| purification and properties of 5'-nucleotidase from lymphocyte plasma membranes. | 5'-nucleotidase is purified from lymphocyte plasma membranes by two affinity chromatographies. the first one, on lens culinaris lectin-sepharose 4b yields a fraction of twelve lectin-binding glycoproteins (lectin-receptor fraction). the second one on 5'-amp-sepharose 4b leads to pure enzyme. this enzyme is a glycoprotein with a molecular weight of 130 000; it gives a single band in polyacrylamide/dodecylsulfate electrophoresis and displays a very high specific activity (2500-3000 mumol pih-1mg-1 ... | 1978 | 28225 |
| structural characteristics of the alloantigens determined by the major histocompatibility complex of the guinea pig. | the gpla b and ia (i region-associated) antigens are the products of genes found in the guinea pig major histocompatibility complex. because of their importance in immune response phenomena, a structural study of these antigens was undertaken. [3h]leucine and [3h]fucose were internally incorporated into guinea pig lymph node cells. the gpla b and ia antigens were solubilized by the nonionic detergent nonidet p-40, purified by affinity chromatography using an adsorbent column of lentil lectin, is ... | 1976 | 55459 |
| purification of the thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. | the thy-1-molecule, which was identified by its antigenic activities, has been purified from rat thymocytes. the purification involved preparation of crude membranes and solubilization in deoxycholate, followed by gel filtration and affinity chromatography on antibody or lectin columns. in all cases the purified molecule was a glycoprotein that did not form higher polymers and was not associated with other polypeptide chains. the thy-1 glycoprotein could be found in two forms, one binding to len ... | 1975 | 56177 |
| purification of the thy-1 molecule from rat brain. | the present paper describes the characterization of the thy-1 molecule from rat brain. the molecule was recognized by its antigens, which could be solubilized from brain membrane with deoxycholate. in the solubilized form the thy-1 antigens were associated with a homogenous component with the following hydrodynamic properties: s20,w=2.2s,v=0.72ml/g and stokes radius=3.0nm. the mol.wt. of the deoxycholateantigen complex was estimated to be 27000; these values are not significantly different from ... | 1975 | 56178 |
| antigen-non-specific t-cell factor in b-cell activation. origin, biological properties and failure to show a relationship to h-2. | a population of t cells sensitive to als treatment release a non-specific factor (nsf) capable of replacing t cells in the response of nude spleen cells to erythrocyte antigens. this factor cannot be removed by immunoadsorbents with specificities directed towards products of the h2 complex, nor by lentil lectin, unlike certain specific t-cell factors. furthermore, it functions across histocompatibility differences between mouse strains. it has been directly demonstrated that this factor can infl ... | 1976 | 58834 |
| an improved method for isolation of h-2 and ia alloantigens with immunoprecipitation induced by protein a-bearing staphylococci. | mouse alloantigens, including the histocompatibility (h-2) and immune response linked (ia) antigen molecules, can be readily isolated by a new precipitation technique utilizing protein a-bearing, fixed staphylococci. the antigens are prepared by radiolabeling mouse spleen cells with 3h-leucine, solubilizing with the non-ionic detergent non-idet p-40, and purifying by affinity chromatography with lentil lectin coupled to sapharose. the antigen preparations are mixed with appropriate alloantisera, ... | 1976 | 58931 |
| the use of flurorescein investigations with concanavalin a, lens culinaris lectin and ricinus communis lectin. | investigations on methods for the utilization of fluorescein-isothiocyanate-labelled concanavalin a, lens culinaris lectin and ricinus communis lectin for immunohistological demonstration as simple sugar moieties are reported. the stainings were carried out on rabbit erythrocytes. ehrlich ascitee tumor cells and various mammalian tissues. optimum results were obtained in living cells and native cryostate tissue sections. the influence of different fixing agents and conditions of fixation on the ... | 1975 | 61131 |
| quantitative serological analysis of a rabbit anti-rat lymphocyte serum and preliminary biochemical characterisation of the major antigen recognised. | rabbit antisera to rat thoracic duct lymphocytes were examined by absorption analysis with quantitative radioimmunoassays to determine the tissue distribution of the antigens recognised and the amount of antibody against different specificities. seven of eight sera were remarkably specific for leucocytes and one serum was analysed in detail. in this serum about 70 per cent of the antibody was leucocyte specific and most was against determinants on thymocytes, bone marrow cells, peripheral lympho ... | 1977 | 68560 |
| detergent solubilization, purification, and separation of specificities of hla antigens from a cultured human lymphoblastoid line, rpmi 4265. | hla antigens have been purified to homogeneity after detergent solubilization from rpmi 4265, a human lymphoblastoid line. the inhibition of cytotoxicity assay for hla antigen was modified, using preincubation with bovine serum albumin of antigen samples containing detergent to prevent lysis of target cells by detergent. solubilization was tested with many types of detergents. a polyethyleneglycol oleyl ether nonionic detergent mixture, brij 99:brij 97 (2:1) was selected for solubilization, sinc ... | 1977 | 68955 |
| microheterogeneity of rat, mouse and human alpha1-fetoprotein as revealed by polyacrylamide gel electrophoresis and by crossed immuno-affino-electrophoresis with different lectins. | polyacrylamide gel electrophoresis and crossed immuno-affino-electrophoresis with several free lectins have been used to characterize and to compare the molecular heterogeneity of rat, mouse and human alpha1-fetoproteins. each alpha1-fetoprotein contains a variable number of electrophoretic variants depending on the gel porosity. in sds electrophoresis, two molecular size populations are present in rat alpha1-fetoprotein (mr = 74 000 and 72 000) and in mouse alpha1-fetoprotein (mr = 73 000 and 7 ... | 1979 | 83879 |
| the structure and evolution of the hla--bw4 and bw6 antigens. | the hla--bw4 and bw6 antigenic determinants have been shown to co-migrate with hla--b determinants on gel-filtration, sucrose density gradient centrifugation and affinity chromatography, using lens culinaris lectin and antibody against human beta 2 microbulin. these and other published data imply that the hla--bw4 and bw6 determinants reside on the same polypeptide chain as other hla--b locus determinants. the implications of this in terms of the evolution of cross reacting groups of antigens at ... | 1979 | 91209 |
| electron microscopic demonstration of a saccharide moieties in the hypophase of the alveolar surfactant system. | for electron microscopic demonstration of complex carbohydrates and simple sugars in the mouse lung, anionic dye and lectins were used. after immersion fixation of small lung tissue blocks, the alveolar surfactant system was destroyed and only membrane bound carbohydrates (cell coat) could be demonstrated by means of colloidal iron and ruthenium red. fixation of the whole lung via the visceral pleura preserved the alveolar surfactant system. only this technique afforded a distinction between cel ... | 1975 | 124909 |
| [lectin arthritis--a new arthritis model]. | by single intraarticular injection of lectin from lens culinaris in nosenitized rabbits acute and chronic arthritis was produced. by use of fluoresceinisothiocyanate-labelled lectin binding of lectin could be demonstrated in the connective tissue of joint capsule for 4 weeks. the morphological features of lectin-arthritis were described. the results are discussed with respect to the pathogenesis of the arthritis. | 1976 | 139098 |
| studies on t lymphocyte activation ii. the target cells for concanavalin a-induced growth factors. | growth factors contained in con a(concanavalin a)-conditioned media (cm) maintain exponential growth in t cell blasts derived from mitogen stimulation of spleen cells with con a, phytohemagglutinin, lentil lectin and pokeweed mitogen (pwm), as well as from mixed lymphocyte reactions to h-2d or i-c-encoded determinants and from non-h-2 mls locus-controlled reactions. such growth factors are strictly t cell blast-specific, inasmuch as they do not stimulate resting, small t or b lymphocytes nor b c ... | 1979 | 159185 |
| interaction of lens culinaris lectin, concanavalin a, ricinus communis agglutinin and wheat germ agglutinin with the cell surface of normal and transformed rat liver cells. | the observation of borek et al. (1973) on nonagglutinability of transformed rat liver cells by lens culinaris lectin and our ultrastructural findings of a greater mobility of the lens culinaris lectin receptors on transformed rat liver cells as compared to normal rat liver cells (roth 1975) initiated the present agglutination experiments on liver cells with lectins. for agglutination assay the microhemadsorption technique after furmanski et al. (1973) was used with exception of several tests on ... | 1975 | 183974 |
| enhancement of the pge1 response of macrophages by concanavalin a and colchicine. | the effect of concanavalin a (con a) and colchicine on the prostaglandin e1 (pge1)-mediated cyclic amp generation in rat peritoneal macrophages have been studied. although con a and colchicine by themselves did not affect cyclic amp levels, they greatly enhanced cyclic amp production induced by pge1. there was not only augmentation of cyclic amp levels at maximally active concentrations of pge1, but also an increased sensitivity to low (inactive) concentrations of pge1. except for lentil lectin, ... | 1977 | 196024 |
| purification of the fusion protein of sendai virus: analysis of the nh2-terminal sequence generated during precursor activation. | the two glycoproteins of sendai virus, the hemagglutinin-neuraminidase and the fusion protein (f), were separated and purified by affinity chromatography on a lens culinaris lectin-sepharose column. f was shown to consist of two disulfide-bonded glycopolypeptide chains, f1 and f2, of molecular weights 51,000 and 11,000, each of which contained 15% carbohydrate by weight. amino-terminal sequence analysis showed that f2 was blocked and that the hydrophobic sequence nh2-phe-phe-gly-ala-val-ile-gly- ... | 1978 | 208074 |
| developmental alteration of rat brain synaptic membranes. reaction of glycoproteins with plant lectins. | synaptic membranes were isolated from the forebrains of rats of increasing postnatal ages. developmentally related changes in the structure and concnetration of synaptic membrane glycoproteins were indicated by: (1) a 2--3 fold increase in glycoprotein sialic acid between 5 and 60 days; (2) a similar increase in the number of membrane receptors for the lectins concanavalin a and wheat germ agglutinin; (3) transient increases between 10 and 17 days in the receptors for lentil and castor bean lect ... | 1979 | 217493 |
| effects of concanavalin a on 5-hydroxytryptamine uptake by rabbit blood platelets and on their ultrastructure. | 1. effects of concanavalin a (con a) and other lectins on 5-hydroxytryptamine (5-ht) uptake by rabbit blood platelets and on their ultrastructure were studied. 2. uptake of [3h]-5-ht by platelets was decreased by application of con a, e-pha (lectin from phaseolus vulgaris) and lentil-pha (lectin from lens culinaris), but not by wheat germ agglutinin (wga). con a induced specific changes in the ultrastructure of platelets, causing (i) a change in external appearance from a discoid to an irregular ... | 1979 | 219926 |
| polypeptides of the epstein-barr virus membrane antigen complex. | epstein-barr virus (ebv)-associated membrane antigens have been purified from the plasma membranes of the producer cell line p3hr-1 nono. the antigens were assayed with a specific rabbit anti-ebv antiserum using an 125i-labeled staphylococcal protein a binding assay. the antigens have been shown to be present on purified plasma membranes. treatment of the plasma membranes with triton x-100 allows the separation of two antigenically distinct classes of antigens, one soluble and one insoluble in t ... | 1979 | 228070 |
| [common all-associated antigen on cells and in the serum of common all patients: clinical relevance and biochemical characterization (author's transl)]. | the clinical application of an antiserum recognizing common all associated antigen (call-ag) is very useful in classifying leukemias and diagnosing bone marrow relapse as well as cns-leukemia. we could demonstrate that sera of common all (call) patients contain call-ag; its partial biochemical characterization is described. the anti call serum (call-as) was raised in rabbits with call-cells precoated with rabbit antiserum against normal human lymphocytes. after appropriate absorbtion the call-as ... | 1979 | 286847 |
| cell-mediated mitogenic response induced by leukoagglutinin and lens culinaris lectin in mouse lymphocytes. | the proliferative response of mouse lymphocytes to syngeneic cellular stimulation upon membrane modification with lectins was studied. brief pretreatment of stimulator cells (mitomycin-c-treated spleen cells) followed by mixed culture with syngeneic cortisone-resistant thymocytes resulted in a significant proliferative response in the thymocytes. this effect was not due to a soluble mediator and was similar to the mitogenic response after con a-induced membrane modification reported previously. ... | 1977 | 302308 |
| isolation and characterizaiton of murine cell surface components. i. purification of milligram quantities of thy-1.1. | the thy-l.1 molecule was isolated from the bw5147 murine lymphoblastoid cell line. the initial step in purification was the preparation of a crude plasma membrane fraction followed by acetone precipitation. the acetone pellet was solubilized using deoxycholate (doc) and thy-1.1 was purified by use of a lens culinaris lectin affinity column and an aca-34 gel filtration column. the purified glycoprotein with thy-1.1 activity had a mol wt of approximately 25,000 daltons. the isolation of this molec ... | 1977 | 302318 |
| glycoproteins and antigens of membranes prepared from rat thymocytes after lysis by shearing or with the detergent tween-40. | in purification of cell surface antigens an efficient method for preparing membrane from large numbers of cells is needed. such a method is described for preparing membranes from rat thymocytes after lysis in the non-ionic detergent tween-40. cell surface antigens were recovered at a yield of 30-50%, and a purification of 30-40-fold. by contrast enzyme markers for the other cell organelles were present in the membrane fraction in very low yield. the membrane obtained with the detergent method wa ... | 1978 | 305263 |
| actin may be present on the lymphocyte surface. | lymphocytes were assessed for the presence of surface actin and myosin by lactoperoxidase-catalyzed iodination and indirect immunofluorescence using antisera against purified pig skeletal muscle actin and pig smooth muscle myosin. sodium dodecyl sulfate/polyacrylamide gel electrophoresis of 125i-labeled pig, mouse, and human b lymphocytes revealed an intense radioactive band of 43,000 molecular weight, whereas pig and mouse t lymphocytes gave a much less intense band. this band comigrated with a ... | 1978 | 309133 |
| interaction of cytotoxic t lymphocytes with target cells. i. specific inhibition by detergent-solubilized, partially purified mouse histocompatibility antigens. | h-2 antigens from el4 (h-2b) and rlmale 1 (h-2d) leukemias were solubilized with deoxycholate and partially purified based on their adherence to a lens culinaris hemagglutinin column. in double reciprocal experiments we have shown that these preparations specifically inhibit conjugate formation between target cells and alloimmune peritoneal lymphocytes, a preparation rich in cytotoxic t lymphocytes. | 1978 | 309895 |
| human platelet membrane receptor for bovine von willebrand factor (platelet aggregating factor): an integral membrane glycoprotein. | the platelet membrane receptor for bovine von willebrand factor, platelet aggregating factor, has been reported to be a property of a soluble glycoprotein, glycocalicin, that is loosely attached to the platelet surface and represents one of the major glycoproteins of the platelet glycocalyx. the studies reported here, however, demonstrate that fractions from human platelets containing glycocalicin have no bovine von willebrand factor receptor activity. instead, only fractions containing platelet ... | 1979 | 312498 |
| au cell-surface antigen of human malignant melanoma: solubilization and partial characterization. | au antigen is defined by reactions of sera from patient au with cell-surface antigens of cultured autologous melanoma cells (sk-mel-28). past studies established that no available cell type other than au melanoma expressed au antigen. by use of antibody inhibition tests for antigen detection, limited papain digestion of au melanoma cells was found to result in the solubilization of au antigen along with beta2-microglobulin (beta 2m) and hla allogeneic and xenogeneic specificities. comparable pap ... | 1979 | 313568 |
| further characterization of a human t lymphocyte associated antigen. | a human t lymphocyte associated antigen (tlaa), defined with appropriately absorbed antisera to the lymphoblastoid cell line hsb, was originally characterized as a 150,000 mol. wt antigen using sodium deoxycholate solubilized, 125i bolton-hunter labelled membranes of hsb, thymus and peripheral blood lymphocytes. the tlaa, which appears to be the major membrane protein labelled with either the bolton-hunter, or galactose oxidase-sodium 3h borohydride methods, is weakly labelled in vitro with a 3h ... | 1979 | 315289 |
| surface glycoproteins from human t, b and chronic lymphatic leukemia lymphocytes isolated by 3 different lectins. | glycoproteins from the surface of human t. b and leukemic lymphocytes were isolated and partially characterized by using three different lectins, lens culinaris lectin (lc), wheat germ agglutinin (wga) and phytohemagglutinin from phaseolus vulgaris (pha). cells were labelled either with i125 or 3h-fucose and lysed with detergent. labelled components were isolated by incubation with lectins and anti-lectin antisera and immune precipitates were analysed by sds-polyacrylamide gel electrophoresis. t ... | 1979 | 316713 |
| the lectin binding sites on the plasma membrane components of human lymphoblastoid cell lines. | the plasma membrane components of five human b-cell lines and three human t-cell lines were separated by dodecyl sulfate polyacrylamide gel electrophoresis, incubated with the radioactive labeled lectins from lentil, castor bean, wheat germ, phaseolus bean, peanut, gorse and the roman snail and the molecular weights of the binding sites determined. the lentil, castor bean and wheat germ lectin bound to multiple components from molecular weights (mr) 20 000 to 200 000 within the plasma membranes, ... | 1979 | 316801 |
| molecular structure of human histocompatibility antigens: the hla-c series. | the hla-cw2 antigen of the b lymphoblastoid cell line bri 8 is structurally homologous to the hla-a and b antigens as judged by various criteria. each antigen comprised a glycosylated polypeptide of 43 000 molecular weight that is noncovalently associated with beta2-microglobulin (beta2m). some small differences in molecular parameters were, however, revealed. thus, the deoxycholate-solubilized hla-cw2 antigen sedimented at the same rate as the hla-a antigens but at a slightly faster rate than t ... | 1977 | 332508 |
| the use of fluorescein isothiocyanate labelled lectins for immuno-histological demonstration of saccharides. iii. studies by use of ricinus communis lectin and wheat germ agglutinin. | cryostat sections of heart, skeletal muscle, spleen, liver, cartilage, brain, nerve, tongue, esophagus and salivary gland from man, rabbit, rat and guinea pig were stained with fluorescein isothiocyanate labelled ricinus communis lectin and wheat germ agglutinin. the stained sections were examined by fluorescence microscopy. the general pattern of fluorescence is very similar to that obtained with labelled lens culinaris lectin and concanavalin a. particularly the lectins stain connective tissue ... | 1977 | 338333 |
| human cell membrane components bound to beta2-microglobulin in t cell-type cell lines. | cell membrane components bound to beta2-microglobulin were isolated from renex 30 (a nonionic detergent)-solubilized membrane materials of two human t cell-type cell lines, molt-4 and ccrf-cem, by gel filtration and lectin affinity chromatography. the isolation was carried out by following the beta2-microglobulin activity by radioimmune inhibition assay. the t cell membrane components bound to beta2-microblogulin had a uniform molecular size of about 200,000 daltons and most of them showed an af ... | 1978 | 340586 |
| immunoprecipitation and partial characterization of diphtheria toxin-binding glycoproteins from surface of guinea pig cells. | 125i-labeled membrane glycoproteins that specifically interact with diphtheria toxin and crm197 protein--but not with diphtheria toxoid, fragment a of diphtheria toxin, or cholera toxin--were detected by use of the lactoperoxidase labeling technique followed by an immunoprecipitation system. these glycoproteins, which adhere to lentil lectin-sepharose columns, are present on the surface of diphtheria toxin-sensitive guinea pig lymph node cells but are completely lacking on the surface of diphthe ... | 1979 | 370834 |
| purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. | a leukocyte-common (l-c) antigen which can be dominant as an immunogen in rabbit anti-rat thoracic duct lymphocyte serum has been purified from rat thymocytes. initially, an antigenic fragment of 100,000 apparent mol. wt. was prepared at 400 to 900-fold purification by lentil lectin affinity chromatography and gel filtration in deoxycholate. mice were then immunized with this fraction, and a hybrid myeloma cell line secreting antibody to the l-c antigen was prepared by cell fusion. this antibody ... | 1979 | 374095 |
| fractionation of human lymphocytes with plant lectins. ii. lens culinaris lectin and wheat germ agglutinin identify distinct lymphocyte subclasses. | | 1979 | 379219 |
| lentil-lectin affinity chromatography of surface glycoproteins and the receptor for ige from rat basophilic leukemia cells. | surface proteins and glycoproteins of rbl cells were labelled enzymatically with 125i and then solubilized with nonidet p-40. analysis by polyacrylamide-gel electrophoresis in sds on 10% gel revealed 10 distinctive peaks ranging in molecular weights from 17,000 to 200,000 daltons. mainly components of higher molecular weights were bound by lentil-lectin sepharose and could be eluted with alpha-methyl mannoside. the receptor for ige was clearly shown to bind to the lentil-lectin. a second cell su ... | 1979 | 422283 |
| distribution of cell surface saccharides on pancreatic cells. ii. lectin-labeling patterns on mature guinea pig and rat pancreatic cells. | the surface saccharide composition of collagenase-dispersed pancreatic cells from adult guinea pig and rat glands was examined by using eight lectins and their ferritin conjugates: concanavalin a (cona); lens culinaris (lcl); lotus tetragonolobus (ltl); ricinus communis agglutinins i and ii (rca i, rca ii); soybean agglutinin (sba); ulex europeus lectin (uel); and wheat germ agglutinin (wga). binding studies of iodinated lectins and lectin-ferritin conjugates both revealed one population of satu ... | 1979 | 422654 |
| fractionation of human lymphocytes with plant lectins. i. structural and functional characteristics of lymphocyte subclasses isolated by an affinity technique using lens culinaris lectin. | | 1979 | 466715 |
| associative symbiosis of azotobacter chroococcum and higher plants. | the association between a selected strain of azotobacter chroococcum and seven plants was investigated in water cultures under sterile conditions. azotobacter population progressively increased in the nutrient solution and on the rhizoplane. microbial propagation depends on the type of plant, being much higher in presence of wheat, followed by barley, maize, broad bean, and cotton, while in presence of fenugreek and lentil lower rates of multiplication were recorded. inoculation increased the dr ... | 1979 | 473964 |
| interaction of lectins with human platelets. effects on platelet stimulation by thrombin and ristocetin. | wheat germ agglutinin induced aggregation and secretion of fresh platelets. aggregation, but not secretion of serotonin by platelets in plasma, by the lectin was inhibited by 5 mm edta. further, the lectin-induced stimulation of fresh platelets was blocked by prostaglandin e1. thus, this lectin stimulates platelets by a mechanism which closely mimics thrombin activation and is independent of intercellular crosslinking. lentil lectin did not stimulate platelets. each platelet contained about 6 . ... | 1979 | 476155 |
| the purification of murine histocompatibility antigens (h-2b) from rbl-5 tumor cells using detergents. | a detergent-solubilized form of h-2b (dh-2b) has been purified 1500-fold from rbl-5 tumor cells. the purification was accomplished by deoxycholate solubilization of purified plasma membranes, gel filtration, lens culinaris lectin affinity chromatography, and affinity chromatography on a sheep anti-dh-2b immunoadsorbent. both alloantigen and beta 2-microglobulin were monitored by radioimmunoassay during purification. the final product was judged to be greater than 90% pure by the following criter ... | 1979 | 500630 |
| age-related cell surface changes in human diploid fibroblasts revealed by lectin-mediated red blood cell adsorption assay: a lectin survey. | two types of age-related cell surface changes could be demonstrated in human diploid fibroblasts with the two methods of the lectin-mediated red blood cell (rbc) adsorption assay: the fibroblast coating method (in which rbcs are adsorbed to lectin-coated fibroblasts) and the rbc coating method (in which lectin-coated rbcs are adsorbed to fibroblasts). with the fibroblast coating method, concanavalin a and agglutinin l from phaseolus vulgaris gave a change in rbc adsorption which did not occur th ... | 1979 | 522510 |
| the lectin binding sites on the membranes of the nuclear envelope, mitochondria and the cell surface of human lymphoblastoid cells. | the membranes of the cell surface, the endoplasmic reticulum, outer and inner mitochondrial leaflet and nuclear envelope were isolated from three human lymphoblastoid cell lines. membrane components were separated by dodecyl sulfate polyacrylamide gel electrophoresis and the gels incubated with the radioiodinated lectins from lentil, castor bean, scarlet runner bean, gorse seed and roman snail. after gel slicing and counting, the molecular weights of the lectin binding sites were determined. abo ... | 1979 | 527941 |
| the binding of lectins to components of plasma membranes from porcine submaxillary lymph node lymphocytes. | by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis the plasma membranes from porcine lymphocytes contain at least 30--35 glycopolypeptides and one or more glycolipids to which one or more of 12 purified lectins bind. the specificities of binding generally followed the same pattern as those of the reaction of the lectin with intact pig lymphocytes. some lectins (e.g., the isolectin pair, agaricus bisporus lectins a and b and a group consisting of the lens culinaris a and b isol ... | 1978 | 568485 |
| transplantation in minature swine. iv. chemical characterization of msla and ia-like antigens. | immunochemical analyses of radioactively labeled lymphocyte antigens from miniature swine of three different homozygous major histocompatibility (mhc) types, aa, cc, and dd, have been performed. anti-mhc sera were incubated with lentil lectin purified nonidet p-40 swine lymphocyte extracts. antigen-antibody complexes were then precipitated with protein a bearing staphylococci, eluted, and electrophoresed on sodium dodecyl sulfate polyacrylamide gels. analysis of antigens from aa or dd cells reve ... | 1978 | 621396 |
| labeling characteristics and separation of ia antigen subunits. | experiments with biosynthetic incorporation of 3h-amino acids into murine and guinea pig ia antigens have indicated that these antigens consist of two polypeptide chains of 33,000 and 25,000 daltons, respectively, occasionally linked by disulfide bonds into a 58,000 dalton molecule. in contrast, studies with lactoperoxidase-catalyzed radioiodination have indicated that these ia antigens consist of only a single chain of 25,000 daltons. we therefore undertook a study to explore the basis of these ... | 1978 | 621402 |
| [temporal characteristics of the movement of the cut-off elements of artificial heart valves in a pulsing flow]. | a comparative investigation of time characteristics of the movement of artificial heart valves with spherical, hemispherical and lentil-shaped obturative elements under non-stationary conditions was carried out. it has been found that it is practically impossible to avoid the delay in the valve closing and opening, as well as the regurgitation phenomena, on the valve prothesis, due to the inertia of obturative elements and attached masses. a searching for new models of the heart valves should go ... | 1978 | 653995 |
| spatial orientation of glycoproteins in membranes of rat liver rough microsomes. i. localization of lectin-binding sites in microsomal membranes. | carbohydrate-containing structures in rat liver rough microsomes (rm) were localized and characterized using iodinated lectins of defined specificity. binding of [125i]con a increased six- to sevenfold in the presence of low doc (0.04--0.05%) which opens the vesicles and allows the penetration of the lectins. on the other hand, binding of [125i]wga and [125i]rca increased only slightly when the microsomal vesicles were opened by doc. sites available in the intact microsomal fraction had an affin ... | 1978 | 701363 |
| equilibrium and kinetic studies of the binding of lens culinaris lectin to rabbit erythrocytes by a quantitative fluorometric method. | the binding of lens culinaris lectin to the receptors of rabbit erythrocytes was studied by a quantitative fluorometric method using the fluoresceinated conjugate. equilibrium data showed the presence of 1.2 x 10(6) receptors/cell with an association constant varying from 8 to 3 x 10(6) m-1 over a temperature range of 5 to 37 degrees c. the binding reaction is exothermic with a deltah = -4.6 kcal/mol and a deltas = + 15 eu. it appears that most of the bound lectin molecules had only a single sit ... | 1978 | 718870 |
| human placental 5'-nucleotidase and alkaline phosphatase. receptors for concanavalin a and lens culinaris lectin. | | 1978 | 730116 |
| [nutritional evaluation of protein concentrates of beans (phaseolus vulgaris) and lentils (lens esculenta)]. | the composition and nutritive value were determined in navy bean meal (phaseolus vulgaris) and lentil meal (lens esculenta), and in their respective protein concentrates obtained through extraction followed by isoelectric precipitation. sulfur amino acids per gram of nitrogen were lower in the concentrates than in the meals, while there was no difference for lysine and threonine. the white bean protein concentrate had a lower biological value than the meal but better digestibility, although tryp ... | 1978 | 753176 |
| the use of fluorescein isothiocyanate labelled lectins for immunohistochemical demonstration of saccharides. | cryostat sections of various tissues from man, rat, mice, hamster, rabbit, guinea pig, sparrow and carp were examined by fluorescence microscopy after incubation with fluorescein isothiocyanate labelled concanavalin a and lens culinaris lectin. in the same tissue of various species no basic differences in the pattern of fluorescence were observed. in general, connective tissue structures were stained by both lectins in the same degree, whereas by fluorescein isothiocyanate labelled concanavalin ... | 1975 | 786713 |
| histochemical lectin affinity technique by means of fitc-labeled serum protein fractions. | a two-step affinity technique is described for light microscopic demonstration of the concanavalin a, agaricus bisporus lectin and ricinus communis lectin binding sites by means of various fitc-labeled human and rabbit serum protein fractions. experiments for the visualization of the lens culinaris lectin and the pisum sativum lectin binding sites gaves negative results. the technique consist of two reaction steps which involve the incubation of tissue sections in the lectins followed by the vis ... | 1976 | 791903 |
| immunogenic properties of eukaryotic ribosomal proteins. | pure rat, chicken and kidney bean ribosomal proteins are immunogenic in the rabbit as tested by the ouchterlony double diffusion technique. on the other hand, we were unable to raise antibodies to rabbit ribosomal proteins in the guinea pig. rabbit ribosomal proteins are not autoimmunogenic. rabbit antisera to rat or chicken ribosomal proteins surprisingly give no cross-reaction with ribosomal proteins from other mammals (man, ewe, calf, guinea pig, mice) whereas antisera to kidney bean ribosoma ... | 1975 | 804929 |
| spatial arrangement of some erythrocyte membrane receptor sites. | alcian blue proved to be a hemagglutinin specific for negatively charged receptors. either sialylization or tryptic degradation of glycoproteins of human erythrocytes greatly diminished the agglutinability by alcian blue. studies with group a antiserum, anti-ahp and the lectin of lens culinaris demonstrated the masking efficiency of erythrocyte major glycoproteins. previous proteolytic digestion enhanced the agglutinability of human red blood cells due to uncovering of what is considered glycoli ... | 1976 | 823773 |
| [fluorescence microscopic demonstration of human and animal connective tissue structure by means of fitc-labeled lentil agglutinin]. | | 1975 | 829940 |
| synaptic plasma membrane glycoproteins: molecular identification of lectin receptors. | synaptic plasma membranes isolated from rat cerebral cortex reacted with concanavalin (con a), wheat germ agglutinin (wga), lens culinaris phytohaemagglutinin (lch), and ricinus commounus agglutinin (rca). competition studies indicated that specific topographical relationships exist between receptors for con a and lch and for wga and lch. reaction of [125i]con a with synaptic membrane proteins following polyacrylamide gel electrophoresis identified eight distinct molecular weight classes of glyc ... | 1977 | 836792 |
| isolation of the membrane glycoproteins of human blood platelets by lectin affinity chromatography. | the major platelet membrane glycoproteins have been solubilized in 1.0% sodium deoxycholate and subjected to affinity chromatography on the lectins from lens culinaris, wheat germ and abrus precatorius. polyacrylamide gel electrophoresis in the presence and absence of a reducing agent together with the differential binding of the lectins to the glycoproteins permitted the distinction of at least seven separate glycoprotein entities. a new nomenclature for the glycoproteins is proposed to accomod ... | 1977 | 836823 |
| purification of the glycoprotein lectin from the broad bean (vicia faba) and a comparison of its properties with lectins of similar specificity. | 1. the lectin from the broad bean (vicia faba) was purified by affinity chromatography by using 3-o-methylglucosamine covalently attached through the amino group to ch-sepharose (an omega-hexanoic acid derivative of agarose). its composition and the nature of its subunits were compared with concanavalin a and the lectins from pea and lentil. 2. unlike the other three lectins, broad-bean lectin is a glycoprotein; a glycopeptide containing glucosamine and mannose was isolated from a proteolytic di ... | 1976 | 938471 |
| nutritive studies on some raw and prepared leguminous seeds commonly used in the arab republic of syria. | 1. the proximate analysis of raw syrian lentils (lens esculentus), variety red chick pea (cicer arietinum) variety balady, has been made. the protein content of the two raw seeds were 23 and 22 g% for lentils and chick peas, respectively. ethereal extract, fiber, ash, calcium, phosphorus and iron content of the two raw seeds have been also assayed. 2. the levels of most of the amino acids were also estimated in the raw and cooked seeds. it was found that tryptophan- and sulphur-containing amino ... | 1976 | 1020373 |
| effect of phosphate fertilization and seed inoculation with ""okadin'' at high rate on yield of broadbean and lentil. | the effect of phosphate fertilization and seed inoculation with ""okadin'' preperation of rhizobia strains on the yield of broadbean and lentil was tested in field experiments during the seasons 1969 to 1972 in the farm of faculty of agriculture, assiut university. superphosphate was added at the rate of 0, 50, 100, or 200 kg/fa., and ""okadin'' preparation, specific for the kind of legume planted, was applied to the seeds at 10 times the recommended rate. application of superphosphate at any of ... | 1976 | 1037057 |
| interaction of plant lectins with purified human lymphocyte populations: binding characteristics and kinetics of proliferation. | with six purified plant lectins as probes of cell surface structure, human t, b, and null lymphocytes have been examined for cell surface differences. the results of binding experiments with radioiodinated lectins indicate that these three lymphocyte populations, although functionally different, bind equivalent amounts of e- and l-pha, lentil pha, con a, rca-i, and wga. since these lectins are capable of binding to at least four different cell surface oligosaccharides, these data indicate that h ... | 1975 | 1079038 |
| the chitin-glucan complex of saccharomyces cerevisiae. iii. electron-microscopic study of the prebudding stage. | differentiation of the cell wall of saccharomyces cerevisiae at the site of the future bud was followed. a lentil-like structure originates on the inner side of the cell wall during the first phase. at the same time, an electron-dense layer occurs at the boundary between the inner layer of the cell wall and the lentil-like structure. during the second phase granular material is accumulated at the lower side of the lentil-like structure. during the third phase the lentil-like structure is split a ... | 1975 | 1104422 |
| lectins from wistaria floribunda seeds and their effect on membrane fluidity of human peripheral lymphocytes. | two lectins were isolated from wistaria floribunda seeds. one is a strong mitogen against human peripheal lymphocytes and has been purified in the previous paper (toyo-shima s., y., nakano k., tonomura, a., and osawa t. (1971) biochemistry 10, 4457). the other, which is a strong hemagglutinin being devoid of mitogenic activity against normal lymphocytes, has been purified in this paper by affinity chromatography on a sepharose 6b column followed by deae-sephadex column chromatography. both lecti ... | 1975 | 1112822 |
| studies on phytohemagglutinins. xxvi. a new type of a lentil hemagglutinin isolated from lens esculenta moench., subsp. microsperma (baumg.) barulina. | in seeds of the lentil lens esculenta moench., subsp. microsperma (baumg.) barulina, a nonspecific phytohemagglutinin was found, the properties of which differ from those of the previously described lentil hemagglutinins. the phytohemagglutinin is not adsorbed to the sephadex matrix; its hemagglutinating activity is different towards erythrocytes of different human blood groups of the abo system. the isolated phytohemagglutinin preparation is homogeneous by disc polyacrylamide and starch gel ele ... | 1975 | 1138920 |
| ultrastructural detection of lectin receptors by cytochemical affinity reaction using mannan-iron complex. | a two-step affinity reaction is described for electron microscopic demonstration of the concanavalin a as well as the lens culinaris lectin receptors by means of the yeast mannan-iron complex. first the tissue was incubated in the lectin. afterwards the incubation in the yeast mannan-iron complex was performed and reaction takes place between the still free second sugar binding site of membrane bound lectin molecules and the polysaccharides. this membrane receptor-lectin-polysaccharide complex i ... | 1975 | 1141022 |
| electron microscopic demonstration of cell surface carbohydrates by means of peroxidase and ferritin complexes of the lens culinaris lection. | the use of lens culinaris lectin for electron microscopic detection of d-mannose,- d-glucose and n-acetyl-d-glucosamine like sites on tumor cells, erythrocytes, erythrocyte ghosts, cultured rat liver cells and various tissues of mice is demonstrated. in addition to lens culinaris lectin-peroxidase reaction (lel-po reaction) the preparation of active lens culinaris lectin-ferritin conjugate are described and the specificity of cytochemical reactions are demonstrated. furthermore experiments by im ... | 1975 | 1150486 |
| the effect of volatile and gaseous metabolites of swelling seeds on germination of fungal spores. | effects of volatile and gaseous metabolites of swelling seeds of pea, bean, wheat, corn cucumber, tomato, lentil, carrot, red papper and lettuce on germination of spores of five genera of fungi were found to depend rather on the fungal than on the plant genus. germination of spores of botrytis cinerea, mucor racemosus and trichoderma viride was most severely inhibited. spores of verticillium dahliae were less sensitive and germination of spores of fusarium oxysporum was inhibited only in two cas ... | 1975 | 1176038 |
| lens culinaris lectin receptors in the plasma membrane of rat liver cells: comparative electron microscopic studies on normal cells, on cells in vivo transformed by diethylnitrosamine and on zajdela ascites hepatoma cells. | the distribution of the lens culinaris lectin receptors on normal rat liver cells, on rat liver cells in vivo transformed by diethylnitrosamine and on zajdela ascites hepatoma cells of the rat is investigated by means of the lens culinaris lectin-peroxidase method and by ferritin conjugated lens culinaris lectin. the normal rat liver cells show a continuous labeling at the outer membrane surface by the lectin complexes, whereas the transformed rat liver cells exhibit a strong tendency for patchy ... | 1975 | 1233303 |
| partial purification of the cystic fibrosis transmembrane conductance regulator. | we have investigated several purification strategies for the cystic fibrosis transmembrane regulator (cftr) based on its structural similarity to other proteins of the traffic atpase/abc transporter family. recombinant cftr expressed in heterologous cells was readily solubilized by digitonin and initially separated from the majority of other cellular proteins by sucrose density gradient centrifugation. cftr, with two predicted nucleotide binding domains, bound avidly to several triazine dye colu ... | 1992 | 1281484 |
| changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding. | lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) c57 bl/6j mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. the following lectins were used: canavalia ensiformis con a, triticum vulgaris (wga), glycine max (sba), griffonia simplicifolia (gs ii), arachis hypogaea (pna), pisum sativum (psa) and lens culinaris (lca). after incubation of frozen sections with con a, wga, gs ii, psa and lca a sarcoplasmic staining was obse ... | 1992 | 1290587 |
| antibodies to recombinant-derived glycoprotein b after natural human cytomegalovirus infection correlate with neutralizing activity. | glycoprotein b (gb) of human cytomegalovirus (hcmv) was partially purified by lentil-lectin column chromatography from cells infected with an adenovirus-gb recombinant. this antigen, which contained specifically reactive proteins of approximately 130 and 55 kda, was used to investigate gb antibody levels after natural hcmv infection in 48 individuals. all sera had igg antibody to gb as detected by radioimmunoprecipitation (rip) assays. quantitative rip showed a strong correlation between gb anti ... | 1992 | 1309850 |
| purification and characterization of human lymphoblast n-acetylglucosamine-1-phosphotransferase. | n-acetylglucosamine-1-phosphotransferase (glcnacptase) was solubilized with 2% tergitol np-10 from cultured human lymphoblast cells and purified 3840-fold with 14% recovery using lentil lectin-sepharose 4b, deae-sephacel and sephacryl s-400 chromatographies. the partially purified enzyme requires the non-ionic detergent tergitol np-10 and a divalent cation, mn2+ or mg2+, for its activity and exhibits an optimal ph at 7.2-7.5 in tris-maleate buffer. kinetic studies demonstrated an apparent km of ... | 1992 | 1318762 |
| incomplete digestion of legume starches in rats: a study of precooked flours containing retrograded and physically inaccessible starch fractions. | the digestibility of starch in precooked flours from green coat lentils (lens culinaris medik) and red kidney beans (phaseolus vulgaris l.) was investigated by balance experiments using rats treated with antibiotics to suppress hind-gut fermentation. the legume preparations were rich in intact cells filled with denaturated starch and contained retrograded amylose. between 8% (beans) and 11% (lentils) of the total starch ingested appeared in the feces, indicating a relatively low starch digestibi ... | 1992 | 1320113 |
| characterization of mineral-binding 40-kda glycoprotein extracted from young adult rabbit alveolar bone. | a forty-kilodalton (40-kda) protein was extracted from alveolar bone of young adult rabbit with 0.5 m edta after extraction with 4 m guhcl, and purified by gel-filtration, anion-exchange and hydroxyapatite columns using a high-pressure liquid chromatography system under denaturing conditions. the purified 40-kda protein was not susceptible to bacterial collagenase and thrombin, but was cleaved by cyanogen bromide. the protein was stained blue with stains-all. among various lectins, concanavalin ... | 1992 | 1323644 |
| oligosaccharide composition of the neurotoxin-responsive sodium channel of mouse neuroblastoma and requirement of sialic acid for biological activity. | a glycoprotein, m(r) 200,000, which has the biological activity of the neurotoxin-responsive na+ channel, was isolated from a clonal line of mouse neuroblastoma cells, n-18. the glycoprotein was purified to homogeneity in 18% yield by methods used to purify glycoproteins, which included metabolic labeling of the cells with l-[3h]fucose and binding of the radioactive glycoproteins to wga- and lentil-sepharose, and deae-cellulose. the glycoprotein has biological activity of neurotoxin-responsive i ... | 1992 | 1337866 |
| influence of different substrates in detoxification activity of adult rat hepatocytes in long-term culture: implications for transplantation. | substrates used to immobilize hepatocytes for transplantation govern attachment and long-term metabolic activity of these cells. the choice of these substrates is based on the familiarity with proteinaceous materials that are constituents of the extracellular matrix. the use of substrates that recognize carbohydrates on the cell surface may provide an alternative method to attach adult mammalian hepatocytes. in this study, immobilized lectins on tissue culture plasticware were used to support he ... | 1992 | 1344292 |
| impact of dietary protein and fat source on the development of insulin-dependent diabetes in the bb rat. | epidemiological studies show a remarkable geographical difference in the prevalence of iddm, suggesting a role for environmental factors such as diet, infection, or stress in the etiology of the disease. dietary modification has already been shown to be effective in the prevention of autoimmune diabetes in the bb rat and nod mouse. we studied the effect of protein and fat source in the prophylaxis of diabetes in the bb rat. natural ingredient rat chow was consistently associated with a high expr ... | 1992 | 1345000 |
| duck lymphocytes--v. transformation responses to phorbol ester and calcium ionophore. | 1. lymphocytes purified from duck blood and spleen were cultured in the presence of phorbol 12-myristate 13-acetate (pma) and the calcium ionophore a23187. stimulation was assessed by the incorporation of [3h]thymidine after 3 days' culture. 2. pma stimulated over a wide range of concentrations, with maximum stimulation at final concentrations of 5 x 10(-7)-5 x 10(-8) m/litre. a23187 was effective in the range 5 x 10(-6)-5 x 10(-7) m/litre and also, in some experiments using spleen lymphocytes, ... | 1992 | 1354147 |
| cd4-binding of gp130 micelles isolated from sivagmtyo-7. | the binding and binding inhibition of the sivagmtyo-7 external glycoprotein gp130 in micellar form to the cd4 molecule on human molt-4 clone 8 cells was investigated. the best binding of gp130 to molt-4 clone 8 cells occurred at ph 5.5 to 6.5 at 37 degrees c after 4 h or at room temperature after 10 h. the dissociation constant of this reaction was 0.2-0.4 nm, with both soluble cd4 or cd4 on molt-4 clone 8 cells. this value is close to 0.15 nm determined for the antihuman cd4 monoclonal antibody ... | 1992 | 1354467 |
| storage of glycoprotein in nctr-balb/c mouse. lectin histochemistry, and biochemical studies. | a strain of balb/c mice carrying a lysosomal storage disorder exhibits metabolic and phenotypic abnormalities similar to patients with sphingomyelin-cholesterol lipidoses type ii (i.e., niemann-pick c and d). their foamy cells, which belong to the reticuloendothelial system, stained intensely by periodate-schiff (pas) reagent and were resistant to predigestion with diastase. to identify the chemical nature of the pas-positive storage material, we applied lectin histochemistry and biochemical met ... | 1992 | 1360721 |
| purification of rat liver arylsulfatase a and its microheterogeneity assayed by crossed affinity-immunoelectrophoresis. | arylsulfatase a (arylsulfatase sulfohydrolase) ec 3.1.6.1 was purified from rat liver by a procedure consisting of differential centrifugation, con a-sepharose and blue sepharose chromatography, pbe 94 chromatofocusing, deae-cellulose and gel filtration chromatography followed by preparative electrophoresis. a molecular mass of 132,000 was estimated by gradient page. particular proteins were detected by immunoelectrophoresis. isoelectric focusing combined with immunoelectrophoresis gave two peak ... | 1992 | 1363453 |
| altered glycosylation of alpha-fetoprotein in hepadnavirus-induced hepatocellular carcinoma of the woodchuck. | altered glycosylation of alpha-fetoprotein (afp) has been proposed as a marker of hepatocellular carcinoma (hcc) in humans. the lectin-binding properties of woodchuck afp were investigated to determine if woodchuck hepatitis virus (whv)-induced hccs are also accompanied by changes in afp glycosylation. ninety-eight to 100% of the afp from normal, whv-free woodchucks with physiologic afp elevations and from whv-carrier woodchucks with hcc bound to concanavalin a, indicating that virtually all of ... | 1992 | 1373341 |
| establishment of a human hepatoblastoma model in athymic nude mice. producibility of fatty acid-binding protein, alpha-fetoprotein, and alpha 1-acid glycoprotein. | an athymic nude mouse model of human hepatoblastoma, designated hbl-2, was established. hbl-2 produced alpha-fetoprotein (afp) and the serum level in tumor-bearing mouse was roughly proportional to the tumor weight. reactivity of afp to concanavalin a and lens culinaris lectins indicated that it contained predominantly fucosylated, non-bisecting n-acetylglucosamine as a sugar moiety. alpha 1-acid glycoprotein (aag) was also found in both hbl-2 tumor extract and sera of tumor-bearing mice. the se ... | 1992 | 1376954 |
| glycosylation changes in human chorionic gonadotropin and free alpha subunit as gestation progresses. | carbohydrate is important to the structure, function, and circulatory survival of the glycoprotein hormones. human cg (hcg) and the related free alpha-molecule of pregnancy contain four and two asparagine-linked oligosaccharides, respectively. the present study analyzes changes in the glycosylation patterns of hcg and free alpha in early vs. late gestation. five volunteers provided 24-h urine samples, weekly, throughout their pregnancies. extracts of early pregnancy (weeks 7-12) and late pregnan ... | 1992 | 1377707 |
| interaction of lectins with human ige: ige-binding property and histamine-releasing activity of twelve plant lectins. | we examined the ige-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin a (con a), lens culinaris hemagglutinin (lch), pisum sativum agglutinin (psa), wheat germ agglutinin (wga), soybean agglutinin (sba), bauhinia purpurea agglutinin (bpa), peanut agglutinin (pna), ricinus communis agglutinin i (rca-i), lotus tetragonolobus agglutinin (lotus a), ulex europeus agglutinin i (uea-i), phytohemagglutinin e (pha-e) and phytohemagglutinin l (pha-l) ... | 1992 | 1378039 |
| growth of a human yolk sac tumor cell line with yolk sac-derived functions in selenium-supplemented chemically defined synthetic medium. | a human yolk sac tumor cell line, tg1, which was established from a testicular yolk sac tumor, was found to replicate continuously in a chemically defined medium supplemented with na2seo3 (isrpmi). tg1 produced several plasma proteins and growth factors: albumin, alpha-fetoprotein (afp), ferritin, carcinoembryonic antigen, beta-2-microglobulin, polyamine, neuron specific enolase, tissue polypeptide antigen, transferrin (tf), epidermal growth factor, and platelet derived growth factor. by analysi ... | 1992 | 1378830 |
| synthesis of 1-n-glycyl beta-oligosaccharide derivatives. reactivity of lens culinaris lectin with a fluorescent labeled streptavidin pseudoglycoprotein and immobilized neoglycolipid. | the lectin from lens culinaris (lentil) has a binding specificity for glycopeptides bearing 6-o-linked fucose on the reducing terminus on complex-type n-linked oligosaccharides. lentil lectin therefore provides an excellent example of a carbohydrate binding protein in which high-affinity interactions are dependent on the integrity of the oligosaccharide core structure. we report here the synthesis of the 1-n-glycyl beta-derivative of gal beta 4glcnac beta 2man alpha 6(gal beta 4glcnac beta 2man ... | 1992 | 1420189 |
| binding of thyrotropin to lentil lectin is unchanged by thyrotropin-releasing hormone administration in three patients with thyrotropin-producing pituitary adenomas. | glycoproteins have increased affinity for lentil lectin when fucose residues are bound to n-acetylglucosamine in the "core region" of their asparagine-linked oligosaccharides. in three patients with thyrotropin (tsh)-producing pituitary tumors, the proportion of serum tsh isoforms that bound to lentil (70.8% +/- 15%) was higher than that seen for tsh from normal persons (32.5 +/- 8%). unlike normal subjects, the concentration of tsh circulating in the tumor patients after acute administration of ... | 1992 | 1446658 |
| duck lymphocytes. vi. requirement for phagocytic and adherent cells in lymphocyte transformation. | preparations of duck (anas platyrhynchos) spleen and blood lymphocytes depleted of cells capable of phagocytosing carbonyl iron gave lower transformation responses to the mitogens phytohaemagglutinin (pha), concanavalin a (con a), bandeiraea simplicifolia seed lectin (bss), wheat germ agglutinin (wga), lentil lectin (ll) and phorbol ester (pma) than intact cell preparations. when cell populations were fractionated on the basis of their adherence to plastic, it was found that the adherent cells w ... | 1992 | 1455688 |
| ricinus communis agglutinin i reacting and non-reacting butyrylcholinesterase in human cerebrospinal fluid. | differences in glycosylation of acetylcholinesterase (ache) and butyrylcholinesterase (buche) in human brain, plasma and cerebrospinal fluid (csf) have been investigated by means of their interaction with agarose-immobilized lectins. most of the ache in brain and csf was associated to concanavalin a (con a), lens culinaris (lca) and triticum vulgaris (wga) agglutinins, but little activity was adsorbed to ricinus communis agglutinin i (rcai). brain, plasma and csf buche was almost fully bound to ... | 1992 | 1461569 |
| characterisation of a novel glycoprotein (avgp50) in the avian nervous system, with a monoclonal antibody. | a size fractionated lentil lectin-positive fraction derived from a deoxycholate extract of 1-day-old chick forebrain membranes was used to generate a series of monoclonal antibodies (mabs) against neural antigens. one of these, mabsa1.7 recognises a glycoprotein which is enriched in synaptic plasma membranes, designated avgp50. polyacrylamide gel electrophoresis and western blots show that avgp50 is comprised of at least two glycoforms, with m(r)s of 53 kda and 49 kda respectively. avgp50 is ner ... | 1992 | 1473276 |
| [a method for determining the presence of inverted repeats in the chloroplast genome of higher plants]. | a plasmid containing fragments of rp12 and rps19 genes from the chloroplast genome of arabidopsis thaliana was developed. the presence of inverted repeats in the chloroplast dna of a. thaliana and vicia sativa, and their absence from two species of fabaceae family (lathyrus sativus, lens esculenta) were shown with the help of this plasmid. | 1992 | 1473714 |
| mature domestic cat oocyte does not express a cortical granule-free domain. | a premature release of cortical granules (cg), found in the cortex of unfertilized oocytes, and the resulting formation of a cg-free domain (cgfd) over the metaphase ii spindle are associated with nuclear maturation in the hamster and mouse. the objectives of our study were to characterize and compare the distribution of cg in immature, in vitro-matured, and in vivo-matured domestic cat oocytes while determining if a cgfd is formed that may be useful as a marker for stage and normalcy of oocyte ... | 1992 | 1477197 |
| interaction of ache with lens culinaris agglutinin reveals differences in glycosylation of molecular forms in sarcoplasmic reticulum membrane subfractions. | fractionation of muscle microsomes rich in sarcoplasmic reticulum (sr) by isopicnic centrifugation yielded three types of membranes. heavy (hm), intermediate (im), and light membranes (lm), with isopicnic points of 38, 33, and 25% w/w sucrose, were rich in terminal cisternae/triads, longitudinal sr, and t-tubules, respectively. all membrane subfractions displayed acetylcholinesterase (ache) activity. about 60, 80, and 50% of total ache in hm, im, and lm was extracted with a tris-saline-triton bu ... | 1992 | 1484390 |
| fucosylation of glycoproteins begins in the rough endoplasmic reticulum of mouse active thyrotrophs. | our aim was to determine whether fucosylation of glycoproteins begins in the rough endoplasmic reticulum (rer) of active thyrotrophs. this would contrast with most cells studied, in which fucosylation generally is associated with the golgi apparatus. mouse thyrotropic tumor tissue was incubated with [35s]methionine for 2, 5, 7, 10, 30, and 90 minutes. tsh and free alpha-subunits were immunoprecipitated from cell lysates, and they displayed a time-dependent increase in affinity for lentil lectin ... | 1992 | 1493377 |
| interaction of dna with cationic liposomes: ability of transfecting lentil protoplasts. | the vesicle made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were multilamellar and rather homogeneous in shape as seen by transmission electron microscopy. upon addition of circular dna plasmids of different lengths to the liposomes, the formation of vesicle clusters around the dna filament was observed, with dimensions dictated by the ratio dna/lipid. these liposomes were able to transfect lentil (lens culinaris) protoplasts inside the cells two different reporter genes, chloramph ... | 1992 | 1510671 |
| bronchial asthma induced by chick pea and lentil. | allergic reactions to legumes through inhalation have rarely been described. we report the case of a 20-year-old man who experienced asthmatic attacks when exposed to the steam from cooking either chick pea or lentil. type i hypersensitivity to the antigens in these legumes was demonstrated by means of immediate skin reactivity, histamine release tests, rast and rast inhibition. specific bronchial challenges with the heated (75 degrees for 30 min) extracts of chick pea and lentil elicited isolat ... | 1992 | 1514671 |