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isolation and fusion studies on protoplasts from pollen tetrads.different enzymes were tested for isolation of intact protoplasts from pollen tetrads. about 80% isolation was achieved from pollen tetrads of cajanus cajan and zea mays and about 60% from luffa cylindrica and lycopersicon esculentum after 4 h of treatment with 5% cellulase. when these mononucleate protoplasts were incubated in presence of 0.05 m cacl2 in 0.3 m glucose at ph 10.5, 70-80% fusion was achieved. fusion was rare in sodium nitrate solutions.1977321241
isolation and characterization of rhizobium (ic3342) genes that determine leaf curl induction in pigeon pea.nodulation by the rhizobium strain ic3342 causes a leaf curl syndrome in certain tropical legumes such as pigeon pea (cajanus cajan) (n.m. upadhyaya, j.v.d.k. kumar rao, d.s. letham, and p.j. dart, physiological and molecular plant pathology 39:357-373, 1991). transposon (tn5) mutagenesis of this leaf curl-inducing (curl+) rhizobium strain yielded two curl- fix- and three curl- fix+ mutants. plasmid visualization and subsequent southern blot hybridization analyses with tn5, nif and nod gene prob ...19921319772
hypolipidaemic activity of the protein isolated from cajanus cajan in high fat-cholesterol diet fed rats. 19734792951
a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk.eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ...19836615758
glycaemic response in normal subjects to five different legumes commonly used in the philippines.five legumes including chick pea (cicer arietinum linn), pigeon pea (cajanus cajan linn. huth.), black bean (phaseolus vulgaris linn), mung bean (phaseolus areus roxb) and white bean (phaseolus vulgaris linn) were cooked and tested for blood glucose response among healthy human volunteers. the blood glucose response to all legumes was significantly lower compared to bread. the glycaemic response to chick pea was significantly lower (p < or = 0.01) than that to black bean, pigeon pea and mung bea ...19957621088
abortive and teratogenic effect of acanthospermum hispidum dc. and cajanus cajan (l.) millps. in pregnant rats.extracts of acanthospermum hispidum and cajanus cajan have been used by brazilian people in an attempt to produce abortion. in order to evaluate the possible abortive and/or teratogenic effect of these plant extracts, female wistar rats were treated with the aqueous extract (infusion, proportion c. cajan and a. hispidum 1:1.3). doses of 0, 150, 300 and 600 mg/kg were daily administered by gavage during the organogenic period. the animals were sacrificed at term. there was no significant change i ...19947967648
amylase inhibitors of pigeonpea (cajanus cajan) seeds.pigeonpea (cajanus cajan l) seeds were analysed quantitatively for amylase inhibitor (ai) activity and qualitatively, by an in-gel-detection method on polyacrylamide gels. at least four ai isoforms were identified in pigeonpea seeds. the ais inhibit human salivary and bovine pancreatic amylase but fail to inhibit bacterial, fungal and endogenous amylase. pigeonpea ais were found to be active over a ph range of 4.5 to 9.5 and were heat labile. the isoelectric point of a major inhibitor is 6.2 ais ...19989431672
medicinal plants used for dogs in trinidad and tobago.this paper documents ethnoveterinary medicines used to treat dogs in trinidad and tobago. in 1995, a 4-stage process was used to conduct the research and document the ethnoveterinary practices. twenty-eight ethnoveterinary respondents were identified using the school-essay method, which is a modified rapid rural appraisal (rra) technique. semi-structured interviews were held with these respondents as well as with 30 veterinarians, 27 extension officers and 19 animal-health assistants and/or agri ...200010821961
indole acetic acid production by a rhizobium species from root nodules of a leguminous shrub, cajanus cajan.a rhizobium sp. isolated from the root nodules of a leguminous pulse-yielding shrub, cajanus cajan, was found to produce high amounts (99.7 microg/ml) of indole acetic acid (iaa) during growth in basal medium supplemented with l-tryptophan. the rhizobium sp. was a fast growing species which reached its stationary phase at 28 h. the iaa production could be increased upto 653.3% over control by supplementing the carbon-free incubation medium with glucose (5 g/l), nicl2 (10 microg/ml) and glutamic ...200010950195
purification and characterization of urease from dehusked pigeonpea (cajanus cajan l) seeds.urease has been purified from the dehusked seeds of pigeonpea (cajanus cajan l.) to apparent electrophoretic homogeneity with approximately 200 fold purification, with a specific activity of 6.24 x10(3) u mg(-1) protein. the enzyme was purified by the sequence of steps, namely, first acetone fractionation, acid step, a second acetone fractionation followed by gel filtration and anion-exchange chromatographies. single band was observed in both native- and sds-page. the molecular mass estimated fo ...200212409017
effect of protein fractions from cajanus cajan (redgram) and dolichos biflorus (horsegram) on the serum, liver and aortic lipid levels in rats fed a high-fat-cholesterol diet. 19734751222
characterization of a proteinase inhibitor from cajanus cajan (l.).a protein proteinase inhibitor (pi) has been purified from pigeonpea cajanus cajan (l.) pusa 33 variety by acetic-acid precipitation, salt fractionation and chromatography on a deae-cellulose column. the content of inhibitor was found to be 15 mg/20 g dry weight of pulse. the molecular weight of the inhibitor as determined by sds-page under reducing conditions was found to be about 14,000. it showed inhibitory activity toward proteolytic enzymes belonging to the serine protease group, namely try ...200314703988
effects of ddt on the growth of crop plants.the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ...198915092369
expression of hemagglutinin protein of rinderpest virus in transgenic pigeon pea [cajanus cajan (l.) millsp.] plants.rinderpest virus is the causative agent of a devastating, often fatal disease in wild and domestic bovids that is endemic in africa, the middle east and south asia. the existing live attenuated vaccine is heat-labile, and thus there is a need for the development of new strategies for vaccination. this paper reports the development of transgenic pigeon pea [ cajanus cajun (l.) millsp.] expressing one of the protective antigens, the hemagglutinin (h) protein of rinderpest virus. a 2-kb fragment co ...200312789415
partial 16s rrna gene sequence diversity and numerical taxonomy of slow growing pigeonpea (cajanus cajan l millsp) nodulating rhizobia.an investigation was carried out to determine the diversity of 30 isolates of slow growing pigeonpea nodulating rhizobia based on variations in partial sequences of the 16s rrna gene and numerical analysis of 80 phenotypic traits. phylogenetic analysis using molecular sequences of 23 isolates showed that arpe1 separated from the other isolates at an average distance of >14% divergence level. the other isolates were all within 5% divergence from each other but separated into four main groups, wit ...200212435494
panzee, a copia-like retrotransposon from the grain legume, pigeonpea ( cajanus cajan l.).we report the isolation and characterization of a copia-like retrotransposon, panzee, from pigeonpea ( cajanus cajan). the 4947-bp panzee element is at rich (60%) and the integrated element is flanked by a target-site duplication of 5 bp. the structure of panzee is that of a typical ltr-retrotransposon containing long terminal repeats (ltrs) which flank its internal region. the 5' ltr is 372 bp in length and the 3' ltr is 383 bp long. both ltrs start with 5'-tg and end with ca-3' and have 4-bp t ...200212073029
medicinal plants of india with anti-diabetic potential.since ancient times, plants have been an exemplary source of medicine. ayurveda and other indian literature mention the use of plants in treatment of various human ailments. india has about 45000 plant species and among them, several thousands have been claimed to possess medicinal properties. research conducted in last few decades on plants mentioned in ancient literature or used traditionally for diabetes have shown anti-diabetic property. the present paper reviews 45 such plants and their pro ...200212020931
purification of cajanus cajan root lectin and its interaction with rhizobial lipopolysaccharide as studied by different spectroscopic techniques.a lectin present in roots of cajanus cajan seedlings was isolated and purified by affinity chromatography. sugar specificity assayed by hemagglutination-inhibition activity indicated that lectin belongs to glucose/mannose-specific group. the root lectin was found to be mannose-specific from the second day onwards as it was reconfirmed by specific elution of different days' sample from mannose agarose matrix. the maximum interaction of lectin with goat igm was obtained in 10-day-old sample, indic ...200111716467
sinorhizobium fredii hh103 mutants affected in capsular polysaccharide (kps) are impaired for nodulation with soybean and cajanus cajan.the sinorhizobium fredii hh103 rkp-1 region, which is involved in capsular polysaccharides (kps) production, was isolated and sequenced. the organization of the s. fredii genes identified, rkpuaghij and kpsf3, was identical to that described for s. meliloti 1021 but different from that of s. meliloti ak631. the long rkpa gene (7.5 kb) of s. fredii hh103 and s. meliloti 1021 appears as a fusion of six clustered ak631 genes, rkpabcdef. s. fredii hh103-rif(r) mutants affected in rkph or rkpg were c ...200616404952
effect of soaking, cooking and germination on the oligosaccharide content of selected nigerian legume seeds.the identity and quantity of and effect of processing on raffinose oligosaccharides in raw, mature seeds of lima beans (phaseolus lunatus), pigeon peas (cajanus cajan), african yam beans (sphenostylis sternocarpa) and jackbeans (canavalia ensiformis) were investigated. sucrose, raffinose, stachyose and verbascose were identified by hplc in all the legume seeds. the total alpha-galactoside contents of the seeds in decreasing order were african yam beans 3.84 mg/100 mg; white lima beans 3.62 mg/10 ...200010898479
isolation and characterization of nodulation genes from bradyrhizobium sp. (vigna) strain irc 78.an 11.76-kilobase-pair (kb) segment of dna from bradyrhizobium sp. (vigna) strain irc 78 that hybridizes to nodulation genes of rhizobium meliloti strain 41 was isolated. hybridization of the 11.76-kb dna fragment to dna from other bradyrhizobium species revealed a high degree of sequence conservation in this region. transfer of the 11.76-kb segment to nodulation-defective (nod(-)) mutants of r. meliloti restored their ability to induce nodules on medicago sativa (alfalfa). mutants of strain irc ...198516593624
mutation in gdp-fucose synthesis genes of sinorhizobium fredii alters nod factors and significantly decreases competitiveness to nodulate soybeans.we mutagenized sinorhizobium fredii hh103-1 with tn5-b20 and screened about 2,000 colonies for increased beta-galactosidase activity in the presence of the flavonoid naringenin. one mutant, designated svq287, produces lipochitooligosaccharide nod factors (lcos) that differ from those of the parental strain. the nonreducing n-acetylglucosamine residues of all of the lcos of mutant svq287 lack fucose and 2-o-methylfucose substituents. in addition, svq287 synthesizes an lco with an unusually long, ...199910065558
fenvalerate residues in green pods, pod cover, and grain of pigeonpea (cajanus cajan l.). 19989484570
laboratory screening of different bacillus thuringiensis strains against certain lepidopteran pests and subsequent field evaluation on the pod boring pest complex of pigeonpea (cajanus cajan).laboratory evaluation of different bacillus thuringiensis subspecies revealed that b. thuringiensis subsp. kurstaki (ncim 2514) at 10(8) spores/ml concentration caused more than 85% mortality to the neonate larvae of the lepidopteran insects spodoptera litura (f.) and phthorimaea operculella (z.). this strain at 10(10) spores/ml concentration was effective against the major lepidopteran pests comprising the pod boring complex of pigeonpea (cajanus cajan), viz. helicoverpa armigera (h.) and exela ...19979195006
insect odour perception: recognition of odour components by flower foraging moths.odours emitted by flowers are complex blends of volatile compounds. these odours are learnt by flower-visiting insect species, improving their recognition of rewarding flowers and thus foraging efficiency. we investigated the flexibility of floral odour learning by testing whether adult moths recognize single compounds common to flowers on which they forage. dual choice preference tests on helicoverpa armigera moths allowed free flying moths to forage on one of three flower species; argyranthemu ...200616846910
protein quality of some nigerian traditional diets based on the african yambean (sphenostylis stenocarpa) and pigeon pea (cajanus cajan).the protein quality of nigerian traditional diets based on the african yambean (ayb) and pigeon pea (pp) were performed by in vivo and in vitro bioassays. the individual foods were processed, cooked, dried and mixed to resemble the traditional diets. weanling male wistar rats (45-55 g) were used for the bioassay. the rats were fed ad libitum for 35 days of which 28 days were for growth and 7 days for n-balance. all the eleven diets including casein (control) provided 1.6 g n/100 g diet. the para ...19958882368
production of l-asparaginase, an anticancer agent, from aspergillus niger using agricultural waste in solid state fermentation.this article reports the production of high levels of l-asparaginase from a new isolate of aspergillus niger in solid state fermentation (ssf) using agro-wastes from three leguminous crops (bran of cajanus cajan, phaseolus mungo, and glycine max). when used as the sole source for growth in ssf, bran of g. max showed maximum enzyme production followed by that of p. mungo and c. cajan. a 96-h fermentation time under aerobic condition with moisture content of 70%, 30 min of cooking time and 1205-14 ...200617057254
functional expression of escherichia coli fhua gene in rhizobium spp. of cajanus cajan provides growth advantage in presence of fe3+: ferrichrome as iron source.cajanus cajan rhizobial isolates were found to be unable to utilize iron bound to ferrichrome, desferrioxamine b or rhodotorulic acid, all being hydroxamate type siderophores. a broad host range expression vector containing the escherichia coli fhua gene, encoding the outer membrane receptor for fe-ferrichrome, was constructed. the plasmid construct (pgr1), designed to express fhua under the lac promoter of e. coli, complemented e. coli mb97 deltafhua mutant for ferri-ferrichrome utilization and ...200717136381
antileishmanial and antifungal activity of plants used in traditional medicine in brazil.the antileishmanial and antifungal activity of 24 methanol extracts from 20 plants, all of them used in the brazilian traditional medicine for the treatment of several infectious and inflammatory disorders, were evaluated against promastigotes forms of two species of leishmania (l. amazonensis and l. chagasi) and two yeasts (candida albicans and cryptococcus neoformans). among the 20 tested methanolic extracts, those of vernonia polyanthes was the most active against l. amazonensis (ic(50) of 4 ...200717234373
content of antinutrients and in vitro protein digestibility of the african yambean, pigeon and cowpea.the protein, trypsin inhibitor (ti), tannin, phytate, phytic acid phosphorus and in vitro protein digestibility (ivpd) of cultivars of the african yambean (sphenostylis stenocarpa) - ayb, pigeon pea (cajanus cajan) - pp and cowpea (vigna unguiculata) - cp were determined. the protein content of cp (24-28.0%) was higher than those of pp (21-22.5%) and ayb (21-22.5%). the cream and speckled ayb contained more ti (30.9 and 25.3 mg/g) than pp (7.5-14.1 mg/g) and cp (9.8-20.5 mg/g). apart from the wh ...19958833429
bioformulation of burkholderia sp. mssp with a multispecies consortium for growth promotion of cajanus cajan.the present work was undertaken to formulate an effective bioformulation using burkholderia sp. strain mssp, a known plant-growth-promoting rhizobacterium. mssp was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane-bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer-whey as liquid carrier. physical and chemical properties of different low-cost carrier materials ...200717496969
mycoflora and aflatoxin production in pigeon pea stored in jute sacks and iron bins.the mycoflora, moisture content and aflatoxin contamination of pigeon pea (cajanus cajan (l.) millisp) stored in jute sacks and iron bins were determined at monthly intervals for a year. the predominant fungi on freshly harvested seeds were alternaria spp., botryodiplodia theobromae, fusarium spp. and phoma spp. these fungi gradually disappeared from stored seeds with time and by 5-6 months, most were not isolated. the fungi that succeeded the initially dominant ones were mainly members of the g ...19958684429
effect of dietary protein quality on rat kidney glutaminase activity.kidneys of rats fed for 10 days a diet containing protein from cajanus cajan were found to show an increase in the maleate-induced phosphate-independent glutaminase (pig) activity as compared to the rats fed a diet containing egg protein. these changes could be reversed by reversing the diets. pig is known to possess the catalytic function of gamma-glutamyl transpeptidase, which showed parallel changes. starvation of adult rats for 24 h as well as the growth of young rats into adults resulted in ...19816116475
studies on the nutritive values of cow pea (vigna catjang) and tur dhal (cajanus cajan). 19676068885
antiplasmodial constituents of cajanus cajan.bioactivity-guided fractionation of extracts of roots and leaves of cajanus cajan afforded 8 compounds: betulinic acid, biochanin a, cajanol, genistein and 2'-hydroxygenistein, longistylin a and c, and pinostrobin. the two stilbenes, longistylin a and c, and betulinic acid showed a moderately high in vitro activity against the chloroquine-sensitive plasmodium falciparum strain 3d7.200415022164
declination of copper toxicity in pigeon pea and soil system by growth-promoting proteus vulgaris knp3 strain.the copper-resistant (1318 microm cuso(4).5h(2)o) strain knp3 of proteus vulgaris was isolated from soil near the panki power plant, kanpur, india, and was used to inoculate pigeon pea (cajanus cajan var. ups-120) seeds grown in soil for 60 days in the presence of 600 microm cuso(4).5h(2)o. a study of siderophore production (126.34 +/- 0.52 microg ml(-1)) and its subsequent effects on plant growth promotion under in situ conditions was conducted. the parameters that were monitored included the p ...200818392891
effect of dietary protein quality on the serum enzymes of rats exposed to simulated hypobaric hypoxic stress.changes in dietary protein quality and high altitude stress alter the serum urea cycle enzyme activities. to find a dietary protein which will facilitate quicker acclimatization to altitude stress, groups of rats were fed two diets, isocaloric and isonitrogenous, but differing in amino acid make up. the rats were exposed to simulated altitude stress of 0.5 atmospheric pressure for various periods up to 65 h. when serum arginase and ornithine transcarbamylase activities were assayed, it was seen ...19863800815
hepatic and serum arginase and ornithine transcarbamylase activities of rats maintained on diets of different protein quality.groups of rats were fed diets containing 15% protein from arhar dal (cajanus cajan), bengal gram dal (cicer arietinum), urad dal (phaseolus mungo) and also isolated proteins from casein, egg, soya bean, gluten and gelatin for a period of 10 days. rats maintained on the casein diet had the highest liver arginase activity and those having egg the lowest. all the leguminous proteins gave similar levels of arginase activity, the values falling between the casein and egg groups. serum arginase level ...19873426155
a sensitive and rapid immunochemical method for quantitation of proteins.a sensitive and rapid elisa for quantitation of seed globulins is described. this method employs conjugation of pigeon pea (cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. using this conjugate, proteins as low as 0.1 ng were detected. dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit igg conjugate, and is three-fold more efficient than pigeon pea globulin igg peroxidase conjugate. the ...19883233341
hch, endosulfan, and fluvalinate residue behavior in pigeonpea (cajanus cajan l. millsp). 19921374672
induced isoflavonoids from fungus-infected stems of pigeon pea (cajanus cajan).four antifungal isoflavones [7-hydroxy-4'-methoxy-(1); 5,7,4'-trihydroxy-(2); 5,7,2',4'-tetrahydroxy-(3); 5,2',4'-trihydroxy-7-methoxy-(4)] and one isoflavanone [(5,2'-dihydroxy-7,4'-dimethoxy-(7)] have been isolated from the fungus-infected stems of cajanus cajan. a sixth compound has been provisionally identified as 5,2'-dihydroxy-7,4'-dimethoxyisoflavone (5). the structure of 5,2'-dihydroxy-7,4'-dimethoxyisoflavanone (cajanol) was confirmed by synthesis from ferreirin (5,7,2'-trihydroxy-4'-me ...1976136119
cover crops and natural vegetation mulch effect achieved by mechanical management with lateral rotary mower in weed population dynamics in citrus.the aim of this study was to obtain information on practical weed management in order to reduce the use of herbicides thereby contributing to the sustainable development of citrus crop. the experiment was carried out under field conditions at the experimental area of the department of vegetal production at the college of agriculture "luiz de queiroz," piracicaba, sp, brazil, during the season 2002-2003. influence of mulches produced by four types of vegetations on the dynamic population of weeds ...200515656179
mutation in the cobo gene generates auxotrophy for cobalamin and methionine and impairs the symbiotic properties of sinorhizobium fredii hh103 with soybean and other legumes.we report here the isolation of a methionine and cobalamin mutant strain (svq336) of sinorhizobium fredii hh103 obtained by tn5-lacz mutagenesis. sequence analysis showed that the transposon was inserted into a gene homologous to cobo. this gene codes for a cobalamin adenosyltransferase which is involved in the biosynthesis of vitamin b12. another hh103 cobo mutant (strain svq524), was constructed by the insertion of omega interposon. both cobo mutants required the addition of methionine because ...200918719891
isolation and characterization of a green tissue-specific promoter from pigeonpea [cajanus cajan (l.) millsp.].expression of rbcs genes encoding small subunit of rubisco, most abundant protein in green tissue, is regulated by at least three parameters--tissue type, light conditions and stage of development. one of the green tissue-specific promoters of rbcs gene family was isolated from pigeonpea by pcr. expression of uida gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcs promoter, clearly showed that this promoter was as strong as pea rbcs3a promoter c ...200515875723
host range nodulation and adaptation in frenchbean rhizobia.the host range nodulation efficiency of four genetically marked frenchbean rhizobial strains (hurr-3, raj-2, raj-5 and raj-6) was studied with five legume hosts namely, frenchbean (phageolus vulgaris l.), pigeonpea [cajanus cajan (l.) millsp.], mungbean [vigna radiata (l.) wilezek.], urdbean [vigna mungo (l.) hepper.] and soybean [glycine max (l.) merril.]. except soybean and pigeonpea, all other legume hosts were nodulated by two or more frenchbean rhizobial strains tested. rhizobia were isolat ...200616538866
optimum domestic processing and cooking methods for reducing the polyphenolic (antinutrient) content of pigeon peas.four high yielding and early maturing cultivars of pigeon pea (cajanus cajan) namely upas-120, icpl-87, icpl-151 and, especially, manak, contained significant amounts of polyphenols (1075 to 1328 mg/100g), which may limit their utilization. the effectiveness of soaking (6, 12 and 18 h, 30 degrees c), soaking and dehulling, ordinary cooking, pressure cooking and germination (24, 36, 48 h, 30 degrees c) in reducing the levels of polyphenols was investigated. a decrease in the polyphenolic contents ...200010768410
chemotaxis of rhizobium sp.s2 towards cajanus cajan root exudate and its major components.the chemotactic response of rhizobium sp. s2, a slow-growing cajanus cajan isolate, towards its host root exudate was examined. two classes of mutants, one nonchemotactic towards nutrients (amino acids and sugars) and signal compounds like flavonoids and the other, nonchemotactic towards amino acids and sugars but positive towards naringenin, the flavonoid present in cajanus cajan root exudate, were obtained. the plasmid-cured derivative of the parent showed positive response towards amino acids ...199910069855
evidence for cytokinin involvement in rhizobium (ic3342)-induced leaf curl syndrome of pigeonpea (cajanus cajan millsp.).a uniquely abnormal shoot development (shoot tip-bending, leaf curling, release from apical dominance, and stunted growth) in pigeonpea (cajanus cajan millsp) induced by a nodulating rhizobium strain, ic3342, is thought to be due to a hormonal imbalance. amaranthus betacyanin bioassay indicated that xylem exudate and leaf extracts from pigeonpea plants with rhizobium-induced leaf curl symptoms contained high concentrations of cytokinin relative to those in normal plants. radioimmunoassay (ria) o ...199116668086
protein quality of pigeonpea (cajanus cajan (l.) millsp.) as influenced by seed polyphenols and cooking process.true protein digestibility (td), biological value (bv), and net protein utilization (npu) of low-polyphenol pigeonpea cultivars (nylon, bdn 2, and icpl 87067) were significantly higher than those of the high polyphenol cultivars (c 11, icpl 87, and icpl 151) when whole-seed samples were compared. most of the polyphenols (80-90%) were concentrated in the seed-coat. dhal (decorticated split cotyledons) samples of low (nylon) and high (c 11) polyphenol cultivars revealed no large differences in td, ...19938386359
compositional evaluation of some cowpea varieties and some under-utilized edible legumes in nigeria.the nutritive potentials of some cowpea varieties such as ife brown, ife bimpe, it84e-124, k59 and tvx716 and some under-utilized edible legumes grown in nigeria such as pigeon pea (cajanus cajan), lima bean (phaseolus lunatus), lablab bean (dolichos lablab), mucuna bean (mucuna sp.) and sphenostilis sternocarpa have been evaluated with respect to their proximate chemical composition, mineral content and some endogenous toxic constituents. the cowpea varieties contained on the average 22.5 g cru ...19892561305
limiting an insect infestation of nitrogen-fixing root nodules of the pigeon pea (cajanus cajan) by engineering the expression of an entomocidal gene in its root nodules.a region of dna which determined the production of the insecticidal toxin of bacillus thuringiensis subsp. israelensis was cloned into a derivative of a broad-host-range group incq plasmid vector of gram-negative bacteria. the plasmid which we constructed was transferred by conjugative mobilization into a bradyrhizobium species that nodulates pigeon peas. in this species the construction was maintained stably in the absence of selection and expressed the gene that was installed. experiments in a ...199016348294
cluster bean-a ureide- or amide-producing legume?xylem sap of cluster bean (cyamopsis tetragonoloba l. cv fs-277) and pigeonpea (cajanus cajan cv upas-120) were analyzed for total nitrogen, amide nitrogen, and ureide nitrogen at flowering stage. nitrogenase, uricase, and allantoinase were compared in nodules of cluster bean and pigeonpea. xylem sap of cluster bean exhibited higher amounts of amides as compared to ureides, and the activities of uricase and allantoinase (ureide-producing enzymes) in nodules were also low, whereas the reverse was ...198216662600
pigeonpea (cajanus cajan l. millsp.).pigeonpea [cajanus cajan (l.) millsp.], also known as redgram, is one of the major grain legume (pulses) crops grown in the semiarid tropics (sat) extending between 30 degrees n and 30 degrees s; it is the second most important food legume of india. it is cultivated in about 50 countries of asia, africa, and the americas for a variety of uses (food, fodder, fuel wood, rearing lac insects, hedges, wind breaks, soil conservation, green manure, roofing, and so on). the constraints of enhancing its ...200616988359
ethnobotanical survey and cytotoxicity testing of plants of south-western nigeria used to treat cancer, with isolation of cytotoxic constituents from cajanus cajan millsp. leaves.there is only scant literature on the anticancer components of medicinal plants from nigeria, yet traditional healers in the area under study claim to have been managing the disease in their patients with some success using the species studied.201020064598
inhibitors from pigeonpea active against lepidopteran gut proteinases.the proteinase inhibitors (pis) active against bovine pancreatic trypsin, chymotrypsin, and insect midgut trypsin-like proteinases were found in the seeds of 14 cultivars and eight wild types of pigeonpea, cajanus cajan l.. the inhibitory activity of pis against trypsin and chymotrypsin, as well as their activity profile on gelatin-polyacrylamide gel electrophoresis (page) were identical among the various cultivars. in contrast to cultivars, the wild types showed differences in inhibitory activi ...200920069866
[effects of the extracts of cajanus cajan l. on cell functions in human osteoblast-like te85 cells and the derivation of osteoclast-like cells].the cajanine (longistylin a-2-carboxylic acid) is isolated and identified from extracts of cajanus cajan l. (ecc) , which structure is similar to diethylstilbestrol. the regulation properties of the cajanine and other four extracts of cajanus cajan l. (32-1, 35-1, 35-2, and 35-3) were tested in human osteoblast-like (hos) te85 cells and marrow-derived osteoclast-like cells. by using mtt assay to test the change of cell proliferation, 3h-proline incorporation to investigate the formation of colla ...200717633205
enhanced growth and nodulation of pigeon pea by co-inoculation of bacillus strains with rhizobium spp.endophytic bacteria which are known to reside in plant tissues have often been shown to promote plant growth. present study deals with the isolation of putative endophytes from the surface sterilized root nodules of pigeon pea (cajanus cajan) designated as non-rhizobial (nr) isolates. three of these non-rhizobial isolates called nr2, nr4 and nr6 showed plant growth promotion with respect to increase in plant fresh weight, chlorophyll content, nodule number and nodule fresh weight when co-inocula ...200817826983
comparative study on the antiherpetic activity of aqueous and ethanolic extracts derived from cajanus cajan (l.) millsp.aqueous and ethanolic extracts of cajanus cajan (fabaceae) were examined in vitro for their antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2).201020215758
improved and convenient method of rna isolation from polyphenols and polysaccharide rich plant tissues.it has been difficult to extract a good quality total rna from the plant parts (such as seeds) which contain high levels of phenolic compounds, carbohydrates and other compounds that bind and/or co-precipitate with rna. a simple, rapid and efficient method for isolating total rna from polyphenols and polysaccharide rich plant tissues has been developed. seeds of leguminosae family were chosen for the study. the good quality and high yield of total rna was achieved with a260/a280 ratio of 1.9. se ...200819245182
chemical composition of the sfe-co extracts from cajanus cajan (l.) huth and their antimicrobial activity in vitro and in vivo.the in vitro and in vivo antimicrobial activities of sfe-co₂(supercritical fluid extraction) extracts and ethanol extracts from cajanus cajan (l.) huth were investigated. the flavonoid compounds orientin, vitexin, isovitexin, pinostrobin and the stilbene cajaninstilbene acid were detected in sfe-co₂ extracts by hplc-dad. in vitro antimicrobial activities of the extracts were evaluated against eight microbial strains (the bacteria staphylococcus epidermidis, staphylococcus aureus, bacillus subtil ...201020576412
partial characterization of red gram (cajanus cajan l. millsp) polypeptides recognized by patients exhibiting rhinitis and bronchial asthma.we sought to assess the allergenic potential of red gram by identifying and characterizing the responsible proteins. immunoblotting was performed to detect ige binding proteins. identities of these proteins were confirmed by mass spectrometry. to evaluate allergenic potential, balb/c mice were sensitized with red gram proteins and levels of specific immunoglobulins, histamine, th2 cytokines were measured. allergenic response was evident by significant increase in specific ige, igg1, histamine an ...201020600518
potential of carbon accumulation in no-till soils with intensive use and cover crops in southern brazil.the area under no-till (nt) in brazil reached 22 million ha in 2004-2005, of which approximately 45% was located in the southern states. from the 1970s to the mid-1980s, this region was a source of carbon dioxide to the atmosphere due to decrease of soil carbon (c) stocks and high consumption of fuel by intensive tillage. since then, nt has partially restored the soil c lost and reduced the consumption of fossil fuels. to assess the potential of c accumulation in nt soils, four long-term experim ...200616825480
[hypocholesterolemic effect of stilbene extract from cajanus cajan l. on serum and hepatic lipid in diet-induced hyperlipidemic mice].cajanus cajan l. is a natural plant, which contains a lot of potential active components. in the present study, we identified the effects of the stilbene extract from cajanus cajan l. (secc) on hepatic cholesterol metabolism in diet-induced (for 4 weeks) hyperlipidemic kunming mice. all experimental mice were divided into 5 groups: control group, high lipid model group, secc-treated with 200 or 100 mg kg(-1), and simvastatin (sim, 12 mg kg(-1)) treated group. the mice were fed with fat and chole ...200818507340
increased effectiveness of competitive rhizobium strains upon inoculation of cajanus cajan.a field study was conducted in lysimeters containing n-enriched soil to determine the effects of four competitive rhizobium strains upon yield parameters of pigeon peas (cajanus cajan). the greatest differences observed were in seed yields; strain p132 effected the highest seed yield (121 +/- 20 g per plant), and the control strain (indigenous rhizobia) effected the lowest yield (43.9 +/- 8 g per plant). with the exception of seeds and pods, the dry matter weights were not different. although th ...198716347429
cajanol inhibits the growth of escherichia coli and staphylococcus aureus by acting on membrane and dna damage.in the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of cajanus cajan (l.) millsp. towards escherichia coli (e. coli) and staphylococcus aureus (s. aureus) was investigated. the antibacterial activity of cajanol was evaluated towards six bacterial strains (staphylococcus epidermidis, staphylococcus aureus, bacillus subtilis, escherichia coli, proteus vulgaris, and pseudomonas aeruginosa) by the broth microdilution method. it showed strong antibacteria ...201120803417
biological control of fusarium wilt of pigeonpea cajanus cajan (l.) millsp with chitinolytic alcaligenes xylosoxydans.alcaligenes xylosoxydans protected pigeonpea from fusarium wilt in a pot experiment and field trials. when seeds of pigeonpea (c. cajan) were treated with a. xylosoxydans and sown in soil infested with fusarium, the incidence of wilt was reduced by 43.5% and resulted in 58% higher grain yield. the antifungal activity of a. xylosoxydans was based on chitinase production and was comparable in efficacy to commercial antifungal agents such as benlate, monitor wp, thiram and bavistin.200315320506
regulation of population densities of heterodera cajani and other plant-parasitic nematodes by crop rotations on vertisols, in semi-arid tropical production systems in india.the significance of double crop (intercrop and sequential crop), single crop (rainy season crop fallow from june to september), and rotations on densities of heterodera cajani, helicotylenchus retusus, and rotylenchulus reniformis was studied on vertisol (typic pellusterts) between 1987 and 1993. cowpea (vigna sinensis), mungbean (phaseolus aureus), and pigeonpea (cajanus cajan) greatly increased the population densities of h. cajani and suppressed the population densities of other plant-parasit ...199619277141
reaction of pigeonpea cultivars and germplasm accessions to the root-knot nematode, meloidogyne javanica.meloidogyne javanica is an important nematode pest of pigeonpea. thirty-four pigeonpea cultivars and 227 germplasm accessions were evaluated for resistance to m. javanica based on number of galls, egg masses, size of galls, and area of root covered with galls. galls were not formed on 75% of the cultivars, and no egg masses were observed on the roots of four cultivars (upas 120, pant a3, co 1, and bdn 2); however, shoot mass of 64% of the cultivars was reduced by m. javanica. pant a3, anm 504, a ...199419279941
in vivo evaluation of the antiviral activity of cajanus cajan on measles virus.cajanus cajan, a tropical shrub, serves as source of food and traditional medicines. the evaluation of aqueous and ethanol extracts for activity against measles virus and toxicity to embryonated chicken eggs was carried out in this study. in vivo and in vitro assay techniques using embryonated chicken eggs and tissue culture (hep-2 cell lines) as media for both virus cultivation and anti-virus assay showed that a hot-water extract yielded higher activity against measles virus. the hot-water extr ...201121614435
constituents of cajanus cajan (l.) millsp., moringa oleifera lam., heliotropium indicum l. and bidens pilosa l. from nigeria.the essential oils of four plant species from nigeria have been extracted by hydrodistillation and analyzed by gc and gc-ms. the oils of cajanus cajan were comprised of sesquiterpenes (92.5%, 81.2% and 94.3% respectively in the leaves, stem and seeds). the major compounds identified were alpha-himachalene (9.0-11.5%), beta-himachalene (8.0-11.0%), gamma-himachalene (6.9-8.1%), alpha-humulene (7.1-8.7%) and alpha-copaene (4.5-5.6%). however, monoterpenoid compounds (81.8%) dominated the oil of mo ...200919476009
influence of heat processing on the bioaccessibility of zinc and iron from cereals and pulses consumed in india.influence of heat processing on the bioaccessibility of zinc and iron from food grains consumed in india was evaluated. cereals - rice (oryza sativa), finger millet (eleusine coracana), sorghum (sorghum vulgare), wheat (triticum aestivum), and maize (zea mays), and pulses - chickpea (cicer arietinum) - whole and decorticated, green gram (phaseolus aureus) - whole and decorticated, decorticated black gram (phaseolus mungo), decorticated red gram (cajanus cajan), cowpea (vigna catjang), and french ...200717317519
[effects of the stilbene extracts from cajanus cajan l. on ovariectomy-induced bone loss in rats].the cajanus cajan l. is a natural plant, which contains lots of potential active components. the effects of the stilbene extracts from cajanus cajan l. (secc) on ovariectomy (ovx)-induced bone loss in rats were identified. all experimental female rats were divided into 6 groups, i. e. sham-operated rats, ovx rats, 17beta-estradiol (e2)-treated rats, secc-treated rats with three dosages, 50, 100, and 200 mg x kg(-1), separately. two weeks after the operation, different dosage of secc, e2 or deion ...200717703784
a pyrf auxotrophic mutant of sinorhizobium fredii hh103 impaired in its symbiotic interactions with soybean and other legumes.transposon tn5-mob mutagenesis allowed the selection of a sinorhizobium fredii hh103 mutant derivative (svq 292) that requires the presence of uracil to grow in minimal media. the mutated gene, pyrf, codes for an orotidine-5 - monophosphate decarboxylase (ec 4.1.1.23). mutant svq 292 and its parental prototrophic mutant hh103 showed similar nod-factor and lipopolysaccharide profiles. the symbiotic properties of mutant svq 292 were severely impaired with all legumes tested. mutant svq 292 formed ...200718075998
hypocholesterolemic effect of stilbenes containing extract-fraction from cajanus cajan l. on diet-induced hypercholesterolemia in mice.cajanus cajan (l) is a natural plant which contains a lot of potential active components. in the present study, we identified the effects of the stilbenes containing extract-fraction from cajanus cajan l (secc) on diet-induced (for 4 weeks) hypercholesterolemia in kunming mice. all experimental mice were divided into 5 groups: control group, model group, secc-treated with 200 or 100mg/kg/day, and simvastatin group. the effects of secc were investigated by monitoring serum and liver lipid profile ...200818420398
cajanus cajan linn. (leguminosae) prevents alcohol-induced rat liver damage and augments cytoprotective function.cajanus cajan linn. (leguminosae) is a nontoxic edible herb, widely used in indian folk medicine for the prevention of various liver disorders. in the present study we have demonstrated that methanol-aqueous fraction (maf2) of cajanus cajan leaf extract could prevent the chronically treated alcohol induced rat liver damage.200818584980
purification, crystallization and preliminary x-ray analysis of urease from pigeon pea (cajanus cajan).urease is a seed protein that is common to most leguminosae. it also occurs in many bacteria, fungi and several species of yeast. urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide, thus allowing organisms to use exogenous and internally generated urea as a nitrogen source. urease from pigeon pea seeds has been purified to electrophoretic homogeneity using a series of steps involving ammonium sulfate fractionation, acid precipitation, ion-exchange and size-exclusion chromatogr ...200818607103
development of genic-ssr markers by deep transcriptome sequencing in pigeonpea [cajanus cajan (l.) millspaugh].pigeonpea [cajanus cajan (l.) millspaugh], one of the most important food legumes of semi-arid tropical and subtropical regions, has limited genomic resources, particularly expressed sequence based (genic) markers. we report a comprehensive set of validated genic simple sequence repeat (ssr) markers using deep transcriptome sequencing, and its application in genetic diversity analysis and mapping.201121251263
bowman-birk proteinase inhibitor from cajanus cajan seeds: purification, characterization, and insecticidal properties.a red gram proteinase inhibitor (rgpi) was purified from red gram ( cajanus cajan ) seeds by using ammonium sulfate precipitation and ion-exchange, affinity, and gel filtration chromatography. sds-page under nonreducing condition revealed two protein bands with molecular masses of approximately 8.5 and approximately 16.5 kda corresponding to monomeric and dimeric forms of rgpi, respectively. similarly, matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry also ...201020146519
intake, digestibility, and nitrogen retention by sheep supplemented with warm-season legume hays or soybean meal.the increasing cost of feed supplements necessitates evaluation of alternatives for ruminant livestock grazing poor quality warm-season grasses. this study determined how supplementing bahiagrass hay (paspalum notatum flügge cv. pensacola) with soybean [glycine max (l.) merr.] meal or warm-season legume hays affected intake, digestibility, and n utilization by lambs. dorper x katadhin crossbred lambs (30.6 +/- 5.5 kg; n = 42) were fed bahiagrass hay (73.8% ndf, 8.1% cp) for ad libitum intake and ...200919502513
two major clades of bradyrhizobia dominate symbiotic interactions with pigeonpea in fields of côte d'ivoire.in smallholder farms of côte d'ivoire, particularly in the northeast of the country, cajanus cajan (pigeonpea) has become an important crop because of its multiple beneficial facets. pigeonpea seeds provide food to make ends meet, are sold on local markets, and aerial parts serve as forage for animals. since it fixes atmospheric nitrogen in symbiosis with soil bacteria collectively known as rhizobia, c. cajan also improves soil fertility and reduces fallow time. yet, seed yields remain low mostl ...201627891120
quality evaluation of stiff porridges prepared from irish potato (solanum tuberosum) and pigeon pea (cajanus cajan) starch blends.quality attributes of stiff porridges prepared from irish potato and pigeon pea starch blends were studied. starches were extracted from irish potato and pigeon pea using a wet extraction method. various ratios of the starches were mixed and analyzed for chemical, functional and pasting properties. the starch blends were then prepared into stiff porridges for sensory evaluation using a 20-man sensory panel. substitution of irish potato starch with pigeon pea starch led to increases in protein (0 ...201123729855
heterologous expression of legumin gene in e. coli isolated from cdna clones of immature seeds of pigeonpea (cajanus cajan l.).proteins are one of the targets for improving the nutritional quality, and attempts are being made through manipulation of its native gene(s). pigeonpea (cajanus cajan l.) is one of the nutritionally important legumes of tropical and subtropical regions of the world, and studies of the structure of seed storage proteins and their interactions have been limited by the difficulty of isolating single-protein subunits in large amounts from a complex mixture of the seed endosperm. one way to overcome ...200918597049
plantlet regeneration from decapitated embryonic axes of pigeonpea [cajanus cajan (l.) millsp.].an efficient plant regeneration protocol for embryonic axes of pigeonpea was developed so that this explant can further be used for agrobacterium mediated transformation. multiple shoots were produced from 15 hr soaked seed derived embryo axes without apical dome on modified. murashige and skoog (ms) medium supplemented with bap ( 1 mg l(-1)) and iaa (0.5 mg l(-1)) . regenerated shoots were rooted on ms medium supplemented with iba (0.3 mg l(-1)). the plantlets, thus obtained, were hardened and ...199919691174
reduction of a-galactoside content in red gram (cajanus cajan l.) upon germination followed by heat treatment.red gram (cajanus cajan l.) is an important crop for human and animal nutrition. however, raffinose family oligosaccharides present in red gram seed hinder its consumption as it is not digested by normal human carbohydrases and is further fermented by intestinal microflora, which induces flatulence. in order to make the grain legume more amenable for human consumption, we have tried to shed some light on the effect of germination followed by heat treatment methods such as autoclaving, cooking an ...201121452170
characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents.a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ...201525832181
protective effect of stilbenes containing extract-fraction from cajanus cajan l. on abeta(25-35)-induced cognitive deficits in mice.cajanus cajan (l.) is a traditional chinese herb medicine which contains a lot of potential active components. in the present study, we identified the effects of the stilbenes containing extract-fraction from c. cajan l. (secc) on abeta(25-35)-induced cognitive deficits, oxidative stress and cholinergic dysfunction in mice. mice were treated with secc (100 and 200mg/kg/d) for 1-week, and then received a single intracerebroventricular (i.c.v.) injection of abeta(25-35) (5mug/mice). behavioral cha ...200919833171
evaluation of the antifungal activity and modulation between cajanus cajan (l.) millsp. leaves and roots ethanolic extracts and conventional antifungals.the use and investigation of natural products with antimicrobial activity from vegeral source have been reported by several researchers. cajanus cajan (fabaceae) is a multiple use specie mainly as human food. in popular medicine, diverse parts of the plant are used as sedative and to treat cough, hepatitis, and diabetes.201222701281
effect of agricultural amendments on cajanus cajan (pigeon pea) and its rhizospheric microbial communities--a comparison between chemical fertilizers and bioinoculants.inoculation of leguminous seeds with bioinoculants has been practiced in agriculture for decades to ameliorate grain yield by enhanced growth parameters and soil fertility. however, effective enhancement of plant growth parameters results not only from the direct effects these bioinoculants impose on them but also from their non-target effects. the ability of bioinoculants to reduce the application of chemicals for obtaining optimum yield of legume appears to be of great ecological and economic ...201526231030
cajanol, a novel anticancer agent from pigeonpea [cajanus cajan (l.) millsp.] roots, induces apoptosis in human breast cancer cells through a ros-mediated mitochondrial pathway.cajanol (5-hydroxy-3-(4-hydroxy-2-methoxyphenyl)-7-methoxychroman-4-one) is an isoflavanone from pigeonpea [cajanus cajan (l.) millsp.] roots. as the most effective phytoalexin in pigeonpea, the cytotoxic activity of cajanol towards cancer cells has not been report as yet. in the present study, the anticancer activity of cajanol towards mcf-7 human breast cancer cells was investigated. in order to explore the underlying mechanism of cell growth inhibition of cajanol, cell cycle distribution, dna ...201020638373
effects of water extract of cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts.the effects of water extract of cajanus cajan (linn.) millsp. (leguminosae) leaves (wecml) on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (bmscs) and the adipocytic trans-differentiation of mouse primary osteoblasts (obs) were studied. the results indicated that wecml promoted the proliferation of bmscs and obs at most concentrations. wecml promoted the osteogenic differentiation and formation of mineralized matrix nodules of bmscs at concentrations o ...201020645761
development of new cytoplasmic-genetic male-sterile lines in pigeonpea from crosses between cajanus cajan (l.) millsp.and c. scarabaeoides (l.) thouars.exploitation of hybrid vigour is quite possible in cross-pollinated crops. however, pigeonpea is a grain legume crop with a moderate level of cross-pollination (20-70%), which is mainly aided by insect pollinators. as a first step, hybrids based on genetic male sterility (gms) were developed in pigeonpea, but the hybrid seed production technique is not farmer-friendly, because in the hybrid seed production plot 50% of the population, which are male-fertile in the female rows, have to be eliminat ...200818670057
conversion of cassava wastes for biofertilizer production using phosphate solubilizing fungi.two fungi characterized as aspergillus fumigatus and aspergillus niger, isolated from decaying cassava peels were used to convert cassava wastes by the semi-solid fermentation technique to phosphate biofertilizer. the isolates solubilized ca(3)(po(4))(2), alpo(4) and fepo(4) in liquid pikovskaya medium, a process that was accompanied by acid production. medium for the ssf fermentation was composed of 1% raw cassava starch and 3% poultry droppings as nutrients and 96% ground (0.5-1.5mm) dried cas ...201020138509
nutritive value, fermentation characteristics, and in situ disappearance kinetics of ensiled warm-season legumes and bahiagrass.this study determined the nutritive value, ensiling characteristics, and in situ disappearance kinetics of bahiagrass (paspalum notatum flügge 'tifton 9'), perennial peanut (arachis glabrata benth. 'florigraze'), annual peanut [arachis hypogaea (l.) 'fl mdr 98'], cowpea [vigna unguiculata (l.) walp. 'iron clay'], and pigeonpea [cajanus cajan (l.) millsp. 'ga-2']. all forages were harvested at maturity stages that optimized dry matter (dm) yield and nutritive value. after harvest, forages were wi ...201121426995
design and synthesis of cajanine analogues against hepatitis c virus through down-regulating host chondroitin sulfate n-acetylgalactosaminyltransferase 1.there still remains a need to develop new anti-hcv agents with distinct mechanism of action (moa) due to the occurrence of resistance to direct-acting antiviral agents (daas). cajanine, a stilbenic component isolated from cajanus cajan l., was identified as a potent hcv inhibitor by phenotypic screening in this work (ec50 = 3.17 ± 0.75 μm). the intensive structure optimization provided significant insights into the structure-activity relationships. furthermore, the moa study revealed that cajani ...201627783522
two rhizobacterial strains, individually and in interactions with rhizobium sp., enhance fusarial wilt control, growth, and yield in pigeon pea.a pseudomonas aeruginosa strain, rrlj 04, and a bacillus cereus strain, bs 03, were tested both individually and in combination with a rhizobium strain, rh 2, for their ability to enhance plant growth and nodulation in pigeon pea (cajanus cajan l.) under gnotobiotic, greenhouse and field conditions. both of the rhizobacterial strains exhibited a positive effect on growth in terms of shoot height, root length, fresh and dry weight, nodulation and yield over the non-treated control. co-inoculation ...201425224506
late embryogenesis abundant (lea) proteins in legumes.plants are exposed to different external conditions that affect growth, development, and productivity. water deficit is one of these adverse conditions caused by drought, salinity, and extreme temperatures. plants have developed different responses to prevent, ameliorate or repair the damage inflicted by these stressful environments. one of these responses is the activation of a set of genes encoding a group of hydrophilic proteins that typically accumulate to high levels during seed dehydration ...201323805145
expression of pigeonpea hybrid-proline-rich protein encoding gene (cchyprp) in yeast and arabidopsis affords multiple abiotic stress tolerance.a hybrid-proline-rich protein encoding gene (cchyprp) has been isolated and characterized, for the first time, from the subtracted cdna library of pigeonpea (cajanus cajan l.) plants subjected to drought stress. functionality of cchyprp has been validated for abiotic stress tolerance using the heterologous yeast and arabidopsis systems. the cchyprp contained a repetitive proline-rich (pr) n-terminal domain and a conserved eight cysteine motif (8cm) at the c-terminus. southern analysis disclosed ...201020055960
effects of soil solarization on nematodes parasitic to chickpea and pigeonpea.solarization by covering the soil with transparent polyethylene sheets during the summer months (april, may, june) in 1984 and 1985 significantly (p = 0.01) reduced the population densities of nematodes (heterodera cajani, rotylenchulus reniformis, helicotylenchus retusus, pratylenchus sp., and tylenchorhynchus sp.) parasitic to chickpea and pigeonpea. population density reductions of 93% of heterodera cajani eggs and juveniles, 99% ofhelicotylenchus retusus, 98% of pratylenchus sp., and 100% of ...199019287776
Characterization of five fungal endophytes producing Cajaninstilbene acid isolated from pigeon pea [Cajanus cajan (L.) Millsp].Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological ...201122102911
ensifer glycinis sp. nov., a rhizobial species associated with species of the genus glycine.rhizobial strains from root nodules of astragalus mongholicus and soybean (glycine max) were characterized phylogenetically as members of the genus ensifer (formerly named sinorhizobium), based on 16s rrna gene sequence comparisons. results based upon concatenated sequence analysis of three housekeeping genes (reca, atpd and glnii, ≤ 93.8 % similarities to known species) and average nucleotide identity (ani) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to ensifer fr ...201627125987
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