| fructose 1,6-bisphosphate aldolase activity of rhizobium species. | fdp aldolase was found to be present in the cell-free extracts of rhizobium leguminosarum, rhizobium phaseoli, rhizobium trifolii, rhizobium meliloti, rhizobium lupini, rhizobium japonicum and rhizobium species from arachis hypogaea and sesbania cannabina. the enzyme in 3 representative species has optimal activity at ph 8.4 in 0.2m veronal buffer. the enzyme activity was completely lost by treatment at 60 degrees c for 15 min. the km values were in the range from 2.38 to 4.55 x 10(-6)m fdp. met ... | 1975 | 283 |
| in vivo and in vitro activation of t-antigen receptors on leukocytes and platelets. | serological studies on a patient whose red cells are polyagglutinable due to t activation have demonstrated concomitant t activity of the separated leukocytes and platelets. normal leukocytes and platelets are not t active, but activation can be induced in vitro by treatment with neuraminidase or with pneumococcus type iii filtrate. such t-active cells absorb anti-t from arachis hypogea lectin. tests on idfferent types of separated leukocytes show that both neutrophils and lymphocytes have laten ... | 1976 | 12617 |
| a case of atypical polyagglutinability due to tk-transformation. | a case of acquired transient polyagglutinability in a group o, rh(d) positive female patient, wa, is described. an unusual finding in the preliminary serological investigation was the non-reaction of the cells with peanut (arachis hypogaea) extract. the patient's red cells were strongly agglutinated by normal human and rabbit sera from which anti-t had been removed. electrophoretic mobility studies and tests with polybrene indicated that their sialic acid content was normal. the use of a specifi ... | 1978 | 83151 |
| carbohydrate composition of peripheral, cultured and leukaemic human lymphocyte plasma membranes. | plasma membranes isolated from peripheral blood lymphocytes of normal donors, lymphocytes from patients with chronic lymphatic leukaemia (cll), a t cell and b cell line (molt-3 and rpmi-1788) were analysed and compared for total carbohydrate contents. t cells and peripheral blood lymphocytes contained the highest relative amounts of sialic acid and fucose, whereas chronic lymphatic leukaemic cells possessed the highest amounts of n-acetylgalactosamine and also more total cell surface carbohydrat ... | 1978 | 206031 |
| concomitant t- and tk-activation associated with acquired-b antigens. | three patients with acquired-b antigens are reported whose red blood cells also manifested t- and tk-activation. the results of tests using purified arachis hypogaea and bandeiraea simplicifolia (bs ii) lectins, and red blood cells either t, tk or both t and tk-activated by bacterial enzymes in vitro, suggest that the acquired-b phenomenon, t-activation and tk-activation result from the action of different bacterial enzymes. | 1979 | 222015 |
| new lectin receptors in carcinoembryonic antigen (cea). | the glycoprotein cea (carcinoembryonic antigen) carries carbohydrate groups, which react with the plant lectins from agaricus bisporus, arachis hypogaea (peanut), with tridacnin from invertebrate clams and with the anti-a lectins from snails. accordingly, it has cryptantigenic structures, which correspond to the t or t-like antigen, the tridacnin receptor and to the so called a-like antigen. | 1978 | 414933 |
| syntheses of o-beta-d-galactopyranosyl-(1 leads to 3)-0-(2-acetamido-2-deoxy-alpha(and -beta)-d-galactopyranosyl)-n-tosyl-l-serine and their interaction with d-galactose-binding lectins. | in order to use, as hapten inhibitors against various galactose-binding lectins, the derivatives of o-beta-d-galactopyranosyl-(1 leads to 3)-o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-l-serine, which is the common core structure of sugar chains of most mucins, the synthesis of these compounds was investigated. koenigs-knorr condensation of the 4,6-o-benzylidene derivative of o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-n-tosyl-l-serine methyl ester with 2,3,4,6-tetra-o-acetyl-alpha-d-g ... | 1979 | 427833 |
| response of irrigated groundnuts (arachis hypogaea l.) to urea fertilization in the central rainlands of the sudan. | the response of 3 varieties of groundnuts, i.e.: ashford (a), barberton (b), and corinte (c), to different doses of urea nitrogen under irrigation was studied for three years. as a result of extensive tissue analysis, the critical needs of barberton and corinte for nitrogen were found to be 1500 ppm no3-n and 3.7% total-n. variety ashford, which outyielded both, did not respond to urea nitrogen. a linear correlation coefficient of r = 0.85 for pod and straw yields gave a pod to straw ratio of 0. ... | 1979 | 473957 |
| investigations into rhizosphere microflora of some plants in libya. | the present investigation deals with the effect of raising hordeum vulgare and arachis hypogaea at two different stages on the microbial rhizosphere population. the studies were extended to reveal the microbiological occurrence in different horizons of a soil profile corresponding in length to root regions. bacteria were most abundant, followed by actinomycetes while fungi were less abundant and more restricted in their distribution. the three groups of microorganisms varied markedly in the rhiz ... | 1979 | 494853 |
| [receptors of human red blood cells for anti-tah and anti-ahp in the course of intravascular aging]. | human o-erythrocytes aged in situ do not expose free receptors for anti-t agglutinin of arachis hypogea. new receptors for concentrated anti-a agglutinin of helix pomatia are manifested but are lost again in the course of further ageing of the cells in situ. a remasking of exposed receptors for anti-tah and anti-ahp byautologous, strongly binding globulins is supposed. these globulins could constitute the decisive signal for the autologous phagocytosis of senescent red blood cells. | 1979 | 525143 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| lipid metabolism in chlorotic spot virus infected groundnut leaves (arachis hypogaea l var. tmv 2). | | 1977 | 612535 |
| rhizobium strain identification in arachis hypogaea nodules by enzyme-linked immunosorbent assay (elisa). | the technique of enzyme-linked immunosorbent assay (elisa) was used for serological identification of peanut rhizobium strains both in cell suspension of pure culture and in single root nodules of groundnut (arachis hypogaea) plants. antisera of three peanut rhizobium strains were tested against eight different rhizobium isolates. three serogroups identified by agglutination and immunodiffusion tests were confirmed by elisa. in this experiment elisa was more sensitive by four to six orders of ma ... | 1978 | 747814 |
| immuno-chemical studies on the alkali-labile carbohydrate chains of human serum glycoproteins. | human serum glycoproteins can be classified into those containing n-acetyl-d-galactosamine and into those lacking this hexosamine. the n-acetyl-d-galactosamine-containing serum glycoproteins have alkali-labile chains containing this hexosamine linked o-glycosidically to hydroxy amino acids. these alkali-labile chains can be demonstrated in neuraminic acid free serum glycoproteins by gas liquid chromatography and by using precipitating lectins from invertebrates and plants. they are represented b ... | 1979 | 762504 |
| the purification, composition, and specificity of the anti-t lectin from peanut (arachis hypogaea). | peanut agglutinin was purified by affinity chromatography on sepharose-epsilon-aminocaproyl-beta-d-galactopyranosylamine. the purified lectin obtained in a yield of 150 mg/100 g of defatted peanut was homogeneous on polyacrylamide gel electrophoresis, ultracentrifugation, and gel filtration. this intrinsic sedimentation coefficient (so20,w) and the intrinsic diffusion coefficient (do20,w) were estimated at ph 7.4 as 5.7 +/- 0.1 s and 5.0 x 10(-7) cm2s(-1), respectively. the molecular weight of t ... | 1975 | 811657 |
| a carbohydrate-deficient membrane glycoprotein in human erythrocytes of phenotype s-s-. | 1. we investigated the membranes of human erythrocytes which completely lack the blood-group antigens s and s (denoted as s-s-) as part of a study of the structure and function of the surface glycoproteins of the human erythrocyte. 2. the s-s-erythrocyte-membrane glycoprotein pas-3 band was much less intensely stained in comparison with that of the glycoprotein from normal erythrocyte membranes. the s-s-membrane glycoprotein pas-4 band also showed decreased staining. 3. examination with the lect ... | 1977 | 889570 |
| [the effect of the raw protein supply on the lysine requirements of young pigs of 12-40 kg. 1. report. feeding studies with wheat-peanut extraction residue rations]. | 10 feedings trials were carried out with a total of more than 500 pigs weighing 12 to 40 kgs. to investigate the lysine needs of growing pigs, the animals were fed rations of wheat + extracted ground nut meal. different food mixtures were tested containing 5 levels of crude protein (19%, 17%, 15%, 13% and 11% of the dry feed. within each crude protein level 6 graded lysine supplements were added to the ration. the trial showed that the lysine requirements of the weaned pigs were in a decisive me ... | 1976 | 1008705 |
| immunochemical studies on the specificity of the peanut (arachis hypogaea) agglutinin. | the specificity of purified, peanut agglutinin has been studied immunochemically by quantitative precipitin and inhibition assays. the lectin showed substantial differences in precipitating with blood-group substances of the same specificity. of the b substances tested, horse 4 25% completely precipitated the lectin, beach phenol insoluble failed to interact, and pm phenol insoluble gave an intermediate reaction. the lectin did not precipitate with a1 substances, with hog gastric mucin a + h sub ... | 1976 | 1033785 |
| [studies on the effect of raw protein supply on the lysine requirements in young pigs of living weight of 12-40 kg. 2. report. feeding studies with wheat-soy bean extraction residues]. | a feeding trial was conducted on a total of 96 pigs to investigate the effect of lysine supplements added to rations of wheat+extracted soya bean meal and rations of wheat+extracted gound nut meal. significant differences in the daily weight gains were noted (up to the 4th week of experiment) between the groups fed the soya bean meal ration and those fed the ground nut meal ration and between the soya bean meal groups receiving rations supplemented with 0,71% lysine or 0.79% lysine. at the termi ... | 1976 | 1034472 |
| immunochemical and chemical investigations of the structure of glycoprotein fragments obtained from epiglycanin, a glycoprotein at the surface of the ta3-ha cancer cell. | the structures of the carbohydrate chains present in fragments of a large-molecular-weight glycoprotein, epiglycanin, cleaved from the surface of viable ta3-ha murine mammary carcinoma ascites cells and purified by gel filtration, were studied by immunochemical and chemical methods. inhibitory activities for neuraminidase-treated and untreated glycoprotein material in the hemagglutination of nn-specific human erythrocytes by eight purified lectins were determined. excellent inhibition was obtain ... | 1975 | 1125949 |
| further characterization of some heterophile agglutinins reacting with alkali-labile carbohydrate chains of human erythrocyte glycoproteins. | the nature of the receptor sites for several agglutinins is characterized by hemagglutination inhibition assays. the inhibitory activity of human erythrocytes glycoproteins, from which sialic acid, sialic acid and galactose or alkali-labile oligosaccharides have been removed, is compared to the inhibitory effect of compounds with known structure. it is shown that the lectin from arachis hypogea and anti-t bind to alkali-labile galactosyl-residues. agglutinins from bauhinia purpurea and variegata ... | 1975 | 1172890 |
| [effect of alcohol on the metabolic conversion of 14c erucic acid in the liver of rats receiving rapeseed or peanut oil]. | the effects of addition of ethanol to diets containing rapeseed or ground nut oil on the metabolic conversions of 14 14c erucic and 9-10 3h oleic acid were studied in the rat liver. 1. whatever the diet more 14c than 3h radioactivity was recovered in liver lipids 2 and 19 hours after injection of labelled fatty acids. ethanol has little effect on this incorporation. 2. only small amounts of 3h oleic acid were converted. 3. in all cases, the metabolic conversion of erucic acid was identical: the ... | 1975 | 1221113 |
| effect of seed fumigation on the symbiosis of rhizobium sp. with arachis hypogaea linn. | | 1975 | 1242253 |
| protein and amino acid changes in peanut (arachis hypogaea l.) seeds infected with aspergillus oryzae. | | 1976 | 1245677 |
| [the effect of chlordecone (kepone) on the laboratory colonies of the pharaoh's ant monomorium pharanois]. | the control of the pharaoh's ant monomorium pharaonis is very difficult because of the social way of life in this insect pest. in regard to the reported good suppressing results of chlordecone we analyzed the mode of action in this compound at laboratory colonies of the pharaoh's ant. commercial gel and granular formulations as well as selfmade baits have been tested. the best results showed the granular bait on the basis of ground nut butter, while the effects of all of the others was much weak ... | 1976 | 1267219 |
| changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding. | lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) c57 bl/6j mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. the following lectins were used: canavalia ensiformis con a, triticum vulgaris (wga), glycine max (sba), griffonia simplicifolia (gs ii), arachis hypogaea (pna), pisum sativum (psa) and lens culinaris (lca). after incubation of frozen sections with con a, wga, gs ii, psa and lca a sarcoplasmic staining was obse ... | 1992 | 1290587 |
| modification of lectin binding in rat gut mucosa during experimental cholestasis. | glycoconjugate distribution on rat gut mucosa has been studied by using peroxidase-labelled lectins (lotus tetragonolobus, dolichos biflorus, arachis hypogaea and glycine max) after surgical interruption of the common bile duct. specimens from cholestatic rats were compared with sham-operated (simple laparotomy) and normal controls to determine which of the observed modifications could be due either to the operation itself or to the cholestasis. most of the modifications occurred in the duodenum ... | 1992 | 1295862 |
| in vitro formation and expansion of cysts derived from human renal cortex epithelial cells. | acquired renal cysts derive from terminally differentiated tubular epithelium in adults as a consequence of increased epithelial cell proliferation, fluid accumulation and extracellular matrix remodelling. to understand better how human epithelial cysts may be initiated and progressively expand, cells from primary cultures of normal human adult renal cortex were dispersed in polymerized type i collagen. the transparent matrix permitted repeated observation by light microscopy of cyst formation f ... | 1992 | 1319521 |
| presence of a vicia unijuga lectin-binding (vgu) glycoprotein with thomsen-friedenreich (t) activity and vgu glycoproteins in human primary hepatocellular carcinoma. | 1. twenty perchloric acid-soluble glycoprotein fractions (pasfs) were separated from carcinomatous and non-carcinomatous regions of the livers of 5 patients with primary hepatocellular carcinoma (hcc) and 5 patients with a complication of hcc and hepatocirrhosis (hc). 2. in autoradiography using 125i-labelled vicia unijuga lectin (vua) and 125i-labelled arachis hypogaea anti-t lectin as probes, 10 pasfs from carcinomatous regions of the livers of 10 patients gave 1 vgu glycoprotein with t activi ... | 1992 | 1330763 |
| influence of different substrates in detoxification activity of adult rat hepatocytes in long-term culture: implications for transplantation. | substrates used to immobilize hepatocytes for transplantation govern attachment and long-term metabolic activity of these cells. the choice of these substrates is based on the familiarity with proteinaceous materials that are constituents of the extracellular matrix. the use of substrates that recognize carbohydrates on the cell surface may provide an alternative method to attach adult mammalian hepatocytes. in this study, immobilized lectins on tissue culture plasticware were used to support he ... | 1992 | 1344292 |
| lectin staining studies on follicular atresia in house rat (rattus rattus). | topographic distribution of terminal and intercalary carbohydrate moieties were studied using horseradish peroxidase conjugated glycine max (gma), arachis hypogea (pna), lotus tetragonolobus (lta), bandirarea simplicifolia (bsa), and dolichos biflorus (dba) agglutinins. n-gal nac, alpha-d-gal, alpha-l-fuc, d-gal (beta-1-3)-d-gal nac, and alpha-beta-d gal nac linked glyco-conjugates were exposed during follicular atresia in oocyte, zona pellucida and granulosa cells. theca interna and interstitia ... | 1992 | 1365769 |
| a single change of histidine to glutamine alters the substrate preference of a stilbene synthase. | stilbene and chalcone synthases are related polyketide synthases which use the same substrates but form different products. the environment of the condensing active site cysteine is highly conserved, except for the positions -2 and -3. all chalcone synthases contain gln-gln and prefer 4-coumaroyl-coa as starter coa ester, while the two known stilbene synthases contain gln-his or his-gln (preference phenylpropionyl-coa and 4-coumaroyl-coa, respectively). we investigated whether the presence and/o ... | 1992 | 1400374 |
| localization and identification of t-(gal beta 1-3galnac alpha 1-o-r) and t-like antigens in experimental rat bladder cancer. | a mouse monoclonal antibody and a rabbit polyclonal antibody against the t-antigen (gal beta 1-3galnac alpha 1-o-r) were used to study the distribution of t-antigens in an experimental rat bladder cancer model. neoplasia was induced in 28 rats by intravesical installation of n-nitroso-n-methylurea (nmu) dissolved in acetate buffer. fifteen rats were installed with acetate buffer, and served as controls. urothelial samples were taken from all animals, the atypia was graded and detailed data on th ... | 1992 | 1404660 |
| nerve-induced secretion of glycoconjugates from cat submandibular glands: a correlative study with lectin probes on tissues and saliva. | horseradish peroxidase-conjugated lectins were used on tissue sections to localize the main secretory glycoproteins in cat submandibular glands and on western blots to evaluate their movement into saliva with selective nerve stimulation. central acinar cells bound lectins from arachis hypogaea (pna) specific for the terminal disaccharide gal beta 1, 3galnac, griffonia simplifolia (gsa i-b4) specific for terminal alpha gal, and lotus tetragonolobus (lta) specific for fucose. lectins from limax fl ... | 1992 | 1431061 |
| histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows. | the epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three holstein-friesian cows affected with chronic polypoid cystitis. except for ricinus communis (rca-i), almost all goblet cells in the whole crypt were positive to periodic acid-schiff (pas), alcian blue (ab) ph 2.5, ab ph 1.0, periodate borohydride-potassium hydroxide-pas (pb-koh-pas), ulex europaeus (uea-i), triticum vulgare (wga) and arachis hypogaea (pna) after neuraminidase digestion. t ... | 1992 | 1452812 |
| temporal lectin histochemical characterization of porcine small intestine. | a variety of biotinylated lectins was applied to formalin-fixed intestinal sections from isolator-reared pigs ranging in age from newborn through 12 weeks. lectin binding to brush borders of villus enterocytes, crypt enterocytes, and dome epithelium, and lectin reactivity within goblet cells and brunner's glands was semiquantified by microscopy and was used to estimate temporal changes in complex carbohydrates of enteric epithelium. although variability in binding scores often was observed among ... | 1992 | 1456535 |
| serological and biochemical studies on en(a-) human erythrocytes in a japanese family. | the propositus erythrocytes with phenotype en(a-), which was found in the first example of a japanese family, reacted with anti-n serum weaker than the ordinary phenotype n erythrocytes. when the erythrocyte membranes of the propositus were subjected to sds-page, no glycophorin a was observed on the gel by pas staining, whereas glycophorin b band was observed. the s and the s antigens of the propositus erythrocytes were appeared to be normal. these results suggested that n activity of the propos ... | 1992 | 1460794 |
| human sperm surface mapping with lectins. | ten fluorescein isothiocyanate (fitc)-linked lectins [bauhimia purpurea, concanavalin a, dolichos biflorus (dba), griffonia simplicifolia i, griffonia simplicifolia ii, maclura pomifera, arachis hypogea (pna), glycine max, ulex europaeus (uea) and triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. furthermore, ultrastructural loc ... | 1992 | 1466231 |
| immunotoxic responses of cypermethrin, a synthetic pyrethroid insecticide in rats. | the study was undertaken to evaluate immunotoxic effects of cypermethrin administered orally (in ground nut oil) to male albino rats at dose levels (mg/kg) of 0 (control), 5, 10, 20 and 40 once daily for 90 days. cypermethrin administration produced a significant leucopenia at 40 mg/kg on day 90. a dose dependent decrease (p greater than 0.05) in delayed type hypersensitivity reaction was noticed on day 61 post treatment. humoral response as evidenced by serum haemagglutinin and haemolysin titre ... | 1992 | 1506076 |
| human urinary bladder carcinoma glycoconjugates expressing t-(gal beta(1-3)galnac alpha 1-o-r) and t-like antigens: a comparative study using peanut agglutinin and poly- and monoclonal antibodies. | t- and t-like antigens on glycoproteins and glycolipids were examined in extracts of human urinary bladder tumors and normal tissue by western blot analysis and reagent binding to thin layer chromatograms. three different anti-t-reagents were used: peanut (arachis hypogaea) lectin (pna) and mono- and polyclonal antibodies specific for t-antigen (gal beta(1-3)galnac alpha 1-o-r). immunodetection with the t-specific reagents in nitrocellulose replicas of bladder tumor glycoproteins, separated by s ... | 1992 | 1516058 |
| isolation and characterization of a lectin from peanut roots. | a glucose-specific lectin has been purified to apparent homogeneity from 7-day-old peanut (arachis hypogaea) roots by affinity chromatography on a sephadex g-50. the lectin has a 66 kda native molecular mass and a 33 kda subunit molecular mass as revealed by native and denaturing sodium dodecyl sulphate-polyacrylamide gel electrophoresis, respectively. the purified lectin, gives a single precipitin line with the antiserum produced against 7-day-old root extract and shows 5 bands in the ph range ... | 1992 | 1525173 |
| molecular analysis of chalcone and dihydropinosylvin synthase from scots pine (pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants. | chalcone synthase (chs) and stilbene synthase (sts) are closely related polyketide synthases which are key enzymes in the biosynthesis of flavonoids and stilbenes. scots pine (pinus sylvestris) is an interesting plant for a direct comparison of the enzymes. it not only contains the usual flavonoids, but also an unusual chalcone derivative (pinocembrin), and it synthesizes stilbenes of the pinosylvin type. we analysed a chs and a sts by molecular cloning and functional expression in escherichia c ... | 1992 | 1536925 |
| effect of lectins on hepatic clearance and killing of candida albicans by the isolated perfused mouse liver. | the isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of candida albicans. after mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) c. albicans cfu were infused into the livers. at the time of recovery, 63% +/- 2% (mean +/- standard error of the mean) of the infused c. albicans cfu were recovered from the liver and 14% +/- 1% were recovered from the effluent for a total recovery of 77% +/- 2%. this indicated tha ... | 1992 | 1541519 |
| flow cytometric analyses of lectin binding to pneumocystis carinii surface carbohydrates. | pneumocystis carinii obtained from infected rat lung homogenates was incubated with fluorescein isothiocyanate-conjugated lectins, counterstained with the nuclear stain, propidium iodide (pi), and analyzed by dual parameter histograms for lectin-associated green and pi-associated red fluorescence using a fluorescence-activated cell sorter. the presence of glucose/mannose moieties was evidenced by the binding of all organisms to concanavalin a and wisteria floribunda. from the lectin group specif ... | 1992 | 1556643 |
| carbohydrates in the cell surface of hair cells from the guinea pig cochlea. | the presence of cell surface carbohydrates was investigated in intact, non-fixed outer hair cells (ohcs) of guinea pigs using fluorescein isothiocyanate (fitc) and rhodamine (tritc) lectins. by means of wheat germ agglutinin (wga) n-acetyl-d-glucosamine was shown in the entire ohc membrane, including the stereocilia. binding of wga in ohcs to neuraminic acid was excluded by preincubation with neuraminidase. moreover, fitc-limulus polyphemus, a specific lectin for neuraminic acid, showed no fluor ... | 1992 | 1581047 |
| isolation and characterization of a vicia graminea and vicia unijuga lectins-binding (vgu) glycoprotein with thomsen-friedenreich (t) activity from human liver metastases of pancreas carcinoma. | 1. perchloric acid-soluble fraction from liver metastases of pancreas carcinoma of a patient with blood group b, was subjected to a systematic affinity chromatography using vicia unijuga lectin (vua) and arachis hypogaea anti-t lectin (pna) as immobilized ligands and separated into three fractions, b-active glycoprotein fraction, serologically inactive glycoprotein fraction and glycoprotein (vp) fraction exhibiting reactivities for vicia graminea lectin (vga), vua and pna. 2. vga- and vua-bindin ... | 1992 | 1592152 |
| intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh. | to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ... | 1992 | 1592875 |
| characterization of bone forming cells in posttraumatic myositis ossificans by lectins. | lectins were used to characterize bone forming cells in posttraumatic myositis ossificans. the lectins applied were as follows: arachis hypogaea (pna): specific for beta-d-galactose (1,3)n-acetyl-d-galactosamine (gal-1,3 galnac), canavalia ensiformis (con a): specific for alpha-d-glucose (d-glc) and alpha-d-mannose (d-man) and wheat germ (wga): specific for n-acetyl(1,4)d-glucosamine (glc-nac) and neuraminic acid. the development of myositis ossificans was characterized by the appearance of a wg ... | 1992 | 1594488 |
| tissue binding pattern of plant lectins in benign and malignant lesions of thyroid. | n-acetyl d-galactosamine specific lectins were isolated from the seeds of jack fruit (artocarpus integrifolia) and winged bean (psophocarpus tetragonolobus) and d-galactose specific lectin was isolated from peanut (arachis hypogaea). these lectins were conjugated to horse radish peroxidase (hrp) and were used to study the lectin binding properties of benign and malignant lesions of the thyroid. for comparison of the results 10 normal fresh autopsy specimens were included in the study. the peanut ... | 1992 | 1625034 |
| histochemical analysis of rat testicular glycoconjugates. 2. beta-galactosyl residues in o- and n-linked glycans in seminiferous tubules. | rat testes have been examined with a panel of lectins that bind specifically to oligosaccharide sequences having terminal or subterminal beta-galactosyl residues in o-linked glycans, or in the outer chains of complex n-linked glycans: arachis hypogaea (peanut, aha), erythrina cristagalli (coral tree, eca), ricinus communis (castor bean, rca120) and abrus precatorius (jequirity bean, apa) agglutinins. pretreatment of sections with neuraminidase, beta-galactosidase and removal of alkali-labile o-l ... | 1992 | 1634372 |
| changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters. | lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ... | 1990 | 1700650 |
| lectin histochemical characteristics of the canine female mammary gland. | twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ... | 1990 | 1700652 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| lectin histochemistry on squamous metaplasia in different epithelial tumors of dogs. | biotinylated lectins and avidin-biotin-peroxidase complex were used to study the correlation between cellular glycoconjugates' expression and squamous maturation in normal canine skin and in various epithelial neoplasms. normal skin tissue was obtained from five, male, random-source dogs, 5 to 7 years old. the tumors tested, selected from the files of our department, were fifteen squamous cell carcinomas from different tissue origin, five hepatoid perianal gland adenocarcinomas with squamous met ... | 1991 | 1708179 |
| subcompartment sugar residues of gastric surface mucous cells studied with labeled lectins. | we examined the intracellular localization of sugar residues of the rat gastric surface mucous cells in relation to the functional polarity of the cell organellae using preembedding method with several lectins. in the surface mucous cells, the nuclear envelope and rough endoplasmic reticulum (rer) and cis cisternae of the golgi stacks were intensely stained with maclura pomifera (mpa), which is specific to alpha-gal and galnac residues. in the golgi apparatus, one or two cis side cisternae were ... | 1991 | 1708750 |
| primary cultures of normal rat kidney proximal tubule epithelial cells for studies of renal cell injury. | normal rat kidney proximal tubule epithelial cell cultures were obtained by collagenase digestion of cortex and studied for 10 days. to assess the purity of the seeding suspension, we histochemically demonstrated gamma-glutamyltranspeptidase in greater than 95% of the starting material. to identify cell types in cultures, we investigated several markers. cells stained positively for lectin arachis hypogaea (rat proximal tubule) and negatively for lotus tetragonolobus (rat distal tubule). interme ... | 1991 | 1717431 |
| lectin histochemistry of human fetal notochord, ecchordosis physaliphora, and chordomas. | lectin histochemical studies of human fetal notochord, ecchordosis physaliphora, and eight chordomas were performed. ecchordosis physaliphora and eight chordomas were stained with ricinus communis type i, canavalia ensiformis, triticum vulgaris, and limax flavus. ricinus communis type i, t vulgaris, and l flavus reacted with both tumor cells and the extracellular mucinous matrix, while c ensiformis mainly reacted with the cytoplasm of tumor cells. thus, tumor cells were most recognizable with th ... | 1992 | 1734835 |
| presence of terminal n-acetylgalactosamine residues in subregions of the endoplasmic reticulum is influenced by cell differentiation in culture. | using helix pomatia lectin as a specific probe for terminal, nonreducing n-acetylgalactosamine residues, glycoprotein precursors bearing newly initiated o-linked oligosaccharides have been localized in the lumen of the endoplasmic reticulum and cis-golgi cisternae. this pattern contrasts with the detection of the terminal disaccharide galactose beta-1,3-n-acetylgalactosamine by arachis hypogaea lectin in middle and trans-golgi compartments, which are considered elongation sites for o-glycosylati ... | 1991 | 1748669 |
| production & formulation of bacillus thuringiensis var. israelensis & b. sphaericus 1593. | three fermentation media each for bulk growth of b. thuringiensis var. israelensis and b. sphaericus 1593 were formulated using defatted groundnut cake (arachis hypogea) as the first nitrogen source and gram flour (cicer arientinum), soy bean (glycine max) and defatted milk powder as the second nitrogen source. medium containing gram flour showed highest toxicity (14.45 micrograms/l) in case of b. thuringiensis var. israelensis whereas medium containing milk powder was found to be highly toxic w ... | 1991 | 1778620 |
| separation of beta-cells from dispersed porcine pancreas by selective lectin binding. | variation in cell-surface sugar residues which exist between different pancreatic cells has been exploited in an attempt to isolate beta-cells from dispersed porcine pancreas utilizing selective lectin binding. the binding characteristics of a range of lectins were compared to determine their ability to differentiate between endocrine and non-endocrine cells in the porcine pancreas. histological analysis showed that peroxidase labelled arachis hypogaea bound selectively to islet cells in carnoy- ... | 1991 | 1816975 |
| lectin-binding patterns in the spermatogenic cells of the shiba goat testis. | lectin-binding patterns in the testis of the sexually mature goat were investigated by light and transmission electron microscopy. dolichos biflorus agglutinin (dba) and griffonia simplicifolia agglutinin-i (gs-i) were negative in the seminiferous epithelium, but soybean (glycine max) agglutinin (sba), griffonia simplicifolia agglutinin-ii (gs-ii) and peanut (arachis hypogaea) agglutinin (pna) were positive in the acrosomal vesicle of the golgi-phase spermatids and in the acrosome of the cap-, a ... | 1991 | 1836378 |
| induced plant responses to pathogen attack. analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (glycine max l. merr. cv. harosoy 63). | in soybean (glycine max l.), pathogen attack induces the formation of glyceollin-type phytoalexins. the biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. screening of a soybean cdna library from elicitor-induced rna in lambda gt11 yielded two classes of reductase-specific clones. the deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. four clones of class a were ... | 1991 | 1840523 |
| lectin histochemistry of trachea and lung of healthy turkeys (meleagris gallopavo) and turkeys with pneumonia. | thirteen lectins were used to characterize lectin-binding specificity of glycoconjugates on sections of formalin-fixed lung and trachea from seven normal turkeys, two turkeys with acute pneumonia, and two turkeys with chronic pneumonia. neuraminidase was used to digest sialic acid residues. one n-acetylgalactosamine-binding lectin and two n-acetylgalactosamine/galactose-binding lectins stained the apical membrane and cytoplasm of multifocal cells that lined air atria and hyperplastic granular ce ... | 1991 | 1858252 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| differential adhesion of pseudomonas aeruginosa to human respiratory epithelial cells in primary culture. | human nasal polyps in outgrowth culture were used to study the pseudomonas aeruginosa adhesion to respiratory cells. by scanning electron microscopy, p. aeruginosa were seen associated with ciliated cells, but by transmission electron microscopy, bacteria were never seen at the interciliary spaces or attached along cilia, but were identified trapped at the extremities of cilia, usually as bacterial aggregates. a fibronectin-containing fibrillar material was seen associated with aggregated bacter ... | 1991 | 1904070 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |
| elicitor-specific induction of one member of the chitinase gene family in arachis hypogaea. | chitinases are believed to play an important role in plant defence against bacterial and fungal attack. in peanut (arachis hypogaea) chitinase genes form a small multigene family. four chitinase cdnas (chit 1-4) were isolated from cultured peanut cells. expression of individual chit genes was assayed by the polymerase chain reaction (pcr) followed by analysis of restriction fragment length polymorphisms (rflp). uv irradiation, dilution of cell cultures and treatment with phytophthora megasperma ... | 1990 | 1980004 |
| comparison of serum anti-band 3 and anti-gal antibody binding to density-separated human red blood cells. | this study examines the quantitative relationship between two natural serum antibodies, anti-band 3 and anti-alpha-galactosyl (anti-gal), in their capacity to bind to human red blood cell (rbc) populations separated on density gradients. the question was approached in two ways. first, we determined the extent of rebinding of affinity-purified human serum antibodies to rbcs that had been stripped of in situ antibody. second, we eluted the in situ bound antibody at low ph from density-separated rb ... | 1991 | 1991171 |
| localization of lectin-binding sites and sugar-binding proteins in tachyzoites of toxoplasma gondii. | lectins and neoglycoproteins labeled with colloidal gold particles were used for the ultrastructural localization of carbohydrate residues and sugar-binding sites, respectively, in thin sections of tachyzoites of toxoplasma gondii embedded in the lowicryl k4m resin. incubation of the sections in the presence of gold-labeled canavalia ensiformis (con a), arachis hypogaea (pna), ricinus communis i (rca i), triticum vulgaris (wga), and limax flavus (lfa) agglutinins showed significant labeling of t ... | 1991 | 1992086 |
| health teaching in the context of culture: nursing in east africa. | a nurse from the us worked as a community health nurse with the mennonite central committee in east africa from 1981-1983. she addressed the importance of health care providers being able to incorporate the context of culture, religion, and socioeconomic status into identifying health care needs and intervention. this was complicated by differences in values and beliefs between the urban and rural populations. many people believed that spirits of the dead influence their behavior and sense of ... | 1991 | 2046219 |
| the behavior of human ameloblastoma tissue in collagen matrix in vitro: an immunohistochemical study. | we investigated the three-dimensional outgrowth of human ameloblastoma cells cultured in collagen matrix. growth patterns of four cases of ameloblastoma resembled each other and was characterized by appeared duct-like structures. case 1 ameloblastoma was subcultured 3 times. ameloblastoma tissues and cultured cells were stained with various lectins by avidin-biotin peroxidase complex (abc) staining methods. both the tissues and cultured cells had the same receptors to concanavalin ensiforme (con ... | 1991 | 2061858 |
| alterations in lectin binding in the proximal and distal colon of sprague-dawley rats with 1,2 dimethylhydrazine administration. | sequential changes in rhodamine or fluorescein isothiocyanate-conjugated lectin binding of proximal and distal colonic crypts were studied during and after the administration of the 1,2 dimethylhydrazine (dmh). five adult its unexposed to dmh or vehicle served as baseline controls. tissue from normal appearing colon and tumor tissue was incubated with ulex europaeus agglutinin 1 (uea), arachis hypogaea (pna), dolichos biflorus (dba) and griffonia simplicafolia 1 (gsa1). distinct regional differe ... | 1991 | 2070840 |
| expression of a stilbene synthase gene in nicotiana tabacum results in synthesis of the phytoalexin resveratrol. | a gene from groundnut (arachis hypogaea) coding for stilbene synthase was transferred together with a chimaeric kanamycin resistance gene. it was found to be rapidly expressed after induction with uv light and elicitor in tobacco cells (nicotiana tabacum). comparative studies of stilbene synthase mrna synthesis in groundnut and transgenic tobacco suspension cultures revealed the same kinetics of gene expression. stilbene synthase specific mrna was detectable 30 minutes after elicitor induction a ... | 1990 | 2103451 |
| effect of gamma-interferon on lectin-binding glycoproteins in cultured human keratinocytes. | we report the effect of exposure of human keratinocyte cultures to human recombinant gamma-interferon (g-ifn) on the expression of glycoproteins. concanavalia ensiformis agglutinin (con-a), and arachis hypogaea agglutinin (pna) were used to investigate expression of glycoproteins. np-40 extracts from cultures grown with or without 100 u/ml g-ifn were analyzed by incubation of sds-polyacrylamide gels with 125i-labeled lectins. comparison of con-a binding glycoprotein profiles showed both qualitat ... | 1990 | 2115319 |
| lectins and heparin-binding features of human spermatozoa as analyzed by flow cytometry. | sperm cell surface characteristics may serve as a useful diagnostic feature for the evaluation of seminal samples, but to date no useful analytical method(s) is available for their routine quantitative analysis. fluorescein isothiocyanate-conjugated lectins, proteins with unique and specific saccharide-binding characteristics, and heparin are potentially useful markers for such an analytical procedure. a protocol is outlined whereby frozen semen is thawed and treated with either fluorescein isot ... | 1990 | 2124086 |
| [a study of the t-antigen in ovarian tumor]. | by means of the avidin-biotin-peroxidase complex (abc) method with peanut lectin (pna, arachis hypogaea), the role of t-antigen in the human ovarian tumor was investigated and the following results were obtained. 1) among benign tumors, 76.9% cases were t-antigen negative and cryptic t-antigen positive. 2) among malignant ovarian tumors, 55.2% cases were t-antigen positive and 20.7% cases were negative for both t-antigen and cryptic t-antigen. 3) in malignant ovarian tumors, the lower the tissue ... | 1990 | 2153744 |
| autosomal recessive polycystic kidney disease: characterization of human peritoneal and cystic kidney cells in vitro. | renal cystic epithelia and peritoneal mesothelia from two humans with autosomal recessive polycystic kidney disease (arpkd) were grown in culture. cystic epithelial and mesothelial cells formed continuous monolayers in vitro. by electron microscopy, cystic renal cells exhibited a single apical cilium and numerous short, stubby microvilli, both in situ and in vitro. mesothelial cells exhibited intra- and extracellular membrane-limited, lipid-filled vesicles and surface microvilli. cystic kidney c ... | 1990 | 2154094 |
| characterization of an in vitro system of human renal papillary collecting duct cells. | we have developed an in vitro model of human papillary collecting duct cells isolated from cadaver kidneys using methods similar to those we previously reported for the isolation of human proximal tubule cells. to date we have isolated papillary collecting duct cells from 100 normal human kidneys. papillae were dissected and digested in cellgro containing 400 u/ml collagenase. cells were plated on fibronectin-coated culture flasks at a density of 10(4) live cells/ml in cellgro supplemented with ... | 1990 | 2161826 |
| [separation of thomsen-friedenreich (t) antigen and n antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties]. | one m perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group ab) with ovarian clear cell carcinoma in malignant was subjected to sephacryl s-200 gel filtration and fraction 1 which was eluted at void volume, reacted with both of the lectins of vicia unijuga and arachis hypogaea. this fraction was then separated into 6 fractions (deae-frs. 1-6) in a deae-sephacel column chromatography. deae-frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and ... | 1990 | 2165527 |
| biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins. | 1. perchloric acid-soluble glycoprotein fractions (pasfs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (ocas) in benign and borderline and ovarian clear cell carcinoma (occ) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. these pasfs reacted with anti-a, -b, -lea and/or -leb sera, but did not react with anti-h, -m and -n sera. all of the pasfs reacted ... | 1990 | 2165928 |
| application of flow cytometry to studies on the human acrosome. | this study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a fitc-labelled plant lectin (arachis hypogea agglutinin). localization of the label to the acrosome was encouraged by freezing the sperm for at least 24 hours at -70 degrees c prior to labelling. studies of sperm from 53 normospermic men revealed that acrosome labelling followed a single normal distribution without the presence of subpopulations. the average fluorescence and degree ... | 1990 | 2211342 |
| lectin typing of campylobacter isolates. | isolates of campylobacter jejuni, c coli, c fetus and c laridis were tested for agglutination reactions with a panel of five lectins: arachis hypogaea, bauhinia purpurea, solanum tuberosum, triticum vulgaris and wisteria floribunda. twenty three patterns of agglutination (lectin types) were recorded among 376 isolates. patterns were consistent and reproducible. only 4.5% of isolates were untypable because of autoagglutination. some lectin types were found exclusively or predominantly in a specie ... | 1990 | 2262570 |
| stimulation of vascular cell proliferation by beta-galactoside specific lectins. | an investigation was conducted to assess the effects of various beta-galactoside specific lectins on the growth of vascular cells in vitro. the plant lectins from peanut (arachis hypogaea), mushroom (agaricus bisporus), and coral tree (erythrina corallodendron) were used in these studies with the ultimate purpose of comparing those findings with data derived with the lectin isolated from rat lung. peanut lectin was added to confluent and subconfluent cultures of smooth muscle cells (smc), pulmon ... | 1990 | 2379767 |
| differences in expression of oligosaccharide determinants by phenotypically distinct sublines of the dunning 3327 rat prostate cancer. | oligosaccharides expressed by the 3327-h and 3327-mat lylu sublines of the dunning rat prostate cancer model have been compared in formalin-fixed and routinely paraffin-embedded tumour tissues. binding by lectins of defined specificity has been employed to identify expression of seven oligosaccharide structures by primary and metastatic prostatic carcinoma cells. neuraminidase digestion was employed to reveal determinants masked by sialic acid. the presence of core man alpha 1----3(man alpha 1-- ... | 1990 | 2388349 |
| effect of peanut tannin extracts on growth of aspergillus parasiticus and aflatoxin production. | twenty-three peanut (arachis hypogaea l.) genotypes were evaluated for kernel resistance to aspergillus parasiticus spear. colonization and aflatoxin contamination when incubated under high relative humidity. also, tannin-containing extracts from kernel coats (testae) and cotyledons of these genotypes were prepared and tested for their effect on a. parasiticus growth and aflatoxin production in vitro. the lowest degree of colonization, less than 30%, was noted in kernels from the genotypes, toal ... | 1990 | 2388679 |
| glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa. | an attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. this glycoconjugate is located on the innermost luminal surface, but its function is still unknown. the lectins evaluated included triticum vulgare, canavalia ensiformis, maclura pomifera, arachis hypogaea, ricinus communis i (rca-i), and limax flavus. the staining results obtained with the six different lectin probes in the lowicryl k4m-embedded g ... | 1990 | 2393562 |
| [monospecific antibodies against synthetic t-antigen. characteristics of their specificity and use in the identification of t-antigenic determinants on the cell surface]. | monospecific antibodies directed to a thomsen-friedenreich antigen (t-antigen) were obtained using artificial antigen. t-antigen immunodominant alpha-disaccharide galbeta (1----3) galnac alpha 1-(t alpha) and its beta-anomer gal beta (1----3) galnac beta 1-(t beta) were bound to bovine serum albumin (bsa) and cytochrome c (ccc) through a spacer (sp = -o(ch2)3nhco (ch2)4co-) by the azide method to give neoglycoproteins t alpha-sp-bsa, t alpha-sp-ccc and t beta-sp-bsa. anti-t alpha antiserum was o ... | 1985 | 2413865 |
| alterations in glomerular lectin binding sites of human kidney as detected by fluorescence microscopy. | different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. arachis hypogaea agglutinin (peanut lectin, pna), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. concomitantly with the appearance of pna-binding, binding sites for wheat germ agglutinin (wga) appeared to be lost at glomeruli. furthermore, changes in the expression of glomer ... | 1985 | 2415488 |
| analyses of avidin-biotin complexes with lectins of membrane glycoproteins in the urinary bladder of rats treated with n-butyl-n-(4-hydroxybutyl)nitrosamine. | the bladder epithelium was examined by staining with avidin-biotin complexes with lectins to determine the early membrane changes during bladder carcinogenesis in rats treated with n-butyl-n-(4-hydroxybutyl)-nitrosamine (bhbn). concanavalia ensiformis (con a), triticum vulgaris (wga), ricinus communis (rca) and arachis hypogaea (pna) were used as probes. the cellular distributions of lectin particles were distinguished into membranous and cytoplasmic patterns. normal bladder cells stained very s ... | 1987 | 2432280 |
| lectin binding to human endometrial hyperplasias and adenocarcinoma. | arias-stella change, cystic glandular hyperplasia, adenomatous hyperplasia, atypical hyperplasia, and adenocarcinoma of the human endometrium were stained with different fluorescein-conjugated lectins. the lectins used were concanavalin a, triticum vulgaris, arachis hypogaea, glycine maximum, dolichos biflorus, ulex europaeus, and ricinus communis. the results showed that the lectin staining of arias-stella change differed from the other conditions and resembled more closely the staining propert ... | 1987 | 2447025 |
| cytochemical approach to the development and behaviour of rat alveolar type ii cells in culture. | the development and characteristics of rat alveolar type ii cells were monitored by using various cytochemical techniques. polarized light microscopy was found useful for observing live type ii cells in culture. cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. similar results were obtained by using an immunocytochemical approach with antibodies raised against t ... | 1988 | 2450083 |
| lectin reactivity in human large bowel. | the affinity of six lectins (arachis hypogaea, concanavalin a, bandeiraea simplicifolia i. dolichus biflorus, triticum vulgaris and lotus tetragonolobus) was studied in 10 normal human large intestines. the results showed that each lectin had its own characteristic staining pattern. they differed in their affinity for different cellular components, local distribution within crypts and regional distribution between right (ascending colon) and left (rectum) segments of the large bowel. arachis hyp ... | 1987 | 2451203 |
| fragmentation of the beta-subunit of human chorionic gonadotropin produced by choriocarcinoma. | when human chorionic gonadotropin (hcg) was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions with dithiothreitol (dtt), a smaller weight material (ctp'), in addition to the beta-subunit, could be detected by western blot analysis using antiserum for hcg beta-carboxy-terminal peptide (ctp). the ctp' band was much more apparent with urinary hcg from a patient with choriocarcinoma than with that from normal pregnant women. second-dimensional electroph ... | 1988 | 2454807 |
| purification and characterization of a differentiation-specific sialoglycoprotein of lactating-guinea-pig mammary tissue. | a large acidic glycoprotein, pas-i, was purified from the fat-globule membrane of guinea-pig milk. threonine and serine accounted for over 30 mol% of the amino acids, and galactose, n-acetylgalactosamine, n-acetylglucosamine, mannose and sialic acid were the principal sugars detected. on a molar basis, sialic acid accounted for over 60% of the total sugar. removal of sialic acid by treatment with neuraminidase revealed the presence of binding sites for peanut (arachis hypogaea) agglutinin, a lec ... | 1988 | 2456756 |
| glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins. | the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ... | 1988 | 2466021 |
| cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland. | the glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. hexose residues of glycoconjugates were identified using ferritin conjugated with canavalia ensiformis agglutinin (con a), triticum vulgare or wheat germ agglutinin (wga), ricinus communis i agglutinin (rca-i), phaseolus vulgaris agglutinin (pha-e) and arachis hypogaea agglutinin (pna). whereas ... | 1989 | 2470700 |
| isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells. | cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ... | 1989 | 2486452 |
| adherence of pseudomonas aeruginosa to tracheal epithelium. | adherence of mucoid and nonmucoid strains of pseudomonas aeruginosa to tracheal epithelium was studied with a perfused-trachea model. the species specificity of adherence was studied by infecting tracheas from hamsters, guinea pigs, or mice. perfused tracheas from hamsters were infected with strains of p. aeruginosa in the presence of various sugars, lectins, cations, or charged polymers. adherence of mucoid strains of p. aeruginosa was greatest for guinea pigs; that for hamsters and mice was ap ... | 1989 | 2494109 |
| regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins. | regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ... | 1989 | 2505446 |
| [some chemical and serological properties of non-dialysable fractions of human seminal plasmas]. | some chemical and serological properties of water-soluble and undialysable fraction (wsf) and water-insoluble and undialysable fraction (wisf) of human seminal plasmas were studied. both of the fractions contained proteins as the main components and also carbohydrates as minor components, and each fraction gave 9 bands which were stained with the periodate-schiff and/or coomassie brilliant blue reagents on sodium dodecyl sulfate-polyacrylamide slub gel electrophoresis. wsf reacted with 17 of 19 ... | 1989 | 2515272 |