| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| on the receptors of human red cells reacting with phaseolus coccineus l. lectin. | in order to elucidate receptors of proteolytic enzyme-treated red cells which react with phaseolus coccineus l. lectin, the receptors prepared by affinity chromatography were serologically investigated. p. coccineus lectin had high agglutinin activity for bromelin-, papain- and pronase-treated red cells but that for the cells treated with ficin and trypsin was relatively low. analyses of chemical composition revealed that sialic acid of the receptors from normal red cells was considerably much a ... | 1979 | 494265 |
| the lectin binding sites on the membranes of the nuclear envelope, mitochondria and the cell surface of human lymphoblastoid cells. | the membranes of the cell surface, the endoplasmic reticulum, outer and inner mitochondrial leaflet and nuclear envelope were isolated from three human lymphoblastoid cell lines. membrane components were separated by dodecyl sulfate polyacrylamide gel electrophoresis and the gels incubated with the radioiodinated lectins from lentil, castor bean, scarlet runner bean, gorse seed and roman snail. after gel slicing and counting, the molecular weights of the lectin binding sites were determined. abo ... | 1979 | 527941 |
| immunosuppressive activity of phaseolus coccineus and phaseolus vulgaris extracts in mice. | lectin mixtures from 10 samples of phaseolus coccineus and phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (srbc) antibody response of the mouse. some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin a and phytohemagglutinin tested before in the same immune system. high dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the t ... | 1976 | 1086791 |
| [comparative study of the chemical composition and nutritive value of runner bean (phaseolus coccineus) and of common bean (phaseolus vulgaris)]. | scarlet beans (phaseolus coccineus) is an important food grain legume in the diet of rural populations living in the highlands of méxico, guatemala and other countries of latin america. the present study was conducted to obtain more chemical and nutritive data on this grain legume, because of its importance in agricultural production systems and due to the role it plays in rural diets. ten samples were purchased in the highland markets of guatemala, to compare their physical, chemical and nutrit ... | 1992 | 1308649 |
| immunocytochemical localization of the storage protein phaseolin in the embryo-suspensor of phaseolus coccineus. | antibodies against the bean storage protein, phaseolin, were induced in rabbits. sections of bean embryos of various age were incubated with the antibodies, and exposed to fluorescein isocyanate (fitc) conjugated goat anti-rabbit immunoglobulin. distinct positive signals were found in vacuoles of the suspensor giant cells. immunofluorescent staining of protein bodies in cotyledon cells, histochemical staining reactions of the sections, as well as gel electrophoretic analysis of the proteins, iso ... | 1991 | 1768728 |
| the characteristics of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferases from sansevieria trifasciata leaves and phaseolus coccineus stems. | three different molecular forms of pyrophosphate-dependent phosphofructokinase have been isolated: one from sansevieria trifasciata leaves and two from phaseolus coccineus stems. the form isolated from s. trifasciata has the molecular weight of about 115,000. the apparent molecular weights for the two forms from mung bean were approximately 220,000 and 450,000. all three forms have the same ph optima, an absolute requirement for mg2+ ions both in the forward and reverse reaction, but differ in t ... | 1987 | 2825454 |
| acute bovine pulmonary emphysema caused by the fungus fusarium semitectum. | acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse. | 1988 | 3388752 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk. | eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ... | 1983 | 6615758 |
| effect of lectins on mouse peritoneal macrophage phagocytic activity. | we studied the in vitro ability of lectin-treated murine peritoneal macrophages to attach and phagocytize particulate antigens. glucose and mannose specific lectins such as con-a and lentil lectin, as well as complex lactosamine residues specific lectins, such as phaseolus vulgaris var. cacahuate and phaseolus coccineus var. alubia, increased the macrophage phagocytic activity towards heterologous erythrocytes, whereas peanut agglutinin, a galactose-specific lectin, diminished the macrophage pha ... | 1994 | 7851961 |
| glycosylation pattern and enzyme activities in atrophic, angulated skeletal muscle fibres from ageing rats. | in this study enzyme activities and lectin binding patterns in skeletal muscle from very old rats were investigated in order to evaluate changes in enzyme activity or carbohydrate expression in senile muscle. activities for adenosine triphosphatase (atpase), succinic dehydrogenase, non-specific esterase and the binding pattern for 31 lectins were investigated in the soleus muscles from very old (36 months) and young (3 months) rats. in ageing muscles atrophic, angulated muscle fibres are frequen ... | 1994 | 8186892 |
| purification and partial characterization of a new anti-a1 lectin of phaseolus coccineus collected in oaxaca, mexico. | it is well known that phaseolus species contains lectins of various specificities 1-4. runner bean5 and different varieties of ph.coccineus described by ochoa et al6, are not specific to human blood groups. they react indistinctly with erythrocytes of the abo system. however the lectin of ph. lunatus (lima bean)7 is specific to the blood group type a, a classic example of that specificity. sometime ago, we discovered a new lectin in a variety of edible bean of ph. coccineus in the flora of the s ... | 1996 | 8744420 |
| protective mechanism of the mexican bean weevil against high levels of alpha-amylase inhibitor in the common bean. | alpha-amylase inhibitor (alpha ai) protects seeds of the common bean (phaseolus vulgaris) against predation by certain species of bruchids such as the cowpea weevil (callosobruchus maculatus) and the azuki bean weevil (callosobruchus chinensis), but not against predation by the bean weevil (acanthoscelides obtectus) or the mexican bean weevil (zabrotes subfasciatus), insects that are common in the americas. we characterized the interaction of alpha ai-1 present in seeds of the common bean, of a ... | 1996 | 8787024 |
| molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation. | a major catabolic pathway for the gibberellins (gas) is initiated by 2beta-hydroxylation, a reaction catalyzed by 2-oxoglutarate-dependent dioxygenases. to isolate a ga 2beta-hydroxylase cdna clone we used functional screening of a cdna library from developing cotyledons of runner bean (phaseolus coccineus l.) with a highly sensitive tritium-release assay for enzyme activity. the encoded protein, obtained by heterologous expression in escherichia coli, converted ga9 to ga51 (2beta-hydroxyga9) an ... | 1999 | 10200325 |
| regional localization of suspensor mrnas during early embryo development. | we investigated gene activity within the giant embryos of the scarlet runner bean (phaseolus coccineus) to gain understanding of the processes by which the apical and basal cells become specified to follow different developmental pathways after division of the zygote. we identified two mrnas, designated g564 and c541, that accumulate specifically within the suspensor of globular-stage embryos. g564 mrna accumulates uniformly throughout the suspensor, whereas c541 mrna accumulates to a higher lev ... | 2001 | 11701878 |
| [physical, culinary and nutritional characterization in dry bean from the highlands of mexico]. | in the improvement of a given crop species, knowledge on the grain quality and related traits in the progenitors used in the breeding process is needed to establish their usefulness as a source of a given character, and as well as to plan the proper combinations between progenitors. the aim of the present research was to characterize a group of 49 genotypes of phaseolus vulgaris and one of phaseolus coccineus, in relation to physical, cooking and nutritional grain traits. cultivar blanco tlaxcal ... | 2002 | 12184152 |
| the isolation of carbohydrate-binding peptide from scarlet runner bean lectin. | the carbohydrate-binding peptide fragment of scarlet runner bean (phaseolus coccineus var. rubronanus) lectin has been prepared by trypsin digestion. the carbohydrate-binding peptide was isolated from digested solution by affinity chromatography on thyroglobulin-sepharose column, bio-gel p-4 gel filtration column and reverse phase hplc on c-8 column. the fraction of peak i from hplc which bound specifically with man(8)glcnac(2) was demonstrated by using dot blot technique with [(3)h]- man(8)glcn ... | 1997 | 12219212 |
| sequence analysis of the oligosaccharides from scarlet runner bean lectin. | two oligosaccharide chains from scarlet runner bean (phaseolus coccineus var. rubronanus) lectin (pcl) were obtained by hydrazinolysis, bio-gel p-2 column chromatography, nabh(4) reduction and then separated on a bio-gel p-2 column. the oligosaccharides were methanolysed, acetylysed and then transformed into derivatives of trimethylsilane. gas chromatographic studies of these two derivatives showed that their compositions were: man(8)xylglcnac(2) (oligosaccharide i) and man(6)xylglcnac(2)fuc (ol ... | 1996 | 12232596 |
| activation tagging of a dominant gibberellin catabolism gene (ga 2-oxidase) from poplar that regulates tree stature. | we identified a dwarf transgenic hybrid poplar (populus tremula x populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. the cause of the phenotype was a hyperactivated gene encoding ga 2-oxidase (ga2ox), the major gibberellin (ga) catabolic enzyme in plants. the mutation resulted from insertion of a strong transcriptional enhancer near the transcription start site. overexpression of the poplar ga2ox gene (ptaga2 ... | 2003 | 12857810 |
| coccinin, an antifungal peptide with antiproliferative and hiv-1 reverse transcriptase inhibitory activities from large scarlet runner beans. | an antifungal peptide, designated coccinin, with a molecular mass of 7kda and an n-terminal sequence resembling those of defensins, was purified from the seeds of large scarlet runner beans (phaseolus coccineus cv. 'major'). the peptide isolated was unadsorbed on deae-cellulose, and adsorbed on affi-gel blue gel and mono s. the peptide excerted antifungal activity on a number of fungal species including botrytis cinerea, coprinus comatus, fusarium oxysporum, mycosphaerella arachidicola, physalos ... | 2004 | 15572193 |
| phaseococcin, an antifungal protein with antiproliferative and anti-hiv-1 reverse transcriptase activities from small scarlet runner beans. | from the seeds of small scarlet runner beans (phaseolus coccineus 'minor'), an antifungal protein with an n-terminal sequence homologous to those of defensins was isolated. the antifungal protein bound to affi-gel blue gel and mono s but it did not bind to deae-cellulose. it was further purified by gel filtration on a superdex peptide column. it exhibited a molecular mass of 5422 da as determined by mass spectrometry. the protein, designated as phaseococcin, suppressed mycelial growth in a numbe ... | 2005 | 15864329 |
| expression of two genes encoding gibberellin 2- and 3-oxidases in developing seeds of phaseolus coccineus. | we have isolated pcga3ox1, a cdna clone from developing runner bean (phaseolus coccineus) seeds that shows significant amino acid homology with the gibberellin (ga) 3-oxidases. a recombinant fusion protein of pcga3ox1 converted ga20 and ga9 to ga1 and ga4, respectively. in situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-stage embryos. pcga3ox1 mrna begins to accumulate in the epidermal cells of the embryo proper and is also ... | 2005 | 15894806 |
| a provisional database for the silicon content of foods in the united kingdom. | si may play an important role in bone formation and connective tissue metabolism. although biological interest in this element has recently increased, limited literature exists on the si content of foods. to further our knowledge and understanding of the relationship between dietary si and human health, a reliable food composition database, relevant for the uk population, is required. a total of 207 foods and beverages, commonly consumed in the uk, were analysed for si content. composite samples ... | 2005 | 16277785 |
| sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein banding patterns among rhizobium leguminosarum biovar phaseoli strains isolated from the mexican bean phaseolus coccineus. | several rhizobial strains were isolated from phaseolus coccineus root nodules and were determined to be rhizobium leguminosarum biovar phaseoli strains after reinfection of the same host plant. these strains were characterized by cultural procedures (growth on different carbon sources and intrinsic antibiotic resistance) and electrophoretic procedures (sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total proteins). our results showed that these rhizobia are very similar to each oth ... | 1993 | 16349098 |
| genetic structure of rhizobium etli biovar phaseoli associated with wild and cultivated bean plants (phaseolus vulgaris and phaseolus coccineus) in morelos, mexico. | the genetic structure of rhizobium etli biovar phaseoli was determined for five populations in three different locations in the state of morelos, mexico, by using starch gel electrophoresis for five to nine polymorphic loci. two populations were sampled during two different years from nodules of cultivated and wild common bean plants (phaseolus vulgaris). the three other populations were associated with wild runner beans (p. coccineus) and sampled during 1988. the rhizobium populations differ ge ... | 1994 | 16349234 |
| metabolism of h-gibberellin a(1) and h-gibberellin a(4) by phaseolus coccineus seedlings. | [(3)h]-gibberellin a(1) (ga(1)) and (3)h-ga(4) were applied separately to phaseolus coccineus seedlings grown under red light. (3)h-ga(1) was converted to a compound with gas-liquid radiochromatography retention times identical to those of ga(8). (3)h-ga(4) underwent conversion to at least three metabolites, none of which corresponded to ga(1-38). the rate of metabolism of (3)h-ga(4) was significantly higher than that of (3)h-ga(1). | 1975 | 16659025 |
| differences between adenosine triphosphatases from monocotylous and dicotylous plants. | the mitochondrial membrane-bound atpases of several plants were investigated. two distinct types were encountered. the mitochondrial atpases of castor bean (ricinus communis var. zanzibarensis, l.), cauliflower (brassica oleracea, l.), and scarlet runner (phaseolus coccineus, l.) were found to be inhibited by oligomycin, to have elevated molecular weights when separated from the organelles by ultrasonication and ammonium sulfate treatment and, subsequent to purification, to be cold-labile. on th ... | 1977 | 16659806 |
| distinction between nuclear satellite dnas and chloroplast dna in higher plants. | triton x-100 solubilized chloroplast dna but not nuclear dna from a mixture of chloroplasts and nuclei. the buoyant density of chloroplast dna was different from that of the satellite dna in all of the species examined (phaseolus coccineus, cucumis sativus, cucumis melo, antirrhinum majus, vicia faba, oenothera fruiticosa youngii). chloroplast dna constituted between 4.3% and 0.25% of the total leaf dna in these species, and was present as 5 to 20 copies in each chloroplast. | 1978 | 16660649 |
| gibberellins in suspensors of phaseolus coccineus l. seeds. | analysis of extracts from 6300 (1.2 grams fresh weight) phaseolus coccineus suspensors by combined gas chromatography-mass spectrometry has demonstrated the presence of five c(19)-gibberellins, ga(1), ga(4), ga(5), ga(6), ga(8), and one c(20)-ga, ga(44). the major gas present were ga(1) and ga(8). data are discussed in relation to previous results obtained in p. coccineus seed as well as in the embryo-suspensor system. | 1986 | 16665010 |
| gibberellins in embryo-suspensor of phaseolus coccineus seeds at the heart stage of embryo development. | gibberellins (gas) in suspensors and embryos of phaseolus coccineus seeds at the heart stage of embryo development were analyzed by combined gas chromatography-mass spectrometry (gc-ms). from the suspensor four c(19)-gas, ga(1), ga(4), ga(5), ga(6), and one c(20) ga, ga(44), were identified. from the embryo, five c(19)-gas ga(1), ga(4), ga(5), ga(6), ga(60) and two c(20) gas, ga(19) and ga(44) were identified. the data, in relation to previous results, suggest a dependence of the embryo on the s ... | 1989 | 16667026 |
| an alpha-amylase inhibitor gene from phaseolus coccineus encodes a protein with potential for control of coffee berry borer (hypothenemus hampei). | plant alpha-amylase inhibitors are proteins found in several plants, and play a key role in natural defenses. in this study, a gene encoding an alpha-amylase inhibitor, named alphaai-pc1, was isolated from cotyledons of phaseolus coccineus. this inhibitor has an enhanced primary structure to p. vulgaris alpha-amylase inhibitors (alpha-ai1 and alpha-ai2). the alphaai-pc1 gene, constructed with the pha-l phytohemaglutinin promoter, was introduced into tobacco plants, with its expression in regener ... | 2006 | 16901522 |
| over-expression of a gibberellin 2-oxidase gene from phaseolus coccineus l. enhances gibberellin inactivation and induces dwarfism in solanum species. | gibberellins (gas) are endogenous hormones that play a predominant role in regulating plant stature by increasing cell division and elongation in stem internodes. the product of the ga 2-oxidase gene from phaseolus coccineus (pcga2ox1) inactivates c(19)-gas, including the bioactive gas ga(1 )and ga(4), by 2beta-hydroxylation, reducing the availability of these gas in plants. the pcga2ox1 gene was introduced into solanum melanocerasum and s. nigrum (solanaceae) by agrobacterium-mediated transform ... | 2008 | 17999064 |
| effect of a bowman-birk proteinase inhibitor from phaseolus coccineus on hypothenemus hampei gut proteinases in vitro. | the coffee berry borer, hypothenemus hampei (ferrari), is an important devastating coffee pest worldwide. both trypsin and chymotrypsin enzyme activities from h. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. a serine proteinase inhibitor belonging to the bowman-birk class was purified from a wild accession of phaseolus coccineus l. seeds. the inhibitor (pcbbi1) is a cysteine-rich protein that is heat-stable at alkaline ph. maldi-tof/ms analysis showed that pcbbi1 oc ... | 2007 | 18020416 |
| nondestructive inspection of phaseolus coccineus l. soya beans by use of near-infrared lasers. | we propose a nondestructive inspection method for the quantitative analysis of molds inside soya beans (phaseolus coccineus l.) that does not render the beans unfit for human consumption. three near-infrared lasers are used for multispectral transmission imaging of the beans. eigenvalue analysis and a least-squares method are used for estimation of the diffuse reflectance spectrum of each type of mold. the estimated spectra are used for determining the internal concentration of the molds inside ... | 1999 | 18319846 |
| activity of alpha-amylase inhibitors from phaseolus coccineus on digestive alpha-amylases of the coffee berry borer. | seeds of scarlet runner bean ( phaseolus coccineus l.) were analyzed for alpha-amylase inhibitor (alpha-ai) activity. through the use of polyclonal antibodies raised against pure alpha-ai-1 from common bean ( phaseolus vulgaris l.), typical alpha-alphaiota polypeptides (mr 14-18 kda) as well as a large polypeptide of mr 32000 da, usually referred to as "amylase inhibitor like", were detected. the inhibitor activity present in four accessions of p. coccineus was examined, both in semiquantitative ... | 2008 | 18321052 |
| cohnella phaseoli sp. nov., isolated from root nodules of phaseolus coccineus in spain, and emended description of the genus cohnella. | a bacterial strain designated gspc1 t was isolated from root nodules of phaseolus coccineus in segovia (spain). the 16s rrna gene sequence of this strain showed 95.9 and 94.7 % sequence similarity, respectively, with those of the type strains of cohnella hongkongensis and cohnella thermotolerans. strain gspc1 t presented phenotypic, chemotaxonomic and molecular differences with respect to cohnella species which indicated that it belonged to a different species. the isolate was a gram-positive, a ... | 2008 | 18676468 |
| a novel sialic acid-specific lectin from phaseolus coccineus seeds with potent antineoplastic and antifungal activities. | a novel lectin (pcl) with specificity towards sialic acid was purified from phaseolus coccineus l. (p. multiflorus willd) seeds using ion exchange chromatography on cm and deae-sepharose, and gel filtration on sephacryl s-200 column. pcl was a homodimer consisting of 29,831.265 da subunits as determined by gel filtration and ms. also, pcl was a non-metalloprotein and its n-terminal 23-amino acid sequence, atetsfsfqrlnlanlvlnkess, was determined. subsequently, mtt method, cell morphological analy ... | 2009 | 18757189 |
| identification of cis-regulatory sequences that activate transcription in the suspensor of plant embryos. | little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. we analyzed the upstream region of the scarlet runner bean (phaseolus coccineus) g564 gene to understand how genes are activated specifically within the suspensor during early embryo development. previously, we showed that the g564 upstream region has a block of tandem repeats, which contain a conserved 10-bp motif (gaaaag(c)/(t)gaa), a ... | 2009 | 19208800 |
| reactions of valonia and of halicystis to colloids. | from the results of these tests it is clear that both halicystis and valonia have a high degree of tolerance for animal peptone, and a very high degree of tolerance for animal proteose and for egg albumen. the products of bacterial growths fostered by these proteins have a deleterious effect upon both species of algae; but, if it were possible to prevent bacterial growth entirely and at the same time supply proper food, it is probable that halicystis and valonia would show normal growth indefini ... | 1933 | 19872750 |
| identification and functional analysis of pcna1 and pcna-like1 genes of phaseolus coccineus. | proliferating cell nuclear antigen (pcna) is an essential factor in dna replication and in many other processes in eukaryotic cells. genetic analysis of phaseolus coccineus showed the presence of at least two pcna-like genes in the runner bean genome. two pcna genes have previously been found in a few plant species including arabidopsis, tobacco, and maize. in these species, genes were nearly identical. two cdnas of p. coccineus pcna (pcpcna1 and pcpcna-like1) have been identified that differ di ... | 2010 | 20007687 |
| screening of pseudomonas and bacillus isolates for potential biocontrol of the damping-off of bean (phaseolus coccineus). | in this study fifteen isolates of identified pseudomonas fluorescens and bacillus subtilis were investigated for control of bean damping-off disease caused by rhizoctonia solani. in vitro, p. fluorescens p-6 and b. subtilis b-3 showed the most inhibitory zone in dual culture assay against r. solani. the growth of p-6 (4.5 x 10(8) cfu/ml) was significantly higher than in the other treatments. in greenhouse condition, all of the isolates effectively controlled damping-off on bean. p. fluorescens p ... | 2009 | 20222559 |
| new virulence groups in fusarium oxysporum f. sp. phaseoli: the expression of the gene coding for the transcription factor ftf1 correlates with virulence. | fusarium oxysporum f. sp. phaseoli strains isolated from runner bean plants showing fusarium wilt symptoms were characterized. the analysis of the genetic diversity of these strains and the comparison with strains formerly isolated from diseased common bean plants grown in the same region of spain indicated a close genetic similarity among them. pathogenicity assays carried out on runner bean plants showed virulence differences that allowed the classification of these strains into three groups: ... | 2011 | 21091181 |
| phaseolus immature embryo rescue technology. | predominant among the production constraints of the common bean phaseolus vulgaris are infestation of ascochyta blight, bean golden mosaic virus (bgmv), and bean fly. interbreeding with phaseolus -coccineus l. and/or phaseolus polyanthus greenm has been shown to provide p. vulgaris with greater resistance to these diseases. for interspecific crosses to be successful, it is important to use p. coccineus and p. polyanthus as female parents; this prevents rapid reversal to the recurrent parent p. v ... | 2011 | 21207266 |
| rapid plant identification using species- and group-specific primers targeting chloroplast dna. | plant identification is challenging when no morphologically assignable parts are available. there is a lack of broadly applicable methods for identifying plants in this situation, for example when roots grow in mixture and for decayed or semi-digested plant material. these difficulties have also impeded the progress made in ecological disciplines such as soil- and trophic ecology. here, a pcr-based approach is presented which allows identifying a variety of plant taxa commonly occurring in centr ... | 2012 | 22253728 |
| application of dl-beta-aminobutyric acid (baba) as a root drench to legumes inhibits the growth and reproduction of the pea aphid acyrthosiphon pisum (hemiptera: aphididae). | dl-beta-aminobutyric acid (baba) is a non-protein amino acid that is an effective inducer of resistance against a variety of plant pathogens. however, examples of baba-induced resistance against insect herbivores have not been reported. we applied baba as a soil drench to legumes and monitored its effects on the pea aphid acyrthosiphon pisum (harris). on tic bean (vicia faba var. minor), baba increased aphid mortality, caused a reduction in the mean relative growth rate of individual insects and ... | 2005 | 16197565 |
| phytohemagglutinin: transitory enhancement of growth in phaseolus and allium. | phytohemagglutinin (difco) stimulates germination and early seedling growth in phaseolus coccineus, but not in ph. vulgaris, the species from which the compound is extracted. the differences to the controls reach a maximum at day 8, then they disappear. root growth in allium cepa is enhanced by phytohemagglutinin during the first 12 days of treatment, but only at temperatures below 20°c. | 1972 | 24477998 |
| modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement. | sugar beet, beta vulgaris spp. vulgaris is a biennial long day plant with an obligate requirement for vernalization (prolonged exposure to low temperature). as a spring crop in temperate european climates, it is vulnerable to vernalization-induced premature bolting and flowering, resulting in reduced crop yield and quality. gibberellins (gas) play important roles in key physiological processes including stem elongation (bolting) and flowering and are, therefore, potential targets for controlling ... | 2009 | 18696248 |
| characterization of leafy cotyledon1-like during embryogenesis in theobroma cacao l. | theobroma cacao l., an economically important crop for developing countries, can be experimentally propagated by somatic embryogenesis. because of their potential roles in embryogenesis, a gene candidate strategy was initiated to find gene homologues of the members of the leafy cotyledon family of transcription factors. a homologue of the leafy cotyledon1-like gene, that encodes the hap 3 subunit of the ccaat box-binding factor, was found in the cocoa genome (tcl1l). the translated peptide share ... | 2008 | 18094994 |
| the level of jasmonic acid in arabidopsis thaliana and phaseolus coccineus plants under heavy metal stress. | the effect of heavy metal stress as a potent abiotic elicitor for triggering an accumulation of jasmonic acid (ja) was investigated. copper and cadmium in in vivo conditions induced accumulation of jasmonates in mature leaves of arabidopsis thaliana and in young and oldest phaseolus coccineus plants. the dynamics of jasmonate accumulation showed a biphasic character in both plants. in the first phase, after 7 (a. thaliana) or 14h (p. coccineus) of exposure to cu or cd, a rapid increase of ja lev ... | 2005 | 16425452 |
| hplc profile analysis of oleanene-glucuronides in several edible beans. | hplc analysis and yield of oleanene-glucuronide (og) was done on some commercially available edible beans: seeds of glycine max, glycine max cv. kuromame, phaseolus vulgaris cv. torosuku, phaseolus vulgaris cv. toramame, phaseolus vulgaris cv. taishokintoki, phaseolus coccineus cv. ooshirobana, phaseolus coccineus cv. murasakihanamame, vigna unguiculata cv. chuguro, vigna angularis cv. dainagon, arachis hypogaea, pisum sativum, and vicia faba. all the beans listed above have og, though in varyin ... | 1998 | 9571771 |
| n abundance of nodules as an indicator of n metabolism in n(2)-fixing plants. | this paper expands upon previous reports of (15)n elevation in nodules (compared to other tissues) of n(2)-fixing plants. n(2)-fixing nodules of glycine max (soybeans), vigna unguiculata (cowpea), phaseolus vulgaris (common bean), phaseolus coccineus (scarlet runner bean), prosopis glandulosa (mesquite), and olneya tesota (desert ironwood) were enriched in (15)n. nodules of vicia faba (fava beans), arachis hypogaea (peanut), trifolium pratense (red clover), pisum sativum (pea), lathyrus sativus ... | 1982 | 16662517 |
| covalent labelling of the nadph: protochlorophyllide oxidoreductase from etioplast membranes with [3h]n-phenylmaleimide. | [3h]n-phenylmaleimide has been used to covalently label in a specific manner the substrate-protected thiol groups of the enzyme protochlorophyllide reductase. in membrane preparations from oat (avena sativa) and runner-bean (phaseolus vulgaris) seedlings, two related peptides of mol.wts. 35000/37000 and 34000/35000 respectively and showing properties expected of the reductase have been identified, whereas the same technique with barley (hordeum vulgare) extracts resulted in labelling a single pe ... | 1981 | 7306063 |
| rooted leaves for physiological experiments. | rooted leaves provide convenient material for investigating various physiological processes. details are given for producing rooted leaves of species with tuberous roots, e.g. sweet potato (ipomoea batatas) and dahlia (dahlia variabilis) and species with tap roots, e.g. sugar beet (beta vulgaris) and runner bean (phaseolus coccineus). the storage root formed on a single sugar-beet leaf develops concentric cambia suggesting that their formation is not determined by particular leaves. | 1972 | 24481792 |
| leaf movements and their relationship with the lunisolar gravitational force. | observation of the diurnal ascent and descent of leaves of beans and other species, as well as experimental interventions into these movements, such as exposures to light at different times during the movement cycle, led to the concept of an endogenous 'clock' as a regulator of these oscillations. the physiological basis of leaf movement can be traced to processes that modulate cell volume in target tissues of the pulvinus and petiole. however, these elements of the leaf-movement process do not ... | 2015 | 26205177 |
| [peculiarities of circadian rhythms in plants from different geographical latitudes]. | 1 two species of plants (taraxacum arcticum and arnica angustifolia), collected in spitsbergen (geogr. latitude 76-80 degrees) exhibit endogenous circadian leaf movements but also movements with shorter periods. astragalus frigidus, a. alpinus and hedysarum hedysaroides, collected in arctic regions of continental europe, also show endogenous diurnal leaf movements. 2. in most of the species tested, there was no difference in the length of the free running periods of plants from arctic and centra ... | 1966 | 24557984 |
| identification of traumatin, a wound hormone, as 12-oxo-trans-10-dodecenoic acid. | 12-oxo-trans-10-dodecenoic acid (trans-10-oda) is an oxidation product of polyunsaturated fatty acids in plant tissues. the structural similarity of trans-10-oda and traumatic acid, a compound considered to be a wound hormone, suggested that trans-10-oda might be a precursor of traumatic acid. both trans-10-oda and traumatic acid were active in the wehnelt bean assay. the results were more consistent with trans-10-oda than with traumatic acid. cucumber (cucumis sativus l. var. national pickling) ... | 1979 | 16660762 |
| purification and characterization of an α-galactosidase from phaseolus coccineus seeds showing degrading capability on raffinose family oligosaccharides. | an acidic α-galactosidase (ec 3.2.1.22) designated as phaseolus coccineus seeds galactosidase (pcg) was purified from p. coccineus seeds using ion-exchange chromatography on deae- and cm-cellulose, q- and sp-sepharose and gel filtration on superdex 75. the molecular weight of pcg was 43 kda as judged by sds-page and gel filtration. two inner peptides of pcg were sequenced by maldi-tof-ms. the optimum ph and temperature was 3.0 and 70 °c, respectively but was stable up to 60 °c for 30 min. the en ... | 2013 | 23727589 |
| [the importance of circadian leaf movements for the precision of day-length measurement]. | 1. in glycine max, the circadian leaf movements are synchronized with the 24 hr period in 12:12 hrs light-dark cycles even with light intensities of only 0.6-0.8 lx. the normal phase-angle difference between light-dark cycle and the circadian rhythm is already nearly reached with intensities of 10 lx. 2. in phaseolus multiflorus, light with the intensities of moonlight, offered during the dark-period of 12:12 hrs light-dark cycles, is able to shift the phases of the circadian leaf-movement rhyth ... | 1969 | 24515819 |
| relationship between oxalate, oxalate oxidase activity, oxalate sensitivity, and white mold susceptibility in phaseolus coccineus. | abstract sclerotinia sclerotiorum is a necrotrophic pathogen that devastates the yields of numerous crop species, including beans. the disease in common bean and pea is referred to as white mold. we examined the relationship between oxalate, an established virulence factor of s. sclerotiorum, and partial white mold resistance of scarlet runner bean (phaseolus coccineus). p. coccineus genotypes pi 255956 ('mayan white runner') and pi 535278 (tars-046a) were more resistant than susceptible 'wolven ... | 2005 | 18943123 |
| endogenous gibberellin transport and biosynthesis in relation to geotropic induction of excised sunflower shoot-tips. | surgical experiments on helianthus annuus and phaseolus multiflorus seedlings involving the application of auxin and gibberellin to decapitated plants, suggested that internode extension growth occurs under the controlling influence of apically synthesised gibberellin rather than auxin. studies were made of diffusible gibberellins from sunflower apical buds in relation to geotropic stimulation. approximately ten times as much gibberellin was obtained from lower than from upper tissues of horizon ... | 1972 | 24477810 |
| control of plant leaf movements by the lunisolar tidal force. | investigations into the diurnal ascent and descent of leaves of beans and other species, as well as experimental interventions into these movements, such as exposures to light at different times during the movement cycle, led to the concept of an endogenous 'clock' as a regulator of these oscillations. the causal origin of leaf movement can be traced to processes that modulate cell volume in target tissues of the pulvinus and petiole. however, these elements of the leaf-movement process do not s ... | 2018 | 29373644 |
| dark-chilling and subsequent photo-activation modulate expression and induce reversible association of chloroplast lipoxygenase with thylakoid membrane in runner bean (phaseolus coccineus l.). | lipoxygenases (loxs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids. this reaction is the first step in biosynthesis of oxylipins, which play important and diverse roles in stress response. in this study, we identified four lox genes (pcloxa, b, c, d) in chilling-sensitive runner bean (phaseolus coccineus l.) plant and analyzed their expression patterns during long term dark-chilling (4 °c) stress and during day/night (21ºc/4 °c) temperature fl ... | 2018 | 29207281 |
| domestication genomics of the open-pollinated scarlet runner bean (phaseolus coccineus l.). | the runner bean is a legume species from mesoamerica closely related to common bean (phaseolus vulgaris). it is a perennial species, but it is usually cultivated in small-scale agriculture as an annual crop for its dry seeds and edible immature pods. unlike the common bean, p. coccineus has received little attention from a genetic standpoint. in this work we aim to (1) provide information about the domestication history and domestication events of p. coccineus; (2) examine the distribution and l ... | 2017 | 29187858 |
| the mitochondrial vdac of bean seeds recruits phosphatidylethanolamine lipids for its proper functioning. | the voltage-dependent anion-selective channel (vdac) is the main pathway for inorganic ions and metabolites through the mitochondrial outer membrane. studies recently demonstrated that membrane lipids regulate its function. it remains, however, unclear how this regulation takes place. in this study, we show that phospholipids are key regulators of phaseolus vdac function and, furthermore, that the salt concentration modulates this regulation. both selectivity and voltage dependence of phaseolus ... | 2017 | 28666835 |
| high level of nonsynonymous changes in common bean suggests that selection under domestication increased functional diversity at target traits. | crop species have been deeply affected by the domestication process, and there have been many efforts to identify selection signatures at the genome level. this knowledge will help geneticists to better understand the evolution of organisms, and at the same time, help breeders to implement successful breeding strategies. here, we focused on domestication in the mesoamerican gene pool of phaseolus vulgaris by sequencing 49 gene fragments from a sample of 45 p. vulgaris wild and domesticated acces ... | 2016 | 28111584 |
| three-dimensional visualization of the tubular-lamellar transformation of the internal plastid membrane network during runner bean chloroplast biogenesis. | chloroplast biogenesis is a complex process that is integrated with plant development, leading to fully differentiated and functionally mature plastids. in this work, we used electron tomography and confocal microscopy to reconstruct the process of structural membrane transformation during the etioplast-to-chloroplast transition in runner bean (phaseolus coccineus). during chloroplast development, the regular tubular network of paracrystalline prolamellar bodies (plbs) and the flattened porous m ... | 2016 | 27002023 |
| does methyl jasmonate modify the oxidative stress response in phaseolus coccineus treated with cu? | the contribution of methyl jasmonate (mj) as a signal molecule able to take part in the defense mechanism against copper (cu)-imposed oxidative stress was studied in the leaves and roots of runner bean (phaseolus coccineus) plants. roots of plants cultivated hydroponically were preincubated in mj (10µm) for 1h or 24h and subsequently exposed to cu (50µm) for 5h (short-term experiment) or 5 days (long-term experiment). enzymatic (activity of superoxide dismutase, sod; catalase, cat; ascorbate per ... | 2016 | 26629660 |
| a dimeric phaseolus coccineus lectin with anti-oxidative, anti-proliferative and cytokine-inducing activities. | in the present study, a dimeric glucosamine binding lectin, designated as chl, was purified from phaseolus coccineus l. var. albonanus bailey through three chromatographic steps. the molecular weight of chl was approximately 66kda. its hemagglutinating activity toward rabbit erythrocytes was dependent on carbohydrates, especially glucosamine, and was stable at temperatures between 20 and 70°c, and at ph between 1 and 13. intriguingly, further characterization showed that chl served as a potent a ... | 2015 | 26410813 |
| genetic diversity and population structure of an italian landrace of runner bean (phaseolus coccineus l.): inferences for its safeguard and on-farm conservation. | the landraces are considered important sources of valuable germplasm for breeding activities to face climatic changes as well as to satisfy the requirement of new varieties for marginal areas. runner bean (phaseolus coccineus l.) is one of the most cultivated phaseolus species worldwide, but few studies have been addressed to assess the genetic diversity and structure within and among landrace populations. in the present study, 20 different populations of a runner bean landrace from central ital ... | 2015 | 26036320 |
| a cis-regulatory module activating transcription in the suspensor contains five cis-regulatory elements. | little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. we analyzed the upstream region of the scarlet runner bean (phaseolus coccineus) g564 gene in order to understand how genes are activated specifically in the suspensor during early embryo development. previously, we showed that a 54-bp fragment of the g564 upstream region is sufficient for suspensor transcription and contains at least t ... | 2015 | 25796517 |
| using giant scarlet runner bean embryos to uncover regulatory networks controlling suspensor gene activity. | one of the major unsolved issues in plant development is understanding the regulatory networks that control the differential gene activity that is required for the specification and development of the two major embryonic regions, the embryo proper and suspensor. historically, the giant embryo of scarlet runner bean (srb), phaseolus coccineus, has been used as a model system to investigate the physiological events that occur early in embryogenesis-focusing on the question of what role the suspens ... | 2015 | 25705214 |
| evaluation of foliar phenols of 25 mexican varieties of common bean (phaseolus vulgaris l.) as antioxidants and varietal markers. | the antioxidant properties and the foliar phenol composition of 25 mexican varieties of phaseolus vulgaris l. (common bean) were evaluated. phaseolus coccineus was analysed with comparative aims. the high performance liquid chromatography with photodiode array detection analysis revealed 27 phenolics in the leaves of p. vulgaris (13 quercetin-3-o-glycosides, 8 kaempferol-3-o-glycosides, 2 myricetin glycosides and 4 phenolic acids) and 5 in p. coccineus (2 kaempferol-3-o-glycoside, 2 apigenin-7-o ... | 2014 | 24969366 |
| origin of ion selectivity in phaseolus coccineus mitochondrial vdac. | the mitochondrial voltage-dependent a nion-selective channel (vdac) is the major permeation pathway for small ions and metabolites. although a wealth of electrophysiological data has been obtained on different vdac species, the physical mechanisms of their ionic selectivity are still elusive. we addressed this issue using electrophysiological experiments performed on plant vdac. a simple macroscopic electrodiffusion model accounting for ion diffusion and for an effective fixed charge of the chan ... | 2014 | 24742372 |
| rapid characterisation and comparison of saponin profiles in the seeds of korean leguminous species using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (uplc-pda-esi/ms) analysis. | the present work was reported on investigation of saponin profiles in nine different legume seeds, including soybean, adzuki bean, cowpea, common bean, scarlet runner bean, lentil, chick pea, hyacinth bean, and broad bean using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (uplc-pda-esi/ms) technique. a total of twenty saponins were characterised under rapid and simple conditions within 15min by the 80% methanol extracts of a ... | 2014 | 24176342 |
| photoperiodic plasticity in circadian clock neurons in insects. | since bünning's observation of circadian rhythms and photoperiodism in the runner bean phaseolus multiflorus in 1936, many studies have shown that photoperiodism is based on the circadian clock system. in insects, involvement of circadian clock genes or neurons has been recently shown in the photoperiodic control of developmental arrests, diapause. photoperiod sets peaks of period (per) or timeless (tim) mrna abundance at lights-off in sarcophaga crassipalpis, chymomyza costata and protophormia ... | 2013 | 23986711 |
| structural and functional based identification of the bean (phaseolus) micrornas and their targets from expressed sequence tags. | micrornas (mirnas) are small, 18-26 nucleotides long, non-coding rnas that play role in post-transcriptional gene regulation. many of these are evolutionarily conserved. this suggests a powerful approach to predict new mirnas in other species. in this research, structural and functional approaches were combined to make computational prediction of potential mirnas and their targets in bean (phaseolus). total 55 novel mirnas were detected from 38 mirnas families in bean (phaseolus). these families ... | 2013 | 23605779 |
| european phaseolus coccineus l. landraces: population structure and adaptation, as revealed by cpssrs and phenotypic analyses. | relatively few studies have extensively analysed the genetic diversity of the runner bean through molecular markers. here, we used six chloroplast microsatellites (cpssrs) to investigate the cytoplasmic diversity of 331 european domesticated accessions of the scarlet runner bean (phaseolus coccineus l.), including the botanical varieties albiflorus, bicolor and coccineus, and a sample of 49 domesticated and wild accessions from mesoamerica. we further explored the pattern of diversity of the eur ... | 2013 | 23451209 |
| contrasting evolution of a satellite dna and its ancestral igs rdna in phaseolus (fabaceae). | cc4 is a satellite dna from common bean (phaseolus vulgaris l.) that is similar to its intergenic spacer (igs) rdna. cc4 was originally hypothesized to be an old, fast evolving satellite family that has invaded common bean rdna. to test this hypothesis and contribute to the understanding of igs-like satellite dna evolution, we have investigated its distribution in the genus phaseolus and related species. cc4 was cloned and used as probe for southern blot and fish experiments. cc4 was observed as ... | 2012 | 23050694 |
| novel vitamin e forms in leaves of kalanchoe daigremontiana and phaseolus coccineus. | in the present study, we isolated novel tocochromanols from green leaves of kalanchoe daigremontiana and primary leaves of etiolated seedlings of phaseolus coccineus that were identified as β-, γ-, and δ-tocomonoenols with unsaturation at the terminal isoprene unit of the side chain. the content of γ-tocomonoenol in leaves of etiolated bean increased gradually with the age of seedlings, reaching 50% of the γ-tocopherol level in 40-day-old plants. the content of this compound in leaves was increa ... | 2011 | 21856038 |
| biodiversity studies in phaseolus species by dna barcoding. | the potential of dna barcoding was tested as a system for studying genetic diversity and genetic traceability in bean germplasm. this technique was applied to several pure lines of phaseolus vulgaris l. belonging to wild, domesticated, and cultivated common beans, along with some accessions of phaseolus coccineus l., phaseolus lunatus l., and vigna unguiculata (l.) walp. a multilocus approach was exploited using three chloroplast genic regions (rbcl, trnl, and matk), four intergenic spacers (rpo ... | 2011 | 21777058 |
| genetic diversity and structure of a worldwide collection of phaseolus coccineus l. | phaseolus coccineus l. is closely related to p. vulgaris and is the third most important cultivated phaseolus species. little is known about the patterns of its diversity. in this work, a representative collection of its worldwide diversity was initially developed. the collection includes 28 wild forms (wfs) and 52 landraces (lrs) from mesoamerica (the crop domestication area), and 148 lrs from europe (where the crop was introduced in the sixteenth century). the collection was studied by using 1 ... | 2011 | 21279322 |
| modulation of plant mitochondrial vdac by phytosterols. | we have investigated the effect of cholesterol and two abundant phytosterols (sitosterol and stigmasterol) on the voltage-dependent anion-selective channel (vdac) purified from mitochondria of bean seeds (phaseolus coccineus). these sterols differ by the degree of freedom of their lateral chain. we show that vdac displays sensitivity to the lipid-sterol ratio and to the type of sterol found in the membrane. the main findings of this study are: 1), cholesterol and phytosterols modulate the select ... | 2010 | 20923643 |
| partial characterization of the lectin of runner beans (phaseolus coccieneus) var. alubia. | we extracted and partial characterized lectin from runner beans (phaseolus coccineus l.). this lectin shows a great affinity to fetuin-agarose column like others lectins and the electrophoretic gels point one band of approximately 45 kda. in addition to the previous assays, we detected the presence of lectins by agglutination assays. we know that lectins are non-enzymatic proteins or glycoproteins that bind carbohydrates. the biological function of plant lectins is not fully understood, but they ... | 2009 | 19579989 |
| digestive alpha-amylases from tecia solanivora larvae (lepidoptera: gelechiidae): response to ph, temperature and plant amylase inhibitors. | the biochemical properties of the digestive alpha-amylase from tecia solanivora larvae, an important and invasive insect pest of potato (solanum tuberosum), were studied. this insect has three major digestive alpha-amylases with isoelectric points 5.30, 5.70 and 5.98, respectively, which were separated using native and isoelectric focusing gels. the alpha-amylase activity has an optimum ph between 7.0 and 10.0 with a peak at ph 9.0. the enzymes are stable when heated to 50 degrees c and were inh ... | 2008 | 18590601 |
| gamma-tocopherol dominates in young leaves of runner bean (phaseolus coccineus) under a variety of growing conditions: the possible functions of gamma-tocopherol. | it has been shown that young leaves of runner bean (phaseolus coccineus) plants grown under natural conditions have an unusually high content of gamma-tocopherol, accounting for up to 90% of all tocopherols and 50% of the chlorophyll content. the level of gamma-tocopherol gradually decreased during the first two weeks of leaf development. the high content of gamma-tocopherol in young leaves was not significantly influenced by growth conditions. in contrast to seeds, gamma-tocopherol was also the ... | 2008 | 18582912 |
| tocopherol quinone content of green algae and higher plants revised by a new high-sensitive fluorescence detection method using hplc--effects of high light stress and senescence. | a rapid, sensitive fluorescence method was applied here for detection of oxidized tocopherol quinones in total plant tissue extracts using hplc, employing a post-column reduction of these compounds by a zn column. using this method, we were able to detect both alpha- and gamma-tocopherol quinones in chlamydomonas reinhardii with a very high degree of sensitivity. the levels of both compounds increased under high light stress in the presence of pyrazolate in parallel to a decrease in the content ... | 2008 | 18541333 |
| dna degradation during programmed cell death in phaseolus coccineus suspensor. | the suspensor of phaseolus coccineus l. degenerates at the cotyledonary stage of embryogenesis when it is no longer necessary for continued embryonic development; this degeneration is considered to be a typical example of the so-called developmental programmed cell death (pcd) in plants. the presence of specific hallmarks of pcd as it occurs during the degeneration of p. coccineus suspensor was investigated in the current study. by using the tunel assay and electrophoretic analysis, we found evi ... | 2016 | 17399991 |
| lipid peroxidation in cadmium-treated phaseolus coccineus plants. | lipid peroxidation was examined in phaseolus coccineus plants treated with 25 microm cadmium (cd) for 12 days at two different growth stages of the primary leaves. in the leaves of cd-treated young plants, the content of free fatty acids was higher only after 8 days of plant exposure to the metal, lipoxygenase activity at ph 7.0 was enhanced after 6 and 8 days of cd action, but at ph 8.0 it was higher than control, especially after 8 days. non-enzymatic lipid peroxidation was higher after 6 days ... | 2006 | 16418896 |
| contact urticaria from runner bean (phaseolus coccineus). | | 2002 | 12492534 |
| molecular research on the genetic diversity of polish varieties and landraces of phaseolus coccineus l. and phaseolus vulgaris l. using the rapd and aflp methods. | the aim of our research was to evaluate the genetic diversity among 25 commercial varieties registered in poland and 14 landraces of phaseolus vulgaris var. nanus asch. (the dwarf common bean) and phaseolus coccineus l. (the runner bean) maintained in the national centre of plant genetic resources in radzików. an additional goal of this study was to compare the precision and efficiency of two techniques of pcr (rapd and aflp), used to estimation the genetic diversity of bean. the breeding variet ... | 2002 | 12378235 |
| evidence for the direct 2beta- and 3beta-hydroxylation of [2h2]ga20-13-o-[6'-2h2]glucoside in seedlings of phaseolus coccineus. | [17-2h2]ga20-13-o-[6'-2h2]glucoside was synthesized and applied to seedlings of phaseolus coccineus l. after incubation for 72 h the conjugate metabolites were purified and shown by lc-esi-tandem-ms and gc-ms to be [17-2h2]ga1-13-o-[6'-2h2]glucoside and [17-2h2]ga29-13-o-[6'-2h2]glucoside. this is the first evidence for the conversion of intact ga-o-glucosides, and represents an additional metabolic pathway of the gibberellin metabolism in p. coccineus l. the results indicate that intact ga-o-gl ... | 2002 | 12354189 |
| primary structures of alpha- and beta-subunits of alpha-amylase inhibitors from seeds of three cultivars of phaseolus beans. | the primary structures of three alpha-amylase inhibitors (tai, dai, and mai-2) consisting of glycoprotein subunits alpha and beta from the respective seeds of three cultivars of phaseolus beans, toramame (phaseolus vulgaris l.), daifukumame (phaseolus vulgaris l.), and murasakihanamame (phaseolus coccineus l.) were determined by sequencing the peptide fragments derived from their enzymatic digestions. major sugar chains of the inhibitors were also assessed by analyzing glycopeptides in the enzym ... | 2002 | 11902670 |
| the contribution of internode and mesocotyl tissues to root-to-shoot signalling of abscisic acid. | the xylem of first internode of runner bean and of previously etiolated maize mesocotyl segments was perfused with media containing abscisic acid (aba) or abscisic acid glucose ester (aba-ge) in concentrations as they occur under stress conditions. aba-ge passed through the internode and mesocotyl segments unchanged. within 10 min the concentration of aba-ge(xyl) rose to a level similar to that in the external perfusion medium. by contrast, 30-40 min passed before the concentration of free aba i ... | 2002 | 11807133 |
| photosynthetic light response curves determined with the leaf oxygen electrode: minimisation of errors and significance of the convexity term. | from photosynthetic studies on a range of monocotyledonous (c-3 and c-4) and dicotyledonous (c-3) plants using a leaf oxygen electrode, we conclude the following. (i) a non-linear model [j.h.m. thornley (1976) mathematical models in plant physiology, academic press, london; b. marshall and p.v. biscoe (1980) j exp bot 31:29-39] significantly better describes the photosynthetic light response curve [rate of photosynthesis (p) versus incident photosynthetic photon flux density (i)] than the freque ... | 2001 | 11762163 |
| fluorescent in situ hybridization in plant polytene chromosomes. | polytene chromosomes are found in specialized tissues, with high metabolic activity, of a few angiosperm genera. they differ from diptera polytenics in several aspects, mainly because their chromatids on each chromosome are not tightly paired, nor are they so highly endoreplicated as those of diptera. in situ hybridization with isotopic and non-isotopic probes has been successfully used in plant polytene chromosomes, mainly in phaseolus coccineus and vigna unguiculata, where they have been best ... | 2001 | 11741150 |
| on the reversible conjugation of | after administering [17-d(2)]ga(20) to phaseolus coccineus l. cv. preisgewinner seedlings, [17-d(2)]ga(20)-o-glucoside was identified by liquid chromatography (lc)/esi-tandem mass spectrometry (ms). likewise, by lc/esi-tandem ms the metabolic formation of [17-d(2)]ga(20) glucosyl ester was established. the application of both [17-d(2)]-labeled ga(20) 13-o-glucoside and ga(20) glucosyl ester to phaseolus coccineus l. seedlings resulted in free [17-d(2)]ga(20) by gas chromatography/ms. the results ... | 1999 | 10552132 |
| a family of dispersed repeats in the genome of vicia faba: structure, chromosomal organization, redundancy modulation, and evolution. | a family of repeated dna sequences of about 1200 bp in length and bordered by well-conserved, 18 bp inverted repeats (vfb family) was found in the nuclear genome of vicia faba. the structure, chromosomal organization, redundancy modulation and evolution of these sequences were investigated. they are enriched in a+t base pairs (about 40% g+c) and lack any obvious internally repeated motif. a 64%-73% nucleotide sequence identity was found when pairwise comparisons between vfb sequences were carrie ... | 1999 | 10525968 |
| ribosomal rna genes of phaseolus coccineus. iv. structure and comparative-analysis of the intergenic spacer: possible involvement of some nucleotides in the transcription control of coding sequences. sequence of the intergenic spacer of ribosomal genes. | the sequence of the intergenic spacer (igs) of phaseolus coccineus is determined. the igs contains three distinct regions: region a, constant in length; region b, heterogeneous in length among genes, including two very similar segments 162 and 177 bp long, repeated two and nine times respectively in the investigated clone; region c, constant in length, comprising five islands. the putative promoters and the sites of termination, processing and methylation are detected by a comparison with other ... | 1998 | 9773275 |