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a blood group a specific lectin from the seeds of crotalaria striata.a lectin, monospecific for human blood group a red blood cells was extracted from seeds of crotalaria striata and purified by molecular sieving on sephadex g-100 and ion-exchange on deae-cellulose. a molecular mass of 30 kda was determined by sds-polyacrylamide gel electrophoresis under non-reducing and reducing conditions. molecular sieving on a superose 12 column indicated a molecular mass of 110 kda, suggesting the tetrameric nature of the native protein. amino-acid composition showed the pre ...19902306467
lectins from seeds of crotalaria pallida (smooth rattlebox).a lectin from the seeds of crotalaria pallida (cpl), with an apparent molecular mass of 30 kda, determined by sds-polyacrylamide gel electrophoresis, showed human type a and b erythrocytes agglutination activity, which is inhibited by raffinose and galactose. the lectin requirement for divalent cation was demonstrated with edta/egta blocking hemagglutination activity. although the n-terminal amino acid sequence of cpl is identical to another lectin from crotalaria striata, which is taxonomically ...200212052508
intoxication of crotalaria pallida seeds to growing broiler chicks.the adverse effects of feeding crotalaria pallida (cp) seeds to chicks was investigated in a 21-day randomized trial of 4 dietary treatments (control, 1,2 and 3% ground cp seeds). mortality rates in birds fed 0, 1, 2, and 3% dietary cp were 0, 2.1, 6.2, and 16.7%, respectively. body weight gain and feed efficiency were adversely affected by all levels of inclusion of cp seeds, but feed intake was decreased only by dietary levels of 2 and 3%. dietary cp of 2 and 3% increased the relative weight o ...200312882487
effect of trypsin inhibitor from crotalaria pallida seeds on callosobruchus maculatus (cowpea weevil) and ceratitis capitata (fruit fly).a proteinaceous trypsin inhibitor was purified from crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin-sepharose and tca precipitation. the trypsin inhibitor, named cpati, had m(r) of 32.5 kda as determined by sds-page and was composed of two subunits with 27.7 and 5.6 kda linked by disulfide bridges. cpati was stable at 50 degrees c and lost 40% of activity at 100 degrees c. cpati was also stable from ph 2 to 12 at 37 degrees c. cpati weak ...200516426854
diverse bacteria isolated from root nodules of trifolium, crotalaria and mimosa grown in the subtropical regions of china.to analyze the diversity and relationships of rhizobia in the subtropical and tropical zones of china, we characterized 67 bacterial strains isolated from root nodules of five legume species in the genera trifolium, crotalaria and mimosa . pcr-amplified 16s rdna rflp, numerical taxonomy, sds-page of whole cell proteins, sequencing of 16s rdna and dna-dna hybridization grouped the isolates into 17 lineages belonging to bradyrhizobium, mesorhizobium, rhizobium, sinorhizobium and burkholderia, as w ...200717497134
a novel antimicrobial peptide from crotalaria pallida seeds with activity against human and phytopathogens.an actual severe problem in agriculture consists of an expressive increase of economical losses caused by fungi and resistant bacteria toward antibiotics. in order to find a solution to this problem, several studies have been concentrating on the screening of novel plant defense peptides with antimicrobial activities. these peptides are commonly characterized by having low molecular masses and cationic charges. the present work reports the purification and characterization of a novel plant pepti ...200919641962
effects of a novel pathogenesis-related class 10 (pr-10) protein from crotalaria pallida roots with papain inhibitory activity against root-knot nematode meloidogyne incognita.a novel pathogenesis-related class 10 (pr-10) protein with papain inhibitory activity, named cppri, was purified from crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (hplcs). cppri is made up of a single polypeptide chain with a m(r) of 15 kda, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this protein exhibited a k(i) value of 1.8 x 10(-9) m and operates via a noncompetit ...201020199085
estrogenic and mutagenic activities of crotalaria pallida measured by recombinant yeast assay and ames test.crotalaria pallida ailton is a plant belonging to the fabaceae family, popularly known as "rattle or rattlesnake" and used in traditional medicine to treat swelling of the joints and as a vermifuge. previous pharmacological studies have also reported anti-inflammatory, antimicrobial and antifungal activities. nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. thus, the purpose of the present st ...201324134316
population dynamics of dichelops melacanthus (dallas) (heteroptera: pentatomidae) on host plants.the stink bug dichelops melacanthus (dallas) has become one of the major pests of corn and wheat in brasil, mainly after a shift from the conventional tillage system to the no tillage cultivation system. this fact may be due to the simultaneous occurrence of second planting corn with wheat cultivation, and the presence of wild hosts. this study aimed to evaluate the population dynamics of d. melacanthus on wild hosts adjacent to areas cultivated with corn, wheat, and soybean during the season an ...201323949747
seed predation in the legume crotalaria : i. intensity and variability of seed predation in native and introduced populations of c. pallida ait.in order to evaluate the role of seed predators as selective agents within a population of conspecific plants, the intensity of predispersal seed predation by chewing insects and the variation in predation intensity with individual and season were measured for the legume shrub crotalaria pallida. in a native, tropical (east african) population, predation intensity averaged 49% for late dry season-early wet season samples, but ranged from less than 20% to near 100% for different individuals. aver ...197828309549
seed predation in the legume crotalaria : ii. correlates of interplant variability in predation intensity.correlates of individual plant escape from seed predation were investigated for an east african population of the shrub crotalaria pallida (papilionaceae) in which seed mortality due to chewing insects is high and varies among neighboring individuals. several plant properties expected to influence phytophagous insect host choice or fitness were measured simultaneously and their contribution to variance in predation examined with multiple regression analysis. microhabitat, seed pyrrolizidine alka ...197828309550
chemical modification studies on a blood group a-specific lectin, crotalarin (crotalaria striata) and its effect on hemagglutinating activity.crotalarin, the n-acetyl-d-galactosamine-binding blood group a-specific lectin from the seeds of crotalaria striata was subjected to various chemical modifications in order to ascertain the amino acid residues responsible for its carbohydrate-binding property. modification of lysine, cysteine and arginine residues did not affect the carbohydrate-binding activity of the lectin. however, modification of tyrosine residue and carboxy group of the acidic amino acids led to a complete loss of its acti ...19902177141
studies on the effect of various parameters on crotalarin-fetuin interaction.with a view to optimise the interaction of crotalarin, a blood group a-specific lectin from the seeds of crotalaria striata with fetuin the effect of various parameters on the reaction has been studied turbidimetrically. the formation of crotalarin-fetuin complex was dependent on time, temperature, ph and the ionic strength of the medium. the maximum turbidity appeared in 30 min at 20 degrees c and the ph optimum was 3.5. the binding constant (ka) for crotalarin-fetuin interaction was 5.58 x 10( ...19911715314
light perception for sun-tracking is on the lamina in crotalaria pallida (fabaceae).trifoliolate leaves of crotalaria pallida aiton (fabaceae) exhibit sun-tracking behavior in simulated days and in response to a fixed, oblique light. the site of light perception for sun-tracking is on the lamina, necessitating transport of a signal from the lamina to the site of response (the pulvinule at the base of the leaflet). evidence for the site of light perception on the lamina includes the following: (1) leaflet movement in response to oblique light is not affected by shading the pulvi ...199721708584
varying herbivore population structure correlates with lack of local adaptation in a geographic variable plant-herbivore interaction.local adaptation of parasites to their hosts due to coevolution is a central prediction of many theories in evolutionary biology. however, empirical studies looking for parasite local adaptation show great variation in outcomes, and the reasons for such variation are largely unknown. in a previous study, we showed adaptive differentiation in the arctiid moth utetheisa ornatrix to its host plant, the pyrrolizidine alkaloid-bearing legume crotalaria pallida, at the continental scale, but found no ...201122220208
common-garden experiments reveal geographical variation in the interaction among crotalaria pallida (leguminosae: papilionideae), utetheisa ornatrix l. (lepidoptera: arctiidae), and extrafloral nectary visiting ants.the study of geographical variation is a key approach to understand evolution of ecological interactions. we investigated geographical variation in the interaction among crotalaria pallida (leguminosae: papilionideae), its specialized herbivore, utetheisa ornatrix l. (lepidoptera: arctiidae), and ants attracted to extrafloral nectaries (efns). first, we used common-garden experiments with plants collected in different sites at different geographical scales to test for differences among populatio ...201323949803
effects of dietary crotalaria pallida seeds on the health and performance of laying hens and evaluation of residues in eggs.the effect of three dietary concentrations of crotalaria pallida (c. pallida) seeds (0, 1, 2, and 3% w/w) of their normal diet were investigated in commercial laying hens during a 35 day feeding trial. all concentrations of c. pallida decreased body weight and feed intake (p < 0.05). egg mass production and average egg weight were decreased by feeding of ≥ 2% c. pallida seeds (p < 0.05). all concentrations of c. pallida increased relative lung weight and serum activity of alt, ast and ldh (p < 0 ...201425062989
pyrrolizidine alkaloids negatively affect a generalist herbivore feeding on the chemically protected legume crotalaria pallida.plant secondary metabolites can have opposing effects on adapted specialist and non-adapted, generalist herbivores. in this study, we used heliothis virescens (fabricius) (lepidoptera: noctuidae) as a generalist, non-adapted model herbivore to test the possible effects of crotalaria pallida (fabaceae: papilionoideae) defenses on herbivore performance. neonate h. virescens larvae were able to consume c. pallida leaves and fruits and grow for a few instars, but none of them survived to pupation. w ...201626830432
phytoremediation of crude oil-contaminated soil employing crotalaria pallida aiton.the purpose of the study was to evaluate the phytoremediation potentiality of a herb named crotalaria pallida which are abundantly grown on crude oil-contaminated soil of oil field situated at upper assam, india, so that this plant could be used to remediate hydrocarbon from contaminated soil. to evaluate the potentiality of the plant, a pot culture experiment was conducted taking 3 kg of rice field soil mixed with crude oil at a concentration of 10,000 (10 g/kg), 20,000 (20 g/kg), 30,000 (30 g/ ...201626865490
anti-inflammatory constituents and new pterocarpanoid of crotalaria pallida.one new pterocarpanoid, crotafuran e (1), and three known compounds were isolated from the bark of crotalaria pallida. the structure of 1 was determined by spectral methods. two pterocarpanoids, crotafurans a (2) and b (3), previously isolated from this plant, showed significant concentration-dependent inhibitory effects on the no production in lipopolysaccharide (lps)-stimulated raw 264.7 macrophage-like cells, with ic(50) values of 23.0 +/- 1.0 and 19.0 +/- 0.2 microm, respectively. compound 3 ...200312662101
inhibition of lipopolysaccharide-stimulated no production by crotafuran b in raw 264.7 macrophages involves the blockade of nf-kappab activation through the increase in ikappabalpha synthesis.crotafuran b, a natural pterocarpanoid isolated from crotalaria pallida, inhibited the lipopolysaccharide (lps)-stimulated nitric oxide (no) production (ic50 16.4+/-0.7 microm) and inducible nitric oxide synthase (inos) protein and mrna expression (ic50 11.5+/-0.6 microm and 11.8+/-2.2 microm, respectively), but not via its cytotoxicity or the inhibition of inos enzyme activity, in raw 264.7 macrophages. crotafuran b also reduced the inos promoter activity (ic50 13.4+/-0.1 microm) in pinos-luc-t ...200616087206
anti-inflammatory flavonoids and pterocarpanoid from crotalaria pallida and c. assamica.one new isoflavone, 5,7,4'-trihydroxy-2'-methoxyisoflavone (3) and seven, and four known compounds were isolated from the barks of crotalaria pallida and the seeds of c. assamica, respectively. the known compounds, apigenin (1) and 2'-hydroxygenistein (2), isolated from c. pallida, showed significant concentration-dependent inhibitory effects on the release of beta-glucuronidase and lysozyme from rat neutrophils in response to formyl-met-leu-phe/cytochalasin b (fmlp/cb) with ic(50) values of 2.8 ...200415013012
multilocus sequences confirm the close genetic relationship of four phytoplasmas of peanut witches'-broom group 16srii-a.four witches'-broom diseases associated with arachis hypogaea (peanut), crotalaria pallida, tephrosia purpurea, and cleome viscosa were observed in hainan province, china during field surveys in 2004, 2005, and 2007. in previously reported studies, we identified these four phytoplasmas as members of subgroup 16srii-a, and discovered that their 16s rrna gene sequences were 99.9-100% identical to one another. in this study, we performed extensive phylogenetic analyses to elucidate relationships am ...201423686459
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