| relationship between enhanced turnover of phosphatidylinositol and lymphocyte activation by mitogens. | 1. various lectins [phaseolus vulgaris phytohaemagglutinin, glycine max (soy-bean) agglutinin, triticum vulgaris (wheat-germ) agglutinin and axinella polyploides agglutinin] and antibodies to pig ig (immunoglobulin) that are found by pig lymphocytes were assessed in terms of their capacities to stimulate lymphocyte transformation and to enhance phosphatidylinositol turnover. transformation was measured after 45h of culture by incorporation of [6-(3)h]thymidine into dna, whereas phosphatidylinosi ... | 1975 | 167716 |
| [effect of various processes on the protein quality of food based on corn and soybeans]. | the present study deals with the effect of various processing methods on the protein values of maisoy, a blend of 30% whole soybean and 70% corn. the processes evaluated were: a) lime cooking using calcium hydroxide; b) toasting, and c) extrusion cooking, using an equipment known as the brady crop cooker. the same blend of soybean/corn was processed by the same three methods. in addition, a sample of maisoy--blend produced in bolivia with the extrusion equipment previously indicated--was also ev ... | 1977 | 563218 |
| autotrophic growth of h2-uptake-positive strains of rhizobium japonicum in an atmosphere supplied with hydrogen gas. | previous research from this laboratory has demonstrated co(2)-fixing and h(2)-uptake capacities of certain strains of rhizobium japonicum. in this report we have shown that sr, a h(2)-uptake-positive (hup(+)) strain of r. japonicum, is capable of autotrophic growth with h(2) as the energy source. growth occurred on mineral salts/vitamins/noble agar, mineral salts/vitamins liquid medium (0.27 mug of c as vitamins per ml), and in mineral salts liquid medium with no added vitamins when cultures wer ... | 1979 | 287019 |
| nitrogen fixing activity in rhizobium japonicum separated from plant cell cultures. | induced by soy bean tissue cultures in socalled "tissue chambers", rhizobium japonicum str. 61-a-96 developed nitrogenase activity separated from the plant cells. the activity proceded for 48 h with a rate of 1 x 10(-8) nmol c2h4h-1 cell-1, which is about 6% of the activity measured for bacteroids from rhizobium japonicum in nodules of glycine max. | 1975 | 130001 |
| inhibition of ige and compound 48/80-induced histamine release by lectins. | lectins from ricinus communis and glycine max, as well as wheat germ agglutinin and concanavalin a, caused a dose-dependent release of histamine from mast cells present in the mixed peritoneal cells from the rat. in addition, histamine release in an ige-mediated and a compound 48/80-mediated reaction was inhibited in cells which had been pretreated with these lectins. with concanavalin a and the r. communis lectin both effect were prevented by the addition of the appropriate monosaccharides to t ... | 1975 | 51003 |
| effect of lime-pelleting on nodulation and yield of soybean grown in acid soil. | an experiment for studying the effect of lime-pelleting of inoculated seed of soybean glycine max. (l.) merr. on nodulation, growth, and grain yield was undertaken in red sandy loam of bangalore, having a ph of 4.0. the results indicated that inoculation alone increased significantly nodulation, plant dry weight, and grain yield. but inoculation plus lime-pelleting significantly increased the dry weight of nodules and plants, but not the grain yield. however, lime-pelleting was found to be benef ... | 1977 | 24961 |
| alpha-d-galactosidase from soybeans destroying blood-group b antigens. purification by affinity chromatography and properties. | alpha-d-galactosidase was isolated from untoasted soybean meal and purified to homogeneity by affinity chromatography on n-epsilon-aminoacaproyl alpha-d-galactopyranosylamine-sepharose. the purified enzyme destroyed the b-specificity of human ovarian cyst b-glycoprotein with an accompanying increase in h-specificity, and converted human type-b erythrocytes to type o. the enzyme consists primarily of a tetramer, molecular weight 150 000 +/- 5 000 at ph 4.0 and of a monomer, molecular weight 40 00 ... | 1977 | 19253 |
| production of vitamin b-12 in tempeh, a fermented soybean food. | several varieties of soybeans contained generally less than 1 ng of vitamin b-12 per g. it was found that use of a lactic fermentation typical of tropical conditions during the initial soaking of the soybeans did not influence the vitamin b-12 content of the resulting tempeh. pure tempeh molds obtained from different sources did not produce vitamin b-12. it was found that the major source of vitamin b-12 in commercial tempeh purchased in toronto, canada, was a bacterium that accompanies the mold ... | 1977 | 563702 |
| genetic control of symbiotic nitrogen fixation in soybeans. | | 1977 | 562661 |
| chronic mycotoxicosis in chicks caused by toxins from phomopsis grown on soybeans. | | 1977 | 561808 |
| sensory factors which affect the acceptance of raw and heated defatted soybeans by rats. | the acceptability of heated soybean meal (hs) compared to raw soybean flakes (rs) was studied by means of diet selection in free choice situations. the selection by rats of diets containing hs over those containing rs was shown in short- and long-term preference tests. texture did not seem to be an important factor in the acceptability difference between hs and rs. rats selected a less nutritious diet containing rs + sodium saccharin (appealing taste) over a diet containing hs + sucrose octaacet ... | 1977 | 561170 |
| effect of rj1rj1 (non-nodulating) soybeans on nodulation of near isogenic rj1rj1 plants in nutrient culture. | an earlier proposal (can. j. microbiol. 7: 851; 1961) that rj1rj1 (non-nodulating) soybeans (glycine max (l.) merr.) excrete a substance that inhibits nodulation of rj1 rj1 (nodulating) plants was tested. using near isogenic lines (isolines) of "clark" and "harosoy" soybeans, we consistently found nonsignificant reduction in nodule number and acetylene reduction per rj1rj1 plant grown in association with their rj1rj1 counterparts: these results suggest that a nodulation inhibitor is not associat ... | 1977 | 560904 |
| ultrastructure of soybean nodules. i: release of rhizobia from the infection thread. | root nodules on soybeans (var. clark 63) were examined by transmission electron microscopy 10-12 days after seed inoculation and planting. the cell infection process appeared identical in both effective nodules, induced by rhizobium japonicum strain 138 (usda) and in ineffective nodules, induced by strain 8-0 (iowa). electron micrographs are presented which suggest that rhizobia are freed from the infection thread by disintegration of the thread wall and compartmentalization of the distintegrat ... | 1977 | 559533 |
| isolectins from soybean (glycine max). | the major lectin in seeds of a soybean cultivar (glycine max cv d68-127) has been purified to apparent homogeneity by hydroxyapatite and deae-cellulose chromatography. in the latter, the behavior of the lectin was similar to that of the minor isolectins previously described in other soybean cultivars. molecular weights of 92 000 for the molecule and 23 000 for the subunits were determined by gel filtration and sodium dodecyl sulfate-gel electrophoresis; these are smaller than those previously re ... | 1977 | 558805 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| bacteriocin-like substances produced by rhizobium japonicum and other slow-growing rhizobia. | bacteriocin-like substances were commonly produced by slow-growing rhizobium japonicum and cowpea rhizobia on an l-arabinose medium. antagonism between strains of r. japonicum was not detected in vitro; however, such strains were often sensitive to some bacteriocins produced by cowpea rhizobia. inhibitory zones (2 to 8 mm from colony margins), produced by 58 of 66 r. japonicum test strains, were reproducibly detected with corynebacterium nebraskense as an indicator. quantitative production was n ... | 1978 | 570016 |
| conversion of glycine max seed agglutinins from nonspecific to anti-(a + b). | the seeds of glycine max contain agglutinins which are typically nonspecific in their reactivity. our investigations show that the phytagglutinins in gm can be converted from nonspecific to anti-(a + b) after the lectin is absorbed with horse red cells. the anti-a and anti-b fractions can be further separated by suitably absorbing the lectin with human red cells. the lectin absorbed with horse red cells or with group-0 human red cells shows an a-stressed activity. | 1979 | 488297 |
| mycological survey of selected health foods. | a survey was conducted to compare the total viable fungal content and the number of different mold species encountered in 10 types of health foods labeled organically grown and in the same foods without such a label. the foods were wheat flour, corn meal, brown rice, figs, split peas, pinto beans, soybeans, walnuts, pecans, and peanuts. results showed no consistent difference in either the total viable fungal content or the number of different mold species encountered between the labeled and unl ... | 1979 | 453829 |
| biosynthesis of a prostaglandin by a plant enzyme. | lipoxygenase-2 from soybeans catalyzes the oxygenation of arachidonic acid to form significant amounts of a prostaglandin product. results obtained with the dithionite-reduced derivatized product upon combined gas chromatography-mass spectroscopy are consistent with structures of the stereoisomers of 9,11,15-trihydroxyprosta-5,13-dienoic acid. a portion of the material formed by reduction of the enzyme product with dithionite reacts positively in a radioimmunoassay against rabbit anti-prostaglan ... | 1978 | 412847 |
| [thermal inactivation and storage behavior of technologically important enzymes. ii. lipase from geotrichum candidum and lipoxygenase from soybeans]. | the thermal inactivation of lipase out of geotrichum candidum and of lipoxigenase out of soybeans was investigated in phosphate buffer solution. comparable to peroxidase, a sharp bend was observed in the inactivation curves of both enzymes. the z-value of 19 degrees c for lipase out of geotrichum candidum was higher than the values indicated in the pertinent literature for pancreas lipase and milk lipase. the z-value for soy lipoxigenase was found to be 9.8 degrees c. | 1977 | 408985 |
| proteinase inhibitors in severe inflammatory processes (septic shock and experimental endotoxaemia): biochemical, pathophysiological and therapeutic aspects. | plasma levels of antithrombin iii, alpha 2-macroglobulin and inter-alpha-trypsin inhibitor, as well as those of various clotting, complement and other plasma factors, were significantly decreased in 18 patients suffering from hyperdynamic septic shock. a similar statistically significant reduction of the concentrations of several plasma factors (prothrombin and antithrombin iii, plasminogen and alpha 2-plasmin inhibitor, complement factor c3 and clotting factor xiii) was observed in experimental ... | 1979 | 399895 |
| [soybeans and human nutrition]. | | 1979 | 390878 |
| somatic mosaicism in plants with special reference to somatic crossing over. | plant systems in use for the detection of environmental mutagens appear capable of detecting all types of genetic effects which can be studied in animals. the study of somatic mosaicism, however, is better developed in plants than in higher animals. a case is presented here which shows the ability of plant systems in analyzing a host of genetic end points, including chromosome aberrations like deletions, somatic crossing over, numerical inequality, gene conversion, paramutations and point mutati ... | 1978 | 367771 |
| potential for the use of germinated wheat and soybeans to enhance human nutrition. | wheat and soybeans are the major agricultural exports of the united states. the u.s. sells more of each crop than any other nation. soybeans are the main staple in china, but the u.s. sells more soybeans than china grows. for hundreds of millions of other people, wheat is the main staple. and yet, most americans eat whole grains of neither wheat nor soybeans. in the united states, many nutrients of wheat and soybeans are lost in processing or are fed to animals. a highly significant share of the ... | 1978 | 364942 |
| hypertrophy and hyperplasia in the endocrine and exocrine pancreas of rats fed soybean trypsin inhibitor or repeatedly injected with pancreozymin. | rats were 1) fed raw soybeans for 30 days, 2) given trypsin inhibitor as drinking water for 7 days, or 3) repeatedly injected with cholecystokinin-pancreozymin (cck-pz) for 7 days. after all these treatments the pancreas of the animals was markedly enlarged. microscopic examination revealed, besides hypertrophy of acinar cells, increased mitotic figures in the acinar, centro-acinar, intercalated portion and excretory duct cells. the islets of langerhans were found conspicuously enlarged due to m ... | 1976 | 779717 |
| circular dichroism studies on the effects of ethanol on the conformation of alpha1-acid glycoprotein, alpha1-antitrypsin, deoxyribonuclease, pepsinogen, soybean trypsin inhibitor and unfolded ribonucleases. | the effects of 25 to 75 volume-% ethanol on conformation of human serum alpha1-acid glycoprotein, human serum alpha1-antitrypsin, pancreatic deoxyribonuclease i, porcine pepsinogen, the "kunitz" trypsin inhibitor from soybeans, and oxidized as well as reduced and s-carboxymethylated ribonucleases were tested by the circular dichroism (cd) probe. it was found that 25 volume-% ethanol had a slight effect, whereas 50--75 vol.-% alcohol significantly altered the conformation. the tertiary structure ... | 1978 | 306838 |
| elastases from human and canine granulocytes, ii. interaction with protease inhibitors of animal, plant, and microbial origin. | inhibitors of animal, plant, and microbial origin were tested against human and canine granulocytic elastases. the trypsin-chymotrypsin inhibitors from dog submandibular glands, from soybeans (bowman-birk) and from chickpeas show strong interaction with these proteases (ki = 10(-8) - 10(-9)m). the trypsin-kallikrein inactivator of bovine organs (trasylol) is not active against granulocytic elastases or against human granulocytic cathepsin g. elastatinal, a specific inhibitor of elastases, isolat ... | 1977 | 300070 |
| comparative nutritive value, amino acid content, chemical composition, and digestibility in vitro of vegetable- and grain-type soybeans. | | 1978 | 564917 |
| adsorption and selection of rhizobia with ion-exchange papers. | ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ... | 1975 | 242293 |
| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| grouping of rhizobial strains--a method based on symbiotic characteristics. | twenty strains of rhizobium japonicum and non-inoculated control were used to study seven symbiotic characteristics with two varieties of soybean (glycine max). the strains were then grouped on the basis of these symbiotic characteristics, using mahalanobis' d2 statistical method. eight groups were formed in which two strains stood distinctly aloof, indicating thereby the exceptional nature of these strains over others in their symbiotic behaviour. this method is suggested for selecting exceptio ... | 1977 | 565116 |
| stepwise selection of efficient rhizobial cultures through cultural characteristics. | nodulation and shoot nitrogen of two varieties of soybean (glycine max) were studied with twenty strains of rhizobium japonicum. a number of cultural characteristics of the strains in isolation to the symbiotic system were also studied. a stepwise selection method was employed for detecting efficient cultures through the cultural characteristics which showed association with the steps in the symbiotic system. nodulation of one variety was found to be associated with the dehydrogenase activity an ... | 1977 | 565117 |
| changes in rat pancreatic protein synthesis after a single feeding with diets containing raw or heated soybeans. | the patterns of protein synthesis following the administration of a single meal containing defatted ground soybeans (rsd) or heated defatted ground soybeans (hsd) were compared. a double label method was used so that the determination of the relative rate of synthesis of an enzyme could not be obscured by a possible alteration of the activity or quantity of the enzyme. rats were given one meal of rsd or hsd and were subsequently killed at different times after the meal. eight hours after the mea ... | 1978 | 566785 |
| somatic crossing-over in glycine max (l.) merrill: activation of dimethyl nitrosoamine by plant seed and comparison with methyl nitrosourea in inducing somatic mosaicism. | the soybean system used for detecting environmental mutagens is analyzed for various types of spots on the leaves of heterozygous y11y11 plants and homozygous y11y11's induced by a nitrosoamine (dimethyl nitrosoamine, dmn) and a nitrosoamide (methyl nitrosourea, mnu). it is shown that the nitrosoamine can be "activated" by the seed (is converted to a true mutagen) without the addition of nadph or s-9 fraction of the liver homogenate as is necessary in animal tissue culture or bacterial studies. ... | 1978 | 569252 |
| improvement of the protein quality of corn with soybean protein. | in most central american countries, lime-treated corn provides 31% of the total protein and 45% of the energy intake, and beans 24% of the protein and 12% of the calories. such diet is low in protein quality and quantity, as well as in energy. to overcome these deficiencies, corn can be supplemented either with its limiting amino acids, lysine and tryptophan, or better still, with whole soybeans which improve not only the amount and quality of the protein consumed but, because of their high oil ... | 1978 | 569424 |
| soy protein utilization in food systems. | soy protein products are utilized in food systems as whole beans, flours and grits, concentrates and isolates, and textured products. soy proteins play a significant role in food systems as a source of supplementary and complementary protein and contribute functional properties such as solubility, water absorption, viscosity, emulsification, texture, and antioxidation. whole soybeans are processed into snack foods, beverages, and fermented foods. soy protein is an ideal supplement for cereal pro ... | 1978 | 569429 |
| evaluation of sclerotinia sclerotiorum as a potential mycotoxin producer on soybeans. | solvent extracts of sclerotinia sclerotiorum sclerotia were nontoxic to mice and chicken embryos; psoralens were not detected. solvent extracts of soybeans inoculated with 10 strains of s. sclerotiorum were toxic on injection but nontoxic on per os administration to mice. the presence of chlorinated hydrocarbons in the soybeans may partially help explain toxicity by intraperitineal injection. | 1978 | 569464 |
| gas-liquid chromatographic determination of sodium 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate residues on soybeans and foliage, soil, milk, and liver. | residues of sodium 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate and its free acid are determined by treating a sample extract with diazomethane to convert the residues to methyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate. this compound is purified by chromatography on florisil and measured by electron capture gas-liquid chromatography. the method has been used on soybeans, soybean foliage, soil, milk, and liver. it can detect 0.01 ppm with recoveries of 70--75%. | 1978 | 569657 |
| genetically marked rhizobium identifiable as inoculum strain in nodules of soybean plants grown in fields populated with rhizobium japonicum. | the fate of an inoculum strain of rhizobium japonicum was studied using a genetically marked strain i-11o subline carrying resistance markers for azide, rifampin, and streptomycin (i-110 ars). at the time of planting into a field populated with r. japonicum, seeds of soybean cultivars kent and peking were inoculated with varying cell densities of strain i-110 ars. at various times during the growing season, surface-sterilized root nodules were examined for the presence of the inoculum strain by ... | 1978 | 570015 |
| inhibition of human and rat pancreatic proteinases by crude and purified soybean proteinase inhibitors. | effects of proteinase inhibitors on total proteolytic activity and trypsin and chymotrypsin activity in human pancreatic juice were determined separately. purified inhibitors as well as crude extracts of raw soybeans completely inhibited trypsin and chymotrypsin activity while 40 to 50% of the total proteolytic activity remained. inhibition experiments with 1,10-o-phenanthroline showed that this residual proteolytic activity was due mainly to carboxypeptidase a and b. comparative studies with ra ... | 1979 | 571012 |
| the nutritive value of dehulled soybeans fermented with aspergillus oryzae or rhizopus oligosporus as evaluated by rats. | | 1979 | 571903 |
| production and nutritive value of soybeans. | soybean world production has been increasing at a rate of 5.2% per year (average yield is around 1,400 kg/ha). this production has been solely used for oil extraction and the protein meal obtained for animal rations, but lately it is being used for human consumption. brazil, the third largest producer, has had a yearly rate of production increase of 32% in the last years. average yields in brazil are still low (around 1,500 kg/ha), but in experimental results, yields over 3,000 kg/ha have been o ... | 1978 | 572663 |
| carbohydrate metabolism in chicks after administration of toxins from a phomopsis sp. | soybeans inoculated with phomopis sp. were incubated for weeks and then extracted with methanol and ether, and the culture filtrates were intubated into crops of chicks. activities of glucose-6-phosphatase and glucose-6-phosphate dehydrogenase in liver were increased, and glycogen stores and plasma glucose concentrations were reduced. histologically, severe hepatocytic necrosis was found in chicks treated with the toxic culture filtrate. significant clinical signs were loss of body weight and re ... | 1979 | 573612 |
| vesicular-arbuscular mycorrhiza and nodulation in soybean. | dual infections of glycine max with va endophytes and rhizobium, compared with rhizobium alone, increased the number and weight of nodules significantly in natural field soil and obviated the need of phosphate application for successful nodulation. | 1979 | 574481 |
| studies on soybean trypsin inhibitors. x. isolation and partial characterization of four soybean double-headed proteinase inhibitors. | four bowman-birk type double-headed inhibitors (b, c-ii, d-ii, and e-i) were isolated from soybeans. inhibitor b was different from bowman-birk inhibitor only in chromatographic behavior. one mole of c-ii inhibited one mole each of bovine trypsin and bovine alpha-chymotrypsin, probably at the same site, and porcine elastase at another reactive site. in the ordinary assay system d-ii and e-i inhibited only trypsin activity at a non-stoichiometric inhibitor-enzyme ratio of 1:1.4, and the complexes ... | 1977 | 599140 |
| localization of carbohydrate components in rat colon with fluoresceinated lectins. | cryostat sections of rat descending colon were studied by fluorescence microscopy after exposure to conjugates of fluorescein isothicoyanate with lectins from glycine max (soybean), triticum vulgaris (wheat germ), ricinus communis (castor bean), ulex europaeus, (gorse), dolichos biflorus (horse gram) and canavalia ensiformis (concanavalin a) (jack bean). no two lectins showed identical patterns of fluorescence. fitc-conjugates of soybean and d. biflorus lectins reacted strongly with the mucus pr ... | 1978 | 670681 |
| cummings memorial lecture - 1975. the market basket: food for thought. | the world food crisis is as critical today as when it was debated at the 1974 world food conference in rome. since the united states and canada-and to a lesser extent, australia and new zealand-lead in the production of corn, wheat and soybeans, the north american "bread basket" has become the "market basket" of the world. for welfare, economic, and political reasons, our energies, resources, and deliberations must be expanded toward optimum production of wholesome food products. i do not recomm ... | 1975 | 779439 |
| the meadow vole as an experimental animal. | a meadow vole colony has been in operation at the pennsylvania state university for 6 yr. the breeding colony consists of 16 breeding harems of 1 male and 2-4 females per cage. over 2,500 weanlings have been available each year for various experiments. primary emphasis has been directed toward determination of the weanling's nutritional requirements. adults have been used to study plant factors associated with the palatability of forages. this information has been used to devise specific bioassa ... | 1976 | 787673 |
| integrated pest management in the u.s.: progress and promise. | in the u.s., where heavy use of insecticides has been commonplace for years, the development of proper integrated insect pest control cannot get underway unless there is a changed use pattern for such chemicals. a changed use pattern, however, cannot be accomplished without much study to establish the requirements for integrated control for each major crop situation. in this paper recent developments in a number of crop areas in the u.s. in which the necessary study has been begun are reviewed. ... | 1976 | 789064 |
| studies of folic acid compounds in nature. iv. folic acid compounds in soybeans and cow milk. | folate compounds in soybean and cow's mild were identified by sephadex chromatography and differential microbiological assaymsoybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-cho-h4pteglun(1-6) constituted 65-70% of total folate activity; cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). in contrast to soybean, 90-95% of milk folate was in the 5- ... | 1975 | 804977 |
| the amino-acid sequence of leghemoglobin component a from phaseolus vulgaris (kidney bean). | 1. leghemoglobin component a from phaseolus vulgaris (kidney bean) was digested with trypsin; 15 tryptic peptides and free lysine were purified and the amino acid sequences of the peptides determined. 2. the internal order of the tryptic peptides was determined by the bridge peptides obtained from the thermolytic digest and the dilute acid hydrolyzate of kidney bean leghemoglobin a; 12 thermolytic peptides and two acid hydrolysis peptides were purified and the sequences were partially or complet ... | 1975 | 809270 |
| discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. | rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ... | 1976 | 818969 |
| improved method for preparing anaerobic bacteroid suspensions of rhizobium leguminosarum for the acetylene reduction assay. | a method using ethylenediaminetetraacetate (edta) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. the high level of acetylene reduction by these bacteroids is comparable to that of intact plants. reproducibility of the edta-toluene treatment is, on the average, within 5%. preliminary experiments with soybeans indicate that the edta-toluene method might be applicable to other legumes as well. | 1976 | 820257 |
| hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of rhizobium japonicum. | hydrogen evolved by nitrogenase may be recycled by a hydrogenase present in some legume nodules. anoka and portage cultivars of soybeans were inoculated with each of 8 and 24 strains, respectively, of rhizobium japonicum and surveyed for h2 evolution and c2h2 reduction rates nodule weight, and plant dry weight. six of the strains (3ilb 110, usda 122, usda 136, 3ilb 6, 3ilb 142, and 3ilb 143) which exhibited no h2 evolution in air were shown to take up h2. the relative efficiencies of nitrogenase ... | 1978 | 565672 |
| nutrient comparison of fresh and field-dried, green-seeded soybeans. | nutrient composition and biologic utilization of cooked, dried, and ground meals prepared from fresh and field-dried, green-seeded edible soybeans were evaluated. on a dry-weight basis, nutrient content of the fresh and field-dried meals were comparable for protein, fat, calcium, phosphorus, magnesium, copper, and iron; fresh beans tended to have higher zinc content than the field-dried beans. nutrient values for the green-seeded soybean meals were comparable to published values for full-fat soy ... | 1978 | 566776 |
| aflatoxin occurrence on raw and cooked york soybeans inoculated with three aspergillus isolates. | raw and cooked soybean media were inoculated in separate experiments with aspergillus flavus nrrl 3251, a. flavus atcc 15548, and a. parasiticus nrrl 2999 isolates. the toatl quantity of aflatoxins produced and the percentage distribution of aflatoxins b1, b2, g1, and g2 varied with the state of the medium (raw or cooked) and with the fungal isolate used. cooked soybean medium supported higher aflatoxin productions by a. flavus nrrl 3251 and a. parasiticus nrrl 2999 than did raw medium. larger q ... | 1976 | 944693 |
| fungus-fermented soybeans benefit the life cycle of japanese quail (coturnix coturnix japonica). | feeding quail chicks diets containing soybeans fermented with two cultures of aspergilli (a. oryzae n.r.r.l. 451 and a. oryzae n.r.r.l. 506) resulted in significantly superior weight gains (p less than 0.05) through a 4-week growth period and confirmed previous observations made with identical cultures in broiler studies. subsequent hen-day egg production and egg size were changed little by diets containing fermented soybeans. the numerical increases in fertility and hatchability were not signif ... | 1976 | 945574 |
| studies on seed coat toxicity to rhizobia of urid (phaseolus mungo), mung (phaseolus aureus), and soybean (glycine max). | | 1976 | 947116 |
| determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain. | reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. the ability of the method to distinguish between arginine and lysine residues was examined. trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (kunitz), peanuts and porcine seminal pla ... | 1976 | 964925 |
| relationship between food containing "natto" (fermented soybeans) and the blood pressure of shr. | | 1976 | 985891 |
| failure of soybean trypsin inhibitor to exert deleterious effects in calves. | to evaluate nutritional effects induced in calves by feeding soybean trypsin inhibitor, 16 calves were fed 1) raw soybeans, 2) heated soybeans, 3) heated soybeans plus soybean trypsin inhibitor, 4) heated (raw soybeans plus soybean trypsin inhibitor). ration 1 caused depression of growth and reduced digestibility of protein and fat as compared to ration 2. no differences were significant in calves fed rations 3 and 4. the weights and enzymatic activities of pancreas were similar in all groups. s ... | 1976 | 986409 |
| thin layer chromatographic determination of sterigmatocystin in cereal grains and soybeans. | a method has been developed for determining sterigmatocystin in yellow and white corn, barley, oats, rye, sorghum, brown and wild rice, and soybeans. a partition column packed with activated magnesium silicate was used for cleanup. averaged recoveries are 104% in white corn, 114% in rye, 100% in oats, 134% in brown rice, 96% in barley, 105% in sorghum and wild rice, and 92% in soybeans. the limit of sensitivity is 50 mug/kg for any of these commodities. | 1976 | 987035 |
| isolation and characterization of an ndvb locus from rhizobium fredii. | a gene (ndvb) in rhizobium meliloti that is essential for nodule development in medicago sativa (alfalfa), specifies synthesis of a large membrane protein. this protein appears to be an intermediate in beta-1,2-glucan synthesis by the microsymbiont. southern hybridization analysis showed strong homology between an ndvb (chvb) probe and genomic dna of r. fredii but not from bradyrhizobium japonicum. a cosmid clone containing the putative ndvb locus was isolated from a rhizobium fredii gene librar ... | 1992 | 1406255 |
| effects of treatment of whole fat soybeans or soy flour with formaldehyde to protect the polyunsaturated fatty acids from biohydrogenation in the rumen. | full-fat, ground soy flour (gsf) was treated with 37% formaldehyde (hcho) and evaluated by in vitro and in vivo criteria to determine the protection afforded linoleic acid against ruminal biohydrogenation when the materials described above were fed as a protein supplement to rations for growing lambs. the supplements compared were soybean meal (sbm), uked for 2 hours. organoleptic evaluations were conducted to determine if any flavor differences in meat from lambs fed these supplements could be ... | 1976 | 987164 |
| the amino acid sequences of two soybean double headed proteinase inhibitors and evolutionary consideration on the legume proteinase inhibitors. | two proteinase inhibitors, c-ii and d-ii, were isolated from soybeans. c-ii was shown to be an inhibitor of bovine trypsin [ec 3.4.21.4], bovine alpha-chymotrypsin [ec 3.4.21.1], and porcine elastase [ec 3.4.21.11], whereas d-ii inhibited only trypsin. the complete amino acid sequences of the two inhibitors establishors. on the basis of the specificities of the inhibitors and their homologies with other double-headed inhibitors, the reactive sites of c-ii seems to be alanine-22 for elastase and ... | 1976 | 988018 |
| rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. | four derivatives of rhizobium japonicum 110 were isolated on the basis of morphologically different colonies formed on yeast extract-mannitol-hm salts medium. all are able to nodulate lee soybeans. the bacteria-plant associations formed by each clone have measurable acetylene-reducing activity, but those formed by two of these clones (designated l1-110 and l2-110) are 5- to 10-fold less efficient than those formed by the others (designated i-110 and s-110). these derivatives were not detectable ... | 1976 | 988784 |
| immunochemical and chemical investigations of the structure of glycoprotein fragments obtained from epiglycanin, a glycoprotein at the surface of the ta3-ha cancer cell. | the structures of the carbohydrate chains present in fragments of a large-molecular-weight glycoprotein, epiglycanin, cleaved from the surface of viable ta3-ha murine mammary carcinoma ascites cells and purified by gel filtration, were studied by immunochemical and chemical methods. inhibitory activities for neuraminidase-treated and untreated glycoprotein material in the hemagglutination of nn-specific human erythrocytes by eight purified lectins were determined. excellent inhibition was obtain ... | 1975 | 1125949 |
| iron availability to rats from soybeans. | experiments were carried out to determine the effects of endogenous phytic acid in soybean seeds (glycine max, var. "amsoy") on the bioavailability of 59fe to iron-depleted rats. soybean plants were grown in nutrient solutions labeled with 59fe supplied at either 0.4 or 1.0 ppm iron in solution. immature and mature soybean seeds were harvested and fed to male rats as a single dose. the phytic acid content of the immature and mature seeds averaged 0.61 and 1.71% dry weight, respectively. varying ... | 1975 | 1167584 |
| the effect of the selective removal of hemagglutinins on the nutritive value of soybeans. | | 1975 | 1171131 |
| production of aflatoxin on soybeans. | probable factors influencing resistance to aflatoxin synthesis in soybeans have been investigated by using cultures of aspergillus parasiticus nrrl 3240. soybeans contain a small amount of zinc (0.01 mug/g) bound to phytic acid. autoclaving soybeans at 15 pounds (6803.88 g) for 15 min increases the aflatoxin production, probably by making zinc available. addition of zinc to both autoclaved and nonautoclaved soybeans promotes aflatoxin production. however, addition of varying levels of phytic aci ... | 1975 | 1171654 |
| bright greenish-yellow fluorescence and aflatoxin in agricultural commodities. | the corn milling industry has widely accepted the presence of bright greenish-yellow fluorescence under a black light as a presumptive indicator of aflatoxin (a poison produced by the mold aspergillus flavus). this test was applied to wheat, oats, barley, rice, coconut, white corn, yellow corn, peanuts, sorghum, and soybeans, and evaluated in the laboratory. our study supported the use of bright greenish-yellow fluorescence as a presumptive test for aflatoxin in wheat, oats, barley, corn, and so ... | 1975 | 1172410 |
| the distribution and binding of cadmium in oyster, soybean, and rat liver and kidney. | exposure to cd and/or 109cd has shown that the element was efficiently accumulated in oysters and soybeans as well as in rats. differential centrifugation of oyster, soybean, rat liver, and rat kidney homogenates followed by analysis showed that cd was associated primarily with proteins in the 105,000 x g supernatants. separation of these proteins by sephadex chromatography and subsequent analysis by atomic absorption spectroscopy or by radioactivity measurements revealed that cd in oysters and ... | 1975 | 1238062 |
| levels of urea in the blood and urine of rats fed raw and heated soybean meal. | protein from many sources show negative correlation between their biological values and the levels of urea in the blood or urine of rats or pigs. on the basis of the difference in protein quality between raw and heated soybeans, it would be predicted that there should be a higher level of urea in the blood and urine of rats fed raw soybean meal. in the present study, however, little or no difference in the levels of urea in the blood and urine of animals fed raw or autoclaved soybean meal could ... | 1975 | 1241608 |
| changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding. | lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) c57 bl/6j mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. the following lectins were used: canavalia ensiformis con a, triticum vulgaris (wga), glycine max (sba), griffonia simplicifolia (gs ii), arachis hypogaea (pna), pisum sativum (psa) and lens culinaris (lca). after incubation of frozen sections with con a, wga, gs ii, psa and lca a sarcoplasmic staining was obse ... | 1992 | 1290587 |
| modification of lectin binding in rat gut mucosa during experimental cholestasis. | glycoconjugate distribution on rat gut mucosa has been studied by using peroxidase-labelled lectins (lotus tetragonolobus, dolichos biflorus, arachis hypogaea and glycine max) after surgical interruption of the common bile duct. specimens from cholestatic rats were compared with sham-operated (simple laparotomy) and normal controls to determine which of the observed modifications could be due either to the operation itself or to the cholestasis. most of the modifications occurred in the duodenum ... | 1992 | 1295862 |
| isolation and characterization of rhizobitoxine mutants of bradyrhizobium japonicum. | to explore the role of rhizobitoxine in bradyrhizobium-legume symbiosis, 11 rhizobitoxine mutants of b. japonicum usda61 were isolated on the basis of their inability to synthesize the toxin in culture. each mutant is prototrophic and symbiotically effective on soybean, cowpea, siratro, and glycine soja. the rhizobitoxine mutants differ in their chlorosis phenotypes and rhizobitoxine production in planta. as expected, one group of mutant fail to make toxin in planta, resulting in the absence of ... | 1992 | 1317377 |
| effect of processing soybeans on their use by calves. | full-fat soybeans processed by grinding, roasting, or heating by microwave were used to supply the protein in rations fed holstein calves. nitrogen digestibilities of the rations were not significantly different. nitrogen retention for the roasted soybean ration was higher than that of raw or microwave-heated soybean rations. nitrogen solubility of the roasted soybeans was lower than that of the other soybeans. protein hydrolysis, as measured by a new in vitro technique, was higher for the roast ... | 1976 | 942983 |
| analysis of dna sequences transcribed at high levels in bradyrhizobium japonicum bacteroids but not necessary for symbiotic effectiveness. | five regions of the bradyrhizobium japonicum genome, which are transcribed at high levels in nitrogen-fixing soybean (glycine max) nodules, were identified. none of these regions contained previously identified genes (e.g., nif, nod, and fix genes) that are known to be essential for development of functional nitrogen-fixing nodules. to assess the role of these regions in the development of the b. japonicum-soybean symbiosis, we cloned and used them to construct b. japonicum strains, in which lar ... | 1992 | 1515666 |
| increasing polyunsaturation of milk fats by feeding formaldehyde protected sunflower-soybean supplement. | a practical means of protecting fats of a feed concentrate containing high polyunsaturated fatty acids is described. a ground mixture of 30% soybeans and 70% sunflower seeds was treated with 1% formaldehyde to protect the unsaturated lipids from microbial hydrogenation in the rumen. this was fed as a supplement to two holstein cows in amounts that were doubled weekly. these ranged from 524 to 8384 g/day and provided successively increasing intakes of 100, 200, 400, 800, and 1600 g of linoleic ac ... | 1976 | 944200 |
| replacement of forage or concentrate with combinations of soyhulls, sodium bicarbonate, or fat for lactating dairy cows. | a lactation study was performed from wk 4 to 19 of lactation to evaluate the ability of soyhulls with or without 1% sodium bicarbonate to replace corn silage and the ability of soyhulls, roasted soybeans, and rumen-inert fat to replace concentrate. all diets contained similar concentrations of ne(l) (tabular value), cp, and degradable protein. when forage ndf was reduced to 62.5% of total dietary ndf (32 to 36% ndf, depending on analytical method) with soyhulls, milk production and total tract n ... | 1992 | 1331214 |
| forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme. | glutamine synthetase (gs; ec 6.3.1.2) is present in different subcellular compartments in plants. it is located in the cytoplasm in root and root nodules while generally present in the chloroplasts in leaves. the expression of gs gene(s) is enhanced in root nodules and in soybean roots treated with ammonia. we have isolated four genes encoding subunits of cytosolic gs from soybean (glycine max l. cv. prize). promoter analysis of one of these genes (gs15) showed that it is expressed in a root-spe ... | 1992 | 1356501 |
| lectin staining studies on follicular atresia in house rat (rattus rattus). | topographic distribution of terminal and intercalary carbohydrate moieties were studied using horseradish peroxidase conjugated glycine max (gma), arachis hypogea (pna), lotus tetragonolobus (lta), bandirarea simplicifolia (bsa), and dolichos biflorus (dba) agglutinins. n-gal nac, alpha-d-gal, alpha-l-fuc, d-gal (beta-1-3)-d-gal nac, and alpha-beta-d gal nac linked glyco-conjugates were exposed during follicular atresia in oocyte, zona pellucida and granulosa cells. theca interna and interstitia ... | 1992 | 1365769 |
| proteins that rescue a-signal-defective mutants of myxococcus xanthus. | the asg mutants of myxococcus xanthus are defective in the production of an extracellular substance, called a-factor, that is required for expression of a set of fruiting body-specific genes. a-factor is released by wild-type cells (asg+) after 1 to 2 h of development. when a-factor is added to asg mutant cells, it restores expression of their a-factor-dependent genes. rescue of beta-galactosidase production in an asg mutant carrying the a-factor-dependent lacz transcriptional fusion (omega 4521 ... | 1992 | 1577696 |
| raw soybeans stimulate human pancreatic proteinase secretion. | intraduodenal instillation of raw soybeans stimulated pancreatic proteinase secretion in humans. raw soybeans almost abolished the activity of chymotrypsin and severely reduced (50%) the tryptic activity. immunoreactive tryptic and chymotryptic material simultaneously appeared in amounts 2 to 4 times basal concentrations. this increase, demonstrated with rocket immunoelectrophoresis, was begun within the first 10 min of soybean instillation. the enhanced secretion also persisted throughout the s ... | 1992 | 1377745 |
| cross-linking activity of the 14-kilodalton beta-galactoside-specific vertebrate lectin with asialofetuin: comparison with several galactose-specific plant lectins. | we have previously shown that plant lectins with a wide range of carbohydrate binding specificities can bind and cross-link (precipitate) specific multiantennary oligosaccharides and glycopeptides [cf. bhattacharyya, l., fant, j., lonn, h., & brewer, c. f. (1990) biochemistry 29, 7523-7530]. this leads to a new source of binding specificity: namely, the formation of homogeneous cross-linked lattices between lectins and carbohydrates. recently, we have demonstrated the existence of highly ordered ... | 1992 | 1382578 |
| a succinate transport mutant of bradyrhizobium japonicum forms ineffective nodules on soybeans. | biochemical evidence has shown that dicarboxylic acids actively support symbiotic nitrogen fixation by both fast- and slow-growing rhizobium. mutants defective in the active uptake of succinate have been previously described only in species of the fast-growing rhizobium. this article is a report on the isolation of mutants defective in dicarboxylate transport in a slow-growing species of rhizobium, bradyrhizobium japonicum. one of these presumptive dicarboxylate transport mutants, gts, was chara ... | 1992 | 1393826 |
| measurement of metabolizable energy in poultry feeds by an in vitro system. | a two-stage in vitro system (ivde) for estimating amen in poultry feeds was investigated. for 71 diets ranging from 2.2 to 3.4 kcal/g, the average amen was 2.889 kcal/g and the mean ivde value was 3.005 kcal/g. from the 71 diets, 30 (42.2%) showed differences between amen and ivde of less than .100 kcal/g and represented diets across the amen range of values. the statistical analysis of the data showed a standard error of the estimate (see) of .152 kcal/g for the 71 diets assayed. no clear diffe ... | 1992 | 1409234 |
| the consequences of the isotope effect on proline dehydrogenation rates estimated by the tritium loss method. | loss of tritium from a substrate is often used to estimate the rate of dehydrogenation. however, loss of 3h may be much slower than loss of h because of the tritium isotope effect. in order to assess the impact of the tritium isotope effect, loss of 3h from the c-5 position of proline during dehydrogenation by rat liver mitochondria and bacteroids from soybean (glycine max [l.] merrill) nodules was compared with appearance of 14c in products of [14c]proline dehydrogenation. incubations were carr ... | 1992 | 1416017 |
| characterization of lipid in loin and bacon from finishing pigs fed full-fat soybeans or tallow. | this experiment tested whether the lipid composition of pork could be altered by changes in dietary lipid, that what effects these changes would have on production and meat characteristics. pigs were fed diets containing 10 or 20%+ extruded full-fat soybeans (ffs) or 4% tallow for a 3- or 6-wk finishing period, with a corn-soybean meal diet fed as a control. seventy barrows and 70 gilts were allotted into seven treatment groups with four pens per group. analysis was on 28 experimental units, whi ... | 1992 | 1644691 |
| sequence of the fifth largest subunit of rna polymerase ii from plants. | an affinity-purified antibody raised against the fifth largest subunit of cauliflower (brassica oleracea) rna polymerase ii was used to screen an expression library and isolate an arabidopsis thaliana cdna clone. this cdna clone was used to isolate a soybean (glycine max) cdna clone, and both clones were sequenced. the open reading frames contain 176 amino acids and predict polypeptides of 19.5 and 19.6 kda for arabidopsis and soybean, respectively. the amino acid sequences of the arabidopsis an ... | 1992 | 1429663 |
| nolc, a rhizobium fredii gene involved in cultivar-specific nodulation of soybean, shares homology with a heat-shock gene. | rhizobium fredii strain usda257 does not nodulate soybean (glycine max (l.) merr.) cultivar mccall. mutant 257dh5, which contains a tn5 insert in the bacterial chromosome, forms nodules on this cultivar, but acetylene-reduction activity is absent. we have sequenced the region corresponding to the site of tn5 insertion in this mutant and find that it lies within a 1176bp open reading frame that we designate nolc. nolc encodes a protein of deduced molecular weight 43564. nucleotide sequences homol ... | 1991 | 1646377 |
| high osmotic pressure for pneumocystis carinii london resin white embedding enables fine immunocytochemistry studies: i. golgi complex and cell-wall synthesis. | a method for embedding pneumocystis carinii in hydrophilic resin (london resin white) has been developed for immunocytochemistry studies. using high osmotic pressure (about 850 mosmol) from fixation to embedding, this method improved the preservation of the fine structure as well as the antigenicity of rabbit- and scid mouse-derived p. carinii. cytochemistry studies were performed using colloidal gold-conjugated lectins (concanavalin a, glycine max, ulex europaeus) that reacted with the cytoplas ... | 1992 | 1438133 |
| characterization of cytochromes c550 and c555 from bradyrhizobium japonicum: cloning, mutagenesis, and sequencing of the c555 gene (cycc). | the major soluble c-type cytochromes in cultured cells of bradyrhizobium japonicum usda 110 comprised a co-reactive c555 (mr, approximately 15,500) and a non-co-reactive c550 (mr, approximately 12,500). levels of cytochrome per gram of soluble protein in aerobic, anaerobic, and symbiotic cells were 32, 21, and 30 nmol, respectively, for c555 and 31, 44, and 65 nmol, respectively, for c550. the midpoint redox potentials (em,7) of the purified cytochromes were +236 mv for c555 and +277 mv for c550 ... | 1991 | 1660457 |
| genetic relatedness of bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics. | forty-nine isolates of bradyrhizobium japonicum indigenous to a field where soybeans were grown for 45 years without inoculation were characterized by using four dna hybridization probes from b. japonicum. nifdk-specific hybridization clearly divided the isolates into two divergent groups. diversity in repeated-sequence (rs)-specific hybridization was observed; 44 isolates derived from 41 nodules were divided into 33 different rs fingerprint groups. cluster analysis showed that the rs fingerprin ... | 1992 | 1444394 |
| strains of bean common mosaic virus consist of at least two distinct potyviruses. | bean common mosaic virus (bcmv) consists of a large number of pathotypes and strains which have largely been identified by their characteristic interactions with a selected number of differential bean cultivars. the relationships among these strains and other potyviruses that infect legumes are complex, with indications that bcmv, blackeye cowpea mosaic virus (blcmv) and azuki bean mosaic virus (azmv) may be strains of the one virus. using high performance liquid chromatographic peptide profiles ... | 1992 | 1450767 |
| effect of heating on nutritional quality of conventional and kunitz trypsin inhibitor-free soybeans. | five 10-day chick growth experiments and an amino acid digestibility assay were conducted to assess the effect of steam heating on in vivo protein quality of raw, full-fat kunitz trypsin inhibitor-free soybeans (kfsb) compared with raw, conventional full-fat soybeans (csb). protein solubility in .2% koh was also evaluated as an in vitro test of in vivo protein quality for underprocessed csb. the csb and kfsb were autoclaved for 0, 3, 6, 9, 12, 15, 18, or 21 min at 121 c and 124 kpa. the soybeans ... | 1992 | 1454687 |
| length polymorphisms of simple sequence repeat dna in soybean. | the objective of this work was to ascertain the presence and degree of simple sequence repeat (ssr) dna length polymorphism in the soybean [glycine max (l.) merr.]. a search of genbank revealed no (ca)n or (gt)n ssrs with n greater than 8 in soybean. in contrast, 5 (at)n and 1 (att)n ssrs with n ranging from 14 to 27 were detected. polymerase chain reaction (pcr) primers to regions flanking the six ssr loci were used in pcr amplification of dna from 43 homozygous soybean genotypes. at three loci ... | 1992 | 1459432 |
| effects of feeding lactating dairy cows diets containing extruded soybeans and calcium salts of long-chain fatty acids. | four multiparous holstein cows averaging 36 dim and fitted with ruminal cannulas were utilized in a 4 x 4 latin square design to investigate the effects of feeding extruded whole soybeans and ca salts of long-chain fatty acids. treatments were diets containing 1) no added fat, 2) 16% extruded whole soybeans, 3) 16% extruded whole soybeans and 3% ca salts of long-chain fatty acids, and 4) 16% extruded whole soybeans and 6% ca salts of long-chain fatty acids. cows were fed for ad libitum intake a ... | 1992 | 1460132 |
| the transposable element tam1 from antirrhinum majus shows structural homology to the maize transposon en/spm and has no sequence specificity of insertion. | we present the genomic structure of tam1, a transposable element from antirrhinum majus. the tam1 element is 15.2 kb long and includes two genes that are transcribed to produce a 2.4 kb (tnp1) and a 5 kb mrna (tnp2). these transcripts partially overlap and the exons are scattered over the whole element. tnp1 encodes a 53 kda protein as deduced from the cdna sequence. the 5 kb transcript of tnp2 contains an open reading frame that shares 45% homology with part of the tnpd gene of en/spm from maiz ... | 1991 | 1715971 |
| allergenicity of peanut and soybean extracts altered by chemical or thermal denaturation in patients with atopic dermatitis and positive food challenges. | peanuts and soybeans are two of the six most common foods to cause food hypersensitivity reactions in children. we used the serum of 10 patients with atopic dermatitis and positive double-blind, placebo-controlled, food challenges to peanut and two patients with atopic dermatitis and positive double-blind, placebo-controlled, food challenges to soybean to investigate the change in ige-specific and igg-specific binding to these proteins altered by either chemical or thermal denaturation. we used ... | 1992 | 1460196 |