthesis (measured by [35s]sulfate labeling) in root nodules declines rapidly ... | 1979 | 454614 |
| different manifestations of the pathogenity of some strains of fusarium oxysporum f. sp. pisi. | different cultivation and morphology characteristics were found in 10 monospore isolates of fusarium oxysporum f. sp. pisi, obtained from yellowing and wilting plants of pea (pisum sativum l.). the isolates of the fungus were obtained from distant geographical regions of czechoslovakia and from various cultivars and hybrids of pea. after inoculation of roots, followed by constant conditions of incubation of the meteor and jupiter cultivars having their origin at the plant-breeding station at luz ... | 1979 | 473967 |
| 1,3-beta-d-glucanases from pisum sativum seedlings. ii. substrate specificities and enzymic action patterns. | two purified pea 1,3-beta-d-glucanases (ec 3.2.1.6) hydrolyse laminarin (degree of polymerization 20), laminaridextrins (degree of polymerization 3--7), and their reduced 3h-derivatives, 1,3-beta-d-glucans which are partially substituted (carboxymethyl-pachyman) or crystalline (curdlan), and mixed-linkage beta-glucans. enzyme kinetics and product-formation indicate endo-hydrolase activity with weak transglycosylase capacity. the enzymes do not hydrolyse beta-glucosides, the 1,3 linkage adjacent ... | 1979 | 508768 |
| lectin-associated proteins from the seeds of leguminosae. | the seeds of pisum sativum (pea), canavalia ensiformis, vicia faba, vicia sativa, and ricinus communis were shown to contain proteins which are associated to the respective lectins (lectin binders). the lectin binders from pisum sativum and canavalia ensiformis were studied more closely. both are single proteins not resembling the variety of membrane glycoproteins found in animals and plants which bind to lectins. the pea lectin binder is a tetrameric glycoprotein composed of identical subunits ... | 1979 | 511093 |
| changes in chlorophyll and primary productivity of pea (pisum sativum l.) leaves as influenced by arhar (pigeon pea) mosaic virus infection. | | 1979 | 554306 |
| the binding of lectins to components of plasma membranes from porcine submaxillary lymph node lymphocytes. | by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis the plasma membranes from porcine lymphocytes contain at least 30--35 glycopolypeptides and one or more glycolipids to which one or more of 12 purified lectins bind. the specificities of binding generally followed the same pattern as those of the reaction of the lectin with intact pig lymphocytes. some lectins (e.g., the isolectin pair, agaricus bisporus lectins a and b and a group consisting of the lens culinaris a and b isol ... | 1978 | 568485 |
| tissue selenium and serum tocopherol concentrations in selenium-vitamin e deficient pigs fed peas (pisum sativum). | | 1977 | 606705 |
| pea enation mosaic virus genoma rna contains no polyadenylate sequences and cannot be aminoacylated. | an active synthetase enzyme preparation from peas (pisum sativum l.) did not catalyze the aminoacylation of pea enation mosaic virus rna. the viral rna was shown not to contain polyadenylic acid sequences. | 1978 | 618834 |
| the effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by rhizobium leguminosarum. | the effects of nh4no3 on the development of root nodules of pisum sativum after infection with rhizobium leguminosarum (strain pre) and on the nitrogenase activity of the bacteroids in the nodule tissue were studied. the addition of nh4no3 decreased the nitrogenase activity measured on intact nodules. this reduction of nitrogen fixation did not result from a reduced number of bacteroids or a decreased amount of bacteroid proteins per gram of nodule. the synthesis of nitrogenase, measured as the ... | 1978 | 623788 |
| influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (pisum sativum). | a study was made on the short-term effect of ammonium ions on the nitrogenase activity of pea root nodules. nodulated pea plants (pisum sativum), having reached maximum acetylene-reducing activity, were supplied with nh4cl (20 mm). nitrogenase activity of intact plants, detached nodules, and isolated bacteroids was measured at differed time intervals. a significant drop (20 to 40%) in the acetylene-reducing activity of treated intact plants and their detached nodules was observed after 1 day. no ... | 1978 | 677873 |
| trifluralin effect on pisum--rhizobium relationship. | trifluralin inhibited root and shoot elongation of pisum sativum plant and caused isodiametric increase in cell volume of both tissues. the water content of the plant was not affected. the weedicide inhibited also growth and o2 uptake of rhizobium leguminosarum, isolated from pisum plant. reduction in the nitrogen content of pisum tissues was noticed, and this may be attributed to the inhibition of nodule formation. variations in the free and protein-amino acids were observed in the plant tissue ... | 1978 | 726711 |
| dna sequence organization in the pea genome. | the reassociation kinetics of pea (pisum sativum l.) dna fragments (300 nucleotides) were measured with hydroxylapatite. the most slowly reassociating fragments do so with a rate constant of 2 x 10(-4) l mol-1s-1, as determined from experiments with total dna as well as with a tracer enriched for slowly renaturing sequences. this rate is about 1000 times slower than that observed for escherichia coli dna included as an internal kinetic standard, indicating a kinetic complexity of 4.5 x 10(9) nuc ... | 1978 | 728436 |
| [kinetic study of human lymphocytes transformation in culture by lectin from pisum sativum (author's transl)]. | some vegetable extract have haemagglutinating activity and are able to transform lymphocytes in culture. the comparative activities of lectin from pisum sativum and other lectins already used (phytohaere shown. this lectin can be isolated in a highly purified form which is described. purification of the human blood lymphocytes is obtained through nylon columns and measurement of transformation and proliferation by means of 3 h thymidine incorporation. normal lymphocyte stimulation can be obtaine ... | 1976 | 790278 |
| histochemical lectin affinity technique by means of fitc-labeled serum protein fractions. | a two-step affinity technique is described for light microscopic demonstration of the concanavalin a, agaricus bisporus lectin and ricinus communis lectin binding sites by means of various fitc-labeled human and rabbit serum protein fractions. experiments for the visualization of the lens culinaris lectin and the pisum sativum lectin binding sites gaves negative results. the technique consist of two reaction steps which involve the incubation of tissue sections in the lectins followed by the vis ... | 1976 | 791903 |
| structural repeating units in chromatin. iii. a comparison of chromatin subunits from vertebrate, cilliate and angiosperm species. | a comparison was made of the chromatin subunit ("nu"body) structure present in nuclei from chicken erythrocytes, tetrahymena cells, and pisum sativum (pea) buds. all three types of chromatin yielded spherical subunits upon nuclease digestion which were indistinguishable in the electron microscope, and contained approximately the same amount of dna. there were, however, consistent and significant differences in the digestion patterns of chromatin from the three organisms. | 1977 | 851537 |
| delayed conversion of squalene to sterols during development of pinus pinea seeds. | during germination of seeds of the gymnosperm, pinus pinea, radioactivity from [2-14c]-mevalonate proceeded principally through the anaerobic reactions leading to squalene in the first 24 hr in both the haploid endosperm and the diploid embryo, and only with succeeding time (3-9 days) in both cases was label transferred to sterols in oxygen-requiring steps. the rates of turnover must be real and independent in the two tissues, since no consequential interchange of labelled lipids occurred betwee ... | 1977 | 853880 |
| attempts to overcome anti-nutritive factors in field beans (vicia faba l) and field peas (pisum sativum) fed in diets to laying hens. | | 1977 | 905287 |
| citric acid cycle enzymes and nitrogenase in nodules of pisum sativum. | activity of isocitric dehydrogenase (isocitrate dehydrogenase (nadp+); ec 1.1.1.42) in bacteroids is highest at the time of maximum nitrogen fixation. it is likely that isocitric dehydrogenase is the source of reductant for dinitrogen fixation. | 1977 | 907916 |
| on the origin of plastids. | the buoyant density in cscl of ribosomes from chloroplasts of the green alga chlorella pyrenoidosa and two species of higher plants, pisum sativum and chenopodium album, has been studied. from the relative protein content it was calculated that 70s ribosomes from chloroplasts are much smaller than 80s cytoplasmic ribosomes (3.0-3.1 x 10(6) and 4.0 x 10(6) daltons) and slightly larger than 70s ribosomes from bacteria (e. coli 2.5 x 10(6) daltons). chloroplast ribosomes from pea seedlings were ana ... | 1975 | 1103046 |
| selenium-vitamin e deficiency in swine fed peas (pisum sativum). | an experiment was conducted to study the effects of feeding a 96.8% cull pea basal ration, low in selenium (0.061 ppm) and vitamin e (7.0 iu alpha-tocopherol/kg of ration), to growing pigs with and without supplementation of selenium, vitamin e, or both. the basal ration was high in crude protein (25.2%) and contained no supplemented fat. nine of 10 pigs fed the unsupplemented basal ration had lesions attributed to selenium-vitamin e deficiency, and 8 of these pigs died during the 160-day experi ... | 1975 | 1115425 |
| carbohydrate binding specificity of the lectin from the pea (pisum sativum). | hapten inhibition measurements on the precipitin reaction between pisum sativum lectin and pichia pinus phosphomannan showed the lectin to bind d-mannose, d-glucose, d-fructose and l-sorbose. unmodified hydroxyl groups at the c-4 and the c-6 positions of the d-glucopyranose ring were essential for binding to the protein. modification of the c-2 hydroxyl group was allowed in the d-glucopyranose ring but not in the d-mannopyranose configuration. substitution of the hydroxyl hydrogen atom at the c- ... | 1975 | 1122297 |
| an evolutionary comparison of plant histones. | histones were extracted from chromatin of the following: a moss (polytrichum juniperinum); the primitive vascular plants psilotum nudum and equisetum arvense; a fern (polypodium vulgare); the gymnosperms fir (abies concolor), yew (taxus canadensis) and gingko biloba; the dicotyledonous angiosperms tobacco (nicotiana tabacum) and maple (acer saccharinum); and the monocotyledonous angiosperms corn (zea mays) and lily (lilium longiflorum). the histones were subjected to polyacrylamide gel electroph ... | 1975 | 1164518 |
| observations with cytochemistry and ultracryotomy on the fine structure of the expanding walls in actively elongating plant cells. | ultracryotomy with negative staining and cytochemistry (periodic acid - thiocarbohydrazide - silver proteinate test for polysaccharides, in conjunction with mild extractions) were used to study the architecture of the cell wall and its modifications during expansion. those techniques were applied to the study in situ of the walls of actively elongating parenchyma of mung bean (phaseolus aureus), and pea (pisum sativum) root and of collenchyma of celery (apium graveolens) petioles. these compleme ... | 1975 | 1202041 |
| changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding. | lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) c57 bl/6j mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. the following lectins were used: canavalia ensiformis con a, triticum vulgaris (wga), glycine max (sba), griffonia simplicifolia (gs ii), arachis hypogaea (pna), pisum sativum (psa) and lens culinaris (lca). after incubation of frozen sections with con a, wga, gs ii, psa and lca a sarcoplasmic staining was obse ... | 1992 | 1290587 |
| [study of the pisum sativum agglutinin receptors and dna ploidy during carcinogenesis in esophageal epithelium by flow cytometry]. | the relative mean contents of pisum sativum agglutinin receptors and dna of human normal esophageal epithelium, its atypical hyperplasia as well as esophageal carcinoma were determined respectively by flow cytometry. the results revealed that the relative mean contents of pisum sativum agglutinin receptors which denote membrane composition and dna which denote nuclear composition were increased gradually from normal, dysplastic and then malignant esophageal tissues. this suggested that during th ... | 1992 | 1299528 |
| molecular characterization of glutathione reductase cdnas from pea (pisum sativum l.). | a cdna for pea glutathione reductase has been cloned and sequenced. the derived amino acid sequence of 562 residues shows a high degree of homology to the previously published gr sequences from human erythrocytes and from two prokaryotes: escherichia coli and pseudomonas aeruginosa. the pea enzyme differs from other grs in having an n-terminal leader sequence of about 60-70 residues which may be a chloroplast transit peptide and a 20 amino acid c-terminal extension of unknown function. | 1992 | 1303792 |
| characterization of dna sequences that mediate nuclear protein binding to the regulatory region of the pisum sativum (pea) chlorophyl a/b binding protein gene ab80: identification of a repeated heptamer motif. | two protein factors binding to the regulatory region of the pea chlorophyl a/b binding protein gene ab80 have been identified. one of these factors is found only in green tissue but not in etiolated or root tissue. the second factor (denominated abf-2) binds to a dna sequence element that contains a direct heptamer repeat tctcaaa. it was found that presence of both of the repeats is essential for binding. abf-2 is present in both green and etiolated tissue and in roots and factors analogous to a ... | 1992 | 1303797 |
| effects of phosphodiesterase inhibitors caffeine and pentoxifylline on spontaneous and stimulus-induced acrosome reactions in human sperm. | to determine whether the phosphodiesterase inhibitors caffeine and pentoxifylline influence the acrosome reaction in the conditions in which they are currently used as sperm movement enhancers. | 1992 | 1333994 |
| influence of different substrates in detoxification activity of adult rat hepatocytes in long-term culture: implications for transplantation. | substrates used to immobilize hepatocytes for transplantation govern attachment and long-term metabolic activity of these cells. the choice of these substrates is based on the familiarity with proteinaceous materials that are constituents of the extracellular matrix. the use of substrates that recognize carbohydrates on the cell surface may provide an alternative method to attach adult mammalian hepatocytes. in this study, immobilized lectins on tissue culture plasticware were used to support he ... | 1992 | 1344292 |
| cloning and characterization of the p subunit of glycine decarboxylase from pea (pisum sativum). | a pea leaf cdna library constructed in lambda gt11 was screened with an antibody raised to the p subunit of glycine decarboxylase. one of the positive clones isolated was sequenced and shown to contain an open reading frame, which encoded the entire p subunit polypeptide. aligning the deduced amino acid sequence with the amino acid sequence determined directly from the nh2 terminus of the mature p subunit shows the presence of a putative 86 amino acid leader sequence, presumably required for imp ... | 1992 | 1347530 |
| identification, characterization, and dna sequence of a functional "double" groes-like chaperonin from chloroplasts of higher plants. | chloroplasts of higher plants contain a nuclear-encoded protein that is a functional homolog of the escherichia coli chaperonin 10 (cpn10; also known as groes). in pea (pisum sativum), chloroplast cpn10 was identified by its ability to (i) assist bacterial chaperonin 60 (cpn60; also known as groel) in the atp-dependent refolding of chemically denatured ribulose-1,5-bisphosphate carboxylase and (ii) form a stable complex with bacterial cpn60 in the presence of mg.atp. the subunit size of the pea ... | 1992 | 1356267 |
| xyloglucan oligosaccharide alpha-l-fucosidase activity from growing pea stems and germinating nasturtium seeds. | [14c]fucose-labelled xyloglucan (xg) was synthesized from tamarind seed xg by incubating it with gdp-[14c]fucose plus solubilized pea fucosyltransferase, and [14c]fucose-labelled xg nonasaccharide was prepared from the parent hemicellulose by partial hydrolysis with fungal cellulase. alpha-l-fucosidase activity was readily detected in crude enzyme extracts of growing regions of etiolated pea stems (pisum sativum) and in cotyledons of germinating nasturtium seedlings (tropaeolum majus) using the ... | 1991 | 1367786 |
| comparison of the n-linked oligosaccharide structures of the two major human myelin glycoproteins mag and p0: assessment of the structures bearing the epitope for hnk-1 and human monoclonal immunoglobulin m found in demyelinating neuropathy. | the epitope for hnk-1 and patient's monoclonal autoantibodies in demyelinating polyneuropathy associated with immunoglobulin m gammopathy is borne by different types of n-linked oligosaccharide structures in human p0 and myelin-associated glycoprotein (mag). fourteen glycopeptide fractions bearing different oligosaccharide structures were obtained from either mag or p0 glycopeptides by serial lectin affinity chromatography on concanavalin a-sepharose, phaseolus vulgaris erythrophytohemagglutinin ... | 1992 | 1371150 |
| interaction of lectins with human ige: ige-binding property and histamine-releasing activity of twelve plant lectins. | we examined the ige-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin a (con a), lens culinaris hemagglutinin (lch), pisum sativum agglutinin (psa), wheat germ agglutinin (wga), soybean agglutinin (sba), bauhinia purpurea agglutinin (bpa), peanut agglutinin (pna), ricinus communis agglutinin i (rca-i), lotus tetragonolobus agglutinin (lotus a), ulex europeus agglutinin i (uea-i), phytohemagglutinin e (pha-e) and phytohemagglutinin l (pha-l) ... | 1992 | 1378039 |
| the acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content. | in this study, we have evaluated the relationship between the acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. eighteen samples of follicular fluid were obtained during egg retrieval in six patients undergoing assisted fertilization. motile spermatozoa were incubated in modified tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 x 10(7) cells/ml. in a single experiment, aliquots of a semen specimen were simultaneously treated ... | 1992 | 1379265 |
| distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using pisum sativum agglutinin. | when western blots of human sperm proteins solubilized by acid extraction (presumably mainly acrosomal proteins) or by sodium dodecyl sulfate (sds) were probed with biotin-conjugated pisum sativum agglutinin (psa), distinct sets of proteins were labelled in both preparations. when smears of human spermatozoa were treated with methanol either for 30 s or for 15 min and then exposed to fitc-conjugated psa, the resulting fluorescence pattern essentially depended on the time of methanol treatment. w ... | 1992 | 1383539 |
| molecular dissection of structure and function in the lipopolysaccharide of rhizobium leguminosarum strain 3841 using monoclonal antibodies and genetic analysis. | following treatment with nitrosoguanidine, mutant derivatives of rhizobium leguminosarum strain 3841 were isolated which failed to react with afrc mac 203. this monoclonal antibody normally recognizes a strain-specific lipopolysaccharide epitope which is developmentally regulated during legume nodule differentiation. structural modification of lipopolysaccharide (lps) was analysed by examining reactivity with a range of monoclonal antibodies with different epitope specificities, and also by anal ... | 1992 | 1383672 |
| selective expression of a progesterone receptor on the human sperm surface. | to visualize progesterone (p) binding sites on the sperm surface, examine the relationship between hormone binding and hormone action (acrosome reaction), and determine the size of the hormone-responsive sperm subpopulation. | 1992 | 1385223 |
| true protein digestibility and amounts of endogenous protein measured with the 15n-dilution technique in piglets fed on peas (pisum sativum) and common beans (phaseolus vulgaris). | the faecal and ileal true protein digestibilities of the raw pea (pisum sativum) varieties finale and frijaune and the ileal true protein digestibility of steam-processed common beans (phaseolus vulgaris) were measured in piglets using the 15n-dilution technique. the faecal true protein digestibility of both pea varieties was about 97. the ileal true protein digestibility was between 93 and 95, indicating that the pea protein is almost completely enzymically digested in the small intestine. the ... | 1992 | 1390596 |
| [pea (pisum sativum) genes, participating in symbiosis with nitrogen-fixing bacteria. iii. study of the structure of the enod12 early nodulin gene for various types of peas using the polymerase chain reaction (pcr)]. | we have determined the length of early noduline gene enod12 in various varieties and lines of pea (pisum sativum) using the polymerase chain reaction (pcr). it was demonstrated that promoter regions of enod12a and enod12b genes in line 2150 (afghanistan) are longer than these in variety "feltham first". the disparity is 14 bp. when studying these genes in 7 different lines and varieties of pea it was found that enod12a gene is more variable in size than the enod12b gene. we showed the possibilit ... | 1992 | 1406614 |
| defective function of a nongenomic progesterone receptor as a sole sperm anomaly in infertile patients. | to compare the function of a novel nongenomic progesterone (p) receptor on the human sperm surface (mediating the p-induced acrosome reaction) in spermatozoa from fertile donors and from infertile patients. to examine the possible implication of defective p receptor function as an etiologic factor in unexplained male infertility. | 1992 | 1426326 |
| the fate of foreign dna associated with pig sperm following the in vitro fertilization of zona-free hamster ova and zona-intact pig ova. | the aim of this study was to determine the fate of foreign dna molecules bound to porcine sperm that had been capacitated and acrosome reacted in vitro using calcium ionophore and then used in the in vitro fertilization of zona-free hamster ova and zona-intact pig ova. fluoresceinated pisum sativum agglutinin (psa) labeling was used to differentiate between acrosome-intact and acrosome-reacted sperm. this revealed that up to 80% of the sperm treated with calcium ionophore were acrosome reacted. ... | 1992 | 1456841 |
| direct effects of progesterone and antiprogesterone on human sperm hyperactivated motility and acrosome reaction. | to study the direct effects of progesterone (p) and its antagonist ru486 (mifepristone) on sperm hyperactivation (ha) and acrosome reaction. | 1992 | 1459270 |
| expression of the pea metallothionein-like gene psmta in escherichia coli and arabidopsis thaliana and analysis of trace metal ion accumulation: implications for psmta function. | the psmta gene from pea (pisum sativum) shares similarity with metallothionein (mt) genes and related sequences have also been isolated from a number of other higher-plant species. the proteins encoded by these genes have not yet been purified from plants and their functions remain unclear although, by analogy to mt, roles in the metabolism and detoxification of metal ions have been proposed. by contrast, correlation between transcript abundance and fe availability has led to an alternative prop ... | 1992 | 1463837 |
| mutational analysis of pea lectin. substitution of asn125 for asp in the monosaccharide-binding site eliminates mannose/glucose-binding activity. | as part of a strategy to determine the precise role of pea (pisum sativum) lectin, psl, in nodulation of pea by rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using pcr. introduction of a specific mutation, n125d, into a central area of the sugar-binding site resulted in complete loss of binding of psl to dextran as well as of mannose/glucose-sensitive haemagglutination activity. as a control, substitution of an adjacen ... | 1992 | 1463840 |
| nucleosomal structure and histone h1 subfractional composition of pea (pisum sativum) root nodules, radicles and callus chromatin. | higher-order packaging of dna in chromatin structures could be an essential step in the complex chain of events leading to activation/repression of eukaryotic gene expression. with the goal to investigate this aspect of transcriptional regulation of plant genes involved in symbiotic interactions between legumes and rhizobia we analyze here the molecular parameters of chromatin structure in functioning root nodules, callus and radicles of pea. morphological intactness and the typical nucleosomal ... | 1992 | 1463843 |
| amino-acid sequence and predicted three-dimensional structure of pea seed (pisum sativum) ferritin. | the iron storage protein, ferritin, is widely distributed in the living kingdom. here the complete cdna and derived amino-acid sequence of pea seed ferritin are described, together with its predicted secondary structure, namely a four-helix-bundle fold similar to those of mammalian ferritins, with a fifth short helix at the c-terminus. an n-terminal extension of 71 residues contains a transit peptide (first 47 residues) responsible for plastid targetting as in other plant ferritins, and this is ... | 1992 | 1472006 |
| schizosaccharomyces pombe mitochondria: morphological, respiratory and protein import characteristics. | a technique is described for the isolation and purification of intact, respiratory-competent mitochondria from schizosaccharomyces pombe. the purified mitochondria are capable of oxidizing nadh and succinate as respiratory substrates, indicating the presence of succinate dehydrogenase and an nadh dehydrogenase located on the outer surface of the inner membrane. mitochondria display good respiratory control with an adp/o ratio of < 2. respiratory activity is linearly dependent upon the redox pois ... | 1992 | 1481570 |
| nadph: protochlorophyllide oxidoreductases in white pine (pinus strobus) and loblolly pine (p. taeda). evidence for light and developmental regulation of expression and conservation in gene organization and protein structure between angiosperms and gymnosperms. | nadph: protochlorophyllide oxidoreductase (pchlide reductase, ec 1.6.99.1) catalyzes the light-dependent reduction of protochlorophyllide in higher plants. cloned cdnas encoding two distinct pchlide reductases were isolated from a lambda gt11 library constructed from poly(a)+ rna prepared from the cotyledons of dark-grown white pine (pinus strobus) seedlings and a nuclear gene (lpcr) analogous to one of these cdnas has been characterized from loblolly pine (p. taeda). the pine gene encodes an ap ... | 1992 | 1494355 |
| development and characterization of monoclonal antibodies against a mucin-type glycoprotein. | the preparation of greater than 30 different hybridomas, all secreting igm class antibodies against epiglycanin, a glycoprotein at the surface of the mouse mammary carcinoma cell line ta3-ha, is described. the specificities of 10 of the antibodies, with affinity constants in the range of 10(8)-10(10) l/mol were compared in an enzyme competitive binding assay. the affinity of epiglycanin was strongly reduced for all antibodies tested by incubation with periodate (10 mm, 4 degrees c) and was reduc ... | 1992 | 1498419 |
| molecular cloning of phenylalanine ammonia-lyase cdna from pisum sativum. | | 1992 | 1515609 |
| a homolog of escherichia coli reca protein in plastids of higher plants. | studies of chloroplast dna variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. however, no studies have been done of the biochemical pathways involved. using a part of a cyanobacterial reca gene as a probe in southern blots, we have found homologous sequences in total dna from pisum sativum and arabidopsis thaliana and in a cdna library from arabidopsis. a cdna was cloned and sequenced, and its predicted am ... | 1992 | 1518831 |
| cloning of the gene (gdch) encoding h-protein, a component of the glycine decarboxylase complex of pea (pisum sativum l.). | h-protein is the lipoyl-protein component of the glycine decarboxylase complex, which catalyses, with serine hydroxymethyltransferase, the mitochondrial step of photorespiration in plants. we have isolated and characterized the gene (gdch) encoding the h-protein in pea (pisum sativum l.). the h-protein gene is distributed in a stretch of about 1.55 kbp and contains three introns (75, 64 and 185 bp) located in the coding region. no intervening sequences were detected in the 5' and 3' non-coding r ... | 1992 | 1530594 |
| in vitro reconstitution of a light-harvesting gene product: deletion mutagenesis and analyses of pigment binding. | ab96, a gene encoding a pisum sativum chlorophyll a/b binding protein [coruzzi et al. (1983) j. biol. chem. 258, 1399-1402], can be expressed in escherichia coli and reconstituted with pigments by the procedure described by plumley and schmidt [(1987) proc. natl. acad. sci. u.s.a. 84, 146-150]. following purification by polyacrylamide gel electrophoresis, the reconstituted pigment-protein complex (cp2) is shown to have similar pigment-binding characteristics to native cp2 complexes isolated from ... | 1992 | 1547218 |
| the major kurloff cell glycoproteins: lectin affinities, glycosidase susceptibilities and relationship with the sialylated acid phosphatases of the kurloff body. | urea-soluble fractions from purified kurloff cells (kc) were analysed by affinoblotting. lectin reactivities were quasi-exclusively confined to the 30-35 kda major glycoproteins (mgps) (responsible for the pas positivity of the kurloff body) with strong affinities for canavalia ensiformis lectin, phaseolus vulgaris erythroagglutinin and sambucus nigra (sna), pisum sativum, triticum vulgaris and ulex europeus agglutinins. these data were consistent with the presence, among the kc mgps, of large a ... | 1992 | 1581339 |
| identification and localization of multiple forms of serine hydroxymethyltransferase in pea (pisum sativum) and characterization of a cdna encoding a mitochondrial isoform. | serine hydroxymethyltransferase (shmt) has been purified from the mitochondria of green pea leaves. activity can be fractionated into two distinct peaks by ion exchange chromatography. while these two forms of the enzyme are immunologically indistinguishable, immunoinhibition experiments show the presence of a distinct non-mitochondrial third form of the enzyme to also be present in green pea leaves. while this mitochondrial form of shmt is abundant in leaves it is absent from roots, although th ... | 1992 | 1618853 |
| digestion of complex carbohydrates and large bowel fermentation in rats fed on raw and cooked peas (pisum sativum). | rats were fed on four semi-purified diets in which raw or cooked peas (pisum sativum var. sentinel) provided 250 or 500 g/kg diet. pressure-cooking of peas followed by rapid freezing and freeze-drying increased the proportion of starch resistant to alpha-amylase (ec 3.2.1.1) without previous treatment with dimethyl sulphoxide. there were only minor effects of cooking on the digestibilities of non-starch polysaccharides and their constituent sugars. with the higher dietary pea concentration there ... | 1992 | 1622985 |
| histochemical analysis of rat testicular glycoconjugates. 1. subsets of n-linked saccharides in seminiferous tubules. | the distribution of n-linked glycans in rat testis has been probed using a panel of lectins derived from galanthus nivalis (snowdrop, gna), canavalia ensiformis (jack bean, con a), lens culinaris (lentil, lca), pisum sativum (garden pea, psa) and phaseolus vulgaris, erythro- and leucoagglutinins (kidney bean, epha and lpha). several classes of n-linked glycan were identified in the spermatogenic series, and during differentiation into spermatozoa they altered in both their pattern of distributio ... | 1992 | 1634371 |
| light represses transcription of asparagine synthetase genes in photosynthetic and nonphotosynthetic organs of plants. | asparagine synthetase (as) mrna in pisum sativum accumulates preferentially in plants grown in the dark. nuclear run-on experiments demonstrate that expression of both the as1 and as2 genes is negatively regulated by light at the level of transcription. a decrease in the transcriptional rate of the as1 gene can be detected as early as 20 min after exposure to light. time course experiments reveal that the levels of as mrna fluctuate dramatically during a "normal" light/dark cycle. this is due to ... | 1991 | 1681424 |
| molecular cloning, transcriptional characterization, and sequencing of cdna encoding the h-protein of the mitochondrial glycine decarboxylase complex in peas. | the glycine decarboxylase multienzyme complex is located in the mitochondrial matrix and catalyzes a key reaction of the photorespiratory c-2 cycle of c3 plants. the cdna encoding the smallest subunit, the 13,900-dalton h-protein, of the glycine decarboxylase complex from pea (pisum sativum) leaves was cloned, identified, characterized, and sequenced. the 678-nucleotide sequence contained a 495-nucleotide open reading frame capable of encoding the 165-amino-acid h-protein precursor. the n termin ... | 1990 | 1688555 |
| assessment of the viability and acrosome status of fresh and frozen-thawed human spermatozoa using single-wavelength fluorescence microscopy. | the purpose of this study was to use fluorescence microscopy to determine the viability and acrosome status of fresh and frozen-thawed human spermatozoa. sperm cells were stained with the viability stains hoechst 33258 (h33258) alone, or propidium iodide (pi) alone, and pi in combination with fitc-conjugated pisum sativum agglutinin (psa). the psa stains the acrosome contents of permeabilized acrosome-intact sperm. viability by fluorescence microscopy was compared to conventional eosin nigrosin ... | 1990 | 1701095 |
| suppression of nodulation gene expression in bacteroids of rhizobium leguminosarum biovar viciae. | the expression of nod genes of rhizobium leguminosarum bv. viciae in nodules of pisum sativum was investigated at both the translational and transcriptional levels. by using immunoblots, it was found that the levels of noda, nodi, node, and nodo proteins were reduced at least 14-fold in bacteriods compared with cultured cells, whereas nodd protein was reduced only 3-fold. northern (rna) blot hybridization, rnase protection assays, and in situ rna hybridization together showed that, except for th ... | 1991 | 1712355 |
| an altered constitutive peptide in sym 5 mutants of pisum sativum l. | mutational analysis of pisum sativum l. was used to search for constitutive proteins that might function in nodule formation. the sym 5 locus is a mutational hot spot, represented by seven independently derived mutant lines with decreased nodulation. comparison of two-dimensional polyacrylamide gels of in vitro-translated root rna showed a consistent difference in the migrational pattern of one peptide. in the nodulating parental cultivar 'sparkle', a 66 kda peptide had a pi of 5.9. in four of t ... | 1990 | 1714314 |
| glycoprotein changes in the rat sperm plasma membrane during maturation in the epididymis. | to investigate surface glycoprotein changes during post-testicular maturation, plasma membranes were isolated from proximal caput, distal caput, and cauda epididymal rat spermatozoa. membrane glycoproteins were identified on western blots of sds-page fractionated samples using biotinylated lectins and vecta-stain reagents; these were compared to glycoproteins present in cauda epididymal luminal fluid. lens culinaris agglutinin, pisum sativum agglutinin, peanut agglutinin, wheat germ agglutinin, ... | 1991 | 1716114 |
| lectin binding in skeletal muscle. evaluation of alkaline phosphatase conjugated avidin staining procedures. | cryostat sections from rat gracilis muscles were incubated with different biotinylated lectins: con a (concanavilin a), wga (wheat germ agglutinin), sba (soybean agglutinin), gs i and gs ii (griffonia simplicifolia agglutinin), lca (lens culinaris agglutinin), pna (peanut agglutinin) and psa (pisum sativum agglutinin). the sections were subsequently treated with alkaline phosphatase conjugated avidin. the lectin binding sites were visualized after incubation in substrate media containing: (1) 5- ... | 1991 | 1717410 |
| artificial induction of exocytosis in bull sperm. | we have investigated an exocytotic event, the acrosome reaction (ar), induced by treatment of bovine sperm with vesicles composed of dilauroyl phosphatidylcholine (pc12). cell membrane permeability barriers (dye exclusion), acrosomal status (pisum sativum (psa) lectin binding), and intracellular ca2+ (fluo3 fluorescence) were evaluated utilizing flow cytometry and fluorescence microscopy. by these methods the ar is resolved into four kinetically distinct steps: (a) pc12 transfer to the sperm pla ... | 1992 | 1727647 |
| identification of lectin binding proteins in human tears. | the identity of glycoproteins in stimulated normal human tears was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of tears onto minigels, blotting, and subsequent incubation with different biotinylated lectins (concanavalin a [con a], peanut agglutinin [pna], glycine max agglutinin [sba], phaseolus vulgaris agglutinin, wheat germ agglutinin [wga, native form], artocarpus integrifolia agglutinin [jacalin], and pisum sativum agglutinin). control proteins inclu ... | 1991 | 1748557 |
| molecular genetics of pseudomonas syringae pathovar pisi: plasmid involvement in cultivar-specific incompatibility. | a mutant (pf24) of the race 1 strain, 299a, of pseudomonas syringae pv. pisi has been characterized in terms of its interactions with pea (pisum sativum) cultivars. the mutant showed a changed reaction (avirulence to virulence) with a group of pea cultivars, including cvs. belinda and puget, previously thought to contain resistance genes r1 and r3. avirulence towards cv. puget was restored by transfer of any one of five cosmid clones from a race 3 (strain 870a) gene library to a rifampicin-resis ... | 1991 | 1748876 |
| a plant metallothionein produced in e. coli. | a metallothionein cdna was generated from pea (pisum sativum l.) roots, amplified by pcr and inserted into a plasmid for expression in e. coli. purification of the resultant product generated 3 pools of cadmium-containing material after deae-cellulose chromatography. the amino acid composition of each was in excellent agreement with that predicted for pea metallothionein. a cadmium content of approximately 6 g.atoms per mole of protein was estimated. n-terminal sequence analysis revealed that th ... | 1991 | 1765150 |
| natural killer (nk) cell activating factor produced by a human t-cell hybridoma. | a human t-cell hybridoma (kc8-1.10), whose culture supernatant augments peripheral blood lymphocytes (pbl)-mediated spontaneous cytotoxicity against k562 cells, was established. this activity [natural killer (nk) cell activating activity] appears to be not due to interferon-gamma (ifn-gamma) and interleukin-2 (il-2) for the following reasons: 1) kc8-1.10 produced negligible or small amounts of ifns and il-2. 2) the nk cell activating activity in the kc8-1.10 culture supernatant was not neutraliz ... | 1991 | 1775099 |
| efficacy of seed-based media for the mould-yeast conversion of blastomyces dermatitidis. | the efficacy of 20 seed-based media is reported for the in vitro mould-yeast conversion of blastomyces dermatitidis, employing pharmamedia agar, peptone glucose agar, glucose agar and water agar as controls. the mould-yeast conversion varied significantly according to the culture medium, fungal strains and incubation period (p less than 0.01). garden-pea, chick-pea, cow-pea, soybean, peanut, green gram, french bean, lentil, okra and cottonseed converted all of the 7 b. dermatitidis test strains ... | 1991 | 1780002 |
| an escherichia coli protein with homology to the h-protein of the glycine cleavage enzyme complex from pea and chicken liver. | the nucleotide sequence of an escherichia coli gene which presumably encodes the h-protein of the glycine cleavage (gcv) enzyme complex is presented. the gene, designated gcvh, encodes a polypeptide of 128 amino acids with a calculated molecular weight of 13,665 daltons. the translation start site was determined by n-terminal amino acid sequence analysis of a gcvh-lacz encoded fusion protein. the e. coli h-protein shows extensive homology with the h-proteins from the pea (pisum sativum) and the ... | 1991 | 1802033 |
| [pea (pisum sativum) genes participating in symbiosis with nitrogen-fixing bacteria. ii. nucleotide sequence of cdna and parts of the late nodulin-6 (psnod6) gene]. | certain characteristics of late noduline gene from pea-nod6 were investigated as part of works of characterization of higher plant genes taking part in symbiotic nitrogen fixation. the complete 450 b.p. long cdna was sequenced, it's coding sequence includes the open reading frame. the part of dna containing the corresponding gene from the genomic clone was also sequenced. the predicted nod6 amino acid sequence has been analyzed and do not reveal the significant homology with any known protein. | 1991 | 1813810 |
| [synthesis of sorbent from cross-linked starch for affinity purification of glucose(mannose)-specific lectins]. | cross-linked starch gel for the affinity chromatography of d-glucose (d-mannose)-specific lectins is suggested. in order to optimize hydrodynamic properties of gel 30% starch has been hydrolysed by hci at 70 degrees c during 60 min and then cross-linked by epichlorohydrin under alkaline conditions. every 100 g of starch require 18 ml of epichlorohydrin and 36 ml of 8 n koh. the gel obtained has been successfully used for the purification of lectins from pisum sativum l., lens culinaris l., vicia ... | 1991 | 1816693 |
| induced plant responses to pathogen attack. analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (glycine max l. merr. cv. harosoy 63). | in soybean (glycine max l.), pathogen attack induces the formation of glyceollin-type phytoalexins. the biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. screening of a soybean cdna library from elicitor-induced rna in lambda gt11 yielded two classes of reductase-specific clones. the deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. four clones of class a were ... | 1991 | 1840523 |
| purification of beta-glucuronidase and structural assessment of the carbohydrate chains by lectin affinity immunoelectrophoresis. | the purification of rat liver beta-glucuronidase from a lysosomal fraction by methods including affinity chromatography, chromatofocusing and preparative page steps is described. molecular weights of 300,000 and 150,000 were estimated by two dimensional gradient page/immunoelectrophoresis of the lysosomal extract. isoelectrofocusing in agarose gel followed by immunoelectrophoresis in the second dimension revealed the presence of at least five maxima in the range ph 4.3-7.4. the structural assess ... | 1991 | 1841676 |
| comparative response of pisum sativum nodulated with indigenous soil rhizobium populations and/or co-inoculated with a rhizobium leguminosarum strain. i. acetylene-reducing, dihydrogen- and carbon dioxide-evolving activities. | no significant differences in the acetylene-reducing activity and evolution of h2 and co2 from nodulated roots of pisum sativum inoculated with soil rhizobium populations from two soils with different acidities (ruzynĕ soil 7.6; lukavec soil 4.9) were observed. rhizobium population from lukavec soil formed nodules, exhibiting a higher h2 evolution. co-inoculation with the hup+ strain 128c30 (7 x 10(7) cells per seedling) eliminated, to some extent, the effect of soil populations on physiological ... | 1991 | 1841862 |
| pea lectin is correctly processed, stable and active in leaves of transgenic potato plants. | a gene encoding the preproprotein of the pea (pisum sativum) lectin was expressed in transgenic potato plants using a cauliflower mosaic virus (camv) 35s promoter or a tobacco ribulose bisphosphate carboxylase small subunit (ssrubisco) promoter. presence of the pea lectin to levels greater than 1% of total soluble leaf protein was detected by radioimmunoassay (ria). the pattern of expression derived from the two promoters was established using both ria and a squash-blot immunolocalisation techni ... | 1991 | 1868225 |
| effect of sperm-antibodies on acrosome reaction of human sperm used for the hamster egg penetration assay. | the effect of anti-sperm antibodies (asa) on the rate of acrosome reactions (ar) during "in vitro" capacitation of human sperm used for the hamster egg penetration assay (hepa) was assessed. motile sperm suspensions from donors were exposed to several sera and seminal plasma with sperm head-directed asa, then they were washed and capacitated "in vitro." after capacitation, the proportion of acrosome-reacted viable sperm was assessed by staining with fluoresceinated pisum sativum agglutinin and s ... | 1991 | 1872955 |
| monitoring by epifluorescence microscopy of organelle dna fate during pollen development in five angiosperm species. | the fates of mitochondrial and plastid nucleoids during pollen development in six angiosperm species (antirrhinum majus, glycine max, medicago sativa, nicotiana tabacum, pisum sativum, and trifolium pratense) were examined using epifluorescence microscopy after double staining with 4',6-diamidino-2- phenylindole (dapi) to stain dna and with a potentiometric dye (either dioc7 or rhodamine 123) for visualization of metabolically active mitochondria. from the pollen mother cell stage to the microsp ... | 1991 | 1879613 |
| cloning of the pea cdc2 homologue by efficient immunological screening of pcr products. | a homologue of the ubiquitous eukaryotic cell cycle regulatory gene, cdc2, has been cloned from pisum sativum, the garden pea. a novel immunological strategy was devised and implemented for screening pcr products generated by degenerate oligonucleotide primers. we used pcr to construct a deletion derivative of an escherichia coli expression plasmid carrying the schizosaccharomyces pombe cdc2 gene. the deleted segment encoded the domain recognized by monoclonal antibody mab-j4, a reagent which al ... | 1991 | 1883993 |
| solubilization and properties of gdp-fucose: xyloglucan 1,2-alpha-l-fucosyltransferase from pea epicotyl membranes. | gdp-fucose:xyloglucan 1,2-alpha-l-fucosyltransferase from pea (pisum sativum) epicotyl microsomal membranes was readily solubilized by extraction with the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (chaps). when using gdp-[14c]fucose as fucosyl donor and tamarind xyloglucan (xg) as acceptor, maximum activation was observed at 0.3% (w/v) chaps and the highest yield of solubilized activity at 0.4%. the reaction product was hydrolyzed by trichoderma cellulase t ... | 1991 | 1898102 |
| differences in the lectin-binding patterns of the periportal and perivenous endothelial domains in the liver sinusoids. | we have studied the distribution patterns of carbohydrate terminals on the endothelial surface of the mouse liver microvasculature. for this purpose, a wide battery of fitc lectins specific to glucose, mannose, galactose, fucose, n-acetyl-neuraminic acid, n-acetyl-galactosamine and n-acetyl-glucosamine residues were incubated on liver cryostat sections or intraportally perfused under physiological conditions. all the resulting hepatic sections were examined under fluorescent microscopy and confo ... | 1991 | 1906044 |
| use of sperm viability and acrosomal status assays in combination with immunofluorescence technique to ascertain surface expression of sperm antigens. | sperm antigens are being sought for the development of prototype contraceptive vaccines. for vaccine-induced antisperm antibodies to inhibit the function of viable sperm, target antigens must be expressed on the sperm surface before, during or after the acrosome reaction. the purpose of this study was to develop an approach to detect accurately surface binding of antibodies to viable acrosome-intact or acrosome-reacted sperm, and to confirm sperm surface binding characteristics of a panel of ant ... | 1991 | 1908520 |
| further studies of lectin binding by villous and extravillous trophoblast in normal and pathological pregnancy. | examination of human pregnancy tissues with a panel of lectins provides the opportunity to probe different aspects of carbohydrate structure. nine biotinylated lectins [concanavalin a (con a), wheat germ agglutin (wga), lens culinaris a (lch-a), pisum sativum (psa), phaseolus vulgaris (pha-e and pha-l), ulex europaeus 1 (uea1), griffonia simplicifolia (gsi and gsii)] were used to investigate the lectin binding of human trophoblast in normal, tubal, and molar pregnancy. all lectins except uea1 bo ... | 1991 | 1917274 |
| glycoconjugates of the lateral prostate of the guinea pig: a lectin histochemical study. | the present study examined the glycoconjugates of the lateral prostate using a battery of lectins. the results indicated that the secretory epithelium was rich in mannose (man), n-acetylglucosamine (glcnac), galactose (gal), n-acetylgalactosamine (galnac), and complex oligosaccharides. con a (concanavalin a), lca (lens culinaris agglutinin), psa (pisum sativum agglutinin), wga (wheat germ agglutinin), pwm (pokeweed mitogen), rca-i (racinus communis isolectin i), and pha-p (phaseolus vulgaris agg ... | 1991 | 1923963 |
| induction of 6a-hydroxymaackiain 3-o-methyltransferase and phenylalanine ammonia-lyase mrna translational activities during the biosynthesis of pisatin. | the isoflavonoid phytoalexin pisatin is synthesized by pea (pisum sativum l.) in response to microbial infection and certain other forms of stress. the terminal step in the biosynthesis of pisatin is catalyzation by the (+)-6a-hydroxymaackiain 3-o-methyltransferase (hmkmt). this enzyme, identified as a protein of mr 43,000 by photoaffinity labeling (preisig et al. (1989) plant physiol. 91, 559-566), was purified 280-fold from cucl2-stressed pea seedlings and subjected to preparative sodium dodec ... | 1991 | 1929414 |
| intrinsic labelling of different foods with stable isotope of zinc (67zn) for use in bioavailability studies. | intrinsically-labelled foods are required to validate extrinsic-labelling techniques used to study the bioavailability of trace elements. wheat (triticum aestivum), peas (pisum sativum), goat's milk, human milk, eggs and chicken meat were selected for intrinsic-labelling studies with 67zn. peas were grown hydroponically in enriched nutrient solution and wheat was grown in sand and watered with enriched nutrient solution. some of the wheat plants were also given stem injections of 67zn solution. ... | 1991 | 1931906 |
| apparent zinc absorption by rats from foods labelled intrinsically and extrinsically with 67zn. | a variety of foods (peas (pisum sativum), chicken meat, eggs, goat's milk, human milk) enriched with the stable isotope 67zn were prepared by means of intrinsic- and extrinsic-labelling procedures. they were fed to rats and apparent absorption of 67zn determined from faecal excretion measurements using thermal ionization mass spectrometry. there were significant differences in the absorption of the extrinsic and intrinsic label which differed in magnitude between the foods tested. the extrinsic ... | 1991 | 1931907 |
| expression of the pea gene psmta in e. coli. metal-binding properties of the expressed protein. | the pea (pisum sativum l.) gene psmta has an orf encoding a predicted protein with sequence similarity to class i metallothioneins (mts). to examine the metal-binding properties of the psmta protein it has been expressed in e. coli as a carboxyterminal extension of glutathione-s-transferase (gst). metal ions were associated with the expressed protein when purified from lysates of e. coli grown in metal supplemented media. the ph of half-dissociation of zn, cd and cu ions from the recombinant fus ... | 1991 | 1959626 |
| dark-induced and organ-specific expression of two asparagine synthetase genes in pisum sativum. | nucleotide sequence analysis of cdnas for asparagine synthetase (as) of pisum sativum has uncovered two distinct as mrnas (as1 and as2) encoding polypeptides that are highly homologous to the human as enzyme. the amino-terminal residues of both as1 and as2 polypeptides are identical to the glutamine-binding domain of the human as enzyme, indicating that the full-length as1 and as2 cdnas encode glutamine-dependent as enzymes. analysis of nuclear dna shows that as1 and as2 are each encoded by sing ... | 1990 | 1968003 |