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farnesyl pyrophosphate synthase from white lupin: molecular cloning, expression, and purification of the expressed protein.plants produce a variety of sesquiterpenoid compounds with diverse biological functions, whose synthesis is initiated by farnesyl pyrophosphate synthase [ec 2.5.1.1, ec 2.5.1.10]. the lack of availability of molecular tools to analyze this enzyme has, thus far, prevented the study of its expression and regulation in plants. a dna fragment corresponding to a portion of the farnesyl pyrophosphate synthase gene was amplified by the polymerase chain reaction using was amplified by the polymerase cha ...19957646076
[topography of the metabolic cycle of 4-aminobutyrate].this work describes, with some detail the intervention of 4-aminobutyrate as protagonist of a derivation of tricarboxylic cycle. its vicarial mission is emphasized in connection with its existence in microorganisms (escherichia coli and pseudomonas fluorescens), plants (helianthus tuberosus. lupinus albus and agave americana), neoplasic cells (ascitic tumor of ehrlich and hela cells) and animal tissues (adrenal medulla and brain.19826815735
chemical and nutritional evaluation of sweet lupines.sweet lupines are presently being cultivated at the experimental station in gorbea (chile). four cultivars, lupinus albus cv astra, l. albus cv multolupa, lupinus luteus cv aurea and lupinus angustifolius, were examined for the chemical composition of whole and dehulled seeds and testa. whole seeds contain 34-35% protein. the varieties of l. albus contain 13% oil. l. luteus and l. angustifolius contain about 5% ether extract. crude fiber ranged from 11 to 17% in the whole seed. unsaturated fatty ...19836651228
lupinus albus l. pathogenesis-related proteins that show similarity to pr-10 proteins.we describe a group of three acidic proteins, pathogenesis-related (pr)-p16.5a, pr-p16.5b, and pr-p16.5c, that accumulate in the leaves of lupinus albus l. cv rio maior plants when infected with the fungus colletotrichum gloeosporioides penz. these proteins co-migrate in sodium dodecyl sulfate-polyacrylamide gels as a single band of 16.5 kd, behaving as charge isomers, and are related to several members of the defense-related pr-10 protein family. localization of the proteins was investigated by ...19958539294
effects of white lupine in diets of growing turkeys.in experiment 1, dehulling white lupine (wl) improved growth and feed efficiency of male large white turkey poults fed 40% wl during 4 to 21 days of age. in experiment 2, wl was included proportionally with soybean meal in corn-soy diets of female turkeys from 0 to 17 wk of age. soybean meal: wl ratios (s:wl) were 1:0 (control), 2:1, 1:1, and 6:1. at 17 wk of age hens on s:wl diets of 2:1 had body weights that were similar to those of birds fed the corn-soy control diets. growth of hens fed s:wl ...19883405936
properties of a beta-(1-4)-glucan hydrolase from aspergillus niger.1. a beta-(1-->4)-glucan hydrolase prepared from aspergillus niger, as described by clarke & stone (1965a), showed a ph optimum in the range 4.5-6 and k(m) 0.25% when acting on a cellulose dextrin sulphate substrate. 2. the hydrolase rapidly decreased the specific viscosity of carboxymethylcellulose with a small increase in the production of reducing sugars. the identity of the products of hydrolysis of cellotetraose, cellopentaose and their reduced analogues indicate a preferential cleavage of ...19655862418
[effectiveness of symbiotic n2-fixation in leguminous plants, as affected by inoculation with rhizobia, by substrate, n-fertilizing, and 14c-sucrose application (author's transl)].cultivation experiments (mitscherlich-vessels, quartz sand, 15n-labelled soil, 15n-fertilizer) showed, that various strains of rhizobium lupini (white and yellow lupines) and of rhizobium leguminosarum (field beans and peas) induced a different n2-fixation of the inoculated plants, the most effective rhizobium strains being 367a, cz, t3, 271 (rh. lupini), and azotogen (rh, leguminosarum). yellow lupines and field bean plants were supplied with n2 from the air considerably better than white lupin ...19806252716
digestion of polysaccharides, protein and lipids by adult cockerels fed on diets containing a pectic cell-wall material from white lupin (lupinus albus l.) cotyledon.1. the cell-wall material of white lupin (lupinus albus l.) cotyledon is characterized by low contents of cellulose (47 g/kg) and lignin (17 g/kg) and a high content of pectic substances (710 g/kg). the digestion of lupin cell-wall material by adult cockerels was estimated using gas-liquid chromatographic analyses of alditol acetates derived from polysaccharide sugars. the analyses were performed in the destarched water-insoluble fractions of feed and excreta. digestibility measurements were car ...19852825761
nutritional quality of lupine (lupinus albus cv. multolupa) as affected by lactic acid fermentation.the effects of selected nrrl strains of lactobacillus acidophilus, l. buchneri, l. cellobiosus and l. fermentum upon oligosaccharide, phytate and alkaloid contents, as well as on the nutritive value of lupine, were investigated. lupine was processed to a 12% total solids suspension, inoculated with 1% (v/v) cultures and fermented until a final desired ph of 4.5. l. acidophilus b-2092 and l. buchneri b-1837 growth was related to a significant sucrose breakdown and decreases of phytates, whereas l ...19911790104
lupin as a protein supplement for growing lambs.whole lupins (lupinus albus) were roasted with exit temperatures ranging from approximately 130 (moderate heat) to 175 degrees c (high heat). in situ n disappearance after 12 h of incubation in the rumen was 83% for raw lupins, 45% for lupins roasted at moderate temperatures, and 39% for lupins roasted at high temperatures. lambs fed lupins roasted at moderate temperatures retained more n (p less than .01) than those fed soybean meal (sbm). however, growth rate and feed efficiency were similar a ...19911894575
in situ evaluation of the ruminal and intestinal degradability of extruded whole lupin seed nitrogen.the effect of whole lupin seeds (lupinus albus cv lublanc) at 120, 150 and 195 degrees c on in situ nitrogen degradability (dg.n) was measured by the nylon bag technique using fistulated non-lactating holstein cows. the n degradation was evaluated in nylon bags suspended in the rumen; heating the seeds at 120, 150 and 195 degrees c decreased the dg.n value: 83.9, 72.9 and 53.0 respectively vs 95.3% (rumen outflow rate of 0.06/h). to estimate the total n disappearing in the digestive tract, bags ...19911768316
[lupine, a contribution to the human food supply. 3. nutritional physiological study with lupine (lupinus albus) flour].lupinus albus flour doses of 58.9 +/- 9.6 g have been given to 20 persons. this sweet lupine flour has been digestible without complications in all cases. there have been no significant changes of hemoglobine, hematocrite, total protein, urea, bilirubine, and sgot in the blood. so the lupine may be used for improvement of protein supply in men under the criteria that (1) the alkaloid content of the seed does not exceed 0.02%, (2) the seed itself contains no secondary fungi which may cause a lupi ...19761032959
[nutritive value of lupine and its potential as human food].the chemical composition and some indices of protein quality were measured in two species of sweet lupine lupinus albus and lupinus luteus grown at the experimental station in gorbea, chile: both samples showed a high protein content (39.5 and 44.6%). the protein efficiency ratio (per) was measured in the rat and found to be 0.48 and 0.71, respectively, as compared to 2.57 for casein. supplementation with 0.3% dl-methionine increased significantly those values, thus indicating that lupine protei ...1979550741
[lupine seed meal (lupinus albus cult. astra) as a protein source in swine rations for growth and fattening]. 1979116448
binding of quinolizidine alkaloids to nicotinic and muscarinic acetylcholine receptors.fourteen quinolizidine alkaloids, isolated from lupinus albus, l. mutabilis, and anagyris foetida, were analyzed for their affinity for nicotinic and/or muscarinic acetylcholine receptors. of the compounds tested, the alpha-pyridones, n-methylcytisine and cytisine, showed the highest affinities at the nicotinic receptor, while several quinolizidine alkaloid types were especially active at the muscarinic receptor.19947798968
effect of some environmental factors on rhizobium and bradyrhizobium strains.effects of different abiotic factors (acidity, salinity, nitrate and temperature) on growth rate of root-nodule bacteria (rhizobium and bradyrhizobium) strains were investigated in vitro. strains isolated from vicia faba l., coronilla varia l. and lupinus albus l. exhibited a large variation in tolerance of the above-mentioned factors. these bacteria should be screened under stimulated conditions for enhanced survival before selection to be used for commercial inoculant production. linear correl ...19957620814
immunotropic activity of lupin seeds extracts and fractions from lupinus angustifolius and lupinus albus.the results demonstrated immunotropic activity of seeds extracts and fractions from lupinus angustifolius and lupinus albus. plaque forming cells (pfc) number to srbc (sheep red blood cells) were elevated by an extract from lupinus angustifolius and lowered by extracts from lupinus albus. all preparations obtained from lupinus angustifolius reduced the number of rosette forming cells (e-rfc). these preparations suppressed also the intensity of graft versus host reaction (gvhr) in case when the d ...19947503649
chemical composition, nutritive value, and toxicological evaluation of two species of sweet lupine (lupinus albus and lupinus luteus). 19807391375
the toxicity of seed extracts and their fractions from lupinus angustifolius l. and lupinus albus l.seed extracts obtained from lupinus albus and lupinus angustifolius by treatment with 48% ethanol contained ca. 10% alkaloids (on a dry weight basis) and were non-toxic. their acute toxicity (ld50) in the mouse is > 4000 mg kg-1 body wt. after fractionation, the extract from l. angustifolius seeds afforded several fractions with differing toxicities (ld50 750-4000 mg kg-1 body wt.). none of the fractions tested in vitro were toxic. the results obtained showed that, in spite of the alkaloids, oth ...19938258632
safety evaluation of sweet lupine (lupinus albus cv. multolupa). ii. nine-month feeding and multigeneration study in rats.sweet lupine lupinus albus cv. multolupa) flour containing 41.7% protein and 0.025% lupanine was fed for 9 months to two generations of rats (f1 and f2) at a level that provided 20% dietary protein. the diets were supplemented with 0.2% dl-methionine. the control diet provided the same dietary protein level from defatted soya-bean flour, fish meal and dried skimmed milk. the lupine diet had no effect on the general condition, mortality or behaviour of the animals. the growth rate of males fed sw ...19846537936
purification and characterization of a trna nucleotidyltransferase from lupinus albus and functional complementation of a yeast mutation by corresponding cdna.atp (ctp):trna nucleotidyltransferase (ec 2.7.7.25) was purified to apparent homogeneity from a crude extract of lupinus albus seeds. purification was accomplished using a multistep protocol including ammonium sulfate fractionation and chromatography on anion-exchange, hydroxylapatite and affinity columns. the lupin enzyme exhibited a ph optimum and salt and ion requirements that were similar to those of trna nucleotidyltransferases from other sources. oligonucleotides, based on partial amino ac ...19968616252
white lupin as a protein source for layers.three feeding experiments were conducted with single comb white leghorn hens to determine the effects of lupinus albus cv. ultra on performance. raw, autoclaved, and extruded lupins were fed at 10 and 20% of the diet for 12 wk in experiment 1. in experiment 2 raw and extruded lupins were fed at 10, 15, 20, and 25% of the diet for 16 wk. raw lupin was fed for 32 wk at 10, 15, 20, 25, and 30% of the diet in experiment 3. average egg weight, egg production (percent hen-day basis), and feed consumpt ...19873447138
nutritional value of lupin (lupinus albus)-seed meal for growing pigs: availability of lysine, effect of autoclaving and net energy content.1. two experiments were conducted to assess the nutritional value of lupin (lupinus albus)-seed meal for growing pigs. in the first, the availability of lysine was assessed using slope-ratio analysis. in the second, the effects of autoclaving lupin seeds and formulating the diets on the basis of estimated digestible or net energy were assessed. 2. in the first experiment, the availability of lysine in three samples of lupin-seed meal was compared with that in meat-and-bone meal and soya-bean mea ...19863118937
[ruminal digestion and intestinal absorption of lupine proteins extruded in the lactating cow].four lactating cows fitted with permanent ruminal, duodenal and ileal cannulae were used to study the effect of extrusion of whole lupin seeds at 195 degrees c (lupinus albus cv lublanc) on organic matter (om) and nitrogen (n) degradation in the rumen and their flow to and absorption from the small intestine. raw whole lupin seeds (rwls) and extruded whole lupin seeds (ewls) were fed in diets containing 15.5% crude protein and composed of 22.6% whole lupin seeds, 56.5% corn silage, 10.2% corn gr ...19911777057
[lupine, a contribution to the human food supply. 4. acceptability of lupinus alba flour].48 different dishes enriched with lupinus albus flour have been tested by 20 persons. the test duration has been 4 weeks. meals and products with lupine admixtures have been judged equally with concern to corresponding conventional dishes. farinaceous products had the best test results. therefore, it is recommended for peru to add lupine flour when producing bread, biscuits, sauces, soups, and noodles (maccaronis etc.). the test time had no influence to the test results.19761020375
characterisation of a developmentally related polypeptide with glutelin solubility characteristics from lupinus albus l.proteins from lupinus albus l. cv. rio maior seeds were fractionated according to solubility criteria. patterns of concanavalin a (cona)-binding polypeptides from the different classes, albumins, globulins, glutelins and prolamins, were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. two bands of apparent molecular masses of 29 and 23.5 kda with glutelin solubility characteristics bound the lectin. the 23.5-kda band was separated by two-dimensional electrophoresis into ...19968580776
evaluation of white lupines and triticale in calf starter diets.one hundred fifty-two holstein calves were used to evaluate white lupines (lupinus albus cv. ultra) and triticale in starter diets. the basal diet contained corn, oats, minerals, and vitamins. starter components were 1) 14% soybean meal; 2) 10% soybean meal and 27% triticale; 3) 22% lupines; 4) 16% lupines and 24.5% triticale; 5) 11% lupines and 8% soybean meal; and 6) 7% lupines, 5% soybean meal, and 26.5% triticale. dietary cp was approximately 16% of dm. data collection began on d 4 after bir ...19892745803
[the lupine, a contribution to nutrition in the andes. 2. the quality of proteins in lupinus mutabilis compared to lupinus albus, lupinus luteus and soja max]. 19751224691
effect of food enzymes on utilisation of lupin carbohydrates by broilers.1. the effects of 3 commercial enzyme products on the nutritive value of 2 lupin species were investigated with the emphasis on changes in composition of non-starch polysaccharides (nsps) along the digestive tract. enzyme a contained primarily cellulase, beta-glucanase and xylanase activities, enzyme b primarily hemicellulase, pentosanase and xylanase activities, and enzyme c primarily hemicellulase, pectinase and beta-glucanase activities. 2. the enzymes were added to semi-purified diets based ...200010821527
direct evidence for ribonucleolytic activity of a pr-10-like protein from white lupin roots.an abundant 17 kda protein which was isolated and characterized from 10-day old healthy root tissue of white lupin (lupinus albus) proved to have a high sequence similarity to pathogenesis-related proteins found in other species. subsequently, a corresponding clone (lapr-10) was identified in a cdna library prepared from the same tissue that exhibited a high amino acid sequence similarity to a number of the pr-10 family proteins. the clone contains an open reading frame encoding a polypeptide of ...200010890534
genotypic and environmental effects on lupin seed composition.currently, white lupin (lupinus albus l.) is gaining importance due to its high nitrogen fixation capability and potential in sustainable crop production systems. even though research conducted in australia, chile, germany, new zealand, and portugal has indicated lupin's positive potential as human and animal food, such information from virginia and adjoining areas of the united states is not available. in addition, effects of growing environment and genotypes on lupin seed composition need to b ...199810890753
a new class of biflavonoids: 2'-hydroxygenistein dimers from the roots of white lupin.two novel isoflavonoid dimers presumably originating from 2'-hydroxygenistein, 5,7,4'-trihydroxycoumaranochroman-4-one-(3-->5"')-5",7",2"'4"'- tetrahydroxyisoflavone (1, lupinalbisone a) and 5,7,4'-trihydroxycoumaranochroman-4-one-(3-6")-5",7",2"',4"'-te trahydroxyisoflavone (2, lupinalbisone b) were isolated from the roots of lupinus albus l., and their structures involving relative stereochemistry were elucidated by spectroscopic methods. using horse radish peroxidase and 2'-hydroxygenistein ( ...200010817204
effect of enzyme supplementation on the nutritional value of raw, autoclaved, and dehulled lupins (lupinus albus) in chicken diets.the effects of adding crude enzyme preparations to diets containing raw, autoclaved, and dehulled lupin seeds on the performance of broiler and leghorn chicks (7 to 21 days) were evaluated in five experiments. in the first experiment, enzyme addition (combination of energex-carbohydrase, bio-feed pro-protease, and novozyme-alpha-galactosidase) to a diet containing 70% raw lupins improved the weight gain and feed to gain ratio of broiler chicks by 18 and 10%, respectively. the relative intestinal ...19938309877
variability in plant-microbe interaction between lupinus lines and bradyrhizobium strains.even though lupin (lupinus albus l.) is known to potentially fix 150-200 kg/ha nitrogen for the use of a succeeding crop, precise information about lupinxbradyrhizobium strain interaction under the climatic conditions prevalent in the mid-atlantic region of the united states is unknown. we conducted two greenhouse experiments with the objective of characterizing this symbiotic relationship and to evaluate potential interaction between bradyrhizobium strains and lupin lines. in the first experime ...200011074278
differential expression of four genes encoding 1-aminocyclopropane-1-caroboxylate synthase in lupinus albus during germination, and in response to indole-3-acetic acid and wounding.1-aminocyclopropane-1-carboxylate (acc) synthase (acs; ec 4.4.1.14) is the key regulatory enzyme of the ethylene biosynthetic pathway and is encoded by a multigene family in arabidopsis thaliana, tomato, mung bean and other plants. southern blot analysis revealed the existence of at least five acs genes in white lupin (lupinus albus l.) genome. four complete and one partial sequences representing different acs genes were cloned from the lupin genomic library. the levels of expression of two of t ...200011089679
profiling changes in metabolism of isoflavonoids and their conjugates in lupinus albus treated with biotic elicitor.liquid chromatography with ultraviolet and mass spectrometric detection was applied to monitor changes in profiles of isoflavonoid glycosides and free isoflavonoid aglycones in lupinus albus l. four isoflavonoid aglycones, fourteen isoflavonoid glycosides, four flavonol glycosides and flavone glycoside were identified in lupin tissue after lc/esi/ms analyses. an elicitor preparation from purified yeast cell wall was used to inject the shoots of 3-week old seedlings or to infiltrate the cut lupin ...200111198822
root colonization of different plants by plant-growth-promoting rhizobium leguminosarum bv. trifolii r39 studied with monospecific polyclonal antisera.monospecific polyclonal antisera raised against rhizobium leguminosarum bv. trifolii r39, a bacterium which was isolated originally from red clover nodules, were used to study the colonization of roots of leguminous and nonleguminous plants (pisum sativum, lupinus albus, triticúm aestivum, and zea mays) after inoculation. eight weeks after inoculation of soil-grown plants, between 0.1 and 1% of the total bacterial population in the rhizospheres of all inoculated plants were identified as r. legu ...19979143133
characterization and expression analysis of the yellow lupin (lupinus luteus l.) gene coding for nodule specific proline-rich protein.the llprp2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of enod2 nodule-specific genes. several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the llprp2 gene. northern blot analysis revealed that the expression of the llprp2 gene begins 9 days after inoculation of yellow lupin roots with bradyrhizobium sp. (lupinus); the expression is restricted to symbiotic ...200011051202
effect of sweet, bitter and soaked micronised bitter lupins on broiler performance.1. the feeding value for broilers of sweet white lupins (lupinus albus variety hanti), bitter lupins (lupinus angustifolius) and soaked micronised bitter lupins was examined. 2. four isocaloric and isonitrogenous diets were formulated; one contained no lupins and the other 3 contained 400 g/kg sweet, bitter or soaked micronised bitter lupins. the 3 lupin diets were blended appropriately to produce 16 experimental diets containing 0, 50, 100, 200, 300 and 400 g/kg sweet, bitter and soaked microni ...19979158898
comparative pharmacological study of sparteine and its ketonic derivative lupanine from seeds of lupinus albus. 19846151710
pathogenic infection and the oxidative defences in plant apoplast.the structural and functional continuum of the plant apoplast is the first site of contact with a pathogen and plays a crucial role in initiation and coordination of many defence responses. in this paper, we present an overview of the involvement of the plant apoplast in plant-pathogen interactions. the process of infection of french bean (phaseolus vulgaris l.) plants by colletotrichum lindemuthianum is analysed. the ultrastructural features of plant defence responses to fungal infection are th ...200111732333
analysis of lupin seed protein digestibility using gel electrophoresis and immunoblots.proteins from the seeds of 12 cultivars of three lupin species were analyzed by gel electrophoresis. similarities between cultivars of the same species were noted. antibodies raised against the three major globular proteins, conglutin alpha, beta, and gamma, of lupinus albus cv. ultra were used to probe immunoblots of crude extracts. the immunoblots revealed variations between cultivars not previously resolved and identified which protein-subunits were derived from which conglutin. in vitro dige ...19979222852
effect of enzyme supplementation of uk-grown lupinus albus on growth performance in broiler chickens. 199810188036
lupins (lupinus albus) as a protein supplement for lactating holstein dairy cows.three experiments were conducted to determine optimal substitution of lupin for soybean meal in dairy cattle diets; the effects of whole versus ground lupins and the effects of whole lupins versus whole soybeans on milk production were examined. in the first experiment, 57 cows were assigned randomly to treatments in blocks of five by calving order within parity. treatments began 22 d postpartum and continued through d 140. diets were isonitrogenously balanced using soybean meal as control: lupi ...19938227670
traditional antihelmintic, antiparasitic and repellent uses of plants in central italy.the uses of 51 plants of marche, abruzzo and latium, distributed in 28 families, are listed here. memories and news of continued use of the plants in these sectors were collected from farmers and shepherds in person (mostly old people). the plants most frequently used as antiparasitics and repellents are juglans regia, lupinus albus, ruta graveolens, fraxinus ornus, datura stramonium, artemisia absinthium, allium cepa, while r. graveolens, cucurbita maxima, a. absinthium, allium sativum are the ...199910624877
inhibition of growth and aflatoxin biosynthesis of aspergillus flavus by extracts of some egyptian plants.the effect of five different concentrations (2, 4, 6, 8 and 10 mg ml-1) of an aqueous extracts of lupinus albus, ammi visnaga and xanthium pungens were tested on growth and aflatoxin production by aspergillus flavus in a chemically defined medium. all the plants inhibited mycelial growth and aflatoxin formation. the inhibitory effect was proportional with the applied concentration. growth and aflatoxin production appeared to be correlated processes. the nature of the plant extract also affected ...199910664975
[risk of serious acute asthma due to lupine flour associated with peanut allergy].lupine flour (lupinus albus), recently authorized in france in human food, cross-reacts with peanuts. we report a case of acute asthma in a patient with allergy to peanuts. exegesis: this patient has a severe allergy to peanuts, presenting as acute asthma. skin prick-tests to raw and cooked lupine flour were positive. the level of specific-ige (allerbio, france) to lupine flour were high. oral challenge test induced acute asthma at a dose of 965 mg of lupine flour. this quantity may be included ...200010703077
accumulation of a lectin-like breakdown product of beta-conglutin catabolism in cotyledons of germinating lupinus albus l. seeds.during germination of lupinus albus seeds, a 20-kda polypeptide accumulates in the cotyledons of 4-d-old plants (ferreira et al., 1995b, j exp bot 46: 211-219). immunological, polypeptide cleavage with cyanogen bromide and amino acid sequencing experiments indicate that the 20-k-da polypeptide and ubiquitin are structurally unrelated. however, there is a strong sequence homology between the 20-kda polypeptide and the vicilin-like storage proteins from pea and soybean. our results indicate that t ...19979299789
the lupinus albus class-iii chitinase gene, if3, is constitutively expressed in vegetative organs and developing seeds.a cdna fragment encoding a lupinus albus. l. class-iii chitinase, if3, was isolated, using a cdna probe from cucumis sativus l., by in-situ plaque hybridization from a cdna library constructed in the uni-zap xr vector, with mrnas isolated from mature lupin leaves. the cdna had a coding sequence of 293 amino acids including a 27-residue n-terminal signal peptide. a class-iii chitinase gene was detected by southern analysis in the l. albus genome. western blotting experiments showed that the if3 p ...200010787047
a calluna vulgaris extract 5-lipoxygenase inhibitor shows potent antiproliferative effects on human leukemia hl-60 cells.a water-calluna vulgaris extract (water-cve) was found to be a relatively specific arachidonate 5-lipoxygenase inhibitor and showed potent anti-proliferative effects on human leukemic hl60 cells. water-cve completely inhibited potato 5-lipoxygenase activity at 250 micrograms/ml, partially inhibited soybean 15-lipoxygenase at ph 7.4 and had no effect either on this 15-lipoxygenase at its optimal activity ph (ph 9) or on lupinus albus 5-, 8-, 15-lipoxygenase. in culture, the proliferation and dna ...19921419080
effects of a vegetable extract from lupinus albus (lu105) on the production of matrix metalloproteinases (mmp1, mmp2, mmp9) and tissue inhibitor of metalloproteinases (timp1, timp2) by human gingival fibroblasts in culture.this study examined the effects of a vegetable extract from lupinus albus (lu105) on mmps and timps secreted by human gingival fibroblasts in culture. lu105 was extracted from seeds of l. albus and is freely soluble in water. gelatin zymography showed that control human gingival fibroblasts maintained in culture for 48 h express pro-mmp2 (progelatinase a) in the culture medium while the active form of mmp2 (gelatinase a), the active form of mmp9 (gelatinase b), and pro-mmp9 (progelatinase b) are ...200312802622
profiling of flavonoid conjugates in lupinus albus and lupinus angustifolius responding to biotic and abiotic stimuli.qualitative and quantitative composition of flavonoid and isoflavonoid glycosides as well as free aglycones in lupin seedlings (roots and aerial parts) grown under different light conditions or responding to infection with pleiochaeta setosa, a fungus causing brown leaf spot, were monitored by liquid chromatography with uv and/or mass spectrometric detection. both physical and biotic factors affected flavonoid and isoflavonoid levels in lupin tissues. fungal infection evoked significant increase ...200312857026
biochemical study on the effects of some egyptian herbs in alloxan-induced diabetic rats.the present study was carried out to investigate the effects of lupinus albus, l. (lupinus termis), family l. leguminosae, cymbopogon proximus, (halfa barr), family gramineae, and zygophyllum coccineum l. (kammun quaramany), family l. zygophyllacae on biochemical parameters in alloxan-induced diabetic rats. a dose of 1.5 ml of aqueous suspension of each herb/100 g body weight (equivalent to 75 mg/100 g b.wt.) was orally administered daily to alloxan-diabetic rats for 4 weeks. the levels of gluco ...200211788159
chemical composition and antibacterial activities of lupin seeds extracts.determination of influence of lupin natural phenolic compounds on antibacterial properties of its seeds was carried out. raw material were seeds of lupinus albus, l. luteus, and l. angustifolius. the methods included the determination of the content of proteins, total phenolic compounds, free phenolic acids, and tannins as well as antibacterial properties with ethanol extracts. the content of total phenolic compounds was smaller in testas than in cotyledons and the highest levels are observed in ...200314609081
characterization of isoperoxidase-b2 inactivation in etiolated lupinus albus hypocotyls.one basic peroxidase isoenzyme, with a pi of 8.8, is present in the intercellular washing fluid in the aerial part of 6-day-old lupinus albus hypocotyl seedlings. this isoenzyme, called lup-b2, is the principal soluble component secreted into the apoplastic space and it is a constitutive enzyme along the whole length of etiolated hypocotyl. the enzymatic inactivation process which this apoplastic peroxidase undergoes is described for the first time. the kinetic constants which describe its inact ...200010719177
the bioavailability and postprandial utilisation of sweet lupin (lupinus albus)-flour protein is similar to that of purified soyabean protein in human subjects: a study using intrinsically 15n-labelled proteins.sweet lupin (lupinus albus), a protein-rich legume devoid of anti-nutritional factors, is considered to have a high potential for protein nutrition in man. results concerning the nutritional value of lupin protein are, however, conflicting in animals and very scarce in human subjects. furthermore, where fibre-rich protein sources are concerned, the long-term nutritional results are often obscured, particularly since fibre-promoted colonic fermentation may bias the energy supply and redistribute ...200212064341
the effect of nitrogen nutrition on cluster root formation and proton extrusion by lupinus albus.nitrogen nutrition can influence cluster root formation in many wild species, but the effect of n form on cluster root formation and root exudation by white lupin is not known. in a solution culture study, we examined the effect of n nutrition (ammonium, nitrate, both or n2 fixation) on cluster root formation and h+ extrusion by white lupin plants under deficient and adequate p supply. the number of cluster roots increased greatly when plants were supplied with i microm p compared with 50 microm ...200212096804
purification and characterization of three phytases from germinated lupine seeds (lupinus albus var. amiga).three phytases were purified about 14200-fold (lp11), 16000-fold (lp12), and 13100-fold (lp2) from germinated 4-day-old lupine seedlings to apparent homogeneity with recoveries of 13% (lp11), 8% (lp12), and 9% (lp2) referred to the phytase activity in the crude extract. they behave as monomeric proteins of a molecular mass of about 57 kda (lp11 and lp12) and 64 kda (lp2), respectively. the purified proteins belong to the acid phytases. they exhibit a single ph optimum at 5.0. optimal temperature ...200212405788
nutrient supply to dairy cows from processed white lupines.the objective of this study was to compare the dve/oeb system (dve = truly absorbed protein in the small intestine; oeb = degraded protein balance (dpb) in dutch) and the nrc-2001 model in the prediction of supply of protein to dairy cows from processed white lupines (lupinus albus l.). comparisons were made in terms of (1) ruminally synthesized microbial cp, (2) truly absorbed protein in the small intestine, and (3) degraded protein balance. in addition, the systematic investigation of roasting ...200415195906
accumulating behaviour of lupinus albus l. growing in a normal and a decalcified calcic luvisol polluted with zn.lupinus albus l. is a leguminous plant that is starting to generate interest for the phytoremediation soils showing intermediate metal pollution. among these metals, zn causes major phytotoxicity problems and is common in polluted soils of central spain. the purpose of this study was to explore the nutritional behaviour of this plant species towards increasing zn concentrations in two calcic luvisol soils: a normal basic soil and a decalcified acid soil. for this purpose the effects of different ...200314717438
ethnobotanical and ethnomedicinal uses of plants in the district of acquapendente (latium, central italy).in the years 2002-2003 research was carried out concerning ethnomedicine in the acquapendente district (viterbo, latium, central italy), an area so far less frequently studied from the perspective of plant folk traditions. the district, from the ethnobotanical point of view, shows traces of the influences of the neighbouring regions. in this study 96 plant entities are described, belonging to 45 families, of which 64 are employed in human medicine, 15 in veterinary medicine, 22 in the feeding of ...200515619562
traditional phytotherapy in central italy (marche, abruzzo, and latium).in this study, the more significant results of extensive ethnopharmacobotanical research carried out by the author in the years 1977-2000 in 175 localities of three regions of central italy (marche, abruzzo, and latium) have been reported and compared. the usages of 80 species belonging to 36 families are described, of which 71 were used in human therapy and 29 in veterinary medicine. uses are suited with the number of localities in which they have been mentioned. among the wild plant mainly sti ...200515664457
nodulation of lupinus albus by strains of ochrobactrum lupini sp. nov.the nodulation of legumes has for more than a century been considered an exclusive capacity of a group of microorganisms commonly known as rhizobia and belonging to the alpha-proteobacteria. however, in the last 3 years four nonrhizobial species, belonging to alpha and beta subclasses of the proteobacteria, have been described as legume-nodulating bacteria. in the present study, two fast-growing strains, lup21 and lup23, were isolated from nodules of lupinus honoratus. the phylogenetic analysis ...200515746334
protective effect of a vegetable extract from lupinus albus (lu 105) on human gingival elastic fibers degradation by human leukocyte elastase.the aim of this study was to determine if a vegetable extract from seeds of lupinus albus (lu 105) has the capacity to inhibit human leukocyte elastase and/or protect gingival elastic fibers against proteolytic degradation. lu105 was extracted from seeds of l albus and is freely soluble in water. in this study the ex-vivo elastolytic activity of human leukocyte elastase and the potential inhibitory effect of lu 105 were determined using human gingival cryostat tissue sections and computerized mo ...200314508666
raffinose family of oligosaccharides from lupin seeds as prebiotics: application in dairy products.the raffinose family of oligosaccharides (rfos) isolated from lupin seeds (lupinus albus var. multolupa) was evaluated for bifidogenic effects during the manufacture of probiotic fermented milk. a mixed starter inoculum was composed of bifidobacterium lactis bb-12 and lactobacillus acidophilus (1:1). lupins are a rich source of rfos that can be used as functional food ingredients. the addition of rfos to milk increased b. lactis bb-12 and l. acidophilus populations at the final fermentation time ...200515954717
characterization of bacterial community structure in rhizosphere soil of grain legumes.molecular techniques were used to characterize bacterial community structure, diversity (16s rdna), and activity (16s rrna) in rhizospheres of three grain legumes: faba beans (vicia faba l., cv. scirocco), peas (pisum sativum l., cv. duel) and white lupin (lupinus albus l., cv. amiga). all plants were grown in the same soil under controlled conditions in a greenhouse and sampled after fruiting. amplified 16s rdna and rrna products (using universal bacterial primers) were resolved by denaturing g ...200516003473
proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase ldl receptor activity in hepg2 cells.white lupin (lupinus albus, l.), a widely cultivated crop that has been consumed for many years in western europe, may provide a useful alternative for individuals wishing to substitute animal with plant proteins for cardiovascular disease prevention. lupin seeds have a very low content of isoflavones, and lupin protein isolates are essentially isoflavone free. in rats fed a casein-based cholesterol + cholic acid diet, a relatively low daily intake (50 mg/d by gavage for 2 wk) of total lupin pro ...200414704287
soil to plant transfer of 238u, 226ra and 232th on a uranium mining-impacted soil from southeastern china.both soil and plant samples of nine different plant species grown in soils from southeastern china contaminated with uranium mine tailings were analyzed for the plant uptake and translocation of 238u, 226ra and 232th. substantial differences were observed in the soil-plant transfer factor (tf) among these radionuclides and plant species. lupine (lupinus albus) exhibited the highest uptake of 238u (tf value of 3.7x10(-2)), while chinese mustard (brassica chinensis) had the least (0.5x10(-2)). how ...200515878419
genetic structure and symbiotic characteristics of a bradyrhizobium population recovered from a pasture soil.we examined the genetic structure and symbiotic characteristics of bradyrhizobium isolates recovered from four legume species (lupinus albus [white lupine], lupinus angustifolius [blue lupine], ornithopus compressus [yellow serradella], and macroptilium atropurpureum [sirato]) grown in an oregon soil. we established that multilocus enzyme electrophoresis (mlee) can provide insights into the genetic relatedness among bradyrhizobium strains by showing a positive correlation (r = >/=0.90) between t ...199416349270
plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of lupin analysed by phytate utilization ability.in the rhizosphere, phosphorus (p) levels are low because of p uptake into the roots. rhizobacteria live on carbon (c) exuded from roots, and may contribute to plant nutrition by liberating p from organic compounds such as phytates. we isolated over 300 phytate (na-inositol hexa-phosphate; na-ihp)-utilizing bacterial strains from the rhizosheath and the rhizoplane of lupinus albus (l.). almost all of the isolates were classified as burkholderia based on 16s rdna sequence analysis. rhizosheath is ...200515683400
nutritional evaluation of protein, phosphorus, calcium and magnesium bioavailability from lupin (lupinus albus var. multolupa)-based diets in growing rats: effect of alpha-galactoside oligosaccharide extraction and phytase supplementation.the nutritional composition of the legume lupinus albus var. multolupa, raw or after alpha-galactoside extraction, and its effect on the bioavailability of protein, p, ca, and mg by growing rats was evaluated using a balance technique. the protein and dietary fibre content of the lupin flours studied was high, and 89-94% of the dietary fibre was present as insoluble dietary fibre. the alpha-galactoside extraction process did not disrupt the nutritional quality of protein, and the digestive and m ...200616768832
effect of some hypoglycemic herbs on the activity of phase i and ii drug-metabolizing enzymes in alloxan-induced diabetic rats.polycyclic aromatic hydrocarbons (pahs) and n-nitrosamines (nna) are mainly activated by cytochrome p450s, and their associated enzyme activities such as aryl hydrocarbon (benzo(a)pyrene) hydroxylase (ahh), n-nitrosdimethylamine n-demethylase i (ndma-di), nadph-cytochrome c reductase, and detoxified by glutathione s-transferase (gst) and glutathione (gsh). the present study shows the influence of cymbopogon proximus (halfa barr), zygophyllum coccineum l. (kammun quaramany), lupinus albus (termis ...200211985890
involvement of mitogen-activated protein kinases in the symbiosis bradyrhizobium-lupinus.in plants, mitogen-activated protein kinases (mapks) are involved in signalling to hormones, cell cycle regulation, stresses, and plant defence responses. in this work, several mapks were detected by immunobloting in roots and nodules of lupinus albus produced by inoculation with bradyrhizobium sp. (lupinus). in vitro kinase assays showed that inoculation of seedling roots with b. sp. (lupinus) activates salt stress-inducible and stress-activated mapks after 5 min of incubation. by contrast, ino ...200616868044
lupinus albus, a novel vegetable extract with metalloproteinase inhibitory properties: a potential periodontal therapy.in this study we examine the properties of a vegetable extract from seeds of lupinus albus (lu 105). in previous works we demonstrated that lu 105 reduced the expression, by gingival fibroblasts, of both matrix metalloproteinase (mmp)-2 and mmp-9. we decided to study the impact of lu 105 on cell proliferation and morphology. using organ culture media we also studied the mmp and tissue inhibitors of metalloproteinases (timp) expression and the cytokines secretion.200516101366
monoclonal antibodies against the candidate lupin allergens alpha-conglutin and beta-conglutin.the ingestion of dietary products containing sweet lupin (such as lupinus albus or lupinus angustifolius) has been reported to cause ige-mediated allergic reactions. recent studies have indicated lupin globulins as important ige binding proteins. the aim of the present study was to generate and characterize monoclonal antibodies (mabs) against lupin seed proteins.200717192716
effects of extracts of lupine seed on blood glucose levels in glucose resistant mice: antihyperglycemic effects of lupinus albus (white lupine, egypt) and lupinus caudatus (tailcup lupine, mesa verde national park).lupine is a medicinal food plant with potential value in the management of diabetes. in white mice, extracts of seeds of the white lupine [lupinus albus (l. termis l.)] were associated with increased tolerance to an oral glucose bolus. antihyperglycemic activity was present in extracts of the whole seed but not extracts of the seed coat, and was not detected when glucose was administered intraperitoneally rather than orally. however, in contrast to results seen with the prescription drug, acarbo ...200617317651
phyllobacterium trifolii sp. nov., nodulating trifolium and lupinus in spanish soils.bacterial strain petp02(t) was isolated from nodules of trifolium pratense growing in a spanish soil. phylogenetic analysis of the 16s rrna gene sequence showed that this strain represents a member of the genus phyllobacterium. however, divergence found with the 16s rrna gene sequence of the single recognized species of this genus, phyllobacterium myrsinacearum, indicated that strain petp02(t) belongs to a different species. the results of dna-dna hybridization, phenotypic tests and fatty acid a ...200516166699
assessment of lupin allergenicity in the cholera toxin model: induction of ige response depends on the intrinsic properties of the conglutins and matrix effects.the well-established murine model of ige-mediated food allergy, based on oral administration of antigen and cholera toxin (ct), has within the previous years been used to evaluate various food proteins. nonetheless, little knowledge on the factors that determine the allergenicity of food proteins is available so far. the use of proteins from the legume seed lupinus albus as food ingredients calls for an evaluation of their allergenic potential, and therefore, we applied the cited model to invest ...200616864993
lupin protein influences the expression of hepatic genes involved in fatty acid synthesis and triacylglycerol hydrolysis of adult rats.to assess the effect of lupin protein on concentrations of lipids in plasma lipoproteins and liver and hepatic mrna concentrations of genes involved in lipid metabolism, adult rats were fed egg albumin-based diets containing either lupin protein from lupinus albus or casein (50 g/kg) supplemented (hypercholesterolaemic) or not (normolipaemic) with a cholesterol-cholate mixture for 20 d. lupin protein compared with casein lowered the concentrations of tag in liver (p < 0.01) and circulating vldl ...200818096091
dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c.recently, it has been shown that dietary lupin protein lowers plasma triglyceride concentrations in rats. in this study, we investigated the hypothesis that this effect is due to a downregulation of sterol regulatory element-binding protein (srebp)-1c, a transcription factor that regulates the expression of lipogenic enzymes in the livers of rats.200717785965
suppression of phytophthora cinnamomi in potting mixes amended with uncomposted and composted animal manures.abstract we examined the effects of fresh and composted animal manures on the development of root rot, dieback, and plant death caused by phytophthora cinnamomi. fresh chicken manure, or chicken manure composted for 5 weeks before incorporation into the potting mix (25%, vol/vol), significantly reduced pathogen survival and the development of symptoms on lupinus albus seedlings. chicken manure composted for 2 weeks was less suppressive. cow, sheep, and horse manure, whether fresh or composted, d ...200018944498
transgenic proteoid roots of white lupin: a vehicle for characterizing and silencing root genes involved in adaptation to p stress.white lupin (lupinus albus l.) has become an illuminating model for the study of plant adaptation to phosphorus (p) deficiency. it adapts to -p stress with a highly coordinated modification of root development and biochemistry resulting in short, densely clustered secondary roots called proteoid (or cluster) roots. in order to characterize genes involved in proteoid root formation and function in a homologous system, we have developed an agrobacterium rhizogenes-based transformation system for w ...200516297074
hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model.the biological activities of a protein isolate from lupin (lupinus albus) were studied in a rabbit model of atherosclerosis. focal plaque development was induced at both common carotid arteries by perivascular injury. after surgery, animals were fed three different diets for 90 d, all with 1 % cholesterol, 15 % sfa and 20 % protein; the protein source was casein (cas), lupin proteins (lup) or 50 % cas+50 % lup (cas+lup). lower cholesterolaemia was detected in the lup v. the cas group at 60 and 9 ...200818315889
combined 2d electrophoretic approaches for the study of white lupin mature seed storage proteome.seed proteome analysis by 2d ief/sds-page techniques is challenging for the intrinsic difficulties related to quantitative disparity of the seed proteins, i.e. storage and non-storage proteins, their polymorphic nature, the extensive post-translational modifications and the paucity of deposited primary structures available. conversely, 2d maps of seed proteomes can be extremely useful for a number of fundamental and applied investigations. in this work, we have used a combination of two experime ...200717320919
iron stress and pyoverdin production by a fluorescent pseudomonad in the rhizosphere of white lupine (lupinus albus l.) and barley (hordeum vulgare l.).induction of high-affinity iron transport during root colonization by pseudomonas fluorescens pf-5 (pvd-inaz) was examined in lupine and barley growing in microcosms. p. fluorescens pf-5 (pvd-inaz) contains a plasmid carrying pvd-inaz; thus, in this strain, ice nucleation activity is regulated by pyoverdin production. lupine or barley plants were grown for 18 or 8 days, respectively, in soil amended with 2% calcium carbonate and inoculated with p. fluorescens pf-5 (pvd-inaz) at a density of 4 x ...199716535491
sandwich enzyme-linked immunosorbent assay (elisa) for the detection of lupine residues in foods.lupine has been increasingly used in food applications due to its high nutritional value and excellent functional properties. however, lupine provokes allergic reactions in susceptible individuals. the presence of undeclared lupine residues in foods can pose a serious health risk to lupine-allergic individuals. therefore, the objective of this research was to develop a sandwich-type elisa for the detection of lupine residues in foods. lupine flour derived from lupinus albus was used to immunize ...200819019135
lupin (lupinus albus) protein isolate (l-iso) has adequate nutritional value and reduces large intestinal weight in rats after restricted and ad libitum feeding.a protein isolate from white lupin (lupinus albus; l-iso) has potential as a novel human food ingredient, but its nutritional effects are unknown.200617191026
alterations induced by glyphosate on lupin photosynthetic apparatus and nodule ultrastructure and some oxygen diffusion related proteins.the effects of glyphosate on protein metabolism, mesophyll cell ultrastructure and nodule ultrastructure and functioning of lupinus albus cv. multolupa inoculated with bradyrhizobium sp. (lupinus) were investigated. young leaves and nodules were especially affected because these organs act as sinks of the herbicide. the alterations on nodular and chloroplast ultrastructure varied depending on herbicide concentration and time of exposure. after 3 days of 2.5 mm glyphosate application some toxic e ...200516324849
effects of n(2) deficiency on transport and partitioning of c and n in a nodulated legume.nodulated root systems of white lupin (lupinus albus l. cv ultra: rhizobium strain wu425) were exposed to ar:o(2) (80:20, v/v) or ar:n(2):o(2) (70:10:20, v/v/v) and c and n partitioning were examined over a 9- or 10-day period in comparison with control plants with nodulated roots retained in air. accumulation of n ceased in plants exposed to ar:o(2) or was much reduced in plants exposed to ar:n(2):o(2), but net c assimilation rates and profiles of c utilization remained similar to those of cont ...198416663823
leghemoglobin in lupin plants (lupinus albus cv multolupa).leghemoglobin was localized by immunogold techniques in nodules of lupinus albus cv multolupa inoculated with bradyrhizobium sp. (lupinus) strain islu 16. the protein localization was performed in nodules embedded in spurr's and araldite epoxy resins and lowycryl k4m. a very good preservation of both the ultrastructure and antigenicity was obtained with the tissues embedded in araldite following glutaraldehyde fixation and unpostfixed in osmium tetroxide. lupin leghemoglobin is a stable and abun ...198916666792
effects of short-term n(2) deficiency on n metabolism in legume nodules.the study aimed to test the hypothesis that ammonia production by rhizobium bacteroids provides not only a source of nitrogen for growth but has a central regulatory role in maintaining the metabolic activity and functional integrity of the legume nodule. production of ammonia in intact, attached nodules was interrupted by short-term (up to 3 days) exposure of the nodulated root systems of cowpea (vigna unguiculata l. walp cv vita 3: rhizobium cb 756) and lupin (lupinus albus l. cv ultra: rhizob ...198416663910
the potential of oil-utilizing bacterial consortia associated with legume root nodules for cleaning oily soils.the surfaces of root nodules of vicia faba and lupinus albus (legume crops), were colonized with bacterial consortia which utilized oil and fixed nitrogen. such combined activities apparently make those periphytic consortia efficient contributors to bioremediation of oily nitrogen-poor desert soils. this was confirmed experimentally in this study. thus, cultivating v. faba, l. albus and, for comparison, solanum melongena, a nonlegume crop, separately in oily sand samples resulted in more oil att ...200919103456
a label-free internal standard method for the differential analysis of bioactive lupin proteins using nano hplc-chip coupled with ion trap mass spectrometry.quantitative proteomics based on ms is useful for pointing out the differences in some food proteomes relevant to human nutrition. stable isotope label-free (sif) techniques are suitable for comparing an unlimited number of samples by the use of relatively simple experimental workflows. we have developed an internal standard label-free method based on the intensities of peptide precursor ions from ms/ms spectra, collected in data dependent runs, for the simultaneous qualitative characterization ...200919105171
economy of photosynthate use in nitrogen-fixing legume nodules: observations on two contrasting symbioses.the economy of c use by root nodules was examined in two symbioses, vigna unguiculata (l.) walp. (cv. caloona):rhizobium cb756 and lupinus albus l. (cv. ultra):rhizobium wu425 over a 2-week period in early vegetative growth. plants were grown in minus n water culture with cuvettes attached to the nodulated zone of their primary roots for collection of evolved co(2) and h(2). increments in total plant n and in c and n of nodules, and c:n weight ratios of xylem and phloem exudates were studied by ...197916661076
occurrence of h(2)-uptake hydrogenases in bradyrhizobium sp. (lupinus) and their expression in nodules of lupinus spp. and ornithopus compressus.fifty-four strains of bradyrhizobium sp. (lupinus) from worldwide collections were screened by a colony hybridization method for the presence of dna sequences homologous to the structural genes of the bradyrhizobium japonicum hydrogenase. twelve strains exhibited strong colony hybridization signals, and subsequent southern blot hybridization experiments showed that they fell into two different groups on the basis of the pattern of ecori fragments containing the homology to the hup probe. all str ...198916666550
nutritive evaluation of legume seeds for ruminant feeding.chemical composition, rumen degradability and the effect of particle losses, and intestinal digestibility of protein by using in situ-in vitro and in vitro techniques were stated for beans (vicia faba), lupin (lupinus albus), vetch (vicia sativa) and bitter vetch (vicia ervilia) and four diets including those legume seeds. in addition, the apparent digestibility of experimental diets was determined in goats. the legume seeds showed high protein content (206-319 g/kg dry matter). effective degrad ...201019138343
understanding the nutritional chemistry of lupin (lupinus spp.) seed to improve livestock production efficiency.in their raw, unprocessed form, lupins have many desirable characteristics for feeding both ruminants and single-stomached animals. an emphasis on these desirable characteristics when formulating diets, combined with an advanced knowledge of how components of lupins can influence nutritional value, will ensure they make a cost-effective contribution to livestock diets. the main lupin species used in livestock diets include lupinus albus, l. angustifolius and l. luteus. supplementation of ruminan ...199919087452
strains nodulating lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within bradyrhizobium.in this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating lupinus albus (white lupin) in several continents. collectively the analysis of their rrs and atpd genes, and 16s-23s intergenic spacers (its), showed that they belong to at least four chromosomal lineages within the genus bradyrhizobium. most isolates from the canary islands (near to the african continent) grouped with some strains isolated on mainland spain and were identified as bradyrhizobium ...201020135225
multilocus sequence analysis of the genus bradyrhizobium.the use of multilocus sequence analysis (mlsa) for the taxonomy of bradyrhizobium was assessed. we compared partial sequences for atpd, reca, gyrb, rpob and dnak for a set of reference strains representing named species and genospecies, and a number of new isolates from lupinus albus, arachis hypogaea and ornithopus compressus from spain. the phylogenies of the individual genes were compared with previous dna-dna hybridization results. high hybridization values were well reflected, but intermedi ...200919201125
cholesterol-lowering effects of dietary lupin (lupinus albus var multolupa) in chicken diets.the present study was undertaken to investigate the effect of different concentrations of lupin seeds (0, 200, and 400 g/kg), with and without cholesterol added (10 g/kg), in chicken diets on performance, relative liver weight, liver fat, intestinal ph and viscosity, and different blood serum parameters (glucose, cholesterol, triglycerides, total biliary salts, amylase, total protein and albumin, and globulin fractions). increasing the lupin content in the diet reduced weight gain and feed consu ...200718029810
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