| [examination and composition of some legume seeds (author's transl)]. | the chemical composition of some legume seeds is reviewed. it was found that soy (glycine soja), field beans (vicia faba), vicia sativa and lens culingris (red seeds) have the highest protein content. there were only minor differences in amino acid composition except for glutamic acid. the lysine content of legume seeds was high. lysine is the most limiting essential amino acid in cereal proteins, therefore cereal products can be improved nutritionally by addition of legume seed flour. examinati ... | 1977 | 560092 |
| studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels. | a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ... | 1978 | 563738 |
| additional precipitation reactions of lectins with human serum glycoproteins. | highly purified human serum glycoproteins were treated with neuraminidase and examined for their cross reaction with several lectins with anti-galactosyl specificity: beta-d-galactosyl structures are thought to be the main terminal sugar residues that become attached de novo after removal of neuraminic acid. the following lectins were tested: tridacnin from the bivalve clams tridacna maxima and tridacna gigas, the agglutinin from the sponge axinella polypoides, the lectin from the roach rutilus ... | 1978 | 564939 |
| isolation and characterization of rhizobitoxine mutants of bradyrhizobium japonicum. | to explore the role of rhizobitoxine in bradyrhizobium-legume symbiosis, 11 rhizobitoxine mutants of b. japonicum usda61 were isolated on the basis of their inability to synthesize the toxin in culture. each mutant is prototrophic and symbiotically effective on soybean, cowpea, siratro, and glycine soja. the rhizobitoxine mutants differ in their chlorosis phenotypes and rhizobitoxine production in planta. as expected, one group of mutant fail to make toxin in planta, resulting in the absence of ... | 1992 | 1317377 |
| molecular cloning and expression in photosynthetic bacteria of a soybean cdna coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. | carotenoids are orange, yellow, or red photo-protective pigments present in all plastids. the first carotenoid of the pathway is phytoene, a colorless compound that is converted into colored carotenoids through a series of desaturation reactions. genes coding for carotenoid desaturases have been cloned from microbes but not from plants. we report the cloning of a cdna for pds1, a soybean (glycine max) gene that, based on a complementation assay using the photosynthetic bacterium rhodobacter caps ... | 1991 | 1862081 |
| characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination. | th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ... | 1989 | 2617954 |
| electrophoretic study of alpha-d-galactosidases from seeds of glycine soja and vigna radiata possessing erythroagglutinating activity. | polyacrylamide gel electrophoresis in an acidic buffer system was used to study the electrophoretic behaviour of two forms of alpha-d-galactosidase from seeds of soy bean (glycine soja) and mung bean (vigna radiata). the interaction of the enzymes with saccharides was monitored by affinity electrophoresis; for the preparation of affinity gels, water-soluble o-glycosyl polyacrylamide copolymers and polysaccharides were used. alpha-d-galactosidases from both sources interact with immobilized alpha ... | 1988 | 2836454 |
| uneventful administration of plasma products in a recipient with t-activated red cells. | a patient with t-polyagglutinable red cells and a severe coagulopathy provided an opportunity to observe the results of plasma transfusion in the face of t-activation. the patient was a 52-year-old navajo indian with a perforated gall bladder and related sepsis due to clostridium perfringens. the gall bladder was removed surgically. postoperatively, he had severe thrombocytopenia, and prolonged partial thromboplastin and prothrombin times. the patient's red cells were agglutinated by arachis hyp ... | 1986 | 2869600 |
| evidence for plasmid- and chromosome-borne multiple nif genes in rhizobium fredii. | rhizobium fredii is a fast-growing rhizobium isolated from the primitive chinese soybean cultivar peking and from the wild soybean glycine soja. this rhizobium harbors nif genes on 150- to 200-megadalton plasmids. by passage on acridine orange plates, we obtained a mutant of r. fredii usda 206 cured of the 197-megadalton plasmid (usda 206c) which carries both nif and nod genes. this strain, however, has retained its symbiotic effectiveness. probing ecori digests of wild-type and cured plasmid dn ... | 1985 | 2992376 |
| haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family. | twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ... | 1988 | 3377987 |
| physical and chemical studies of a low-molecular-weight form of urease. | 1. a new form of enzymically active jack-bean [canavalia ensiformis (l.) dc] urease corresponding to an s(20,w) value of 11.8s and a molecular weight of 260000 was investigated. 2. conversion of 18s urease (ec 3.5.1.5) into the 12s form depends on both low protein concentration and ph. above ph5.3 urease exists in the 18s form and below ph4.8 in the 12s form; between these two ph values a 12s-18s rapid-equilibrium process is observed. 3. comparison of the properties of 18s and 12s urease indicat ... | 1968 | 5691753 |
| blood sugar and total cholesterol lowering effect of glycine soja (sieb and zucc.), mucuna pruriens (d.c.) and dolichos biflorus (linn.) seed diets in normal fasting albino rats. | | 1968 | 5732448 |
| organization and expression of leghaemoglobin genes. | leghaemoglobin genes in soybean (glycine max) are present as a moderately reiterated family of sequences. since there are identical restriction site patterns of these sequences in dna isolated from leaf, root, or nodule tissue, the data suggest that no major changes in the organization or methylation of leghaemoglobin genes occur during their induction. cloned soybean leghaemoglobin-cdna cross hybridized with rna from root nodules of kidney bean (phaseolus vulgaris), and to a lesser extent, of p ... | 1982 | 6282418 |
| isolation and expression of rhizobium japonicum cloned dna encoding an early soybean nodulation function. | a first visible step in the nodulation of legumes by rhizobium spp. is the deformation and curling of root hairs. we have identified and cloned dna sequences encoding this function from two strains of rhizobium japonicum (usda 122 and usda 110) with a weakly homologous probe from rhizobium meliloti. root hair curling encoded by the cloned dna fragments was examined on soybeans (glycine soja ) after conjugative transfer of these sequences in broad-host-range vectors to various bacterial genera. p ... | 1984 | 6327649 |
| mode of infection, nodulation specificity, and indigenous plasmids of 11 fast-growing rhizobium japonicum strains. | eleven fast-growing strains of rhizobium japonicum were characterized with respect to indigenous plasmids and abilities to infect (inf+) and nodulate (nod+) cowpea, siratro, wild soybean, and three commercial cultivars of soybean. all strains caused infection via infection threads in root hairs and consistently nodulated cowpea, siratro, and wild soybean in growth pouches. interactions with commercial cultivars of soybean were strikingly strain specific. some combinations were nod-, and infectio ... | 1984 | 6542099 |
| effect of raw legume diets on disaccharidase activity in the small intestine of chicks. | the effect of four raw legume diets: field beans (vicia faba) (rfb), navy beans (phaseolus vulgaris) (rnb), soybeans (glycine soja) (rsb) and bitter vetch (vicia ervilia) (rbv), on disaccharidase activities in chick small intestine have been studied. maltase and sucrase activities, which vary with age, were determined in 1 to 60 day old animals, rfb and rbv diets had no effect on maltase activity and only increased sucrase activity in 60 day old chicks. both maltase and sucrase activities decrea ... | 1980 | 7192009 |
| studies on lectins. li. the role of mn2+ in the activity of the soybean lectin. | in contradistinction to previous reports, the lectin of soybeans (glycine soja) has been shown to retain its erythroagglutinating activity after complete removal of manganese from its molecule. the applied demetallization procedures (dialysis against 0.1 m hcl or 1m acetic acid and against 0.1 m edta following by dialysis against 1 m acetic acid) had no effect on the stability of the lectin subunit structure, as proved by ultracentrifugation analysis or thin-layer chromatography on sephadex g-20 ... | 1981 | 7197555 |
| intestinal absorption of d-galactose and l-leucine and intestinal disaccharidase activities in growing chickens fed different raw legume diets. | a significant (p less than .01) impairment in the rate of growth, along with a significant (p less than .01) inhibition in the rate of in vivo intestinal absorption of d-galactose and l-leucine, and in the in vitro intestinal absorption of d-galactose, was found in growing chickens fed ad libitum over a 60-day period, diets containing the raw legumes vicia faba, glycine soja, vicia ervilia, and phaseolus vulgaris as the main source of protein. furthermore, a significant (p less than .01) reducti ... | 1981 | 7301750 |
| effect of raw legume diets on intestinal absorption of d-galactose by chick. | the effect of four raw legume diets on the intestinal absorption of d-galactose and oxygen consumption were studied in chick. field beans (vicia faba), soybeans (glycine soja), bitter vetch (vicia ervilia), and navy beans (phaseolus vulgaris), were used. the intestinal absorption was determined by both in vivo and in vitro techniques. in vivo, only navy beans and soybeans inhibit intestinal transport of d-galactose, while in vitro all the diets do. oxygen consumption by intestinal rings increase ... | 1980 | 7394275 |
| bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. | seventeen strains of extra-slowly growing (esg) soybean rhizobia isolated from root nodules of glycine soja and glycine max growing in five provinces (liaoning, heilongjiang, shanxi, hubei, and anhui) in the people's republic of china were compared with 48 reference strains belonging to the genera bradyrhizobium, rhizobium, and agrobacterium by performing a numerical analysis of 191 phenotypic features. our results showed that all of the esg strains examined clustered closely in the genus bradyr ... | 1995 | 7547289 |
| structural requirements of synthetic and natural product lipo-chitin oligosaccharides for induction of nodule primordia on glycine soja. | rhizobia synthesize a class of lipo-chitin oligosaccharides that induce root hair deformation and induce the initiation of nodule structures on legume roots. these lipo-chitin oligosaccharides are tetra- and penta-lipo-oligosaccharides of n-acetylglucosamine with an acyl substitution on the nonreducing end and are commonly known as nod factors. in this study, we demonstrate that synthetic analogs of natural product nod factors have the same biological activities. to determine structure-activity ... | 1995 | 7659753 |
| bradyrhizobium elkanii lipo-oligosaccharide signals induce complete nodule structures on glycine soja siebold et zucc. | | 1994 | 7764873 |
| the structures and biological activities of the lipo-oligosaccharide nodulation signals produced by type i and ii strains of bradyrhizobium japonicum. | bradyrhizobium japonicum produces lipo-oligosaccharide signal molecules that induce deformation of root hairs and meristematic activity on soybeans. b. japonicum usda135 (a type i strain) produces modified chitin pentasaccharide molecules with either a terminal n-c16:0- or n-c18:1-glucosamine with and without an o-acetyl group at c-6 and with 2-o-methylfucose linked to c-6 of the reducing n-acetylglucosamine. an additional molecule has n-c16:1-glucosamine and no o-acetyl group. all of these mole ... | 1993 | 8349712 |
| direct end labelling of telomeres. | a novel approach of direct end labelling of telomeres is presented. chromosome-sized, agarose-embedded dna was treated with t4 dna polymerase to remove protruding 3' end of telomeres and to generate single-stranded 5' ends. the dna was then labelled by the same enzyme in the presence of [alpha-32p]dgtp and cold datp and dttp. labelled yeast chromosomes separated by pulsed field gel electrophoresis maintained their integrity. digestion of yeast chromosomes separated in pulsed field gels with a re ... | 1993 | 8514152 |
| expression of the early nodulin, enod40, in soybean roots in response to various lipo-chitin signal molecules. | the lipo-chitin (lco) nodulation signal (nod signal) purified from bradyrhizobium japonicum induced nodule primordia on soybean (i.e. glycine soja) roots. these primordia were characterized by a bifurcated vascular connection, cortical cell division, and the accumulation of mrna of the early nodulin gene, enod40. a chemically synthesized lco identical in structure to the nod signal purified from b. japonicum cultures showed the same activity when inoculated on to soybean roots. surprisingly, syn ... | 1996 | 8758977 |
| nodz of bradyrhizobium extends the nodulation host range of rhizobium by adding a fucosyl residue to nodulation signals. | the nodulation genes of rhizobia are involved in the production of the lipo-chitin oligosaccharides (lco), which are signal molecules required for nodule formation. a mutation in nodz of bradyrhizobium japonicum results in the synthesis of nodulation signals lacking the wild-type 2-o-methylfucose residue at the reducting-terminal n-acetylglucosamine. this phenotype is correlated with a defective nodulation of siratro (macroptilium atropurpureum). here we show that transfer of nodz to rhizobium l ... | 1996 | 8858593 |
| aberrant nodulation response of vigna umbellata to a bradyrhizobium japonicum nodz mutant and nodulation signals. | the (brady)rhizobium nodulation gene products synthesize lipo-chitin oligosaccharide (lco) signal molecules that induce nodule primordia on legume roots. in spot inoculation assays with roots of vigna umbellata, bradyrhizobium elkanii lco and chemically synthesized lco induced aberrant nodule structures, similar to the activity of these lcos on glycine soja (soybean). lcos containing a pentameric chitin backbone and a reducing-end 2-o-methyl fucosyl moiety were active on v. umbellata. in contras ... | 1999 | 10494629 |
| differential expression of two soybean apyrases, one of which is an early nodulin. | two cdna clones were isolated from soybean (glycine soja) by polymerase chain reaction with primers designed to conserved motifs found in apyrases (nucleotide phosphohydrolase). the two cdnas are predicted to encode for two, distinct, apyrase proteins of approximately 50 kda (i.e., gs50) and 52 kda (i.e., gs52). phylogenetic analysis indicated that gs52 is orthologous to a family of apyrases recently suggested to play a role in legume nodulation. gs50 is paralogous to this family and, therefore, ... | 2000 | 11043467 |
| kinetics of nodule development in glycine soja. | nodule development in the interaction of glycine soja sieb. & zucc. pi468.397 with bradyrhizobium japonicum usda110 was studied by hypochlorite clearing and methylene blue staining. even the earliest stages of nodule development could be observed. the entire length of the primary root was examined up to 15 d postinoculation. markedly curled root hairs and the first cell divisions in the hypodermal layer (stage i) were observed 2 d postinoculation, and by 3 d cell division activity had spread to ... | 1993 | 12232007 |
| adaptation of cucumber mosaic virus soybean strains (ssvs) to cultivated and wild soybeans. | cucumber mosaic virus soybean strains formerly called soybean stunt virus (ssv) were inoculated onto 23 wild soybeans collected from four asian countries to investigate their infectivity in order to improve understanding of the co-evolution of ssvs and soybean. ssv inoculation resulted in systemic infection in most of the wild soybeans used. however, an ssv strain (ssv-in), which was isolated in indonesia, did not result in systemic infection of many of the wild soybeans distributed in southern ... | 2003 | 12835932 |
| specificity of glycine soja agglutinins. | | 1963 | 14103469 |
| purification of glysojanin, an antifungal protein, from the black soybean glycine soja. | a monomeric protein, with a molecular mass of 25 kda and an n-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean glycine soja. the protein, designated glysojanin, demonstrated potent antifungal activity against the fungi fusarium oxysporum and mycosphaerella arachidicola. it inhibited hiv-1 reverse transcriptase with an ic50 of 47 micromol/l, [methyl-3h]thymidine incorporation by mouse spleen cells with an ic50 of 175 micromol/l, and trans ... | 2003 | 14663505 |
| [effect of sinorhizobium fredii yc4 symbiotic plasmid amplification on nod factors and symbiotic n-fixation]. | sinorhizobium fredii yc4 can form nitrogen-fixing nodules on soybean (glycine max) and wild soybean (g. soja). it can produce unique lipochitooligosaccharide nod factors (lcos), in comparison with the other four strains of s. fredii. the constitution of lcos produced by yc4 contained more hydrophobic substitutions detected by thin-layer chromatography (tlc) of 14c-labeled nod factors. a spontaneous mutant termed ysc3 amplified in the symbiotic plasmid was isolated from yc4, which can produce mor ... | 2003 | 14733011 |
| [assessment of genetic diversity of wild soybean (glycine soja siebold et zucc.) in the far eastern region of russia]. | polymorphism of rapd markers was analyzed in the wild soybean populations from the far east region of russia. the level of rapd marker polymorphism was significantly higher in the wild than in the cultivated soybean. the results obtained suggest active development of genetically different groups of wild soybean. geographically isolated subpopulations showed maximum distance from the main population of wild soybean. | 2004 | 15065430 |
| resistance of glycine species and various cultivated legumes to the soybean aphid (homoptera: aphididae). | the soybean aphid, aphis glycines matsumura, is a new pest of soybean, glycine max (l.) merr., in north america. it has become widespread on soybean in north america since it was first identified in the midwest in 2000. species of rhamnus l. (buckthorn) are the primary hosts of a. glycines, and soybean is known as a secondary host. there is limited information about the secondary host range of a. glycines. aphid colonization on various legume hosts was compared in choice experiments. aphid colon ... | 2004 | 15279293 |
| selection on glycine beta-1,3-endoglucanase genes differentially inhibited by a phytophthora glucanase inhibitor protein. | plant endo-beta-1,3-glucanases (egases) degrade the cell wall polysaccharides of attacking pathogens and release elicitors of additional plant defenses. isozymes egasea and egaseb of soybean differ in susceptibility to a glucanase inhibitor protein (gip1) produced by phytophthora sojae, a major soybean pathogen. egasea, the major elicitor-releasing isozyme, is a high-affinity ligand for gip1, which completely inhibits it, whereas egaseb is unaffected by gip1. we tested for departures from neutra ... | 2005 | 15545660 |
| in vitro antimycotic activity of some plant extracts towards yeast and yeast-like strains. | as part of screening aimed at the selection of novel antimycotic compounds of vegetable origin, leaf extracts of camellia sinensis l., cupressus sempervirens l. and pistacia lentiscus l. and the seed extract of glycine soja sieb. et zucc. were tested against yeast and yeast-like species implicated in human mycoses. of the extracts only those of c. sinensis (obtained from a commercial preparation of green tea) exhibited broad activity towards candida glabrata, clavispora lusitatiae, cryptococcus ... | 2005 | 15798996 |
| genetic divergence between north american ancestral soybean lines and introductions with resistance to soybean cyst nematode revealed by chloroplast haplotype. | domesticated soybean [glycine max (l.) merr.] is a major crop with an established ancestral relationship to wild soybean (glycine soja sieb. & zucc.) native to asia. soybean genetic diversity can be assessed at different levels by identification of polymorphic alleles at genetic loci, in either the plastid or nuclear genomes. the objective of this study was to evaluate genetic diversity based on chloroplast haplotypes for soybean genotypes present in the usda germplasm resource collection. share ... | 2005 | 15947084 |
| fast-growing rhizobium japonicum that effectively nodulates several commercial glycine max l. merrill cultivars. | several isolates of fast-growing rhizobium japonicum that nodulate the wild soybean glycine soja have been recently described (keyser et al., science 215:1631-1632, 1982). we demonstrate that one of these isolates, designated prc 440 or usda 191, has a wider host range than that previously reported and is able to nodulate several commercial glycine max cultivars as effectively as does slow-growing r. japonicum 61a76. electron microscopic examination revealed no obvious differences between strain ... | 1984 | 16346594 |
| influence of glycine spp. on competitiveness of bradyrhizobium japonicum and rhizobium fredii. | the displacement of indigenous bradyrhizobium japonicum in soybean nodules with more effective strains offers the possibility of enhanced n(2) fixation in soybean (glycine max (l.) merr.). our objective was to determine whether the wild soybean (g. soja sieb. & zucc.) genotype pi 468397 would cause reduced competitiveness of important indigenous b. japonicum strains usda 31, 76, and 123 and thereby permit nodulation by rhizobium fredii, the fast-growing microsymbiont of soybean. in an initial ex ... | 1988 | 16347589 |
| host recognition in the rhizobium-soybean symbiosis. | polar binding of rhizobium japonicum to roots and root hairs of glycine soja (l.) sieb. and zucc. is specifically inhibited by d-galactose and n-acetyl-d-galactosamine, haptens of glycine max seed lectin. a protein, immunologically cross-reactive with the g. max seed lectin, is present in g. soja seed extracts. peptide mapping of the purified g. max and g. soja lectins indicates that the two are similar in structure. soybean lectin can be localized on the surface of both g. max and g. soja roots ... | 1980 | 16661488 |
| genotypic variability of soybean response to agrobacterium strains harboring the ti or ri plasmids. | twenty four diverse cultivars of soybean (glycine max [l.] merrill) and three lines of its annual wild progenitor glycine soja sieb and zucc. were tested for their response to agrobacterium strains harboring either the ti (tumor-inducing) plasmid (pti) from agrobacterium tumefaciens or the ri (root-inducing) plasmid (pri) from agrobacterium rhizogenes following uniform wounding and inoculation. based upon gall weight at 8 weeks postinfection, three g. max cultivars (biloxi, jupiter, and peking) ... | 1985 | 16664035 |
| two indirect methods for detecting ureide synthesis by nodulated legumes. | two methods were developed for the detection of altered ureide metabolism in legume nodules. both techniques are based on the positive correlation between the presence of high xanthine dehydrogenase (ec 1.2.1.37) specific activity in nodules and the ability of those nodules to produce the ureides, allantoin and allantoic acid. in the first method, nodulated legumes are treated for 2 weeks with a soil drench of allopurinol. after allopurinol treatment, leaves of n(2)-fed, ureide-producing legumes ... | 1986 | 16664857 |
| infection of soybean by cucumber mosaic virus as determined by viral movement protein. | to characterize the host range determinant of the soybean strain of cucumber mosaic virus (cmv) we analyzed a series of pseudorecombinants and chimeric viruses between infectious transcripts from two soybean strains (cmv-sc and cmv-sd) and an ordinary strain (cmv-y). cmv-y could not infect soybeans, even locally. systemic infection of the two soybean-adapted soybean isolates on soybean plants mapped to rna3. chimeric rna3s from between cmv-sc and cmv-y, and chimeric rna3s from between cmv-sc and ... | 2007 | 17006598 |
| genetic spatial clustering: significant implications for conservation of wild soybean (glycine soja: fabaceae). | knowledge of spatial patterns of genetic variation within populations of wild relative species has significant implications with respect to sampling strategies for ex situ and in situ conservation. to study spatial genetic structure of wild soybean (glycine soja sieb. et zucc.) at the fine scale, three natural populations in northern china were analyzed using inter-simple sequence repeat (issr) fingerprints for estimating kinship coefficients. a regression analysis of kinship coefficients agains ... | 2006 | 17028939 |
| qtl associated with horizontal resistance to soybean cyst nematode in glycine soja pi464925b. | soybean cyst nematode (heterodera glycines ichinohe; scn) is the primary disease responsible for yield loss of soybean [glycine max (l.) merr.]. resistant cultivars are an effective management tool; however, the sources currently available have common resistant genes. glycine soja sieb. and zucc., the wild ancestor of domesticated soybean, represents a diverse germplasm pool with known scn resistance. the objectives of this research were to: (1) determine the genetic variation and inheritance of ... | 2007 | 17119912 |
| cloning and functional characterization of an o-acetylserine(thiol)lyase-encoding gene in wild soybean (glycine soja). | the terminal step of soybean cysteine synthesis is catalyzed by o-acetylserine(thiol)lyase (oas-tl, ec 2.5.1.47). in this study, we isolated and characterized an oas-tl gene from a wild soybean material (designated as gsoas-tl1). gsoas-tl1 cdna sequence showed strict conservation at both nucleotide and amino acid levels compared with that from cultivated soybean. genomic structure analysis of gsoas-tl1 indicated that it contained 10 exons and 9 introns in the coding region with conserved exon si ... | 2008 | 17611817 |
| [investigation of genetically modified soybean biosafety in the center of the origin and diversity of russian far east]. | wild soybean (glycine soja sieb. & zucc.) is the nearest relative of a soybean crop (glycine max (l.) merr.). study of population genetic structure of wild-growing relatives ofgenetically modified (gm) plants in the centers of their origin is one of the main procedures before introduction of gm crops in these areas. we have studied genetic variability of nine wild growing soya populations of primorye territory using rapd analysis. the level of g. soja genetic variability was considerably higher ... | 2007 | 17649627 |
| plant community succession in modern yellow river delta, china. | data were collected in different successional stages using a simultaneous sampling method and analyzed through quantitative classification method. three large groups and 12 classes were made to represent the community patterns of three succession stages and 12 succession communities. the succession series of plant community in the study area was as follows: saline bare land-->community suaeda salsa-->community tamarix chinensis-->grassland. succession degree and succession process of 12 successi ... | 2007 | 17657854 |
| an alloantibody to a high-prevalence mns antigen in a person with a gp.jl/mk phenotype. | the low-prevalence mns blood group antigentsen is located at the junction of glycophorin a (gpa) to glycophorin b (gpb) in several hybrid glycophorin molecules. extremely rare people have rbcs with a double dose of the tsen antigen and have made an antibody to a high-prevalence mns antigen. we report the first patient who is heterozygous for gyp.jl and mk. during prenatal tests,an alloantibody to a high-prevalence antigen was detected in the serum of a 21-year-old hispanic woman. the antibody de ... | 2007 | 18284304 |
| study of the refirming effect of a plant complex. | loss of skin elasticity is one of the main problems of ageing. this is a mechanical property influenced by elastin, a protein in the dermis which, together with collagen and glycosaminoglycans, makes up the connective tissue. this tissue is affected by a large number of events (such as cutaneous ageing, pregnancy, slimming processes and cellulitis) which eventually cause it to change. at the same time, the metabolism of the proteins of the connective tissue decreases and there is an ever greater ... | 1998 | 18505506 |
| allelic differentiation of kunitz trypsin inhibitor in wild soybean (glycine soja). | soybean kunitz trypsin inhibitor (skti) has several polymorphic types, which are controlled by co-dominant multiple alleles at a single locus. of these types, tia and tib are predominant types, and there are nine differences in amino acids between tia and tib. recently, an intermediate transitional type (tibi5) between them was detected. however, other transitional types have not been detected despite surveys of many cultivated and wild soybeans. one of the reasons why other transitional variant ... | 2008 | 18512041 |
| affinity purification of trypsin inhibitor with anti-aspergillus flavus activity from cultivated and wild soybean. | trypsin inhibitors (ti) from wild-type soybean (glycine soya) (wbti) and domesticated soybean (glycine max) (sbti) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. the sbti/wbti purification fold by affinity chromatography was 718- and 279-fold, with the activity recovery of 62% and 59%, respectively. it was found that sbti and wbti exerted a strong inhibition of aspergillus. flavus growth, with ic(50) of 1.6 and 1.0 micromol/l. this growth inh ... | 2009 | 18830687 |
| gs52 ecto-apyrase plays a critical role during soybean nodulation. | apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphates and nucleoside diphosphates to nucleoside monophosphates and orthophosphates. gs52, a soybean (glycine soja) ecto-apyrase, was previously shown to be induced very early in response to inoculation with the symbiotic bacterium bradyrhizobium japonicum. overexpression of the gs52 ecto-apyrase in lotus japonicus increased the level of rhizobial infection and enhanced nodulation. these data suggest a ... | 2009 | 19036836 |
| genetic diversity and peculiarity of annual wild soybean (g. soja sieb. et zucc.) from various eco-regions in china. | annual wild soybean (glycine soja sieb. et zucc.) is believed to be a potential gene source for future soybean improvement in coping with the world climate change for food security. to evaluate the wild soybean genetic diversity and differentiation, we analyzed allelic profiles at 60 simple-sequence repeat (ssr) loci and variation of eight morph-biological traits of a representative sample with 196 accessions from the natural growing area in china. for comparison, a representative sample with 20 ... | 2009 | 19449177 |
| inheritance of salt tolerance in wild soybean (glycine soja sieb. and zucc.) accession pi483463. | tolerant soybean (glycine max [l.] merr.) cultivars aid in reducing salt damage in problem fields. new genes are important to reduce losses from salt injury. objectives of this study were to determine inheritance of salt tolerance in wild soybean (glycine soja sieb. and zucc.) pi483463 and to test allelism of tolerance genes from genotypes pi483463 and s-100, a common ancestor of southern in us cultivars. tolerant (t) pi483463 was crossed to sensitive (s) cultivar hutcheson to study inheritance. ... | 2009 | 19451208 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| biological safety of liposome-fullerene consisting of hydrogenated lecithin, glycine soja sterols, and fullerene-c60 upon photocytotoxicity and bacterial reverse mutagenicity. | various water-soluble derivatives of fullerene-c60 (c60) have been developed as detoxifiers for reactive oxygen species (ros), whereas c60 incorporated in liposome (lpsm) has not been reported yet. we prepared the liposome-fullerene (0.2% aqueous phase, lpsm-flln) which was composed of hydrogenated lecithin, glycine soja (soybean) sterols, and c60 in the weight ratio of 89.7:10:0.3, then examined the photocytotoxicity and bacterial reverse mutagenicity, as comparing with the lpsm containing no c ... | 2009 | 19482914 |
| effects of nacl stress on antioxidative enzymes of glycine soja sieb. | the activity of anti-oxidant enzymes (superoxide dismutase (sod), peroxidase (pod), catalase (cat) and parameters of oxidative stress malondialdehyde (mda) of shoots were investigated in s. sieb naturally salt-resistant halophyte. the seedlings of s. sieb were treated with varying (0, 80, 160 and 240 mm) nacl stress. the results showed that nacl played an important role in growth of s. sieb. it made obviously promotion of certain nacl concentration to growth of s. sieb, the seeflings of s. sieb ... | 2009 | 19580000 |
| fullerene-c60/liposome complex: defensive effects against uva-induced damages in skin structure, nucleus and collagen type i/iv fibrils, and the permeability into human skin tissue. | we previously reported biological safety of fullerene-c60 (c60) incorporated in liposome consisting of hydrogenated lecithin and glycine soja sterol, as liposome-fullerene (0.5% aqueous phase; a particle size, 76nm; lpsm-flln), and its cytoprotective activity against uva. in the present study, lpsm-flln was administered on the surface of three-dimensional human skin tissue model, rinsed out before each uva-irradiation at 4 j/cm(2), and thereafter added again, followed by 19-cycle-repetition for ... | 2010 | 20036139 |
| [genetic variation of snp loci based on candidate gene for resistance to soybean cyst nematode]. | for clarifying the difference of genetic diversity and linkage disequilibrium (ld) level between cultivated (glycine max (l.) merr.) and annual wild soybean (glycine soja sieb. & zucc.), genetic variation pattern of 8 snp loci developed from soybean cyst nematode resistance candidate genes rhg1 and rhg4 in soybean germplasm were analyzed. the results indicated that g. max population, consisted of cultivated soybean mini-core collection and modern cultivars, had a higher ld levels (r2 value is 0. ... | 2009 | 20042394 |
| identification of a major qtl allele from wild soybean (glycine soja sieb. & zucc.) for increasing alkaline salt tolerance in soybean. | salt-affected soils are generally classified into two main categories, sodic (alkaline) and saline. our previous studies showed that the wild soybean accession jws156-1 (glycine soja) from the kinki area of japan was tolerant to nacl salt, and the quantitative trait locus (qtl) for nacl salt tolerance was located on soybean linkage group n (chromosome 3). further investigation revealed that the wild soybean accession jws156-1 also had a higher tolerance to alkaline salt stress. in the present st ... | 2010 | 20204319 |
| over-expression of a glutathione s-transferase gene, gsgst, from wild soybean (glycine soja) enhances drought and salt tolerance in transgenic tobacco. | glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. we constructed a cdna library from g. soja seedlings treated with nacl and isolated a glutathione s-transferase gene (gsgst: gq265911) from the library. the cdna encoding gsgst contains an open reading frame of 660 bp and the predicted protein belongs to the tau class of gst family proteins. tobacco plants over-expressing the gsgst gene showed sixfold higher gst activity than wi ... | 2010 | 20383560 |
| artificial selection for determinate growth habit in soybean. | determinacy is an agronomically important trait associated with the domestication in soybean (glycine max). most soybean cultivars are classifiable into indeterminate and determinate growth habit, whereas glycine soja, the wild progenitor of soybean, is indeterminate. indeterminate (dt1/dt1) and determinate (dt1/dt1) genotypes, when mated, produce progeny that segregate in a monogenic pattern. here, we show evidence that dt1 is a homolog (designated as gmtfl1) of arabidopsis terminal flower 1 (t ... | 2010 | 20421496 |
| comparison of genetic diversity between canadian adapted genotypes and exotic germplasm of soybean. | soybean (glycine max (l.) merr.) was domesticated in china and the greatest genetic diversity for this species is found in asia. in contrast, in north america, soybean cultivars trace back to a small number of plant introductions from asia and genetic diversity is typically quite limited. the purpose of this work was to measure and compare the genetic diversity in two sets of soybean lines. the first set (termed "local") was composed of 100 lines used in a private breeding program in quebec. the ... | 2010 | 20616865 |
| ensifer (sinorhizobium) sojae sp. nov., isolated from root nodules of glycine max grown in saline-alkaline soils in hebei province of china. | thirteen bacterial strains isolated from root nodules of soybean grown in saline-alkaline soils in the chinese province of hebei were identified as a unique group in genus ensifer (sinorhizobium) based upon box-pcr patterns, sequencing analyses of 16s rrna and housekeeping genes, and dna-dna hybridization. phenotypically, positive acid production and negative reduction in litmus milk and sensitivity to 50 µg ml(1) of amplicillin, as well as some other features could differentiate the novel group ... | 2010 | 20851917 |
| genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in different ecoregions of china. | a total of 99 bacterial isolates that originated from root nodules of glycine soja were characterized with restriction analyses of amplified 16s ribosomal dna and 16s-23s rdna intergenic spacers (its), and sequence analyses of 16s rrna, rpob, atpd, reca and nodc genes. when tested for nodulation of g. soja, 72 of the isolates were effective symbionts, and these belonged to five species: bradyrhizobium japonicum, bradyrhizobium elkanii, bradyrhizobium yuanmingense, bradyrhizobium liaoningense and ... | 2011 | 21303397 |
| fullerene-c60 incorporated in liposome exerts persistent hydroxyl radical-scavenging activity and cytoprotection in uva/b-irradiated keratinocytes. | the aim of this study is to examine antioxidant activity of fullerene-c60 (c60) incorporated in liposome (lpsmflln, a diameter of 75.6 nm). lpsmflln is water-soluble, and composed of hydrogenated lecithin of 89.7%, glycine soja sterol of 10% and c60 of 0.3%. hydroxyl radicals (*oh), generated from uva- or uvb-irradiated h2o2, were scavenged by lpsmflln but not by c60-lacking lpsm as assessed by esr, showing that the active principle is c60 as scanty as 1/415 weight versus lpsmflln; the *oh amoun ... | 2011 | 21780373 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| The ß-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the a-subunit genes. | ß-Conglycinin, a major seed protein in soybean, is composed of a, a', and ß subunits sharing a high homology among them. Despite its many health benefits, ß-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the a, a', and ß subunits also contain major allergens. A wild soybean (Glycine soja Sieb et Zucc.) line, 'QT2', lacks all of the ß-conglycinin subunits, and the deficien ... | 2011 | 22193750 |
| bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (glycine max l.) nodules in northern (huang-huai-hai) plain of china. | in a survey of biodiversity and biogeography of rhizobia associated with soybean (glycine max l.) in different sites of northern (huang-huai-hai) plain of china, ten strains were defined as a novel genomic species in the genus of bradyrhizobium. they were distinguished from defined species in rflp (restriction fragment length polymorphism) of 16s rrna gene and intergenic spacer (igs) between 16s and 23s rrna genes. in box-pcr, these strains presented two patterns sharing 94% similarities, demons ... | 2011 | 22003042 |
| genome-wide association and genomic prediction identifies associated loci and predicts the sensitivity of tobacco ringspot virus in soybean plant introductions. | genome-wide association study (gwas) is a useful tool for detecting and characterizing traits of interest including those associated with disease resistance in soybean. the availability of 50,000 single nucleotide polymorphism (snp) markers (soysnp50k iselect beadchip; www.soybase.org ) on 19,652 soybean and wild soybean plant introductions (pis) in the usda soybean germplasm collection allows for fast and robust identification of loci associated with a desired phenotype. by using a genome-wide ... | 2016 | 26924079 |
| uncovering the salt response of soybean by unraveling its wild and cultivated functional genomes using tag sequencing. | soil salinity has very adverse effects on growth and yield of crop plants. several salt tolerant wild accessions and cultivars are reported in soybean. functional genomes of salt tolerant glycine soja and a salt sensitive genotype of glycine max were investigated to understand the mechanism of salt tolerance in soybean. for this purpose, four libraries were constructed for tag sequencing on illumina platform. we identify around 490 salt responsive genes which included a number of transcription f ... | 2012 | 23209559 |
| the national bioresource project (nbrp) lotus and glycine in japan. | the objective of the national bioresource project (nbrp) in japan is to collect, conserve and distribute biological materials for life sciences research. the project consists of twenty-eight bioresources, including animal, plant, microorganism and dna resources. nbrp lotus and glycine aims to support the development of legume research through the collection, conservation, and distribution of these bioresources. lotus japonicus is a perennial legume that grows naturally throughout japan and is wi ... | 2012 | 23136485 |
| identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing. | using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple qtls relevant to traits of interest in agriculture ... | 2014 | 25004933 |
| soybean gmdrebl increases lipid content in seeds of transgenic arabidopsis. | a dreb-type transcription factor gene gmdrebl has been characterized for its functions in oil accumulation in seeds. the gene is specifically expressed in soybean seeds. the gmdrebl is localized in nucleus and has transcriptional activation ability. overexpression of gmdrebl increased the fatty acid content in the seeds of transgenic arabidopsis plants. gmdrebl can bind to the promoter region of wri1 to activate its expression. several other genes in the fatty acid biosynthesis pathway were also ... | 2016 | 27694917 |
| the nuclear protein gmbzip110 has transcription activation activity and plays important roles in the response to salinity stress in soybean. | plant basic-leucine zipper (bzip) transcription factors play important roles in many biological processes and are involved in the regulation of salt stress tolerance. previously, our lab generated digital gene expression profiling (dgep) data to identify differentially expressed genes in a salt-tolerant genotype of glycine soja (stgogs) and a salt-sensitive genotype of glycine max (ssgogm). this dgep data revealed that the expression (log2 ratio) of gmbzip110 was up-regulated 2.76-fold and 3.38- ... | 2016 | 26837841 |
| bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in canada. | sixteen strains of symbiotic bacteria from root nodules of glycine max grown in ottawa, canada, were previously characterized and placed in a novel group within the genus bradyrhizobium. to verify their taxonomic status, these strains were further characterized using a polyphasic approach. all strains possessed identical 16s rrna gene sequences that were 99.79 % similar to the closest relative, bradyrhizobium liaoningense lmg 18230(t). phylogenetic analysis of concatenated atpd, glnii, reca, gyr ... | 2014 | 24969302 |
| computational identification and characterization of novel genes from legumes. | the fabaceae, the third largest family of plants and the source of many crops, has been the target of many genomic studies. currently, only the grasses surpass the legumes for the number of publicly available expressed sequence tags (ests). the quantity of sequences from diverse plants enables the use of computational approaches to identify novel genes in specific taxa. we used blast algorithms to compare unigene sets from medicago truncatula, lotus japonicus, and soybean (glycine max and glycin ... | 2004 | 15266052 |
| transformation by agrobacterium rhizogenes and regeneration of transgenic shoots of the wild soybean glycine argyrea. | glycine argyrea accession g1420 was evaluated for its response to inoculation with agrobacterium rhizogenes strains lba9402 and a4t, carrying wild type ri plasmids, and by strains r1601 and a4tiii with engineered plasmids. hypocotyls from young seedlings were the most responsive in producing roots at inoculation sites. root production was also dependent on bacterial concentration. excised, cultured roots produced green nodular callus which regenerated shoots on sc2 medium containing 1.1 mg l(-1) ... | 1991 | 24221492 |
| distribution and diversity of rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in northwest china. | a total of 155 nodule isolates that originated from seven sites in northwest china were characterized by pcr-rflp of the 16s rrna gene and sequence analysis of multiple core genes (16s rrna, reca, atpd, and glnii) in order to investigate the diversity and biogeography of glycine soja-nodulating rhizobia. among the isolates, 80 were ensifer fredii, 19 were ensifer morelense, 49 were rhizobium radiobacter, and 7 were putative novel rhizobium species. the phylogenies of e. fredii and e. morelense i ... | 2014 | 25052953 |
| a putative soybean gmssos1 confers enhanced salt tolerance to transgenic arabidopsis sos1-1 mutant. | the cdna of gmssos1, a putative plasma membrane na(+)/h(+) antiporter gene isolated from glycine max, glycine soja, and their hybrid, was constructed into plant expression vector pcambia 1300 and then transformed with agrobacterium tumefaciens under the control of camv 35s promoter to arabidopsis thaliana wild-type (wt) and mutant (atsos1-1) plants. by hygromycin resistance detection and pcr analysis, transgenic plants (wt35s:gmssos1 and atsos1-1 35s:gmssos1) were obtained. seed germination, see ... | 2015 | 24934653 |
| [endophytic bacterial diversity of wild soybean (glycine soja) varieties with different resistance to soybean cyst nematode (heterodera glycines)]. | the aim of this study was to investigate endophytic bacterial diversity of wild soybean varieties with different resistance to soybean cyst nematode(heterodera glycines) , for deciphering the interactions of soybean cyst nematode with endophytic bacteria. | 2014 | 25345025 |
| overexpression of gmcam4 in soybean enhances resistance to pathogens and tolerance to salt stress. | plant diseases inflict heavy losses on soybean yield, necessitating an understanding of the molecular mechanisms underlying biotic/abiotic stress responses. ca(2) (+) is an important universal messenger, and protein sensors, prominently calmodulins (cams), recognize cellular changes in ca(2) (+) in response to diverse signals. because the development of stable transgenic soybeans is laborious and time consuming, we used the bean pod mottle virus (bpmv)-based vector for rapid and efficient protei ... | 2014 | 24118726 |
| [influence of bean yellow mosaic virus on metabolism of photosynthetic pigments, proteins and carbohydrates in glycine soja l]. | this paper presents data on bymv effects on some physiological processes of glycine soja l. cultivated in the right-bank forest-steppe regions. pigment content (chlorophyll a, b and carotenoids), soluble proteins and water soluble carbohydrates were estimated and, as has been shown, are subjected to significant changes as compared with control plants, namely: a decrease in the content of chlorophyll a, b and carotenoids was 64%, 53% and 36% compared with the control plants. the significant incre ... | 2014 | 24800515 |
| molecular and biological characterization of an isolate of cucumber mosaic virus from glycine soja by generating its infectious full-genome cdna clones. | molecular and biological characteristics of an isolate of cucumber mosaic virus (cmv) from glycine soja (wild soybean), named as cmv-209, was examined in this study. comparison of nucleotide sequences and phylogenetic analyses of cmv-209 with the other cmv strains revealed that cmv-209 belonged to cmv subgroup i. however, cmv-209 showed some genetic distance from the cmv strains assigned to subgroup ia or subgroup ib. infectious full-genome cdna clones of cmv-209 were generated under the control ... | 2014 | 25288998 |
| pseudorecombination between two distinct strains of cucumber mosaic virus results in enhancement of symptom severity. | recently, a cucumber mosaic virus (cmv) strain, named as cmv-209, was isolated from glycine soja. in this study, symptom expression of cmv-209 was analyzed in detail in nicotiana benthamiana by comparing with that of cmv-fny, which is a representative strain of cmv. using infectious cdna clones of cmv strains 209 and fny, symptom expression of various pseudorecombinants between these two strains were examined in the early and late infection stages. in the early infection stage, the pseudorecombi ... | 2014 | 25289019 |
| genome-wide association study of resistance to soybean cyst nematode (heterodera glycines) hg type 2.5.7 in wild soybean (glycine soja). | soybean cyst nematode (scn) is the most destructive soybean pest worldwide. host plant resistance is the most environmentally friendly and cost-effective way of mitigating scn damage to soybeans. however, overuse of the resistant soybean [glycine max (l.) merr.] cultivars from limited genetic resources has resulted in scn race shifts in many soybean-growing areas. thus, exploration of novel sources of scn resistance and dissection of the genetic basis are urgently needed. in this study, we scree ... | 2016 | 27582748 |
| soybean cyst nematode resistance emerged via artificial selection of duplicated serine hydroxymethyltransferase genes. | a major soybean (forrest cultivar) quantitative trait locus (qtl) gene, rhg4, which controls resistance to soybean cyst nematodes (scn), encodes the enzyme serine hydroxylmethyltransferase (shmt). the resistant allele possesses two critical missense mutations (p130r and n358y) compared to that of the sensitive allele, rhg4. to understand the evolutionary history of this gene, sequences of 117 shmt family members from 18 representative plant species were used to reconstruct their phylogeny. accor ... | 2016 | 27458476 |
| genetic diversity of rhg1 and rhg4 loci in wild soybeans resistant to soybean cyst nematode race 3. | over-utilization of germplasms that are resistant to the soybean cyst nematode (scn) in soybean breeding programs can lead to genetic vulnerability in resistant cultivars. resistant wild soybean (glycine soja) is considered an invaluable gene source for increasing the genetic diversity of scn resistance. in this study, we genotyped 23 g. soja accessions that are resistant to scn race 3 for polymorphisms in the resistance genes, rhg1, rhg4, and shmt, and investigated their genetic relationship wi ... | 2016 | 27323148 |
| dna sequence polymorphism of the rhg4 candidate gene conferring resistance to soybean cyst nematode in chinese domesticated and wild soybeans. | rhg4 is one of the major resistant genes conferring resistance to soybean cyst nematode races 1, 3 and 4. in order to better understand its sequence diversity among different chinese soybean populations and the impact of human activities on it, we designed 5 primer sets based on its sequence deposited in genbank (genbank accession no. af506518) to obtain the rhg4 sequence from 104 chinese cultivated and wild soybean genotypes, and then analyzed the dna sequence polymorphism in different chinese ... | 2012 | 22924021 |
| functional analysis of flower development related gene gslfy from glycine soja. | leafy/floricaula (lfy/flo) is a family of plant-specific transcription factors, which plays an important role(s) in the regulation of floral organ formation and development. so far, lfy regulation on floral development in wild soybean has not been reported in the literature. in this study, the lfy gene, gslfy, has been isolated from glycine soja, and characterized with molecular and transgenic techniques. the cdna for gslfy gene is 1224 bp in length and contains an open reading frame encoding a ... | 2017 | 28115306 |
| a 14-3-3 family protein from wild soybean (glycine soja) regulates aba sensitivity in arabidopsis. | it is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. by conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in aba responses. hence, in this study, based on the latest glycine max genome on phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, ... | 2015 | 26717241 |
| a novel glycine soja cysteine proteinase inhibitor gscpi14, interacting with the calcium/calmodulin-binding receptor-like kinase gscbrlk, regulated plant tolerance to alkali stress. | it has been well demonstrated that cystatins regulated plant stress tolerance through inhibiting the cysteine proteinase activity under environmental stress. however, there was limited information about the role of cystatins in plant alkali stress response, especially in wild soybean. here, in this study, we focused on the biological characterization of a novel glycine soja cystatin protein gscpi14, which interacted with the calcium/calmodulin-binding receptor-like kinase gscbrlk and positively ... | 2014 | 24407891 |
| gslrpk, a novel cold-activated leucine-rich repeat receptor-like protein kinase from glycine soja, is a positive regulator to cold stress tolerance. | plant lrr-rlks serve as protein interaction platforms, and as regulatory modules of protein activation. here, we report the isolation of a novel plant-specific lrr-rlk from glycine soja (termed gslrpk) by differential screening. gslrpk expression was cold-inducible and shows ser/thr protein kinase activity. subcellular localization studies using gfp fusion protein indicated that gslrpk is localized in the plasma membrane. real-time pcr analysis indicated that temperature, salt, drought, and aba ... | 2014 | 24388511 |
| [identification of calcium/calmodulin-binding receptor-like kinase gscbrlk-interactive proteins using yeast two-hybrid system]. | gscbrlk (calcium/calmodulin-binding receptor-like kinase from glycine soja) links aba (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. in order to study the molecular mechanisms of gscblrk, the salt-treated glycine soja cdna library was screened with pbt3-ste-cbrlk as bait plasmid using yeast two hybrid system. two positive clones (snare and 14-3-3 protein) were identified by constructing cdna library of wild soybean under salt treatment, membrane system yeast two hybr ... | 2013 | 23575546 |
| ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (glycine max). | two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus ensifer based on 16s rrna gene comparisons. they were also verified as a separated group by the concatenated sequence analyses of reca, atpd and glnii (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ani) between the whole genome sequence of the representative strain ccbau 251167(t) and those of th ... | 2017 | 28209394 |
| ensifer glycinis sp. nov., a rhizobial species associated with species of the genus glycine. | rhizobial strains from root nodules of astragalus mongholicus and soybean (glycine max) were characterized phylogenetically as members of the genus ensifer (formerly named sinorhizobium), based on 16s rrna gene sequence comparisons. results based upon concatenated sequence analysis of three housekeeping genes (reca, atpd and glnii, ≤ 93.8 % similarities to known species) and average nucleotide identity (ani) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to ensifer fr ... | 2016 | 27125987 |
| improvement in nitrogen fixation capacity could be part of the domestication process in soybean. | biological nitrogen fixation (bnf) in soybeans is a complex process involving the interplay between the plant host and the symbiotic rhizobia. as nitrogen supply has a crucial role in growth and development, higher nitrogen fixation capacity would be important to achieve bigger plants and larger seeds, which were important selection criteria during plant domestication by humans. to test this hypothesis, we monitored the nitrogen fixation-related performance in 31 cultivated and 17 wild soybeans ... | 2016 | 27118154 |
| complete nucleotide sequences of seven soybean mosaic viruses (smv), isolated from wild soybeans (glycine soja) in china. | soybean mosaic virus (smv) is a devastating plant virus classified in the family potyviridae, and known to infect cultivated soybeans (glycine max). in this study, seven new smvs were isolated from wild soybean samples and analyzed by whole-genome sequencing. an updated smv phylogeny was built with the seven new and 83 known smv genomic sequences. results showed that three northeastern smv isolates were distributed in clade iii and iv, while four southern smvs were grouped together in clade ii a ... | 2017 | 27909932 |