| recognition at cell surfaces: phytohaemagglutinin-lymphocyte interaction. | many aspects of cell behaviour are regulated by the interaction of extracellular ligands with specific receptors exposed on the cell surface. the receptors correspond to membrane proteins and expecially glycoproteins. a key event in regulation is the transmission across the surface membrane of the information resulting from receptor-ligand interaction. the activation of lymphocytes by phaseolus vulgaris phytohaemagglutinin (pha) provides a convenient experimental model for the study of the molec ... | 1975 | 1813 |
| specific agglutination of escherichia coli o128b12 by the mannose-binding proteins of pseudomonas aeruginosa. | the mannosephilic haemagglutinins of pseudomonas aeruginosa were found to agglutinate cells of escherichia coli o128b12, to be adsorbed onto them and to attach peroxidase to them. these reactions were specifically inhibited by d-mannose. no agglutination by this pseudomonas haemagglutinin was obtained when several other enteropathogenic types of escherichia coli and some other gram-negative bacteria were examined. concanavalin a, which also reacted with escherichia coli o128b12 cells, interacted ... | 1977 | 25370 |
| the energy value to sheep of mixed diets containing alkali-treated straw and ground field beans [proceedings]. | | 1978 | 32540 |
| [comparative study of the antiviral properties of histones of animal and plant origin]. | the antiviral properties of histones of animal (thymus) and plant (french beans) origin were studied in plants and with a plant virus, tobacco mosaic virus (tmv). histones of the thymus and french beans were shown to be able to inhibit tmv reproduction. the antiviral properties of histones were found to depend on their concentration, ph, and to be determined by the modes of their introduction into leaves. the manifestation of the antiviral properties of histones seems to require not only their d ... | 1978 | 34928 |
| age-related refractoriness of pha-induced lymphocyte transformation. ii. 125-i-pha binding to spleen cells from young and old mice. | using 125-i-labelled red kidney bean phytohemagglutinin (125-i-pha), we have found that spleen cells from old bc3f1 mice bind this plant mitogen equally well, if not better, than spleen cells from young bc3f1 mice, although pha-induced blastogenesis of spleen cells from old mice is sharply reduced. analyes demonstrated that there is neither significant alteration of binding affinity nor decreased total number of membrane receptor sites for pha in senescing mouse spleen cells. the amount of pha w ... | 1975 | 50503 |
| specific interaction of human tamm-horsfall gylcoprotein with leucoagglutinin, a lectin from phaseolus vulgaris (red kidney bean). | human tamm-horsfall glycoprotein inhibits lymphocyte transformation induced by leucoagglutinin and haemagglutinin from phaseolus vulgaris (red kidney bean). the glycoprotein interacts with the two lectins, giving insoluble precipitates. the interaction with leucoagglutinin is highly specific, and the shape of the precipitin curve is that of an antigen-antibody reaction; precipitation is specifically inhibited by n-acetyl-d-galactosamine. results are discussed, and it is suggested that inhibition ... | 1979 | 118744 |
| relationship between enhanced turnover of phosphatidylinositol and lymphocyte activation by mitogens. | 1. various lectins [phaseolus vulgaris phytohaemagglutinin, glycine max (soy-bean) agglutinin, triticum vulgaris (wheat-germ) agglutinin and axinella polyploides agglutinin] and antibodies to pig ig (immunoglobulin) that are found by pig lymphocytes were assessed in terms of their capacities to stimulate lymphocyte transformation and to enhance phosphatidylinositol turnover. transformation was measured after 45h of culture by incorporation of [6-(3)h]thymidine into dna, whereas phosphatidylinosi ... | 1975 | 167716 |
| thrombin-induced protein phosphorylation in human platelets. | intact human platelets loaded with 32po4 contain multiple phosphorylated proteins. thrombin treatment of intact 32po4-loaded platelets results in a 2-6-fold increase in phosphorylation of a platelet protein (designated "peak 7" protein) of approximately 40,000 mol wt as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by gel filtration on sephadex g-150. a similar increase in phosphorylation was observed in a platelet protein (designated "peak 9" protein) of approximat ... | 1975 | 169298 |
| surface alterations in calf lymphocytes oxidized by sodium periodate. | in order to investigate alterations in surface structure in transformed lymphocytes, calf submandibular lymph node cell suspensions were oxidized with naio4. oxidezed lymphocytes were morphologically transformed and had higher rates of dna synthesis by 2 days after treatment. these results were prevented by reduction of the cell suspension with nabh4, or by neuraminidase treatment of cells prior to oxidation. the amount of 125i-labeled agaricus bisporus lectin bound to cells immediately after ox ... | 1976 | 178647 |
| effects of concanavalin a on 5-hydroxytryptamine uptake by rabbit blood platelets and on their ultrastructure. | 1. effects of concanavalin a (con a) and other lectins on 5-hydroxytryptamine (5-ht) uptake by rabbit blood platelets and on their ultrastructure were studied. 2. uptake of [3h]-5-ht by platelets was decreased by application of con a, e-pha (lectin from phaseolus vulgaris) and lentil-pha (lectin from lens culinaris), but not by wheat germ agglutinin (wga). con a induced specific changes in the ultrastructure of platelets, causing (i) a change in external appearance from a discoid to an irregular ... | 1979 | 219926 |
| [effects of a phytohemagglutinin extract on growth, nitrogen digestibility and the activity of invertase and (na+-k+)-atpase in the intestinal mucosa of the rat]. | a phytohemagglutinin extract is prepared from raw kidney beans (phaseolus vulgaris) and incorporated at a level of 1% (dry matter) in the diet of young growing rats. beside a decrease of feed intakes, the main effects of the experimental diet are the following : growth depression, decrease of dry matter and protein digestibility and hypoglycemia. biological value, organs weight (liver, kidneys, spleen) did not change significantly. the hemagglutinin extract induces an inhibition of saccharase ac ... | 1979 | 231410 |
| purification and characterization of guinea pig lymphotoxin produced by lymph node cells stimulated by phytohemagglutinin. | guinea pig lymphotoxin (lt) produced by stimulation of lymph node cells with phaseolus vulgaris phytohemagglutinin was purified approximately 1,000-fold in specific activity by ammonium sulfate fractionation, deae-sephadex column chromatography, and gel filtration on sephadex g-100. the properties of lt tested at the various stages of purification revealed that the lt was a heat-sensitive, protease-sensitive proteinaceous substance, and its approximate molecular weight was estimated to be 50,000 ... | 1975 | 239474 |
| purification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, phaseolus vulgaris. | kidney beans, phaseolus vulgaris, contain a proteinaceous inhibitor of alpha-amylase, which we have named phaseolamin. the inhibitor has been purified to homogeneity by conventional protein fractionation methods involving heat treatment, dialysis, and chromatography on deae-cellulose, sephadex g-100, and cm-cellulose. phaseolamin is specific for animal alpha-amylases, having no activity towards the corresponding plant, bacterial, and fungal enzymes, or any other hydrolytic enzyme tested. optimal ... | 1975 | 240849 |
| proteinaceous alpha-amylase inhibitor from beans (phaseolus vulgaris). purification and partial characterization. | | 1978 | 309845 |
| interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells. | the interaction of peanut agglutinin (pna) with human thymocytes, peripheral blood lymphocytes, and peripheral blood cells of various types of leukemia was investigated by using fluorescein isothiocyanate-conjugated pna. the majority of human thymocytes (60-80%) bind the lectin. the major subpopulation of thymocytes that is pna-positive was separated from the pna-negative cells by differential agglutination with the lectin. the two thymocyte subpopulations were tested in the mixed lymphocyte rea ... | 1979 | 311473 |
| surface glycoproteins from human t, b and chronic lymphatic leukemia lymphocytes isolated by 3 different lectins. | glycoproteins from the surface of human t. b and leukemic lymphocytes were isolated and partially characterized by using three different lectins, lens culinaris lectin (lc), wheat germ agglutinin (wga) and phytohemagglutinin from phaseolus vulgaris (pha). cells were labelled either with i125 or 3h-fucose and lysed with detergent. labelled components were isolated by incubation with lectins and anti-lectin antisera and immune precipitates were analysed by sds-polyacrylamide gel electrophoresis. t ... | 1979 | 316713 |
| type a botulism from commercially canned beef stew. | two of three persons who ate lunch together became ill with symptoms characteristic of botulism. one died before botulism was suspected and before specimens could be collected for laboratory testing, but a serum specimen from the other patient, who survived, yielded botulinal toxin, type a. the third person remained asymptomatic, but clostridium botulinum type a was cultured from his stool. the three persons had shared two canned foods: home-canned green beans and commercially canned beef stew. ... | 1977 | 320672 |
| mitogenic receptors on human peripheral blood lymphocytes: the interaction of phaseolus vulgaris erythroagglutinating phytohemagglutinin and anti-thymocyte globulin on the human peripheral blood lymphocyte membrane. | a horse anti-human thymocyte antibody (atg) obtained from the upjohn company was shown to stimulate dna synthesis in human lymphocytes with a time course and magnitude of radioactive thymidine uptake comparable to that seen with phytohemagglutinin (e-pha) and concanavalin a (con a). low mitogenic or nonmitogenic concentrations of intact atg or its fab fragments inhibited e-pha-induced mitogenesis, whereas the response to con a was unaffected. competitive binding studies with atg and e-pha reveal ... | 1977 | 325143 |
| properties of washed human platelets. | we have shown previously that washed human platelets resuspended in tyrode solution containing albumin and apyrase maintain their disc shape and their ability to aggregate upon the addition of low concentration of adp, providing fibrinogen is added to the suspending medium. we have now examined their responses to other aggregating and release-inducing agents. collagen, arachidonate, thrombin, immune serum globulin, the ionophore a23, 187 and phytohaemagglutinin from phaseolus vulgaris caused agg ... | 1977 | 327607 |
| improved detection of coliforms and escherichia coli in foods by a membrane filter method. | analytical procedures based on filtration of homogenates through membrane filters, and particularly hydrophobic grid-membrane filters (hgmf), offer definite improvements in the enumeration of escherichia coli and coliforms in foods. whereas the counted specimen in pour plates may not usually be greater than 0.1 g, up to 1.0 g of ground beef, green beans, potato, cod, strawberries, or grapes could be filtered and counted on hgmf. greatly improved limit of detection, reduced interference by noncol ... | 1979 | 394679 |
| prevention of sulfhydryl autoxidation by a polypeptide from red kidney beans, described to be a stimulator of rna synthesis. | | 1977 | 408159 |
| interaction of trypsin-like protease from streptomyces griseus with an immobilized inhibitor from kidney bean. | an immobilized double-headed inhibitor from phaseolus vulgaris l. selectively binds the trypsin-like enzyme produced by streptomyces griseus. binding takes place at ph 8.0, and at ph 2.0 the protease can be quantitatively released from the complex. purified by affinity chromatography, the trypsin-like enzyme is homogeneous according to polyacrylamide gel electrophoresis and ultracentrifugation data. physico-chemical and enzymic properties of the enzyme are identical to those exhibited by the enz ... | 1978 | 413581 |
| influence of polyethylene glycol and related compounds on the nutritional availability of methionine in a high-tannin sorghum and in field beans. | | 1977 | 414230 |
| nutritional effects of field bean (vicia faba l.) proteinase inhibitors fed to rats. | 1. weanling rats were fed on purified-casein diets containing active and inactive (autoclaved) field bean (vicia faba l.) proteinase inhibitors (pi) at levels of 1.25, 2.5, 5.0 and 10 g/kg. diets containing raw- and autoclaved-field-bean meal (fbm) were also given. the body-weight gain, protein efficiency ratio (per); i.e. the ratio of the live weight gain in g/g of protein consumed and apparent nitrogen digestibility were determined 7, 14 and 21 d after the start of the trial. 2. significant gr ... | 1979 | 420757 |
| effects of dietary proteinase inhibitors from field bean (vicia faba l.) and field-bean meal on pancreatic function in the rat. | | 1979 | 420758 |
| protein quality in cereals and pulses. 1. application of microbiological and other in vitro methods in the evaluation of rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). | 1. the streptococcus zymogenes assay procedure was modified and used in the estimation of available methionine and relative nutritional value in rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). the results were assessed in relation to the content of tannins and 'dye-binding lysine', and to published information on the nutritional quality of some of the test samples. 2. in grain of ten varieties of sorghum, for which other workers had reported a cor ... | 1979 | 427087 |
| the nutritional toxicity of phaseolus vulgaris lectins. | in rats fed on beans (phaseolus vulgaris) the poorly digestible lectins were shown to react with intestinal cells in vivo and to cause a disruption of many of the brush borders of duodenal and jejunal enterocytes. although depressed to a certain extent, absorption still occurred, probably through the non-disrupted cells of the small intestine. in addition, abnormal absorption of potentially harmful substances, lectin-related or of bacterial origin, could also occur, possibly as a direct effect o ... | 1979 | 461432 |
| protein quality in cereals and pulses. 2. influence of polyethyleneglycol on the nutritional availability of methionine in sorghum (sorghum vulgar pers.), field beans (vicia faba l.) and barley. | 1. polyethyleneglycol (peg 4000) was examined for its influence on relative nutritional value (rnv) and available methionine in sorghum (sorghum vulgare pers.), field beans (vicia faba l.) and barley, as measured microbiologically with streptococcus zymogenes. the results were assessed in relation to the content of tannins in the test samples. 2. in grain of hybrid sorghum the rnv averaged 87 (range 79--92) for six low-tannin varieties and 41 (30--53) for eleven high-tannin varieties. the corres ... | 1979 | 476044 |
| protein quality in cereals and pulses. 3. bioassays with rats and chickens on sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). influence of polyethylene glycol on digestibility on the protein in high-tannin grain. | 1. two preceding papers in this series describe the application of microbiological and other in vitro tests in the evaluation of sorghum (sorghum vulgare pers.), field beans (vicia faba l.) and barley, and in assessing the influence of polyethylene glycol (peg 4000) on the nutritional availability of the methionine. the present paper gives for comparison the results of bioassays on some of the same test samples. net protein utilization (npu) in rats was measured by the nitrogen balance method, a ... | 1979 | 476045 |
| varietal variation in the anti-nutritive effects of field beans (vicia faba) when fed to rats. | | 1979 | 491546 |
| nutritional evaluation of kidney beans (phaseolus vulgaris): chemical composition, lectin content and nutritional value of selected cultivars. | | 1979 | 502480 |
| digestibility of raw and cooked starches from legume seeds using the laboratory rat. | the in vivo digestibility and growth responses to diets containing raw and cooked legume starches were determined using male wistar rats. these starches were obtained from seven legumes including smooth- and wrinkled-seeded field peas, navy beans, kidney beans, garbanzo beans, mung beans and lentils. wheat and potato starch diets were fed for comparative purposes. with the exception of potato and the field pea starches, rats fed uncooked starch diets had higher weight gains than those fed cooked ... | 1979 | 512695 |
| age-related cell surface changes in human diploid fibroblasts revealed by lectin-mediated red blood cell adsorption assay: a lectin survey. | two types of age-related cell surface changes could be demonstrated in human diploid fibroblasts with the two methods of the lectin-mediated red blood cell (rbc) adsorption assay: the fibroblast coating method (in which rbcs are adsorbed to lectin-coated fibroblasts) and the rbc coating method (in which lectin-coated rbcs are adsorbed to fibroblasts). with the fibroblast coating method, concanavalin a and agglutinin l from phaseolus vulgaris gave a change in rbc adsorption which did not occur th ... | 1979 | 522510 |
| nutritive value of two different beans (phaseolus vulgaris) supplemented with methionine. | | 1979 | 546958 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| [examination and composition of some legume seeds (author's transl)]. | the chemical composition of some legume seeds is reviewed. it was found that soy (glycine soja), field beans (vicia faba), vicia sativa and lens culingris (red seeds) have the highest protein content. there were only minor differences in amino acid composition except for glutamic acid. the lysine content of legume seeds was high. lysine is the most limiting essential amino acid in cereal proteins, therefore cereal products can be improved nutritionally by addition of legume seed flour. examinati ... | 1977 | 560092 |
| effects of wheat germ agglutinin on membrane transport. | (1) low concentrations of wheat germ agglutinin are cytotoxic toward several tissue culture lines, including chinese hamster ovary cells, swiss 3t3 cells, mouse l cells and baby hamster kidney cells. the ld50 ranged from 1 to 5 microgram wheat germ agglutinin per ml. similar concentrations of the lectin inhibited the transport of the non-utilizable amino acids alpha-aminoisobutyric acid and cycloleucine and inhibited the uptake of thymidine. in contrast, 2-deoxy-d-glucose uptake was not altered ... | 1977 | 561616 |
| effects of lectins on the hemolysis of rabbit erythrocytes by straphylococcal alpha toxin. | when concanavalin a (1 microgram/ml) or wheat germ agglutinin (2 microgram/ml) was preincubated with a suspension of 2% rabbit erythrocytes for 5 min at 20 c, the binding [125i]-labeled staphylococcal alpha toxin to these erythrocytes was greatly inhibited and the hemolytic action of alpha toxin was decreased. the inhibitory effect of concanavalin a on hemolysis by alpha toxin was completely reversed in the presence of 0.1 m alpha-methyl-d-glucoside or alpha-methyl-d-mannoside. phytohemagglutini ... | 1977 | 563506 |
| certain environmental factors affecting rhizobia and symbiotic systems. | the interrelation between rhizobia and certain fungi, bacteria, actinomycetes, nematodes, and seed-coat diffusates of phaseolus vulgaris were investigated. the effect of pesticides, i.e. fungicides, herbicides, and nematocides on growth of rhizobia, and the symbiotic systems between rhizobia and their respective host is reported. degradation of certain herbicides and insecticides is shown. the movement of rhizobia in soil as affected by water tension, tolerance of salts, and soil temperatures ar ... | 1977 | 576107 |
| immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on platelet plasma membranes. | platelet plasma membranes were found to possess the disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine which was measured by gas chromatography after release by alkaline borohyride treatment and desialylation. immunological evidence using the specific lectins from arachis hypogoea and agaricus bisporus and an anti-t serum confirmed the presence of this disaccharide, the immunodominant group of the thomsen-friedenreich antigen (t-antigen). this receptor was only found after prior neura ... | 1978 | 580492 |
| the isolation and characterization of a lethal protein from kintoki beans (phaseolus vulgaris). | a lethal protein with hemagglutinating activity but without trypsin inhibitory activity was isolated from beans of phaseolus vulgaris, cultiva, and kintoki and proved homogeneous by ultracentrifugation, disc polyacrylamide gel electrophoresis, sodium dodesyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. the molecular weight was estimated to be 104, 000 by ultracentrifugal analysis and gel filtration on sephadex g-200. the molecule dissociates into three identical subunits ... | 1977 | 611161 |
| studies of the poor utilization by the rat of methionine and cystine in heated dry bean seed (phaseolus vulgaris). | | 1978 | 670563 |
| infection of phaseolus vulgaris by bean golden mosaic virus: ultrastructural aspects. | | 1978 | 685179 |
| [mode of action and inhibition of polygalacturonase covalently bound to polysaccharide and glass carriers]. | endo-polygalacturonase (ec 3.2.1.15.) from aspergillus spec. is much changed as far as its mode of action and the interaction with vegetable inhibitors of pectinase (from green beans and cucumbers) are concerned when it is covalently bound to insoluble carriers (sepharose, cellulose powder, macroporous glass and nonporous ballotinis). whereas a 2% degradation of substrate by the soluble enzyme caused a 50% decrease of viscosity of citrus pectic acid, the comparable degradation of substrate was i ... | 1978 | 692641 |
| nodule infection by bean yellow mosaic virus in phaseolus vulgaris. | infection of root nodules of beans, phaseolus vulgaris l., by bean yellow mosaic virus (bymv) and the effect of the disease on the specific activity of the nodule are reported. infectivity and serological microprecipitin assays with two sources of bymv antiserum demonstrated that nodules from bean plants whose leaves had been inoculated with bymv contain bymv antigen. the disease reduced the fresh weights of tops, roots, and root nodules and induced premature nodule decay and/or nodule drop. the ... | 1978 | 736539 |
| [nutritional evaluation of protein concentrates of beans (phaseolus vulgaris) and lentils (lens esculenta)]. | the composition and nutritive value were determined in navy bean meal (phaseolus vulgaris) and lentil meal (lens esculenta), and in their respective protein concentrates obtained through extraction followed by isoelectric precipitation. sulfur amino acids per gram of nitrogen were lower in the concentrates than in the meals, while there was no difference for lysine and threonine. the white bean protein concentrate had a lower biological value than the meal but better digestibility, although tryp ... | 1978 | 753176 |
| purification and base composition of a chloroplastic tranphe from phaseolus vulgaris. | | 1976 | 770194 |
| the interaction of nonmitogenic and mitogenic lectins with t lymphocytes: association of cellular receptor sites. | the relationship between the surface receptors on neuraminidase-treated human blood lymphocytes for the mitogenic lectins phaseolus vulgaris leukoagglutinin (la), concanavalin a (con a) and soy bean agglutinin (sba) and the non-mitogenic lectin helix pomatia a hemagglutinin (hp) was investigated. two different techniques, co-capping with different fluorochrome-labeled lectins and cell binding-inhibition experiments with 125i-labeled lectins, were used. the results demonstrated that the nonmitoge ... | 1976 | 772792 |
| immunogenic properties of eukaryotic ribosomal proteins. | pure rat, chicken and kidney bean ribosomal proteins are immunogenic in the rabbit as tested by the ouchterlony double diffusion technique. on the other hand, we were unable to raise antibodies to rabbit ribosomal proteins in the guinea pig. rabbit ribosomal proteins are not autoimmunogenic. rabbit antisera to rat or chicken ribosomal proteins surprisingly give no cross-reaction with ribosomal proteins from other mammals (man, ewe, calf, guinea pig, mice) whereas antisera to kidney bean ribosoma ... | 1975 | 804929 |
| synthesis of erythrocyte-specific proteins in cultured friend leukemia cells. | we have studied synthesis of specific proteins in two permanent lines of friend virus-induced erythroleukemia cells (friend line 745 and ostertag line fsd-1, both derived from dba/2 mice). by 96 hr following treatment with 1-2% dimethyl sulfoxide (me2so), up to 25% of the protein being synthesized by both these cultures is hemoglobin. at that time, hemoglobin constitutes up to 10% of the cellular soluble protein. both lines synthesize heme and globin coordinately, and alpha and beta globin chain ... | 1975 | 807334 |
| the amino-acid sequence of leghemoglobin component a from phaseolus vulgaris (kidney bean). | 1. leghemoglobin component a from phaseolus vulgaris (kidney bean) was digested with trypsin; 15 tryptic peptides and free lysine were purified and the amino acid sequences of the peptides determined. 2. the internal order of the tryptic peptides was determined by the bridge peptides obtained from the thermolytic digest and the dilute acid hydrolyzate of kidney bean leghemoglobin a; 12 thermolytic peptides and two acid hydrolysis peptides were purified and the sequences were partially or complet ... | 1975 | 809270 |
| immunoadsorbent affinity purification of the two enzyme forms of alpha-mannosidase from phaseolus vulgaris. | | 1976 | 826414 |
| the structure of the major protein of the human erythrocyte membrane. characterization of the intact protein and major fragments. | polypeptide 3, the major membrane-penetrating protein of the human erythrocyte membrane, was characterized, together with two major fragments derived by specific proteolysis of the native protein in the membrane. one fragment (fragment 3f) was obtained from thermolysin cleavage in the extracellular region of the protein, and the other (fragment t1) was derived from tryptic cleavage in the intracellular region of the protein. the results of n- and c-terminal group analysis suggest that fragment 3 ... | 1977 | 851416 |
| effects of cephalothin and penicillin g on platelet function in vitro. | high concentrations of cephalothin or penicillin g inhibit a number of the functions of human or rabbit platelets in citrated platelet-rich plasma (prp) and in suspensions of washed platelets. the reactions shown to be inhibited are: adp-induced shape change and the primary and secondary phases of aggregation and release induced by adp or adrenaline in human cirtated prp; release and aggregation of washed human platelets exposed to collagen, thrombin, vasopressin, or the ionophore a 23,187; aggr ... | 1977 | 869992 |
| attempts to overcome anti-nutritive factors in field beans (vicia faba l) and field peas (pisum sativum) fed in diets to laying hens. | | 1977 | 905287 |
| [protein quality and hemagglutin content in tender and dried bean seeds (phaseolus vulgaris) coscorrón variety]. | protein quality and hemagglutinating content in tender and dry seed beans: the chemical composition, hemagglutinating activity, protein queality (npu10) and true digestibility of samples at three certified bean seeds (phaseolus vulgaris) var coscorrón, tender and dry (recollected 90-95 days and 120-125 days after seeding respectively), were determined. the tender and dry seeds were submitted to usual cooking (tender equals 30' boiling and dry equals 90' boiling previously soaked for +/- 14 hours ... | 1977 | 907424 |
| studies on the rhizosphere and rhizoplane microflora of common-bean and barley. | both broad bean and barley plants stimulated bacteria (total), n-fixing clostridia, azotobacter ammonifiers, and aerobic cellulose-decomposers in their rhizosphere zone. the rhizosphere effects were generally positive until the 8th week of either plant growth. however, the rhizosphere effect differed according to type of plant, growth phase of each single plant, and type of micro-organism under study. the presence of high numbers of micro-organisms in the rhizoplane samples (washed ruptured root ... | 1977 | 910574 |
| [amino acid composition of the unresorbed peptide fractions of the distal part of the small intestine in rats. ii. feeding of field-bean protein isolate with and without the addition of dialdehyde starch and of protein texturates from it]. | rats were fed diets containing 20% protein in the form of casein, protein isolate from sunflower seeds, protein isolate from field beans, model protein texturate made from field-bean protein isolate or model protein texturate made from field-bean protein isolate treated with dialdehyde starch and a nitrogenfree diet. the content in the distal small intestine of the rats was collected 3 hours after feeding, and the amino-acid compositions of the tca-precipitable and tca-soluble peptides (after se ... | 1977 | 927479 |
| immunochemical studies of infectious mononucleosis. v. isolation and characterization of a glycoprotein from goat erythrocyte membranes. | a glycoprotein was isolated from goat erythrocyte membranes by extraction with hot 75% ethanol. the glycoprotein was purified by ethanol precipitation, phosphocellulose chromatography gel filtration, ethyl:ether and chloroform:methanol extraction. in aqueous phosphate-buffered saline, ph 7, the glycoprotein was in an aggregated state with a sedimentation coefficient (s(obs)) to 1.5. electrophoresis of the glycoprotein on polyacrylamide gels containing phosphate-buffered 0.1% sds gave a single ba ... | 1976 | 956650 |
| [nutritional study of field bean protein isolate and of spun field bean protein-casein fibers]. | the amino acid composition of the different field bean protein isolates shows a good correspondence. in relation to casein the content of the whole essential amino acids is low. in the first line the low content of methionine and cystine limits the biological value of this proteins. the content of lysine is relatively high. the enzymatic in vitro-hydrolysis results in a corresponding availability of the amino acids between the field bean proteins and casein. the digestibility is very good, the b ... | 1976 | 958366 |
| nutritional effects of field-bean (vicia faba l.) protease inhibitors and field beans fed to rats [proceedings]. | | 1976 | 972900 |
| comparative studies on some leguminous protein sources and soybean proteins. | the nutrient contents as well as the amino acid pattern of broad bean, kidney bean and soybean were determined. the levels of most of the amino acids were also estimated. heat treatment of the leguminous seeds caused a noticeable reduction in the levels of methionine, cystine, lysine and tryptophan. animal experiments showed high figures for digestibility, biological value and net protein utilization for the soybean diet whereas the broad bean and kidney beans gave lower values of these measurem ... | 1976 | 987657 |
| hybridization of bean chloroplast transfer rnas to chloroplast dna. | bean (phaseolus vulgaris) chloroplast trnasleu and trnasphe hybridize to chloroplast dna, whereas the corresponding cytoplasmic trna species do not, suggesting that chloroplast transfer rnas are coded for by chloroplast dna. the hybridization of the three chloroplast trnasleu or of the two trnasphe isoacceptors is not additive, and the isoacceptors compete with each other in the hybridization to chloroplast dna, suggesting that these isoacceptors are coded for by the same gene(s) and differ only ... | 1976 | 999912 |
| [evaluation of the quality of proteins of various vegetable grains used in various biological methods]. | the protein value of different legume foods was studied in weanling rats by determining net protein utilization (npu), protein efficiency ratio (per), biological value (bv), nitrogen balance (nb) and digestibility of the protein. npu was determined by carcass analysis and by nitrogen determination in one of the hind legs of the experimental animals. all samples were evaluated at a 10% protein level in the ration, with and without a methionine suplement. the effect of protein concentration in the ... | 1976 | 1008644 |
| a preparation of field bean (vicia faba l.) cotyledons as a substitute for dried skim-milk in calf feeding [proceedings]. | | 1976 | 1028050 |
| metabolic behaviour of water-soluble fluorescent whitening agents in the rat and bean plant. | the behaviour of three representative water-soluble fluorescent whitening agents (fwas) was studied in rats and bean plants using 14c-labelled compounds. following oral doses of 5 mg/kg of each of the fwas to rats, rapid and complete excretion of radioactive material was observed, with an excretion half life ranging from 7-13 hours. faeces were practically the only route of excretion, indicating, in combination with the short half life times, that no significant amounts of whitener were absorbed ... | 1975 | 1064534 |
| immunosuppressive activity of phaseolus coccineus and phaseolus vulgaris extracts in mice. | lectin mixtures from 10 samples of phaseolus coccineus and phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (srbc) antibody response of the mouse. some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin a and phytohemagglutinin tested before in the same immune system. high dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the t ... | 1976 | 1086791 |
| fractionation of human t lymphocytes on wheat germ agglutinin-sepharose. | t cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. when assayed with 125i- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (wga), a weakly binding and a strongly binding subpopulation could be distinguished. these t-cell subpopulations were fractionated on columns charged with wga, convalently bound to sepharose 6mb. the cells responding to the mitogens leukoagglutinin from phaseolus vu ... | 1976 | 1086886 |
| immunochemical and chemical investigations of the structure of glycoprotein fragments obtained from epiglycanin, a glycoprotein at the surface of the ta3-ha cancer cell. | the structures of the carbohydrate chains present in fragments of a large-molecular-weight glycoprotein, epiglycanin, cleaved from the surface of viable ta3-ha murine mammary carcinoma ascites cells and purified by gel filtration, were studied by immunochemical and chemical methods. inhibitory activities for neuraminidase-treated and untreated glycoprotein material in the hemagglutination of nn-specific human erythrocytes by eight purified lectins were determined. excellent inhibition was obtain ... | 1975 | 1125949 |
| nutritional evaluation of kidney beans (phaseolus vulgaris): the isolation and partial characterisation of toxic constituents. | | 1975 | 1134056 |
| a crystalline protein-proteinase inhibitor from pinto bean seeds. | a crystalline protein-proteinase inhibitor has been isolated from seeds of pinto bean (phaseolus vulgaris cultvar. pinto). it has an average molecular weight of 19 000 as estimated by gel filtration. this crystalline inhibitor is highly active against both bovine pancreatic trypsin and alpha-chymotrypsin. complexes of both trypsin-inhibitor and alpha-chymotrypsin-inhibitor have been isolated. the inhibitor which was derived from the dissociated trypsin-inhibitor complex was only 62% as effective ... | 1975 | 1148227 |
| [cooking of beans (phaseolus vulgaris)]. | | 1975 | 1164139 |
| collaborative study of a spectrophotometric method for determining maleic hydrazide residues in tobacco and vegetables. | a distillation-spectrophotometric method for the determination of maleic hydrazide residues in tobacco and vegetables reported previously was studied collaboratively. ten laboratories submitted analytical results on 10 samples containing residues resulting either from field treatment or laboratory fortification. the samples were estimated to contain from 0.99 to 16.36 mug maleic hydrazide/0.5 g sample analyzed. the coefficients of variation based on precision standard deviation ranged from 57.7% ... | 1975 | 1194188 |
| field-bean (vicia faba l.) protein in feeds for preruminant calves. | | 1975 | 1208478 |
| isolation and partial characterization of the major glycoproteins of horse and swine erythrocyte membranes. | the major glycoproteins of horse and swine erythrocyte membranes were isolated and examined chemically and immunologically. the major glycoprotein of horse erythrocyte membranes had a molecular weight of 33 000 and consisted of 46.2% protein and 53.8% carbohydrate, of which 9.4% was hexose, 10.1% hexosamine and 33.7% sialic acid. this glycoprotein was associated with activity for the infectious mononucleosis heterophile antigen. there were two different major glycoproteins in swine erythrocyte m ... | 1975 | 1238114 |
| [systemic infestation by cucumber mosaic virus on french bean (phaseolus vulgaris l.) in the german democratic republic (author's transl)]. | | 1975 | 1242556 |
| control of pyrimidine biosynthesis in human lymphocytes. inhibitory effect of guanine and guanosine on induction of enzymes for pyrimidine biosynthesis de novo in phytohemagglutinin-stimulated lymphocytes. | human peripheral lymphocytes were incubated with phaseolus vulgaris phytohemagglutinin. the induction of glutamine-utilizing carbamyl phosphate synthetase (ec 2.7.2.5) and aspartate transcarbamylase (ec 2.1.3.2) for pyrimidine biosynthesis de novo and the induction of uridine kinase were observed as described previously (ito, k., and uchino, h. (1971) j. biol. chem. 246, 4060-4065; ito, k., and uchino, h. (1973) j. biol. chem. 248, 389-392; lucas, z.j. (1967) science 156, 1237-1240). by the addi ... | 1976 | 1254576 |
| digestibility of protein and dry matter in caecectomised and intact rats. | the dry matter digestibility (dmd) of sifted granulated maize meal and the digestibility of the protein in yellow haricot beans were determined in intact and caecectomised rats. the experiments were done to ascertain whether microbial activity in the caecum has an effect on the digestibility. removal of the caecum resulted in a decrease of 9 percentage units in the dmd value but had no appreciable effect on the protein digestibity data. | 1975 | 1166366 |
| aminoacylation of phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial trnasmet and of escherichia coli trnasmet by homologous and heterologous enzymes. | met-trna synthetase from paseolus vulgaris cytoplasm could be separated from its chloroplastic or mitochondrial counterpart by deae-cellulose chromatography, but the met-trna synthetase from the two latter organelles could not be distinguished using deae-cellulose, hydroxyapatite or cm-sephadex chromatography. as revealed by reverse-phase chromatography, bean cytoplasm contains 2 trnasmet; only one is charged by chloroplast, mitochondrial or escherichia coli met-trna synthetase. mitochondria con ... | 1975 | 1101967 |
| aminoacylation of trna-leu species from escherichia coli and from the cytoplasm, chloroplasts and mitochondria of phaseolus vulgaris by homologous and heterologous enzymes. | leucyl-trna synthetase from phaseolus vulgaris chloroplasts could be separated from its cytoplasmic counterpart upon chromatography on hydroxyapatite, but the cytoplasmic and mitochondrial leucyl-trna synthetases could not be distinguished. the trnaleu species from the various plant cell compartments and from escherichia coli were aminoacylated using either homologous or heterologous enzymes; the levels of aminoacylation and the profiles of the leucyl-trnas upon reverse-phase chromatography were ... | 1975 | 1091292 |
| the binding of phytohemagglutinin m to rat spleen lymphocytes. quantitative studies. | phytohemagglutinin m (pham) has been purified from the commercial mixture of proteins produced by phaseolus vulgaris, using a sepharose-thyroglobulin column. the protein gave one band on gel electrophoresis and two bands on sds-gel electrophoresis (mol. wt. 33 700 and 32 100, respectively). molecular weight determination by ultracentrifugation gave a value of 61 200 +/- 700. the protein had a minimum sugar content of 16%. binding studies of pham to purified rat spleen lymphocytes have been perfo ... | 1976 | 953851 |
| aminoacylation of phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial trnaspro and trnaslys by homologous and heterologous enzymes. | the cytoplasmic prolyl-trna synthetase can be separated by hydroxyapatite chromatography, from the enzyme present in the chloroplasts and in the mitochondria (organellar enzyme). the cytoplasmic lysyl-trna synthetase can also be separated from the organellar enzyme. there are two trnaspro in the cytoplasm; they can be charged by the cytoplasmic enzyme, but not by the organellar enzyme or the escherichia coli enzyme. chloroplasts contain, in addition to the two cytoplasmic trnaspro, one chloropla ... | 1976 | 953001 |
| [effect of various heat treatments in the hemagglutinin content and in the protein quality of beans (phaseolus vulgaris)]. | the effect of pre-soaking raw seed beans upon detoxification and the biological quality of its protein were evaluated. in whole raw seed beans (phaseolus vulgaris) var. "tórtola", the net protein utilization (npu), true digestibility and hemagglutinin titer were determined after 60', 90' and 120' of heat treatment, with and without 14 hours of pre-soaking. it is concluded that soaking prior to cooking is not necessary to eliminate the toxicity of dry beans, but that it does contribute to the sof ... | 1978 | 753177 |
| methylation of yeast trnaphe by enzymes from cytoplasm, chloroplasts and mitochondria of phaseolus vulgaris. | pure yeast trnaphe was used as a substrate to compare the trna methylating activities in phaseolus vulgaris cytoplasm, chloroplasts and mitochondria, in the presence of s-adenosyl[me-3h]methionine. the resulting [me-3h]-trnaphe was then analyzed, using the techniques of nucleotide sequence determination. cytoplasmic and mitochondrial enzymes catalyze the methylation (into m5c) of c48 present in the extra-loop, while chloroplast enzyme preparations catalyze the modification (into m1a) of a14 pres ... | 1978 | 737182 |
| [effects of technological processing on digestive and metabolic utilization of amino acids from various protein foods]. | the use of vegetable proteins such as legumes or oilseeds proteins is often restricted by antinutritional or toxic factors. therefore, it is usefull, in order to extend their consumption, to isolate proteins from most of the other components of the seeds. unfortunately, the technological processes may have some deleterious effects on the nutritive value of the proteins. moreover, the proteins isolates and concentrates need further processing to be texturated in order to look like conventional fo ... | 1978 | 707919 |
| stimulation of de novo synthesis of l-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in colletotrichum lindemuthianum elicitor-treated cell suspension cultures of french bean (phaseolus vulgaris). | (1) the regulation of the accumulation of the isoflavonoid-derived phytoalexin phaseollin in cell suspension cultures of dwarf french bean (phaseolus vulgaris/ has been investigated. (2) an elicitor preparation from cell walls of colletotrichum lindemuthianum, the causal agent of anthracnose disease of french bean, caused a marked accumulation of phaseollin in the cultures. the elicitor induced phaseollin accumulation to a level of 60% that obtained with the artificial elicitor autoclaved ribonu ... | 1979 | 476149 |
| phaseolus vulgaris l. var. hur 15--a potential indigenous source for commercial pha preparation. | mitogenic potential of the partially purified lectin from p. vulgaris isolated by ammonium sulphate precipitation was assessed by lymphocyte transformation test (ltt) and was compared with three commercially available phytohemagglutinins (pha). the blast inducing capacity and mitotic index analysis revealed that this preparation has potential to be used in the indigenous commercial production of pha which is routinely used for human chromosomal studies from the peripheral blood culture at, at pr ... | 1992 | 1294485 |
| effect of gamma irradiation on nutritional value of dry field beans (phaseolus vulgaris) for chicks. | the effect of gamma irradiation (60co) of different varieties and breeding lines of dry field beans (phaseolus vulgaris) on chick growth was determined using a chick growth assay in which the diet contained approximately 50% beans. total protein (n x 6.25) in beans was not changed appreciably by irradiation (21 mrad) but protein solubility in water was decreased. irradiation increased in vitro enzymatic digestibility of bean protein by pepsin and by a mixture of trypsin, chymotrypsin and peptida ... | 1979 | 448472 |
| [the use of the mobile bag technique in swine. determination of the apparent crude protein digestibility and amino acid absorption of feed and rations]. | the mobile bag technique (mbt) was used in two ileorectostomized and two intact pigs with duodenal cannulae, in order to estimate the precaecal and total crude protein digestibility and amino acid (lysine, methionine, cystine, threonine, tryptophan) absorption of ten single feedstuffs (field bean, lupin, rapeseed meal, fish meal, barley, wheat) and three diets. for each feed 36 bags were applied per animal. after a peptic predigestion in vitro, application of the bags through the duodenal cannul ... | 1992 | 1296552 |
| effect of raw field bean (vicia faba) on amino-acid-degrading enzymes in rats and chicks. | the effects of vicia faba diet on urinary nitrogenous compounds and on enzyme activities of pathways directly associated with amino acid metabolism were studied in rats and chicks. the urea and creatinine excretion of rats fed on v. faba was approximately 90% more than that of control rats. the v.-faba-fed rats had increased activities of liver arginase (ec 3.5.3.1), argininosuccinate synthetase (ec 6.3.4.5) and alanine aminotransferase (ec 2.6.1.2). the chicks fed on v. faba also showed increas ... | 1979 | 424086 |
| a modification of the polyethylene glycol technique for the purification of small quantities of tobacco mosaic virus. | an adaptation of the polyethylene glycol method for the purification of tobacco mosaic virus (sunnhemp strain) from small (2 g) samples of french bean lamina is described. the virus can be precipitated from 1-2 ml of clarified sap by the addition of an equal volume of 8% polyethylene glycol 6000 (w/v) (peg), and pelleted by centrifugation for 10 min at 1,8000 x g. details are given of tests which establish that the purity and quality of the virus obtained by the small-scale method are as good as ... | 1979 | 393959 |
| metabolic factors and the utilization of phosphorus by plants. | the overall process of entry and transport of phosphate by plants has been separated into its component parts. rapid esterification is involved but a small proportion of the total transport may occur by a non-metabolic route. mannose alters the metabolism of phosphate in roots of cereals and thereby reduces the transport to the shoot by as much as 99% whereas dicotyledonous species are much less sensitive. the sequestration of phosphate as mannose 6-phosphate is reversible in some species depend ... | 1977 | 357118 |
| cooperativity of lectin binding to lymphocytes, and its relevance to mitogenic stimulation. | the relationship between the binding patterns of soybean agglutinin, peanut agglutinin (both in their native (unaggregated) form and in their polymerized form), and of phaseolus vulgaris leucoagglutinin, to neuraminidase-treated lymphocytes from different sources, and the mitogenic activity of these lectins, was studied. in all cases investigated, binding of a lectin to lymphocytes which resulted in stimulation was a positive cooperative process. our findings support the assumption that clusteri ... | 1978 | 305262 |
| inhibition of human neuroblastoma dna polymerase activities by plant lectins and toxins. | the effects of concanavalin a and ricin (rcaii, mr 65,000) on [3h]thymidine incorporation into human neuroblastoma imr-32 dna showed reduction of total dna synthesis to 50% and 70% of control, respectively. two dna polymerase (dna nucleotidyltransferase, ec 2.7.7.7.) activities (alpha and beta) involved in the biosynthesis in vitro of dna were separated by sucrose density gradient centrifugation from imr-32 cell homogenate. the dna polymerase alpha activity was also purified by selective precipi ... | 1979 | 287062 |
| imidoester inhibition of lymphocyte dna synthesis. | imidoesters amidinate free amino groups and produce inter- and intramolecular covalent bonds. to determine whether imidoesters influenced lymphocyte transformation, human peripheral blood or calf lymph node lymphocytes were cultured with dimethyladipimate (dma), a bifunctional (cross-linking) imidoester, or methyl acetimidate (mac), a monofunctional (noncross-linking) imidoester. both dma and mac decreased the rate of endogenous dna synthesis in a dose-dependent fashion. in further work, lymphoc ... | 1979 | 216487 |
| [physical, technological, and protein characteristics of wild and cultivated beans (phaseolus vulgaris l.)]. | the main purpose of this work was to observe and compare some physical, technological and proteinic characteristics of four bean seed cultivars developed in mexico, eight wild bean seed accessions collected in mexico and four wild bean seed accessions collected in south america. all of them belong to the species phaseolus vulgaris l. in the south american materials the seed size was very similar (mean = 10.4 g/100 seeds). nevertheless the water absorption was variable 36-64%, the percentages of ... | 1992 | 1341861 |
| spleen and thymus histology and proliferative response of splenic cells in rats fed raw and cooked phaseolus vulgaris beans. | histological studies of the spleen and thymus of rats fed raw black beans (phaseolus vulgaris) show an atrophy of both lymphoid organs. decrease in relative thymus weight was most marked. all histological organization of this organ appeared altered. an evident decrease in cell number was also observed in both organs. proliferative response of splenic cells stimulated in vitro with concanavalin a was increased as compared to that from animals fed the control diet. it is likely that histological c ... | 1992 | 1342176 |
| type i collagen synthesis by rats fed beans (phaseolus vulgaris, l.) as the protein source. | type i collagen synthesis was studied in 12 female wistar rats weighing 60 +/- 5 g at the beginning of the experiment. the animals were fasted for 24 h and then injected ip with 10 microci uniformly labeled [14c]-glycine. two hours later, groups of 4 animals each were fed balanced diets (10.7 +/- 0.4% protein) containing raw beans (phaseolus vulgaris, l.), cooked beans or casein (control) as the single protein source, ad libitum. the animals were killed after 4 days and collagen was extracted fr ... | 1992 | 1342225 |
| projections from rabbit caudal medulla to c1 and a5 sympathetic premotor neurons, demonstrated with phaseolus leucoagglutinin and herpes simplex virus. | we combined phaseolus vulgaris leucoagglutinin anterograde tracing and herpes simplex virus transneuronal retrograde tracing to determine whether neurons in the vasodepressor region of the rabbit caudal ventrolateral medulla project to brainstem neurons containing the virus after its transneuronal transport from the adrenal medulla. five days after adrenal injection of virus, 764 +/- 159 virus-positive neurons were found bilaterally in the brainstem: 61% in the c1 sympathoexcitatory region of th ... | 1992 | 1349616 |
| enhanced dna synthesis in isolated calf lymph node lymphocyte cultures. | calf sub-mandibular lymph node lymphocytes and human peripheral blood lymphocytes were oxidized by naio4. in liquid cell cultures, both types of cells were transformed with enhanced dna synthesis. in mixed lymphocyte cultures, untreated human lymphocytes were transformed by irradiated-oxidized human cells. however, untreated calf lymphocytes were not transformed by irradiated-oxidized calf cells. either human or calf lymphocytes were isolated from cell-cell interaction in agarose on slides. afte ... | 1977 | 139220 |