| rhodamine immunohistofluorescence applied to plant tissue. | tissue slices from the roots and seeds of sanifoin (onobrychis viciifolia, scop.) exhibit bright autofluorescence when illuminated with blue (495 nm) light. this autofluorescence is indistinguishable from the fluorescence emission of fluorescein, the commonly used fluorochrome in immunohistochemical staining procedures. rhodamine isothiocyanate, when coupled to immunoglobulin, and excited with green light at 546 nm, exhibits a reddish-orange fluorescence with an emission maximum at 590 nm. plant ... | 1978 | 353187 |
| isolation and properties of a lectin from sainfoin (onobrychis viciifolia, scop.). | a glycoprotein capable of binding simple carbohydrates and causing hemagglutination has been isolated from seeds of the legume plant sainfoin (onobrychis viciifolia, scop. var eski). the phytolectin was prepared by affinity chromatography of ph 7.0 sodium phosphate extracts on columns of sepharose-4b containing covalently attached d-mannose. molecular weight determinations showed the lectin to be a dimer consisting of 26 000 dalton, non-covalently associated monomers. amino acid analyses indicat ... | 1979 | 546436 |
| the glycosyl moiety of lectin from sainfoin (onobrychis viciifolia, scop.). | a lectin isolated from the seeds of sainfoin (onobrychis viciifolia, scop. var eski) has been shown to be a glycoprotein containing 2.6% (w/w) neutral carbohydrate and 1.6% (w/w) glucosamine (hapner, k.d. and robbins, j.e. (1979) biochim. biophys. acta 580, 186--197) a homogeneous glycopeptide accounting for 70% of the original glycoprotein carbohydrate was isolated from pronase digests of the lectin by gel filtration chromatography. gas-liquid chromatographic and amino acid analyses showed the ... | 1979 | 546437 |
| the effect of the condensed tannins of sainfoin (onobrychis viciaefolia) on the release of soluble leaf protein into the food bolus of cattle [proceedings]. | | 1976 | 972907 |
| technical note: utilization of sainfoin by grazing steers and a method for predicting daily gain from small-plot grazing data. | sainfoin (onobrychis viciaefolia scop.) is adapted to the calcareous soils of the southern great plains and can provide early season forage that does not induce bloating; however, little is known about performance by ruminants grazing sainfoin. our objective was to determine the effect of plant growth stage and grazing pressures on potential animal production from sainfoin as predicted from energy intake as a multiple of maintenance. nitrogen-fertilized (100 kg of n/ha) renumex sainfoin was grow ... | 1992 | 1644700 |
| the dinitrophenyl group as a selective label in high-performance liquid chromatography of peptides. | 1-fluoro-2,4-dinitrobenzene can be used to selectively label histidine, tyrosine, and cysteine residues in maleylated proteins. the usefulness of the resulting chromophores for peptide mapping by high-performance liquid chromatography was demonstrated with the lectin from sainfoin (onobrychis viciifolia). the 2,4-dinitrophenyl (dnp) label also can be used in a hydrophobic modulation approach as the mobility of a labeled model peptide changes considerably when its dnp group is removed by thiolysi ... | 1987 | 3605591 |
| [comparative study of the pollen of diploid and autotetraploid forms of onobrychis l. species]. | | 1974 | 4467363 |
| the effect of leuco-anthocyanins in sainfoin (onobrychis viciifolia scop.) on the availability of protein to sheep and upon the determination of the acid detergent fibre and lignin fractions. | | 1971 | 5090461 |
| the digestion of dried lucerne (medicago sativa l.) and dried sainfoin (onobrychis viciifolia scop.) by sheep. | | 1971 | 5090462 |
| [flavonoids of leguminous plants: research on flowers of cercis siliquastum l, onobrychis viciaefolia scop. (=o. sativa lam.) and melilotus alba desr]. | | 1969 | 5368580 |
| [diapause of eggs from the pea aphid (acyrthosiphon onobrychis boyer, homoptera, aphididae)]. | | 1970 | 5529642 |
| [effect of photoperiodicity on the fertility, longevity and formation of alate and apterous forms of the pea louse (acyrthosiphon onobrychis boyer)]. | | 1968 | 5666259 |
| lectin from sainfoin (onobrychis viciifolia scop.). complete amino acid sequence. | the complete amino acid sequence of a lectin from sainfoin ( onobrychis viciifolia scop . var. eski ) has been determined by sequential edman analyses of the intact protein and peptides derived from digests with trypsin and thermolysin. peptides were purified by ph fractionation, by gel filtration, and by cation-exchange and reverse-phase high-performance liquid chromatography. seven segments of continuous sequence, accounting for the entire protein, were aligned through sequence comparison with ... | 1984 | 6722125 |
| structural comparison of the lectin from sainfoin (onobrychis viciifolia) with concanavalin a and other d-mannose specific lectins. | the d-mannose specific lectin from sainfoin was prepared by affinity chromatography on sephadex g-75, and its circular dichroism (cd), metal content, antigenic character, and n-terminal amino acid sequence were compared with those of four lectins from vicieae plants and concanavalin a. the sainfoin lectin was only slightly more closely related to these other d-mannose specific lectins, than to lectins of leguminous plants in general. the cd and antigenic experiments also confirmed the close rela ... | 1982 | 7172086 |
| in vitro digestion of bloat-safe and bloat-causing legumes by rumen microorganisms: gas and foam production. | leaves of three bloat-safe legumes -- birdsfoot trefoil (lotus corniculatus l.), sainfoin (onobrychis viciaefolia scop.), and cicer milkvetch (astralagus cicer l.) -- and of three bloat-causing legumes -- alfalfa (medicago sativa l.), red clover (trifolium pratense l.), and white clover (trifolium repens l.) -- were incubated with strained rumen fluid or with mixed rumen fluid and solids. gas released was measured during the early period (0 to 22 h) of this in vitro digestion. gas volume was gre ... | 1980 | 7419778 |
| pasture management strategies for reducing the risk of legume bloat in cattle. | results from two decades (1973-1993) of bloat research at kamloops are reviewed. the trials were conducted with groups of ruminally fistulated cattle either grazing or fed daily fresh-cut alfalfa (medicago sativa) herbage. studies were conducted during the growing season (may to september) and in the fall (october and november). the alfalfa was usually in the vegetative to early bloom stages of growth. visual assessments of bloat severity on a scale of 1 to 5 were made .5 to 2 h after feeding st ... | 1995 | 7665381 |
| proanthocyanidins inhibit hydrolysis of leaf proteins by rumen microflora in vitro. | proanthocyanidins (condensed tannins; pa) purified from the leaves of forage legumes trifolium arvense, lotus pedunculatus, lotus corniculatus, dorycnium rectum, coronilla varia, onobrychis viciifolia, or hedysarum coronarium, were added to soluble lucerne (medicago sativa) leaf protein and incubated with strained rumen fluid in vitro. fractions were collected and frozen immediately. denatured proteins were fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page), sta ... | 1994 | 8031741 |
| synthesis of 3,4-cis-[3h]leucocyanidin and enzymatic reduction to catechin. | a novel method is presented for the synthesis and purification of (+)-2,3-trans-3,4-cis-[4-3h]leucocyanidin. soluble enzyme extracts from developing barley grains and leaves of the forage legume onobrychis viciifolia (sainfoin) catalyzed the nad(p)h-dependent reduction of (+)-2,3-trans-3,4-cis-[4-3h]leucocyanidin to (+)-[4-3h]catechin. nadph was the preferred substrate. with extracts of barley the rate of reaction with 1 mm nadh was 20% of the rate found with nadph. with extracts from both tissu ... | 1993 | 8470799 |
| sequence and mutational analysis of the common nodbcij region of rhizobium sp. (oxytropis arctobia) strain n33, a nitrogen-fixing microsymbiont of both arctic and temperate legumes. | by heterologous hybridization, we have identified the common nodulation genes nodbcij of rhizobium sp. strain n33 within a 8.2-kb psti fragment. the nodbcij genes are located within a 4,620-bp region which also included a consensus nod box promoter. the four open reading frames coding for the nodbcij genes contain 657, 1,353, 915, and 789 nucleotides, respectively. we found that the noda gene was not adjacent to the nodb gene, unlike the situation in many rhizobia. the dna of the nodbcij genes o ... | 1996 | 8755627 |
| characterization and mutational analysis of nodhpq genes of rhizobium sp. strain n33. | we have shown, by sequencing the nodulation gene region of rhizobium sp. strain n33 previously isolated from the canadian high arctic, that the nodhpq genes are located in a 4.8-kb region downstream of nodbcij. the open reading frames of nodhpq are 747, 906, and 1941 nucleotides long, respectively. the strain n33 genome contains one copy of nodh and two copies of nodpq that are homologous to those genes in rhizobium meliloti. tn5 insertions in the nodhpq genes of strain n33 did not affect the fo ... | 1996 | 8870271 |
| sequence and mutational analysis of the 6.7-kb region containing nodafeg genes of rhizobium sp. strain n33: evidence of dna rearrangements. | a 6.7-kb region upstream of nodbc genes in rhizobium sp. strain n33 was shown to contain the nodafeg genes and an open reading frame designated orfz. the open reading frames for these genes contain 591, 282, 1209, 738, and 1,338 nucleotides respectively. homologues of these genes were found in other rhizobia with the exception of orfz, for which there was no counterpart found in the genbank/embl database. tn5 mutagenesis in nodeg and in the intergenic nodg-b region has shown a nod+ phenotype on ... | 1997 | 9100384 |
| genetic diversity of rhizobial symbionts isolated from legume species within the genera astragalus, oxytropis, and onobrychis. | the genetic diversity of 44 rhizobial isolates from astragalus, oxytropis, and onobrychis spp. originating from different geographic locations was evaluated by mapped restriction site polymorphism (mrsp) analysis of 16s rrna genes and by pcr dna fingerprinting with repetitive sequences (rep-pcr). a comparison of tree topologies of reference strains constructed with data obtained by mrsp and by 16s rrna gene sequence analyses showed that the topologies were in good agreement, indicating that the ... | 1997 | 9406393 |
| variation of selenium content in growing wild plants during vegetative period. | there is evidence that selenium (se) is essential for humans, animals, and some species of microorganisms. this study examines the dynamics of the change of se accumulation in some growing wild plants: astragalus onobrychis var. chlorocarpus (gris.) stoj. et stef. (leguminosae), oxytropis pilosa (l.) dc., salvia officinalis, l., and simphytum ottomanum l. the influences of some ecological factors (se content in soil, temperature, and rainfall) have also been surveyed. the se content of plants fr ... | 1998 | 9726793 |
| comparison of kinetic properties of amine oxidases from sainfoin and lentil and immunochemical characterization of copper/quinoprotein amine oxidases. | kinetic properties of novel amine oxidase isolated from sainfoin (onobrychis viciifolia) were compared to those of typical plant amine oxidase (ec 1.4.3.6) from lentil (lens culinaris). the amine oxidase from sainfoin was active toward substrates, such as 1,5-diaminopentane (cadaverine) with k(m) of 0.09 mm and 1,4-diaminobutane (putrescine) with k(m) of 0.24 mm. the maximum rate of oxidation for cadaverine at saturating concentration was 2.7 fold higher than that of putrescine. the amine oxidas ... | 1999 | 10092944 |
| [dna homology in various strains of nitrogen-fixing bacteria]. | melting temperature and gc content were evaluated for dna of some nitrogen-fixing bacteria of rhizobium leguminosarum and onobrychis spp. (adans). the degree of homology between strains of the same species was determined. a combination of thermal denaturing and molecular hybridization can serve as a rapid test for evaluating the genome homology of the organisms compared. | 2000 | 10702988 |
| the effect of condensed tannins from seven herbages on trichostrongylus colubriformis larval migration in vitro. | the effects of condensed tannins (ct) extracted from seven forages on the motility of the economically important nematode, trichostrongylus colubriformis (giles, 1892), were evaluated by using a larval migration inhibition (lmi) assay. the assay involved incubation of third stage (l3) exsheathed t. colubriformis larvae with ct extracted from lotus pedunculatus, lotus corniculatus, sulla (hedysarum coronarium), sainfoin (onobrychis viciifolia), dorycnium rectum, dorycnium pentaphyllum and dock (r ... | 2000 | 10833014 |
| polyphenols, condensed tannins, and other natural products in onobrychis viciifolia (sainfoin). | an acetone/water extract of the fodder legume onobrychis viciifolia afforded arbutin, kaempferol, quercetin, rutin, afzelin, the branched quercetin-3-(2(g)-rhamnosylrutinoside), the amino acid l-tryptophan, the inositol (+)-pinitol, and relatively high concentrations of sucrose (ca. 35% of extractable material). acid-catalyzed cleavage of the condensed tannins with phloroglucinol afforded catechin, epicatechin and gallocatechin as the terminal and extender units, but epigallocatechin was only pr ... | 2000 | 10956131 |
| phenolic glycosides of forage legume onobrychis viciifolia. | a chemical examination of the extractives of the leaves of sainfoin was undertaken as part of a programme directed at understanding the factors which may contribute to its nutritive value as animal feed. among the low molecular weight phenolic compounds characterized were seven cinnamic acid derivatives and nine flavonoid glycosides all of which were identified by nmr spectroscopy. included among these compounds were two new natural hydroxycinnamic esters namely methyl 6-o-p-trans-coumaroyl-beta ... | 2000 | 11021646 |
| three new arylobenzofurans from onobrychis ebenoides and evaluation of their binding affinity for the estrogen receptor. | three new 2-phenyl-benzofurans, ebenfuran i, ebenfuran ii, and ebenfuran iii, were isolated from onobrychis ebenoides. their structures were elucidated on the basis of chemical and spectral data as 2-(2,4-dihydroxyphenyl)-5-hydroxy-6-methoxy-benzofuran (1), 2-(2,4-dihydroxyphenyl)-3-formyl-4-hydroxy-6-methoxy-benzofuran (2), and 2-(2, 4-dihydroxyphenyl)-3-formyl-4-hydroxy-6-methoxy-5-(3-methyl-buten- 2-y l)-benzofuran (3). the affinity of these compounds for the estrogen receptor was studied usi ... | 2000 | 11141112 |
| unusual methyl-branched alpha,beta-unsaturated acyl chain substitutions in the nod factors of an arctic rhizobium, mesorhizobium sp. strain n33 (oxytropis arctobia). | mesorhizobium sp. strain n33 (oxytropis arctobia), a rhizobial strain isolated in arctic canada, is able to fix nitrogen at very low temperatures in association with a few arctic legume species belonging to the genera astragalus, onobrychis, and oxytropis. using mass spectrometry and nuclear magnetic resonance spectroscopy, we have determined the structure of n33 nod factors, which are major determinants of nodulation. they are pentameric lipochito-oligosaccharides 6-o sulfated at the reducing e ... | 2001 | 11371536 |
| [transformation of sainfoin by agrobacterium rhizogenes lba9402 bin19 and regeneration of transgenic plants]. | hypocotyl segments of onobrychis viciaefolia were transformed by agrobacterium rhizogenes lba9402 which harboured pbin19 and pri1855. seedling age and preculture time of hypocotyl segments influenced the transformation frequency. paper electrophoresis revealed that 70% of single hairy root cultures could synthesize agropine. calli were induced from hairy root segments on ms medium containing 0-9.05 mumol/l 2,4-d and 0-2.22 mumol/l 6-ba at first, then they were transferred onto ms0 medium without ... | 2000 | 12548853 |
| [role of bacteria of tubercles in adaptation of onobrychis in dry desert regions]. | | 1952 | 12992617 |
| [embryology of the papilionacease; development of the embryo in onobrychis sativa lam..]. | | 1953 | 13082810 |
| [physiological peculiarities of nutrition of onobrychis sativa]. | | 1952 | 14916793 |
| 1,5-diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases. | 1,5-diamino-2-pentyne (dapy) was found to be a weak substrate of grass pea (lathyrus sativus, gpao) and sainfoin (onobrychis viciifolia, ovao) amine oxidases. prolonged incubations, however, resulted in irreversible inhibition of both enzymes. for gpao and ovao, rates of inactivation of 0.1-0.3 min(-1) were determined, the apparent ki values (half-maximal inactivation) were of the order of 10(-5) m. dapy was found to be a mechanism-based inhibitor of the enzymes because the substrate cadaverine ... | 2004 | 15606757 |
| characteristics of the rhizobia associated with dalea spp. in the ordway, kellogg-weaver dunes, and hayden prairies. | habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. in this paper, we consider the diversity of the rhizobia associated with the indigenous legume dalea purpurea in 3 residual prairie areas in minnesota and iowa. using dalea purpurea as a trap host, 218 rhizobia were recovered from these soils then characterized using boxa1r pcr. three major and 13 minor groups were distinguished b ... | 2005 | 15782230 |
| mechanism-based inactivators of plant copper/quinone containing amine oxidases. | copper/quinone amine oxidases contain cu(ii) and the quinone of 2,4,5-trihydroxyphenylalanine (topaquinone; tpq) as cofactors. tpq is derived by post-translational modification of a conserved tyrosine residue in the protein chain. major advances have been made during the last decade toward understanding the structure/function relationships of the active site in cu/tpq amine oxidases using specific inhibitors. mechanism-based inactivators are substrate analogues that bind to the active site of an ... | 2005 | 16054177 |
| characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion. | to identify simple screening tools for selecting condensed tannin (ct)-containing forages as candidate sources for further study, ct were isolated from nine legumes, and their molecular weights (mw), chromophore production, capacity to precipitate bovine serum albumin (bsa) and fraction 1 protein (rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. sources were as follows: leaves of sericea lespedeza (lespedeza cuneata dum.-cours.), crown vetch (coronilla vari ... | 2005 | 16132212 |
| effect of bioactive compounds from sainfoin ( onobrychis viciifolia scop.) on the in vitro larval migration of haemonchus contortus: role of tannins and flavonol glycosides. | anthelmintic bioactivity against gastrointestinal nematodes has been associated with leguminous forages supporting the hypothesis of a role of condensed tannins. however, the possibility that other compounds might also been involved has received less consideration. using bio-guided fractionation, the current study aimed at characterizing the biochemical nature of the active compounds present in sainfoin (onobrychis viciifolia ), previously identified as an anthelmintic leguminous forage. the eff ... | 2005 | 16174418 |
| purification and thermal dependence of glutathione reductase from two forage legume species. | alfalfa (medicago sativa l.) and sainfoin (onobrychis viciifolia scop.) are forage legumes that differ in their responses to high and low temperature stresses. thermal limitations on the function of glutathione reductase (ec 1.6.4.2) could adversely affect the ability of the plant to cope with adverse temperatures. our objectives were to (a) purify glutathione reductase from ;cimarron' alfalfa and ;pi 212241' sainfoin and (b) investigate the intraspecies variation in the thermal dependency of gl ... | 1990 | 16667283 |
| endophytic occupation of root nodules and roots of melilotus dentatus by agrobacterium tumefaciens. | agrobacterium strains have been frequently isolated from the root nodules of different legumes. various possible mechanisms have been proposed to explain the existence of these bacteria in nodules, but there is no sufficient experimental evidence to support the estimations. in this work, we proved that the agrobacterium strain ccbau 81181, which was originally isolated from the root nodules of onobrychis viciaefolia, and a symbiotic strain of sinorhizobium meliloti ccbau 10062 could coinhabit th ... | 2006 | 16897296 |
| effect of sainfoin (onobrychis viciifolia) silage and hay on established populations of haemonchus contortus and cooperia curticei in lambs. | the objective of the study was to examine the effect of dried and ensiled sainfoin (onobrychis viciifolia) on established populations of haemonchus contortus (abomasum) and cooperia curticei (small intestine) in lambs under controlled conditions. twenty-four parasite naïve lambs were inoculated with a single dose of infective larvae of these parasites 28 days prior to the start of the feeding experiment. twenty-four days post-infection, 4 days prior to the start of the feeding experiment, animal ... | 2006 | 16934938 |
| biomass allocation is an important determinant of the tannin concentration in growing plants. | condensed tannins (cts) in the diet affect consumers in a concentration-dependent manner. because of their importance in plant defence against herbivores and pathogens as well as their potential application against gastrointestinal parasites of ruminants in agronomy, an understanding of the seasonal dynamics of ct concentrations during plant growth is essential. | 2007 | 17210606 |
| flavonoids from astragalus onobrychis. | | 1995 | 17238067 |
| individual administration of three tanniferous forage plants to lambs artificially infected with haemonchus contortus and cooperia curticei. | we investigated direct anthelmintic effects associated with the feeding of fresh tanniferous forages against established populations of haemonchus contortus and cooperia curticei in lambs. twenty-four parasite naive lambs were inoculated with a single dose of infective larvae of these two parasites 27 days prior to the start of the feeding experiment. lambs were individually fed with either chicory (cichorium intybus), birdsfoot trefoil (lotus corniculatus), sainfoin (onobrychis viciifolia) or a ... | 2007 | 17336459 |
| influence of harvest time on fuel characteristics of five potential energy crops in northern china. | five potential energy crops in northern china were examined for fuel characteristics over different harvest times to test whether or not a delayed harvest improves fuel quality in a semiarid area in china as is the case in northern europe and north america. the five crops include indigo bush (amorpha fruticosa), sand willow (salix cheilophila), switch grass (panicum virgatum), reed canary grass (phalaris arundinacea), and sainfoin (onobrychis viciifolia). these crops are considered as fuels for ... | 2008 | 17382539 |
| characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china. | qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ... | 2007 | 17541555 |
| palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (onobrychis viciifolia) birdsfoot trefoil (lotus corniculatus), and chicory (cichorium intybus). | three temperate forages, sainfoin, birdsfoot trefoil, and chicory, characterized by elevated contents of plant secondary compounds, were compared to a ryegrass-clover mixture (control) in dried (experiment 1) and ensiled form (experiment 2) in their palatability and nutritional value. palatability was measured in adult wethers (n = 6) allowed to choose between the familiar control forage and one of the three test plants. palatability index was calculated from differences in intake of control and ... | 2007 | 18069619 |
| effects of sainfoin (onobrychis viciifolia) extract and monomers of condensed tannins on the association of abomasal nematode larvae with fundic explants. | tannin-rich forages offer an alternative to anthelmintic chemicals to control gastrointestinal nematodes. however, the mode of action of such bioactive plants still needs to be assessed. previous studies have shown that extracts of tannin-rich plants interfere with the first phase of host invasion, i.e., the exsheathment of infective larvae (l3s). in the current study, we examined the hypothesis that exposure to tannins could also affect the second phase of larval establishment, i.e., the tissue ... | 2008 | 18093592 |
| nitrogen and mineral balance of lambs artificially infected with haemonchus contortus and fed tanniferous sainfoin (onobrychis viciifolia). | tanniferous temperate legumes are assumed to possess anthelmintic properties, but it is unclear whether this is the direct result of condensed tannins (ct) or is mediated indirectly via an improved metabolic protein supply. a metabolism experiment was conducted to differentiate between these factors by feeding the ct plant sainfoin (19.7% cp in dm) to lambs infected with the abomasal blood-sucking nematode hemonchus contortus. a total of 18 infected lambs were fed sainfoin either untreated or tr ... | 2008 | 18375660 |
| interference of condensed tannin in lignin analyses of dry bean and forage crops. | legumes with high concentrations of condensed tannin (pinto bean [phaseolus vulgaris l.], sainfoin [onobrychis viciifolia scop.], and big trefoil [lotus uliginosus hoff.]), were compared to a selection of forages, with low or zero condensed tannin (smooth bromegrass [ bromus inermis leyss], lotus japonicus [regel] k. larsen, and alfalfa [medicago sativa l.]), using four methods to estimate fiber or lignin. protocols were validated by using semipurified condensed tannin polymers in adulteration a ... | 2008 | 18841900 |
| ebenfurans iv-viii from onobrychis ebenoides: evidence that c-prenylation is the key determinant of the cytotoxicity of 3-formyl-2-arylbenzofurans. | phytochemical investigation of a methanol extract of onobrychis ebenoides yielded five new 3-formyl-2-arylbenzofurans, namely, ebenfurans iv-viii (1-5), together with the known compounds ebenfurans i, ii (6), and iii (7). only 1 and 7 exhibited growth inhibitory activity against mcf-7 and ishikawa cells, suggesting that the prenyl moiety at position c-5 is the key determinant of the cytotoxic activity of this group of compounds. | 2008 | 18986200 |
| potential to reduce escherichia coli shedding in cattle feces by using sainfoin (onobrychis viciifolia) forage, tested in vitro and in vivo. | there is a growing concern about the presence of pathogens in cattle manure and its implications on human and environmental health. the phytochemical-rich forage sainfoin (onobrychis viciifolia) and purified phenolics (trans-cinnamic acid, p-coumaric acid, and ferulic acid) were evaluated for their ability to reduce the viability of pathogenic escherichia coli strains, including e. coli o157:h7. mics were determined using purified phenolics and acetone extracts of sainfoin and alfalfa (medicago ... | 2009 | 19098216 |
| evidence for the inhibition of the terminal step of ruminal alpha-linolenic acid biohydrogenation by condensed tannins. | effects of condensed tannins (ct), either via extract or plant-bound, and saponin extract on ruminal biohydrogenation of alpha-linolenic acid (ala) were investigated in vitro. grass-clover hay served as basal diet (control). the control hay was supplemented with extracts contributing either ct from acacia mearnsii [7.9% of dietary dry matter (dm)] or saponins from yucca schidigera (1.1% of dm). the fourth treatment consisted of dried sainfoin (onobrychis viciifolia), a ct-containing forage legum ... | 2009 | 19109277 |
| phytoremediation of oil-sludge-contaminated soil. | the aim of this research was to select plant species that could be effective in the phytoremediation ofa former oil-sludge pit. seven crop plants (triticum aestivum l., secale cereale l., avena sativa l., hordeum vulgare, sorghum bicolor l moench, panicum miliaceum l, and zea mays l.),five wild grasses (lolium perenne l., bromopsis inermis, agropyron cristatum l., agropyrum tenerum l., and festuca pratensis huds.), and three legumes (medicago sativa l., trifolium pratense l., and onobrychis anta ... | 2008 | 19260228 |
| evaluation of cultivars, experimental lines and plant introduction collection of sainfoin for resistance to meloidogyne hapla chitwood. | stands of several cultivars and experimental lines of sainfoin (onobrychis viciifolia) were severely reduced (92% average loss) in a field naturally infested with meloidogyne hapla. stands of two alfalfa cultivars included in the test were unaffected. in studies conducted in the greenhouse with plants inoculated at the time of seeding, average mortality was 55% for sainfoin entries and 7% for ladak alfalfa. little mortality occurred when plants were inoculated after establishment. three months a ... | 1987 | 19290103 |
| identification and quantification of phenolic compounds from the forage legume sainfoin ( onobrychis viciifolia ). | phenolic compounds of sainfoin ( onobrychis viciifolia ) variety cotswold common are assumed to contribute to its nutritive value and bioactive properties. a purified acetone/water extract was separated by sephadex lh-20 gel chromatography. sixty-three phenolic and other aromatic compounds were identified by means of chemical, chromatographic, and spectroscopic methods. reverse phase hplc with diode array and chemical reaction detection was used to investigate the phenolic composition of differe ... | 2009 | 19456170 |
| anthelmintic activity of some mediterranean browse plants against parasitic nematodes. | the anthelmintic properties of tannin-rich plants are being explored as an alternative to chemical drugs. most data have been acquired on legume forages, but only few on browse plants. the present study aimed to (i) screen the in vitro effects of extracts from 7 mediterranean plants on haemonchus contortus, (ii) verify the role of tannins using an inhibitor, polyvinyl polypyrrolidone (pvpp) and (iii) verify the in vivo effects of extracts from 4 plants. significant inhibition was shown in vitro ... | 2010 | 19961649 |
| effect of feeding bioactive forages on infection and subsequent development of haemonchus contortus in lamb faeces. | contrasting dried herbage diets were offered to lambs to evaluate their effects on haemonchus contortus infection and on subsequent development of larvae in faeces. artificially infected lambs (n=24, 4 months old) were allocated to one of four treatment groups (n=6) and fed on hay of lucerne (medicago sativa; lu), sulla (hedysarum coronarium; su), sainfoin (onobrychis viciifolia; sa) or wormwood (artemisia absinthium; wo) included as 20% in ground lucerne pellets. while voluntary intake was simi ... | 2010 | 20554118 |
| legume species differ in the responses of their functional traits to plant diversity. | plants can respond to environmental impacts by variation in functional traits, thereby increasing their performance relative to neighbors. we hypothesized that trait adjustment should also occur in response to influences of the biotic environment, in particular different plant diversity of the community. we used 12 legume species as a model and assessed their variation in morphological, physiological, life-history and performance traits in experimental grasslands of different plant species (1, 2 ... | 2011 | 20680645 |
| optimization of a high-performance liquid chromatography method for the analysis of complex polyphenol mixtures and application for sainfoin extracts (onobrychis viciifolia). | a pentafluorophenylpropyl (pfp) stationary phase was tested for the simultaneous determination of several classes of phenolic compounds. the chromatographic results were compared with those obtained by using a bifunctional phase constituted of octadecyl and phenylpropyl bonded silica and three conventional c18 columns. the elution gradient was optimized with 5% formic acid and sodium acetate in combination with acetic acid as additives and methanol as solvents. for these evaluations, a complex p ... | 2010 | 20817190 |
| effect of environmental factors and influence of rumen and hindgut biogeography on bacterial communities in steers. | feces from cattle production are considered important sources of bacterial contamination of food and the environment. little is known about the combined effects of arctic temperatures and fodder tannins on rumen and hindgut bacterial populations. individual rumen liquor and rectal fecal samples from donor steers fed either alfalfa silage or sainfoin (onobrychis viciifolia scop.) silage and water ad libitum were collected weekly on the first three sampling days and fortnightly afterwards. the dai ... | 2010 | 21075877 |
| in situ analysis and structural elucidation of sainfoin (onobrychis viciifolia) tannins for high-throughput germplasm screening. | a rapid thiolytic degradation and cleanup procedure was developed for analyzing tannins directly in chlorophyll-containing sainfoin ( onobrychis viciifolia ) plants. the technique proved suitable for complex tannin mixtures containing catechin, epicatechin, gallocatechin, and epigallocatechin flavan-3-ol units. the reaction time was standardized at 60 min to minimize the loss of structural information as a result of epimerization and degradation of terminal flavan-3-ol units. the results were ev ... | 2010 | 21175139 |
| do sheep (ovis aries) categorize plant species according to botanical family? | the ability of grazing herbivores to assign food types to categories by relying on certain relevant criteria could considerably reduce cognitive demand and increase their foraging efficiency when selecting among many different plant items. grasses and legumes differ functionally in vegetation communities as well as in nutritive value. we aimed to determine whether sheep can generalize an aversion they learnt for a grass or a legume species to another species of the same functional type and conse ... | 2011 | 21203791 |
| acylated flavonol glycosides from the forage legume, onobrychis viciifolia (sainfoin). | ten acylated flavonol glycosides were isolated from aqueous acetone extracts of the aerial parts of the forage legume, onobrychis viciifolia, and their structures determined using spectroscopic methods. among these were eight previously unreported examples which comprised either feruloylated or sinapoylated derivatives of 3-o-di- and 3-o-triglycosides of kaempferol (3,5,7,4'-tetrahydroxyflavone) or quercetin (3,5,7,3',4'-pentahydroxyflavone). the diglycosides were acylated at the primary glc res ... | 2011 | 21292287 |
| deciphering the complexity of sainfoin ( onobrychis viciifolia ) proanthocyanidins by maldi-tof mass spectrometry with a judicious choice of isotope patterns and matrixes. | use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin ( onobrychis viciifolia ) by maldi-tof mass spectrometry. proanthocyanidins contained predominantly b-type homopolymers and heteropolymers up to 12-mers (3400 da). use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of a-type glycosylated dimers. in addition, we report here how a comparison of the isotopic adduct patterns, which resulted from li a ... | 2011 | 21488615 |
| ultrastructural changes in the third-stage, infective larvae of ruminant nematodes treated with sainfoin (onobrychis viciifolia) extract. | plants rich in condensed tannins are an alternative to chemical anthelmintics to control gastrointestinal nematodes (gins) in ruminants. previous functional studies have shown that sainfoin extracts affect the two forms of infective larvae (l3), ensheathed and exsheathed. however, the mechanisms of action remain unknown. the aim of this study was thus to compare ultrastructural changes in ensheathed and exsheathed l3 of two gin species after in vitro contact with sainfoin extracts using transmis ... | 2011 | 21787880 |
| condensed tannins act against cattle nematodes. | the use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (gin) in ruminants. direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat gin. these anthelmintic properties are mainly associated with condensed tannins. in the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine gin. effects of onobrychis viciifolia, lotus pedunculatus and lotus cornic ... | 2011 | 21726942 |
| Fermentation kinetics of sainfoin hay with and without PEG. | The in vitro gas production technique was applied to evaluate the biological activity of tannins in sainfoin (Onobrychis viciifolia Scop.) hay cut at four progressive phenological stages. The buffalo (Bubalus bubalis) rumen fluid was used as inoculum, polyethylene glycol (PEG) was added as specific binding agent and an alfalfa hay (AH) was used as control. All samples were analysed for chemical composition and condensed tannin (CTs). According to the phenological stage of sainfoin, crude protein ... | 2011 | 22168179 |
| Simple solution for a complex problem: proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography. | This study was undertaken to explore gel permeation chromatography (GPC) for estimating molecular weights of proanthocyanidin fractions isolated from sainfoin (Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethylene glycol and polymethyl methacrylate standards were not suitable for estimating the molecular weights of underivatized proanthocyanidins. Therefore, a novel HPLC-GPC method was developed based on two ... | 2011 | 21930278 |
| a simple method for primary screening of antibacterial peptides in plant seeds. | regarding the importance of finding new antibacterial drugs, screening of plants as a promising resource are now conducted worldwide. in this study, we report the application of a simple previously described method for screening of different plant seeds in order to find the best resources of plant antimicrobial peptides. | 2011 | 22347591 |
| perennial-like adaptation mechanisms of annual legumes to limited irrigation. | the hydrodynamic behavior of two annual legumes (trifolium angustifolium l. and onobrychis caput-galli (l.) lam.) under water shortage was studied in a rain shelter experiment. seeds were collected from natural grasslands of northern greece and were sown in pots. two months after seedlings' emergence, full irrigation (up to field capacity) and limited irrigation (40% of field capacity) were applied. during the vegetative period the leaf water potential and the relative water content were measure ... | 2010 | 21047003 |
| pathogenicity of ditylenchus dipsaci to sainfoin (onobrychis viciaefolia scop.). | | 1975 | 19308139 |
| plant enemy-derived elicitors increase the foliar tannin concentration of onobrychis viciifolia without a trade-off to growth. | molecular experiments suggest that the regulation of the biosynthesis of condensed tannin (ct) is sensitive to the presence of plant enemies. the enemy-specific response of ct concentrations to simulated attacks by pathogenic fungi, bacteria or herbivores was studied in onobrychis viciifolia grown at four levels of nutrient availability. it was hypothesized that ct concentrations increase in response to an attack, and that constitutive and induced levels of ct are higher at low than at high nutr ... | 2008 | 18845664 |
| regeneration of intergeneric somatic hybrids by protoplast fusion between onobrychis viciaefolia and medicago sativa. | protoplast fusion was induced between sainfoin and alfalfa by an improved polyethyleneglycol (peg) method. the intergeneric somatic calluses were selected based on complementation of hydroxyproline-resistance of sainfoin and hormone autonomy growth of alfalfa transformation cell line. 17 somatic hybrid plantlets were regenerated. peg could induce the tight agglutination of protoplasts. during diluting and washing process, cyclization of the linked membrane and formation of vesicle-like structure ... | 1997 | 18762875 |
| the effect of dietary sainfoin ( onobrychis viciifolia) on local cellular responses to trichostrongylus colubriformis in sheep. | the effect of sainfoin (onobrychis viciifolia) hay consumption on the pathophysiology and local cellular responses of growing lambs during infection with trichostrongylus colubriformis was investigated. thirty-two lambs, 16 weeks of age, were allocated to 1 of 4 treatment groups (n=8) that were offered either grass (g) or sainfoin (s) hay while concurrently either infected (+), or not (-) with 12,000 l3 t. colubriformis larvae per week for 6 weeks. liveweight gains were affected by diet (p=0.002 ... | 2008 | 18561870 |
| effect of feeding dehydrated and ensiled tanniferous sainfoin (onobrychis viciifolia) on nitrogen and mineral digestion and metabolism of lambs. | the effects of tanniferous sainfoin on digestion and metabolism have been investigated in 12 lambs in an incomplete cross-over design (n = 6). effects of condensed tannins (ct) were evaluated by comparing dehydrated and ensiled sainfoin treated with and without polyethylene glycol (peg). dehydrated and ensiled grass-clover mixtures served as controls. the lambs were fed the treatment diets, including a mineral supplement, for 21 d. during the last 7 d excreta, rumen fluid and blood were sampled. ... | 2007 | 18030920 |
| cytotoxic effects of 2-arylbenzofuran phytoestrogens on human cancer cells: modulation by adrenal and gonadal steroids. | although 2-arylbenzofuran phytoalexins are known for decades, their anticancer activity has not been studied systematically. we have previously reported on the isolation and the estrogen receptor (er) modulation properties of three new 2-arylbenzofurans from onobrychis ebenoides, ebenfuran i [2-(2,4-dihydroxyphenyl)-5-hydroxy-6-methoxy-benzofuran], ebenfuran ii [2-(2,4-dihydroxyphenyl)-3-formyl-4-hydroxy-6-methoxy-benzofuran] and ebenfuran iii [2-(2,4-dihydroxyphenyl)-3-formyl-4-hydroxy-6-methox ... | 2007 | 17451940 |
| [responses of onobrychis viciaefolia scop and soil nitrogen contents to elevated atmospheric co2 concentration]. | the effects of elevated co2 on terrestrial ecosystem processes are highly related to the n cycling in the ecosystem. in two closed chambers with different co2 concentration (ambient co2 355 - 370 micromol x mol(-1), and elevated co2 700 micromol x mol(-1)), pot experiments were conducted to study the responses of onobrychis viciaefolia scop and soil nitrogen contents to elevated co2. the results showed that compared with ambient co2, elevated co2 increased the plant dry biomass by 25.1% (p < 0.0 ... | 2006 | 17269349 |
| evaluation of estrogenic/antiestrogenic activity of onobrychis ebenoides extract - interaction with estrogen receptor subtypes eralpha and erbeta. | a protective effect of plant extract from onobrychis ebenoides on ovariectomy-induced bone loss in rats has been shown. to investigate the molecular mechanisms that underly the beneficial effect of o. ebenoides (onb) on bone loss, we studied its potential to activate er subtypes (eralpha and erbeta) on transiently transfected hela cells with ho-heralpha or psg5-herbeta and 3xere-tata-luc expression vectors. its impact to stimulate differentiation and mineralization of osteoblasts (ks483 cell lin ... | 2007 | 17092687 |
| estrogenic activity of isoflavonoids from onobrychis ebenoides. | fractionation of the neutral extract of onobrychis ebenoides (leguminosae) yielded a new isoflavone, named ebenosin (1), in addition to the known ones, afrormosin (2), formononetin (3) and daidzein (4). although the relative binding affinities of 1 - 4 for estrogen receptor alpha (eralpha) were nearly comparable and matched those of 1-3 for erbeta, that of 4 for the latter receptor was significantly higher than any of the other. compounds 1 - 4 induced cell proliferation and gene expression in b ... | 2006 | 16773531 |
| effects of sainfoin (onobrychis viciifolia scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. | sainfoin leaf condensed tannins inhibited growth and protease activity in butyrivibrio fibrisolvens a38 and streptococcus bovis 45s1 but had little effect on prevotella ruminicola b(1)4 or ruminobacter amylophilus wp225. tannins bound to cell coat polymers in all strains. morphological changes in b. fibrisolvens and s. bovis implicated the cell wall as a target of tannin toxicity. | 1994 | 16349244 |
| protective effect of plant extract from onobrychis ebenoides on ovariectomy-induced bone loss in rats. | certain plant extracts have been the object of recent studies due to their mild estrogenic action and their possible potential role in osteoporosis prevention and/or treatment. the present study was undertaken to investigate the possible protective effect of the aqueous solution of the plant onobrychis ebenoides, with proven in vitro mild estrogenic action, on bone mass loss of the ovariectomized (ovx) rat experimental model of osteoporosis. | 2006 | 15979258 |
| testing for direct anthelmintic effects of bioactive forages against trichostrongylus colubriformis in grazing sheep. | the aim of the present study was to investigate potential direct anthelmintic effects of forages that contain plant secondary metabolites (psm) towards the intestinal nematode trichostrongylus colubriformis. for this purpose, we introduced an experimental design, which enabled us to investigate the direct anthelmintic effects of psm-rich forages, without significant interference of possible indirect immunological effects of psm. sixty parasite naive sheep were infected with 8000 t. colubriformis ... | 2004 | 15710524 |
| plant 2-arylobenzofurans demonstrate a selective estrogen receptor modulator profile. | we have isolated from the plant onobrychis ebenoides three novel arylobenzofurans with binding affinity for the estrogen receptor. in this study, we evaluated these arylobenzofurans, namely ebenfuran i, ebenfuran ii and ebenfuran iii for their potential selective estrogen receptor modulator (serm)-like properties. we examined their ability, (1) to induce the insulin growth factor binding protein-3 (igfbp-3) in mcf-7 breast cancer cells, (2) to stimulate differentiation and mineralization of oste ... | 2004 | 15579325 |
| [selection and identification of salt tolerant line of sainfoin from the seeds of first post-flight plants]. | seeds of sainfoin (onobrychis vicaefolia scop.) were carried in the recoverable satellite 940703 and recovered from earth orbit from china in 1994. the progeny seeds were obtained by producing in field. the salt tolerant calluses were selected by screening seedling and callus on 1.5% nacl-containing medium, reviving growth on nacl- free medium and selecting callus on 1.2% nacl-containing medium. the salt tolerant line callus maintained the normal ability to regenerate plant. the salt tolerant li ... | 2001 | 12549005 |
| potential maternal effects of elevated atmospheric co(2) on development and disease severity in a mediterranean legume. | global change can greatly affect plant populations both directly by influencing growing conditions and indirectly by maternal effects on development of offspring. more information is needed on transgenerational effects of global change on plants and their interactions with pathogens. the current study assessed potential maternal effects of atmospheric co(2) enrichment on performance and disease susceptibility of first-generation offspring of the mediterranean legume onobrychis crista-galli. moth ... | 2011 | 22639588 |
| multi-walled сarbon nanotubes penetrate into plant cells and affect the growth of onobrychis arenaria seedlings. | engineered nanoparticles (enps) are now being used in many sectors of industry; however, the impact of enps on the environment still requires further study, since their use, recycling, and accidental spill can result in the accumulation of nanoparticles in the atmosphere, soil, and water. plants are an integral part of ecosystems; hence their interaction with enps is inevitable. it is important to understand the consequences of this interaction and assess its potential effects. the present resea ... | 2011 | 22649678 |
| direct and indirect effects of bioactive tannin-rich tropical and temperate legumes against nematode infections. | parasitic infections with gastrointestinal nematodes (gins) represent a major pathological threat associated with the outdoor production of various livestock species. up to now, the control of these parasitic diseases essentially relied on the use of commercial anthelmintic drugs. however, resistance to anthelmintics is nowadays widespread in worm populations. recent results indicate that bioactive tanniniferous plants represent a valuable option as an alternative to commercial drugs for the con ... | 2011 | 22188981 |