| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| [nutritive value of lupine and its potential as human food]. | the chemical composition and some indices of protein quality were measured in two species of sweet lupine lupinus albus and lupinus luteus grown at the experimental station in gorbea, chile: both samples showed a high protein content (39.5 and 44.6%). the protein efficiency ratio (per) was measured in the rat and found to be 0.48 and 0.71, respectively, as compared to 2.57 for casein. supplementation with 0.3% dl-methionine increased significantly those values, thus indicating that lupine protei ... | 1979 | 550741 |
| [the lupine, a contribution to nutrition in the andes. 2. the quality of proteins in lupinus mutabilis compared to lupinus albus, lupinus luteus and soja max]. | | 1975 | 1224691 |
| isolation and characterization of peroxy-y base from phenylalanine transfer ribonucleic acid of the plant, lupinus luteus. | | 1974 | 4464312 |
| electron microscopy of the bacteroids and root nodules of lupinus luteus. | | 1965 | 5861290 |
| in situ hybridization of two nodule-specific gene products from yellow lupin. | we have applied in situ hybridization technique to examine the expression of two tissue-specific plant genes represented by cdna clones: pln-13 and pln-50 during the symbiosis of lupinus luteus with bradyrhizobium lupini. both genes are expressed in root nodules and their transcripts are restricted to the infected zone of the nodule as revealed with 35s-or 3h-labelled antisense rna probes. the number of grains over bacteroid-containing cells after hybridization with 3h-labelled probes differed d ... | 1994 | 7843467 |
| isolation and classification of a family of cyclin gene homologues in lupinus luteus. | the lupine (lupinus luteus cv. ventus) cdna clones encoding homologues of cyclin (cycb1;2, cycb1;3, cycb1;4) have been isolated from cdna library prepared from roots inoculated with bradyrhizobium lupini. comparison of the deduced amino-acid sequences of cycb1;2, cycb1;3, cycb1;4 and previously described cycb1;1 (deckert et al. 1996, biochimie 78, 90-94) showed that they share 46-65% of identical amino acids. the presence of conserved residues (renaudin et. al., in the plant cell cycle, in the p ... | 1997 | 9241352 |
| yellow lupine gene encoding stearoyl-acp desaturase--organization, expression and potential application. | a gene for the delta9 desaturase specific to stearoyl-acp (acyl carrier protein) was identified from yellow lupine (lupinus luteus) cdna and genomic libraries through the differential display method. the desaturase transcript appears in plants infected with bradyrhizobium sp. (lupinus) as revealed by northern hybridization, rt-pcr and expression of beta-glucuronidase under the desaturase promoter. a small amount of desaturase transcript was also detected in uninfected plants, which suggests that ... | 2002 | 12136953 |
| the isolation of lupine cdna clone coding for putative cyclin protein. | the complex of p34cdc2 protein kinase and cyclin is a key regulator of eukaryotic cell division and has been mostly investigated in yeast and animals. we have isolated a cdna clone corresponding to cyclin from yellow lupine (lupinus luteus). the cdna clone cycbla-ll is 1692 bp in length and encodes a protein of predicted molecular mass 48.7 kda. the lupine cyclin-like clone contains two domains: destruction and cyclin box, responsible for the function during the cell cycle. the amino acid compar ... | 1996 | 8818215 |
| expression of lupinus luteus nodulin-45 gene in e. coli cells. mutagenesis of coding sequence by pcr method. | | 1994 | 7976014 |
| chemical composition, nutritive value, and toxicological evaluation of two species of sweet lupine (lupinus albus and lupinus luteus). | | 1980 | 7391375 |
| substrate specificity of s-adenosylhomocysteinase. cysteine is a substrate of the plant and mammalian enzymes. | substrate specificity of s-adenosylhomocysteinases (s-adenosyl-l-homocysteine hydrolase, ec 3.3.1.1) with respect to amino acid has been studied using homogeneous preparations of the enzymes from yellow lupin (lupinus luteus) seeds and bovine liver. both enzymes use cysteine, in addition to homocysteine, as a substrate. homoserine, serine, pinicillamine, reduced glutathione and 2-mercaptoethanol are not substrates. in the presence of cysteine, the reaction of s-adenosylthio-amino acid synthesis ... | 1983 | 6824685 |
| chemical and nutritional evaluation of sweet lupines. | sweet lupines are presently being cultivated at the experimental station in gorbea (chile). four cultivars, lupinus albus cv astra, l. albus cv multolupa, lupinus luteus cv aurea and lupinus angustifolius, were examined for the chemical composition of whole and dehulled seeds and testa. whole seeds contain 34-35% protein. the varieties of l. albus contain 13% oil. l. luteus and l. angustifolius contain about 5% ether extract. crude fiber ranged from 11 to 17% in the whole seed. unsaturated fatty ... | 1983 | 6651228 |
| mitochondrial transfer rna genes from fungi (aspergillus nidulans) and plants (lupinus luteus) are transcribed in xenopus laevis oocyte nuclei. | three plasmids containing aspergillus nidulans mitochondrial transfer rna genes were microinjected into the nucleus of xenopus laevis oocytes. plasmids that contained a single trnacys gene or two trna genes (arg and asn) yielded trna-sized transcripts. plasmids containing cloned lupinus mitochondrial trna genes were also transcribed and processed in x. laevis nuclei. | 1983 | 6350603 |
| isolation and characterization of root nodule proteins from lupin. | a group of root nodule-specific plant proteins (nodulins) has been isolated from yellow lupin (lupinus luteus) by immunoaffinity chromatography. the cytoplasmic nodule protein extract was initially enriched in nodulins on a column with immobilized igg fraction. it was then purified by chromatography on sepharose 4b - bound igg against uninfected root proteins and finally on sepharose 4b - bound igg against rhizobium lupini proteins. rocket immunoelectrophoresis showed that the nodulin preparatio ... | 1988 | 3176786 |
| anaerobic processes in yellow lupin (lupinus luteus l.) root nodules. | the lupin root nodule homogenate was separated by centrifugation in the percoll density gradient into the rhizobium bacteroid fraction and plant subcellular components. high activities of alcohol dehydrogenase and lactate dehydrogenase in the soluble fraction of host plant, and high capability of the isolated bacteroids to oxidize ethanol, malate, lactate and acetaldehyde evidence functional interrelationship between the plant and bacteroids. | 1989 | 2486002 |
| p alpha-chiral phosphorothioate analogues of bis(5'-adenosyl)tetraphosphate (ap4a); their enzymatic synthesis and degradation. | synthesis of sp and rp diastereomers of ap4a alpha s has been characterized in two enzymatic systems, the lysyl-trna synthetase from escherichia coli and the ap4a alpha, beta-phosphorylase from saccharomyces cerevisiae. the synthetase was able to use both (sp)atp alpha s and (rp)atp alpha s as acceptors of adenylate thus yielding corresponding monothioanalogues of ap4a,(sp) ap4a alpha s and (rp)ap4a alpha s. no dithiophosphate analogue was formed. relative synthetase velocities of the formation ... | 1990 | 2172926 |
| quantitation of cytokinins in biological samples using antibodies against zeatin riboside. | the cross-reactivity of antibodies elicited in rabbits against zeatin riboside, to a wide range of naturally occurring cytokinins, was examined. as well as to zeatin riboside, the antisera cross-reacted to a considerable extent with zeatin, lupinic acid, zeatin-9-glucoside, zeatin riboside 5'-monophosphate and to a much lesser, but measurable extent, with dihydrozeatin riboside and dihydrozeatin. chromatographic methods were devised which allowed separation of all these cross-reactive compounds. ... | 1984 | 16663745 |
| immobilized metal ion affinity chromatography in enzyme fractionation. | ribitol dehydrogenase from mycobacterium butyricum and alpha-mannosidase from lupinus luteus seedlings were fractionated by the immobilized metal ion (cu2+ or zn2+) affinity chromatography (imac) on iminodiacetic acid coupled to sepharose 6b. in a single step, ribitol dehydrogenase was purified 10-12 fold with the recovery above 80% when using zn(2+)-sepharose 6b as the sorbent and decreasing linear gradient of ph from 7 to 4. in the same conditions purification of alpha-mannosidase was less eff ... | 1991 | 1796691 |
| [metlegoglobin reductase from lupine nodules. purification and properties]. | the purification procedure and properties of metlegoglobin reductase from the soluble fraction of lupine (lupinus luteus l.) nodules and from the proteins secreted by bacteroids rhizobium lupini in vitro are described. the properties of both forms of enzyme were found to be similar. a metlegoglobin reductase preparation purified 125-fold with a yield of 21% was obtained. the enzyme is strictly specific to the cofactor (nadh). no substrate specificity was revealed. the enzyme reduces oxidized cyt ... | 1979 | 227483 |
| selective splitting of 3'-adenylated dinucleoside polyphosphates by specific enzymes degrading dinucleoside polyphosphates. | several 3'-[(32)p]adenylated dinucleoside polyphosphates (np(n)n'p*as) were synthesized by the use of poly(a) polymerase (sillero mag et al., 2001, eur j biochem.; 268: 3605-11) and three of them, apppa[(32)p]a or apppap*a, appppap*a and gppppgp*a, were tested as potential substrates of different dinucleoside polyphosphate degrading enzymes. human (asymmetrical) dinucleoside tetraphosphatase (ec 3.6.1.17) acted almost randomly on both appppap*a, yielding approximately equal amounts of pppa + pap ... | 2003 | 12673352 |
| metabolism of homocysteine-thiolactone in plants. | editing of the amino acid homocysteine (hcy) by certain aminoacyl-trna synthetases results in the formation of an intramolecular thioester, hcy-thiolactone. here we show that the plant yellow lupin, lupinus luteus, has the ability to synthesize hcy-thiolactone. the inhibition of methylation of hcy to methionine by the anitifolate drug aminopterin results in greatly enhanced synthesis of hcy-thiolactone by l. luteus plants. methionine inhibits the synthesis of hcy-thiolactone in l. luteus, sugges ... | 2003 | 12496251 |
| purification, crystallization and preliminary crystallographic studies of plant s-adenosyl-l-homocysteine hydrolase (lupinus luteus). | by degrading s-adenosyl-l-homocysteine, which is a byproduct of s-adenosyl-l-methionine-dependent methylation reactions, s-adenosyl-l-homocysteine hydrolase (sahase) acts as a regulator of cellular methylation processes. s-adenosyl-l-homocysteine hydrolase from the leguminose plant yellow lupin (lupinus luteus), llsahase, which is composed of 485 amino acids and has a molecular weight of 55 kda, has been cloned, expressed in escherichia coli and purified. crystals of llsahase in complex with ade ... | 2008 | 18607106 |
| diphosphonucleotide phosphatase/phosphodiesterase from yellow lupin (lupinus luteus l.) belongs to a novel group of specific metallophosphatases. | a cdna encoding previously purified and characterized diphosphonucleotide phosphatase/phosphodiesterase (ppd1) from yellow lupin (lupinus luteus l.) was identified. the ppd1 gene encodes a protein containing a cleavable signal sequence. a functional expression of ppd1 in saccharomyces cerevisiae confirmed the proper gene identification. a gene homologous to ppd1, encoding a putative membrane protein (ppd2), as well as fragments of two other genes encoding ppd3 and ppd4 proteins were also isolate ... | 2002 | 12023036 |
| the root-nodule symbiosis between sarothamnus scoparius l. and its microsymbionts. | when nitrogen fixing root nodules are formed, sarothamnus scoparius (broom) is inoculated with its microsymbionts. nodules studied under light and electron microscopy exhibited typical indeterminate nodule histology with apical, persistent meristem, age gradient of nodule tissues, and open vascular bundles, and also with some particular features such as: the presence of mitotic activity in the infected meristematic cells, lack of infection threads, distribution of bacteria by process of host cel ... | 2001 | 11816984 |
| characterization and expression analysis of the yellow lupin (lupinus luteus l.) gene coding for nodule specific proline-rich protein. | the llprp2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of enod2 nodule-specific genes. several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the llprp2 gene. northern blot analysis revealed that the expression of the llprp2 gene begins 9 days after inoculation of yellow lupin roots with bradyrhizobium sp. (lupinus); the expression is restricted to symbiotic ... | 2000 | 11051202 |
| diverse accumulation of several dehydrin-like proteins in cauliflower (brassica oleracea var. botrytis), arabidopsis thaliana and yellow lupin (lupinus luteus) mitochondria under cold and heat stress. | dehydrins represent hydrophilic proteins acting mainly during cell dehydration and stress response. dehydrins are generally thermostable; however, the so-called dehydrin-like (dehydrin-related) proteins show variable thermolability. both groups immunoreact with antibodies directed against the k-segment of dehydrins. plant mitochondrial dehydrin-like proteins are poorly characterized. the purpose of this study was to extend previous reports on plant dehydrins by comparing the level of immunopreci ... | 2010 | 20718974 |
| hypochlorous acid-induced lysis of human erythrocytes. inhibition of cellular damage by the isoflavonoid genistein-8-c-glucoside. | erythrocyte damage induced by hypochlorous acid (hocl) results in cell lysis developing with time after the oxidant is removed (post-hemolysis). the apparent rate constant of post-hemolysis depends on time of incubation in the presence of hocl and concentration of this oxidant. hocl-dependent damage of erythrocyte membranes is associated with uncompetitive inhibition of the membrane-bound acetylcholinesterase. genistein-8-c-glucoside is an isoflavonoid isolated from the flowers of lupinus luteus ... | 2000 | 11002188 |
| crystallization and preliminary x-ray structure determination of lupinus luteus pr10 protein. | the pathogenesis-related protein of the pr10 class from lupinus luteus (yellow lupin), llpr10.1a, is constitutively expressed in roots. it is also accumulated in leaves treated with a suspension of pathogenic bacteria as a response to stress. recombinant yellow-lupin llpr10.1a protein has been overexpressed in escherichia coli as a fusion product with maltose-binding protein. llpr10.1a crystallizes in the orthorhombic p2(1)2(1)2(1) space group and the crystals diffract to 2.45 a resolution. the ... | 1999 | 10531497 |
| a plant-derived edible vaccine against hepatitis b virus. | the infectious hepatitis b virus represents 42 nm spherical double-shelled particles. however, analysis of blood from hepatitis b virus carriers revealed the presence of smaller 22 nm particles consisting of a viral envelope surface protein. these particles are highly immunogenic and have been used in the design of hepatitis b virus vaccine produced in yeast. upon expression in yeast, these proteins form virus-like particles that are used for parenteral immunization. therefore, the dna fragment ... | 1999 | 10506582 |
| site-directed mutagenesis of histidine 69 and glutamic acid 148 alters the ribonuclease activity of pea abr17 (pr10.4). | pea abscisic acid responsive (abr17) protein is a member of the pathogenesis-related 10 (pr10) family of proteins and its ribonuclease (rnase) activity has been reported previously. in order to investigate the amino acids important for the demonstrated ribonuclease activity of abr17, site-directed mutants h69l and e148a were generated, expressed in escherichia coli and purified to homogeneity. these mutations affected rnase activity differently; the h69l mutant exhibited a decreased rnase activi ... | 2011 | 21600779 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| molecular cloning of lupin leghemoglobin cdna. | poly(a)+ rna isolated from root nodules of yellow lupin (lupinus luteus, var. ventus) has been used as a template for the construction of a cdna library. the ds cdna was synthesized and inserted into the hind iii site of plasmid pbr 322 using synthetic hind iii linkers. clones containing sequences specific for nodules were selected by differential colony hybridization using 32p-labeled cdna synthesized either from nodule poly(a)+ rna or from poly(a)+ rna of uninfected root as probes. among the r ... | 2008 | 2442601 |
| the non-enzymatic hydrolysis of oligoribonucleotides vi. the role of biogenic polyamines. | single-stranded oligoribonucleotides containing ua and ca phosphodiester bonds can be hydrolyzed specifically under non-enzymatic conditions in the presence of spermidine, a biogenic amine found in a wide variety of organisms. in the present study, the rate of oligonucleotide and trna(i)(met)hydrolysis was measured in the presence of spermidine and other biogenic amines. it was found that spermine [h(3)n(+)(ch(2))(3)(+)nh(2)(ch(2))(4)(+)nh(2)(ch(2))(3)(+)nh(3)] and putrescine [h(3)n(+)(ch(2))(4) ... | 1999 | 10481033 |
| modular evolution of the glx-trna synthetase family--rooting of the evolutionary tree between the bacteria and archaea/eukarya branches. | the accuracy of protein biosynthesis generally rests on a family of 20 aminoacyl-trna synthetases, one for each amino acid. in bacteria, archaea and eukaryotic organelles, the formation of gln-trna(gln) is prevalently accomplished by a transamidation pathway, aminoacylation of trna(gln) with glu by glutamyl-trna synthetase (glurs) followed by a trna-dependent transamidation of glu from glu-trna(gln). a few bacterial species, such as escherichia coli, possess a glutaminyl-trna synthetase (glnrs), ... | 1998 | 9746349 |
| interaction of hiv tat model peptides with trna and 5s rrna. | new data are presented on the interaction of model synthetic peptides containing an arginine-rich region of human immunodeficiency virus (hiv-tat), with native rna molecules: trna(phe) of saccharomyces cerevisiae and 5s rrna from lupinus luteus. both rna species form complexes with the tat1 (grkkrrqrrra) and tat2 (grkkrrqrrrapqdsqthqaslskqpa) peptides, as shown by electrophoretic gel shift and rnase footprint assays, and cd measurements. the nucleotide sequence uggg located in the dihydrouridine ... | 1997 | 9511968 |
| expression of lupinus luteus cdna coding for pr10 protein in escherichia coli: purification of the recombinant protein for structural and functional studies. | the cdna clones coding for two pathogenesis-related protein homologues of pr10 class, llpr10.1a and llpr10.1b, were identified in yellow lupin expression library of uninfected roots. the contribution of pr10 proteins to the overall mechanism of plant defence still remains unknown. in order to elucidate the structure and function of lupin pr10.1a protein, a substantial quantity of the protein was produced in an e. coli expression system using plasmids of pet-series: pet-3a and pet-15b, carrying t ... | 1997 | 9511966 |
| expression of the cdna and purification of p0 ribosomal protein from lupinus luteus. | eukaryotic ribosomal protein p0 is localized on the large ribosomal subunit at the base of the specific "stalk" structure, close to the central protuberance. recently we have obtained a clone coding for p0 ribosomal protein from l. luteus (yellow lupin) cdna library. here we present its expression in e. coli cells and purification of the target protein. | 1998 | 9503149 |
| cloning and expression of diadenosine 5',5'''-p1,p4-tetraphosphate hydrolase from lupinus angustifolius l. | the first isolation, cloning and expression of cdna encoding an asymmetric diadenosine 5',5'''p1,p4-tetraphosphate pyrophosphohydrolase (ap4a hydrolase) from a higher plant is described. ap4a hydrolase protein was purified from seeds of both lupinus luteus and lupinus angustifolius and partially sequenced. the ap4a hydrolase cdna was cloned from l. angustifolius cotyledonary polyadenylated rna using reverse transcription and pcr with primers based on the amino acid sequence. the cdna encoded a p ... | 1998 | 9425114 |
| effect of a short-term hypoxic treatment followed by re-aeration on free radicals level and antioxidative enzymes in lupine roots. | to investigate whether re-aeration after a short-term hypoxic pre-treatment (for 2, 12 or 24 h) induces oxidative stress, the temporal sequence of physiological reactions, including the level of free radicals, hydrogen peroxide production, and changes in antioxidative enzymes, was characterized in roots of hydroponically grown lupine (lupinus luteus l., cv. juno) seedlings. by using electron paramagnetic resonance (epr), we found that the exposure of hypoxically grown roots (hypoxic pre-treatmen ... | 2004 | 15051047 |
| cytokinin stimulates polyribosome loading of nuclear-encoded mrnas for the plastid atp synthase in etioplasts of lupinus luteus: the complex accumulates in the inner-envelope membrane with the cf(1) moiety located towards the stromal space. | three of the nine subunits of the plastid atp synthase, including the subunit of the cf(1) moiety (gene atpc), are encoded in the nucleus. application of cytokinin to etiolated lupine seedlings induces polyribosome association of their mrnas. this appears to be specific as no such regulation was observed for messages for three ribosomal proteins. cytokinin-mediated polyribosome loading was also observed for the spinach atpc message in etiolated transgenic tobacco seedlings. analysis of various s ... | 2004 | 15125765 |
| re-aeration-induced oxidative stress and antioxidative defenses in hypoxically pretreated lupine roots. | the level of free radicals and activities of antioxidative enzymes were examined in roots of lupine seedlings (lupinus luteus l.) that were deprived of oxygen by subjecting them to root hypoxia for 48 and 72 h and then re-aerated for up to 24 h. using electron paramagnetic resonance (epr), we found that the exposure of previously hypoxically grown roots to air caused the increase in free radicals level, irrespective of duration of hypoxic pretreatment. immediately after re-aeration the level of ... | 2004 | 15128029 |
| response of embryo axes of germinating seeds of yellow lupine to fusarium oxysporum. | defence responses of embryo axes of lupinus luteus l. cv. polo were studied 48-96 h after inoculation with fusarium oxysporum schlecht f.sp. lupini. the infection restricted the growth of embryo axes, the lengths of infected embryo axes 72 and 96 h after inoculation were 11 and 12 mm less in the controls, respectively, while their masses c. 0.03 g less than in the controls. the concentration of h2o2 in embryo axes of inoculated germinating seeds was higher than in the control. this was probably ... | 2004 | 15246062 |
| expression, purification and catalytic activity of lupinus luteus asparagine beta-amidohydrolase and its escherichia coli homolog. | we describe the expression, purification, and biochemical characterization of two homologous enzymes, with amidohydrolase activities, of plant (lupinus luteus potassium-independent asparaginase, lla) and bacterial (escherichia coli, ybik/spt/iaaa gene product, ecaiii) origin. both enzymes were expressed in e. coli cells, with (lla) or without (ecaiii) a his-tag sequence. the proteins were purified, yielding 6 or 30 mg.l(-1) of culture, respectively. the enzymes are heat-stable up to 60 degrees c ... | 2004 | 15265041 |
| sucrose-induced lupine defense against fusarium oxysporum. sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to fusarium oxysporum. | defense responses to inoculation with fusarium oxysporum schlecht f. sp. lupini were studied in embryo axes of lupinus luteus l. cv. polo cultured on a medium with sucrose (60 mm) or without it. exogenous sucrose caused a marked endogenous increase in concentrations of sucrose, glucose and fructose in embryo axes. in axes cultured with sucrose, high performance liquid chromatography (hplc) revealed generally higher levels of isoflavone glycosides (particularly until 48 h of culture) and free agl ... | 2005 | 15907688 |
| the possible involvement of peroxidase in defense of yellow lupine embryo axes against fusarium oxysporum. | peroxidase activity (ec 1.11.1.7) towards phenolic substrates, i.e. pyrogallol, syringaldazine and guaiacol, and ascorbate peroxidase activity (ec 1.11.1.11) were analyzed in embryo axes of lupinus luteus l. cv. polo cultured on heller medium for 96h after inoculation with the necrotrophic fungus fusarium oxysporum f.sp. schlecht lupini. four variants were compared: inoculated embryo axes cultured with 60mm sucrose (+si) or without it (-si), and non-inoculated embryo axes cultured with 60mm sucr ... | 2007 | 16376454 |
| a model for predicting ionic equilibrium concentrations in cell walls. | purified cell walls were prepared from roots of horse bean (vicia faba l., var. minor) and yellow lupine (lupinus luteus l.). two methods were used: (a) grinding and (b) lysis of the endocellular contents by triton x-100. the ca(2+), mg(2+), and k(+) contents were determined after incubation in various solutions in such a manner that the measurements gave access to the undisturbed equilibrium contents. the results were used to test a model which describes the ionic atmosphere in the cell walls a ... | 1981 | 16661927 |
| occurrence of h(2)-uptake hydrogenases in bradyrhizobium sp. (lupinus) and their expression in nodules of lupinus spp. and ornithopus compressus. | fifty-four strains of bradyrhizobium sp. (lupinus) from worldwide collections were screened by a colony hybridization method for the presence of dna sequences homologous to the structural genes of the bradyrhizobium japonicum hydrogenase. twelve strains exhibited strong colony hybridization signals, and subsequent southern blot hybridization experiments showed that they fell into two different groups on the basis of the pattern of ecori fragments containing the homology to the hup probe. all str ... | 1989 | 16666550 |
| fusarium oxysporum-induced oxidative stress and antioxidative defenses of yellow lupine embryo axes with different sugar levels. | this study was designed to investigate whether and to what extent oxidative stress is induced in embryo axes of lupinus luteus l. cv. polo inoculated with a necrotrophic fungus, fusarium oxysporum and cultured on heller medium for 96h. four variants were compared: inoculated embryo axes cultured with 60mm sucrose (+si) or without it (-si), and non-inoculated embryo axes cultured with 60mm sucrose (+sn) or without it (-sn). after inoculation, an accumulation of stable free radicals and mn2+ ions ... | 2008 | 17913293 |
| ability of lupine seeds to germinate and to tolerate desiccation as related to changes in free radical level and antioxidants in freshly harvested seeds. | seeds of yellow lupine (lupinus luteus l. cv. juno) were collected throughout their development on the mother plant to determine whether the ability to germinate and to tolerate desiccation is related to the level of free radicals and the changes in the redox state of ascorbate and glutathione as well as the activities of antioxidative enzymes. electron paramagnetic resonance (epr)-based analyses showed that development of lupine seed was accompanied by generation of free radicals with g(1) and ... | 2009 | 18945622 |
| effect of genistein-8-c-glucoside from lupinus luteus on dna damage assessed using the comet assay in vitro. | genistein-8-c-glucoside (g8cg) belongs to natural isoflavones phytoestrogens, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants, with possible anticarcinogenic effects in various in vitro systems and in vivo animal models. we used glycosylated genistein (genistein-8-c-glucoside) from flowers of lupine (lupinus luteus l.) to study its cytotoxic and genotoxic effects on mouse embryonic fibroblast (line nih 3t3). the mtt assay to assess cytotox ... | 2009 | 19059355 |
| "in situ" phytostabilisation of heavy metal polluted soils using lupinus luteus inoculated with metal resistant plant-growth promoting rhizobacteria. | the aim of this work is the evaluation of metal phytostabilisation potential of lupinus luteus inoculated with bradyrhizobium sp. 750 and heavy metal resistant pgprs (plant-growth promoting rhizobacteria), for in situ reclamation of multi-metal contaminated soil after a mine spill. yellow lupines accumulated heavy metals mainly in roots (cu, cd and especially pb were poorly translocated to shoots). this indicates a potential use of this plant in metal phytostabilisation. furthermore, as accumula ... | 2010 | 20056325 |
| cross-talk interactions of sucrose and fusarium oxysporum in the phenylpropanoid pathway and the accumulation and localization of flavonoids in embryo axes of yellow lupine. | this study investigated the effects of cross-talk interactions of sucrose and infection caused by a pathogenic fungus fusarium oxysporum f.sp. lupini on the regulation of the phenylpropanoid pathway, i.e. the level of expression of genes encoding enzymes participating in flavonoid biosynthesis, as well as cell location and accumulation of these compounds in embryo axes of lupinus luteus l. cv. polo. embryo axes, both non-inoculated and inoculated, were cultured for 96h on heller medium with 60mm ... | 2010 | 21056513 |
| complete genome sequencing of agrobacterium sp. h13-3, the former rhizobium lupini h13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing ti-plasmid. | agrobacterium sp. h13-3, formerly known as rhizobium lupini h13-3, is a soil bacterium that was isolated from the rhizosphere of lupinus luteus. the isolate has been established as a model system for studying novel features of flagellum structure, motility and chemotaxis within the family rhizobiaceae. the complete genome sequence of agrobacterium sp. h13-3 has been established and the genome structure and phylogenetic assignment of the organism was analysed. for de novo sequencing of the agroba ... | 2011 | 21329740 |
| plant nucleoside 5'-phosphoramidate hydrolase; simple purification from yellow lupin (lupinus luteus) seeds and properties of homogeneous enzyme. | adenosine 5'-phosphoramidate (nh2-pa) is an uncommon natural nucleotide of poorly understood biochemistry and function. we studied a plant enzyme potentially involved in the catabolism of nh2-pa. a fast and simple method comprising extraction of yellow lupin (lupinus luteus) seed-meal with a low ionic strength buffer, ammonium sulfate and acetone fractionations, removal of contaminating proteins by heat denaturation, and affinity chromatography on amp-agarose, yielded homogenous nucleoside 5'-ph ... | 2011 | 21403921 |
| identification and differential distribution of cart in the small intestine depending on the diet. | this study was aimed at identifying and locating cocaine- and amphetamine-regulated transcript (cart) in the small intestine of broilers in relation to the diet. the feeding regime of the chicks was based on diets largely consisting of maize and one of four protein sources: post-extraction soya bean meal (sbm) or non-gm seed meal - meal from traditional variety of soy seeds glicine max (ffs) and meal from seeds of lupinus angustifolius (la) and lupinus luteus l (ly). the presence of cocaine- and ... | 2014 | 24797515 |
| the plant aminoacyl-trna synthetases. purification and characterization of valyl-trna, tryptophanyl-trna and seryl-trna synthetases from yellow-lupin seeds. | valyl-trna, tryptophanyl-trna, and seryl-trna synthetases from yellow lupin seeds lupinus luteus were purified to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on aminohexyl-sepharose column and affinity chromatography on trna-sepharose column. valyl-trna synthetase consists of one polypeptide chain of molecular weight 125000 as judged by sephadex g-200 gel filtration and dodecylsulfate-polyacrylamide gel electrophoresis in the presence of reducing agent. seryl-trna s ... | 2003 | 240681 |
| nucleotide sequence of trnaphe from the seeds of lupin (lupinus luteus). comparison of the major species with wheat germ trnaphe. | the nucleotide sequence of trnaphe of yellow lupin seeds (lupinus luteus) is deduced from the composition of pancreatic and t1 ribonuclease digestion products and compared with trnaphe of wheat germ. major lupin trnaphe, unlike pea trnaphe, differs from wheat germ trnaphe in the first base pair of stem tpsic ("e"). | 1994 | 264239 |
| nitrate uptake, nitrate reductase distribution and their relation to proton release in five nodulated grain legumes. | nitrate uptake, nitrate reductase activity (nra) and net proton release were compared in five grain legumes grown at 0.2 and 2 mm nitrate in nutrient solution. nitrate treatments, imposed on 22-d-old, fully nodulated plants, lasted for 21 d. increasing nitrate supply did not significantly influence the growth of any of the species during the treatment, but yellow lupin (lupinus luteus) had a higher growth rate than the other species examined. at 0.2 mm nitrate supply, nitrate uptake rates ranged ... | 2002 | 12234143 |
| a comparative study of phase states of the peribacteroid membrane from yellow lupin and broad bean nodules. | a comparative study of the lipid bilayer phase status and structure of the outer membrane of free-living bradyrhizobium strain 359a (nod(+)fix(+)) and 400 (nod(+)fixl) or rhizobium leguminosarum 97 (nod(+)fix(+), effective) and 87 (nod(+)fixl, ineffective) has been carried out. also, the effect of the symbiotic pair combination on the lipid bilayer structure of the bacteroid outer membrane and peribacteroid membrane, isolated from the nodules of lupinus luteus l. or vicia faba l., has been studi ... | 2014 | 24804101 |
| translational and structural analysis of the shortest legume enod40 gene in lupinus luteus. | two early nodulin 40 (enod40) genes, enod40-1, the shortest legume enod40 gene, and enod40-2, were isolated from lupinus luteus, a legume with indeterminate nodules. both genes were expressed at similar levels during symbiosis with nitrogen-fixing bacteria. enod40 phylogeny clustered the l. luteus genes with legumes forming determinate nodules and revealed peptide similarities. the enod40-1 small orf a fused to a reporter gene was efficiently expressed in plant cells, indicating that the start c ... | 2009 | 19287803 |
| some 2s albumin from peanut seeds exhibits inhibitory activity against aspergillus flavus. | a crude 2s albumin fraction was separated from peanut (arachis hypogaea l.) cotyledons. untreated 2s albumin had little inhibitory activity against trypsin, spore germination, or hyphal growth of aspergillus flavus. however, following treatment of 2s albumin with sds, increased inhibitory activity was demonstrated. we further purified 2s albumin using sephadex g-100 and deae cellulose (de-32) chromatography. hplc analysis showed that the partially pure 2s albumin consisted of two polypeptides, w ... | 2013 | 23500710 |
| cytisus villosus from northeastern algeria is nodulated by genetically diverse bradyrhizobium strains. | fifty-one rhizobial strains isolated from root nodules of cytisus villosus growing in northeastern algeria were characterized by genomic and phenotypic analyses. isolates were grouped into sixteen different patterns by pcr-rapd. the phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16s rrna, glnii, reca, and atpd) and one symbiotic gene (nodc). analysis of 16s rrna gene sequences showed that all the isolat ... | 2014 | 24756908 |
| effects of endogenous signals and fusarium oxysporum on the mechanism regulating genistein synthesis and accumulation in yellow lupine and their impact on plant cell cytoskeleton. | the aim of the study was to examine cross-talk interactions of soluble sugars (sucrose, glucose and fructose) and infection caused by fusarium oxysporum f.sp. lupini on the synthesis of genistein in embryo axes of lupinus luteus l.cv. juno. genistein is a free aglycone, highly reactive and with the potential to inhibit fungal infection and development of plant diseases. as signal molecules, sugars strongly stimulated accumulation of isoflavones, including genistein, and the expression of the iso ... | 2014 | 25178062 |
| cross-talk interactions of exogenous nitric oxide and sucrose modulates phenylpropanoid metabolism in yellow lupine embryo axes infected with fusarium oxysporum. | the aim of the study was to examine cross-talk of exogenous nitric oxide (no) and sucrose in the mechanisms of synthesis and accumulation of isoflavonoids in embryo axes of lupinus luteus l. cv. juno. it was verified whether the interaction of these molecules can modulate the defense response of axes to infection and development of the pathogenic fungus fusarium oxysporum f. sp. lupini. sucrose alone strongly stimulated a high level of genistein glucoside in axes pretreated with exogenous nitric ... | 2013 | 23987816 |
| synthesis and comparison of antiplasmodial activity of (+), (-) and racemic 7-chloro-4-(n-lupinyl)aminoquinoline. | recently the n-(-)-lupinyl-derivative of 7-chloro-4-aminoquinoline ((-)-am-1; 7-chloro-4-{n-[(1s,9ar)(octahydro-2h-quinolizin-1-yl)methyl]amino}quinoline) showed potent in vitro and in vivo activity against both chloroquine susceptible and resistant strains of plasmodium falciparum. however, (-)-am-1 is synthesized starting from (-)-lupinine, an expensive alkaloid isolated from lupinus luteus whose worldwide production is not sufficient, at present, for large market purposes. to overcome this is ... | 2012 | 22901673 |
| compositional changes in (iso)flavonoids and estrogenic activity of three edible lupinus species by germination and rhizopus-elicitation. | the effects of germination and elicitation on (iso)flavonoid composition of extracts from three edible lupine species (lupinus luteus, lupinus albus, lupinus angustifolius) were determined by rp-uhplc-ms(n). the total (iso)flavonoid content of lupine increased over 10-fold upon germination, with the total content and composition of isoflavonoids more affected than those of flavonoids. glycosylated isoflavones were the most predominant compounds found in lupine seedlings. lesser amounts of isofla ... | 2016 | 26749476 |
| poly(a) rnas including coding proteins rnas occur in plant cajal bodies. | the localisation of poly(a) rna in plant cells containing either reticular (allium cepa) or chromocentric (lupinus luteus, arabidopsis thaliana) nuclei was studied through in situ hybridisation. in both types of nuclei, the amount of poly(a) rna was much greater in the nucleus than in the cytoplasm. in the nuclei, poly(a) rna was present in structures resembling nuclear bodies. the molecular composition as well as the characteristic ultrastructure of the bodies containing poly(a) rna demonstrate ... | 2014 | 25369024 |
| the perichromatin region of the plant cell nucleus is the area with the strongest co-localisation of snrna and sr proteins. | the spatial organisation of the splicing system in plant cells containing either reticular (allium cepa) or chromocentric (lupinus luteus) nuclei was studied by immunolabelling of sr proteins, snrna, and the pana antigen, known markers for interchromatin granule clusters in mammalian cells. electron microscope results allowed us to determine the distribution of these molecules within the structural domains of the nucleus. similar to animal cells, in both plant species sr proteins were localised ... | 2012 | 22526497 |
| bioactivities of some essential oils against the camel nasal botfly, cephalopina titillator. | nasopharyngeal myiasis of camels is caused by the larvae of cephalopina titillator. we determined the efficacy of essential oils (eos) of pumpkin, cucurbita maxima; lupinus, lupinus luteus; garlic oil, allium sativum; and peppermint, mentha piperita, against the third larval stage of c. titillator using larval immersion tests. the positive control group was treated with ivermectin and the negative control one was treated with distilled water and few drops of tween 80. larvae were reared until ad ... | 2014 | 24276644 |
| phylogeny of nodulation genes and symbiotic properties of genista tinctoria bradyrhizobia. | pairwise comparisons of genista tinctoria (dyer's weed) rhizobium noda, nodc, and nodz gene sequences to those available in databanks revealed their highest sequence identities to nodulation loci of bradyrhizobium sp. (lupinus) strains and rhizobia from other genistoid legumes. on phylogenetic trees, genistoid microsymbionts were grouped together in monophyletic clusters, which suggested that their nodulation genes evolved from a common ancestor. g. tinctoria nodulators formed symbioses not only ... | 2006 | 16802175 |
| the effect of short-term nutritional supplementation of ewes with lupin grain (lupinus luteus), during the luteal phase of the estrous cycle on the number of ovarian follicles and the concentrations of hormones and glucose in plasma and follicular fluid. | an experiment was conducted using 16 cyclic, welsh mountain ewes during the luteal phase of the estrous cycle to determine the effect of a 5-day period of feeding a high-energy high-protein diet (lupin grain; 500 g/day) on folliculogenesis and on the plasma concentrations of glucose, insulin, follicle stimulating hormone (fsh) and estradiol-17beta, and on the follicular fluid concentrations of glucose, inhibin a, estradiol-17beta, androstenedione and progesterone. average weight did not differ b ... | 2007 | 17826826 |
| a cell-free yellow lupin extract containing activities of pseudouridine 35 and 55 synthases. | plant cytoplasmic tyrosine trna was pseudouridylated at three different positions: 35, 39 and 55. these pseudouridines were introduced by three different enzymes--pseudouridine synthases. variants of the arabidopsis thaliana pre-trna(tyr) were constructed that allow to monitor specifically pseudouridylation at different nucleotide positions. using such rnas to assay pseudouridine synthesis we have prepared an extract from lupinus luteus cv. ventus seeds containing activities of at least psi35 an ... | 1998 | 9918501 |
| cloning and sequencing of the first plant glnrs and glurs genes. | we have cloned and sequenced glutamate-trna synthetase (glurs) and glutaminyl-trna synthetase (glnrs) from arabidopsis thaliana. they have conservative motifs found in all known glxrs genes. for lupinus luteus we found only one gene of glxrs. at the moment we do not know exactly, whether it corresponds to glnrs or glurs. | 1995 | 8643358 |
| characterization of a pathogenesis-related class 10 protein (pr-10) from astragalus mongholicus with ribonuclease activity. | a pathogenesis-related (pr) class 10 protein (designated ampr-10) was first isolated from the chinese medicinal material astragalus mongholicus using a combination of affinity chromatography on zn-chelate agarose 4b, ion exchange chromatography on qae sephadex a-25 and gel filtration on sephadex g50. the purified ampr-10 showed a single band with a molecular mass of 17.2kda in sds-page. the molecular mass of intact ampr-10 was determined to be 32.8kda by gel filtration. thus, ampr-10 is a dimeri ... | 2008 | 18024144 |
| compositional variations for alpha-galactosides in different species of leguminosae, brassicaceae, and barley: a chemotaxonomic study based on chemometrics and high-performance capillary electrophoresis. | the contents of raffinose family oligosaccharides (rfo) and sucrose in brassica, lupinus, pisum, and hordeum species were investigated by chemometric principal component analysis (pca). hordeum samples contained sucrose and raffinose, and brassica samples all contained sucrose, raffinose, and stachyose. in addition to these, the pisum samples contained verbascose and the lupinus samples also contained ajugose. high stachyose and low ajugose contents were found in lupinus albus in contrast to lup ... | 2005 | 15998152 |
| effect of the phytoestrogen, genistein-8-c-glucoside, on chinese hamster ovary cells in vitro. | genistein-8-c-glucoside (g8cg) belongs to isoflavones, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants. a number of studies on flavonoids show their cardioprotective and antiosteoporosis properties in in vitro and in vivo models. as a phytoestrogen, genistein has recently generated interest as a potential anticancer and antiatherogenic agent. several flavonoids are known as antioxidants and scavengers of free oxygen radicals. in the curren ... | 2007 | 17601753 |
| structural analysis and profiling of phenolic secondary metabolites of mexican lupine species using lc-ms techniques. | flavonoid glycoconjugates from roots and leaves of eight north america lupine species (lupinus elegans, lupinus exaltatus, lupinus hintonii, lupinus mexicanus, lupinus montanus, lupinus rotundiflorus, lupinus stipulatus, lupinus sp.), three mediterranean species (lupinus albus, lupinus angustifolius, lupinus luteus) and one species from south america domesticated in europe (lupinus mutabilis) were analyzed using two lc/ms systems: low-resolution ion trap instrument and high-resolution quadrupole ... | 2013 | 23642387 |
| rhizostabilization of metals in soils using lupinus luteus inoculated with the metal resistant rhizobacterium serratia sp. msmc541. | the aim of this work was to test lupinus luteus plants, inoculated with metal resistant rhizobacteria, in order to phytostabilise metals in contaminated soils. the resistance to heavy metals of strains isolated from nodules of lupinus plants was evaluated. the strain msmc541 showed multi-resistance to several metals (up to 13.3 mm as, 2.2 mm cd, 2.3 mm cu, 9 mm pb and 30 mm zn), and it was selected for further characterization. furthermore, this strain was able to biosorb great amounts of metals ... | 2012 | 22567710 |
| determination by capillary electrophoresis of total and available niacin in different development stage of raw and processed legumes: comparison with high-performance liquid chromatography. | the available and total niacin evolution during maturation of yellow pea lupine (lupinus luteus l., cv. juno), pea (pisum sativum l., cv. ergo), faba bean (vicia faba sp. minor harz, cv. tibo) and in germinated and high-pressure heated peas (pisum sativum l., cv. esla) have been determined by capillary electrophoresis (ce). the results have been compared with those obtained by high-performance liquid chromatography (hplc). the values obtained by ce were similar to those obtained by hplc. maturat ... | 2001 | 11386659 |
| activation of phenylpropanoid pathway in legume plants exposed to heavy metals. part i. effects of cadmium and lead on phenylalanine ammonia-lyase gene expression, enzyme activity and lignin content. | species-specific changes in expression of phenylalanine ammonia-lyase (pal) and lignin content were detected in roots of soybean (glycine max l.) and lupine (lupinus luteus l.) seedlings treated with different concentrations of cadmium (cd(2+), 0-25 mg/l) or lead (pb(2+), 0-350 mg/l). the stimulatory effect of both metals was observed in mrna coding for pal in soybean. in the case of lupine, changes of pal mrna level were dependent on the metal used: cd(2+) caused a decrease, whereas pb(2+) an i ... | 2011 | 21503278 |
| distribution of n within pea, lupin, and soybean nodules. | the (15)n abundance of some, but not all, legume root nodules is significantly elevated compared to that of the whole plant. it seems probable that differences in (15)n enrichment reflect differences in the assimilatory pathway of fixed n. in that context, we have determined the distribution of naturally occurring (15)n in structural fractions of nodules from soybean (glycine max l. merr.), yellow lupin (lupinus luteus), and pea (pisum sativum) nodules and in chemical components from soybean nod ... | 1989 | 16666787 |
| the amino acid sequence of pea (pisum sativum) leghemoglobin. | the amino acid sequence has been determined for leghemoglobin component i from root nodules of pea, pisum sativum. pea leghemoglobin is one polypeptide chain composed of 147 amino acids, it contains one methionine residue at position 144, and three histidines, which are at positions 60, 92 and 101. the sequence has at least seven polymorphic residues, but it was not possible to separate the polymorphic protein forms which had identical electric charge. the approximate molecular weight of pea leg ... | 1980 | 7378476 |
| regulation of poly(a) rna retention in the nucleus as a survival strategy of plants during hypoxia. | last finding indicates that post-transcriptional processes are significant in low-oxygen conditions, but their nature is poorly understood. here, we localized poly(a) rna and mrna coding proteins involved and not involved with resistance to hypoxia in lupinus luteus and arabidopsis thaliana during submergence and after recovery of aerobic conditions. we showed a strong nuclear accumulation of poly(a) rna and 6 of 7 studied mrnas with a concurrent strong reduction in rna polymerase ii transcripti ... | 2016 | 27002417 |
| role of asparaginase variable loop at the carboxyl terminal of the alpha subunit in the determination of substrate preference in plants. | structural determinants responsible for the substrate preference of the potassium-independent (aspga1) and -dependent (aspgb1) asparaginases from arabidopsis thaliana have been investigated. like aspga1, aspgb1 was found to be catalytically active with both l: -asn and β-asp-his as substrates, contrary to a previous report. however, aspgb1 had a 45-fold higher specific activity with asn as substrate than aspga1. a divergent sequence between the two enzymes forms a variable loop at the c-terminal ... | 2012 | 22127737 |
| lupin nad9 and nad6 genes and their expression: 5' termini of the nad9 gene transcripts differentiate lupin species. | the mitochondrial nad9 and nad6 genes were analyzed in four lupin species: lupinus luteus, lupinus angustifolius, lupinus albus and lupinus mutabilis. the nucleotide sequence of these genes confirmed their high conservation, however, higher number of nucleotide substitution was observed in the l. albus genes. southern hybridizations confirmed the presence of single copy number of these genes in l. luteus, l. albus and l. angustifolius. the expression of nad9 and nad6 genes was analyzed by northe ... | 2003 | 14557072 |
| use of plant growth promoting bacterial strains to improve cytisus striatus and lupinus luteus development for potential application in phytoremediation. | plant growth promoting (pgp) bacterial strains possess different mechanisms to improve plant development under common environmental stresses, and are therefore often used as inoculants in soil phytoremediation processes. the aims of the present work were to study the effects of a collection of plant growth promoting bacterial strains on plant development, antioxidant enzyme activities and nutritional status of cytisus striatus and/or lupinus luteus plants a) growing in perlite under non-stress c ... | 2017 | 28069305 |