| amino acid sequence of chymotrypsin inhibitor eci from the seeds of erythrina variegata (linn.) var. orientalis. | the amino acids of the chymotrypsin inhibitor (eci) from the erythrina variegata seeds have been sequenced. the sequence was solved by analysis of peptides derived from the protein by enzymatic digestions with trypsin and staphylococcus aureus v8 proteinase, as well as by chemical cleavage with o-iodosobenzoic acid. the eci consists of 179 amino acid residues with a pyroglutamic acid as the n-terminal residue and has a calculated molecular weight of 19,791. comparison of this sequence with the s ... | 1993 | 7763417 |
| saving the ethnopharmacological heritage of samoa. | early european visitors to samoa tended to denigrate the authenticity and efficacy of samoan herbal medicine, yet bioassays indicate pharmacological activity in over 86% of samoan medicinal plants. novel anti-inflammatory compounds have been isolated from alphitonia zyzyphoides and erythrina variegata, and the anti-hiv compound prostratin has been isolated from homalanthus nutans. unfortunately, both samoan ethnopharmacology and samoan rain forests are threatened. in order to prevent logging, fu ... | 1993 | 8510467 |
| molecular cloning of cdna that encodes chymotrypsin inhibitor eci from erythrina variegata seeds and its expression in escherichia coli. | synthetic oligonucleotides representing all possible sequences of the n-terminal and internal amino acid sequences of the chymotrypsin inhibitor eci from erythrina variegata seeds were used to generate a probe specific for eci-related sequences by the polymerase chain reaction on the e. variegata genomic dna. a lambda phage cdna library constructed from poly(a+) rna from maturing seeds was screened with the eci gene thus obtained as a probe and characterized by dna sequencing. the cloned eci cdn ... | 1996 | 8987596 |
| antibacterial activity of isoflavonoids isolated from erythrina variegata against methicillin-resistant staphylococcus aureus. | to screen 16 isoflavonoids isolated from erythrina variegata (leguminosae) for their antibacterial activity against methicillin-resistant staphylococcus aureus (mrsa). | 2002 | 12460431 |
| antibacterial property of isoflavonoids isolated from erythrina variegata against cariogenic oral bacteria. | the antibacterial property of 7 compounds, isolated from erythrina variegata (leguminosae) by repeated silica gel column chromatography, against cariogenic oral bacteria was investigated. extensive spectroscopic study revealed that all were isoflavonoids. among them, 3,9-dihydroxy-2,10-di(gamma,gamma-dimethylallyl)-6a,11a-dehydropterocarpan (erycristagallin) showed the highest antibacterial activity against mutans streptococci, other oral streptococci, actinomyces and lactobacillus species with ... | 2003 | 12834009 |
| effect of supplementation of a basal diet of maize stover with erythrina variegata, gliricidia sepium or leucaena leucocephala on feed intake and digestibility by goats. | two 4 x 4 latin square design experiments were carried out. in experiment 1, four mature anglo-nubian x fiji local goats, pre-experimental body weight 25.0 +/- 0.6 kg, 22-24 months old, were used to study the effect of supplementation of a basal diet of maize stover with erythrina variegata (ev), gliricidia sepium (gs) and leucaena leucocephala (ll) on dry matter intake (dmi) and nutrient digestibility. maize stover treated with urea was used as a control diet. e. variegata was higher in crude p ... | 2004 | 14998316 |
| synergistic effects of mupirocin and an isoflavanone isolated from erythrina variegata on growth and recovery of methicillin-resistant staphylococcus aureus. | the phytochemical 2',4'-dihydroxy-8-gamma,gamma-dimethylallyl-2",2"-dimethylpyrano[5",6":6,7]isoflavanone (bidwillon b) was isolated from erythrina variegata and its antibacterial properties against methicillin-resistant staphylococcus aureus (mrsa) were investigated. bidwillon b inhibited the growth of 12 mrsa strains at minimum inhibitory concentrations (mics) of 3.13-6.25mg/l, while mics of mupirocin were 0.20-3.13 mg/l. the minimum bactericidal concentration (mbc) for bidwillon b and mupiroc ... | 2004 | 15325427 |
| six new constituents from the roots of erythrina variegata. | three new isoflavonoids, eryvarins m-o (1-3), two new 2-arylbenzofurans, eryvarins p and q (4 and 5), and a new 3-aryl-2,3-dihydrobenzofuran, eryvarin r (6), together with three known compounds, were isolated from the roots of erythrina variegata. the structures of these compounds were established by spectroscopic analysis. eryvarin r (6) is an unusual 3-aryl-2,3-dihydrobenzofuran derivative with a formyl (cho) group. eryvarin q (5) showed potent antibacterial activity against methicillin-resist ... | 2004 | 17191901 |
| effect of erythrina variegata seed extract on hyperlipidemia elicited by high-fat diet in wistar rats. | to investigate the effect of the methanolic extract of erythrina variegata (linn.) var orientalis (fabaceae) seeds (meev) in reducing the cholesterol levels and as well as antioxidant in experimentally induced hyperlipidemic rats. | 2010 | 21180471 |
| a new biisoflavonoid from the roots of erythrina variegata. | a new biisoflavonoid, biseryvarin a (1a), together with two known compounds were isolated from the roots of erythrina variegata. the structure of biseryvarin a was established on the basis of spectroscopic evidence. biseryvarin a is the first dimeric isoflavonoid possessing isoprenoid groups from the genus erythrina. biseryvarin a showed low activity against methicillin-resistant staphylococcus aureus (mrsa). | 2010 | 21213979 |
| three new constituents from the roots of erythrina variegata and their antibacterial activity against methicillin-resistant staphylococcus aureus. | two new isoflavonoids, eryvarins v and w (1 and 2, resp.), and a new chromen-4-one derivative, eryvarin x (3), along with three known isoflavonoids, 4-6, were isolated from the roots of erythrina variegata. their structures were established by spectroscopic analyses. compound 1 is a rare naturally occurring isoflavanone which possesses a oh group at c(3). among the new compounds 1-3, 2 exhibited a potent antibacterial activity against methicillin-resistant staphylococcus aureus (mrsa) strains. | 2011 | 21404431 |
| Effect of Erythrina variegata on experimental atherosclerosis in guinea pigs. | | 2011 | 22025860 |
| in-vitro anthelmintic activity of three bangladeshi plants against paramphistomum cervi and haemonchus contortus. | conventional plant-based therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out. | 2015 | 25868001 |
| two new isoflavanones from the roots of erythrina variegata. | two new isoflavanones, eryvarins y (1) and z (2), together with three known compounds (3-5) were isolated from the roots of erythrina variegata. the structures of 1 and.2 were established on the basis of spectroscopic data. the new compounds are peculiar isoflavanones, possessing both a 3-methylbut-2-en- 1-yl group and a 2-hydroxy-3-methylbut-3-en-1-yl group. among the isolates, orientanol e (5) exhibited potent antibacterial activity against methicillin- resistant staphylococcus aureus (mrsa) s ... | 2015 | 25924538 |
| effects of indian coral tree, erythrina indica lectin on eggs and larval development of melon fruit fly, bactrocera cucurbitae. | present study was undertaken to investigate the influence of d-galactose binding lectin from erythrina indica lam. on the eggs and second instar larvae (64-72 hr) of melon fruit fly, bactrocera cucurbitae (coquillett). the lectin from e. indica seeds was extracted and purified by affinity chromatography using asilofetuin linked porous amino activated silica beads. the effects of various concentrations (0, 125, 250, 500 and 1000 microg ml(-1)) of lectin were studied on freshly laid eggs (0-8 hr) ... | 2009 | 20120488 |
| [anti-tumor activity of erythrina variegata l. extract and its mechanism of action]. | the anti-tumor activities and mechanism of erythrina variegata l. extract were investigated. firstly, the mtt method was used to evaluate the inhibitory activity of the erythrina variegata l. extract on proliferation of cancer cell lines. moreover, in order to determine its anti-tumor effect in vivo, the lewis lung cancer mice model was established. by comparing the relative tumor proliferation rates, growth curves, inhibition rates of different groups, the anti-tumor effect was evaluated. furth ... | 2009 | 21351469 |
| hypoglycemic activity of erythrina variegata leaf in streptozotocin-induced diabetic rats. | erythrina variegata linn. (fabaceae), commonly known as tiger's claw, is a thorny deciduous tree grown in tropical and subtropical regions of eastern africa, southern asia, and northern australia. in india, its leaves are traditionally used for diabetes mellitus. | 2011 | 21281246 |
| erythrina variegata extract exerts osteoprotective effects by suppression of the process of bone resorption. | our previous study showed that erythrina variegata l. (ev) inhibited bone loss and improved bone properties in ovariectomised rats. the purpose of the present study is to investigate the potential mechanism involved in mediating the osteoprotective actions of ev. female sprague-dawley rats were fed a phyto-oestrogen-free diet and subjected to either ovariectomy or a sham operation. ovariectomised rats were treated with genistein (40 mg/kg) as well as low (200 mg/kg), medium (500 mg/kg) or high ( ... | 2010 | 20487580 |
| erythrina variegata linn: a review on morphology, phytochemistry, and pharmacological aspects. | this review gives an account of the current knowledge on the morphology, phytochemistry, and pharmacological aspects of erythrina variegata. e. variegata also called erythrina indica is a thorny deciduous tree growing to 60 feet tall. a wide range of chemical compounds have been isolated, mainly alkaloids, flavonoids, triterpenoids, and lectin. different parts of the plant have been used in traditional medicine as nervine sedative, collyrium in opthalmia, antiasthmatic, antiepileptic, antiseptic ... | 2010 | 22228954 |
| regulation and symbiotic significance of nodulation outer proteins secretion in sinorhizobium fredii hh103. | in this work we show that the sinorhizobium fredii hh103 ttsi gene is essential for the expression of the tts genes and secretion of nodulation outer proteins (nops). moreover, we demonstrate for the first time, to our knowledge, that the nod box preceding ttsi is necessary for nops secretion. ttsi is responsible for the transcriptional activation of nopx, nopa, rhcj and rhcq. we confirm that the s. fredii hh103 ttsi gene is activated by nodd1 and repressed by nolr. in contrast, nodd2 is not inv ... | 2008 | 18524937 |
| [effect of erythrina variegata on ca2+ homeostasis in ovariectomized rats and action mechanism]. | to study the effect of erythrina variegata (ev) on ca2+ homeostasis in ovariectomized rats and the regulation on gene expression in duodenum and kidney. | 2007 | 17583208 |
| purification and characterization of a trypsin inhibitor from seeds of murraya koenigii. | a protein with trypsin inhibitory activity was purified to homogeneity from the seeds of murraya koenigii (curry leaf tree) by ion exchange chromatography and gel filtration chromatography on hplc. the molecular mass of the protein was determined to be 27 kda by sds-page analysis under reducing conditions. the solubility studies at different ph conditions showed that it is completely soluble at and above ph 7.5 and slowly precipitates below this ph at a protein concentration of 1 mg/ml. the puri ... | 2007 | 17373557 |
| anti-osteoporotic effect of erythrina variegata l. in ovariectomized rats. | the present study was designed to investigate whether erythrina variegata l. (ev), which belongs to the leguminous family, exerted any beneficial effects on bone in ovariectomized rats. daily oral administration of the ev extract at 300 and 600 mg/kg for 14 weeks to rats prevented the ovx-induced increase in the serum ocn, alp, and urinary dpd levels. histomorphometric analysis of the proximal end of the tibia showed that the ev extract prevented the estrogen deficiency-induced decrease in trabe ... | 2007 | 16920298 |
| four new isoflavonoids from the stem bark of erythrina variegata. | bioassay-directed fractionation of the stem bark extract of erythrina variegata l. has resulted in the isolation of three new isoflavones: 5,4'-dihydroxy-8-(3,3-dimethylallyl)-2''-methoxyisopropylfurano[4,5:6,7]isoflavone (1), 5,7,4'-trihydroxy-6-(3,3-dimethylallyloxiranylmethyl)isoflavone (2), 5,4'-dihydroxy-8-(3,3-dimethylallyl)-2''-hydroxymethyl-2''-methylpyrano[5,6:6,7]isoflavone (3) and a new isoflavanone, 5,4'-dihydroxy-2'-methoxy-8-(3,3-dimethylallyl)-2'',2''-dimethylpyrano[5,6:6,7]isofla ... | 2006 | 16595969 |
| contribution of conserved asn residues to the inhibitory activities of kunitz-type protease inhibitors from plants. | plant kunitz-type protease inhibitors contain a conserved asn residue in the n-terminal region. to investigate the role of asn residue in protease inhibitory activities, erythrina variegata trypsin inhibitor a (etia), e. variegata chymotrypsin inhibitor (eci), and their mutants, etia-n12a and eci-n13a, were used. both mutants exhibit weaker inhibitory activities toward their cognate proteases than the wild-type proteins and were readily cleaved at reactive sites. furthermore, kinetic analysis of ... | 2005 | 15665491 |
| cytotoxicity induced by erythrina variegata serine proteinase inhibitors in tumor hematopoietic stem cell lines. | based on the soluble mtt tetrazolium/formazan assay, we evaluated the cytotoxicity of erythrina variegata proteinase inhibitors in some tumor hematopoietic stem cell lines. among the proteinase inhibitors, ebi, which belongs to the bowman-birk family of inhibitors, was cytotoxic in relatively differentiated cells such as molt4 and jurkat derived from acute t lymphoblastic leukemia (t-all) cells specifically, but etia and eci, which are classified into kunitz family inhibitors, did not. it was su ... | 1998 | 9692201 |
| indonesian medicinal plants. xxi. inhibitors of na+/h+ exchanger from the bark of erythrina variegata and the roots of maclura cochinchinensis. | through bioassay-guided separation of the methanol extracts of indonesian medicinal plants, three inhibitors of the na+/h+ exchange system, erythrinin b (2), euchrenone b10 (3), and 1,3,5-trihydroxy-4-(3-methylbut-2-enyl)xanthen-9-one (4), were isolated from the bark of erythrina variegata (fabaceae) (for 2) and the roots of maclura cochinchinensis (moraceae) (for 2, 3, 4). compounds 2, 3, and 4 significantly inhibited the na+/h+ exchange system of arterial smooth muscle cells, with minimum inhi ... | 1997 | 9353889 |
| cloning, expression, and mutagenesis of trypsin inhibitor etib from erythrina variegata seeds. | erythrina variegata trypsin inhibitors designated etia and etib belong to the kunitz family trypsin inhibitor, but etia is unique in its ability to inhibit tissue-type plasminogen activator, while etib is not. the cdna clone encoding etib was isolated from the seed cdna library constructed in the lambda phage lambda gt11. the etib cdna insert consists of 765 bp, including an open reading frame of 606 pb from atg to tga codons. the deduced amino acid sequence consists of 202 amino acids, having t ... | 1997 | 9214769 |
| the tissue-type plasminogen activator inhibitor etia from erythrina variegata: structural basis for the inhibitory activity by cloning, expression, and mutagenesis of the cdna encoding etia. | erythrina variegata trypsin inhibitor etia belongs to the kunitz inhibitor family, but is unique in its ability to bind and inhibit tissue-type plasminogen activator (tpa). a cdna clone encoding etia was isolated from the lambda gt11 cdna library using specific antiserum as a probe and characterized by nucleotide sequencing. the cloned etia cdna consists of 762 nucleotides and includes an open reading frame encoding a polypeptide of 198 amino acids. comparison of the deduced protein sequence and ... | 1997 | 9133614 |
| isolation and primary structure of proteinase inhibitors from erythrina variegata (linn.) var. orientalis seeds. | the kunitz-type trypsin inhibitors, etia and etib, and chymotrypsin inhibitor eci were isolated from the seeds of erythrina variegata. the proteins were extracted from a defatted meal of seeds with 10 mm phosphate buffer, ph 7.2, containing 0.15 m nacl, and purified by deae-cellulose and q-sepharose column chromatographies. the stoichiometry of trypsin inhibitors with trypsin was estimated to be 1:1, while that of chymotrypsin inhibitor with chymotrypsin was 1:2, judging from the titration patte ... | 1992 | 1369077 |
| isolation and characterization of isolectins from erythrina variegata seeds. | three isolectins were isolated from seeds of erythrina variegata (linn.) var. orientalis by ion-exchange chromatography, followed by affinity chromatography on lactose-sepharose 4b and acid-treated sepharose 4b columns. the purified isolectins (evli, evlii and evliii) are all specific for galactopyranosides and n-acetylgalactosamine, and their affinities for simple sugars are evliii greater than evlii greater than evli. evli and evliii are homodimers made up of an a-subunit of molecular mass 36, ... | 1992 | 1517319 |
| inhibitory potency of erythrina variegata proteinase inhibitors toward serine proteinases in the blood coagulation and fibrinolytic systems. | the erythrina variegata kunitz family trypsin inhibitors, etia and etib, prolonged the activated partial thromboplastin time (aptt) and also the prothrombin time (pt) of human plasma, but the kunitz family chymotrypsin inhibitor, eci, and bowman-birk family inhibitor, ebi, from e. variegata hardly prolonged these times. trypsin inhibitors etia and etib inhibited the amidolytic activity of factor xa, and etib but not etia inhibited plasma kallikrein. neither etia nor etib exhibited any inhibitory ... | 1996 | 8987561 |
| chemical structures of two subunits, a-subunit and b-subunit, of galactose-specific isolectins from erythrina variegata seeds. | we previously isolated galactose-specific isolectins, evli, evlii, and evliii, from the erythrina variegata seeds [j. chromatogr. 597, 207-211 (1992)]. the amino acid sequences of the two subunits, a- and b-subunits, of which the isolectins are composed, were determined. a comparison of the amino acid sequences of tryptic peptides from the two subunits revealed seven amino acid substitutions. among them, asn46, to which the oligosaccharide chain is linked in the a-subunit, is replaced by asp46 i ... | 1993 | 8276768 |
| identification, isolation and some properties of lectin from the seeds of indian coral tree [erythrina variegata (linn.) var. orientalis (linn.) merrill]. | a d-galactose-binding lectin agglutinating human erythrocytes has been purified from the seeds of the indian coral tree (erythrina variegata (linn.) var. orientalis (linn.) merrill] by affinity chromatography on acid-treated sepharose-6b gel. it has a higher reactivity for o-group erythrocytes. the lectin is a glycoprotein having a leucoagglutinating property. | 1981 | 7325983 |
| erythrina--chemical and pharmacological evaluation ii: alkaloids of erythrina variegata l. | | 1972 | 5050377 |
| structural requirements for the binding of oligosaccharides to immobilized lectin of erythrina variegata (linn) var. orientalis. | the structural requirements for the interaction of asparagine-linked oligosaccharide moieties of glycoproteins with erythrina variegata agglutinin (eva) were investigated by means of affinity chromatography on an eva-sepharose column. some of the branched poly-n-acetyllactosamine-type oligosaccharides obtained from human erythrocyte band 3 glycoprotein were found to show high affinity to eva-sepharose, whereas complex-type oligosaccharides were shown to have low affinity. hybrid type, oligomanno ... | 1990 | 2136348 |
| antiangiogenetic effects of anthranoids from alternaria sp., an endophytic fungus in a thai medicinal plant erythrina variegata. | endophytic fungi are known as a prolific source for the discovery of structurally interesting and biologically active secondary metabolites, some of which are promising candidates for drug development. in the present study, three anthranoids were isolated from an alternaria sp. endophytic fungus and evaluated for their antiangiogenic activity in a rat aortic sprouting assay, an ex vivo model of angiogenesis. of these three compounds, altersolanol (2) was further characterized and found to show a ... | 2013 | 23639188 |
| one-step separation of antioxidant compounds from erythrina variegata by high speed counter-current chromatography. | high speed counter-current chromatography (hsccc) was used for separation and purification of antioxidant compounds from ethyl acetate fraction of the stem bark of erythrina variegata. the optimal two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:4:1:4, v/v/v/v). after one-step hsccc separation, 75 mg of protocatechuic acid ( 1: ), 32 mg of chlorogenic acid ( 2: ) and 44 mg of caffeic acid ( 3: ) were purified from 420 mg of the ethyl acetate fraction. the purity ... | 2015 | 25209680 |
| dimeric erythrina alkaloids from the flower of erythrina variegata. | unprecedented dimeric erythrina alkaloids, spirocyclic (6/5/6/6) erythrivarine a (1) and spiro-fused (6/5/7/6) rings erythrivarine b (2), were isolated from the cultivated plant, e. variegata. the structures were determined on the basis of 1d and 2d nmr, ftir, uv, and mass spectroscopic data and x-ray crystal diffraction. the biogenetic relationship of 1 and 2 is proposed. | 2014 | 25470209 |
| molecular docking and analgesic studies of erythrina variegata׳s derived phytochemicals with cox enzymes. | secondary metabolites from plants are a good source for the nsaid drug development. we studied the analgesic activity of ethanolic extract of erythrina variegata l. (fabaceae) followed by molecular docking analysis. the analgesic activity of erythrina variegata l. is evaluated by various methods viz., acetic acid-induced writhing test, hot plate and tail immersion test. subsequently, molecular docking analysis has been performed to identify compounds having activity against cox-1 and cox-2 enzym ... | 2014 | 25489172 |
| phytochemical profile of erythrina variegata by using high-performance liquid chromatography and gas chromatography-mass spectroscopy analyses. | natural products derived from plant sources have been utilized to treat patients with numerous diseases. the phytochemical constituents present in ethanolic leaf extract of erythrina variegata (elev) were identified by using high-performance liquid chromatography (hplc) and gas chromatography-mass spectroscopy (gc-ms) analyses. shade dried leaves were powdered and extracted with ethanol for analyses through hplc to identify selected flavonoids and through gc-ms to identify other molecules. the h ... | 2016 | 27555226 |
| botanicals to control soft rot bacteria of potato. | extracts from eleven different plant species such as jute (corchorus capsularis l.), cheerota (swertia chiraita ham.), chatim (alstonia scholaris l.), mander (erythrina variegata), bael (aegle marmelos l.), marigold (tagetes erecta), onion (allium cepa), garlic (allium sativum l.), neem (azadiracta indica), lime (citrus aurantifolia), and turmeric (curcuma longa l.) were tested for antibacterial activity against potato soft rot bacteria, e. carotovora subsp. carotovora (ecc) p-138, under in vitr ... | 2012 | 22701096 |
| xanthoxyletin, a coumarin induces s phase arrest and apoptosis in human gastric adenocarcinoma sgc-7901 cells. | this study was conducted to explore the novel anticancer compounds from chinese herbs. during the process of screening, to evaluate the potential chemopreventive effect of natural compounds, xanthoxyletin was isolated from erythrina variegata. it has been reported that xanthoxyletin possesses antibacterial, fungicidal, and algicidal properties. in this study, we examined the antiproliferative effects of xanthoxyletin against sgc-7901 cells and its ability to induce apoptosis and cell cycle arres ... | 2011 | 21875271 |
| antifeedants from chinese medicinal herb, erythrina variegata var. orientalis, against maize weevil sitophilus zeamais. | the screening for insecticidal principles from several chinese medicinal herbs showed that the stem bark of erythrina variegata var. orientalis possessed significant feeding deterrence against maize weevils, sitophilus zeamais. bioassay-directed fractionation of the stem bark extract of e. variegata var. orientalis resulted in the isolation of two alkaloids, identified as erysopine and erysovine from their spectroscopic data. erysopine and erysovine possessed antifeedant activity against s. zeam ... | 2012 | 22474945 |
| effect of feeding different levels of foliage from erythrina variegata on the performance of growing goats. | the effect of feeding different levels of foliage from erythrina variegata on the performance of growing goats was studied using a local breed (ma t'ou) with an average initial body weight of 11.2 kg (sd = 0.9). twenty-four animals were allocated to a randomized design, with six animals (three males and three females) per treatment. the treatments were four different levels of replacement of the diet crude protein (cp) with cp from erythrina foliage (ef) at 0 % (e-0), 20 % (e-20), 40 % (e-40), a ... | 2012 | 22461199 |
| osteogenic activities of genistein derivatives were influenced by the presence of prenyl group at ring a. | our recent report indicated that the crude extract from stem bark of erythrina variegata l. (leguminosae) (ev) exerted beneficial effects against osteoporosis induced by estrogen deficiency in vivo. follow-up phytochemical study has isolated genistein-derivatives mainly in the form of prenylgenistein from this extract, including 6-prenylgenistein, 8-prenylgenistein, and 6, 8-diprenylgenistein. the present study was performed to investigate the structure-function relationship of these compounds o ... | 2008 | 19099220 |
| phytoremediation of petroleum hydrocarbons in tropical coastal soils. i. selection of promising woody plants. | this glasshouse study is aimed at evaluating tropical plants for phytoremediation of petroleum hydrocarbon-contaminated saline sandy subsurface soils. tropical plants were selected for their ability to tolerate high salinity and remove no. 2 diesel fuel in coastal topsoil prior to further investigation of the phytoremediation feasibility in deep contaminated soils. the residual petroleum-hydrocarbon contaminant at the john rogers tank farm site, a former petroleum storage facility, at hickam air ... | 2004 | 15341316 |
| eryvarins f and g, two 3-phenoxychromones from the roots of erythrina variegata. | two 3-phenoxychromones, eryvarins f and g, were isolated from the roots of erythrina variegata. their structures were established to be 3-(2,4-dihydroxyphenoxy)-7-hydroxy-6,8-di(3,3-dimethylallyl)chromen-4-one and 3-(2,4-dihydroxyphenoxy)-8-(3,3-dimethylallyl)-2,2-dimethylpyrano[5,6:6,7]chromen-4-one on the basis of spectroscopic and chemical evidence. eryvarins f and g are unusual 3-phenoxychromone derivatives with two isoprenoid groups. | 2003 | 12648545 |
| two diphenylpropan-1,2-diol syringates from the roots of erythrina variegata. | two new diphenylpropan-1,2-diols, eryvarinols a (1) and b (2), were isolated from the roots of erythrina variegata. their structures were elucidated as 1-(4-hydroxy-2-methoxyphenyl)-2-(4-hydroxy-3,5-dimethoxybenzoyloxy)-3-(4-hydroxyphenyl)propan-1-ol (1) and its 3"-prenyl derivative (2) on the basis of spectroscopic and chemical evidence. both these compounds are unusual diphenylpropan-1,2-diols with a syringyl group. | 2002 | 12502344 |
| chymotrypsin inhibitor from erythrina variegata seeds: involvement of amino acid residues within the primary binding loop in potent inhibitory activity toward chymotrypsin. | the stoichiometry of the interaction between erythrina variegata chymotrypsin inhibitor eci and chymotrypsin was reinvestigated by analysis of their complex with ultracentrifugation and with amino acid analysis of the components separated. the amino acid analysis clearly showed that the stoichiometry of eci and chymotrypsin was 1:1, though the apparent molecular mass of the complex was estimated to be 60 kda. to examine the contribution of leu64 (the p1 residue) to the inhibitory activity of eci ... | 1998 | 9722681 |
| amino acid sequence, glycan structure, and proteolytic processing of the lectin of vatairea macrocarpa seeds. | vml is a galactose-binding lectin isolated from vatairea macrocarpa seeds. by sds-polyacrylamide gel electrophoresis, vml is a glycoprotein composed of a major 32-34 kda double band (alpha-chain) and minor 22 kda and 13 kda bands. n-terminal sequencing of electroblotted samples showed that the 22 and 13 kda bands corresponded to c-(beta) and n-(gamma) terminal fragments of the alpha-chain, respectively. the primary structure of vml displays similarity with other leguminous lectins, particularly ... | 1998 | 9559667 |
| two new isoflavonoids from erythrina variegata. | two new isoflavonoids, eryvarin a (1) and eryvarin b (2) were isolated from the wood of erythrina variegata and their structures were elucidated on the basis of spectroscopic evidence. | 2000 | 10985092 |
| conformation of the primary binding loop folded through an intramolecular interaction contributes to the strong chymotrypsin inhibitory activity of the chymotrypsin inhibitor from erythrina variegata seeds. | we previously demonstrated that amino acid residues gln62 (p3), phe63 (p2), leu64 (p1), and phe67 (p3') in the primary binding loop of erythrina variegata chymotrypsin inhibitor (eci), a member of the kunitz inhibitor family, are involved in its strong inhibitory activity toward chymotrypsin [iwanaga et al. (1998) j. biochem. 124, 663-669]. to determine whether or not these four amino acid residues predominantly contribute to the strong inhibitory activity of eci, they were simultaneously replac ... | 1999 | 10393334 |
| analysis of the complex formed by erythrina variegata chymotrypsin inhibitor with chymotrypsin and properties of the peptides prepared from the inhibitor by a limited proteolysis. | the stoichiometry of erythrina variegata chymotrypsin inhibitor (eci) and chymotrypsin interaction was previously estimated to be 1:2 by a titration of inhibitory activity. in the present study, gel-permeation chromatography and reverse-phase hplc (rp-hplc) were employed to analyze the complex formed by the inhibitor and enzyme. the results showed that eci and chymotrypsin molecules undergo aggregation in the complex-forming buffer simultaneously with a binary complex consisting of one eci and o ... | 1997 | 9395338 |
| on a bowman-birk family proteinase inhibitor from erythrina variegata seeds. | a bowman-birk family proteinase inhibitor (ebi) was isolated from the seeds of erythrina variegata. the protein was purified by ion-exchange column chromatography on deae-cellulose followed by gel filtration on sephadex g-75. the stoichiometry with trypsin was estimated to be 1:1, while that with chymotrypsin was not obvious, as determined from the titration patterns of its inhibitory activities. the complete amino acid sequence of ebi was determined by sequencing tryptic and chymotryptic peptid ... | 1994 | 8056744 |
| studies on the causes of forest decline in nanshan, chongqing. | nanshan is located in the south suburb of chongqing, sichuan province in china. it is about 10 km from the city of chongqing. there are about 2000 ha of forest, mainly masson pines (pinus massoniana lamb) which have exhibited decline since the beginning of the 1980s. surveys of stands estimated that 85% of masson pines are injured. symptomology of masson pines includes tip necrosis of needles, thin crown, reduced needle length, premature abscission, branch dieback, reduced radial growth. the fol ... | 1990 | 24243326 |
| alkaloidal constituents of erythrina variegata bark. | | 1988 | 3212083 |
| investigation of erythrina spp. vii. chemical constituents of erythrina variegata var. orientalis bark. | the petroleum ether extractive of the bark of erythrina variegata var. orientalis was fractionated and shown to be composed of wax alcohols and wax acids, alkyl ferulates, alkyl phenolates, stigmasterol, sitosterol, campesterol and possibly citrostadienol/24-methylenelophenol. the ethanol extractive yielded chloroform-soluble and water-soluble bases, identified as erysovine and stachydrine, respectively. | 2013 | 1134218 |
| investigation of erythrina spp. iv. study of the chemical constituents of seeds of erythrina variegata var. orientalis. | | 1970 | 5484886 |